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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXM1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against FOXM1 (Cat. No. C15410232) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the CCNA2 and CCNB1 genes, used as positive controls, and for the IFT80 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXM1</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against FOXM1 (Cat. No. C15410232) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 kb region of human chromosome 6 (fig 2A and B), and in two genomic regions surrounding the CCNA2 and CCNB1 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXM1</strong><br /> Whole cell extracts from 293T (30 μg, lane 1) or 293T cells transfected with a FOXM1 expression vector (lane 2) were analysed by Western blot using the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:5,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunohistochemistry using the Diagenode antibody directed against FOXM1</strong><br /> Formalin fixed paraffin embedded human ovarian cancer tissue was stained with the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:100 followed by a peroxidase labelled goat anti-rabbit secondary antibody.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXM1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against FOXM1 (Cat. No. C15410232) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the CCNA2 and CCNB1 genes, used as positive controls, and for the IFT80 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXM1</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against FOXM1 (Cat. No. C15410232) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 kb region of human chromosome 6 (fig 2A and B), and in two genomic regions surrounding the CCNA2 and CCNB1 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXM1</strong><br /> Whole cell extracts from 293T (30 μg, lane 1) or 293T cells transfected with a FOXM1 expression vector (lane 2) were analysed by Western blot using the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:5,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunohistochemistry using the Diagenode antibody directed against FOXM1</strong><br /> Formalin fixed paraffin embedded human ovarian cancer tissue was stained with the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:100 followed by a peroxidase labelled goat anti-rabbit secondary antibody.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXM1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against FOXM1 (Cat. No. C15410232) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the CCNA2 and CCNB1 genes, used as positive controls, and for the IFT80 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXM1</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against FOXM1 (Cat. No. C15410232) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 kb region of human chromosome 6 (fig 2A and B), and in two genomic regions surrounding the CCNA2 and CCNB1 positive control genes (fig 2C and D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410232_FOXM1_lot 42613_fig3_WB.jpg" alt="FOXM1 Antibody validated in Western Blot" /></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXM1</strong><br /> Whole cell extracts from 293T (30 μg, lane 1) or 293T cells transfected with a FOXM1 expression vector (lane 2) were analysed by Western blot using the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:5,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunohistochemistry using the Diagenode antibody directed against FOXM1</strong><br /> Formalin fixed paraffin embedded human ovarian cancer tissue was stained with the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:100 followed by a peroxidase labelled goat anti-rabbit secondary antibody.</small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXM1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against FOXM1 (Cat. No. C15410232) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the CCNA2 and CCNB1 genes, used as positive controls, and for the IFT80 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXM1</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against FOXM1 (Cat. No. C15410232) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 kb region of human chromosome 6 (fig 2A and B), and in two genomic regions surrounding the CCNA2 and CCNB1 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXM1</strong><br /> Whole cell extracts from 293T (30 μg, lane 1) or 293T cells transfected with a FOXM1 expression vector (lane 2) were analysed by Western blot using the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:5,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunohistochemistry using the Diagenode antibody directed against FOXM1</strong><br /> Formalin fixed paraffin embedded human ovarian cancer tissue was stained with the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:100 followed by a peroxidase labelled goat anti-rabbit secondary antibody.</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXM1</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against FOXM1 (Cat. No. C15410232) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 kb region of human chromosome 6 (fig 2A and B), and in two genomic regions surrounding the CCNA2 and CCNB1 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXM1</strong><br /> Whole cell extracts from 293T (30 μg, lane 1) or 293T cells transfected with a FOXM1 expression vector (lane 2) were analysed by Western blot using the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:5,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunohistochemistry using the Diagenode antibody directed against FOXM1</strong><br /> Formalin fixed paraffin embedded human ovarian cancer tissue was stained with the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:100 followed by a peroxidase labelled goat anti-rabbit secondary antibody.</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXM1</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against FOXM1 (Cat. No. C15410232) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 kb region of human chromosome 6 (fig 2A and B), and in two genomic regions surrounding the CCNA2 and CCNB1 positive control genes (fig 2C and D).</small></p>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXM1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against FOXM1 (Cat. No. C15410232) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the CCNA2 and CCNB1 genes, used as positive controls, and for the IFT80 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXM1</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against FOXM1 (Cat. No. C15410232) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 kb region of human chromosome 6 (fig 2A and B), and in two genomic regions surrounding the CCNA2 and CCNB1 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXM1</strong><br /> Whole cell extracts from 293T (30 μg, lane 1) or 293T cells transfected with a FOXM1 expression vector (lane 2) were analysed by Western blot using the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:5,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunohistochemistry using the Diagenode antibody directed against FOXM1</strong><br /> Formalin fixed paraffin embedded human ovarian cancer tissue was stained with the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:100 followed by a peroxidase labelled goat anti-rabbit secondary antibody.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXM1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against FOXM1 (Cat. No. C15410232) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the CCNA2 and CCNB1 genes, used as positive controls, and for the IFT80 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXM1</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against FOXM1 (Cat. No. C15410232) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 kb region of human chromosome 6 (fig 2A and B), and in two genomic regions surrounding the CCNA2 and CCNB1 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXM1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against FOXM1 (Cat. No. C15410232) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the CCNA2 and CCNB1 genes, used as positive controls, and for the IFT80 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXM1</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against FOXM1 (Cat. No. C15410232) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 kb region of human chromosome 6 (fig 2A and B), and in two genomic regions surrounding the CCNA2 and CCNB1 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXM1</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against FOXM1 (Cat. No. C15410232) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 kb region of human chromosome 6 (fig 2A and B), and in two genomic regions surrounding the CCNA2 and CCNB1 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXM1</strong><br /> Whole cell extracts from 293T (30 μg, lane 1) or 293T cells transfected with a FOXM1 expression vector (lane 2) were analysed by Western blot using the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:5,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXM1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against FOXM1 (Cat. No. C15410232) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the CCNA2 and CCNB1 genes, used as positive controls, and for the IFT80 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXM1</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against FOXM1 (Cat. No. C15410232) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 kb region of human chromosome 6 (fig 2A and B), and in two genomic regions surrounding the CCNA2 and CCNB1 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 4. Immunohistochemistry using the Diagenode antibody directed against FOXM1</strong><br /> Formalin fixed paraffin embedded human ovarian cancer tissue was stained with the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:100 followed by a peroxidase labelled goat anti-rabbit secondary antibody.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXM1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against FOXM1 (Cat. No. C15410232) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the CCNA2 and CCNB1 genes, used as positive controls, and for the IFT80 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXM1</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against FOXM1 (Cat. No. C15410232) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 kb region of human chromosome 6 (fig 2A and B), and in two genomic regions surrounding the CCNA2 and CCNB1 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXM1</strong><br /> Whole cell extracts from 293T (30 μg, lane 1) or 293T cells transfected with a FOXM1 expression vector (lane 2) were analysed by Western blot using the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:5,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunohistochemistry using the Diagenode antibody directed against FOXM1</strong><br /> Formalin fixed paraffin embedded human ovarian cancer tissue was stained with the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:100 followed by a peroxidase labelled goat anti-rabbit secondary antibody.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXM1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against FOXM1 (Cat. No. C15410232) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the CCNA2 and CCNB1 genes, used as positive controls, and for the IFT80 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXM1</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against FOXM1 (Cat. No. C15410232) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 kb region of human chromosome 6 (fig 2A and B), and in two genomic regions surrounding the CCNA2 and CCNB1 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 4. Immunohistochemistry using the Diagenode antibody directed against FOXM1</strong><br /> Formalin fixed paraffin embedded human ovarian cancer tissue was stained with the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:100 followed by a peroxidase labelled goat anti-rabbit secondary antibody.</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXM1</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against FOXM1 (Cat. No. C15410232) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 kb region of human chromosome 6 (fig 2A and B), and in two genomic regions surrounding the CCNA2 and CCNB1 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXM1</strong><br /> Whole cell extracts from 293T (30 μg, lane 1) or 293T cells transfected with a FOXM1 expression vector (lane 2) were analysed by Western blot using the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:5,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXM1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against FOXM1 (Cat. No. C15410232) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the CCNA2 and CCNB1 genes, used as positive controls, and for the IFT80 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410232-chipseq-A.jpg" alt="ChIP-seq" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXM1</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against FOXM1 (Cat. No. C15410232) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 kb region of human chromosome 6 (fig 2A and B), and in two genomic regions surrounding the CCNA2 and CCNB1 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXM1</strong><br /> Whole cell extracts from 293T (30 μg, lane 1) or 293T cells transfected with a FOXM1 expression vector (lane 2) were analysed by Western blot using the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:5,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunohistochemistry using the Diagenode antibody directed against FOXM1</strong><br /> Formalin fixed paraffin embedded human ovarian cancer tissue was stained with the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:100 followed by a peroxidase labelled goat anti-rabbit secondary antibody.</small></p>
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