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<p><small><strong>Figure 1. Determination of the titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse ctr9/PAF1 (Cat. No. CS-112-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:4,600. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ctr9/PAF1 (1)</strong><br /> Western blot was performed on nuclear extracts and whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse ctr9/PAF1 (Cat. No. CS-112-100), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 133 kDa) is indicated on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><small><strong>Figure 1. Determination of the titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse ctr9/PAF1 (Cat. No. CS-112-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:4,600. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ctr9/PAF1 (1)</strong><br /> Western blot was performed on nuclear extracts and whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse ctr9/PAF1 (Cat. No. CS-112-100), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 133 kDa) is indicated on the right.</small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ctr9/PAF1 (1)</strong><br /> Western blot was performed on nuclear extracts and whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse ctr9/PAF1 (Cat. No. CS-112-100), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 133 kDa) is indicated on the right.</small></p>
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$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
'id' => '1635',
'product_id' => '2102',
'document_id' => '38'
)
)
$sds = array(
'id' => '2836',
'name' => 'ctr9/PAF1 Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/ctr9_PAF1/SDS-C15310112-ctr9_PAF1_Antibody-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2022-10-07 12:27:42',
'created' => '2022-10-07 12:27:42',
'ProductsSafetySheet' => array(
'id' => '4676',
'product_id' => '2102',
'safety_sheet_id' => '2836'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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