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<p style="text-align: justify;"><span>Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – <a href="https://www.diagenode.com/en/pages/chromatin-prep-easyshear-kit-guide">Read more</a></span></p>',
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<p style="text-align: justify;"><span>Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – <a href="https://www.diagenode.com/en/pages/chromatin-prep-easyshear-kit-guide">Read more</a></span></p>',
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<h3>Successful ChIP-seq experiments for a variety of plants</h3>
<div class="row">
<div class="small-6 columns">
<h4 class="text-center">Arabidopsis</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG1"> <img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A-small.jpg" /> </a></p>
<div id="IMG1" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></p>
<a class="close-reveal-modal" aria-label="Close">×</a></div>
<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
</div>
<div class="small-6 columns">
<h4 class="text-center">Poplar</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG2"><img src="https://www.diagenode.com/img/landing-pages/poplar-small.jpg" /> </a></p>
<div id="IMG2" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">×</a></div>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<h4 class="text-center">Tomato</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG3"> <img src="https://www.diagenode.com/img/landing-pages/tomtato-small.jpg" /> </a></p>
<div id="IMG3" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">×</a></div>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns">
<h4 class="text-center">Maize</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG4"> <img src="https://www.diagenode.com/img/landing-pages/maize-small.jpg" /> </a></p>
<div id="IMG4" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">×</a></div>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our <a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">Premium H3K27me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<p><strong> </strong></p>
<table style="width: 856px;">
<tbody>
<tr>
<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
</td>
<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
</td>
<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
</td>
</tr>
<tr>
<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
</tbody>
</table>
<p><strong></strong></p>
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'meta_title' => 'Universal Plant ChIP-seq kit | Diagenode',
'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar',
'meta_description' => 'Optimized extraction of plant chromatin from Arabidopsis,maize,rice,tomato,poplar.Complete ChIP kit including plant-specific control primer pairs and antibody',
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'description' => '<p style="text-align: justify;">The <strong>Auto Universal Plant ChIP-seq</strong> kit offers the convenience of extracting plant chromatin from a wide variety of plants including Arabidopsis, maize, rice, tomato and poplar and has been validated for the <strong>IP-Star® automated system</strong>. This complete kit has been specifically optimized for <strong>plant chromatin extraction</strong> and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.</p>',
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<li><strong>Universal compatiblity</strong> with a wide variety of plant species</li>
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<h3>Successful ChIP-seq experiments for a variety of plants</h3>
<div class="row">
<div class="small-6 columns"><center>Arabidopsis</center><center><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></center>
<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
</div>
<div class="small-6 columns"><center>Poplar</center><center><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></center>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<table style="width: 856px;">
<tbody>
<tr>
<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
</td>
<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
</td>
<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
</td>
</tr>
<tr>
<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
</tbody>
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'slug' => 'auto-universal-plant-chip-seq-kit-x24-24-rxns',
'meta_title' => 'Auto Universal Plant ChIP-seq kit | Diagenode',
'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar, automated system, automation, IP-Star',
'meta_description' => 'Plant chromatin extraction from Arabidopsis,maize,rice,tomato,poplar.Complete ChIP kit including plant-specific primer pairs,antibody.Compatible with Automation',
'modified' => '2017-08-19 10:28:51',
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'id' => '8',
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'name' => 'Chromatin shearing',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
</div>
<div class="small-12 medium-12 large-12 columns">
<div class="page" title="Page 7">
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
</div>
</div>
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'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode',
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'description' => '<p>Optimal detergent concentration is required for best results with chromatin shearing. Diagenode offers four kits with different SDS concentrations that have been pre-optimized for your specific workflow requirements. Our Chromatin EasyShear Kits (previous name: Chromatin Shearing Optimization Kits) together with the Bioruptor combine efficient cell lysis and chromatin shearing leading to consistent results.</p>
<p>The Chromatin EasyShear Kits are recommended for:</p>
<ul>
<li>The optimization of the chromatin shearing and/or chromatin preparation prior to any ChIP protocol</li>
<li>The optimization of the chromatin shearing of a new cell line/new sample type prior to ChIP using Diagenode’s ChIP kits</li>
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<p>Check all <a href="https://www.diagenode.com/en/categories/chromatin-shearing">Chromatin EasyShear Kits</a>.</p>
<p>Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – <a href="https://www.diagenode.com/en/pages/chromatin-prep-easyshear-kit-guide">Read more</a></p>
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<p><img src="https://www.diagenode.com/img/areas/plant.jpg" /></p>
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<div class="extra-spaced">
<h2>Epigenetic Regulation in Plants</h2>
<p>Plants utilize a number of gene regulation mechanisms to ensure proper development, function, growth, and survival under different environmental conditions. Plants depend on changes in gene expression to respond to environmental stimuli, in which the full repertoire of histone modifications, DNA methylation, and small ncRNAs play an important role in epigenetic regulation.</p>
<p>Studying the epigenetics of model plants such as Arabidopsis thaliana have allowed researchers to understand pathways that maintain chromatin modifications as well as the mapping of modifications such as DNA methylation on a genome-wide scale. Small RNAs have also been implicated in playing a role in the distribution of chromatin modifications, and RNA may also play a role in the complex epigenetic interactions that occur between homologous sequences (Moazed et al, 2009). In the future, by understanding epigenetic control, researchers can uncover the research necessary to improve plant growth, yields, and transformation efficiency especially in the face of climate change and other environmental factors.</p>
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<p><img src="https://www.diagenode.com/img/areas/chromatin-and-transcription-factors.jpg" /></p>
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<h3 style="font-weight: 100; margin-top: 0;">Chromatin</h3>
<p>Chromatin consists of nucleosomes formed by a complex of histone proteins and DNA, which allows the packaging of DNA into the nucleus. The less condensed euchromatin represents transcriptionally active regions, while heterochromatin is usually inactive (Vaillant and Paszkowski, 2007). Chromatin state is known to be influenced by both DNA methylation and histone modifications which in turn impact gene expression and the structure of chromosomes. In a recent study, the role of chromatin modifications during plant reproduction elucidated 3-dimensional chromosome reorganization mediated by histones and DNA methylation (Dukowic-Schulze et al. 2017). In addition, gibberellins have been shown in increasing the level of histone acetylation, which affects regions of chromatin involved in maize seed germination (Zheng et al. 2017). Another study reports a novel function of a tomato histone deacetylase gene in the regulation of fruit ripening (Guo et al. 2017).</p>
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<p><img src="https://www.diagenode.com/img/areas/cherry-tomato-common-grape-vine-ripening-fruit-vegetable-cherry-tomatoes.jpg" /></p>
</div>
<div class="small-12 medium-9 large-9 columns">
<p>In addition, multigene families encode transcription factors, with members found throughout the genome or clustered on the same chromosome. Numerous DNA binding proteins that interact with plant promoters have been identified -- some are similar to well-characterized transcription factors in animals or yeast, while others are unique to plants. For example, diverse members of the subfamily X of the plant-specific ethylene response factor (ERF) transcription factors coordinate stress signaling with wound repair activation. Tissue repair is also enhanced through a protein complex of ERF and GRAS TFs (Heyman et. al,.2018). A compilation of known plant transcription factors can be found in the plant transcription factor database at http://plntfdb.bio.uni-potsdam.de/v3.0/.</p>
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<p><img src="https://www.diagenode.com/img/areas/rna-strand.jpg" /></p>
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<h3 style="font-weight: 100; margin-top: 0;">RNA</h3>
<p>Recent research shows that a number of classes of small RNAs are key epigenetic regulators. In many cases, small RNAs have been implicated in DNA methylation and chromatin modification (Meyer, 2015). In addition, the role of small RNAs has been implicated in plant stress tolerance (Kumar et al., 2017). López-Galiano et al also provided insight into a coordinated function of a miRNA gene and histone modifications in regulating the expression of a WRKY transcription factor in response to stress.</p>
<p>RNA interference (RNAi) is another epigenetic mechanism that leads to small RNA generation, which mediates gene silencing at the post-transcriptional level. RNAi technology has immense potential for plant disease resistance.</p>
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<p><img src="https://www.diagenode.com/img/areas/dna-methylation.jpg" /></p>
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<h3 style="font-weight: 100; margin-top: 0;">DNA methylation</h3>
<p>Plants, unlike animals, have three sites that can be methylated G, CHG (H can be A, C, T), and CHH (Law and Jacobsen, 2010). DNA methylation has attracted particular interest. In Arabidopsis, one-third of methylated genes occur in transcribed regions, and 5% of genes are methylated in promoter regions, suggesting that many of these are epigenetically regulated. (Zhang et al., 2006).</p>
<p>There are thousands of differentially methylated regions (DMRs) that influence phenotype by influencing gene expression. The analysis of epigenetic recombinant inbred line (epiRIL) plants from Arabidopsis points to the evidence of the influence of DMRs. An epiRIL results from crossing two genetically identical plants with differing DNA methylation levels (with one parent as a homozygous mutant for an essential DNA methylation maintenance gene). The offspring of these plants have similar genomes that vary only in methylation levels. Many traits have been studied using epiRILs -- flowering time, plant height, and response to abiotic stress, some of which have now been mapped to DMRs (Zhang et al. 2018)</p>
<p>Regulation by DNA methylation has been shown to be important in many aspects of plant development and response such as vernalization, hybrid vigor, and self-incompatibility (Itabashi et al. 2017). For example, vernalization treatments have shown reduced DNA methylation and subsequent initiation of flowering (Burn et al., 1993). Stress can also influence DNA methylation in plants as a response to environmental stimuli. (Steward et al., 2002; Song et al., 2012). A high degree of DNA methylation has also suggested the role in the improvement of plant fitness under different environmental conditions (Saéz-Laguna et al., 2014). In addition, methylation can affect normal fruit and hypomethylation predicts homeotic transformation and loss of fruit yield (Ong-Abdullah et al., 2015)</p>
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<p>DNA demethylation has also been implied in various aspects of plant development including pollen tube formation, embryogenesis, fruit ripening, stomatal development, and nodule formation ( Li et al. 2017). Demethylation of rice genomic DNA caused an altered pattern of gene expression, inducing dwarf plants (Sano et al., 1990).</p>
<p>Epigenetic modifications contribute to the stability and survival of the plants and their ability to adapt in different environmental conditions.</p>
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<h3>Diagenode products for your epigenomics research in plants</h3>
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<h3 class="text-center"><a href="https://www.diagenode.com/en/categories/chromatin-function">Chromatin analysis</a></h3>
<center><a href="https://www.diagenode.com/en/categories/chromatin-function"><img src="https://www.diagenode.com/img/cancer/chromatin-icon.png" /></a></center>
<p class="text-left">Understand the role of chromatin in plant function and development</p>
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<li><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns"> Learn about the Universal Plant ChIP Kit</a></li>
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<h3 class="text-center"><a href="https://www.diagenode.com/en/categories/dna-methylation" style="color: #30415c;">DNA methylation</a></h3>
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<p class="text-left">DNA methylation and demethylation and the effects on plant response and function</p>
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<h3 class="text-center"><span class="darkgrey">Non-coding RNAs</span></h3>
<center><img src="https://www.diagenode.com/img/cancer/non-coding-icon.png" /></center>
<p class="text-left">Discover noncoding RNAs in the regulation of gene expression in plants</p>
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<li><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">Library prep for RNA-seq studies for ncRNAs</a></li>
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<h3>References</h3>
<p><small> Burn, J. et al (1993). DNA methylation, vernalization, and the initiation of flowering. Proc. Natl. Acad. Sci. U.S.A. 90, 287–291. doi: 10.1006/scdb.1996.0055 </small></p>
<p><small> Dukowic-Schulze S, Liu C, Chen C (2017) Not just gene expression: 3D implications of chromatin modifications during sexual plant reproduction. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2222-0</small></p>
<p><small> Guo J et al (2017) A histone deacetylase gene, SlHDA3, acts as a negative regulator of fruit ripening and carotenoid accumulation. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2211-3</small></p>
<p><small> Heyman J, et.al (2018) Journal of Cell Science Emerging role of the plant ERF transcription factors in coordinating wound defense responses and repair doi: 10.1242/jcs.208215</small></p>
<p><small> Itabashi E, Osabe K, Fujimoto R, Kakizaki T (2017) Epigenetic regulation of agronomical traits in Brassicaceae. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2223-z</small></p>
<p><small> Kumar V et al (2017) Plant small RNAs: the essential epigenetic regulators of gene expression for salt-stress responses and tolerance. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2210-4</small></p>
<p><small> Law, J. A., and Jacobsen, S. E. (2010). Establishing, maintaining and modifying DNA methylation patterns in plants and animals. Nat. Rev. Genet. 11, 204–220. doi: 10.1038/nrg2719</small></p>
<p><small> Meyer, P. (2015). Epigenetic variation and environmental change. J. Exp. Bot. 66, 3541–3548. doi: 10.1093/jxb/eru502</small></p>
<p><small> Moazed, D. (2009) Small RNAs in transcriptional gene silencing and genome defence. Nature. doi: 10.1038/nature07756</small></p>
<p><small> Ong-Abdullah et al. (2015). Loss of Karma transposon methylation underlies the mantled somaclonal variant of oil palm. Nature 525, 533–537. doi: 10.1038/nature15365</small></p>
<p><small> Saéz-Laguna et al. (2014). Epigenetic variability in the genetically uniform forest tree species. PLoS One 9:e103145. doi: 10.1371/journal.pone.0103145</small></p>
<p><small> Sano, H. et al. (1990). A single treatment of rice seedlings with 5-azacytidine induces heritable dwarfism and undermethylation of genomic DNA. Mol. Gen. Genet. 220, 441–447. doi: 10.1007/BF00391751</small></p>
<p><small> Song, J et al (2012). Vernalization – A cold-induced epigenetic switch. J. Cell Sci. 125, 3723–3731. doi: 10.1242/jcs.084764</small></p>
<p><small> Steward, N et al. (2002). Periodic DNA methylation in maize nucleosomes and demethylation by environmental stress. J. Biol. Chem. 277, 37741–37746. doi: 10.1074/jbc.M204050200</small></p>
<p><small> Vaillant, I., and Paszkowski, J. (2007). Role of histone and DNA methylation in gene regulation. Curr. Opin. Plant Biol. 10, 528–533. doi: 10.1016/j.pbi.2007.06.008</small></p>
<p><small> Zhang, et al. (2006). Genome-wide high-resolution mapping and functional analysis of DNA methylation in Arabidopsis. Cell 126, 1189–1201. doi: 10.1016/j.cell.2006.08.003</small></p>
<p><small> Zhang et al. 2018 Understanding the evolutionary potential of epigenetic variation: a comparison of heritable phenotypic variation in epiRILs, RILs, and natural ecotypes of Arabidopsis thaliana. Heredity 121, 257–265 (2018) doi:10.1038/s41437-018-0095-9</small></p>
<p><small> Zheng X et al (2017) Histone acetylation is involved in GA-mediated 45S rDNA decondensation in maize aleurone layers. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2207-z</small></p>
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<h6 style="height:60px">Auto Universal Plant ChIP-seq kit</h6>
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<h3>Successful ChIP-seq experiments for a variety of plants</h3>
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<div class="small-6 columns"><center>Arabidopsis</center><center><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></center>
<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
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<div class="small-6 columns"><center>Poplar</center><center><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></center>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
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<div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
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<div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
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<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
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<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
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<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
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<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
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<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
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<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
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<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
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<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
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<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
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<td style="width: 224px;"></td>
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<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
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<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
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<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
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<p style="text-align: justify;"><span>The first critical step of a successful ChIP experiment is the best preparation of sheared chromatin. This <strong>Chromatin Shearing Optimization Kit</strong> is designed to be used in conjunction with the <strong>Universal Plant ChIP-seq kit</strong> and contains the right level of <strong>detergent</strong> for extraction of highest quality plant chromatin for ChIP. In addition, the signature</span><span> crosslinking containers of this kit provide a simple and reliable method for fixation. The content of this kit is enough to perform 12 chromatin extractions.<br /></span></p>
<p style="text-align: justify;"><span>Check all <a href="https://www.diagenode.com/en/categories/chromatin-shearing">Chromatin EasyShear Kits</a>.</span></p>
<p style="text-align: justify;"><span>Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – <a href="https://www.diagenode.com/en/pages/chromatin-prep-easyshear-kit-guide">Read more</a></span></p>',
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<p style="text-align: justify;">The <strong>Universal Plant ChIP-seq kit</strong> offers the convenience of extracting plant chromatin from a wide variety of plants including Arabidopsis, maize, rice, tomato and poplar. This complete kit has been specifically optimized for <strong>plant chromatin extraction</strong> and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.</p>',
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<h3>Successful ChIP-seq experiments for a variety of plants</h3>
<div class="row">
<div class="small-6 columns">
<h4 class="text-center">Arabidopsis</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG1"> <img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A-small.jpg" /> </a></p>
<div id="IMG1" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></p>
<a class="close-reveal-modal" aria-label="Close">×</a></div>
<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
</div>
<div class="small-6 columns">
<h4 class="text-center">Poplar</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG2"><img src="https://www.diagenode.com/img/landing-pages/poplar-small.jpg" /> </a></p>
<div id="IMG2" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">×</a></div>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<h4 class="text-center">Tomato</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG3"> <img src="https://www.diagenode.com/img/landing-pages/tomtato-small.jpg" /> </a></p>
<div id="IMG3" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">×</a></div>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns">
<h4 class="text-center">Maize</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG4"> <img src="https://www.diagenode.com/img/landing-pages/maize-small.jpg" /> </a></p>
<div id="IMG4" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">×</a></div>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our <a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">Premium H3K27me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<p><strong> </strong></p>
<table style="width: 856px;">
<tbody>
<tr>
<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
</td>
<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
</td>
<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
</td>
</tr>
<tr>
<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
</tbody>
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<h3>Successful ChIP-seq experiments for a variety of plants</h3>
<div class="row">
<div class="small-6 columns"><center>Arabidopsis</center><center><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></center>
<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
</div>
<div class="small-6 columns"><center>Poplar</center><center><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></center>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<table style="width: 856px;">
<tbody>
<tr>
<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
</td>
<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
</td>
<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
</td>
</tr>
<tr>
<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
</tbody>
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'meta_title' => 'Auto Universal Plant ChIP-seq kit | Diagenode',
'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar, automated system, automation, IP-Star',
'meta_description' => 'Plant chromatin extraction from Arabidopsis,maize,rice,tomato,poplar.Complete ChIP kit including plant-specific primer pairs,antibody.Compatible with Automation',
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<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
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<div class="small-12 medium-12 large-12 columns">
<div class="page" title="Page 7">
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
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<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
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<li>The optimization of the chromatin shearing and/or chromatin preparation prior to any ChIP protocol</li>
<li>The optimization of the chromatin shearing of a new cell line/new sample type prior to ChIP using Diagenode’s ChIP kits</li>
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<p>Check all <a href="https://www.diagenode.com/en/categories/chromatin-shearing">Chromatin EasyShear Kits</a>.</p>
<p>Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – <a href="https://www.diagenode.com/en/pages/chromatin-prep-easyshear-kit-guide">Read more</a></p>
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<p><img src="https://www.diagenode.com/img/areas/plant.jpg" /></p>
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<div class="extra-spaced">
<h2>Epigenetic Regulation in Plants</h2>
<p>Plants utilize a number of gene regulation mechanisms to ensure proper development, function, growth, and survival under different environmental conditions. Plants depend on changes in gene expression to respond to environmental stimuli, in which the full repertoire of histone modifications, DNA methylation, and small ncRNAs play an important role in epigenetic regulation.</p>
<p>Studying the epigenetics of model plants such as Arabidopsis thaliana have allowed researchers to understand pathways that maintain chromatin modifications as well as the mapping of modifications such as DNA methylation on a genome-wide scale. Small RNAs have also been implicated in playing a role in the distribution of chromatin modifications, and RNA may also play a role in the complex epigenetic interactions that occur between homologous sequences (Moazed et al, 2009). In the future, by understanding epigenetic control, researchers can uncover the research necessary to improve plant growth, yields, and transformation efficiency especially in the face of climate change and other environmental factors.</p>
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<div class="row extra-spaced">
<div class="small-12 medium-3 large-3 columns">
<p><img src="https://www.diagenode.com/img/areas/chromatin-and-transcription-factors.jpg" /></p>
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<div class="small-12 medium-9 large-9 columns">
<h3 style="font-weight: 100; margin-top: 0;">Chromatin</h3>
<p>Chromatin consists of nucleosomes formed by a complex of histone proteins and DNA, which allows the packaging of DNA into the nucleus. The less condensed euchromatin represents transcriptionally active regions, while heterochromatin is usually inactive (Vaillant and Paszkowski, 2007). Chromatin state is known to be influenced by both DNA methylation and histone modifications which in turn impact gene expression and the structure of chromosomes. In a recent study, the role of chromatin modifications during plant reproduction elucidated 3-dimensional chromosome reorganization mediated by histones and DNA methylation (Dukowic-Schulze et al. 2017). In addition, gibberellins have been shown in increasing the level of histone acetylation, which affects regions of chromatin involved in maize seed germination (Zheng et al. 2017). Another study reports a novel function of a tomato histone deacetylase gene in the regulation of fruit ripening (Guo et al. 2017).</p>
</div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-3 large-3 columns">
<p><img src="https://www.diagenode.com/img/areas/cherry-tomato-common-grape-vine-ripening-fruit-vegetable-cherry-tomatoes.jpg" /></p>
</div>
<div class="small-12 medium-9 large-9 columns">
<p>In addition, multigene families encode transcription factors, with members found throughout the genome or clustered on the same chromosome. Numerous DNA binding proteins that interact with plant promoters have been identified -- some are similar to well-characterized transcription factors in animals or yeast, while others are unique to plants. For example, diverse members of the subfamily X of the plant-specific ethylene response factor (ERF) transcription factors coordinate stress signaling with wound repair activation. Tissue repair is also enhanced through a protein complex of ERF and GRAS TFs (Heyman et. al,.2018). A compilation of known plant transcription factors can be found in the plant transcription factor database at http://plntfdb.bio.uni-potsdam.de/v3.0/.</p>
</div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-3 large-3 columns">
<p><img src="https://www.diagenode.com/img/areas/rna-strand.jpg" /></p>
</div>
<div class="small-12 medium-9 large-9 columns">
<h3 style="font-weight: 100; margin-top: 0;">RNA</h3>
<p>Recent research shows that a number of classes of small RNAs are key epigenetic regulators. In many cases, small RNAs have been implicated in DNA methylation and chromatin modification (Meyer, 2015). In addition, the role of small RNAs has been implicated in plant stress tolerance (Kumar et al., 2017). López-Galiano et al also provided insight into a coordinated function of a miRNA gene and histone modifications in regulating the expression of a WRKY transcription factor in response to stress.</p>
<p>RNA interference (RNAi) is another epigenetic mechanism that leads to small RNA generation, which mediates gene silencing at the post-transcriptional level. RNAi technology has immense potential for plant disease resistance.</p>
</div>
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<h3 style="font-weight: 100; margin-top: 0;">DNA methylation</h3>
<p>Plants, unlike animals, have three sites that can be methylated G, CHG (H can be A, C, T), and CHH (Law and Jacobsen, 2010). DNA methylation has attracted particular interest. In Arabidopsis, one-third of methylated genes occur in transcribed regions, and 5% of genes are methylated in promoter regions, suggesting that many of these are epigenetically regulated. (Zhang et al., 2006).</p>
<p>There are thousands of differentially methylated regions (DMRs) that influence phenotype by influencing gene expression. The analysis of epigenetic recombinant inbred line (epiRIL) plants from Arabidopsis points to the evidence of the influence of DMRs. An epiRIL results from crossing two genetically identical plants with differing DNA methylation levels (with one parent as a homozygous mutant for an essential DNA methylation maintenance gene). The offspring of these plants have similar genomes that vary only in methylation levels. Many traits have been studied using epiRILs -- flowering time, plant height, and response to abiotic stress, some of which have now been mapped to DMRs (Zhang et al. 2018)</p>
<p>Regulation by DNA methylation has been shown to be important in many aspects of plant development and response such as vernalization, hybrid vigor, and self-incompatibility (Itabashi et al. 2017). For example, vernalization treatments have shown reduced DNA methylation and subsequent initiation of flowering (Burn et al., 1993). Stress can also influence DNA methylation in plants as a response to environmental stimuli. (Steward et al., 2002; Song et al., 2012). A high degree of DNA methylation has also suggested the role in the improvement of plant fitness under different environmental conditions (Saéz-Laguna et al., 2014). In addition, methylation can affect normal fruit and hypomethylation predicts homeotic transformation and loss of fruit yield (Ong-Abdullah et al., 2015)</p>
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<p>DNA demethylation has also been implied in various aspects of plant development including pollen tube formation, embryogenesis, fruit ripening, stomatal development, and nodule formation ( Li et al. 2017). Demethylation of rice genomic DNA caused an altered pattern of gene expression, inducing dwarf plants (Sano et al., 1990).</p>
<p>Epigenetic modifications contribute to the stability and survival of the plants and their ability to adapt in different environmental conditions.</p>
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<h3>Diagenode products for your epigenomics research in plants</h3>
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<h3 class="text-center"><a href="https://www.diagenode.com/en/categories/chromatin-function">Chromatin analysis</a></h3>
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<p class="text-left">Understand the role of chromatin in plant function and development</p>
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<h3 class="text-center"><a href="https://www.diagenode.com/en/categories/dna-methylation" style="color: #30415c;">DNA methylation</a></h3>
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<p class="text-left">DNA methylation and demethylation and the effects on plant response and function</p>
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<h3 class="text-center"><span class="darkgrey">Non-coding RNAs</span></h3>
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<p class="text-left">Discover noncoding RNAs in the regulation of gene expression in plants</p>
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<h3>References</h3>
<p><small> Burn, J. et al (1993). DNA methylation, vernalization, and the initiation of flowering. Proc. Natl. Acad. Sci. U.S.A. 90, 287–291. doi: 10.1006/scdb.1996.0055 </small></p>
<p><small> Dukowic-Schulze S, Liu C, Chen C (2017) Not just gene expression: 3D implications of chromatin modifications during sexual plant reproduction. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2222-0</small></p>
<p><small> Guo J et al (2017) A histone deacetylase gene, SlHDA3, acts as a negative regulator of fruit ripening and carotenoid accumulation. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2211-3</small></p>
<p><small> Heyman J, et.al (2018) Journal of Cell Science Emerging role of the plant ERF transcription factors in coordinating wound defense responses and repair doi: 10.1242/jcs.208215</small></p>
<p><small> Itabashi E, Osabe K, Fujimoto R, Kakizaki T (2017) Epigenetic regulation of agronomical traits in Brassicaceae. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2223-z</small></p>
<p><small> Kumar V et al (2017) Plant small RNAs: the essential epigenetic regulators of gene expression for salt-stress responses and tolerance. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2210-4</small></p>
<p><small> Law, J. A., and Jacobsen, S. E. (2010). Establishing, maintaining and modifying DNA methylation patterns in plants and animals. Nat. Rev. Genet. 11, 204–220. doi: 10.1038/nrg2719</small></p>
<p><small> Meyer, P. (2015). Epigenetic variation and environmental change. J. Exp. Bot. 66, 3541–3548. doi: 10.1093/jxb/eru502</small></p>
<p><small> Moazed, D. (2009) Small RNAs in transcriptional gene silencing and genome defence. Nature. doi: 10.1038/nature07756</small></p>
<p><small> Ong-Abdullah et al. (2015). Loss of Karma transposon methylation underlies the mantled somaclonal variant of oil palm. Nature 525, 533–537. doi: 10.1038/nature15365</small></p>
<p><small> Saéz-Laguna et al. (2014). Epigenetic variability in the genetically uniform forest tree species. PLoS One 9:e103145. doi: 10.1371/journal.pone.0103145</small></p>
<p><small> Sano, H. et al. (1990). A single treatment of rice seedlings with 5-azacytidine induces heritable dwarfism and undermethylation of genomic DNA. Mol. Gen. Genet. 220, 441–447. doi: 10.1007/BF00391751</small></p>
<p><small> Song, J et al (2012). Vernalization – A cold-induced epigenetic switch. J. Cell Sci. 125, 3723–3731. doi: 10.1242/jcs.084764</small></p>
<p><small> Steward, N et al. (2002). Periodic DNA methylation in maize nucleosomes and demethylation by environmental stress. J. Biol. Chem. 277, 37741–37746. doi: 10.1074/jbc.M204050200</small></p>
<p><small> Vaillant, I., and Paszkowski, J. (2007). Role of histone and DNA methylation in gene regulation. Curr. Opin. Plant Biol. 10, 528–533. doi: 10.1016/j.pbi.2007.06.008</small></p>
<p><small> Zhang, et al. (2006). Genome-wide high-resolution mapping and functional analysis of DNA methylation in Arabidopsis. Cell 126, 1189–1201. doi: 10.1016/j.cell.2006.08.003</small></p>
<p><small> Zhang et al. 2018 Understanding the evolutionary potential of epigenetic variation: a comparison of heritable phenotypic variation in epiRILs, RILs, and natural ecotypes of Arabidopsis thaliana. Heredity 121, 257–265 (2018) doi:10.1038/s41437-018-0095-9</small></p>
<p><small> Zheng X et al (2017) Histone acetylation is involved in GA-mediated 45S rDNA decondensation in maize aleurone layers. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2207-z</small></p>
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<h3>Successful ChIP-seq experiments for a variety of plants</h3>
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<div class="small-6 columns"><center>Arabidopsis</center><center><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></center>
<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
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<div class="small-6 columns"><center>Poplar</center><center><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></center>
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<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
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<div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
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<h4><strong>Plant Species</strong></h4>
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<h4><strong>Validated primer pairs</strong></h4>
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<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
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<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
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<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
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<td style="width: 224px;"></td>
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<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
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<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
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<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
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<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
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<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
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<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
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<tr>
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<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
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<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
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<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
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<h4 class="text-center">Arabidopsis</h4>
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<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></p>
<a class="close-reveal-modal" aria-label="Close">×</a></div>
<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
</div>
<div class="small-6 columns">
<h4 class="text-center">Poplar</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG2"><img src="https://www.diagenode.com/img/landing-pages/poplar-small.jpg" /> </a></p>
<div id="IMG2" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">×</a></div>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<h4 class="text-center">Tomato</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG3"> <img src="https://www.diagenode.com/img/landing-pages/tomtato-small.jpg" /> </a></p>
<div id="IMG3" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">×</a></div>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns">
<h4 class="text-center">Maize</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG4"> <img src="https://www.diagenode.com/img/landing-pages/maize-small.jpg" /> </a></p>
<div id="IMG4" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">×</a></div>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our <a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">Premium H3K27me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<p><strong> </strong></p>
<table style="width: 856px;">
<tbody>
<tr>
<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
</td>
<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
</td>
<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
</td>
</tr>
<tr>
<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
</tbody>
</table>
<p><strong></strong></p>
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'slug' => 'universal-plant-chip-seq-kit-x24-24-rxns',
'meta_title' => 'Universal Plant ChIP-seq kit | Diagenode',
'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar',
'meta_description' => 'Optimized extraction of plant chromatin from Arabidopsis,maize,rice,tomato,poplar.Complete ChIP kit including plant-specific control primer pairs and antibody',
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'description' => '<p style="text-align: justify;">The <strong>Auto Universal Plant ChIP-seq</strong> kit offers the convenience of extracting plant chromatin from a wide variety of plants including Arabidopsis, maize, rice, tomato and poplar and has been validated for the <strong>IP-Star® automated system</strong>. This complete kit has been specifically optimized for <strong>plant chromatin extraction</strong> and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.</p>',
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<ul>
<li><strong>Universal compatiblity</strong> with a wide variety of plant species</li>
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<li>Validated for the high throughput <strong>IP-Star® Automated System</strong></li>
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<h3>Successful ChIP-seq experiments for a variety of plants</h3>
<div class="row">
<div class="small-6 columns"><center>Arabidopsis</center><center><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></center>
<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
</div>
<div class="small-6 columns"><center>Poplar</center><center><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></center>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<table style="width: 856px;">
<tbody>
<tr>
<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
</td>
<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
</td>
<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
</td>
</tr>
<tr>
<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
</tbody>
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'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar, automated system, automation, IP-Star',
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<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
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<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
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<div class="small-12 medium-12 large-12 columns">
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<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
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<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
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<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
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<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
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<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
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<li>The optimization of the chromatin shearing of a new cell line/new sample type prior to ChIP using Diagenode’s ChIP kits</li>
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<p>Check all <a href="https://www.diagenode.com/en/categories/chromatin-shearing">Chromatin EasyShear Kits</a>.</p>
<p>Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – <a href="https://www.diagenode.com/en/pages/chromatin-prep-easyshear-kit-guide">Read more</a></p>
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<p><img src="https://www.diagenode.com/img/areas/plant.jpg" /></p>
</div>
<div class="extra-spaced">
<h2>Epigenetic Regulation in Plants</h2>
<p>Plants utilize a number of gene regulation mechanisms to ensure proper development, function, growth, and survival under different environmental conditions. Plants depend on changes in gene expression to respond to environmental stimuli, in which the full repertoire of histone modifications, DNA methylation, and small ncRNAs play an important role in epigenetic regulation.</p>
<p>Studying the epigenetics of model plants such as Arabidopsis thaliana have allowed researchers to understand pathways that maintain chromatin modifications as well as the mapping of modifications such as DNA methylation on a genome-wide scale. Small RNAs have also been implicated in playing a role in the distribution of chromatin modifications, and RNA may also play a role in the complex epigenetic interactions that occur between homologous sequences (Moazed et al, 2009). In the future, by understanding epigenetic control, researchers can uncover the research necessary to improve plant growth, yields, and transformation efficiency especially in the face of climate change and other environmental factors.</p>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-3 large-3 columns">
<p><img src="https://www.diagenode.com/img/areas/chromatin-and-transcription-factors.jpg" /></p>
</div>
<div class="small-12 medium-9 large-9 columns">
<h3 style="font-weight: 100; margin-top: 0;">Chromatin</h3>
<p>Chromatin consists of nucleosomes formed by a complex of histone proteins and DNA, which allows the packaging of DNA into the nucleus. The less condensed euchromatin represents transcriptionally active regions, while heterochromatin is usually inactive (Vaillant and Paszkowski, 2007). Chromatin state is known to be influenced by both DNA methylation and histone modifications which in turn impact gene expression and the structure of chromosomes. In a recent study, the role of chromatin modifications during plant reproduction elucidated 3-dimensional chromosome reorganization mediated by histones and DNA methylation (Dukowic-Schulze et al. 2017). In addition, gibberellins have been shown in increasing the level of histone acetylation, which affects regions of chromatin involved in maize seed germination (Zheng et al. 2017). Another study reports a novel function of a tomato histone deacetylase gene in the regulation of fruit ripening (Guo et al. 2017).</p>
</div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-3 large-3 columns">
<p><img src="https://www.diagenode.com/img/areas/cherry-tomato-common-grape-vine-ripening-fruit-vegetable-cherry-tomatoes.jpg" /></p>
</div>
<div class="small-12 medium-9 large-9 columns">
<p>In addition, multigene families encode transcription factors, with members found throughout the genome or clustered on the same chromosome. Numerous DNA binding proteins that interact with plant promoters have been identified -- some are similar to well-characterized transcription factors in animals or yeast, while others are unique to plants. For example, diverse members of the subfamily X of the plant-specific ethylene response factor (ERF) transcription factors coordinate stress signaling with wound repair activation. Tissue repair is also enhanced through a protein complex of ERF and GRAS TFs (Heyman et. al,.2018). A compilation of known plant transcription factors can be found in the plant transcription factor database at http://plntfdb.bio.uni-potsdam.de/v3.0/.</p>
</div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-3 large-3 columns">
<p><img src="https://www.diagenode.com/img/areas/rna-strand.jpg" /></p>
</div>
<div class="small-12 medium-9 large-9 columns">
<h3 style="font-weight: 100; margin-top: 0;">RNA</h3>
<p>Recent research shows that a number of classes of small RNAs are key epigenetic regulators. In many cases, small RNAs have been implicated in DNA methylation and chromatin modification (Meyer, 2015). In addition, the role of small RNAs has been implicated in plant stress tolerance (Kumar et al., 2017). López-Galiano et al also provided insight into a coordinated function of a miRNA gene and histone modifications in regulating the expression of a WRKY transcription factor in response to stress.</p>
<p>RNA interference (RNAi) is another epigenetic mechanism that leads to small RNA generation, which mediates gene silencing at the post-transcriptional level. RNAi technology has immense potential for plant disease resistance.</p>
</div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-3 large-3 columns">
<p><img src="https://www.diagenode.com/img/areas/dna-methylation.jpg" /></p>
</div>
<div class="small-12 medium-9 large-9 columns">
<h3 style="font-weight: 100; margin-top: 0;">DNA methylation</h3>
<p>Plants, unlike animals, have three sites that can be methylated G, CHG (H can be A, C, T), and CHH (Law and Jacobsen, 2010). DNA methylation has attracted particular interest. In Arabidopsis, one-third of methylated genes occur in transcribed regions, and 5% of genes are methylated in promoter regions, suggesting that many of these are epigenetically regulated. (Zhang et al., 2006).</p>
<p>There are thousands of differentially methylated regions (DMRs) that influence phenotype by influencing gene expression. The analysis of epigenetic recombinant inbred line (epiRIL) plants from Arabidopsis points to the evidence of the influence of DMRs. An epiRIL results from crossing two genetically identical plants with differing DNA methylation levels (with one parent as a homozygous mutant for an essential DNA methylation maintenance gene). The offspring of these plants have similar genomes that vary only in methylation levels. Many traits have been studied using epiRILs -- flowering time, plant height, and response to abiotic stress, some of which have now been mapped to DMRs (Zhang et al. 2018)</p>
<p>Regulation by DNA methylation has been shown to be important in many aspects of plant development and response such as vernalization, hybrid vigor, and self-incompatibility (Itabashi et al. 2017). For example, vernalization treatments have shown reduced DNA methylation and subsequent initiation of flowering (Burn et al., 1993). Stress can also influence DNA methylation in plants as a response to environmental stimuli. (Steward et al., 2002; Song et al., 2012). A high degree of DNA methylation has also suggested the role in the improvement of plant fitness under different environmental conditions (Saéz-Laguna et al., 2014). In addition, methylation can affect normal fruit and hypomethylation predicts homeotic transformation and loss of fruit yield (Ong-Abdullah et al., 2015)</p>
</div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-3 large-3 columns">
<p><img src="https://www.diagenode.com/img/areas/plant-development.jpg" class="left" style="padding-right: 15px;" /></p>
</div>
<div class="small-12 medium-9 large-9 columns">
<p>DNA demethylation has also been implied in various aspects of plant development including pollen tube formation, embryogenesis, fruit ripening, stomatal development, and nodule formation ( Li et al. 2017). Demethylation of rice genomic DNA caused an altered pattern of gene expression, inducing dwarf plants (Sano et al., 1990).</p>
<p>Epigenetic modifications contribute to the stability and survival of the plants and their ability to adapt in different environmental conditions.</p>
</div>
</div>
<h3>Diagenode products for your epigenomics research in plants</h3>
<div class="row extra-spaced">
<div class="small-12 medium-4 large-4 columns text-left">
<div class="panel" style="border-color: #099f92; height: 275px;">
<h3 class="text-center"><a href="https://www.diagenode.com/en/categories/chromatin-function">Chromatin analysis</a></h3>
<center><a href="https://www.diagenode.com/en/categories/chromatin-function"><img src="https://www.diagenode.com/img/cancer/chromatin-icon.png" /></a></center>
<p class="text-left">Understand the role of chromatin in plant function and development</p>
</div>
<ul>
<li><a href="https://www.diagenode.com/en/categories/chromatin-function">Learn about our chromatin analysis products</a></li>
<li><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns"> Learn about the Universal Plant ChIP Kit</a></li>
</ul>
</div>
<div class="small-12 medium-4 large-4 columns text-left">
<div class="panel" style="border-color: #30415c; height: 275px;">
<h3 class="text-center"><a href="https://www.diagenode.com/en/categories/dna-methylation" style="color: #30415c;">DNA methylation</a></h3>
<center><a href="https://www.diagenode.com/en/categories/dna-methylation"><img src="https://www.diagenode.com/img/cancer/dna-icon.png" /></a></center>
<p class="text-left">DNA methylation and demethylation and the effects on plant response and function</p>
</div>
<ul>
<li><a href="https://www.diagenode.com/en/categories/dna-methylation">Discover DNA methylation analysis solutions at any resolution</a></li>
</ul>
</div>
<div class="small-12 medium-4 large-4 columns text-left">
<div class="panel" style="border-color: #474546; height: 275px;">
<h3 class="text-center"><span class="darkgrey">Non-coding RNAs</span></h3>
<center><img src="https://www.diagenode.com/img/cancer/non-coding-icon.png" /></center>
<p class="text-left">Discover noncoding RNAs in the regulation of gene expression in plants</p>
</div>
<ul>
<li><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">Library prep for RNA-seq studies for ncRNAs</a></li>
</ul>
</div>
</div>
<h3>References</h3>
<p><small> Burn, J. et al (1993). DNA methylation, vernalization, and the initiation of flowering. Proc. Natl. Acad. Sci. U.S.A. 90, 287–291. doi: 10.1006/scdb.1996.0055 </small></p>
<p><small> Dukowic-Schulze S, Liu C, Chen C (2017) Not just gene expression: 3D implications of chromatin modifications during sexual plant reproduction. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2222-0</small></p>
<p><small> Guo J et al (2017) A histone deacetylase gene, SlHDA3, acts as a negative regulator of fruit ripening and carotenoid accumulation. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2211-3</small></p>
<p><small> Heyman J, et.al (2018) Journal of Cell Science Emerging role of the plant ERF transcription factors in coordinating wound defense responses and repair doi: 10.1242/jcs.208215</small></p>
<p><small> Itabashi E, Osabe K, Fujimoto R, Kakizaki T (2017) Epigenetic regulation of agronomical traits in Brassicaceae. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2223-z</small></p>
<p><small> Kumar V et al (2017) Plant small RNAs: the essential epigenetic regulators of gene expression for salt-stress responses and tolerance. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2210-4</small></p>
<p><small> Law, J. A., and Jacobsen, S. E. (2010). Establishing, maintaining and modifying DNA methylation patterns in plants and animals. Nat. Rev. Genet. 11, 204–220. doi: 10.1038/nrg2719</small></p>
<p><small> Meyer, P. (2015). Epigenetic variation and environmental change. J. Exp. Bot. 66, 3541–3548. doi: 10.1093/jxb/eru502</small></p>
<p><small> Moazed, D. (2009) Small RNAs in transcriptional gene silencing and genome defence. Nature. doi: 10.1038/nature07756</small></p>
<p><small> Ong-Abdullah et al. (2015). Loss of Karma transposon methylation underlies the mantled somaclonal variant of oil palm. Nature 525, 533–537. doi: 10.1038/nature15365</small></p>
<p><small> Saéz-Laguna et al. (2014). Epigenetic variability in the genetically uniform forest tree species. PLoS One 9:e103145. doi: 10.1371/journal.pone.0103145</small></p>
<p><small> Sano, H. et al. (1990). A single treatment of rice seedlings with 5-azacytidine induces heritable dwarfism and undermethylation of genomic DNA. Mol. Gen. Genet. 220, 441–447. doi: 10.1007/BF00391751</small></p>
<p><small> Song, J et al (2012). Vernalization – A cold-induced epigenetic switch. J. Cell Sci. 125, 3723–3731. doi: 10.1242/jcs.084764</small></p>
<p><small> Steward, N et al. (2002). Periodic DNA methylation in maize nucleosomes and demethylation by environmental stress. J. Biol. Chem. 277, 37741–37746. doi: 10.1074/jbc.M204050200</small></p>
<p><small> Vaillant, I., and Paszkowski, J. (2007). Role of histone and DNA methylation in gene regulation. Curr. Opin. Plant Biol. 10, 528–533. doi: 10.1016/j.pbi.2007.06.008</small></p>
<p><small> Zhang, et al. (2006). Genome-wide high-resolution mapping and functional analysis of DNA methylation in Arabidopsis. Cell 126, 1189–1201. doi: 10.1016/j.cell.2006.08.003</small></p>
<p><small> Zhang et al. 2018 Understanding the evolutionary potential of epigenetic variation: a comparison of heritable phenotypic variation in epiRILs, RILs, and natural ecotypes of Arabidopsis thaliana. Heredity 121, 257–265 (2018) doi:10.1038/s41437-018-0095-9</small></p>
<p><small> Zheng X et al (2017) Histone acetylation is involved in GA-mediated 45S rDNA decondensation in maize aleurone layers. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2207-z</small></p>
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<h3>Successful ChIP-seq experiments for a variety of plants</h3>
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<div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center>
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</div>
<table style="width: 856px;">
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<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
</td>
<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
</td>
<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
</td>
</tr>
<tr>
<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
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<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
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<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
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<tr>
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<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
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<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
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<h3>Successful ChIP-seq experiments for a variety of plants</h3>
<div class="row">
<div class="small-6 columns">
<h4 class="text-center">Arabidopsis</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG1"> <img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A-small.jpg" /> </a></p>
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<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
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<div class="small-6 columns">
<h4 class="text-center">Poplar</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG2"><img src="https://www.diagenode.com/img/landing-pages/poplar-small.jpg" /> </a></p>
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<a class="close-reveal-modal" aria-label="Close">×</a></div>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
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<div class="row">
<div class="small-6 columns">
<h4 class="text-center">Tomato</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG3"> <img src="https://www.diagenode.com/img/landing-pages/tomtato-small.jpg" /> </a></p>
<div id="IMG3" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">×</a></div>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns">
<h4 class="text-center">Maize</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG4"> <img src="https://www.diagenode.com/img/landing-pages/maize-small.jpg" /> </a></p>
<div id="IMG4" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">×</a></div>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our <a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">Premium H3K27me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
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<p><strong> </strong></p>
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<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
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<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
</tbody>
</table>
<p><strong></strong></p>
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'slug' => 'universal-plant-chip-seq-kit-x24-24-rxns',
'meta_title' => 'Universal Plant ChIP-seq kit | Diagenode',
'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar',
'meta_description' => 'Optimized extraction of plant chromatin from Arabidopsis,maize,rice,tomato,poplar.Complete ChIP kit including plant-specific control primer pairs and antibody',
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'description' => '<p style="text-align: justify;">The <strong>Auto Universal Plant ChIP-seq</strong> kit offers the convenience of extracting plant chromatin from a wide variety of plants including Arabidopsis, maize, rice, tomato and poplar and has been validated for the <strong>IP-Star® automated system</strong>. This complete kit has been specifically optimized for <strong>plant chromatin extraction</strong> and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.</p>',
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<ul>
<li><strong>Universal compatiblity</strong> with a wide variety of plant species</li>
<li>Optimized and <strong>complete kit</strong> for start-to-finish plant ChIP</li>
<li>Validated for the high throughput <strong>IP-Star® Automated System</strong></li>
</ul>
<h3>Successful ChIP-seq experiments for a variety of plants</h3>
<div class="row">
<div class="small-6 columns"><center>Arabidopsis</center><center><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></center>
<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
</div>
<div class="small-6 columns"><center>Poplar</center><center><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></center>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<table style="width: 856px;">
<tbody>
<tr>
<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
</td>
<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
</td>
<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
</td>
</tr>
<tr>
<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
</tbody>
</table>',
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'slug' => 'auto-universal-plant-chip-seq-kit-x24-24-rxns',
'meta_title' => 'Auto Universal Plant ChIP-seq kit | Diagenode',
'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar, automated system, automation, IP-Star',
'meta_description' => 'Plant chromatin extraction from Arabidopsis,maize,rice,tomato,poplar.Complete ChIP kit including plant-specific primer pairs,antibody.Compatible with Automation',
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<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
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<div class="small-12 medium-12 large-12 columns">
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<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
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<p>Check all <a href="https://www.diagenode.com/en/categories/chromatin-shearing">Chromatin EasyShear Kits</a>.</p>
<p>Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – <a href="https://www.diagenode.com/en/pages/chromatin-prep-easyshear-kit-guide">Read more</a></p>
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<p><img src="https://www.diagenode.com/img/areas/plant.jpg" /></p>
</div>
<div class="extra-spaced">
<h2>Epigenetic Regulation in Plants</h2>
<p>Plants utilize a number of gene regulation mechanisms to ensure proper development, function, growth, and survival under different environmental conditions. Plants depend on changes in gene expression to respond to environmental stimuli, in which the full repertoire of histone modifications, DNA methylation, and small ncRNAs play an important role in epigenetic regulation.</p>
<p>Studying the epigenetics of model plants such as Arabidopsis thaliana have allowed researchers to understand pathways that maintain chromatin modifications as well as the mapping of modifications such as DNA methylation on a genome-wide scale. Small RNAs have also been implicated in playing a role in the distribution of chromatin modifications, and RNA may also play a role in the complex epigenetic interactions that occur between homologous sequences (Moazed et al, 2009). In the future, by understanding epigenetic control, researchers can uncover the research necessary to improve plant growth, yields, and transformation efficiency especially in the face of climate change and other environmental factors.</p>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-3 large-3 columns">
<p><img src="https://www.diagenode.com/img/areas/chromatin-and-transcription-factors.jpg" /></p>
</div>
<div class="small-12 medium-9 large-9 columns">
<h3 style="font-weight: 100; margin-top: 0;">Chromatin</h3>
<p>Chromatin consists of nucleosomes formed by a complex of histone proteins and DNA, which allows the packaging of DNA into the nucleus. The less condensed euchromatin represents transcriptionally active regions, while heterochromatin is usually inactive (Vaillant and Paszkowski, 2007). Chromatin state is known to be influenced by both DNA methylation and histone modifications which in turn impact gene expression and the structure of chromosomes. In a recent study, the role of chromatin modifications during plant reproduction elucidated 3-dimensional chromosome reorganization mediated by histones and DNA methylation (Dukowic-Schulze et al. 2017). In addition, gibberellins have been shown in increasing the level of histone acetylation, which affects regions of chromatin involved in maize seed germination (Zheng et al. 2017). Another study reports a novel function of a tomato histone deacetylase gene in the regulation of fruit ripening (Guo et al. 2017).</p>
</div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-3 large-3 columns">
<p><img src="https://www.diagenode.com/img/areas/cherry-tomato-common-grape-vine-ripening-fruit-vegetable-cherry-tomatoes.jpg" /></p>
</div>
<div class="small-12 medium-9 large-9 columns">
<p>In addition, multigene families encode transcription factors, with members found throughout the genome or clustered on the same chromosome. Numerous DNA binding proteins that interact with plant promoters have been identified -- some are similar to well-characterized transcription factors in animals or yeast, while others are unique to plants. For example, diverse members of the subfamily X of the plant-specific ethylene response factor (ERF) transcription factors coordinate stress signaling with wound repair activation. Tissue repair is also enhanced through a protein complex of ERF and GRAS TFs (Heyman et. al,.2018). A compilation of known plant transcription factors can be found in the plant transcription factor database at http://plntfdb.bio.uni-potsdam.de/v3.0/.</p>
</div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-3 large-3 columns">
<p><img src="https://www.diagenode.com/img/areas/rna-strand.jpg" /></p>
</div>
<div class="small-12 medium-9 large-9 columns">
<h3 style="font-weight: 100; margin-top: 0;">RNA</h3>
<p>Recent research shows that a number of classes of small RNAs are key epigenetic regulators. In many cases, small RNAs have been implicated in DNA methylation and chromatin modification (Meyer, 2015). In addition, the role of small RNAs has been implicated in plant stress tolerance (Kumar et al., 2017). López-Galiano et al also provided insight into a coordinated function of a miRNA gene and histone modifications in regulating the expression of a WRKY transcription factor in response to stress.</p>
<p>RNA interference (RNAi) is another epigenetic mechanism that leads to small RNA generation, which mediates gene silencing at the post-transcriptional level. RNAi technology has immense potential for plant disease resistance.</p>
</div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-3 large-3 columns">
<p><img src="https://www.diagenode.com/img/areas/dna-methylation.jpg" /></p>
</div>
<div class="small-12 medium-9 large-9 columns">
<h3 style="font-weight: 100; margin-top: 0;">DNA methylation</h3>
<p>Plants, unlike animals, have three sites that can be methylated G, CHG (H can be A, C, T), and CHH (Law and Jacobsen, 2010). DNA methylation has attracted particular interest. In Arabidopsis, one-third of methylated genes occur in transcribed regions, and 5% of genes are methylated in promoter regions, suggesting that many of these are epigenetically regulated. (Zhang et al., 2006).</p>
<p>There are thousands of differentially methylated regions (DMRs) that influence phenotype by influencing gene expression. The analysis of epigenetic recombinant inbred line (epiRIL) plants from Arabidopsis points to the evidence of the influence of DMRs. An epiRIL results from crossing two genetically identical plants with differing DNA methylation levels (with one parent as a homozygous mutant for an essential DNA methylation maintenance gene). The offspring of these plants have similar genomes that vary only in methylation levels. Many traits have been studied using epiRILs -- flowering time, plant height, and response to abiotic stress, some of which have now been mapped to DMRs (Zhang et al. 2018)</p>
<p>Regulation by DNA methylation has been shown to be important in many aspects of plant development and response such as vernalization, hybrid vigor, and self-incompatibility (Itabashi et al. 2017). For example, vernalization treatments have shown reduced DNA methylation and subsequent initiation of flowering (Burn et al., 1993). Stress can also influence DNA methylation in plants as a response to environmental stimuli. (Steward et al., 2002; Song et al., 2012). A high degree of DNA methylation has also suggested the role in the improvement of plant fitness under different environmental conditions (Saéz-Laguna et al., 2014). In addition, methylation can affect normal fruit and hypomethylation predicts homeotic transformation and loss of fruit yield (Ong-Abdullah et al., 2015)</p>
</div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-3 large-3 columns">
<p><img src="https://www.diagenode.com/img/areas/plant-development.jpg" class="left" style="padding-right: 15px;" /></p>
</div>
<div class="small-12 medium-9 large-9 columns">
<p>DNA demethylation has also been implied in various aspects of plant development including pollen tube formation, embryogenesis, fruit ripening, stomatal development, and nodule formation ( Li et al. 2017). Demethylation of rice genomic DNA caused an altered pattern of gene expression, inducing dwarf plants (Sano et al., 1990).</p>
<p>Epigenetic modifications contribute to the stability and survival of the plants and their ability to adapt in different environmental conditions.</p>
</div>
</div>
<h3>Diagenode products for your epigenomics research in plants</h3>
<div class="row extra-spaced">
<div class="small-12 medium-4 large-4 columns text-left">
<div class="panel" style="border-color: #099f92; height: 275px;">
<h3 class="text-center"><a href="https://www.diagenode.com/en/categories/chromatin-function">Chromatin analysis</a></h3>
<center><a href="https://www.diagenode.com/en/categories/chromatin-function"><img src="https://www.diagenode.com/img/cancer/chromatin-icon.png" /></a></center>
<p class="text-left">Understand the role of chromatin in plant function and development</p>
</div>
<ul>
<li><a href="https://www.diagenode.com/en/categories/chromatin-function">Learn about our chromatin analysis products</a></li>
<li><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns"> Learn about the Universal Plant ChIP Kit</a></li>
</ul>
</div>
<div class="small-12 medium-4 large-4 columns text-left">
<div class="panel" style="border-color: #30415c; height: 275px;">
<h3 class="text-center"><a href="https://www.diagenode.com/en/categories/dna-methylation" style="color: #30415c;">DNA methylation</a></h3>
<center><a href="https://www.diagenode.com/en/categories/dna-methylation"><img src="https://www.diagenode.com/img/cancer/dna-icon.png" /></a></center>
<p class="text-left">DNA methylation and demethylation and the effects on plant response and function</p>
</div>
<ul>
<li><a href="https://www.diagenode.com/en/categories/dna-methylation">Discover DNA methylation analysis solutions at any resolution</a></li>
</ul>
</div>
<div class="small-12 medium-4 large-4 columns text-left">
<div class="panel" style="border-color: #474546; height: 275px;">
<h3 class="text-center"><span class="darkgrey">Non-coding RNAs</span></h3>
<center><img src="https://www.diagenode.com/img/cancer/non-coding-icon.png" /></center>
<p class="text-left">Discover noncoding RNAs in the regulation of gene expression in plants</p>
</div>
<ul>
<li><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">Library prep for RNA-seq studies for ncRNAs</a></li>
</ul>
</div>
</div>
<h3>References</h3>
<p><small> Burn, J. et al (1993). DNA methylation, vernalization, and the initiation of flowering. Proc. Natl. Acad. Sci. U.S.A. 90, 287–291. doi: 10.1006/scdb.1996.0055 </small></p>
<p><small> Dukowic-Schulze S, Liu C, Chen C (2017) Not just gene expression: 3D implications of chromatin modifications during sexual plant reproduction. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2222-0</small></p>
<p><small> Guo J et al (2017) A histone deacetylase gene, SlHDA3, acts as a negative regulator of fruit ripening and carotenoid accumulation. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2211-3</small></p>
<p><small> Heyman J, et.al (2018) Journal of Cell Science Emerging role of the plant ERF transcription factors in coordinating wound defense responses and repair doi: 10.1242/jcs.208215</small></p>
<p><small> Itabashi E, Osabe K, Fujimoto R, Kakizaki T (2017) Epigenetic regulation of agronomical traits in Brassicaceae. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2223-z</small></p>
<p><small> Kumar V et al (2017) Plant small RNAs: the essential epigenetic regulators of gene expression for salt-stress responses and tolerance. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2210-4</small></p>
<p><small> Law, J. A., and Jacobsen, S. E. (2010). Establishing, maintaining and modifying DNA methylation patterns in plants and animals. Nat. Rev. Genet. 11, 204–220. doi: 10.1038/nrg2719</small></p>
<p><small> Meyer, P. (2015). Epigenetic variation and environmental change. J. Exp. Bot. 66, 3541–3548. doi: 10.1093/jxb/eru502</small></p>
<p><small> Moazed, D. (2009) Small RNAs in transcriptional gene silencing and genome defence. Nature. doi: 10.1038/nature07756</small></p>
<p><small> Ong-Abdullah et al. (2015). Loss of Karma transposon methylation underlies the mantled somaclonal variant of oil palm. Nature 525, 533–537. doi: 10.1038/nature15365</small></p>
<p><small> Saéz-Laguna et al. (2014). Epigenetic variability in the genetically uniform forest tree species. PLoS One 9:e103145. doi: 10.1371/journal.pone.0103145</small></p>
<p><small> Sano, H. et al. (1990). A single treatment of rice seedlings with 5-azacytidine induces heritable dwarfism and undermethylation of genomic DNA. Mol. Gen. Genet. 220, 441–447. doi: 10.1007/BF00391751</small></p>
<p><small> Song, J et al (2012). Vernalization – A cold-induced epigenetic switch. J. Cell Sci. 125, 3723–3731. doi: 10.1242/jcs.084764</small></p>
<p><small> Steward, N et al. (2002). Periodic DNA methylation in maize nucleosomes and demethylation by environmental stress. J. Biol. Chem. 277, 37741–37746. doi: 10.1074/jbc.M204050200</small></p>
<p><small> Vaillant, I., and Paszkowski, J. (2007). Role of histone and DNA methylation in gene regulation. Curr. Opin. Plant Biol. 10, 528–533. doi: 10.1016/j.pbi.2007.06.008</small></p>
<p><small> Zhang, et al. (2006). Genome-wide high-resolution mapping and functional analysis of DNA methylation in Arabidopsis. Cell 126, 1189–1201. doi: 10.1016/j.cell.2006.08.003</small></p>
<p><small> Zhang et al. 2018 Understanding the evolutionary potential of epigenetic variation: a comparison of heritable phenotypic variation in epiRILs, RILs, and natural ecotypes of Arabidopsis thaliana. Heredity 121, 257–265 (2018) doi:10.1038/s41437-018-0095-9</small></p>
<p><small> Zheng X et al (2017) Histone acetylation is involved in GA-mediated 45S rDNA decondensation in maize aleurone layers. Plant Cell Rep. https://dx.doi.org/10.1007/s00299-017-2207-z</small></p>
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<h3>Successful ChIP-seq experiments for a variety of plants</h3>
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<div class="small-6 columns"><center>Arabidopsis</center><center><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></center>
<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
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<div class="small-6 columns"><center>Poplar</center><center><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></center>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
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<div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
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<div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
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<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
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<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
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<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
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<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
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<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
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<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
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)
)
$sds = array(
'id' => '900',
'name' => 'Chromatin EasyShear Kit for Plant SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Chromatin_EasyShear_Kit-Plant/SDS-C01020014-Chromatin_EasyShear_Kit_for_Plant-ES-es-1_0.pdf',
'countries' => 'ES',
'modified' => '2020-09-22 15:26:00',
'created' => '2020-09-22 15:26:00',
'ProductsSafetySheet' => array(
'id' => '1603',
'product_id' => '2789',
'safety_sheet_id' => '900'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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