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Figure 1. IP ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell extract A. 40 μg 293T whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody. The immunoprecipitated ERR alpha protein was detected by western blot with the ERR alpha antibody (Cat. No. C15410230) diluted 1:1,000.
Figure 2. Western blot Sample: 30 μg of whole cell lysate A: 293T B: A431 C: H1299 12% SDS PAGE C15410230 diluted 1:500
Figure 3. ERR alpha antibody ChIP results ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or ERR alpha antibody. The precipitated DNA was detected by PCR with primer set, targeting to PS2 promoter, used as a positive control or GAPDH promoter, used as a negative control.
Figure 4. ERR alpha antibody WB results ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg mouse kidney extract 10 % SDS-PAGE ERR alpha antibody (cat. No. C15410230) dilution 1:500
Figure 5. ERR alpha antibody WB results ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg rat kidney extract 10 % SDS-PAGE ERR alpha antibody (Cat. No. C15410230) dilution 1:500
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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