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GR Antibody - ChIP-seq Grade (sample size)

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Antibodies

目录号

格式

价格

C15200010-10

10 µg

$115.00
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其他格式

C15200010

50 µg

Alternative names: NR3C1, GCCR, GCR, GRL

Monoclonal antibody raised in mouse against amino acids 304-428 of the human GR (glucocorticoid receptor), using a chimeric protein.

Lot	NR-010
Concentration	1.0 µg/µl
Species reactivity	Human, rat
Type	Monoclonal
Purity	Ammonium sulphate purified
Host	Mouse
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ChIP/ChIP-seq	5 μg/ChIP	Fig 1, 2
ELISA	0.5 μg/ml	Fig 3
Western Blotting	1 μg/ml	Fig 4
Gel Supershift	5 μg/ml
Immunochemistry	2.5 μg/ml	Fig 5, 6
Flow cytometry	0.5 μg/ml
Immunoprecipitation	5 μg/IP	Fig 7

Validation data

Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against hGR
ChIP assays were performed using HeLa cells, the Diagenode monoclonal antibody directed against GR (Cat. No. MAb-010-050) and optimized PCR primer sets for qPCR. The cells were treated either with ethanol (EtOH, used as a negative control) or triamcinolone acetonide (TA) for 4 hours prior to cell harvesting. ChIP was performed using sheared chromatin from 3 million cells and 5 μg of antibody. QPCR was performed with primers for the human metallothionein promoter (hMTIIA) and for exon 2 of the human myoglobin gene (hmyo ex2), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the human metallothionein IIA promoter by GR.

Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against hGR
ChIP was performed on sheared chromatin from 3.5 million HeLaB2 cells using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). The cells were treated with the synthetic GR ligand triamcinolone acetonide (TA) for 4 hours prior to harvesting. The IP’d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence of chromosome 16 (figure 2A) as well as the MT2A positive control gene (figure 2B). The position of the PCR amplicon is also indicated. Figure 2C, D and E show the results for the known GR target genes PER1 on chromosome 17 and FKBP5 and TNFAIP3 on chromosome 6.

Figure 3. Sandwich ELISA
The specificity of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050) was assessed by sandwich ELISA. Figure 3A: schematic representation of the sandwich ELISA with the monoclonal antibody against hGR (clone #:m2F8). Figure 3B: ELISA results using the monoclonal antibody against hGR at a concentration of 0.5 μg/ml. The figure shows an ELISA signal which is proportionally increasing with increasing amounts of recombinant hGR.

Figure 4. Western blot analysis using the Diagenode monoclonal antibody against hGR
Figure 4A. Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10e6 Raji or Molt cells were analysed by Western blot using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). Figure 4B. Western blot analysis of extracts from 300,000 HeLa cells with the Diagenode monoclonal antibody against hGR (concentration 1 μg/ml).

Figure 5. Immunohistochemistry and immunofluorescence using the Diagenode monoclonal antibody against hGR
1. Immunoreactivity of the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050) in rat CA1 neurons of hippocampus. The antibody was used at a concentration of 2.5 μg/ml.
2. COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary antibody and peroxidase-labeled avidin. The antibody was used at a concentration of 2.5 μg/ml.

Figure 6. Immunoprecipitation using the Diagenode monoclonal antibody against hGR
The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 μl IP reaction solution) using 5 μg of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050). The IP was followed by Western blot analysis as described above
Target Description
Monoclonal antibody raised in mouse against amino acids 304-428 of the human GR (glucocorticoid receptor), using a chimeric protein.

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Coordinated glucocorticoid receptor and MAFB action inducestolerogenesis and epigenome remodeling in dendritic cells
Morante-Palacios Octavio et al.
Abstract Glucocorticoids (GCs) exert potent anti-inflammatory effects in immune cells through the glucocorticoid receptor (GR). Dendritic cells (DCs), central actors for coordinating immune responses, acquire tolerogenic properties in response to GCs. Tolerogenic DCs (tolDCs) have emerged as a potential treatment fo...

The Role of S-Palmitoylation of the Human Glucocorticoid Receptor (hGR) in Mediating the Nongenomic Glucocorticoid Actions
Nicolaides N. et al.
Background: Many rapid nongenomic glucocorticoid actions are mediated by membrane-bound glucocorticoid receptors (GRs). S-palmitoylation is a lipid post-translational modification that mediates the membrane localization of some steroid receptors. A highly homologous amino acid sequence (663YLCM KTLLL671) is present ...

Three Novel Heterozygous Point Mutations of NR3C1 causing Glucocorticoid Resistance
Vitellius G et al.
Generalized glucocorticoid resistance is associated with glucocorticoid receptor (GR, NR3C1) mutations. Three novel heterozygous missense NR3C1 mutations (R477S, Y478C and L672P) were identified in patients presenting with adrenal incidentalomas, glucocorticoid excess without Cushing syndrome. Dexamethasone (DXM) bi...

Functional characterization of the hGRαT556I causing Chrousos syndrome
Nicolaides NC et al.
BACKGROUND: Chrousos syndrome is a rare pathologic condition characterized by generalized, partial resistance of target tissues to glucocorticoids and caused by inactivating mutations of the human glucocorticoid receptor (hGR) gene. A novel case of Chrousos syndrome has been reported in a patient with adrenal incid...

Perinatal exposure to low-dose bisphenol A affects the neuroendocrine stress response in rats.
Panagiotidou E, Zerva S, Mitsiou DJ, Alexis MN, Kitraki E
Bisphenol A (BPA) is an estrogen-mimicking endocrine disruptor. Early-life exposures to low doses of BPA exert long-lasting effects on animals' reproductive and brain physiology. However, little is known about the effects of BPA on the stress-response system. Given the interaction of sex and stress hormones, we exam...

Pattern of heat shock factor and heat shock protein expression in lymphocytes of bipolar patients: Increased HSP70-glucocorticoid receptor heterocomplex.
Bei ES, Salpeas V, Alevizos B, Anagnostara C, Pappa D, Moutsatsou P
Bipolar disorder (BD), a stress-related disease, is characterized by altered glucocorticoid receptor (GR) signalling. Stress response includes activation of heat shock factor (HSF) and subsequent heat shock protein (HSP) synthesis which regulate GR folding and function. The objective of this study was to investigate...

Coactivation of GR and NFKB alters the repertoire of their binding sites and target genes.
Rao NA, McCalman MT, Moulos P, Francoijs KJ, Chatziioannou A, Kolisis FN, Alexis MN, Mitsiou DJ, Stunnenberg HG
Glucocorticoid receptor (GR) exerts anti-inflammatory action in part by antagonizing proinflammatory transcription factors such as the nuclear factor kappa-b (NFKB). Here, we assess the crosstalk of activated GR and RELA (p65, major NFKB component) by global identification of their binding sites and target genes. We...

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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP assays were performed using HeLa cells, the Diagenode monoclonal antibody directed against GR (Cat. No. MAb-010-050) and optimized PCR primer sets for qPCR. The cells were treated either with ethanol (EtOH, used as a negative control) or triamcinolone acetonide (TA) for 4 hours prior to cell harvesting. ChIP was performed using sheared chromatin from 3 million cells and 5 &mu;g of antibody. QPCR was performed with primers for the human metallothionein promoter (hMTIIA) and for exon 2 of the human myoglobin gene (hmyo ex2), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the human metallothionein IIA promoter by GR. </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP was performed on sheared chromatin from 3.5 million HeLaB2 cells using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). The cells were treated with the synthetic GR ligand triamcinolone acetonide (TA) for 4 hours prior to harvesting. The IP&rsquo;d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence of chromosome 16 (figure 2A) as well as the MT2A positive control gene (figure 2B). The position of the PCR amplicon is also indicated. Figure 2C, D and E show the results for the known GR target genes PER1 on chromosome 17 and FKBP5 and TNFAIP3 on chromosome 6. </small></p>
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<p><small><strong> Figure 3. Sandwich ELISA </strong><br />The specificity of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050) was assessed by sandwich ELISA. Figure 3A: schematic representation of the sandwich ELISA with the monoclonal antibody against hGR (clone #:m2F8). Figure 3B: ELISA results using the monoclonal antibody against hGR at a concentration of 0.5 &mu;g/ml. The figure shows an ELISA signal which is proportionally increasing with increasing amounts of recombinant hGR. </small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode monoclonal antibody against hGR </strong><br />Figure 4A. Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10e6 Raji or Molt cells were analysed by Western blot using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). Figure 4B. Western blot analysis of extracts from 300,000 HeLa cells with the Diagenode monoclonal antibody against hGR (concentration 1 &mu;g/ml). </small></p>
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<p><small><strong> Figure 5. Immunohistochemistry and immunofluorescence using the Diagenode monoclonal antibody against hGR </strong><br />1. Immunoreactivity of the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050) in rat CA1 neurons of hippocampus. The antibody was used at a concentration of 2.5 &mu;g/ml. <br />2. COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary antibody and peroxidase-labeled avidin. The antibody was used at a concentration of 2.5 &mu;g/ml. </small></p>
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<p><small><strong> Figure 6. Immunoprecipitation using the Diagenode monoclonal antibody against hGR </strong><br />The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 &mu;l IP reaction solution) using 5 &mu;g of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050). The IP was followed by Western blot analysis as described above </small></p>
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		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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		'modified' => '2020-09-18 16:34:22',
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		'select_label' => '326 - GR monoclonal antibody (NR-010 - 1.0 &micro;g/&micro;l - Human, rat - Ammonium sulphate purified - Mouse)'
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			'name' => 'GR Antibody',
			'description' => '<p>Alternative names: <strong>NR3C1</strong>,<strong> GCCR</strong>, <strong>GCR</strong>, <strong>GRL</strong></p>
<p>Monoclonal antibody raised in mouse against amino acids 304-428 of the human <strong>GR (glucocorticoid receptor)</strong>, using a chimeric protein.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig1.png" alt="GR Antibody ChIP Grade" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP assays were performed using HeLa cells, the Diagenode monoclonal antibody directed against GR (Cat. No. MAb-010-050) and optimized PCR primer sets for qPCR. The cells were treated either with ethanol (EtOH, used as a negative control) or triamcinolone acetonide (TA) for 4 hours prior to cell harvesting. ChIP was performed using sheared chromatin from 3 million cells and 5 &mu;g of antibody. QPCR was performed with primers for the human metallothionein promoter (hMTIIA) and for exon 2 of the human myoglobin gene (hmyo ex2), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the human metallothionein IIA promoter by GR. </small></p>
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</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2A.png" alt="GR Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2B.png" alt="GR Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2C.png" alt="GR Antibody for ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2D.png" alt="GR Antibody validated in ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2E.png" alt="GR Antibody ChIP-seq Grade" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP was performed on sheared chromatin from 3.5 million HeLaB2 cells using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). The cells were treated with the synthetic GR ligand triamcinolone acetonide (TA) for 4 hours prior to harvesting. The IP&rsquo;d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence of chromosome 16 (figure 2A) as well as the MT2A positive control gene (figure 2B). The position of the PCR amplicon is also indicated. Figure 2C, D and E show the results for the known GR target genes PER1 on chromosome 17 and FKBP5 and TNFAIP3 on chromosome 6. </small></p>
</div>
</div>
<p></p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig3.png" alt="GR Antibody ELISA Validation" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Sandwich ELISA </strong><br />The specificity of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050) was assessed by sandwich ELISA. Figure 3A: schematic representation of the sandwich ELISA with the monoclonal antibody against hGR (clone #:m2F8). Figure 3B: ELISA results using the monoclonal antibody against hGR at a concentration of 0.5 &mu;g/ml. The figure shows an ELISA signal which is proportionally increasing with increasing amounts of recombinant hGR. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig4.png" alt="GR Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode monoclonal antibody against hGR </strong><br />Figure 4A. Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10e6 Raji or Molt cells were analysed by Western blot using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). Figure 4B. Western blot analysis of extracts from 300,000 HeLa cells with the Diagenode monoclonal antibody against hGR (concentration 1 &mu;g/ml). </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig5.png" alt="GR Antibody validated in Immunohistochemistry" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig6.png" alt="GR Antibody validated for Immunohistochemistry" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Immunohistochemistry and immunofluorescence using the Diagenode monoclonal antibody against hGR </strong><br />1. Immunoreactivity of the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050) in rat CA1 neurons of hippocampus. The antibody was used at a concentration of 2.5 &mu;g/ml. <br />2. COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary antibody and peroxidase-labeled avidin. The antibody was used at a concentration of 2.5 &mu;g/ml. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig7.png" alt="GR Antibody validated in Immunoprecipitation" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 6. Immunoprecipitation using the Diagenode monoclonal antibody against hGR </strong><br />The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 &mu;l IP reaction solution) using 5 &mu;g of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050). The IP was followed by Western blot analysis as described above </small></p>
</div>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
			'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
			'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
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			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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			'description' => '<p><span style="font-weight: 400;">The list of Diagenode&rsquo;s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. &nbsp;Check the list below to see our targets.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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			'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie',
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			'name' => 'Antibodies you can trust',
			'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
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			'name' => 'Epigenetic Antibodies Brochure',
			'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
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			'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
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			'name' => 'Datasheet hGR C15200010',
			'description' => '<p>Datasheet description</p>',
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			'type' => 'Datasheet',
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			'alt' => 'ChIP-seq Grade',
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			'id' => '4253',
			'name' => 'Coordinated glucocorticoid receptor and MAFB action inducestolerogenesis and epigenome remodeling in dendritic cells',
			'authors' => 'Morante-Palacios Octavio et al.',
			'description' => '<p>Abstract Glucocorticoids (GCs) exert potent anti-inflammatory effects in immune cells through the glucocorticoid receptor (GR). Dendritic cells (DCs), central actors for coordinating immune responses, acquire tolerogenic properties in response to GCs. Tolerogenic DCs (tolDCs) have emerged as a potential treatment for various inflammatory diseases. To date, the underlying cell type-specific regulatory mechanisms orchestrating GC-mediated acquisition of immunosuppressive properties remain poorly understood. In this study, we investigated the transcriptomic and epigenomic remodeling associated with differentiation to DCs in the presence of GCs. Our analysis demonstrates a major role of MAFB in this process, in synergy with GR. GR and MAFB both interact with methylcytosine dioxygenase TET2 and bind to genomic loci that undergo specific demethylation in tolDCs. We also show that the role of MAFB is more extensive, binding to thousands of genomic loci in tolDCs. Finally, MAFB knockdown erases the tolerogenic properties of tolDCs and reverts the specific DNA demethylation and gene upregulation. The preeminent role of MAFB is also demonstrated in vivo for myeloid cells from synovium in rheumatoid arthritis following GC treatment. Our results imply that, once directly activated by GR, MAFB plays a critical role in orchestrating the epigenomic and transcriptomic remodeling that define the tolerogenic phenotype.</p>',
			'date' => '2022-01-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34893889',
			'doi' => '10.1093/nar/gkab1182',
			'modified' => '2022-05-20 09:44:29',
			'created' => '2022-05-19 10:41:50',
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		(int) 1 => array(
			'id' => '3208',
			'name' => 'The Role of S-Palmitoylation of the Human Glucocorticoid Receptor (hGR) in Mediating the Nongenomic Glucocorticoid Actions',
			'authors' => 'Nicolaides N. et al.',
			'description' => '<p>Background: Many rapid nongenomic glucocorticoid actions are mediated by membrane-bound glucocorticoid receptors (GRs). S-palmitoylation is a lipid post-translational modification that mediates the membrane localization of some steroid receptors. A highly homologous amino acid sequence (663YLCM KTLLL671) is present in the ligand-binding domain of hGR&alpha;, suggesting that hGR&alpha; might also undergo S-palmitoylation.</p>
<p>Aim: To investigate the role of the motif 663YLCMKTLLL671 in membrane localization of the hGR&alpha; and in mediating rapid nongenomic glucocorticoid signaling.</p>
<p>Methods and Results: We showed that the mutant receptors hGR&alpha;Y663A, hGR&alpha;C665A and hGR&alpha;LL670/671AA, and the addition of the palmitoylation inhibitor 2-bromopalmitate did not prevent membrane localization of hGR&alpha; and co-localization with caveolin-1, and did not influence the biphasic activation of mitogen-activated protein kinase (MAPK) signaling pathway in the early time points. Finally, the hGR&alpha; was not shown to undergo S-palmitoylation.</p>
<p>Conclusions: The motif 663YLCMKTLLL671 does not play a role in membrane localization of hGR&alpha; and does not mediate the nongenomic glucocorticoid actions.</p>',
			'date' => '2017-04-15',
			'pmid' => 'http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/199',
			'doi' => '',
			'modified' => '2017-07-07 16:21:23',
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		(int) 2 => array(
			'id' => '2923',
			'name' => 'Three Novel Heterozygous Point Mutations of NR3C1 causing Glucocorticoid Resistance',
			'authors' => 'Vitellius G et al.',
			'description' => '<p>Generalized glucocorticoid resistance is associated with glucocorticoid receptor (GR, NR3C1) mutations. Three novel heterozygous missense NR3C1 mutations (R477S, Y478C and L672P) were identified in patients presenting with adrenal incidentalomas, glucocorticoid excess without Cushing syndrome. Dexamethasone (DXM) binding studies demonstrated that the affinity of GR<sub>R477S</sub> and GR<sub>Y478C</sub> mutants, located in the DNA-binding domain of GR, was similar to wild-type GR (Kd = 2-3 nM). In contrast, GR<sub>L672P</sub> mutant, located in the ligand-binding domain of GR, was unable to bind glucocorticoids and was more sensitive to protein degradation. GR subcellular distribution revealed a marked decrease in DXM-induced nuclear translocation of GR<sub>R477S</sub> and GR<sub>Y478C</sub> mutants, whereas GR<sub>L672P</sub> remained exclusively cytoplasmic. Chromatin immunoprecipitation demonstrated impaired recruitment of DBD mutants onto the regulatory sequence of FKBP5. Transactivation assays disclosed the lack of transcriptional activity of GR<sub>R477S</sub> and GR<sub>L672P</sub> while GR<sub>Y478C</sub> had a reduced transactivation capacity. Three-D modeling indicated that R477S lost two essential hydrogen bonds with DNA, Y478C resulted in altered interaction with surrounding amino-acids, destabilizing DBD, while L672P altered the H8 helix folding, leading to unstructured LBD. This study identifies novel NR3C1 mutations with their molecular consequences on altered GR signaling and suggests that genetic screening of NR3C1 should be conducted in patients with subclinical hypercorticism.</p>',
			'date' => '2016-04-27',
			'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27120390',
			'doi' => '10.1002/humu.23008',
			'modified' => '2016-05-13 17:32:26',
			'created' => '2016-05-13 17:32:26',
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			'id' => '2967',
			'name' => 'Functional characterization of the hGRαT556I causing Chrousos syndrome',
			'authors' => 'Nicolaides NC et al.',
			'description' => '<h4>BACKGROUND:</h4>
<p><abstracttext label="BACKGROUND" nlmcategory="BACKGROUND">Chrousos syndrome is a rare pathologic condition characterized by generalized, partial resistance of target tissues to glucocorticoids and caused by inactivating mutations of the human glucocorticoid receptor (hGR) gene. A novel case of Chrousos syndrome has been reported in a patient with adrenal incidentaloma, who harboured a heterozygous point mutation in the hGR gene, which resulted in threonine (T) to isoleucine (I) substitution at amino acid position 556 in the ligand-binding domain of the receptor.</abstracttext></p>
<h4>OBJECTIVE:</h4>
<p><abstracttext label="OBJECTIVE" nlmcategory="OBJECTIVE">To delineate the molecular mechanisms through which the mutant receptor hGR&alpha;T556I causes Chrousos syndrome.</abstracttext></p>
<h4>DESIGN AND RESULTS:</h4>
<p><abstracttext label="DESIGN AND RESULTS" nlmcategory="RESULTS">Compared with the wild-type receptor, the mutant receptor hGR&alpha;T556I demonstrated 50% reduction in its ability to transactivate glucocorticoid-responsive genes and in the affinity for the ligand, 30% increase in the ability to transrepress the nuclear factor-&kappa;B-target genes and a 3,4-fold delay in the cytoplasmic-to-nuclear translocation. The mutant receptor hGR&alpha;T556I did not exert a dominant negative effect upon the hGR&alpha;-mediated transcriptional activity; it preserved its ability to bind to DNA and interacted with the glucocorticoid receptor-interacting protein 1 coactivator mostly through its activation function-1 domain. Structural biology studies revealed that the T556I mutation caused disruption of the hydrogen bond formed by the T556 with the =O group of P637 backbone, which resulted in a significant relocation of the P637-bearing loop. This conformational alteration affected the local 3D arrangement of the receptor and hence the electrostatic surface of the region.</abstracttext></p>
<h4>CONCLUSIONS:</h4>
<p><abstracttext label="CONCLUSIONS" nlmcategory="CONCLUSIONS">The hGR&alpha;T556I causes Chrousos syndrome by impairing multiple steps of the glucocorticoid signal transduction pathway.</abstracttext></p>',
			'date' => '2016-01-01',
			'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26541474',
			'doi' => ' 10.1111/eci.12563',
			'modified' => '2016-06-29 09:41:42',
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		(int) 4 => array(
			'id' => '1776',
			'name' => 'Perinatal exposure to low-dose bisphenol A affects the neuroendocrine stress response in rats.',
			'authors' => 'Panagiotidou E, Zerva S, Mitsiou DJ, Alexis MN, Kitraki E',
			'description' => 'Bisphenol A (BPA) is an estrogen-mimicking endocrine disruptor. Early-life exposures to low doses of BPA exert long-lasting effects on animals' reproductive and brain physiology. However, little is known about the effects of BPA on the stress-response system. Given the interaction of sex and stress hormones, we examined the effect of a low perinatal BPA exposure on the function of the hypothalamic-pituitary-adrenal (HPA) axis at rest and upon application of acute stress. Throughout pregnancy and lactation rats received daily 40 μg BPA/kg body weight orally via cornflakes. We studied the effect of this low but chronic exposure to BPA in the male and female offspring at puberty. BPA exposure led to abnormal adrenal histology including reduced zona reticularis especially in male offspring, hyperplasia of zona fasciculata in both sexes, and increased adrenal weight in female offspring. BPA-treated females had increased basal corticosterone and reduced hypothalamic glucocorticoid receptors (GR) levels. Stressed BPA-exposed females exhibited anxiety-like behavioral coping, a less rigorous corticosterone response, and did not downregulate GR in the hypothalamus, compared with control females. BPA-exposed males exhibited a heightened corticosterone stress response compared with females; they also displayed increased pro-opiomelanocortin mRNA levels and retained the prestress levels of pituitary corticotropin-releasing hormone-receptor 1, compared with control males. We found that perinatal chronic exposure to a low dose of BPA perturbs the basal and stress-induced activity of the HPA axis in a sexually dimorphic manner at adolescence. Exposure to BPA might contribute to increased susceptibility to stress-related disorders in later life.',
			'date' => '2014-03-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/24323913',
			'doi' => '',
			'modified' => '2015-07-24 15:39:01',
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			'id' => '1557',
			'name' => 'Pattern of heat shock factor and heat shock protein expression in lymphocytes of bipolar patients: Increased HSP70-glucocorticoid receptor heterocomplex.',
			'authors' => 'Bei ES, Salpeas V, Alevizos B, Anagnostara C, Pappa D, Moutsatsou P',
			'description' => 'Bipolar disorder (BD), a stress-related disease, is characterized by altered glucocorticoid receptor (GR) signalling. Stress response includes activation of heat shock factor (HSF) and subsequent heat shock protein (HSP) synthesis which regulate GR folding and function. The objective of this study was to investigate the possible role of HSFs, HSPs and their interaction with GR in BD. We applied immunoprecipitation, SDS-PAGE/Western blot analysis and electrophoretic mobility shift assay (EMSA) in lymphocytes (whole cell or nuclear extracts) from BD patients and healthy subjects and determined the HSPs (HSP90 and HSP70), the heterocomplexes HSP90-GR and HSP70-GR, the HSFs (HSF1 and HSF4) as well as the HSF-DNA binding. The HSP70-GR heterocomplex was elevated (p < 0.05) in BD patients vs healthy subjects, and nuclear HSP70 was reduced (p ≤ 0.01) in bipolar manic patients. Protein levels of HSF1, HSF4, HSP90, HSP90-GR heterocomplex, and HSF-DNA binding remained unaltered in BD patients vs healthy subjects. The corresponding effect sizes (ES) indicated a large ES for HSP70-GR, HSP70, HSF-DNA binding and HSF4, and a medium ES for HSP90, HSF1 and HSP90-GR between healthy subjects and bipolar patients. Significant correlations among HSFs, HSPs, GR and HSP70-GR heterocomplex were observed in healthy subjects, which were abrogated in bipolar patients. The higher interaction between GR and HSP70 and the disturbances in the relations among heat shock response parameters and GR as observed in our BD patients may provide novel insights into the contribution of these factors in BD aetiopathogenesis.',
			'date' => '2013-11-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/23938235',
			'doi' => '',
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			'name' => 'Coactivation of GR and NFKB alters the repertoire of their binding sites and target genes.',
			'authors' => 'Rao NA, McCalman MT, Moulos P, Francoijs KJ, Chatziioannou A, Kolisis FN, Alexis MN, Mitsiou DJ, Stunnenberg HG',
			'description' => 'Glucocorticoid receptor (GR) exerts anti-inflammatory action in part by antagonizing proinflammatory transcription factors such as the nuclear factor kappa-b (NFKB). Here, we assess the crosstalk of activated GR and RELA (p65, major NFKB component) by global identification of their binding sites and target genes. We show that coactivation of GR and p65 alters the repertoire of regulated genes and results in their association with novel sites in a mutually dependent manner. These novel sites predominantly cluster with p65 target genes that are antagonized by activated GR and vice versa. Our data show that coactivation of GR and NFKB alters signaling pathways that are regulated by each factor separately and provide insight into the networks underlying the GR and NFKB crosstalk.',
			'date' => '2011-09-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/21750107',
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		'id' => '1970',
		'antibody_id' => '326',
		'name' => 'GR Antibody',
		'description' => '<p>Alternative names: <strong>NR3C1</strong>,<strong> GCCR</strong>, <strong>GCR</strong>, <strong>GRL</strong></p>
<p>Monoclonal antibody raised in mouse against amino acids 304-428 of the human <strong>GR (glucocorticoid receptor)</strong>, using a chimeric protein.</p>',
		'label1' => 'Validation Data',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig1.png" alt="GR Antibody ChIP Grade" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP assays were performed using HeLa cells, the Diagenode monoclonal antibody directed against GR (Cat. No. MAb-010-050) and optimized PCR primer sets for qPCR. The cells were treated either with ethanol (EtOH, used as a negative control) or triamcinolone acetonide (TA) for 4 hours prior to cell harvesting. ChIP was performed using sheared chromatin from 3 million cells and 5 &mu;g of antibody. QPCR was performed with primers for the human metallothionein promoter (hMTIIA) and for exon 2 of the human myoglobin gene (hmyo ex2), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the human metallothionein IIA promoter by GR. </small></p>
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<div class="row">
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2A.png" alt="GR Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2B.png" alt="GR Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2C.png" alt="GR Antibody for ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2D.png" alt="GR Antibody validated in ChIP-seq" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP was performed on sheared chromatin from 3.5 million HeLaB2 cells using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). The cells were treated with the synthetic GR ligand triamcinolone acetonide (TA) for 4 hours prior to harvesting. The IP&rsquo;d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence of chromosome 16 (figure 2A) as well as the MT2A positive control gene (figure 2B). The position of the PCR amplicon is also indicated. Figure 2C, D and E show the results for the known GR target genes PER1 on chromosome 17 and FKBP5 and TNFAIP3 on chromosome 6. </small></p>
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<p></p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig3.png" alt="GR Antibody ELISA Validation" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Sandwich ELISA </strong><br />The specificity of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050) was assessed by sandwich ELISA. Figure 3A: schematic representation of the sandwich ELISA with the monoclonal antibody against hGR (clone #:m2F8). Figure 3B: ELISA results using the monoclonal antibody against hGR at a concentration of 0.5 &mu;g/ml. The figure shows an ELISA signal which is proportionally increasing with increasing amounts of recombinant hGR. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig4.png" alt="GR Antibody validated in Western Blot" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode monoclonal antibody against hGR </strong><br />Figure 4A. Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10e6 Raji or Molt cells were analysed by Western blot using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). Figure 4B. Western blot analysis of extracts from 300,000 HeLa cells with the Diagenode monoclonal antibody against hGR (concentration 1 &mu;g/ml). </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig5.png" alt="GR Antibody validated in Immunohistochemistry" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig6.png" alt="GR Antibody validated for Immunohistochemistry" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Immunohistochemistry and immunofluorescence using the Diagenode monoclonal antibody against hGR </strong><br />1. Immunoreactivity of the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050) in rat CA1 neurons of hippocampus. The antibody was used at a concentration of 2.5 &mu;g/ml. <br />2. COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary antibody and peroxidase-labeled avidin. The antibody was used at a concentration of 2.5 &mu;g/ml. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig7.png" alt="GR Antibody validated in Immunoprecipitation" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 6. Immunoprecipitation using the Diagenode monoclonal antibody against hGR </strong><br />The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 &mu;l IP reaction solution) using 5 &mu;g of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050). The IP was followed by Western blot analysis as described above </small></p>
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	'meta_description' => 'GR (Glucocorticoid receptor) Monoclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB, IP, and ELISA. Batch-specific data available on the website. Alternative names: NR3C1, GCCR, GCR, GRL. Sample size available.',
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	'name' => 'GR Antibody',
	'description' => '<p>Alternative names: <strong>NR3C1</strong>,<strong> GCCR</strong>, <strong>GCR</strong>, <strong>GRL</strong></p>
<p>Monoclonal antibody raised in mouse against amino acids 304-428 of the human <strong>GR (glucocorticoid receptor)</strong>, using a chimeric protein.</p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig1.png" alt="GR Antibody ChIP Grade" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP assays were performed using HeLa cells, the Diagenode monoclonal antibody directed against GR (Cat. No. MAb-010-050) and optimized PCR primer sets for qPCR. The cells were treated either with ethanol (EtOH, used as a negative control) or triamcinolone acetonide (TA) for 4 hours prior to cell harvesting. ChIP was performed using sheared chromatin from 3 million cells and 5 &mu;g of antibody. QPCR was performed with primers for the human metallothionein promoter (hMTIIA) and for exon 2 of the human myoglobin gene (hmyo ex2), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the human metallothionein IIA promoter by GR. </small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2A.png" alt="GR Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2B.png" alt="GR Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2C.png" alt="GR Antibody for ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2D.png" alt="GR Antibody validated in ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2E.png" alt="GR Antibody ChIP-seq Grade" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP was performed on sheared chromatin from 3.5 million HeLaB2 cells using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). The cells were treated with the synthetic GR ligand triamcinolone acetonide (TA) for 4 hours prior to harvesting. The IP&rsquo;d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence of chromosome 16 (figure 2A) as well as the MT2A positive control gene (figure 2B). The position of the PCR amplicon is also indicated. Figure 2C, D and E show the results for the known GR target genes PER1 on chromosome 17 and FKBP5 and TNFAIP3 on chromosome 6. </small></p>
</div>
</div>
<p></p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig3.png" alt="GR Antibody ELISA Validation" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Sandwich ELISA </strong><br />The specificity of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050) was assessed by sandwich ELISA. Figure 3A: schematic representation of the sandwich ELISA with the monoclonal antibody against hGR (clone #:m2F8). Figure 3B: ELISA results using the monoclonal antibody against hGR at a concentration of 0.5 &mu;g/ml. The figure shows an ELISA signal which is proportionally increasing with increasing amounts of recombinant hGR. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig4.png" alt="GR Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode monoclonal antibody against hGR </strong><br />Figure 4A. Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10e6 Raji or Molt cells were analysed by Western blot using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). Figure 4B. Western blot analysis of extracts from 300,000 HeLa cells with the Diagenode monoclonal antibody against hGR (concentration 1 &mu;g/ml). </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig5.png" alt="GR Antibody validated in Immunohistochemistry" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig6.png" alt="GR Antibody validated for Immunohistochemistry" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Immunohistochemistry and immunofluorescence using the Diagenode monoclonal antibody against hGR </strong><br />1. Immunoreactivity of the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050) in rat CA1 neurons of hippocampus. The antibody was used at a concentration of 2.5 &mu;g/ml. <br />2. COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary antibody and peroxidase-labeled avidin. The antibody was used at a concentration of 2.5 &mu;g/ml. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig7.png" alt="GR Antibody validated in Immunoprecipitation" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 6. Immunoprecipitation using the Diagenode monoclonal antibody against hGR </strong><br />The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 &mu;l IP reaction solution) using 5 &mu;g of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050). The IP was followed by Western blot analysis as described above </small></p>
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	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Flow cytometry applications',
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	'created' => '2015-07-08 13:53:00',
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	'authors' => 'Rao NA, McCalman MT, Moulos P, Francoijs KJ, Chatziioannou A, Kolisis FN, Alexis MN, Mitsiou DJ, Stunnenberg HG',
	'description' => 'Glucocorticoid receptor (GR) exerts anti-inflammatory action in part by antagonizing proinflammatory transcription factors such as the nuclear factor kappa-b (NFKB). Here, we assess the crosstalk of activated GR and RELA (p65, major NFKB component) by global identification of their binding sites and target genes. We show that coactivation of GR and p65 alters the repertoire of regulated genes and results in their association with novel sites in a mutually dependent manner. These novel sites predominantly cluster with p65 target genes that are antagonized by activated GR and vice versa. Our data show that coactivation of GR and NFKB alters signaling pathways that are regulated by each factor separately and provide insight into the networks underlying the GR and NFKB crosstalk.',
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	'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/21750107',
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include - APP/View/Products/view.ctp, line 755
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View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
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<th>Suggested dilution</th>
<th>References</th>
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<td>5 &mu;g/ChIP</td>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP assays were performed using HeLa cells, the Diagenode monoclonal antibody directed against GR (Cat. No. MAb-010-050) and optimized PCR primer sets for qPCR. The cells were treated either with ethanol (EtOH, used as a negative control) or triamcinolone acetonide (TA) for 4 hours prior to cell harvesting. ChIP was performed using sheared chromatin from 3 million cells and 5 &mu;g of antibody. QPCR was performed with primers for the human metallothionein promoter (hMTIIA) and for exon 2 of the human myoglobin gene (hmyo ex2), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the human metallothionein IIA promoter by GR. </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP was performed on sheared chromatin from 3.5 million HeLaB2 cells using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). The cells were treated with the synthetic GR ligand triamcinolone acetonide (TA) for 4 hours prior to harvesting. The IP&rsquo;d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence of chromosome 16 (figure 2A) as well as the MT2A positive control gene (figure 2B). The position of the PCR amplicon is also indicated. Figure 2C, D and E show the results for the known GR target genes PER1 on chromosome 17 and FKBP5 and TNFAIP3 on chromosome 6. </small></p>
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<p><small><strong> Figure 3. Sandwich ELISA </strong><br />The specificity of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050) was assessed by sandwich ELISA. Figure 3A: schematic representation of the sandwich ELISA with the monoclonal antibody against hGR (clone #:m2F8). Figure 3B: ELISA results using the monoclonal antibody against hGR at a concentration of 0.5 &mu;g/ml. The figure shows an ELISA signal which is proportionally increasing with increasing amounts of recombinant hGR. </small></p>
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<p><small><strong> Figure 5. Immunohistochemistry and immunofluorescence using the Diagenode monoclonal antibody against hGR </strong><br />1. Immunoreactivity of the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050) in rat CA1 neurons of hippocampus. The antibody was used at a concentration of 2.5 &mu;g/ml. <br />2. COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary antibody and peroxidase-labeled avidin. The antibody was used at a concentration of 2.5 &mu;g/ml. </small></p>
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<p><small><strong> Figure 6. Immunoprecipitation using the Diagenode monoclonal antibody against hGR </strong><br />The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 &mu;l IP reaction solution) using 5 &mu;g of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050). The IP was followed by Western blot analysis as described above </small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP assays were performed using HeLa cells, the Diagenode monoclonal antibody directed against GR (Cat. No. MAb-010-050) and optimized PCR primer sets for qPCR. The cells were treated either with ethanol (EtOH, used as a negative control) or triamcinolone acetonide (TA) for 4 hours prior to cell harvesting. ChIP was performed using sheared chromatin from 3 million cells and 5 &mu;g of antibody. QPCR was performed with primers for the human metallothionein promoter (hMTIIA) and for exon 2 of the human myoglobin gene (hmyo ex2), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the human metallothionein IIA promoter by GR. </small></p>
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2A.png" alt="GR Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2B.png" alt="GR Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2C.png" alt="GR Antibody for ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2D.png" alt="GR Antibody validated in ChIP-seq" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP was performed on sheared chromatin from 3.5 million HeLaB2 cells using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). The cells were treated with the synthetic GR ligand triamcinolone acetonide (TA) for 4 hours prior to harvesting. The IP&rsquo;d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence of chromosome 16 (figure 2A) as well as the MT2A positive control gene (figure 2B). The position of the PCR amplicon is also indicated. Figure 2C, D and E show the results for the known GR target genes PER1 on chromosome 17 and FKBP5 and TNFAIP3 on chromosome 6. </small></p>
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<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig3.png" alt="GR Antibody ELISA Validation" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 3. Sandwich ELISA </strong><br />The specificity of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050) was assessed by sandwich ELISA. Figure 3A: schematic representation of the sandwich ELISA with the monoclonal antibody against hGR (clone #:m2F8). Figure 3B: ELISA results using the monoclonal antibody against hGR at a concentration of 0.5 &mu;g/ml. The figure shows an ELISA signal which is proportionally increasing with increasing amounts of recombinant hGR. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig4.png" alt="GR Antibody validated in Western Blot" /></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode monoclonal antibody against hGR </strong><br />Figure 4A. Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10e6 Raji or Molt cells were analysed by Western blot using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). Figure 4B. Western blot analysis of extracts from 300,000 HeLa cells with the Diagenode monoclonal antibody against hGR (concentration 1 &mu;g/ml). </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig5.png" alt="GR Antibody validated in Immunohistochemistry" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig6.png" alt="GR Antibody validated for Immunohistochemistry" /></p>
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<p><small><strong> Figure 5. Immunohistochemistry and immunofluorescence using the Diagenode monoclonal antibody against hGR </strong><br />1. Immunoreactivity of the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050) in rat CA1 neurons of hippocampus. The antibody was used at a concentration of 2.5 &mu;g/ml. <br />2. COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary antibody and peroxidase-labeled avidin. The antibody was used at a concentration of 2.5 &mu;g/ml. </small></p>
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<div class="row">
<div class="small-4 columns">
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<div class="small-8 columns">
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			'name' => 'ELISA',
			'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
</div>',
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'description' => '<p>Immunoprecipitation</p>',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
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			'name' => 'GSA',
			'description' => '<p><span>Gel Supershift</span></p>',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Gel Supershift applications',
			'meta_title' => 'Gel Shift/Gel Super Shift Assays - Monoclonal antibody  | Diagenode',
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			'name' => 'ChIP-grade antibodies',
			'description' => '<div class="row">
<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
			'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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			'name' => 'Sample size antibodies',
			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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			'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
			'meta_title' => 'Sample-size Antibodies | Diagenode',
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			'name' => 'Transcription',
			'description' => '<p><span style="font-weight: 400;">The list of Diagenode&rsquo;s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. &nbsp;Check the list below to see our targets.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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			'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie',
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			'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
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			'id' => '38',
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			'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
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			'name' => 'Datasheet hGR C15200010',
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			'url' => 'files/products/antibodies/Datasheet_hGR_C15200010.pdf',
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			'name' => 'Coordinated glucocorticoid receptor and MAFB action inducestolerogenesis and epigenome remodeling in dendritic cells',
			'authors' => 'Morante-Palacios Octavio et al.',
			'description' => '<p>Abstract Glucocorticoids (GCs) exert potent anti-inflammatory effects in immune cells through the glucocorticoid receptor (GR). Dendritic cells (DCs), central actors for coordinating immune responses, acquire tolerogenic properties in response to GCs. Tolerogenic DCs (tolDCs) have emerged as a potential treatment for various inflammatory diseases. To date, the underlying cell type-specific regulatory mechanisms orchestrating GC-mediated acquisition of immunosuppressive properties remain poorly understood. In this study, we investigated the transcriptomic and epigenomic remodeling associated with differentiation to DCs in the presence of GCs. Our analysis demonstrates a major role of MAFB in this process, in synergy with GR. GR and MAFB both interact with methylcytosine dioxygenase TET2 and bind to genomic loci that undergo specific demethylation in tolDCs. We also show that the role of MAFB is more extensive, binding to thousands of genomic loci in tolDCs. Finally, MAFB knockdown erases the tolerogenic properties of tolDCs and reverts the specific DNA demethylation and gene upregulation. The preeminent role of MAFB is also demonstrated in vivo for myeloid cells from synovium in rheumatoid arthritis following GC treatment. Our results imply that, once directly activated by GR, MAFB plays a critical role in orchestrating the epigenomic and transcriptomic remodeling that define the tolerogenic phenotype.</p>',
			'date' => '2022-01-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34893889',
			'doi' => '10.1093/nar/gkab1182',
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		(int) 1 => array(
			'id' => '3208',
			'name' => 'The Role of S-Palmitoylation of the Human Glucocorticoid Receptor (hGR) in Mediating the Nongenomic Glucocorticoid Actions',
			'authors' => 'Nicolaides N. et al.',
			'description' => '<p>Background: Many rapid nongenomic glucocorticoid actions are mediated by membrane-bound glucocorticoid receptors (GRs). S-palmitoylation is a lipid post-translational modification that mediates the membrane localization of some steroid receptors. A highly homologous amino acid sequence (663YLCM KTLLL671) is present in the ligand-binding domain of hGR&alpha;, suggesting that hGR&alpha; might also undergo S-palmitoylation.</p>
<p>Aim: To investigate the role of the motif 663YLCMKTLLL671 in membrane localization of the hGR&alpha; and in mediating rapid nongenomic glucocorticoid signaling.</p>
<p>Methods and Results: We showed that the mutant receptors hGR&alpha;Y663A, hGR&alpha;C665A and hGR&alpha;LL670/671AA, and the addition of the palmitoylation inhibitor 2-bromopalmitate did not prevent membrane localization of hGR&alpha; and co-localization with caveolin-1, and did not influence the biphasic activation of mitogen-activated protein kinase (MAPK) signaling pathway in the early time points. Finally, the hGR&alpha; was not shown to undergo S-palmitoylation.</p>
<p>Conclusions: The motif 663YLCMKTLLL671 does not play a role in membrane localization of hGR&alpha; and does not mediate the nongenomic glucocorticoid actions.</p>',
			'date' => '2017-04-15',
			'pmid' => 'http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/199',
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		),
		(int) 2 => array(
			'id' => '2923',
			'name' => 'Three Novel Heterozygous Point Mutations of NR3C1 causing Glucocorticoid Resistance',
			'authors' => 'Vitellius G et al.',
			'description' => '<p>Generalized glucocorticoid resistance is associated with glucocorticoid receptor (GR, NR3C1) mutations. Three novel heterozygous missense NR3C1 mutations (R477S, Y478C and L672P) were identified in patients presenting with adrenal incidentalomas, glucocorticoid excess without Cushing syndrome. Dexamethasone (DXM) binding studies demonstrated that the affinity of GR<sub>R477S</sub> and GR<sub>Y478C</sub> mutants, located in the DNA-binding domain of GR, was similar to wild-type GR (Kd = 2-3 nM). In contrast, GR<sub>L672P</sub> mutant, located in the ligand-binding domain of GR, was unable to bind glucocorticoids and was more sensitive to protein degradation. GR subcellular distribution revealed a marked decrease in DXM-induced nuclear translocation of GR<sub>R477S</sub> and GR<sub>Y478C</sub> mutants, whereas GR<sub>L672P</sub> remained exclusively cytoplasmic. Chromatin immunoprecipitation demonstrated impaired recruitment of DBD mutants onto the regulatory sequence of FKBP5. Transactivation assays disclosed the lack of transcriptional activity of GR<sub>R477S</sub> and GR<sub>L672P</sub> while GR<sub>Y478C</sub> had a reduced transactivation capacity. Three-D modeling indicated that R477S lost two essential hydrogen bonds with DNA, Y478C resulted in altered interaction with surrounding amino-acids, destabilizing DBD, while L672P altered the H8 helix folding, leading to unstructured LBD. This study identifies novel NR3C1 mutations with their molecular consequences on altered GR signaling and suggests that genetic screening of NR3C1 should be conducted in patients with subclinical hypercorticism.</p>',
			'date' => '2016-04-27',
			'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27120390',
			'doi' => '10.1002/humu.23008',
			'modified' => '2016-05-13 17:32:26',
			'created' => '2016-05-13 17:32:26',
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		),
		(int) 3 => array(
			'id' => '2967',
			'name' => 'Functional characterization of the hGRαT556I causing Chrousos syndrome',
			'authors' => 'Nicolaides NC et al.',
			'description' => '<h4>BACKGROUND:</h4>
<p><abstracttext label="BACKGROUND" nlmcategory="BACKGROUND">Chrousos syndrome is a rare pathologic condition characterized by generalized, partial resistance of target tissues to glucocorticoids and caused by inactivating mutations of the human glucocorticoid receptor (hGR) gene. A novel case of Chrousos syndrome has been reported in a patient with adrenal incidentaloma, who harboured a heterozygous point mutation in the hGR gene, which resulted in threonine (T) to isoleucine (I) substitution at amino acid position 556 in the ligand-binding domain of the receptor.</abstracttext></p>
<h4>OBJECTIVE:</h4>
<p><abstracttext label="OBJECTIVE" nlmcategory="OBJECTIVE">To delineate the molecular mechanisms through which the mutant receptor hGR&alpha;T556I causes Chrousos syndrome.</abstracttext></p>
<h4>DESIGN AND RESULTS:</h4>
<p><abstracttext label="DESIGN AND RESULTS" nlmcategory="RESULTS">Compared with the wild-type receptor, the mutant receptor hGR&alpha;T556I demonstrated 50% reduction in its ability to transactivate glucocorticoid-responsive genes and in the affinity for the ligand, 30% increase in the ability to transrepress the nuclear factor-&kappa;B-target genes and a 3,4-fold delay in the cytoplasmic-to-nuclear translocation. The mutant receptor hGR&alpha;T556I did not exert a dominant negative effect upon the hGR&alpha;-mediated transcriptional activity; it preserved its ability to bind to DNA and interacted with the glucocorticoid receptor-interacting protein 1 coactivator mostly through its activation function-1 domain. Structural biology studies revealed that the T556I mutation caused disruption of the hydrogen bond formed by the T556 with the =O group of P637 backbone, which resulted in a significant relocation of the P637-bearing loop. This conformational alteration affected the local 3D arrangement of the receptor and hence the electrostatic surface of the region.</abstracttext></p>
<h4>CONCLUSIONS:</h4>
<p><abstracttext label="CONCLUSIONS" nlmcategory="CONCLUSIONS">The hGR&alpha;T556I causes Chrousos syndrome by impairing multiple steps of the glucocorticoid signal transduction pathway.</abstracttext></p>',
			'date' => '2016-01-01',
			'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26541474',
			'doi' => ' 10.1111/eci.12563',
			'modified' => '2016-06-29 09:41:42',
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			'name' => 'Perinatal exposure to low-dose bisphenol A affects the neuroendocrine stress response in rats.',
			'authors' => 'Panagiotidou E, Zerva S, Mitsiou DJ, Alexis MN, Kitraki E',
			'description' => 'Bisphenol A (BPA) is an estrogen-mimicking endocrine disruptor. Early-life exposures to low doses of BPA exert long-lasting effects on animals' reproductive and brain physiology. However, little is known about the effects of BPA on the stress-response system. Given the interaction of sex and stress hormones, we examined the effect of a low perinatal BPA exposure on the function of the hypothalamic-pituitary-adrenal (HPA) axis at rest and upon application of acute stress. Throughout pregnancy and lactation rats received daily 40 μg BPA/kg body weight orally via cornflakes. We studied the effect of this low but chronic exposure to BPA in the male and female offspring at puberty. BPA exposure led to abnormal adrenal histology including reduced zona reticularis especially in male offspring, hyperplasia of zona fasciculata in both sexes, and increased adrenal weight in female offspring. BPA-treated females had increased basal corticosterone and reduced hypothalamic glucocorticoid receptors (GR) levels. Stressed BPA-exposed females exhibited anxiety-like behavioral coping, a less rigorous corticosterone response, and did not downregulate GR in the hypothalamus, compared with control females. BPA-exposed males exhibited a heightened corticosterone stress response compared with females; they also displayed increased pro-opiomelanocortin mRNA levels and retained the prestress levels of pituitary corticotropin-releasing hormone-receptor 1, compared with control males. We found that perinatal chronic exposure to a low dose of BPA perturbs the basal and stress-induced activity of the HPA axis in a sexually dimorphic manner at adolescence. Exposure to BPA might contribute to increased susceptibility to stress-related disorders in later life.',
			'date' => '2014-03-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/24323913',
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			'name' => 'Pattern of heat shock factor and heat shock protein expression in lymphocytes of bipolar patients: Increased HSP70-glucocorticoid receptor heterocomplex.',
			'authors' => 'Bei ES, Salpeas V, Alevizos B, Anagnostara C, Pappa D, Moutsatsou P',
			'description' => 'Bipolar disorder (BD), a stress-related disease, is characterized by altered glucocorticoid receptor (GR) signalling. Stress response includes activation of heat shock factor (HSF) and subsequent heat shock protein (HSP) synthesis which regulate GR folding and function. The objective of this study was to investigate the possible role of HSFs, HSPs and their interaction with GR in BD. We applied immunoprecipitation, SDS-PAGE/Western blot analysis and electrophoretic mobility shift assay (EMSA) in lymphocytes (whole cell or nuclear extracts) from BD patients and healthy subjects and determined the HSPs (HSP90 and HSP70), the heterocomplexes HSP90-GR and HSP70-GR, the HSFs (HSF1 and HSF4) as well as the HSF-DNA binding. The HSP70-GR heterocomplex was elevated (p < 0.05) in BD patients vs healthy subjects, and nuclear HSP70 was reduced (p ≤ 0.01) in bipolar manic patients. Protein levels of HSF1, HSF4, HSP90, HSP90-GR heterocomplex, and HSF-DNA binding remained unaltered in BD patients vs healthy subjects. The corresponding effect sizes (ES) indicated a large ES for HSP70-GR, HSP70, HSF-DNA binding and HSF4, and a medium ES for HSP90, HSF1 and HSP90-GR between healthy subjects and bipolar patients. Significant correlations among HSFs, HSPs, GR and HSP70-GR heterocomplex were observed in healthy subjects, which were abrogated in bipolar patients. The higher interaction between GR and HSP70 and the disturbances in the relations among heat shock response parameters and GR as observed in our BD patients may provide novel insights into the contribution of these factors in BD aetiopathogenesis.',
			'date' => '2013-11-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/23938235',
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			'authors' => 'Rao NA, McCalman MT, Moulos P, Francoijs KJ, Chatziioannou A, Kolisis FN, Alexis MN, Mitsiou DJ, Stunnenberg HG',
			'description' => 'Glucocorticoid receptor (GR) exerts anti-inflammatory action in part by antagonizing proinflammatory transcription factors such as the nuclear factor kappa-b (NFKB). Here, we assess the crosstalk of activated GR and RELA (p65, major NFKB component) by global identification of their binding sites and target genes. We show that coactivation of GR and p65 alters the repertoire of regulated genes and results in their association with novel sites in a mutually dependent manner. These novel sites predominantly cluster with p65 target genes that are antagonized by activated GR and vice versa. Our data show that coactivation of GR and NFKB alters signaling pathways that are regulated by each factor separately and provide insight into the networks underlying the GR and NFKB crosstalk.',
			'date' => '2011-09-01',
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		'name' => 'GR Antibody',
		'description' => '<p>Alternative names: <strong>NR3C1</strong>,<strong> GCCR</strong>, <strong>GCR</strong>, <strong>GRL</strong></p>
<p>Monoclonal antibody raised in mouse against amino acids 304-428 of the human <strong>GR (glucocorticoid receptor)</strong>, using a chimeric protein.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP assays were performed using HeLa cells, the Diagenode monoclonal antibody directed against GR (Cat. No. MAb-010-050) and optimized PCR primer sets for qPCR. The cells were treated either with ethanol (EtOH, used as a negative control) or triamcinolone acetonide (TA) for 4 hours prior to cell harvesting. ChIP was performed using sheared chromatin from 3 million cells and 5 &mu;g of antibody. QPCR was performed with primers for the human metallothionein promoter (hMTIIA) and for exon 2 of the human myoglobin gene (hmyo ex2), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the human metallothionein IIA promoter by GR. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2A.png" alt="GR Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2B.png" alt="GR Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2C.png" alt="GR Antibody for ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2D.png" alt="GR Antibody validated in ChIP-seq" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP was performed on sheared chromatin from 3.5 million HeLaB2 cells using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). The cells were treated with the synthetic GR ligand triamcinolone acetonide (TA) for 4 hours prior to harvesting. The IP&rsquo;d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence of chromosome 16 (figure 2A) as well as the MT2A positive control gene (figure 2B). The position of the PCR amplicon is also indicated. Figure 2C, D and E show the results for the known GR target genes PER1 on chromosome 17 and FKBP5 and TNFAIP3 on chromosome 6. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig3.png" alt="GR Antibody ELISA Validation" /></p>
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<p><small><strong> Figure 3. Sandwich ELISA </strong><br />The specificity of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050) was assessed by sandwich ELISA. Figure 3A: schematic representation of the sandwich ELISA with the monoclonal antibody against hGR (clone #:m2F8). Figure 3B: ELISA results using the monoclonal antibody against hGR at a concentration of 0.5 &mu;g/ml. The figure shows an ELISA signal which is proportionally increasing with increasing amounts of recombinant hGR. </small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode monoclonal antibody against hGR </strong><br />Figure 4A. Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10e6 Raji or Molt cells were analysed by Western blot using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). Figure 4B. Western blot analysis of extracts from 300,000 HeLa cells with the Diagenode monoclonal antibody against hGR (concentration 1 &mu;g/ml). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig6.png" alt="GR Antibody validated for Immunohistochemistry" /></p>
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<p><small><strong> Figure 5. Immunohistochemistry and immunofluorescence using the Diagenode monoclonal antibody against hGR </strong><br />1. Immunoreactivity of the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050) in rat CA1 neurons of hippocampus. The antibody was used at a concentration of 2.5 &mu;g/ml. <br />2. COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary antibody and peroxidase-labeled avidin. The antibody was used at a concentration of 2.5 &mu;g/ml. </small></p>
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<p><small><strong> Figure 6. Immunoprecipitation using the Diagenode monoclonal antibody against hGR </strong><br />The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 &mu;l IP reaction solution) using 5 &mu;g of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050). The IP was followed by Western blot analysis as described above </small></p>
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<p>Monoclonal antibody raised in mouse against amino acids 304-428 of the human <strong>GR (glucocorticoid receptor),</strong> using a chimeric protein.</p>',
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	'name' => 'GR Antibody',
	'description' => '<p>Alternative names: <strong>NR3C1</strong>,<strong> GCCR</strong>, <strong>GCR</strong>, <strong>GRL</strong></p>
<p>Monoclonal antibody raised in mouse against amino acids 304-428 of the human <strong>GR (glucocorticoid receptor)</strong>, using a chimeric protein.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP assays were performed using HeLa cells, the Diagenode monoclonal antibody directed against GR (Cat. No. MAb-010-050) and optimized PCR primer sets for qPCR. The cells were treated either with ethanol (EtOH, used as a negative control) or triamcinolone acetonide (TA) for 4 hours prior to cell harvesting. ChIP was performed using sheared chromatin from 3 million cells and 5 &mu;g of antibody. QPCR was performed with primers for the human metallothionein promoter (hMTIIA) and for exon 2 of the human myoglobin gene (hmyo ex2), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the human metallothionein IIA promoter by GR. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2A.png" alt="GR Antibody ChIP-seq Grade" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP was performed on sheared chromatin from 3.5 million HeLaB2 cells using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). The cells were treated with the synthetic GR ligand triamcinolone acetonide (TA) for 4 hours prior to harvesting. The IP&rsquo;d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence of chromosome 16 (figure 2A) as well as the MT2A positive control gene (figure 2B). The position of the PCR amplicon is also indicated. Figure 2C, D and E show the results for the known GR target genes PER1 on chromosome 17 and FKBP5 and TNFAIP3 on chromosome 6. </small></p>
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<p></p>
<div class="row">
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig3.png" alt="GR Antibody ELISA Validation" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Sandwich ELISA </strong><br />The specificity of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050) was assessed by sandwich ELISA. Figure 3A: schematic representation of the sandwich ELISA with the monoclonal antibody against hGR (clone #:m2F8). Figure 3B: ELISA results using the monoclonal antibody against hGR at a concentration of 0.5 &mu;g/ml. The figure shows an ELISA signal which is proportionally increasing with increasing amounts of recombinant hGR. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig4.png" alt="GR Antibody validated in Western Blot" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode monoclonal antibody against hGR </strong><br />Figure 4A. Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10e6 Raji or Molt cells were analysed by Western blot using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). Figure 4B. Western blot analysis of extracts from 300,000 HeLa cells with the Diagenode monoclonal antibody against hGR (concentration 1 &mu;g/ml). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig6.png" alt="GR Antibody validated for Immunohistochemistry" /></p>
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<p><small><strong> Figure 5. Immunohistochemistry and immunofluorescence using the Diagenode monoclonal antibody against hGR </strong><br />1. Immunoreactivity of the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050) in rat CA1 neurons of hippocampus. The antibody was used at a concentration of 2.5 &mu;g/ml. <br />2. COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary antibody and peroxidase-labeled avidin. The antibody was used at a concentration of 2.5 &mu;g/ml. </small></p>
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<p><small><strong> Figure 6. Immunoprecipitation using the Diagenode monoclonal antibody against hGR </strong><br />The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 &mu;l IP reaction solution) using 5 &mu;g of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050). The IP was followed by Western blot analysis as described above </small></p>
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	'authors' => 'Rao NA, McCalman MT, Moulos P, Francoijs KJ, Chatziioannou A, Kolisis FN, Alexis MN, Mitsiou DJ, Stunnenberg HG',
	'description' => 'Glucocorticoid receptor (GR) exerts anti-inflammatory action in part by antagonizing proinflammatory transcription factors such as the nuclear factor kappa-b (NFKB). Here, we assess the crosstalk of activated GR and RELA (p65, major NFKB component) by global identification of their binding sites and target genes. We show that coactivation of GR and p65 alters the repertoire of regulated genes and results in their association with novel sites in a mutually dependent manner. These novel sites predominantly cluster with p65 target genes that are antagonized by activated GR and vice versa. Our data show that coactivation of GR and NFKB alters signaling pathways that are regulated by each factor separately and provide insight into the networks underlying the GR and NFKB crosstalk.',
	'date' => '2011-09-01',
	'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/21750107',
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<td>Fig 1, 2</td>
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<td>ELISA</td>
<td>0.5 &mu;g/ml</td>
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<tr>
<td>Western Blotting</td>
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<td>Gel Supershift</td>
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<td>Immunochemistry</td>
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<p>Monoclonal antibody raised in mouse against amino acids 304-428 of the human <strong>GR (glucocorticoid receptor),</strong> using a chimeric protein.</p>',
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<div class="small-4 columns">
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<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP assays were performed using HeLa cells, the Diagenode monoclonal antibody directed against GR (Cat. No. MAb-010-050) and optimized PCR primer sets for qPCR. The cells were treated either with ethanol (EtOH, used as a negative control) or triamcinolone acetonide (TA) for 4 hours prior to cell harvesting. ChIP was performed using sheared chromatin from 3 million cells and 5 &mu;g of antibody. QPCR was performed with primers for the human metallothionein promoter (hMTIIA) and for exon 2 of the human myoglobin gene (hmyo ex2), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the human metallothionein IIA promoter by GR. </small></p>
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<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2A.png" alt="GR Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2B.png" alt="GR Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2C.png" alt="GR Antibody for ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2D.png" alt="GR Antibody validated in ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2E.png" alt="GR Antibody ChIP-seq Grade" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP was performed on sheared chromatin from 3.5 million HeLaB2 cells using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). The cells were treated with the synthetic GR ligand triamcinolone acetonide (TA) for 4 hours prior to harvesting. The IP&rsquo;d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence of chromosome 16 (figure 2A) as well as the MT2A positive control gene (figure 2B). The position of the PCR amplicon is also indicated. Figure 2C, D and E show the results for the known GR target genes PER1 on chromosome 17 and FKBP5 and TNFAIP3 on chromosome 6. </small></p>
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<div class="small-6 columns">
<p><small><strong> Figure 3. Sandwich ELISA </strong><br />The specificity of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050) was assessed by sandwich ELISA. Figure 3A: schematic representation of the sandwich ELISA with the monoclonal antibody against hGR (clone #:m2F8). Figure 3B: ELISA results using the monoclonal antibody against hGR at a concentration of 0.5 &mu;g/ml. The figure shows an ELISA signal which is proportionally increasing with increasing amounts of recombinant hGR. </small></p>
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<div class="small-4 columns">
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode monoclonal antibody against hGR </strong><br />Figure 4A. Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10e6 Raji or Molt cells were analysed by Western blot using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). Figure 4B. Western blot analysis of extracts from 300,000 HeLa cells with the Diagenode monoclonal antibody against hGR (concentration 1 &mu;g/ml). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig6.png" alt="GR Antibody validated for Immunohistochemistry" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 5. Immunohistochemistry and immunofluorescence using the Diagenode monoclonal antibody against hGR </strong><br />1. Immunoreactivity of the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050) in rat CA1 neurons of hippocampus. The antibody was used at a concentration of 2.5 &mu;g/ml. <br />2. COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary antibody and peroxidase-labeled avidin. The antibody was used at a concentration of 2.5 &mu;g/ml. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig7.png" alt="GR Antibody validated in Immunoprecipitation" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 6. Immunoprecipitation using the Diagenode monoclonal antibody against hGR </strong><br />The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 &mu;l IP reaction solution) using 5 &mu;g of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050). The IP was followed by Western blot analysis as described above </small></p>
</div>
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		'meta_title' => 'GR Antibody  - ChIP-seq Grade (C15200010) | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'GR (Glucocorticoid receptor) Monoclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB, IP, and ELISA. Batch-specific data available on the website. Alternative names: NR3C1, GCCR, GCR, GRL. Sample size available.',
		'modified' => '2022-01-05 14:49:33',
		'created' => '2017-05-17 14:56:35',
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		'name' => 'GR monoclonal antibody',
		'description' => 'Monoclonal antibody raised in mouse against amino acids 304-428 of the human GR (glucocorticoid receptor), using a chimeric protein.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'NR-010',
		'concentration' => '1.0 &micro;g/&micro;l',
		'reactivity' => 'Human, rat',
		'type' => 'Monoclonal',
		'purity' => 'Ammonium sulphate purified',
		'classification' => 'Classic',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq</td>
<td>5 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>0.5 &mu;g/ml</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1 &mu;g/ml</td>
<td>Fig 4</td>
</tr>
<tr>
<td>Gel Supershift</td>
<td>5 &mu;g/ml</td>
<td></td>
</tr>
<tr>
<td>Immunochemistry</td>
<td>2.5 &mu;g/ml</td>
<td>Fig 5, 6</td>
</tr>
<tr>
<td>Flow cytometry</td>
<td>0.5 &mu;g/ml</td>
<td></td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>5 &mu;g/IP</td>
<td>Fig 7</td>
</tr>
</tbody>
</table>
<p></p>',
		'storage_conditions' => '',
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		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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			'name' => 'GR Antibody',
			'description' => '<p>Alternative names: <strong>NR3C1</strong>,<strong> GCCR</strong>, <strong>GCR</strong>, <strong>GRL</strong></p>
<p>Monoclonal antibody raised in mouse against amino acids 304-428 of the human <strong>GR (glucocorticoid receptor)</strong>, using a chimeric protein.</p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig1.png" alt="GR Antibody ChIP Grade" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP assays were performed using HeLa cells, the Diagenode monoclonal antibody directed against GR (Cat. No. MAb-010-050) and optimized PCR primer sets for qPCR. The cells were treated either with ethanol (EtOH, used as a negative control) or triamcinolone acetonide (TA) for 4 hours prior to cell harvesting. ChIP was performed using sheared chromatin from 3 million cells and 5 &mu;g of antibody. QPCR was performed with primers for the human metallothionein promoter (hMTIIA) and for exon 2 of the human myoglobin gene (hmyo ex2), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the human metallothionein IIA promoter by GR. </small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2A.png" alt="GR Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2B.png" alt="GR Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2C.png" alt="GR Antibody for ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2D.png" alt="GR Antibody validated in ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2E.png" alt="GR Antibody ChIP-seq Grade" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP was performed on sheared chromatin from 3.5 million HeLaB2 cells using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). The cells were treated with the synthetic GR ligand triamcinolone acetonide (TA) for 4 hours prior to harvesting. The IP&rsquo;d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence of chromosome 16 (figure 2A) as well as the MT2A positive control gene (figure 2B). The position of the PCR amplicon is also indicated. Figure 2C, D and E show the results for the known GR target genes PER1 on chromosome 17 and FKBP5 and TNFAIP3 on chromosome 6. </small></p>
</div>
</div>
<p></p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig3.png" alt="GR Antibody ELISA Validation" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Sandwich ELISA </strong><br />The specificity of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050) was assessed by sandwich ELISA. Figure 3A: schematic representation of the sandwich ELISA with the monoclonal antibody against hGR (clone #:m2F8). Figure 3B: ELISA results using the monoclonal antibody against hGR at a concentration of 0.5 &mu;g/ml. The figure shows an ELISA signal which is proportionally increasing with increasing amounts of recombinant hGR. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig4.png" alt="GR Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode monoclonal antibody against hGR </strong><br />Figure 4A. Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10e6 Raji or Molt cells were analysed by Western blot using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). Figure 4B. Western blot analysis of extracts from 300,000 HeLa cells with the Diagenode monoclonal antibody against hGR (concentration 1 &mu;g/ml). </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig5.png" alt="GR Antibody validated in Immunohistochemistry" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig6.png" alt="GR Antibody validated for Immunohistochemistry" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Immunohistochemistry and immunofluorescence using the Diagenode monoclonal antibody against hGR </strong><br />1. Immunoreactivity of the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050) in rat CA1 neurons of hippocampus. The antibody was used at a concentration of 2.5 &mu;g/ml. <br />2. COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary antibody and peroxidase-labeled avidin. The antibody was used at a concentration of 2.5 &mu;g/ml. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig7.png" alt="GR Antibody validated in Immunoprecipitation" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 6. Immunoprecipitation using the Diagenode monoclonal antibody against hGR </strong><br />The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 &mu;l IP reaction solution) using 5 &mu;g of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050). The IP was followed by Western blot analysis as described above </small></p>
</div>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p></p>
<p></p>
<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)',
			'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
			'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
			'modified' => '2019-07-03 10:57:22',
			'created' => '2015-02-16 02:24:01',
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			'id' => '103',
			'position' => '0',
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			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
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			'slug' => 'all-antibodies',
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			'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
			'modified' => '2019-07-03 10:55:44',
			'created' => '2015-11-02 14:49:22',
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			'id' => '102',
			'position' => '1',
			'parent_id' => '4',
			'name' => 'Sample size antibodies',
			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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			'slug' => 'sample-size-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
			'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
			'meta_title' => 'Sample-size Antibodies | Diagenode',
			'modified' => '2019-07-03 10:57:05',
			'created' => '2015-10-27 12:13:34',
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			'id' => '30',
			'position' => '50',
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			'name' => 'Transcription',
			'description' => '<p><span style="font-weight: 400;">The list of Diagenode&rsquo;s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. &nbsp;Check the list below to see our targets.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
</ul>',
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			'slug' => 'transcription-factor',
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			'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies',
			'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie',
			'meta_title' => 'Transcription factor Antibodies | Diagenode',
			'modified' => '2020-07-06 12:59:19',
			'created' => '2015-03-12 10:20:08',
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			'id' => '11',
			'name' => 'Antibodies you can trust',
			'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
			'image_id' => null,
			'type' => 'Poster',
			'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
			'slug' => 'antibodies-you-can-trust-poster',
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			'modified' => '2015-10-01 20:18:31',
			'created' => '2015-07-03 16:05:15',
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			)
		),
		(int) 1 => array(
			'id' => '38',
			'name' => 'Epigenetic Antibodies Brochure',
			'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
			'image_id' => null,
			'type' => 'Brochure',
			'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
			'slug' => 'epigenetic-antibodies-brochure',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2016-06-15 11:24:06',
			'created' => '2015-07-03 16:05:27',
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		(int) 2 => array(
			'id' => '440',
			'name' => 'Datasheet hGR C15200010',
			'description' => '<p>Datasheet description</p>',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/antibodies/Datasheet_hGR_C15200010.pdf',
			'slug' => 'datasheet-hgr-C15200010',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2015-11-20 17:16:18',
			'created' => '2015-07-07 11:47:44',
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			'id' => '1783',
			'name' => 'product/antibodies/chipseq-grade-ab-icon.png',
			'alt' => 'ChIP-seq Grade',
			'modified' => '2020-11-27 07:04:40',
			'created' => '2018-03-15 15:54:09',
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		(int) 0 => array(
			'id' => '4253',
			'name' => 'Coordinated glucocorticoid receptor and MAFB action inducestolerogenesis and epigenome remodeling in dendritic cells',
			'authors' => 'Morante-Palacios Octavio et al.',
			'description' => '<p>Abstract Glucocorticoids (GCs) exert potent anti-inflammatory effects in immune cells through the glucocorticoid receptor (GR). Dendritic cells (DCs), central actors for coordinating immune responses, acquire tolerogenic properties in response to GCs. Tolerogenic DCs (tolDCs) have emerged as a potential treatment for various inflammatory diseases. To date, the underlying cell type-specific regulatory mechanisms orchestrating GC-mediated acquisition of immunosuppressive properties remain poorly understood. In this study, we investigated the transcriptomic and epigenomic remodeling associated with differentiation to DCs in the presence of GCs. Our analysis demonstrates a major role of MAFB in this process, in synergy with GR. GR and MAFB both interact with methylcytosine dioxygenase TET2 and bind to genomic loci that undergo specific demethylation in tolDCs. We also show that the role of MAFB is more extensive, binding to thousands of genomic loci in tolDCs. Finally, MAFB knockdown erases the tolerogenic properties of tolDCs and reverts the specific DNA demethylation and gene upregulation. The preeminent role of MAFB is also demonstrated in vivo for myeloid cells from synovium in rheumatoid arthritis following GC treatment. Our results imply that, once directly activated by GR, MAFB plays a critical role in orchestrating the epigenomic and transcriptomic remodeling that define the tolerogenic phenotype.</p>',
			'date' => '2022-01-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34893889',
			'doi' => '10.1093/nar/gkab1182',
			'modified' => '2022-05-20 09:44:29',
			'created' => '2022-05-19 10:41:50',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '3208',
			'name' => 'The Role of S-Palmitoylation of the Human Glucocorticoid Receptor (hGR) in Mediating the Nongenomic Glucocorticoid Actions',
			'authors' => 'Nicolaides N. et al.',
			'description' => '<p>Background: Many rapid nongenomic glucocorticoid actions are mediated by membrane-bound glucocorticoid receptors (GRs). S-palmitoylation is a lipid post-translational modification that mediates the membrane localization of some steroid receptors. A highly homologous amino acid sequence (663YLCM KTLLL671) is present in the ligand-binding domain of hGR&alpha;, suggesting that hGR&alpha; might also undergo S-palmitoylation.</p>
<p>Aim: To investigate the role of the motif 663YLCMKTLLL671 in membrane localization of the hGR&alpha; and in mediating rapid nongenomic glucocorticoid signaling.</p>
<p>Methods and Results: We showed that the mutant receptors hGR&alpha;Y663A, hGR&alpha;C665A and hGR&alpha;LL670/671AA, and the addition of the palmitoylation inhibitor 2-bromopalmitate did not prevent membrane localization of hGR&alpha; and co-localization with caveolin-1, and did not influence the biphasic activation of mitogen-activated protein kinase (MAPK) signaling pathway in the early time points. Finally, the hGR&alpha; was not shown to undergo S-palmitoylation.</p>
<p>Conclusions: The motif 663YLCMKTLLL671 does not play a role in membrane localization of hGR&alpha; and does not mediate the nongenomic glucocorticoid actions.</p>',
			'date' => '2017-04-15',
			'pmid' => 'http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/199',
			'doi' => '',
			'modified' => '2017-07-07 16:21:23',
			'created' => '2017-07-07 16:21:23',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '2923',
			'name' => 'Three Novel Heterozygous Point Mutations of NR3C1 causing Glucocorticoid Resistance',
			'authors' => 'Vitellius G et al.',
			'description' => '<p>Generalized glucocorticoid resistance is associated with glucocorticoid receptor (GR, NR3C1) mutations. Three novel heterozygous missense NR3C1 mutations (R477S, Y478C and L672P) were identified in patients presenting with adrenal incidentalomas, glucocorticoid excess without Cushing syndrome. Dexamethasone (DXM) binding studies demonstrated that the affinity of GR<sub>R477S</sub> and GR<sub>Y478C</sub> mutants, located in the DNA-binding domain of GR, was similar to wild-type GR (Kd = 2-3 nM). In contrast, GR<sub>L672P</sub> mutant, located in the ligand-binding domain of GR, was unable to bind glucocorticoids and was more sensitive to protein degradation. GR subcellular distribution revealed a marked decrease in DXM-induced nuclear translocation of GR<sub>R477S</sub> and GR<sub>Y478C</sub> mutants, whereas GR<sub>L672P</sub> remained exclusively cytoplasmic. Chromatin immunoprecipitation demonstrated impaired recruitment of DBD mutants onto the regulatory sequence of FKBP5. Transactivation assays disclosed the lack of transcriptional activity of GR<sub>R477S</sub> and GR<sub>L672P</sub> while GR<sub>Y478C</sub> had a reduced transactivation capacity. Three-D modeling indicated that R477S lost two essential hydrogen bonds with DNA, Y478C resulted in altered interaction with surrounding amino-acids, destabilizing DBD, while L672P altered the H8 helix folding, leading to unstructured LBD. This study identifies novel NR3C1 mutations with their molecular consequences on altered GR signaling and suggests that genetic screening of NR3C1 should be conducted in patients with subclinical hypercorticism.</p>',
			'date' => '2016-04-27',
			'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27120390',
			'doi' => '10.1002/humu.23008',
			'modified' => '2016-05-13 17:32:26',
			'created' => '2016-05-13 17:32:26',
			'ProductsPublication' => array(
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			)
		),
		(int) 3 => array(
			'id' => '2967',
			'name' => 'Functional characterization of the hGRαT556I causing Chrousos syndrome',
			'authors' => 'Nicolaides NC et al.',
			'description' => '<h4>BACKGROUND:</h4>
<p><abstracttext label="BACKGROUND" nlmcategory="BACKGROUND">Chrousos syndrome is a rare pathologic condition characterized by generalized, partial resistance of target tissues to glucocorticoids and caused by inactivating mutations of the human glucocorticoid receptor (hGR) gene. A novel case of Chrousos syndrome has been reported in a patient with adrenal incidentaloma, who harboured a heterozygous point mutation in the hGR gene, which resulted in threonine (T) to isoleucine (I) substitution at amino acid position 556 in the ligand-binding domain of the receptor.</abstracttext></p>
<h4>OBJECTIVE:</h4>
<p><abstracttext label="OBJECTIVE" nlmcategory="OBJECTIVE">To delineate the molecular mechanisms through which the mutant receptor hGR&alpha;T556I causes Chrousos syndrome.</abstracttext></p>
<h4>DESIGN AND RESULTS:</h4>
<p><abstracttext label="DESIGN AND RESULTS" nlmcategory="RESULTS">Compared with the wild-type receptor, the mutant receptor hGR&alpha;T556I demonstrated 50% reduction in its ability to transactivate glucocorticoid-responsive genes and in the affinity for the ligand, 30% increase in the ability to transrepress the nuclear factor-&kappa;B-target genes and a 3,4-fold delay in the cytoplasmic-to-nuclear translocation. The mutant receptor hGR&alpha;T556I did not exert a dominant negative effect upon the hGR&alpha;-mediated transcriptional activity; it preserved its ability to bind to DNA and interacted with the glucocorticoid receptor-interacting protein 1 coactivator mostly through its activation function-1 domain. Structural biology studies revealed that the T556I mutation caused disruption of the hydrogen bond formed by the T556 with the =O group of P637 backbone, which resulted in a significant relocation of the P637-bearing loop. This conformational alteration affected the local 3D arrangement of the receptor and hence the electrostatic surface of the region.</abstracttext></p>
<h4>CONCLUSIONS:</h4>
<p><abstracttext label="CONCLUSIONS" nlmcategory="CONCLUSIONS">The hGR&alpha;T556I causes Chrousos syndrome by impairing multiple steps of the glucocorticoid signal transduction pathway.</abstracttext></p>',
			'date' => '2016-01-01',
			'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26541474',
			'doi' => ' 10.1111/eci.12563',
			'modified' => '2016-06-29 09:41:42',
			'created' => '2016-06-29 09:41:42',
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				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '1776',
			'name' => 'Perinatal exposure to low-dose bisphenol A affects the neuroendocrine stress response in rats.',
			'authors' => 'Panagiotidou E, Zerva S, Mitsiou DJ, Alexis MN, Kitraki E',
			'description' => 'Bisphenol A (BPA) is an estrogen-mimicking endocrine disruptor. Early-life exposures to low doses of BPA exert long-lasting effects on animals' reproductive and brain physiology. However, little is known about the effects of BPA on the stress-response system. Given the interaction of sex and stress hormones, we examined the effect of a low perinatal BPA exposure on the function of the hypothalamic-pituitary-adrenal (HPA) axis at rest and upon application of acute stress. Throughout pregnancy and lactation rats received daily 40 μg BPA/kg body weight orally via cornflakes. We studied the effect of this low but chronic exposure to BPA in the male and female offspring at puberty. BPA exposure led to abnormal adrenal histology including reduced zona reticularis especially in male offspring, hyperplasia of zona fasciculata in both sexes, and increased adrenal weight in female offspring. BPA-treated females had increased basal corticosterone and reduced hypothalamic glucocorticoid receptors (GR) levels. Stressed BPA-exposed females exhibited anxiety-like behavioral coping, a less rigorous corticosterone response, and did not downregulate GR in the hypothalamus, compared with control females. BPA-exposed males exhibited a heightened corticosterone stress response compared with females; they also displayed increased pro-opiomelanocortin mRNA levels and retained the prestress levels of pituitary corticotropin-releasing hormone-receptor 1, compared with control males. We found that perinatal chronic exposure to a low dose of BPA perturbs the basal and stress-induced activity of the HPA axis in a sexually dimorphic manner at adolescence. Exposure to BPA might contribute to increased susceptibility to stress-related disorders in later life.',
			'date' => '2014-03-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/24323913',
			'doi' => '',
			'modified' => '2015-07-24 15:39:01',
			'created' => '2015-07-24 15:39:01',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '1557',
			'name' => 'Pattern of heat shock factor and heat shock protein expression in lymphocytes of bipolar patients: Increased HSP70-glucocorticoid receptor heterocomplex.',
			'authors' => 'Bei ES, Salpeas V, Alevizos B, Anagnostara C, Pappa D, Moutsatsou P',
			'description' => 'Bipolar disorder (BD), a stress-related disease, is characterized by altered glucocorticoid receptor (GR) signalling. Stress response includes activation of heat shock factor (HSF) and subsequent heat shock protein (HSP) synthesis which regulate GR folding and function. The objective of this study was to investigate the possible role of HSFs, HSPs and their interaction with GR in BD. We applied immunoprecipitation, SDS-PAGE/Western blot analysis and electrophoretic mobility shift assay (EMSA) in lymphocytes (whole cell or nuclear extracts) from BD patients and healthy subjects and determined the HSPs (HSP90 and HSP70), the heterocomplexes HSP90-GR and HSP70-GR, the HSFs (HSF1 and HSF4) as well as the HSF-DNA binding. The HSP70-GR heterocomplex was elevated (p < 0.05) in BD patients vs healthy subjects, and nuclear HSP70 was reduced (p ≤ 0.01) in bipolar manic patients. Protein levels of HSF1, HSF4, HSP90, HSP90-GR heterocomplex, and HSF-DNA binding remained unaltered in BD patients vs healthy subjects. The corresponding effect sizes (ES) indicated a large ES for HSP70-GR, HSP70, HSF-DNA binding and HSF4, and a medium ES for HSP90, HSF1 and HSP90-GR between healthy subjects and bipolar patients. Significant correlations among HSFs, HSPs, GR and HSP70-GR heterocomplex were observed in healthy subjects, which were abrogated in bipolar patients. The higher interaction between GR and HSP70 and the disturbances in the relations among heat shock response parameters and GR as observed in our BD patients may provide novel insights into the contribution of these factors in BD aetiopathogenesis.',
			'date' => '2013-11-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/23938235',
			'doi' => '',
			'modified' => '2015-07-24 15:39:00',
			'created' => '2015-07-24 15:39:00',
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		(int) 6 => array(
			'id' => '253',
			'name' => 'Coactivation of GR and NFKB alters the repertoire of their binding sites and target genes.',
			'authors' => 'Rao NA, McCalman MT, Moulos P, Francoijs KJ, Chatziioannou A, Kolisis FN, Alexis MN, Mitsiou DJ, Stunnenberg HG',
			'description' => 'Glucocorticoid receptor (GR) exerts anti-inflammatory action in part by antagonizing proinflammatory transcription factors such as the nuclear factor kappa-b (NFKB). Here, we assess the crosstalk of activated GR and RELA (p65, major NFKB component) by global identification of their binding sites and target genes. We show that coactivation of GR and p65 alters the repertoire of regulated genes and results in their association with novel sites in a mutually dependent manner. These novel sites predominantly cluster with p65 target genes that are antagonized by activated GR and vice versa. Our data show that coactivation of GR and NFKB alters signaling pathways that are regulated by each factor separately and provide insight into the networks underlying the GR and NFKB crosstalk.',
			'date' => '2011-09-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/21750107',
			'doi' => '',
			'modified' => '2015-07-24 15:38:57',
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			'id' => '566',
			'name' => 'GR antibody SDS GB en',
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			'countries' => 'GB',
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			'name' => 'GR antibody SDS US en',
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			'url' => 'files/SDS/GR/SDS-C15200010-GR_Antibody-US-en-GHS_2_0.pdf',
			'countries' => 'US',
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			'url' => 'files/SDS/GR/SDS-C15200010-GR_Antibody-DE-de-GHS_2_0.pdf',
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			'url' => 'files/SDS/GR/SDS-C15200010-GR_Antibody-JP-ja-GHS_2_0.pdf',
			'countries' => 'JP',
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP was performed on sheared chromatin from 3.5 million HeLaB2 cells using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). The cells were treated with the synthetic GR ligand triamcinolone acetonide (TA) for 4 hours prior to harvesting. The IP&rsquo;d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence of chromosome 16 (figure 2A) as well as the MT2A positive control gene (figure 2B). The position of the PCR amplicon is also indicated. Figure 2C, D and E show the results for the known GR target genes PER1 on chromosome 17 and FKBP5 and TNFAIP3 on chromosome 6. </small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode monoclonal antibody against hGR </strong><br />Figure 4A. Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10e6 Raji or Molt cells were analysed by Western blot using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). Figure 4B. Western blot analysis of extracts from 300,000 HeLa cells with the Diagenode monoclonal antibody against hGR (concentration 1 &mu;g/ml). </small></p>
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<p><small><strong> Figure 5. Immunohistochemistry and immunofluorescence using the Diagenode monoclonal antibody against hGR </strong><br />1. Immunoreactivity of the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050) in rat CA1 neurons of hippocampus. The antibody was used at a concentration of 2.5 &mu;g/ml. <br />2. COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary antibody and peroxidase-labeled avidin. The antibody was used at a concentration of 2.5 &mu;g/ml. </small></p>
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP assays were performed using HeLa cells, the Diagenode monoclonal antibody directed against GR (Cat. No. MAb-010-050) and optimized PCR primer sets for qPCR. The cells were treated either with ethanol (EtOH, used as a negative control) or triamcinolone acetonide (TA) for 4 hours prior to cell harvesting. ChIP was performed using sheared chromatin from 3 million cells and 5 &mu;g of antibody. QPCR was performed with primers for the human metallothionein promoter (hMTIIA) and for exon 2 of the human myoglobin gene (hmyo ex2), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the human metallothionein IIA promoter by GR. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2A.png" alt="GR Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2B.png" alt="GR Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2C.png" alt="GR Antibody for ChIP-seq assay" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP was performed on sheared chromatin from 3.5 million HeLaB2 cells using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). The cells were treated with the synthetic GR ligand triamcinolone acetonide (TA) for 4 hours prior to harvesting. The IP&rsquo;d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence of chromosome 16 (figure 2A) as well as the MT2A positive control gene (figure 2B). The position of the PCR amplicon is also indicated. Figure 2C, D and E show the results for the known GR target genes PER1 on chromosome 17 and FKBP5 and TNFAIP3 on chromosome 6. </small></p>
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<p><small><strong> Figure 3. Sandwich ELISA </strong><br />The specificity of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050) was assessed by sandwich ELISA. Figure 3A: schematic representation of the sandwich ELISA with the monoclonal antibody against hGR (clone #:m2F8). Figure 3B: ELISA results using the monoclonal antibody against hGR at a concentration of 0.5 &mu;g/ml. The figure shows an ELISA signal which is proportionally increasing with increasing amounts of recombinant hGR. </small></p>
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<div class="small-4 columns">
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig6.png" alt="GR Antibody validated for Immunohistochemistry" /></p>
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<p><small><strong> Figure 5. Immunohistochemistry and immunofluorescence using the Diagenode monoclonal antibody against hGR </strong><br />1. Immunoreactivity of the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050) in rat CA1 neurons of hippocampus. The antibody was used at a concentration of 2.5 &mu;g/ml. <br />2. COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary antibody and peroxidase-labeled avidin. The antibody was used at a concentration of 2.5 &mu;g/ml. </small></p>
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<div class="small-4 columns">
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<p><small><strong> Figure 6. Immunoprecipitation using the Diagenode monoclonal antibody against hGR </strong><br />The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 &mu;l IP reaction solution) using 5 &mu;g of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050). The IP was followed by Western blot analysis as described above </small></p>
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<td>0.5 &mu;g/ml</td>
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<div class="small-4 columns">
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</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2A.png" alt="GR Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2B.png" alt="GR Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2C.png" alt="GR Antibody for ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2D.png" alt="GR Antibody validated in ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2E.png" alt="GR Antibody ChIP-seq Grade" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP was performed on sheared chromatin from 3.5 million HeLaB2 cells using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). The cells were treated with the synthetic GR ligand triamcinolone acetonide (TA) for 4 hours prior to harvesting. The IP&rsquo;d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence of chromosome 16 (figure 2A) as well as the MT2A positive control gene (figure 2B). The position of the PCR amplicon is also indicated. Figure 2C, D and E show the results for the known GR target genes PER1 on chromosome 17 and FKBP5 and TNFAIP3 on chromosome 6. </small></p>
</div>
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<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig3.png" alt="GR Antibody ELISA Validation" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Sandwich ELISA </strong><br />The specificity of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050) was assessed by sandwich ELISA. Figure 3A: schematic representation of the sandwich ELISA with the monoclonal antibody against hGR (clone #:m2F8). Figure 3B: ELISA results using the monoclonal antibody against hGR at a concentration of 0.5 &mu;g/ml. The figure shows an ELISA signal which is proportionally increasing with increasing amounts of recombinant hGR. </small></p>
</div>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig4.png" alt="GR Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode monoclonal antibody against hGR </strong><br />Figure 4A. Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10e6 Raji or Molt cells were analysed by Western blot using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). Figure 4B. Western blot analysis of extracts from 300,000 HeLa cells with the Diagenode monoclonal antibody against hGR (concentration 1 &mu;g/ml). </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig5.png" alt="GR Antibody validated in Immunohistochemistry" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig6.png" alt="GR Antibody validated for Immunohistochemistry" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 5. Immunohistochemistry and immunofluorescence using the Diagenode monoclonal antibody against hGR </strong><br />1. Immunoreactivity of the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050) in rat CA1 neurons of hippocampus. The antibody was used at a concentration of 2.5 &mu;g/ml. <br />2. COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary antibody and peroxidase-labeled avidin. The antibody was used at a concentration of 2.5 &mu;g/ml. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig7.png" alt="GR Antibody validated in Immunoprecipitation" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 6. Immunoprecipitation using the Diagenode monoclonal antibody against hGR </strong><br />The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 &mu;l IP reaction solution) using 5 &mu;g of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050). The IP was followed by Western blot analysis as described above </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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			'description' => '<p><span style="font-weight: 400;">The list of Diagenode&rsquo;s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. &nbsp;Check the list below to see our targets.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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			'name' => 'Coordinated glucocorticoid receptor and MAFB action inducestolerogenesis and epigenome remodeling in dendritic cells',
			'authors' => 'Morante-Palacios Octavio et al.',
			'description' => '<p>Abstract Glucocorticoids (GCs) exert potent anti-inflammatory effects in immune cells through the glucocorticoid receptor (GR). Dendritic cells (DCs), central actors for coordinating immune responses, acquire tolerogenic properties in response to GCs. Tolerogenic DCs (tolDCs) have emerged as a potential treatment for various inflammatory diseases. To date, the underlying cell type-specific regulatory mechanisms orchestrating GC-mediated acquisition of immunosuppressive properties remain poorly understood. In this study, we investigated the transcriptomic and epigenomic remodeling associated with differentiation to DCs in the presence of GCs. Our analysis demonstrates a major role of MAFB in this process, in synergy with GR. GR and MAFB both interact with methylcytosine dioxygenase TET2 and bind to genomic loci that undergo specific demethylation in tolDCs. We also show that the role of MAFB is more extensive, binding to thousands of genomic loci in tolDCs. Finally, MAFB knockdown erases the tolerogenic properties of tolDCs and reverts the specific DNA demethylation and gene upregulation. The preeminent role of MAFB is also demonstrated in vivo for myeloid cells from synovium in rheumatoid arthritis following GC treatment. Our results imply that, once directly activated by GR, MAFB plays a critical role in orchestrating the epigenomic and transcriptomic remodeling that define the tolerogenic phenotype.</p>',
			'date' => '2022-01-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34893889',
			'doi' => '10.1093/nar/gkab1182',
			'modified' => '2022-05-20 09:44:29',
			'created' => '2022-05-19 10:41:50',
			'ProductsPublication' => array(
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		),
		(int) 1 => array(
			'id' => '3208',
			'name' => 'The Role of S-Palmitoylation of the Human Glucocorticoid Receptor (hGR) in Mediating the Nongenomic Glucocorticoid Actions',
			'authors' => 'Nicolaides N. et al.',
			'description' => '<p>Background: Many rapid nongenomic glucocorticoid actions are mediated by membrane-bound glucocorticoid receptors (GRs). S-palmitoylation is a lipid post-translational modification that mediates the membrane localization of some steroid receptors. A highly homologous amino acid sequence (663YLCM KTLLL671) is present in the ligand-binding domain of hGR&alpha;, suggesting that hGR&alpha; might also undergo S-palmitoylation.</p>
<p>Aim: To investigate the role of the motif 663YLCMKTLLL671 in membrane localization of the hGR&alpha; and in mediating rapid nongenomic glucocorticoid signaling.</p>
<p>Methods and Results: We showed that the mutant receptors hGR&alpha;Y663A, hGR&alpha;C665A and hGR&alpha;LL670/671AA, and the addition of the palmitoylation inhibitor 2-bromopalmitate did not prevent membrane localization of hGR&alpha; and co-localization with caveolin-1, and did not influence the biphasic activation of mitogen-activated protein kinase (MAPK) signaling pathway in the early time points. Finally, the hGR&alpha; was not shown to undergo S-palmitoylation.</p>
<p>Conclusions: The motif 663YLCMKTLLL671 does not play a role in membrane localization of hGR&alpha; and does not mediate the nongenomic glucocorticoid actions.</p>',
			'date' => '2017-04-15',
			'pmid' => 'http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/199',
			'doi' => '',
			'modified' => '2017-07-07 16:21:23',
			'created' => '2017-07-07 16:21:23',
			'ProductsPublication' => array(
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		),
		(int) 2 => array(
			'id' => '2923',
			'name' => 'Three Novel Heterozygous Point Mutations of NR3C1 causing Glucocorticoid Resistance',
			'authors' => 'Vitellius G et al.',
			'description' => '<p>Generalized glucocorticoid resistance is associated with glucocorticoid receptor (GR, NR3C1) mutations. Three novel heterozygous missense NR3C1 mutations (R477S, Y478C and L672P) were identified in patients presenting with adrenal incidentalomas, glucocorticoid excess without Cushing syndrome. Dexamethasone (DXM) binding studies demonstrated that the affinity of GR<sub>R477S</sub> and GR<sub>Y478C</sub> mutants, located in the DNA-binding domain of GR, was similar to wild-type GR (Kd = 2-3 nM). In contrast, GR<sub>L672P</sub> mutant, located in the ligand-binding domain of GR, was unable to bind glucocorticoids and was more sensitive to protein degradation. GR subcellular distribution revealed a marked decrease in DXM-induced nuclear translocation of GR<sub>R477S</sub> and GR<sub>Y478C</sub> mutants, whereas GR<sub>L672P</sub> remained exclusively cytoplasmic. Chromatin immunoprecipitation demonstrated impaired recruitment of DBD mutants onto the regulatory sequence of FKBP5. Transactivation assays disclosed the lack of transcriptional activity of GR<sub>R477S</sub> and GR<sub>L672P</sub> while GR<sub>Y478C</sub> had a reduced transactivation capacity. Three-D modeling indicated that R477S lost two essential hydrogen bonds with DNA, Y478C resulted in altered interaction with surrounding amino-acids, destabilizing DBD, while L672P altered the H8 helix folding, leading to unstructured LBD. This study identifies novel NR3C1 mutations with their molecular consequences on altered GR signaling and suggests that genetic screening of NR3C1 should be conducted in patients with subclinical hypercorticism.</p>',
			'date' => '2016-04-27',
			'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27120390',
			'doi' => '10.1002/humu.23008',
			'modified' => '2016-05-13 17:32:26',
			'created' => '2016-05-13 17:32:26',
			'ProductsPublication' => array(
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		),
		(int) 3 => array(
			'id' => '2967',
			'name' => 'Functional characterization of the hGRαT556I causing Chrousos syndrome',
			'authors' => 'Nicolaides NC et al.',
			'description' => '<h4>BACKGROUND:</h4>
<p><abstracttext label="BACKGROUND" nlmcategory="BACKGROUND">Chrousos syndrome is a rare pathologic condition characterized by generalized, partial resistance of target tissues to glucocorticoids and caused by inactivating mutations of the human glucocorticoid receptor (hGR) gene. A novel case of Chrousos syndrome has been reported in a patient with adrenal incidentaloma, who harboured a heterozygous point mutation in the hGR gene, which resulted in threonine (T) to isoleucine (I) substitution at amino acid position 556 in the ligand-binding domain of the receptor.</abstracttext></p>
<h4>OBJECTIVE:</h4>
<p><abstracttext label="OBJECTIVE" nlmcategory="OBJECTIVE">To delineate the molecular mechanisms through which the mutant receptor hGR&alpha;T556I causes Chrousos syndrome.</abstracttext></p>
<h4>DESIGN AND RESULTS:</h4>
<p><abstracttext label="DESIGN AND RESULTS" nlmcategory="RESULTS">Compared with the wild-type receptor, the mutant receptor hGR&alpha;T556I demonstrated 50% reduction in its ability to transactivate glucocorticoid-responsive genes and in the affinity for the ligand, 30% increase in the ability to transrepress the nuclear factor-&kappa;B-target genes and a 3,4-fold delay in the cytoplasmic-to-nuclear translocation. The mutant receptor hGR&alpha;T556I did not exert a dominant negative effect upon the hGR&alpha;-mediated transcriptional activity; it preserved its ability to bind to DNA and interacted with the glucocorticoid receptor-interacting protein 1 coactivator mostly through its activation function-1 domain. Structural biology studies revealed that the T556I mutation caused disruption of the hydrogen bond formed by the T556 with the =O group of P637 backbone, which resulted in a significant relocation of the P637-bearing loop. This conformational alteration affected the local 3D arrangement of the receptor and hence the electrostatic surface of the region.</abstracttext></p>
<h4>CONCLUSIONS:</h4>
<p><abstracttext label="CONCLUSIONS" nlmcategory="CONCLUSIONS">The hGR&alpha;T556I causes Chrousos syndrome by impairing multiple steps of the glucocorticoid signal transduction pathway.</abstracttext></p>',
			'date' => '2016-01-01',
			'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26541474',
			'doi' => ' 10.1111/eci.12563',
			'modified' => '2016-06-29 09:41:42',
			'created' => '2016-06-29 09:41:42',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '1776',
			'name' => 'Perinatal exposure to low-dose bisphenol A affects the neuroendocrine stress response in rats.',
			'authors' => 'Panagiotidou E, Zerva S, Mitsiou DJ, Alexis MN, Kitraki E',
			'description' => 'Bisphenol A (BPA) is an estrogen-mimicking endocrine disruptor. Early-life exposures to low doses of BPA exert long-lasting effects on animals' reproductive and brain physiology. However, little is known about the effects of BPA on the stress-response system. Given the interaction of sex and stress hormones, we examined the effect of a low perinatal BPA exposure on the function of the hypothalamic-pituitary-adrenal (HPA) axis at rest and upon application of acute stress. Throughout pregnancy and lactation rats received daily 40 μg BPA/kg body weight orally via cornflakes. We studied the effect of this low but chronic exposure to BPA in the male and female offspring at puberty. BPA exposure led to abnormal adrenal histology including reduced zona reticularis especially in male offspring, hyperplasia of zona fasciculata in both sexes, and increased adrenal weight in female offspring. BPA-treated females had increased basal corticosterone and reduced hypothalamic glucocorticoid receptors (GR) levels. Stressed BPA-exposed females exhibited anxiety-like behavioral coping, a less rigorous corticosterone response, and did not downregulate GR in the hypothalamus, compared with control females. BPA-exposed males exhibited a heightened corticosterone stress response compared with females; they also displayed increased pro-opiomelanocortin mRNA levels and retained the prestress levels of pituitary corticotropin-releasing hormone-receptor 1, compared with control males. We found that perinatal chronic exposure to a low dose of BPA perturbs the basal and stress-induced activity of the HPA axis in a sexually dimorphic manner at adolescence. Exposure to BPA might contribute to increased susceptibility to stress-related disorders in later life.',
			'date' => '2014-03-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/24323913',
			'doi' => '',
			'modified' => '2015-07-24 15:39:01',
			'created' => '2015-07-24 15:39:01',
			'ProductsPublication' => array(
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		),
		(int) 5 => array(
			'id' => '1557',
			'name' => 'Pattern of heat shock factor and heat shock protein expression in lymphocytes of bipolar patients: Increased HSP70-glucocorticoid receptor heterocomplex.',
			'authors' => 'Bei ES, Salpeas V, Alevizos B, Anagnostara C, Pappa D, Moutsatsou P',
			'description' => 'Bipolar disorder (BD), a stress-related disease, is characterized by altered glucocorticoid receptor (GR) signalling. Stress response includes activation of heat shock factor (HSF) and subsequent heat shock protein (HSP) synthesis which regulate GR folding and function. The objective of this study was to investigate the possible role of HSFs, HSPs and their interaction with GR in BD. We applied immunoprecipitation, SDS-PAGE/Western blot analysis and electrophoretic mobility shift assay (EMSA) in lymphocytes (whole cell or nuclear extracts) from BD patients and healthy subjects and determined the HSPs (HSP90 and HSP70), the heterocomplexes HSP90-GR and HSP70-GR, the HSFs (HSF1 and HSF4) as well as the HSF-DNA binding. The HSP70-GR heterocomplex was elevated (p < 0.05) in BD patients vs healthy subjects, and nuclear HSP70 was reduced (p ≤ 0.01) in bipolar manic patients. Protein levels of HSF1, HSF4, HSP90, HSP90-GR heterocomplex, and HSF-DNA binding remained unaltered in BD patients vs healthy subjects. The corresponding effect sizes (ES) indicated a large ES for HSP70-GR, HSP70, HSF-DNA binding and HSF4, and a medium ES for HSP90, HSF1 and HSP90-GR between healthy subjects and bipolar patients. Significant correlations among HSFs, HSPs, GR and HSP70-GR heterocomplex were observed in healthy subjects, which were abrogated in bipolar patients. The higher interaction between GR and HSP70 and the disturbances in the relations among heat shock response parameters and GR as observed in our BD patients may provide novel insights into the contribution of these factors in BD aetiopathogenesis.',
			'date' => '2013-11-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/23938235',
			'doi' => '',
			'modified' => '2015-07-24 15:39:00',
			'created' => '2015-07-24 15:39:00',
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		(int) 6 => array(
			'id' => '253',
			'name' => 'Coactivation of GR and NFKB alters the repertoire of their binding sites and target genes.',
			'authors' => 'Rao NA, McCalman MT, Moulos P, Francoijs KJ, Chatziioannou A, Kolisis FN, Alexis MN, Mitsiou DJ, Stunnenberg HG',
			'description' => 'Glucocorticoid receptor (GR) exerts anti-inflammatory action in part by antagonizing proinflammatory transcription factors such as the nuclear factor kappa-b (NFKB). Here, we assess the crosstalk of activated GR and RELA (p65, major NFKB component) by global identification of their binding sites and target genes. We show that coactivation of GR and p65 alters the repertoire of regulated genes and results in their association with novel sites in a mutually dependent manner. These novel sites predominantly cluster with p65 target genes that are antagonized by activated GR and vice versa. Our data show that coactivation of GR and NFKB alters signaling pathways that are regulated by each factor separately and provide insight into the networks underlying the GR and NFKB crosstalk.',
			'date' => '2011-09-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/21750107',
			'doi' => '',
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		'id' => '1970',
		'antibody_id' => '326',
		'name' => 'GR Antibody',
		'description' => '<p>Alternative names: <strong>NR3C1</strong>,<strong> GCCR</strong>, <strong>GCR</strong>, <strong>GRL</strong></p>
<p>Monoclonal antibody raised in mouse against amino acids 304-428 of the human <strong>GR (glucocorticoid receptor)</strong>, using a chimeric protein.</p>',
		'label1' => 'Validation Data',
		'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig1.png" alt="GR Antibody ChIP Grade" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP assays were performed using HeLa cells, the Diagenode monoclonal antibody directed against GR (Cat. No. MAb-010-050) and optimized PCR primer sets for qPCR. The cells were treated either with ethanol (EtOH, used as a negative control) or triamcinolone acetonide (TA) for 4 hours prior to cell harvesting. ChIP was performed using sheared chromatin from 3 million cells and 5 &mu;g of antibody. QPCR was performed with primers for the human metallothionein promoter (hMTIIA) and for exon 2 of the human myoglobin gene (hmyo ex2), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the human metallothionein IIA promoter by GR. </small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2A.png" alt="GR Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2B.png" alt="GR Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2C.png" alt="GR Antibody for ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2D.png" alt="GR Antibody validated in ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2E.png" alt="GR Antibody ChIP-seq Grade" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP was performed on sheared chromatin from 3.5 million HeLaB2 cells using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). The cells were treated with the synthetic GR ligand triamcinolone acetonide (TA) for 4 hours prior to harvesting. The IP&rsquo;d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence of chromosome 16 (figure 2A) as well as the MT2A positive control gene (figure 2B). The position of the PCR amplicon is also indicated. Figure 2C, D and E show the results for the known GR target genes PER1 on chromosome 17 and FKBP5 and TNFAIP3 on chromosome 6. </small></p>
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</div>
<p></p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig3.png" alt="GR Antibody ELISA Validation" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Sandwich ELISA </strong><br />The specificity of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050) was assessed by sandwich ELISA. Figure 3A: schematic representation of the sandwich ELISA with the monoclonal antibody against hGR (clone #:m2F8). Figure 3B: ELISA results using the monoclonal antibody against hGR at a concentration of 0.5 &mu;g/ml. The figure shows an ELISA signal which is proportionally increasing with increasing amounts of recombinant hGR. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig4.png" alt="GR Antibody validated in Western Blot" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode monoclonal antibody against hGR </strong><br />Figure 4A. Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10e6 Raji or Molt cells were analysed by Western blot using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). Figure 4B. Western blot analysis of extracts from 300,000 HeLa cells with the Diagenode monoclonal antibody against hGR (concentration 1 &mu;g/ml). </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig5.png" alt="GR Antibody validated in Immunohistochemistry" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig6.png" alt="GR Antibody validated for Immunohistochemistry" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Immunohistochemistry and immunofluorescence using the Diagenode monoclonal antibody against hGR </strong><br />1. Immunoreactivity of the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050) in rat CA1 neurons of hippocampus. The antibody was used at a concentration of 2.5 &mu;g/ml. <br />2. COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary antibody and peroxidase-labeled avidin. The antibody was used at a concentration of 2.5 &mu;g/ml. </small></p>
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</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig7.png" alt="GR Antibody validated in Immunoprecipitation" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 6. Immunoprecipitation using the Diagenode monoclonal antibody against hGR </strong><br />The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 &mu;l IP reaction solution) using 5 &mu;g of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050). The IP was followed by Western blot analysis as described above </small></p>
</div>
</div>',
		'label2' => 'Target Description',
		'info2' => '<p>Monoclonal antibody raised in mouse against amino acids 304-428 of the human GR (glucocorticoid receptor), using a chimeric protein.</p>',
		'label3' => '',
		'info3' => '',
		'format' => '50 µg',
		'catalog_number' => 'C15200010',
		'old_catalog_number' => 'MAb-010-050',
		'sf_code' => 'C15200010-D001-000581',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '380',
		'price_USD' => '380',
		'price_GBP' => '340',
		'price_JPY' => '59525',
		'price_CNY' => '',
		'price_AUD' => '950',
		'country' => 'ALL',
		'except_countries' => 'None',
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		'meta_title' => 'GR Antibody  - ChIP-seq Grade (C15200010) | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'GR (Glucocorticoid receptor) Monoclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB, Flow Cyt, IP and ELISA. Batch-specific data available on the website. Alternative names: NR3C1, GCCR, GCR, GRL. Sample size avaialble.',
		'modified' => '2024-11-19 16:56:51',
		'created' => '2015-06-29 14:08:20'
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<p>Monoclonal antibody raised in mouse against amino acids 304-428 of the human <strong>GR (glucocorticoid receptor),</strong> using a chimeric protein.</p>',
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	'format' => '10 µg',
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	'type' => 'FRE',
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	'meta_title' => 'GR Antibody  - ChIP-seq Grade (C15200010) | Diagenode',
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	'meta_description' => 'GR (Glucocorticoid receptor) Monoclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB, IP, and ELISA. Batch-specific data available on the website. Alternative names: NR3C1, GCCR, GCR, GRL. Sample size available.',
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	'id' => '1970',
	'antibody_id' => '326',
	'name' => 'GR Antibody',
	'description' => '<p>Alternative names: <strong>NR3C1</strong>,<strong> GCCR</strong>, <strong>GCR</strong>, <strong>GRL</strong></p>
<p>Monoclonal antibody raised in mouse against amino acids 304-428 of the human <strong>GR (glucocorticoid receptor)</strong>, using a chimeric protein.</p>',
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	'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig1.png" alt="GR Antibody ChIP Grade" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP assays were performed using HeLa cells, the Diagenode monoclonal antibody directed against GR (Cat. No. MAb-010-050) and optimized PCR primer sets for qPCR. The cells were treated either with ethanol (EtOH, used as a negative control) or triamcinolone acetonide (TA) for 4 hours prior to cell harvesting. ChIP was performed using sheared chromatin from 3 million cells and 5 &mu;g of antibody. QPCR was performed with primers for the human metallothionein promoter (hMTIIA) and for exon 2 of the human myoglobin gene (hmyo ex2), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the human metallothionein IIA promoter by GR. </small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2A.png" alt="GR Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2B.png" alt="GR Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2C.png" alt="GR Antibody for ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2D.png" alt="GR Antibody validated in ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig2E.png" alt="GR Antibody ChIP-seq Grade" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against hGR </strong><br />ChIP was performed on sheared chromatin from 3.5 million HeLaB2 cells using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). The cells were treated with the synthetic GR ligand triamcinolone acetonide (TA) for 4 hours prior to harvesting. The IP&rsquo;d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence of chromosome 16 (figure 2A) as well as the MT2A positive control gene (figure 2B). The position of the PCR amplicon is also indicated. Figure 2C, D and E show the results for the known GR target genes PER1 on chromosome 17 and FKBP5 and TNFAIP3 on chromosome 6. </small></p>
</div>
</div>
<p></p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig3.png" alt="GR Antibody ELISA Validation" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Sandwich ELISA </strong><br />The specificity of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050) was assessed by sandwich ELISA. Figure 3A: schematic representation of the sandwich ELISA with the monoclonal antibody against hGR (clone #:m2F8). Figure 3B: ELISA results using the monoclonal antibody against hGR at a concentration of 0.5 &mu;g/ml. The figure shows an ELISA signal which is proportionally increasing with increasing amounts of recombinant hGR. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig4.png" alt="GR Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode monoclonal antibody against hGR </strong><br />Figure 4A. Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10e6 Raji or Molt cells were analysed by Western blot using the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050). Figure 4B. Western blot analysis of extracts from 300,000 HeLa cells with the Diagenode monoclonal antibody against hGR (concentration 1 &mu;g/ml). </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig5.png" alt="GR Antibody validated in Immunohistochemistry" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig6.png" alt="GR Antibody validated for Immunohistochemistry" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Immunohistochemistry and immunofluorescence using the Diagenode monoclonal antibody against hGR </strong><br />1. Immunoreactivity of the Diagenode monoclonal antibody against hGR (Cat. No. MAb-010-050) in rat CA1 neurons of hippocampus. The antibody was used at a concentration of 2.5 &mu;g/ml. <br />2. COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary antibody and peroxidase-labeled avidin. The antibody was used at a concentration of 2.5 &mu;g/ml. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200010_fig7.png" alt="GR Antibody validated in Immunoprecipitation" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 6. Immunoprecipitation using the Diagenode monoclonal antibody against hGR </strong><br />The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 &mu;l IP reaction solution) using 5 &mu;g of the Diagenode monoclonal antibody directed against hGR (Cat. No. MAb-010-050). The IP was followed by Western blot analysis as described above </small></p>
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	'meta_title' => 'GR Antibody  - ChIP-seq Grade (C15200010) | Diagenode',
	'meta_keywords' => '',
	'meta_description' => 'GR (Glucocorticoid receptor) Monoclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB, Flow Cyt, IP and ELISA. Batch-specific data available on the website. Alternative names: NR3C1, GCCR, GCR, GRL. Sample size avaialble.',
	'modified' => '2024-11-19 16:56:51',
	'created' => '2015-06-29 14:08:20'
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	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Flow cytometry applications',
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	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Flow cytometry applications',
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	'name' => 'Coactivation of GR and NFKB alters the repertoire of their binding sites and target genes.',
	'authors' => 'Rao NA, McCalman MT, Moulos P, Francoijs KJ, Chatziioannou A, Kolisis FN, Alexis MN, Mitsiou DJ, Stunnenberg HG',
	'description' => 'Glucocorticoid receptor (GR) exerts anti-inflammatory action in part by antagonizing proinflammatory transcription factors such as the nuclear factor kappa-b (NFKB). Here, we assess the crosstalk of activated GR and RELA (p65, major NFKB component) by global identification of their binding sites and target genes. We show that coactivation of GR and p65 alters the repertoire of regulated genes and results in their association with novel sites in a mutually dependent manner. These novel sites predominantly cluster with p65 target genes that are antagonized by activated GR and vice versa. Our data show that coactivation of GR and NFKB alters signaling pathways that are regulated by each factor separately and provide insight into the networks underlying the GR and NFKB crosstalk.',
	'date' => '2011-09-01',
	'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/21750107',
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