Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'cn',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode, sample size',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'product' => array(
'Product' => array(
'id' => '3055',
'antibody_id' => '500',
'name' => 'H3.3 Antibody - ChIP-seq Grade (sample size)',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15210011-10',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000582',
'type' => 'RFR',
'search_order' => '',
'price_EUR' => '110',
'price_USD' => '120',
'price_GBP' => '105',
'price_JPY' => '17230',
'price_CNY' => '0',
'price_AUD' => '300',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'h3-3-monoclonal-antibody-10-ug',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode, sample size',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:49',
'created' => '2020-02-20 17:57:35',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '500',
'name' => 'H3.3 monoclonal antibody',
'description' => 'Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.',
'clonality' => '',
'isotype' => '',
'lot' => '002',
'concentration' => '1 µg/µl',
'reactivity' => 'Human. Other species not tested.',
'type' => 'Monoclonal',
'purity' => 'Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 μg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5-5 μg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-03-25 10:32:31',
'created' => '2017-04-06 12:25:54',
'select_label' => '500 - H3.3 monoclonal antibody (002 - 1 µg/µl - Human. Other species not tested. - Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. - Rabbit)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/h3-3-monoclonal-antibody'
)
$language = 'cn'
$meta_keywords = 'H3.3 monoclonal antibody,Histone,Diagenode, sample size'
$meta_description = 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. '
$meta_title = 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode'
$product = array(
'Product' => array(
'id' => '3055',
'antibody_id' => '500',
'name' => 'H3.3 Antibody - ChIP-seq Grade (sample size)',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant <strong>H3.3</strong>, using a KLH-conjugated synthetic peptide.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chip.jpg" alt="H3.3 Antibody ChIP Grade" caption="false" width="278" height="212" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3.3 (cat. No. C15210011) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive MYOD1 and TSH2B genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-a.jpg" alt="H3.3 Antibody ChIP-seq Grade" caption="false" width="893" height="106" /></center><br /> B.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-b.jpg" alt="H3.3 Antibody for ChIP-seq " caption="false" width="893" height="274" /></center><br /> C.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-c.jpg" alt="H3.3 Antibody for ChIP-seq assay" caption="false" width="893" height="218" /></center><br /> D.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-d.jpg" alt="H3.3 Antibody validated in ChIP-seq" caption="false" width="893" height="176" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2.ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP was performed with 1 µg of the Diagenode antibody against H3.3 (cat. No. C15210011) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3.5 Mb region of human chromosome 1 (figure 2A and B) and in two genomic regions surrounding the CCT5 and EIF2S3 positive control genes (figure 2C and D). </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-wb.jpg" alt="H3.3 Antibody validated in Western Blot" caption="false" width="201" height="260" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H3.3</strong><br /> Western blot was performed on 1 µg of recombinant histone H3.1 (lane 2) and on 1 µg of recombinant histone H3.3 (lane 1) using the Diagenode monoclonal antibody against H3.3 (cat. No. C15210011) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the socalled "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.</p>',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15210011-10',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000582',
'type' => 'RFR',
'search_order' => '',
'price_EUR' => '110',
'price_USD' => '120',
'price_GBP' => '105',
'price_JPY' => '17230',
'price_CNY' => '0',
'price_AUD' => '300',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'h3-3-monoclonal-antibody-10-ug',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode, sample size',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:49',
'created' => '2020-02-20 17:57:35',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '500',
'name' => 'H3.3 monoclonal antibody',
'description' => 'Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.',
'clonality' => '',
'isotype' => '',
'lot' => '002',
'concentration' => '1 µg/µl',
'reactivity' => 'Human. Other species not tested.',
'type' => 'Monoclonal',
'purity' => 'Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 μg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5-5 μg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-03-25 10:32:31',
'created' => '2017-04-06 12:25:54',
'select_label' => '500 - H3.3 monoclonal antibody (002 - 1 µg/µl - Human. Other species not tested. - Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. - Rabbit)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '271',
'name' => 'C15210011',
'product_id' => '2882',
'modified' => '2020-02-20 17:58:04',
'created' => '2020-02-20 17:58:04'
),
'Master' => array(
'id' => '2882',
'antibody_id' => '500',
'name' => 'H3.3 Antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant <strong>H3.3</strong>, using a KLH-conjugated synthetic peptide.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chip.jpg" alt="H3.3 Antibody ChIP Grade" caption="false" width="278" height="212" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3.3 (cat. No. C15210011) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive MYOD1 and TSH2B genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-a.jpg" alt="H3.3 Antibody ChIP-seq Grade" caption="false" width="893" height="106" /></center><br /> B.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-b.jpg" alt="H3.3 Antibody for ChIP-seq " caption="false" width="893" height="274" /></center><br /> C.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-c.jpg" alt="H3.3 Antibody for ChIP-seq assay" caption="false" width="893" height="218" /></center><br /> D.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-d.jpg" alt="H3.3 Antibody validated in ChIP-seq" caption="false" width="893" height="176" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2.ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP was performed with 1 µg of the Diagenode antibody against H3.3 (cat. No. C15210011) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3.5 Mb region of human chromosome 1 (figure 2A and B) and in two genomic regions surrounding the CCT5 and EIF2S3 positive control genes (figure 2C and D). </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-wb.jpg" alt="H3.3 Antibody validated in Western Blot" caption="false" width="201" height="260" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H3.3</strong><br /> Western blot was performed on 1 µg of recombinant histone H3.1 (lane 2) and on 1 µg of recombinant histone H3.3 (lane 1) using the Diagenode monoclonal antibody against H3.3 (cat. No. C15210011) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the socalled "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.</p>',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15210011',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000526',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '435',
'price_USD' => '440',
'price_GBP' => '395',
'price_JPY' => '68145',
'price_CNY' => '',
'price_AUD' => '1100',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'March 16, 2017',
'slug' => 'h3-3-monoclonal-antibody',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:36',
'created' => '2017-04-06 12:28:50'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '42',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-seq (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-seq-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications',
'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode',
'modified' => '2016-01-20 11:06:19',
'created' => '2015-10-20 11:44:45',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '127',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-grade antibodies',
'description' => '<div class="row">
<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode',
'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
'modified' => '2024-11-19 17:27:07',
'created' => '2017-02-14 11:16:04',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '17',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-seq grade antibodies',
'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode’s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-seq-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)',
'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:22',
'created' => '2015-02-16 02:24:01',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 2 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 3 => array(
'id' => '102',
'position' => '1',
'parent_id' => '4',
'name' => 'Sample size antibodies',
'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => true,
'is_antibody' => true,
'slug' => 'sample-size-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
'meta_title' => 'Sample-size Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:05',
'created' => '2015-10-27 12:13:34',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 4 => array(
'id' => '111',
'position' => '40',
'parent_id' => '4',
'name' => 'Histone antibodies',
'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p>
<p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p>
<p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>”, analogous to the genetic code.</p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p>
<p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p>
<p>Diagenode’s collection includes antibodies recognizing:</p>
<ul>
<li><strong>Histone H1 variants</strong></li>
<li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li>
<li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li>
<li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li>
<li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li>
</ul>
<p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p>
<p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> Expert technical support</span></li>
<li><span style="font-weight: 400;"> Sample sizes available</span></li>
<li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li>
</ul>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'histone-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Histone, antibody, histone h1, histone h2, histone h3, histone h4',
'meta_description' => 'Polyclonal and Monoclonal Antibodies against Histones and their modifications validated for many applications, including Chromatin Immunoprecipitation (ChIP) and ChIP-Sequencing (ChIP-seq)',
'meta_title' => 'Histone and Modified Histone Antibodies | Diagenode',
'modified' => '2020-09-17 13:34:56',
'created' => '2016-04-01 16:01:32',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '953',
'name' => 'H3.3 monoclonal antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant H3.3, using a KLH-conjugated synthetic peptide.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/h3-3-monoclonal-antibody.pdf',
'slug' => 'h3-3-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-05-04 16:28:43',
'created' => '2017-05-04 16:28:43',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1783',
'name' => 'product/antibodies/chipseq-grade-ab-icon.png',
'alt' => 'ChIP-seq Grade',
'modified' => '2020-11-27 07:04:40',
'created' => '2018-03-15 15:54:09',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4174',
'name' => 'Dynamic association of the H3K64 trimethylation mark with genes encodingexported proteins in Plasmodium falciparum.',
'authors' => 'Jabeena, C A et al.',
'description' => '<p>Epigenetic modifications have emerged as critical regulators of virulence genes and stage-specific gene expression in Plasmodium falciparum. However, the specific roles of histone core epigenetic modifications in regulating the stage-specific gene expression are not well understood. In this study, we report an unconventional trimethylation at lysine 64 on histone 3 (H3K64me3) and characterize its functional relevance in P. falciparum. We show that PfSET4 and PfSET5 proteins of P. falciparum methylate H3K64 and that they prefer the nucleosome as a substrate over free histone 3 proteins. Structural analysis of PfSET5 revealed that it interacts with the nucleosome as a dimer. The H3K64me3 mark is dynamic, being enriched in the ring and trophozoite stages and drastically reduced in schizont stages. Stage-specific global ChIP-sequencing analysis of the H3K64me3 mark revealed the selective enrichment of this methyl mark on the genes of exported family proteins in the ring and trophozoite stages, and a significant reduction of the same in the schizont stages. Collectively, our data identify a novel epigenetic mark that are associated with the subset of genes encoding for exported proteins which may regulate their expression in different stages of P. falciparum.</p>',
'date' => '2021-04-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33839154',
'doi' => '10.1016/j.jbc.2021.100614',
'modified' => '2021-12-21 16:07:05',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '2998',
'name' => 'H3.3 Antibody SDS GB en',
'language' => 'en',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-GB-en-GHS_1_0.pdf',
'countries' => 'GB',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '3000',
'name' => 'H3.3 Antibody SDS US en',
'language' => 'en',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-US-en-GHS_1_0.pdf',
'countries' => 'US',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '2995',
'name' => 'H3.3 Antibody SDS DE de',
'language' => 'de',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-DE-de-GHS_1_0.pdf',
'countries' => 'DE',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '2999',
'name' => 'H3.3 Antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-JP-ja-GHS_1_0.pdf',
'countries' => 'JP',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '2994',
'name' => 'H3.3 Antibody SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '2993',
'name' => 'H3.3 Antibody SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-BE-fr-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '2997',
'name' => 'H3.3 Antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-FR-fr-GHS_1_0.pdf',
'countries' => 'FR',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '2996',
'name' => 'H3.3 Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/h3-3-monoclonal-antibody'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '2882',
'antibody_id' => '500',
'name' => 'H3.3 Antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant <strong>H3.3</strong>, using a KLH-conjugated synthetic peptide.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chip.jpg" alt="H3.3 Antibody ChIP Grade" caption="false" width="278" height="212" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3.3 (cat. No. C15210011) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive MYOD1 and TSH2B genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-a.jpg" alt="H3.3 Antibody ChIP-seq Grade" caption="false" width="893" height="106" /></center><br /> B.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-b.jpg" alt="H3.3 Antibody for ChIP-seq " caption="false" width="893" height="274" /></center><br /> C.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-c.jpg" alt="H3.3 Antibody for ChIP-seq assay" caption="false" width="893" height="218" /></center><br /> D.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-d.jpg" alt="H3.3 Antibody validated in ChIP-seq" caption="false" width="893" height="176" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2.ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP was performed with 1 µg of the Diagenode antibody against H3.3 (cat. No. C15210011) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3.5 Mb region of human chromosome 1 (figure 2A and B) and in two genomic regions surrounding the CCT5 and EIF2S3 positive control genes (figure 2C and D). </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-wb.jpg" alt="H3.3 Antibody validated in Western Blot" caption="false" width="201" height="260" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H3.3</strong><br /> Western blot was performed on 1 µg of recombinant histone H3.1 (lane 2) and on 1 µg of recombinant histone H3.3 (lane 1) using the Diagenode monoclonal antibody against H3.3 (cat. No. C15210011) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the socalled "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.</p>',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15210011',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000526',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '435',
'price_USD' => '440',
'price_GBP' => '395',
'price_JPY' => '68145',
'price_CNY' => '',
'price_AUD' => '1100',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'March 16, 2017',
'slug' => 'h3-3-monoclonal-antibody',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:36',
'created' => '2017-04-06 12:28:50'
)
)
$pro = array(
'id' => '3055',
'antibody_id' => '500',
'name' => 'H3.3 Antibody (sample size)',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15210011-10',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000582',
'type' => 'RFR',
'search_order' => '',
'price_EUR' => '110',
'price_USD' => '120',
'price_GBP' => '105',
'price_JPY' => '17230',
'price_CNY' => '0',
'price_AUD' => '300',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'h3-3-monoclonal-antibody-10-ug',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode, sample size',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:49',
'created' => '2020-02-20 17:57:35',
'ProductsGroup' => array(
'id' => '306',
'product_id' => '3055',
'group_id' => '271'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2882',
'antibody_id' => '500',
'name' => 'H3.3 Antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant <strong>H3.3</strong>, using a KLH-conjugated synthetic peptide.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chip.jpg" alt="H3.3 Antibody ChIP Grade" caption="false" width="278" height="212" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3.3 (cat. No. C15210011) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive MYOD1 and TSH2B genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-a.jpg" alt="H3.3 Antibody ChIP-seq Grade" caption="false" width="893" height="106" /></center><br /> B.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-b.jpg" alt="H3.3 Antibody for ChIP-seq " caption="false" width="893" height="274" /></center><br /> C.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-c.jpg" alt="H3.3 Antibody for ChIP-seq assay" caption="false" width="893" height="218" /></center><br /> D.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-d.jpg" alt="H3.3 Antibody validated in ChIP-seq" caption="false" width="893" height="176" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2.ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP was performed with 1 µg of the Diagenode antibody against H3.3 (cat. No. C15210011) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3.5 Mb region of human chromosome 1 (figure 2A and B) and in two genomic regions surrounding the CCT5 and EIF2S3 positive control genes (figure 2C and D). </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-wb.jpg" alt="H3.3 Antibody validated in Western Blot" caption="false" width="201" height="260" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H3.3</strong><br /> Western blot was performed on 1 µg of recombinant histone H3.1 (lane 2) and on 1 µg of recombinant histone H3.3 (lane 1) using the Diagenode monoclonal antibody against H3.3 (cat. No. C15210011) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the socalled "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.</p>',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15210011',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000526',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '435',
'price_USD' => '440',
'price_GBP' => '395',
'price_JPY' => '68145',
'price_CNY' => '',
'price_AUD' => '1100',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'March 16, 2017',
'slug' => 'h3-3-monoclonal-antibody',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:36',
'created' => '2017-04-06 12:28:50'
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
'id' => '5123',
'product_id' => '3055',
'application_id' => '43'
)
)
$slugs = array(
(int) 0 => 'chip-qpcr-antibodies'
)
$applications = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'locale' => 'zho'
)
$description = ''
$name = 'ChIP-qPCR (ab)'
$document = array(
'id' => '953',
'name' => 'H3.3 monoclonal antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant H3.3, using a KLH-conjugated synthetic peptide.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/h3-3-monoclonal-antibody.pdf',
'slug' => 'h3-3-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-05-04 16:28:43',
'created' => '2017-05-04 16:28:43',
'ProductsDocument' => array(
'id' => '2860',
'product_id' => '3055',
'document_id' => '953'
)
)
$sds = array(
'id' => '2996',
'name' => 'H3.3 Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
'id' => '4912',
'product_id' => '3055',
'safety_sheet_id' => '2996'
)
)
$publication = array(
'id' => '4174',
'name' => 'Dynamic association of the H3K64 trimethylation mark with genes encodingexported proteins in Plasmodium falciparum.',
'authors' => 'Jabeena, C A et al.',
'description' => '<p>Epigenetic modifications have emerged as critical regulators of virulence genes and stage-specific gene expression in Plasmodium falciparum. However, the specific roles of histone core epigenetic modifications in regulating the stage-specific gene expression are not well understood. In this study, we report an unconventional trimethylation at lysine 64 on histone 3 (H3K64me3) and characterize its functional relevance in P. falciparum. We show that PfSET4 and PfSET5 proteins of P. falciparum methylate H3K64 and that they prefer the nucleosome as a substrate over free histone 3 proteins. Structural analysis of PfSET5 revealed that it interacts with the nucleosome as a dimer. The H3K64me3 mark is dynamic, being enriched in the ring and trophozoite stages and drastically reduced in schizont stages. Stage-specific global ChIP-sequencing analysis of the H3K64me3 mark revealed the selective enrichment of this methyl mark on the genes of exported family proteins in the ring and trophozoite stages, and a significant reduction of the same in the schizont stages. Collectively, our data identify a novel epigenetic mark that are associated with the subset of genes encoding for exported proteins which may regulate their expression in different stages of P. falciparum.</p>',
'date' => '2021-04-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33839154',
'doi' => '10.1016/j.jbc.2021.100614',
'modified' => '2021-12-21 16:07:05',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
'id' => '5368',
'product_id' => '3055',
'publication_id' => '4174'
)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/33839154" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'cn',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode, sample size',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'product' => array(
'Product' => array(
'id' => '3055',
'antibody_id' => '500',
'name' => 'H3.3 Antibody - ChIP-seq Grade (sample size)',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15210011-10',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000582',
'type' => 'RFR',
'search_order' => '',
'price_EUR' => '110',
'price_USD' => '120',
'price_GBP' => '105',
'price_JPY' => '17230',
'price_CNY' => '0',
'price_AUD' => '300',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'h3-3-monoclonal-antibody-10-ug',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode, sample size',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:49',
'created' => '2020-02-20 17:57:35',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '500',
'name' => 'H3.3 monoclonal antibody',
'description' => 'Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.',
'clonality' => '',
'isotype' => '',
'lot' => '002',
'concentration' => '1 µg/µl',
'reactivity' => 'Human. Other species not tested.',
'type' => 'Monoclonal',
'purity' => 'Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 μg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5-5 μg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-03-25 10:32:31',
'created' => '2017-04-06 12:25:54',
'select_label' => '500 - H3.3 monoclonal antibody (002 - 1 µg/µl - Human. Other species not tested. - Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. - Rabbit)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/h3-3-monoclonal-antibody'
)
$language = 'cn'
$meta_keywords = 'H3.3 monoclonal antibody,Histone,Diagenode, sample size'
$meta_description = 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. '
$meta_title = 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode'
$product = array(
'Product' => array(
'id' => '3055',
'antibody_id' => '500',
'name' => 'H3.3 Antibody - ChIP-seq Grade (sample size)',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant <strong>H3.3</strong>, using a KLH-conjugated synthetic peptide.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chip.jpg" alt="H3.3 Antibody ChIP Grade" caption="false" width="278" height="212" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3.3 (cat. No. C15210011) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive MYOD1 and TSH2B genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-a.jpg" alt="H3.3 Antibody ChIP-seq Grade" caption="false" width="893" height="106" /></center><br /> B.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-b.jpg" alt="H3.3 Antibody for ChIP-seq " caption="false" width="893" height="274" /></center><br /> C.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-c.jpg" alt="H3.3 Antibody for ChIP-seq assay" caption="false" width="893" height="218" /></center><br /> D.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-d.jpg" alt="H3.3 Antibody validated in ChIP-seq" caption="false" width="893" height="176" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2.ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP was performed with 1 µg of the Diagenode antibody against H3.3 (cat. No. C15210011) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3.5 Mb region of human chromosome 1 (figure 2A and B) and in two genomic regions surrounding the CCT5 and EIF2S3 positive control genes (figure 2C and D). </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-wb.jpg" alt="H3.3 Antibody validated in Western Blot" caption="false" width="201" height="260" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H3.3</strong><br /> Western blot was performed on 1 µg of recombinant histone H3.1 (lane 2) and on 1 µg of recombinant histone H3.3 (lane 1) using the Diagenode monoclonal antibody against H3.3 (cat. No. C15210011) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the socalled "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.</p>',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15210011-10',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000582',
'type' => 'RFR',
'search_order' => '',
'price_EUR' => '110',
'price_USD' => '120',
'price_GBP' => '105',
'price_JPY' => '17230',
'price_CNY' => '0',
'price_AUD' => '300',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'h3-3-monoclonal-antibody-10-ug',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode, sample size',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:49',
'created' => '2020-02-20 17:57:35',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '500',
'name' => 'H3.3 monoclonal antibody',
'description' => 'Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.',
'clonality' => '',
'isotype' => '',
'lot' => '002',
'concentration' => '1 µg/µl',
'reactivity' => 'Human. Other species not tested.',
'type' => 'Monoclonal',
'purity' => 'Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 μg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5-5 μg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-03-25 10:32:31',
'created' => '2017-04-06 12:25:54',
'select_label' => '500 - H3.3 monoclonal antibody (002 - 1 µg/µl - Human. Other species not tested. - Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. - Rabbit)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '271',
'name' => 'C15210011',
'product_id' => '2882',
'modified' => '2020-02-20 17:58:04',
'created' => '2020-02-20 17:58:04'
),
'Master' => array(
'id' => '2882',
'antibody_id' => '500',
'name' => 'H3.3 Antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant <strong>H3.3</strong>, using a KLH-conjugated synthetic peptide.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chip.jpg" alt="H3.3 Antibody ChIP Grade" caption="false" width="278" height="212" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3.3 (cat. No. C15210011) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive MYOD1 and TSH2B genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-a.jpg" alt="H3.3 Antibody ChIP-seq Grade" caption="false" width="893" height="106" /></center><br /> B.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-b.jpg" alt="H3.3 Antibody for ChIP-seq " caption="false" width="893" height="274" /></center><br /> C.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-c.jpg" alt="H3.3 Antibody for ChIP-seq assay" caption="false" width="893" height="218" /></center><br /> D.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-d.jpg" alt="H3.3 Antibody validated in ChIP-seq" caption="false" width="893" height="176" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2.ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP was performed with 1 µg of the Diagenode antibody against H3.3 (cat. No. C15210011) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3.5 Mb region of human chromosome 1 (figure 2A and B) and in two genomic regions surrounding the CCT5 and EIF2S3 positive control genes (figure 2C and D). </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-wb.jpg" alt="H3.3 Antibody validated in Western Blot" caption="false" width="201" height="260" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H3.3</strong><br /> Western blot was performed on 1 µg of recombinant histone H3.1 (lane 2) and on 1 µg of recombinant histone H3.3 (lane 1) using the Diagenode monoclonal antibody against H3.3 (cat. No. C15210011) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the socalled "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.</p>',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15210011',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000526',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '435',
'price_USD' => '440',
'price_GBP' => '395',
'price_JPY' => '68145',
'price_CNY' => '',
'price_AUD' => '1100',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'March 16, 2017',
'slug' => 'h3-3-monoclonal-antibody',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:36',
'created' => '2017-04-06 12:28:50'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '42',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-seq (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-seq-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications',
'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode',
'modified' => '2016-01-20 11:06:19',
'created' => '2015-10-20 11:44:45',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '127',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-grade antibodies',
'description' => '<div class="row">
<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode',
'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
'modified' => '2024-11-19 17:27:07',
'created' => '2017-02-14 11:16:04',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '17',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-seq grade antibodies',
'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode’s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-seq-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)',
'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:22',
'created' => '2015-02-16 02:24:01',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 2 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 3 => array(
'id' => '102',
'position' => '1',
'parent_id' => '4',
'name' => 'Sample size antibodies',
'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => true,
'is_antibody' => true,
'slug' => 'sample-size-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
'meta_title' => 'Sample-size Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:05',
'created' => '2015-10-27 12:13:34',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 4 => array(
'id' => '111',
'position' => '40',
'parent_id' => '4',
'name' => 'Histone antibodies',
'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p>
<p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p>
<p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>”, analogous to the genetic code.</p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p>
<p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p>
<p>Diagenode’s collection includes antibodies recognizing:</p>
<ul>
<li><strong>Histone H1 variants</strong></li>
<li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li>
<li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li>
<li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li>
<li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li>
</ul>
<p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p>
<p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> Expert technical support</span></li>
<li><span style="font-weight: 400;"> Sample sizes available</span></li>
<li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li>
</ul>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'histone-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Histone, antibody, histone h1, histone h2, histone h3, histone h4',
'meta_description' => 'Polyclonal and Monoclonal Antibodies against Histones and their modifications validated for many applications, including Chromatin Immunoprecipitation (ChIP) and ChIP-Sequencing (ChIP-seq)',
'meta_title' => 'Histone and Modified Histone Antibodies | Diagenode',
'modified' => '2020-09-17 13:34:56',
'created' => '2016-04-01 16:01:32',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '953',
'name' => 'H3.3 monoclonal antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant H3.3, using a KLH-conjugated synthetic peptide.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/h3-3-monoclonal-antibody.pdf',
'slug' => 'h3-3-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-05-04 16:28:43',
'created' => '2017-05-04 16:28:43',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1783',
'name' => 'product/antibodies/chipseq-grade-ab-icon.png',
'alt' => 'ChIP-seq Grade',
'modified' => '2020-11-27 07:04:40',
'created' => '2018-03-15 15:54:09',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4174',
'name' => 'Dynamic association of the H3K64 trimethylation mark with genes encodingexported proteins in Plasmodium falciparum.',
'authors' => 'Jabeena, C A et al.',
'description' => '<p>Epigenetic modifications have emerged as critical regulators of virulence genes and stage-specific gene expression in Plasmodium falciparum. However, the specific roles of histone core epigenetic modifications in regulating the stage-specific gene expression are not well understood. In this study, we report an unconventional trimethylation at lysine 64 on histone 3 (H3K64me3) and characterize its functional relevance in P. falciparum. We show that PfSET4 and PfSET5 proteins of P. falciparum methylate H3K64 and that they prefer the nucleosome as a substrate over free histone 3 proteins. Structural analysis of PfSET5 revealed that it interacts with the nucleosome as a dimer. The H3K64me3 mark is dynamic, being enriched in the ring and trophozoite stages and drastically reduced in schizont stages. Stage-specific global ChIP-sequencing analysis of the H3K64me3 mark revealed the selective enrichment of this methyl mark on the genes of exported family proteins in the ring and trophozoite stages, and a significant reduction of the same in the schizont stages. Collectively, our data identify a novel epigenetic mark that are associated with the subset of genes encoding for exported proteins which may regulate their expression in different stages of P. falciparum.</p>',
'date' => '2021-04-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33839154',
'doi' => '10.1016/j.jbc.2021.100614',
'modified' => '2021-12-21 16:07:05',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '2998',
'name' => 'H3.3 Antibody SDS GB en',
'language' => 'en',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-GB-en-GHS_1_0.pdf',
'countries' => 'GB',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '3000',
'name' => 'H3.3 Antibody SDS US en',
'language' => 'en',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-US-en-GHS_1_0.pdf',
'countries' => 'US',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '2995',
'name' => 'H3.3 Antibody SDS DE de',
'language' => 'de',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-DE-de-GHS_1_0.pdf',
'countries' => 'DE',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '2999',
'name' => 'H3.3 Antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-JP-ja-GHS_1_0.pdf',
'countries' => 'JP',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '2994',
'name' => 'H3.3 Antibody SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '2993',
'name' => 'H3.3 Antibody SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-BE-fr-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '2997',
'name' => 'H3.3 Antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-FR-fr-GHS_1_0.pdf',
'countries' => 'FR',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '2996',
'name' => 'H3.3 Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/h3-3-monoclonal-antibody'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '2882',
'antibody_id' => '500',
'name' => 'H3.3 Antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant <strong>H3.3</strong>, using a KLH-conjugated synthetic peptide.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chip.jpg" alt="H3.3 Antibody ChIP Grade" caption="false" width="278" height="212" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3.3 (cat. No. C15210011) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive MYOD1 and TSH2B genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-a.jpg" alt="H3.3 Antibody ChIP-seq Grade" caption="false" width="893" height="106" /></center><br /> B.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-b.jpg" alt="H3.3 Antibody for ChIP-seq " caption="false" width="893" height="274" /></center><br /> C.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-c.jpg" alt="H3.3 Antibody for ChIP-seq assay" caption="false" width="893" height="218" /></center><br /> D.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-d.jpg" alt="H3.3 Antibody validated in ChIP-seq" caption="false" width="893" height="176" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2.ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP was performed with 1 µg of the Diagenode antibody against H3.3 (cat. No. C15210011) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3.5 Mb region of human chromosome 1 (figure 2A and B) and in two genomic regions surrounding the CCT5 and EIF2S3 positive control genes (figure 2C and D). </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-wb.jpg" alt="H3.3 Antibody validated in Western Blot" caption="false" width="201" height="260" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H3.3</strong><br /> Western blot was performed on 1 µg of recombinant histone H3.1 (lane 2) and on 1 µg of recombinant histone H3.3 (lane 1) using the Diagenode monoclonal antibody against H3.3 (cat. No. C15210011) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the socalled "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.</p>',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15210011',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000526',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '435',
'price_USD' => '440',
'price_GBP' => '395',
'price_JPY' => '68145',
'price_CNY' => '',
'price_AUD' => '1100',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'March 16, 2017',
'slug' => 'h3-3-monoclonal-antibody',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:36',
'created' => '2017-04-06 12:28:50'
)
)
$pro = array(
'id' => '3055',
'antibody_id' => '500',
'name' => 'H3.3 Antibody (sample size)',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15210011-10',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000582',
'type' => 'RFR',
'search_order' => '',
'price_EUR' => '110',
'price_USD' => '120',
'price_GBP' => '105',
'price_JPY' => '17230',
'price_CNY' => '0',
'price_AUD' => '300',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'h3-3-monoclonal-antibody-10-ug',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode, sample size',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:49',
'created' => '2020-02-20 17:57:35',
'ProductsGroup' => array(
'id' => '306',
'product_id' => '3055',
'group_id' => '271'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2882',
'antibody_id' => '500',
'name' => 'H3.3 Antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant <strong>H3.3</strong>, using a KLH-conjugated synthetic peptide.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chip.jpg" alt="H3.3 Antibody ChIP Grade" caption="false" width="278" height="212" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3.3 (cat. No. C15210011) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive MYOD1 and TSH2B genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-a.jpg" alt="H3.3 Antibody ChIP-seq Grade" caption="false" width="893" height="106" /></center><br /> B.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-b.jpg" alt="H3.3 Antibody for ChIP-seq " caption="false" width="893" height="274" /></center><br /> C.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-c.jpg" alt="H3.3 Antibody for ChIP-seq assay" caption="false" width="893" height="218" /></center><br /> D.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-d.jpg" alt="H3.3 Antibody validated in ChIP-seq" caption="false" width="893" height="176" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2.ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP was performed with 1 µg of the Diagenode antibody against H3.3 (cat. No. C15210011) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3.5 Mb region of human chromosome 1 (figure 2A and B) and in two genomic regions surrounding the CCT5 and EIF2S3 positive control genes (figure 2C and D). </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-wb.jpg" alt="H3.3 Antibody validated in Western Blot" caption="false" width="201" height="260" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H3.3</strong><br /> Western blot was performed on 1 µg of recombinant histone H3.1 (lane 2) and on 1 µg of recombinant histone H3.3 (lane 1) using the Diagenode monoclonal antibody against H3.3 (cat. No. C15210011) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the socalled "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.</p>',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15210011',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000526',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '435',
'price_USD' => '440',
'price_GBP' => '395',
'price_JPY' => '68145',
'price_CNY' => '',
'price_AUD' => '1100',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'March 16, 2017',
'slug' => 'h3-3-monoclonal-antibody',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:36',
'created' => '2017-04-06 12:28:50'
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
'id' => '5123',
'product_id' => '3055',
'application_id' => '43'
)
)
$slugs = array(
(int) 0 => 'chip-qpcr-antibodies'
)
$applications = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'locale' => 'zho'
)
$description = ''
$name = 'ChIP-qPCR (ab)'
$document = array(
'id' => '953',
'name' => 'H3.3 monoclonal antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant H3.3, using a KLH-conjugated synthetic peptide.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/h3-3-monoclonal-antibody.pdf',
'slug' => 'h3-3-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-05-04 16:28:43',
'created' => '2017-05-04 16:28:43',
'ProductsDocument' => array(
'id' => '2860',
'product_id' => '3055',
'document_id' => '953'
)
)
$sds = array(
'id' => '2996',
'name' => 'H3.3 Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
'id' => '4912',
'product_id' => '3055',
'safety_sheet_id' => '2996'
)
)
$publication = array(
'id' => '4174',
'name' => 'Dynamic association of the H3K64 trimethylation mark with genes encodingexported proteins in Plasmodium falciparum.',
'authors' => 'Jabeena, C A et al.',
'description' => '<p>Epigenetic modifications have emerged as critical regulators of virulence genes and stage-specific gene expression in Plasmodium falciparum. However, the specific roles of histone core epigenetic modifications in regulating the stage-specific gene expression are not well understood. In this study, we report an unconventional trimethylation at lysine 64 on histone 3 (H3K64me3) and characterize its functional relevance in P. falciparum. We show that PfSET4 and PfSET5 proteins of P. falciparum methylate H3K64 and that they prefer the nucleosome as a substrate over free histone 3 proteins. Structural analysis of PfSET5 revealed that it interacts with the nucleosome as a dimer. The H3K64me3 mark is dynamic, being enriched in the ring and trophozoite stages and drastically reduced in schizont stages. Stage-specific global ChIP-sequencing analysis of the H3K64me3 mark revealed the selective enrichment of this methyl mark on the genes of exported family proteins in the ring and trophozoite stages, and a significant reduction of the same in the schizont stages. Collectively, our data identify a novel epigenetic mark that are associated with the subset of genes encoding for exported proteins which may regulate their expression in different stages of P. falciparum.</p>',
'date' => '2021-04-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33839154',
'doi' => '10.1016/j.jbc.2021.100614',
'modified' => '2021-12-21 16:07:05',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
'id' => '5368',
'product_id' => '3055',
'publication_id' => '4174'
)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/33839154" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'cn',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode, sample size',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'product' => array(
'Product' => array(
'id' => '3055',
'antibody_id' => '500',
'name' => 'H3.3 Antibody - ChIP-seq Grade (sample size)',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15210011-10',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000582',
'type' => 'RFR',
'search_order' => '',
'price_EUR' => '110',
'price_USD' => '120',
'price_GBP' => '105',
'price_JPY' => '17230',
'price_CNY' => '0',
'price_AUD' => '300',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'h3-3-monoclonal-antibody-10-ug',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode, sample size',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:49',
'created' => '2020-02-20 17:57:35',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '500',
'name' => 'H3.3 monoclonal antibody',
'description' => 'Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.',
'clonality' => '',
'isotype' => '',
'lot' => '002',
'concentration' => '1 µg/µl',
'reactivity' => 'Human. Other species not tested.',
'type' => 'Monoclonal',
'purity' => 'Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 μg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5-5 μg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-03-25 10:32:31',
'created' => '2017-04-06 12:25:54',
'select_label' => '500 - H3.3 monoclonal antibody (002 - 1 µg/µl - Human. Other species not tested. - Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. - Rabbit)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/h3-3-monoclonal-antibody'
)
$language = 'cn'
$meta_keywords = 'H3.3 monoclonal antibody,Histone,Diagenode, sample size'
$meta_description = 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. '
$meta_title = 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode'
$product = array(
'Product' => array(
'id' => '3055',
'antibody_id' => '500',
'name' => 'H3.3 Antibody - ChIP-seq Grade (sample size)',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant <strong>H3.3</strong>, using a KLH-conjugated synthetic peptide.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chip.jpg" alt="H3.3 Antibody ChIP Grade" caption="false" width="278" height="212" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3.3 (cat. No. C15210011) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive MYOD1 and TSH2B genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-a.jpg" alt="H3.3 Antibody ChIP-seq Grade" caption="false" width="893" height="106" /></center><br /> B.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-b.jpg" alt="H3.3 Antibody for ChIP-seq " caption="false" width="893" height="274" /></center><br /> C.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-c.jpg" alt="H3.3 Antibody for ChIP-seq assay" caption="false" width="893" height="218" /></center><br /> D.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-d.jpg" alt="H3.3 Antibody validated in ChIP-seq" caption="false" width="893" height="176" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2.ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP was performed with 1 µg of the Diagenode antibody against H3.3 (cat. No. C15210011) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3.5 Mb region of human chromosome 1 (figure 2A and B) and in two genomic regions surrounding the CCT5 and EIF2S3 positive control genes (figure 2C and D). </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-wb.jpg" alt="H3.3 Antibody validated in Western Blot" caption="false" width="201" height="260" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H3.3</strong><br /> Western blot was performed on 1 µg of recombinant histone H3.1 (lane 2) and on 1 µg of recombinant histone H3.3 (lane 1) using the Diagenode monoclonal antibody against H3.3 (cat. No. C15210011) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the socalled "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.</p>',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15210011-10',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000582',
'type' => 'RFR',
'search_order' => '',
'price_EUR' => '110',
'price_USD' => '120',
'price_GBP' => '105',
'price_JPY' => '17230',
'price_CNY' => '0',
'price_AUD' => '300',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'h3-3-monoclonal-antibody-10-ug',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode, sample size',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:49',
'created' => '2020-02-20 17:57:35',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '500',
'name' => 'H3.3 monoclonal antibody',
'description' => 'Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.',
'clonality' => '',
'isotype' => '',
'lot' => '002',
'concentration' => '1 µg/µl',
'reactivity' => 'Human. Other species not tested.',
'type' => 'Monoclonal',
'purity' => 'Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 μg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5-5 μg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-03-25 10:32:31',
'created' => '2017-04-06 12:25:54',
'select_label' => '500 - H3.3 monoclonal antibody (002 - 1 µg/µl - Human. Other species not tested. - Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. - Rabbit)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '271',
'name' => 'C15210011',
'product_id' => '2882',
'modified' => '2020-02-20 17:58:04',
'created' => '2020-02-20 17:58:04'
),
'Master' => array(
'id' => '2882',
'antibody_id' => '500',
'name' => 'H3.3 Antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant <strong>H3.3</strong>, using a KLH-conjugated synthetic peptide.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chip.jpg" alt="H3.3 Antibody ChIP Grade" caption="false" width="278" height="212" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3.3 (cat. No. C15210011) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive MYOD1 and TSH2B genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-a.jpg" alt="H3.3 Antibody ChIP-seq Grade" caption="false" width="893" height="106" /></center><br /> B.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-b.jpg" alt="H3.3 Antibody for ChIP-seq " caption="false" width="893" height="274" /></center><br /> C.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-c.jpg" alt="H3.3 Antibody for ChIP-seq assay" caption="false" width="893" height="218" /></center><br /> D.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-d.jpg" alt="H3.3 Antibody validated in ChIP-seq" caption="false" width="893" height="176" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2.ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP was performed with 1 µg of the Diagenode antibody against H3.3 (cat. No. C15210011) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3.5 Mb region of human chromosome 1 (figure 2A and B) and in two genomic regions surrounding the CCT5 and EIF2S3 positive control genes (figure 2C and D). </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-wb.jpg" alt="H3.3 Antibody validated in Western Blot" caption="false" width="201" height="260" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H3.3</strong><br /> Western blot was performed on 1 µg of recombinant histone H3.1 (lane 2) and on 1 µg of recombinant histone H3.3 (lane 1) using the Diagenode monoclonal antibody against H3.3 (cat. No. C15210011) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the socalled "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.</p>',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15210011',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000526',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '435',
'price_USD' => '440',
'price_GBP' => '395',
'price_JPY' => '68145',
'price_CNY' => '',
'price_AUD' => '1100',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'March 16, 2017',
'slug' => 'h3-3-monoclonal-antibody',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:36',
'created' => '2017-04-06 12:28:50'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '42',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-seq (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-seq-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications',
'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode',
'modified' => '2016-01-20 11:06:19',
'created' => '2015-10-20 11:44:45',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '127',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-grade antibodies',
'description' => '<div class="row">
<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode',
'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
'modified' => '2024-11-19 17:27:07',
'created' => '2017-02-14 11:16:04',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '17',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-seq grade antibodies',
'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode’s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-seq-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)',
'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:22',
'created' => '2015-02-16 02:24:01',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 2 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 3 => array(
'id' => '102',
'position' => '1',
'parent_id' => '4',
'name' => 'Sample size antibodies',
'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => true,
'is_antibody' => true,
'slug' => 'sample-size-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
'meta_title' => 'Sample-size Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:05',
'created' => '2015-10-27 12:13:34',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 4 => array(
'id' => '111',
'position' => '40',
'parent_id' => '4',
'name' => 'Histone antibodies',
'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p>
<p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p>
<p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>”, analogous to the genetic code.</p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p>
<p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p>
<p>Diagenode’s collection includes antibodies recognizing:</p>
<ul>
<li><strong>Histone H1 variants</strong></li>
<li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li>
<li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li>
<li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li>
<li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li>
</ul>
<p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p>
<p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> Expert technical support</span></li>
<li><span style="font-weight: 400;"> Sample sizes available</span></li>
<li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li>
</ul>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'histone-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Histone, antibody, histone h1, histone h2, histone h3, histone h4',
'meta_description' => 'Polyclonal and Monoclonal Antibodies against Histones and their modifications validated for many applications, including Chromatin Immunoprecipitation (ChIP) and ChIP-Sequencing (ChIP-seq)',
'meta_title' => 'Histone and Modified Histone Antibodies | Diagenode',
'modified' => '2020-09-17 13:34:56',
'created' => '2016-04-01 16:01:32',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '953',
'name' => 'H3.3 monoclonal antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant H3.3, using a KLH-conjugated synthetic peptide.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/h3-3-monoclonal-antibody.pdf',
'slug' => 'h3-3-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-05-04 16:28:43',
'created' => '2017-05-04 16:28:43',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1783',
'name' => 'product/antibodies/chipseq-grade-ab-icon.png',
'alt' => 'ChIP-seq Grade',
'modified' => '2020-11-27 07:04:40',
'created' => '2018-03-15 15:54:09',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4174',
'name' => 'Dynamic association of the H3K64 trimethylation mark with genes encodingexported proteins in Plasmodium falciparum.',
'authors' => 'Jabeena, C A et al.',
'description' => '<p>Epigenetic modifications have emerged as critical regulators of virulence genes and stage-specific gene expression in Plasmodium falciparum. However, the specific roles of histone core epigenetic modifications in regulating the stage-specific gene expression are not well understood. In this study, we report an unconventional trimethylation at lysine 64 on histone 3 (H3K64me3) and characterize its functional relevance in P. falciparum. We show that PfSET4 and PfSET5 proteins of P. falciparum methylate H3K64 and that they prefer the nucleosome as a substrate over free histone 3 proteins. Structural analysis of PfSET5 revealed that it interacts with the nucleosome as a dimer. The H3K64me3 mark is dynamic, being enriched in the ring and trophozoite stages and drastically reduced in schizont stages. Stage-specific global ChIP-sequencing analysis of the H3K64me3 mark revealed the selective enrichment of this methyl mark on the genes of exported family proteins in the ring and trophozoite stages, and a significant reduction of the same in the schizont stages. Collectively, our data identify a novel epigenetic mark that are associated with the subset of genes encoding for exported proteins which may regulate their expression in different stages of P. falciparum.</p>',
'date' => '2021-04-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33839154',
'doi' => '10.1016/j.jbc.2021.100614',
'modified' => '2021-12-21 16:07:05',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '2998',
'name' => 'H3.3 Antibody SDS GB en',
'language' => 'en',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-GB-en-GHS_1_0.pdf',
'countries' => 'GB',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '3000',
'name' => 'H3.3 Antibody SDS US en',
'language' => 'en',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-US-en-GHS_1_0.pdf',
'countries' => 'US',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '2995',
'name' => 'H3.3 Antibody SDS DE de',
'language' => 'de',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-DE-de-GHS_1_0.pdf',
'countries' => 'DE',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '2999',
'name' => 'H3.3 Antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-JP-ja-GHS_1_0.pdf',
'countries' => 'JP',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '2994',
'name' => 'H3.3 Antibody SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '2993',
'name' => 'H3.3 Antibody SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-BE-fr-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '2997',
'name' => 'H3.3 Antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-FR-fr-GHS_1_0.pdf',
'countries' => 'FR',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '2996',
'name' => 'H3.3 Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/h3-3-monoclonal-antibody'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '2882',
'antibody_id' => '500',
'name' => 'H3.3 Antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant <strong>H3.3</strong>, using a KLH-conjugated synthetic peptide.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chip.jpg" alt="H3.3 Antibody ChIP Grade" caption="false" width="278" height="212" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3.3 (cat. No. C15210011) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive MYOD1 and TSH2B genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-a.jpg" alt="H3.3 Antibody ChIP-seq Grade" caption="false" width="893" height="106" /></center><br /> B.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-b.jpg" alt="H3.3 Antibody for ChIP-seq " caption="false" width="893" height="274" /></center><br /> C.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-c.jpg" alt="H3.3 Antibody for ChIP-seq assay" caption="false" width="893" height="218" /></center><br /> D.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-d.jpg" alt="H3.3 Antibody validated in ChIP-seq" caption="false" width="893" height="176" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2.ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP was performed with 1 µg of the Diagenode antibody against H3.3 (cat. No. C15210011) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3.5 Mb region of human chromosome 1 (figure 2A and B) and in two genomic regions surrounding the CCT5 and EIF2S3 positive control genes (figure 2C and D). </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-wb.jpg" alt="H3.3 Antibody validated in Western Blot" caption="false" width="201" height="260" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H3.3</strong><br /> Western blot was performed on 1 µg of recombinant histone H3.1 (lane 2) and on 1 µg of recombinant histone H3.3 (lane 1) using the Diagenode monoclonal antibody against H3.3 (cat. No. C15210011) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the socalled "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.</p>',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15210011',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000526',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '435',
'price_USD' => '440',
'price_GBP' => '395',
'price_JPY' => '68145',
'price_CNY' => '',
'price_AUD' => '1100',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'March 16, 2017',
'slug' => 'h3-3-monoclonal-antibody',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:36',
'created' => '2017-04-06 12:28:50'
)
)
$pro = array(
'id' => '3055',
'antibody_id' => '500',
'name' => 'H3.3 Antibody (sample size)',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15210011-10',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000582',
'type' => 'RFR',
'search_order' => '',
'price_EUR' => '110',
'price_USD' => '120',
'price_GBP' => '105',
'price_JPY' => '17230',
'price_CNY' => '0',
'price_AUD' => '300',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'h3-3-monoclonal-antibody-10-ug',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode, sample size',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:49',
'created' => '2020-02-20 17:57:35',
'ProductsGroup' => array(
'id' => '306',
'product_id' => '3055',
'group_id' => '271'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2882',
'antibody_id' => '500',
'name' => 'H3.3 Antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant <strong>H3.3</strong>, using a KLH-conjugated synthetic peptide.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chip.jpg" alt="H3.3 Antibody ChIP Grade" caption="false" width="278" height="212" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3.3 (cat. No. C15210011) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive MYOD1 and TSH2B genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-a.jpg" alt="H3.3 Antibody ChIP-seq Grade" caption="false" width="893" height="106" /></center><br /> B.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-b.jpg" alt="H3.3 Antibody for ChIP-seq " caption="false" width="893" height="274" /></center><br /> C.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-c.jpg" alt="H3.3 Antibody for ChIP-seq assay" caption="false" width="893" height="218" /></center><br /> D.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-d.jpg" alt="H3.3 Antibody validated in ChIP-seq" caption="false" width="893" height="176" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2.ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP was performed with 1 µg of the Diagenode antibody against H3.3 (cat. No. C15210011) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3.5 Mb region of human chromosome 1 (figure 2A and B) and in two genomic regions surrounding the CCT5 and EIF2S3 positive control genes (figure 2C and D). </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-wb.jpg" alt="H3.3 Antibody validated in Western Blot" caption="false" width="201" height="260" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H3.3</strong><br /> Western blot was performed on 1 µg of recombinant histone H3.1 (lane 2) and on 1 µg of recombinant histone H3.3 (lane 1) using the Diagenode monoclonal antibody against H3.3 (cat. No. C15210011) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the socalled "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.</p>',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15210011',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000526',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '435',
'price_USD' => '440',
'price_GBP' => '395',
'price_JPY' => '68145',
'price_CNY' => '',
'price_AUD' => '1100',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'March 16, 2017',
'slug' => 'h3-3-monoclonal-antibody',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:36',
'created' => '2017-04-06 12:28:50'
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
'id' => '5123',
'product_id' => '3055',
'application_id' => '43'
)
)
$slugs = array(
(int) 0 => 'chip-qpcr-antibodies'
)
$applications = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'locale' => 'zho'
)
$description = ''
$name = 'ChIP-qPCR (ab)'
$document = array(
'id' => '953',
'name' => 'H3.3 monoclonal antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant H3.3, using a KLH-conjugated synthetic peptide.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/h3-3-monoclonal-antibody.pdf',
'slug' => 'h3-3-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-05-04 16:28:43',
'created' => '2017-05-04 16:28:43',
'ProductsDocument' => array(
'id' => '2860',
'product_id' => '3055',
'document_id' => '953'
)
)
$sds = array(
'id' => '2996',
'name' => 'H3.3 Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
'id' => '4912',
'product_id' => '3055',
'safety_sheet_id' => '2996'
)
)
$publication = array(
'id' => '4174',
'name' => 'Dynamic association of the H3K64 trimethylation mark with genes encodingexported proteins in Plasmodium falciparum.',
'authors' => 'Jabeena, C A et al.',
'description' => '<p>Epigenetic modifications have emerged as critical regulators of virulence genes and stage-specific gene expression in Plasmodium falciparum. However, the specific roles of histone core epigenetic modifications in regulating the stage-specific gene expression are not well understood. In this study, we report an unconventional trimethylation at lysine 64 on histone 3 (H3K64me3) and characterize its functional relevance in P. falciparum. We show that PfSET4 and PfSET5 proteins of P. falciparum methylate H3K64 and that they prefer the nucleosome as a substrate over free histone 3 proteins. Structural analysis of PfSET5 revealed that it interacts with the nucleosome as a dimer. The H3K64me3 mark is dynamic, being enriched in the ring and trophozoite stages and drastically reduced in schizont stages. Stage-specific global ChIP-sequencing analysis of the H3K64me3 mark revealed the selective enrichment of this methyl mark on the genes of exported family proteins in the ring and trophozoite stages, and a significant reduction of the same in the schizont stages. Collectively, our data identify a novel epigenetic mark that are associated with the subset of genes encoding for exported proteins which may regulate their expression in different stages of P. falciparum.</p>',
'date' => '2021-04-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33839154',
'doi' => '10.1016/j.jbc.2021.100614',
'modified' => '2021-12-21 16:07:05',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
'id' => '5368',
'product_id' => '3055',
'publication_id' => '4174'
)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/33839154" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'cn',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode, sample size',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'product' => array(
'Product' => array(
'id' => '3055',
'antibody_id' => '500',
'name' => 'H3.3 Antibody - ChIP-seq Grade (sample size)',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15210011-10',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000582',
'type' => 'RFR',
'search_order' => '',
'price_EUR' => '110',
'price_USD' => '120',
'price_GBP' => '105',
'price_JPY' => '17230',
'price_CNY' => '0',
'price_AUD' => '300',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'h3-3-monoclonal-antibody-10-ug',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode, sample size',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:49',
'created' => '2020-02-20 17:57:35',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '500',
'name' => 'H3.3 monoclonal antibody',
'description' => 'Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.',
'clonality' => '',
'isotype' => '',
'lot' => '002',
'concentration' => '1 µg/µl',
'reactivity' => 'Human. Other species not tested.',
'type' => 'Monoclonal',
'purity' => 'Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 μg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5-5 μg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-03-25 10:32:31',
'created' => '2017-04-06 12:25:54',
'select_label' => '500 - H3.3 monoclonal antibody (002 - 1 µg/µl - Human. Other species not tested. - Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. - Rabbit)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/h3-3-monoclonal-antibody'
)
$language = 'cn'
$meta_keywords = 'H3.3 monoclonal antibody,Histone,Diagenode, sample size'
$meta_description = 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. '
$meta_title = 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode'
$product = array(
'Product' => array(
'id' => '3055',
'antibody_id' => '500',
'name' => 'H3.3 Antibody - ChIP-seq Grade (sample size)',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant <strong>H3.3</strong>, using a KLH-conjugated synthetic peptide.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chip.jpg" alt="H3.3 Antibody ChIP Grade" caption="false" width="278" height="212" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3.3 (cat. No. C15210011) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive MYOD1 and TSH2B genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-a.jpg" alt="H3.3 Antibody ChIP-seq Grade" caption="false" width="893" height="106" /></center><br /> B.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-b.jpg" alt="H3.3 Antibody for ChIP-seq " caption="false" width="893" height="274" /></center><br /> C.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-c.jpg" alt="H3.3 Antibody for ChIP-seq assay" caption="false" width="893" height="218" /></center><br /> D.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-d.jpg" alt="H3.3 Antibody validated in ChIP-seq" caption="false" width="893" height="176" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2.ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP was performed with 1 µg of the Diagenode antibody against H3.3 (cat. No. C15210011) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3.5 Mb region of human chromosome 1 (figure 2A and B) and in two genomic regions surrounding the CCT5 and EIF2S3 positive control genes (figure 2C and D). </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-wb.jpg" alt="H3.3 Antibody validated in Western Blot" caption="false" width="201" height="260" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H3.3</strong><br /> Western blot was performed on 1 µg of recombinant histone H3.1 (lane 2) and on 1 µg of recombinant histone H3.3 (lane 1) using the Diagenode monoclonal antibody against H3.3 (cat. No. C15210011) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the socalled "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.</p>',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15210011-10',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000582',
'type' => 'RFR',
'search_order' => '',
'price_EUR' => '110',
'price_USD' => '120',
'price_GBP' => '105',
'price_JPY' => '17230',
'price_CNY' => '0',
'price_AUD' => '300',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'h3-3-monoclonal-antibody-10-ug',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode, sample size',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:49',
'created' => '2020-02-20 17:57:35',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '500',
'name' => 'H3.3 monoclonal antibody',
'description' => 'Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.',
'clonality' => '',
'isotype' => '',
'lot' => '002',
'concentration' => '1 µg/µl',
'reactivity' => 'Human. Other species not tested.',
'type' => 'Monoclonal',
'purity' => 'Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 μg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5-5 μg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-03-25 10:32:31',
'created' => '2017-04-06 12:25:54',
'select_label' => '500 - H3.3 monoclonal antibody (002 - 1 µg/µl - Human. Other species not tested. - Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. - Rabbit)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '271',
'name' => 'C15210011',
'product_id' => '2882',
'modified' => '2020-02-20 17:58:04',
'created' => '2020-02-20 17:58:04'
),
'Master' => array(
'id' => '2882',
'antibody_id' => '500',
'name' => 'H3.3 Antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant <strong>H3.3</strong>, using a KLH-conjugated synthetic peptide.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chip.jpg" alt="H3.3 Antibody ChIP Grade" caption="false" width="278" height="212" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3.3 (cat. No. C15210011) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive MYOD1 and TSH2B genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-a.jpg" alt="H3.3 Antibody ChIP-seq Grade" caption="false" width="893" height="106" /></center><br /> B.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-b.jpg" alt="H3.3 Antibody for ChIP-seq " caption="false" width="893" height="274" /></center><br /> C.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-c.jpg" alt="H3.3 Antibody for ChIP-seq assay" caption="false" width="893" height="218" /></center><br /> D.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-d.jpg" alt="H3.3 Antibody validated in ChIP-seq" caption="false" width="893" height="176" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2.ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP was performed with 1 µg of the Diagenode antibody against H3.3 (cat. No. C15210011) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3.5 Mb region of human chromosome 1 (figure 2A and B) and in two genomic regions surrounding the CCT5 and EIF2S3 positive control genes (figure 2C and D). </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-wb.jpg" alt="H3.3 Antibody validated in Western Blot" caption="false" width="201" height="260" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H3.3</strong><br /> Western blot was performed on 1 µg of recombinant histone H3.1 (lane 2) and on 1 µg of recombinant histone H3.3 (lane 1) using the Diagenode monoclonal antibody against H3.3 (cat. No. C15210011) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the socalled "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.</p>',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15210011',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000526',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '435',
'price_USD' => '440',
'price_GBP' => '395',
'price_JPY' => '68145',
'price_CNY' => '',
'price_AUD' => '1100',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'March 16, 2017',
'slug' => 'h3-3-monoclonal-antibody',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:36',
'created' => '2017-04-06 12:28:50'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '42',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-seq (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-seq-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications',
'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode',
'modified' => '2016-01-20 11:06:19',
'created' => '2015-10-20 11:44:45',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '127',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-grade antibodies',
'description' => '<div class="row">
<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode',
'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
'modified' => '2024-11-19 17:27:07',
'created' => '2017-02-14 11:16:04',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '17',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-seq grade antibodies',
'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode’s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-seq-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)',
'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:22',
'created' => '2015-02-16 02:24:01',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 2 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 3 => array(
'id' => '102',
'position' => '1',
'parent_id' => '4',
'name' => 'Sample size antibodies',
'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => true,
'is_antibody' => true,
'slug' => 'sample-size-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
'meta_title' => 'Sample-size Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:05',
'created' => '2015-10-27 12:13:34',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 4 => array(
'id' => '111',
'position' => '40',
'parent_id' => '4',
'name' => 'Histone antibodies',
'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p>
<p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p>
<p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>”, analogous to the genetic code.</p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p>
<p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p>
<p>Diagenode’s collection includes antibodies recognizing:</p>
<ul>
<li><strong>Histone H1 variants</strong></li>
<li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li>
<li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li>
<li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li>
<li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li>
</ul>
<p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p>
<p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> Expert technical support</span></li>
<li><span style="font-weight: 400;"> Sample sizes available</span></li>
<li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li>
</ul>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'histone-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Histone, antibody, histone h1, histone h2, histone h3, histone h4',
'meta_description' => 'Polyclonal and Monoclonal Antibodies against Histones and their modifications validated for many applications, including Chromatin Immunoprecipitation (ChIP) and ChIP-Sequencing (ChIP-seq)',
'meta_title' => 'Histone and Modified Histone Antibodies | Diagenode',
'modified' => '2020-09-17 13:34:56',
'created' => '2016-04-01 16:01:32',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '953',
'name' => 'H3.3 monoclonal antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant H3.3, using a KLH-conjugated synthetic peptide.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/h3-3-monoclonal-antibody.pdf',
'slug' => 'h3-3-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-05-04 16:28:43',
'created' => '2017-05-04 16:28:43',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1783',
'name' => 'product/antibodies/chipseq-grade-ab-icon.png',
'alt' => 'ChIP-seq Grade',
'modified' => '2020-11-27 07:04:40',
'created' => '2018-03-15 15:54:09',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4174',
'name' => 'Dynamic association of the H3K64 trimethylation mark with genes encodingexported proteins in Plasmodium falciparum.',
'authors' => 'Jabeena, C A et al.',
'description' => '<p>Epigenetic modifications have emerged as critical regulators of virulence genes and stage-specific gene expression in Plasmodium falciparum. However, the specific roles of histone core epigenetic modifications in regulating the stage-specific gene expression are not well understood. In this study, we report an unconventional trimethylation at lysine 64 on histone 3 (H3K64me3) and characterize its functional relevance in P. falciparum. We show that PfSET4 and PfSET5 proteins of P. falciparum methylate H3K64 and that they prefer the nucleosome as a substrate over free histone 3 proteins. Structural analysis of PfSET5 revealed that it interacts with the nucleosome as a dimer. The H3K64me3 mark is dynamic, being enriched in the ring and trophozoite stages and drastically reduced in schizont stages. Stage-specific global ChIP-sequencing analysis of the H3K64me3 mark revealed the selective enrichment of this methyl mark on the genes of exported family proteins in the ring and trophozoite stages, and a significant reduction of the same in the schizont stages. Collectively, our data identify a novel epigenetic mark that are associated with the subset of genes encoding for exported proteins which may regulate their expression in different stages of P. falciparum.</p>',
'date' => '2021-04-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33839154',
'doi' => '10.1016/j.jbc.2021.100614',
'modified' => '2021-12-21 16:07:05',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '2998',
'name' => 'H3.3 Antibody SDS GB en',
'language' => 'en',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-GB-en-GHS_1_0.pdf',
'countries' => 'GB',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '3000',
'name' => 'H3.3 Antibody SDS US en',
'language' => 'en',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-US-en-GHS_1_0.pdf',
'countries' => 'US',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '2995',
'name' => 'H3.3 Antibody SDS DE de',
'language' => 'de',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-DE-de-GHS_1_0.pdf',
'countries' => 'DE',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '2999',
'name' => 'H3.3 Antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-JP-ja-GHS_1_0.pdf',
'countries' => 'JP',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '2994',
'name' => 'H3.3 Antibody SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '2993',
'name' => 'H3.3 Antibody SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-BE-fr-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '2997',
'name' => 'H3.3 Antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-FR-fr-GHS_1_0.pdf',
'countries' => 'FR',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '2996',
'name' => 'H3.3 Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/h3-3-monoclonal-antibody'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '2882',
'antibody_id' => '500',
'name' => 'H3.3 Antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant <strong>H3.3</strong>, using a KLH-conjugated synthetic peptide.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chip.jpg" alt="H3.3 Antibody ChIP Grade" caption="false" width="278" height="212" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3.3 (cat. No. C15210011) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive MYOD1 and TSH2B genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-a.jpg" alt="H3.3 Antibody ChIP-seq Grade" caption="false" width="893" height="106" /></center><br /> B.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-b.jpg" alt="H3.3 Antibody for ChIP-seq " caption="false" width="893" height="274" /></center><br /> C.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-c.jpg" alt="H3.3 Antibody for ChIP-seq assay" caption="false" width="893" height="218" /></center><br /> D.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-d.jpg" alt="H3.3 Antibody validated in ChIP-seq" caption="false" width="893" height="176" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2.ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP was performed with 1 µg of the Diagenode antibody against H3.3 (cat. No. C15210011) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3.5 Mb region of human chromosome 1 (figure 2A and B) and in two genomic regions surrounding the CCT5 and EIF2S3 positive control genes (figure 2C and D). </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-wb.jpg" alt="H3.3 Antibody validated in Western Blot" caption="false" width="201" height="260" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H3.3</strong><br /> Western blot was performed on 1 µg of recombinant histone H3.1 (lane 2) and on 1 µg of recombinant histone H3.3 (lane 1) using the Diagenode monoclonal antibody against H3.3 (cat. No. C15210011) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the socalled "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.</p>',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15210011',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000526',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '435',
'price_USD' => '440',
'price_GBP' => '395',
'price_JPY' => '68145',
'price_CNY' => '',
'price_AUD' => '1100',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'March 16, 2017',
'slug' => 'h3-3-monoclonal-antibody',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:36',
'created' => '2017-04-06 12:28:50'
)
)
$pro = array(
'id' => '3055',
'antibody_id' => '500',
'name' => 'H3.3 Antibody (sample size)',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15210011-10',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000582',
'type' => 'RFR',
'search_order' => '',
'price_EUR' => '110',
'price_USD' => '120',
'price_GBP' => '105',
'price_JPY' => '17230',
'price_CNY' => '0',
'price_AUD' => '300',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'h3-3-monoclonal-antibody-10-ug',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode, sample size',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:49',
'created' => '2020-02-20 17:57:35',
'ProductsGroup' => array(
'id' => '306',
'product_id' => '3055',
'group_id' => '271'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2882',
'antibody_id' => '500',
'name' => 'H3.3 Antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant <strong>H3.3</strong>, using a KLH-conjugated synthetic peptide.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chip.jpg" alt="H3.3 Antibody ChIP Grade" caption="false" width="278" height="212" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3.3 (cat. No. C15210011) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive MYOD1 and TSH2B genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-a.jpg" alt="H3.3 Antibody ChIP-seq Grade" caption="false" width="893" height="106" /></center><br /> B.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-b.jpg" alt="H3.3 Antibody for ChIP-seq " caption="false" width="893" height="274" /></center><br /> C.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-c.jpg" alt="H3.3 Antibody for ChIP-seq assay" caption="false" width="893" height="218" /></center><br /> D.<br /><center><img src="https://www.diagenode.com/img/product/antibodies/c15210011-chipseq-d.jpg" alt="H3.3 Antibody validated in ChIP-seq" caption="false" width="893" height="176" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2.ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3.3</strong><br /> ChIP was performed with 1 µg of the Diagenode antibody against H3.3 (cat. No. C15210011) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3.5 Mb region of human chromosome 1 (figure 2A and B) and in two genomic regions surrounding the CCT5 and EIF2S3 positive control genes (figure 2C and D). </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15210011-wb.jpg" alt="H3.3 Antibody validated in Western Blot" caption="false" width="201" height="260" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H3.3</strong><br /> Western blot was performed on 1 µg of recombinant histone H3.1 (lane 2) and on 1 µg of recombinant histone H3.3 (lane 1) using the Diagenode monoclonal antibody against H3.3 (cat. No. C15210011) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the socalled "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.</p>',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15210011',
'old_catalog_number' => '',
'sf_code' => 'C15210011-D001-000526',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '435',
'price_USD' => '440',
'price_GBP' => '395',
'price_JPY' => '68145',
'price_CNY' => '',
'price_AUD' => '1100',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'March 16, 2017',
'slug' => 'h3-3-monoclonal-antibody',
'meta_title' => 'H3.3 Antibody - ChIP-seq Grade (C15210011) | Diagenode',
'meta_keywords' => 'H3.3 monoclonal antibody,Histone,Diagenode',
'meta_description' => 'Histone variant H3.3 Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
'modified' => '2021-12-23 11:57:36',
'created' => '2017-04-06 12:28:50'
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
'id' => '5123',
'product_id' => '3055',
'application_id' => '43'
)
)
$slugs = array(
(int) 0 => 'chip-qpcr-antibodies'
)
$applications = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'locale' => 'zho'
)
$description = ''
$name = 'ChIP-qPCR (ab)'
$document = array(
'id' => '953',
'name' => 'H3.3 monoclonal antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against histone variant H3.3, using a KLH-conjugated synthetic peptide.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/h3-3-monoclonal-antibody.pdf',
'slug' => 'h3-3-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-05-04 16:28:43',
'created' => '2017-05-04 16:28:43',
'ProductsDocument' => array(
'id' => '2860',
'product_id' => '3055',
'document_id' => '953'
)
)
$sds = array(
'id' => '2996',
'name' => 'H3.3 Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/H3_3/SDS-C15210011-H3_3_Antibody-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2023-01-10 13:50:00',
'created' => '2023-01-10 13:50:00',
'ProductsSafetySheet' => array(
'id' => '4912',
'product_id' => '3055',
'safety_sheet_id' => '2996'
)
)
$publication = array(
'id' => '4174',
'name' => 'Dynamic association of the H3K64 trimethylation mark with genes encodingexported proteins in Plasmodium falciparum.',
'authors' => 'Jabeena, C A et al.',
'description' => '<p>Epigenetic modifications have emerged as critical regulators of virulence genes and stage-specific gene expression in Plasmodium falciparum. However, the specific roles of histone core epigenetic modifications in regulating the stage-specific gene expression are not well understood. In this study, we report an unconventional trimethylation at lysine 64 on histone 3 (H3K64me3) and characterize its functional relevance in P. falciparum. We show that PfSET4 and PfSET5 proteins of P. falciparum methylate H3K64 and that they prefer the nucleosome as a substrate over free histone 3 proteins. Structural analysis of PfSET5 revealed that it interacts with the nucleosome as a dimer. The H3K64me3 mark is dynamic, being enriched in the ring and trophozoite stages and drastically reduced in schizont stages. Stage-specific global ChIP-sequencing analysis of the H3K64me3 mark revealed the selective enrichment of this methyl mark on the genes of exported family proteins in the ring and trophozoite stages, and a significant reduction of the same in the schizont stages. Collectively, our data identify a novel epigenetic mark that are associated with the subset of genes encoding for exported proteins which may regulate their expression in different stages of P. falciparum.</p>',
'date' => '2021-04-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33839154',
'doi' => '10.1016/j.jbc.2021.100614',
'modified' => '2021-12-21 16:07:05',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
'id' => '5368',
'product_id' => '3055',
'publication_id' => '4174'
)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/33839154" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
×
