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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p>
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<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p>
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<td>1:100 - 1:1,000</td>
<td>Fig 6</td>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p>
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<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p>
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<p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p>
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<td>Fig 1, 2</td>
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<td>1:100 - 1:1,000</td>
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<td>1:100 - 1:1,000</td>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p>Polyclonal antibody raised in rabbit against FOXA1 (forkhead box A1), using a KLH-conjugated synthetic peptide.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="ChIP-seq figure 3" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="ChIP-seq figure 4" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p>
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<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<div class="small-8 columns">
<p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p>
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<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p>
</div>
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'meta_description' => 'FOXA1 (Forkhead box A1) Polyclonal Antibody validated in WB, IF, IP, IHC, ChIP-seq and ChIP-qPCR. Alternative names: HNF3A, HNF-3A, TCF3A, TCF-3A.',
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'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution <sup>*</sup></th>
<th>References</th>
</tr>
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<tbody>
<tr>
<td>ChIP/ChIP-seq</td>
<td>2 μg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:500 - 1:3,000</td>
<td>Fig 3</td>
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<tr>
<td>Immunoprecipitation</td>
<td>2.5 μg/IP</td>
<td>Fig 4</td>
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<tr>
<td>Immunofluorescence</td>
<td>1:100 - 1:1,000</td>
<td>Fig 5</td>
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<tr>
<td>Immunohistochemistry</td>
<td>1:100 - 1:1,000</td>
<td>Fig 6</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>',
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'description' => '<p>Alternative names: <strong>HNF3A</strong>, <strong>HNF-3A</strong>, <strong>TCF3A</strong>, <strong>TCF-3A</strong></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIP.jpg" alt="FOXA1 Antibody ChIP Grade" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p>
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> Expert technical support</span></li>
<li><span style="font-weight: 400;"> Sample sizes available</span></li>
<li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p>
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<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p>
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<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p>
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<p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p>
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<td>Fig 6</td>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p>
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<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<div class="small-4 columns">
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<p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
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<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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'id' => '2315',
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'name' => 'FOXA1 polyclonal antibody - Classic',
'description' => '<p>Alternative names: <strong>HNF3A</strong>, <strong>HNF-3A</strong>, <strong>TCF3A</strong>, <strong>TCF-3A</strong></p>
<p>Polyclonal antibody raised in rabbit against FOXA1 (forkhead box A1), using a KLH-conjugated synthetic peptide.</p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIP.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="ChIP-seq figure 3" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="ChIP-seq figure 4" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p>
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<p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p>
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<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p>
</div>
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<thead>
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<th>Applications</th>
<th>Suggested dilution <sup>*</sup></th>
<th>References</th>
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<tr>
<td>ChIP/ChIP-seq</td>
<td>2 μg/ChIP</td>
<td>Fig 1, 2</td>
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<tr>
<td>Western Blotting</td>
<td>1:500 - 1:3,000</td>
<td>Fig 3</td>
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<td>Immunoprecipitation</td>
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<td>Fig 4</td>
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<td>Immunofluorescence</td>
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<td>1:100 - 1:1,000</td>
<td>Fig 6</td>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p>Polyclonal antibody raised in rabbit against FOXA1 (forkhead box A1), using a KLH-conjugated synthetic peptide.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="ChIP-seq figure 3" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p>
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<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p>
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