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Positive and negative spike-in controls are included for the monitoring of bisulfite conversion efficiency.
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Size selection has been optimized to keep small fragments of interest and to remove adaptor dimers, resulting in a better coverag.
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The pooling protocol includes a quantification of the samples and a pooling application, available on our website, to help you to choose the groups, depending on the DNA amount and adaptor barcode of each sample.
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The bisulfite conversion protocol has been improved to decrease DNA degradation while keeping a highly efficient conversion of unmethylated cytosine.
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The minimum number of amplification cycles needed for each pool is determined to avoid amplification biases. Our MethylTaq Plus enzyme was developed to amplify bisulfite converted DNA with high efficiency, and reduces the number of PCR cycles required.
