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<p><small><strong> Figure 1. IP</strong><br /> ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell extract A. 40 μg 293T whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody. The immunoprecipitated ERR alpha protein was detected by western blot with the ERR alpha antibody (Cat. No. C15410230) diluted 1:1,000. </small></p>
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<p><small><strong> Figure 2. Western blot</strong><br /> Sample: 30 μg of whole cell lysate A: 293T B: A431 C: H1299 12% SDS PAGE C15410230 diluted 1:500 </small></p>
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<p><small><strong> Figure 3. ERR alpha antibody ChIP results</strong><br /> ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or ERR alpha antibody. The precipitated DNA was detected by PCR with primer set, targeting to PS2 promoter, used as a positive control or GAPDH promoter, used as a negative control. </small></p>
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<p><small><strong> Figure 4. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg mouse kidney extract 10 % SDS-PAGE ERR alpha antibody (cat. No. C15410230) dilution 1:500 </small></p>
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<p><small><strong> Figure 5. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg rat kidney extract 10 % SDS-PAGE ERR alpha antibody (Cat. No. C15410230) dilution 1:500 </small></p>
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<p><small><strong> Figure 1. IP</strong><br /> ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell extract A. 40 μg 293T whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody. The immunoprecipitated ERR alpha protein was detected by western blot with the ERR alpha antibody (Cat. No. C15410230) diluted 1:1,000. </small></p>
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<p><small><strong> Figure 2. Western blot</strong><br /> Sample: 30 μg of whole cell lysate A: 293T B: A431 C: H1299 12% SDS PAGE C15410230 diluted 1:500 </small></p>
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<p><small><strong> Figure 3. ERR alpha antibody ChIP results</strong><br /> ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or ERR alpha antibody. The precipitated DNA was detected by PCR with primer set, targeting to PS2 promoter, used as a positive control or GAPDH promoter, used as a negative control. </small></p>
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<p><small><strong> Figure 4. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg mouse kidney extract 10 % SDS-PAGE ERR alpha antibody (cat. No. C15410230) dilution 1:500 </small></p>
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<p><small><strong> Figure 5. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg rat kidney extract 10 % SDS-PAGE ERR alpha antibody (Cat. No. C15410230) dilution 1:500 </small></p>
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<p><small><strong> Figure 1. IP</strong><br /> ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell extract A. 40 μg 293T whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody. The immunoprecipitated ERR alpha protein was detected by western blot with the ERR alpha antibody (Cat. No. C15410230) diluted 1:1,000. </small></p>
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<p><small><strong> Figure 3. ERR alpha antibody ChIP results</strong><br /> ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or ERR alpha antibody. The precipitated DNA was detected by PCR with primer set, targeting to PS2 promoter, used as a positive control or GAPDH promoter, used as a negative control. </small></p>
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<p><small><strong> Figure 4. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg mouse kidney extract 10 % SDS-PAGE ERR alpha antibody (cat. No. C15410230) dilution 1:500 </small></p>
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<p><small><strong> Figure 5. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg rat kidney extract 10 % SDS-PAGE ERR alpha antibody (Cat. No. C15410230) dilution 1:500 </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410230-ERR-IP.jpg" alt="ERR alpha Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 1. IP</strong><br /> ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell extract A. 40 μg 293T whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody. The immunoprecipitated ERR alpha protein was detected by western blot with the ERR alpha antibody (Cat. No. C15410230) diluted 1:1,000. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410230-ERR-WB.jpg" alt="ERR alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2. Western blot</strong><br /> Sample: 30 μg of whole cell lysate A: 293T B: A431 C: H1299 12% SDS PAGE C15410230 diluted 1:500 </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410230-ERR-ChIP.jpg" alt="ERR alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. ERR alpha antibody ChIP results</strong><br /> ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or ERR alpha antibody. The precipitated DNA was detected by PCR with primer set, targeting to PS2 promoter, used as a positive control or GAPDH promoter, used as a negative control. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410230-ERR-WB-2.jpg" alt="ERR alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg mouse kidney extract 10 % SDS-PAGE ERR alpha antibody (cat. No. C15410230) dilution 1:500 </small></p>
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<div class="row">
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410230-ERR-WB-3.jpg" alt="ERR alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 5. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg rat kidney extract 10 % SDS-PAGE ERR alpha antibody (Cat. No. C15410230) dilution 1:500 </small></p>
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<p><small><strong> Figure 1. IP</strong><br /> ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell extract A. 40 μg 293T whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody. The immunoprecipitated ERR alpha protein was detected by western blot with the ERR alpha antibody (Cat. No. C15410230) diluted 1:1,000. </small></p>
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<p><small><strong> Figure 2. Western blot</strong><br /> Sample: 30 μg of whole cell lysate A: 293T B: A431 C: H1299 12% SDS PAGE C15410230 diluted 1:500 </small></p>
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<p><small><strong> Figure 3. ERR alpha antibody ChIP results</strong><br /> ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or ERR alpha antibody. The precipitated DNA was detected by PCR with primer set, targeting to PS2 promoter, used as a positive control or GAPDH promoter, used as a negative control. </small></p>
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<p><small><strong> Figure 4. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg mouse kidney extract 10 % SDS-PAGE ERR alpha antibody (cat. No. C15410230) dilution 1:500 </small></p>
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<p><small><strong> Figure 5. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg rat kidney extract 10 % SDS-PAGE ERR alpha antibody (Cat. No. C15410230) dilution 1:500 </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong> Figure 1. IP</strong><br /> ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell extract A. 40 μg 293T whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody. The immunoprecipitated ERR alpha protein was detected by western blot with the ERR alpha antibody (Cat. No. C15410230) diluted 1:1,000. </small></p>
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<p><small><strong> Figure 2. Western blot</strong><br /> Sample: 30 μg of whole cell lysate A: 293T B: A431 C: H1299 12% SDS PAGE C15410230 diluted 1:500 </small></p>
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<p><small><strong> Figure 4. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg mouse kidney extract 10 % SDS-PAGE ERR alpha antibody (cat. No. C15410230) dilution 1:500 </small></p>
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<p><small><strong> Figure 5. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg rat kidney extract 10 % SDS-PAGE ERR alpha antibody (Cat. No. C15410230) dilution 1:500 </small></p>
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<p><small><strong> Figure 1. IP</strong><br /> ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell extract A. 40 μg 293T whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody. The immunoprecipitated ERR alpha protein was detected by western blot with the ERR alpha antibody (Cat. No. C15410230) diluted 1:1,000. </small></p>
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<p><small><strong> Figure 3. ERR alpha antibody ChIP results</strong><br /> ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or ERR alpha antibody. The precipitated DNA was detected by PCR with primer set, targeting to PS2 promoter, used as a positive control or GAPDH promoter, used as a negative control. </small></p>
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<p><small><strong> Figure 4. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg mouse kidney extract 10 % SDS-PAGE ERR alpha antibody (cat. No. C15410230) dilution 1:500 </small></p>
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<p><small><strong> Figure 5. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg rat kidney extract 10 % SDS-PAGE ERR alpha antibody (Cat. No. C15410230) dilution 1:500 </small></p>
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<p><small><strong> Figure 2. Western blot</strong><br /> Sample: 30 μg of whole cell lysate A: 293T B: A431 C: H1299 12% SDS PAGE C15410230 diluted 1:500 </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410230-ERR-ChIP.jpg" alt="ERR alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. ERR alpha antibody ChIP results</strong><br /> ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or ERR alpha antibody. The precipitated DNA was detected by PCR with primer set, targeting to PS2 promoter, used as a positive control or GAPDH promoter, used as a negative control. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410230-ERR-WB-2.jpg" alt="ERR alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg mouse kidney extract 10 % SDS-PAGE ERR alpha antibody (cat. No. C15410230) dilution 1:500 </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410230-ERR-WB-3.jpg" alt="ERR alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg rat kidney extract 10 % SDS-PAGE ERR alpha antibody (Cat. No. C15410230) dilution 1:500 </small></p>
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<p><small><strong> Figure 1. IP</strong><br /> ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell extract A. 40 μg 293T whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody. The immunoprecipitated ERR alpha protein was detected by western blot with the ERR alpha antibody (Cat. No. C15410230) diluted 1:1,000. </small></p>
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<p><small><strong> Figure 2. Western blot</strong><br /> Sample: 30 μg of whole cell lysate A: 293T B: A431 C: H1299 12% SDS PAGE C15410230 diluted 1:500 </small></p>
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<p><small><strong> Figure 3. ERR alpha antibody ChIP results</strong><br /> ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or ERR alpha antibody. The precipitated DNA was detected by PCR with primer set, targeting to PS2 promoter, used as a positive control or GAPDH promoter, used as a negative control. </small></p>
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<p><small><strong> Figure 4. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg mouse kidney extract 10 % SDS-PAGE ERR alpha antibody (cat. No. C15410230) dilution 1:500 </small></p>
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<p><small><strong> Figure 5. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg rat kidney extract 10 % SDS-PAGE ERR alpha antibody (Cat. No. C15410230) dilution 1:500 </small></p>
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<p><small><strong> Figure 3. ERR alpha antibody ChIP results</strong><br /> ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or ERR alpha antibody. The precipitated DNA was detected by PCR with primer set, targeting to PS2 promoter, used as a positive control or GAPDH promoter, used as a negative control. </small></p>
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<p><small><strong> Figure 4. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg mouse kidney extract 10 % SDS-PAGE ERR alpha antibody (cat. No. C15410230) dilution 1:500 </small></p>
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<p><small><strong> Figure 5. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg rat kidney extract 10 % SDS-PAGE ERR alpha antibody (Cat. No. C15410230) dilution 1:500 </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410230-ERR-IP.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 1. IP</strong><br /> ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell extract A. 40 μg 293T whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody. The immunoprecipitated ERR alpha protein was detected by western blot with the ERR alpha antibody (Cat. No. C15410230) diluted 1:1,000. </small></p>
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<p><small><strong> Figure 2. Western blot</strong><br /> Sample: 30 μg of whole cell lysate A: 293T B: A431 C: H1299 12% SDS PAGE C15410230 diluted 1:500 </small></p>
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<p><small><strong> Figure 3. ERR alpha antibody ChIP results</strong><br /> ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or ERR alpha antibody. The precipitated DNA was detected by PCR with primer set, targeting to PS2 promoter, used as a positive control or GAPDH promoter, used as a negative control. </small></p>
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<p><small><strong> Figure 4. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg mouse kidney extract 10 % SDS-PAGE ERR alpha antibody (cat. No. C15410230) dilution 1:500 </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410230-ERR-WB-2.jpg" alt="Western blot figure 3" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 5. ERR alpha antibody WB results</strong><br /> ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg rat kidney extract 10 % SDS-PAGE ERR alpha antibody (Cat. No. C15410230) dilution 1:500 </small></p>
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