The Diagenode D-Plex Total RNA-seq Library Preparation kit is a tool designed for the study of the whole coding and non-coding transcriptome. The present kit incorporates the unique D-Plex technology to generate directional RNA libraries for Illumina sequencing directly from total RNAs, messenger RNAs (mRNAs) that have already been enriched by poly(A) capture, or RNAs that have already been depleted of ribosomal RNAs (rRNAs).