acrIIA4 monoclonal antibody datasheet DATASHEET Monoclonal antibody raised in mouse against acrIIA4 (anti CRISPR protein IIA4) using a recombinan... | Download |
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Lot | 002 |
---|---|
Concentration | 1.6 µg/µl |
Species reactivity | Listeria monocytogenes |
Type | Monoclonal |
Purity | Protein A purified monoclonal antibody |
Host | Mouse |
Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles |
Storage Buffer | PBS containing 0.05 % Na-azide. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Applications |
Suggested dilution/amount |
References |
Western blotting |
1:1,000 |
Fig 1 |
Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against acrIIA4
Western blot was performed on protein extracts from U2OS cells spiked with the full length recombinant acrIIA4 protein (lane 1), using the Diagenode antibody against acrIIA4 (Cat. No. C15200248), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. The marker is shown on the left, the position of the acrIIA4 protein is indicated on the right.
acrIIA4 monoclonal antibody datasheet DATASHEET Monoclonal antibody raised in mouse against acrIIA4 (anti CRISPR protein IIA4) using a recombinan... | Download |
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Western blot analysis using the Diagenode monoclonal antibody directed against acrIIA4</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with the full length recombinant acrIIA4 protein (lane 1), using the Diagenode antibody against acrIIA4 (Cat. No. C15200248), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Western blot analysis using the Diagenode monoclonal antibody directed against acrIIA4</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with the full length recombinant acrIIA4 protein (lane 1), using the Diagenode antibody against acrIIA4 (Cat. No. C15200248), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Western blot analysis using the Diagenode monoclonal antibody directed against acrIIA4</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with the full length recombinant acrIIA4 protein (lane 1), using the Diagenode antibody against acrIIA4 (Cat. No. C15200248), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Western blot analysis using the Diagenode monoclonal antibody directed against acrIIA4</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with the full length recombinant acrIIA4 protein (lane 1), using the Diagenode antibody against acrIIA4 (Cat. No. C15200248), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Western blot analysis using the Diagenode monoclonal antibody directed against acrIIA4</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with the full length recombinant acrIIA4 protein (lane 1), using the Diagenode antibody against acrIIA4 (Cat. No. C15200248), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Western blot analysis using the Diagenode monoclonal antibody directed against acrIIA4</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with the full length recombinant acrIIA4 protein (lane 1), using the Diagenode antibody against acrIIA4 (Cat. No. C15200248), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Western blot analysis using the Diagenode monoclonal antibody directed against acrIIA4</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with the full length recombinant acrIIA4 protein (lane 1), using the Diagenode antibody against acrIIA4 (Cat. No. C15200248), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Western blot analysis using the Diagenode monoclonal antibody directed against acrIIA4</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with the full length recombinant acrIIA4 protein (lane 1), using the Diagenode antibody against acrIIA4 (Cat. No. C15200248), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Western blot analysis using the Diagenode monoclonal antibody directed against acrIIA4</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with the full length recombinant acrIIA4 protein (lane 1), using the Diagenode antibody against acrIIA4 (Cat. No. C15200248), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Western blot analysis using the Diagenode monoclonal antibody directed against acrIIA4</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with the full length recombinant acrIIA4 protein (lane 1), using the Diagenode antibody against acrIIA4 (Cat. No. C15200248), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Western blot analysis using the Diagenode monoclonal antibody directed against acrIIA4</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with the full length recombinant acrIIA4 protein (lane 1), using the Diagenode antibody against acrIIA4 (Cat. No. C15200248), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Western blot analysis using the Diagenode monoclonal antibody directed against acrIIA4</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with the full length recombinant acrIIA4 protein (lane 1), using the Diagenode antibody against acrIIA4 (Cat. No. C15200248), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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es', 'language' => 'es', 'url' => 'files/SDS/acrIIA4/SDS-C15200248-acrIIA4_antibody-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2021-08-30 17:19:30', 'created' => '2021-08-30 17:19:30', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/cn/p/acrIIA4-monoclonal-antibody-50-ug' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '2995', 'antibody_id' => '545', 'name' => 'acrIIA4 Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against acrIIA4 (anti CRISPR protein IIA4) using a recombinant protein.</p>', 'label1' => 'Validation 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Western blot analysis using the Diagenode monoclonal antibody directed against acrIIA4</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with the full length recombinant acrIIA4 protein (lane 1), using the Diagenode antibody against acrIIA4 (Cat. No. C15200248), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Western blot analysis using the Diagenode monoclonal antibody directed against acrIIA4</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with the full length recombinant acrIIA4 protein (lane 1), using the Diagenode antibody against acrIIA4 (Cat. No. C15200248), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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