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<p><small><strong>Figure 1. C/EBP alpha antibody IP results</strong><br /> C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell extract A: Control with 3 μg of pre-immune rabbit IgG B: Immunoprecipitation of C/EBP alpha by 3 μg of C/EBP alpha antibody (C15410225) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (C15410225) diluted 1:1,000. </small></p>
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<p><small><strong> Figure 2. C/EBP alpha antibody ChIP results</strong><br /> ChIP was performed with A549 chromatin extract and 5 μg of either control rabbit IgG or C/EBP alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to CAMP promoter. Results are expressed as fold enrichment over the IgG negative control. </small></p>
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<p><small><strong>Figure 1. C/EBP alpha antibody IP results</strong><br /> C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell extract A: Control with 3 μg of pre-immune rabbit IgG B: Immunoprecipitation of C/EBP alpha by 3 μg of C/EBP alpha antibody (C15410225) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (C15410225) diluted 1:1,000. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><small><strong>Figure 1. C/EBP alpha antibody IP results</strong><br /> C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell extract A: Control with 3 μg of pre-immune rabbit IgG B: Immunoprecipitation of C/EBP alpha by 3 μg of C/EBP alpha antibody (C15410225) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (C15410225) diluted 1:1,000. </small></p>
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<p><small><strong> Figure 2. C/EBP alpha antibody ChIP results</strong><br /> ChIP was performed with A549 chromatin extract and 5 μg of either control rabbit IgG or C/EBP alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to CAMP promoter. Results are expressed as fold enrichment over the IgG negative control. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong>Figure 1. C/EBP alpha antibody IP results</strong><br /> C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell extract A: Control with 3 μg of pre-immune rabbit IgG B: Immunoprecipitation of C/EBP alpha by 3 μg of C/EBP alpha antibody (C15410225) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (C15410225) diluted 1:1,000. </small></p>
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<p><small><strong> Figure 2. C/EBP alpha antibody ChIP results</strong><br /> ChIP was performed with A549 chromatin extract and 5 μg of either control rabbit IgG or C/EBP alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to CAMP promoter. Results are expressed as fold enrichment over the IgG negative control. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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'name' => 'C/EBP alpha polyclonal antibody - Classic',
'description' => '<p><span>Polyclonal antibody raised in rabbit against CEBP Alpha (CCAAT/enhancer binding protein (C/EBP), alpha), using a KLH-conjugated synthetic peptide.</span></p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410225-C_EBP-polyclonal-Fig1.jpg" alt="IP result" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong>Figure 1. C/EBP alpha antibody IP results</strong><br /> C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell extract A: Control with 3 μg of pre-immune rabbit IgG B: Immunoprecipitation of C/EBP alpha by 3 μg of C/EBP alpha antibody (C15410225) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (C15410225) diluted 1:1,000. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410225-C_EBP-polyclonal-Fig2.jpg" alt="ChIP result" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 2. C/EBP alpha antibody ChIP results</strong><br /> ChIP was performed with A549 chromatin extract and 5 μg of either control rabbit IgG or C/EBP alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to CAMP promoter. Results are expressed as fold enrichment over the IgG negative control. </small></p>
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</div>',
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'format' => '25 μl',
'catalog_number' => 'C15410225-25',
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'sf_code' => 'C15410225-000032380',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '105',
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'price_GBP' => '100',
'price_JPY' => '12600',
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'slug' => 'c-ebp-alpha-polyclonal-antibody-classic-25-mg',
'meta_title' => 'C/EBP alpha Polyclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'C/EBP alpha (CCAAT/enhancer binding protein (C/EBP) Polyclonal Antibody validated in ChIP-qPCR, IP and WB. ',
'modified' => '2020-10-01 09:03:43',
'created' => '2015-06-29 14:08:20',
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'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
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'name' => 'ChIP-qPCR (ab)',
'description' => '',
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'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
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'id' => '1085',
'name' => 'Datasheet C/EBP C15410225 - Lot 40058',
'description' => '<p>Polyclonal antibody raised in rabbit against CEBP Alpha (CCAAT/enhancer binding protein (C/EBP), alpha), using a KLH-conjugated synthetic peptide.</p>',
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'type' => 'Datasheet',
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'modified' => '2020-07-01 11:00:52',
'created' => '2020-07-01 10:59:37',
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'name' => 'Modulation of vitamin D signaling by the pioneer factor CEBPA.',
'authors' => 'Nurminen V, Neme A, Seuter S, Carlberg C',
'description' => '<p>The myeloid master regulator CCAAT enhancer-binding protein alpha (CEBPA) is known as a pioneer factor. In this study, we report the CEBPA cistrome of THP-1 human monocytes after stimulation with the vitamin D receptor (VDR) ligand 1α,25-dihydroxyvitamin D (1,25(OH)D) for 2, 8 and 24 h. About a third of the genomic VDR binding sites co-located with those of CEBPA. In parallel, the binding strength of 5% of the CEBPA cistrome, i.e. some 1500 sites, is significantly (p < 0.001) affected by 1,25(OH)D. Transcriptome-wide analysis after CEBPA silencing indicated that the pioneer factor enhances both the basal expression and ligand inducibility of 70 vitamin D target genes largely involved in lipid signaling and metabolism. In contrast, CEBPA suppresses 82 vitamin D target genes many of which are related to the modulation of T cell activity by monocytes. The inducibility of the promoter-specific histone marker H3K4me3 distinguishes the former class of genes from the latter. Moreover, prominent occupancy of the myeloid pioneer factor PU.1 on 1,25(OH)D-sensitive CEBPA enhancers mechanistically explains the dichotomy of vitamin D target genes. In conclusion, CEBPA supports vitamin D signaling concerning actions of the innate immune system, but uses the antagonism with PU.1 for suppressing possible overreactions of adaptive immunity.</p>',
'date' => '2018-06-12',
'pmid' => 'http://www.pubmed.gov/30550771',
'doi' => '10.1016/j.bbagrm.2018.12.004',
'modified' => '2019-04-17 14:53:41',
'created' => '2019-04-16 12:25:30',
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
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[main] - APP/webroot/index.php, line 118
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