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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig1.png" alt="Ago (Argonautes) Antibody validated in IP" caption="false" width="278" height="164" /></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig2.png" alt="coimmunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15200167-wb.png" alt="Ago (Argonautes) Antibody validated in WB" caption="false" width="278" height="359" /></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_if.jpg" alt="Ago (Argonautes) Antibody validated in IF" caption="false" width="278" height="91" /></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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'description' => '<p>Purified monoclonal antibody prepared by immunizing mice with recombinant human Ago2 protein (GST fusion protein containing amino acids 47–879 of Ago2). The antibody also recognizes other Ago family members although with the highest affinity for Ago2.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig1.png" alt="Ago (Argonautes) Antibody validated in IP" caption="false" width="278" height="164" /></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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'description' => '<p>Purified monoclonal antibody prepared by immunizing mice with recombinant human Ago2 protein (GST fusion protein containing amino acids 47–879 of Ago2). The antibody also recognizes other Ago family members although with the highest affinity for Ago2.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig1.png" alt="Ago (Argonautes) Antibody validated in IP" caption="false" width="278" height="164" /></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig2.png" alt="coimmunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15200167-wb.png" alt="Ago (Argonautes) Antibody validated in WB" caption="false" width="278" height="359" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_if.jpg" alt="Ago (Argonautes) Antibody validated in IF" caption="false" width="278" height="91" /></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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Argonautes are needed for miRNA-induced silencing and contain two conserved RNA binding domains: a PAZ domain that can bind the single stranded 3’ end of the mature miRNA and a PIWI domain that structurally resembles ribonuclease-H and functions to interact with the 5’ end of the guide strand.
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<td>IP (RIP)</td>
<td>8 μg per IP</td>
<td>Fig 1</td>
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<td>HITS-CLIP</td>
<td>-</td>
<td>Fig 2</td>
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<td>1:500</td>
<td><span>Fig 3</span></td>
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<td>IF</td>
<td>1:500</td>
<td>Fig 4</td>
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<td>IHC</td>
<td>1:500</td>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig1.png" alt="Ago (Argonautes) Antibody validated in IP" caption="false" width="278" height="164" /></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig2.png" alt="coimmunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
</div>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15200167-wb.png" alt="Ago (Argonautes) Antibody validated in WB" caption="false" width="278" height="359" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_if.jpg" alt="Ago (Argonautes) Antibody validated in IF" caption="false" width="278" height="91" /></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig1.png" alt="Ago (Argonautes) Antibody validated in IP" caption="false" width="278" height="164" /></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig2.png" alt="coimmunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15200167-wb.png" alt="Ago (Argonautes) Antibody validated in WB" caption="false" width="278" height="359" /></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_if.jpg" alt="Ago (Argonautes) Antibody validated in IF" caption="false" width="278" height="91" /></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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'description' => '<p>Purified monoclonal antibody prepared by immunizing mice with recombinant human Ago2 protein (GST fusion protein containing amino acids 47–879 of Ago2). The antibody also recognizes other Ago family members although with the highest affinity for Ago2.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig1.png" alt="Ago (Argonautes) Antibody validated in IP" caption="false" width="278" height="164" /></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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'description' => '<p>Purified monoclonal antibody prepared by immunizing mice with recombinant human Ago2 protein (GST fusion protein containing amino acids 47–879 of Ago2). The antibody also recognizes other Ago family members although with the highest affinity for Ago2.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig1.png" alt="Ago (Argonautes) Antibody validated in IP" caption="false" width="278" height="164" /></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig2.png" alt="coimmunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15200167-wb.png" alt="Ago (Argonautes) Antibody validated in WB" caption="false" width="278" height="359" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_if.jpg" alt="Ago (Argonautes) Antibody validated in IF" caption="false" width="278" height="91" /></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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Argonautes are needed for miRNA-induced silencing and contain two conserved RNA binding domains: a PAZ domain that can bind the single stranded 3’ end of the mature miRNA and a PIWI domain that structurally resembles ribonuclease-H and functions to interact with the 5’ end of the guide strand.
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<td>IP (RIP)</td>
<td>8 μg per IP</td>
<td>Fig 1</td>
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<td>HITS-CLIP</td>
<td>-</td>
<td>Fig 2</td>
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<td>1:500</td>
<td><span>Fig 3</span></td>
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<td>IF</td>
<td>1:500</td>
<td>Fig 4</td>
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<td>IHC</td>
<td>1:500</td>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig1.png" alt="Ago (Argonautes) Antibody validated in IP" caption="false" width="278" height="164" /></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig2.png" alt="coimmunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
</div>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15200167-wb.png" alt="Ago (Argonautes) Antibody validated in WB" caption="false" width="278" height="359" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_if.jpg" alt="Ago (Argonautes) Antibody validated in IF" caption="false" width="278" height="91" /></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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×
