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H3K36me3 monoclonal antibody

Histone-Deacetylase-polyclonal-antibody-diagenode

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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C15210013

100 µg

$440.00
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Monoclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 36 (H3K36me3), using a KLH-conjugated synthetic peptide.

Lot	001
Concentration	1 μg/μl
Species reactivity	Human, wide range expected.
Type	Monoclonal ChIP-seq grade
Purity	Affinity purified polyclonal antibody.
Host	Rabbit
Storage Conditions	Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage Buffer	PBS containing 50% glycerol, 1% BSA and 0.09% azide.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ChIP/ChIP-seq ^*	0.5 - 1 µg per IP	Fig 1, 2
Dot Blotting	1:2,000	Fig 3
Western Blotting	1:500	Fig 4

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 µg per IP.

Validation data

Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K36me3
ChIP was performed with the Diagenode antibody against H3K36me3 (cat. No. C15210013) on sheared chromatin from 500,000 HeLaS3 cells using the “iDeal ChIP-seq” kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the CCT5 coding region, used as positive control, and for a region upstream the ACTB promoter, used as negative control. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

A.
B.
C.
D.

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K36me3
ChIP was performed on sheared chromatin from 500,000 HeLaS3 cells using 1 µg of the Diagenode antibody against H3K36me3 (cat. No. C15210013) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the H3K36me3 signal along the complete sequence and 1.5 Mb region of human chromosome 1 (figure 2A and B), in a genomic region on chromosome X surrounding the EIF2S3 gene (figure 2C) and in a genomic region surrounding the CCT5 positive control (figure 2D).

Figure 3. Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K36me3
To test the cross reactivity of the Diagenode antibody against H3K36me3 (cat. No. C15210013), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K36. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest.

Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K36me3
Western blot was performed on recombinant histone H3 (1 µg) and on histone extracts from HeLa cells (40 µg) using the Diagenode antibody against H3K36me3 (cat. No. C15210013). The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.

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			'description' => '<p>Monoclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 36 (H3K36me3), using a KLH-conjugated synthetic peptide.</p>',
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-chip.jpg" alt="H3K36me3 Antibody ChIP Grade" /></center></div>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K36me3</strong><br />ChIP was performed with the Diagenode antibody against H3K36me3 (cat. No. C15210013) on sheared chromatin from 500,000 HeLaS3 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 &micro;g per ChIP experiment was analysed. IgG (1 &micro;g/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the CCT5 coding region, used as positive control, and for a region upstream the ACTB promoter, used as negative control. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2A-ChIP-seq.jpg" alt="H3K36me3 Antibody ChIP-seq Grade" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2B-ChIP-seq.jpg" alt="H3K36me3 Antibody for ChIP-seq" /></center><br /> C.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2C-ChIP-seq.jpg" alt="H3K36me3 Antibody for ChIP-seq assay" /></center><br /> D.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2D-ChIP-seq.jpg" alt="H3K36me3 Antibody validated in ChIP-seq" /></center></div>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K36me3</strong><br />ChIP was performed on sheared chromatin from 500,000 HeLaS3 cells using 1 &micro;g of the Diagenode antibody against H3K36me3 (cat. No. C15210013) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the H3K36me3 signal along the complete sequence and 1.5 Mb region of human chromosome 1 (figure 2A and B), in a genomic region on chromosome X surrounding the EIF2S3 gene (figure 2C) and in a genomic region surrounding the CCT5 positive control (figure 2D).</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-db.jpg" alt="H3K36me3 Antibody Dot Blot Validation" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 3. Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K36me3</strong><br />To test the cross reactivity of the Diagenode antibody against H3K36me3 (cat. No. C15210013), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K36. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest.</p>
</div>
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<div class="row">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-wb.jpg" alt="H3K36me3 Antibody validated in Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K36me3</strong><br />Western blot was performed on recombinant histone H3 (1 &micro;g) and on histone extracts from HeLa cells (40 &micro;g) using the Diagenode antibody against H3K36me3 (cat. No. C15210013). The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.</p>
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<td>ChIP/ChIP-seq&nbsp;<sup>*</sup></td>
<td>0.5 - 1 &micro;g per IP</td>
<td>Fig 1, 2</td>
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<tr>
<td>Dot Blotting</td>
<td>1:2,000</td>
<td>Fig&nbsp;3</td>
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<td>Western Blotting</td>
<td>1:500</td>
<td>Fig&nbsp;4</td>
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<p></p>
<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 &micro;g per IP.</small></p>',
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		'name' => 'H3K36me3 monoclonal antibody',
		'description' => '<p>Monoclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 36 (H3K36me3), using a KLH-conjugated synthetic peptide.</p>',
		'label1' => 'Validation data',
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-chip.jpg" alt="H3K36me3 Antibody ChIP Grade" /></center></div>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K36me3</strong><br />ChIP was performed with the Diagenode antibody against H3K36me3 (cat. No. C15210013) on sheared chromatin from 500,000 HeLaS3 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 &micro;g per ChIP experiment was analysed. IgG (1 &micro;g/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the CCT5 coding region, used as positive control, and for a region upstream the ACTB promoter, used as negative control. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2A-ChIP-seq.jpg" alt="H3K36me3 Antibody ChIP-seq Grade" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2B-ChIP-seq.jpg" alt="H3K36me3 Antibody for ChIP-seq" /></center><br /> C.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2C-ChIP-seq.jpg" alt="H3K36me3 Antibody for ChIP-seq assay" /></center><br /> D.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2D-ChIP-seq.jpg" alt="H3K36me3 Antibody validated in ChIP-seq" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K36me3</strong><br />ChIP was performed on sheared chromatin from 500,000 HeLaS3 cells using 1 &micro;g of the Diagenode antibody against H3K36me3 (cat. No. C15210013) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the H3K36me3 signal along the complete sequence and 1.5 Mb region of human chromosome 1 (figure 2A and B), in a genomic region on chromosome X surrounding the EIF2S3 gene (figure 2C) and in a genomic region surrounding the CCT5 positive control (figure 2D).</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-db.jpg" alt="H3K36me3 Antibody Dot Blot Validation" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 3. Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K36me3</strong><br />To test the cross reactivity of the Diagenode antibody against H3K36me3 (cat. No. C15210013), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K36. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest.</p>
</div>
</div>
<div class="row">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-wb.jpg" alt="H3K36me3 Antibody validated in Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K36me3</strong><br />Western blot was performed on recombinant histone H3 (1 &micro;g) and on histone extracts from HeLa cells (40 &micro;g) using the Diagenode antibody against H3K36me3 (cat. No. C15210013). The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.</p>
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		'name' => 'H3K36me3 Antibody - ChIP-seq Grade',
		'description' => 'Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K36 is associated with active genes.',
		'clonality' => '',
		'isotype' => '',
		'lot' => '001',
		'concentration' => '1 μg/μl',
		'reactivity' => 'Human, wide range expected.',
		'type' => 'Monoclonal <strong>ChIP-seq grade</strong>',
		'purity' => 'Affinity purified polyclonal antibody.',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq&nbsp;<sup>*</sup></td>
<td>0.5 - 1 &micro;g per IP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Dot Blotting</td>
<td>1:2,000</td>
<td>Fig&nbsp;3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:500</td>
<td>Fig&nbsp;4</td>
</tr>
</tbody>
</table>
<p></p>
<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 &micro;g per IP.</small></p>',
		'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
		'storage_buffer' => 'PBS containing 50% glycerol, 1% BSA and 0.09% azide.',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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		'slug' => 'h3k36me3-monoclonal-antibody',
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		'modified' => '2020-09-18 15:54:21',
		'created' => '2020-05-26 11:29:40',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications',
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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			'name' => 'ChIP-grade antibodies',
			'description' => '<div class="row">
<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
			'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
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			'name' => 'Histone antibodies',
			'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p>
<p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome.&nbsp; They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include&nbsp; methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation.&nbsp; The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p>
<p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>&rdquo;, analogous to the genetic code.</p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p>
<p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our&nbsp; antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p>
<p>Diagenode&rsquo;s collection includes antibodies recognizing:</p>
<ul>
<li><strong>Histone H1 variants</strong></li>
<li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li>
<li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li>
<li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li>
<li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li>
</ul>
<p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p>
<p><span style="font-weight: 400;"> Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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View::render() - CORE/Cake/View/View.php, line 473
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ReflectionMethod::invokeArgs() - [internal], line ??
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			'name' => 'H3K36me3 monoclonal antibody',
			'description' => '<p>Monoclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 36 (H3K36me3), using a KLH-conjugated synthetic peptide.</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-chip.jpg" alt="H3K36me3 Antibody ChIP Grade" /></center></div>
<div class="small-6 columns">
<p><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K36me3</strong><br />ChIP was performed with the Diagenode antibody against H3K36me3 (cat. No. C15210013) on sheared chromatin from 500,000 HeLaS3 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 &micro;g per ChIP experiment was analysed. IgG (1 &micro;g/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the CCT5 coding region, used as positive control, and for a region upstream the ACTB promoter, used as negative control. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2A-ChIP-seq.jpg" alt="H3K36me3 Antibody ChIP-seq Grade" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2B-ChIP-seq.jpg" alt="H3K36me3 Antibody for ChIP-seq" /></center><br /> C.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2C-ChIP-seq.jpg" alt="H3K36me3 Antibody for ChIP-seq assay" /></center><br /> D.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2D-ChIP-seq.jpg" alt="H3K36me3 Antibody validated in ChIP-seq" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K36me3</strong><br />ChIP was performed on sheared chromatin from 500,000 HeLaS3 cells using 1 &micro;g of the Diagenode antibody against H3K36me3 (cat. No. C15210013) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the H3K36me3 signal along the complete sequence and 1.5 Mb region of human chromosome 1 (figure 2A and B), in a genomic region on chromosome X surrounding the EIF2S3 gene (figure 2C) and in a genomic region surrounding the CCT5 positive control (figure 2D).</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-db.jpg" alt="H3K36me3 Antibody Dot Blot Validation" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 3. Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K36me3</strong><br />To test the cross reactivity of the Diagenode antibody against H3K36me3 (cat. No. C15210013), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K36. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-wb.jpg" alt="H3K36me3 Antibody validated in Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K36me3</strong><br />Western blot was performed on recombinant histone H3 (1 &micro;g) and on histone extracts from HeLa cells (40 &micro;g) using the Diagenode antibody against H3K36me3 (cat. No. C15210013). The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.</p>
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			'concentration' => '1 μg/μl',
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<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<td>ChIP/ChIP-seq&nbsp;<sup>*</sup></td>
<td>0.5 - 1 &micro;g per IP</td>
<td>Fig 1, 2</td>
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<tr>
<td>Dot Blotting</td>
<td>1:2,000</td>
<td>Fig&nbsp;3</td>
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<tr>
<td>Western Blotting</td>
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<td>Fig&nbsp;4</td>
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<p></p>
<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 &micro;g per IP.</small></p>',
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<p><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K36me3</strong><br />ChIP was performed with the Diagenode antibody against H3K36me3 (cat. No. C15210013) on sheared chromatin from 500,000 HeLaS3 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 &micro;g per ChIP experiment was analysed. IgG (1 &micro;g/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the CCT5 coding region, used as positive control, and for a region upstream the ACTB promoter, used as negative control. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2A-ChIP-seq.jpg" alt="H3K36me3 Antibody ChIP-seq Grade" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2B-ChIP-seq.jpg" alt="H3K36me3 Antibody for ChIP-seq" /></center><br /> C.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2C-ChIP-seq.jpg" alt="H3K36me3 Antibody for ChIP-seq assay" /></center><br /> D.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2D-ChIP-seq.jpg" alt="H3K36me3 Antibody validated in ChIP-seq" /></center></div>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K36me3</strong><br />ChIP was performed on sheared chromatin from 500,000 HeLaS3 cells using 1 &micro;g of the Diagenode antibody against H3K36me3 (cat. No. C15210013) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the H3K36me3 signal along the complete sequence and 1.5 Mb region of human chromosome 1 (figure 2A and B), in a genomic region on chromosome X surrounding the EIF2S3 gene (figure 2C) and in a genomic region surrounding the CCT5 positive control (figure 2D).</p>
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<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-db.jpg" alt="H3K36me3 Antibody Dot Blot Validation" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 3. Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K36me3</strong><br />To test the cross reactivity of the Diagenode antibody against H3K36me3 (cat. No. C15210013), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K36. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest.</p>
</div>
</div>
<div class="row">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-wb.jpg" alt="H3K36me3 Antibody validated in Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K36me3</strong><br />Western blot was performed on recombinant histone H3 (1 &micro;g) and on histone extracts from HeLa cells (40 &micro;g) using the Diagenode antibody against H3K36me3 (cat. No. C15210013). The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.</p>
</div>
</div>',
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		'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K36 is associated with active genes.</p>',
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		'format' => '100 µg',
		'catalog_number' => 'C15210013',
		'old_catalog_number' => '',
		'sf_code' => 'C15210013-D001-000526',
		'type' => 'RFR',
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		'price_EUR' => '435',
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		'slug' => 'h3k36me3-monoclonal-antibody-100-ug',
		'meta_title' => 'H3K36me3 monoclonal antibody',
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		'meta_description' => 'H3K36me3 monoclonal antibody',
		'modified' => '2021-12-23 11:29:08',
		'created' => '2020-05-26 11:44:03',
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	'Antibody' => array(
		'host' => '*****',
		'id' => '558',
		'name' => 'H3K36me3 Antibody - ChIP-seq Grade',
		'description' => 'Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K36 is associated with active genes.',
		'clonality' => '',
		'isotype' => '',
		'lot' => '001',
		'concentration' => '1 μg/μl',
		'reactivity' => 'Human, wide range expected.',
		'type' => 'Monoclonal <strong>ChIP-seq grade</strong>',
		'purity' => 'Affinity purified polyclonal antibody.',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<tbody>
<tr>
<td>ChIP/ChIP-seq&nbsp;<sup>*</sup></td>
<td>0.5 - 1 &micro;g per IP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Dot Blotting</td>
<td>1:2,000</td>
<td>Fig&nbsp;3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:500</td>
<td>Fig&nbsp;4</td>
</tr>
</tbody>
</table>
<p></p>
<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 &micro;g per IP.</small></p>',
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		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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		'slug' => 'h3k36me3-monoclonal-antibody',
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		'modified' => '2020-09-18 15:54:21',
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		'select_label' => '558 - H3K36me3 Antibody - ChIP-seq Grade (001 - 1 μg/μl - Human, wide range expected. - Affinity purified polyclonal antibody. - Rabbit)'
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications',
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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			'name' => 'ChIP-grade antibodies',
			'description' => '<div class="row">
<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
			'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
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			'name' => 'Histone antibodies',
			'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p>
<p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome.&nbsp; They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include&nbsp; methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation.&nbsp; The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p>
<p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>&rdquo;, analogous to the genetic code.</p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p>
<p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our&nbsp; antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p>
<p>Diagenode&rsquo;s collection includes antibodies recognizing:</p>
<ul>
<li><strong>Histone H1 variants</strong></li>
<li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li>
<li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li>
<li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li>
<li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li>
</ul>
<p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p>
<p><span style="font-weight: 400;"> Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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			'name' => 'H3K36me3 monoclonal antibody',
			'description' => '<p>Monoclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 36 (H3K36me3), using a KLH-conjugated synthetic peptide.</p>',
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-chip.jpg" alt="H3K36me3 Antibody ChIP Grade" /></center></div>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K36me3</strong><br />ChIP was performed with the Diagenode antibody against H3K36me3 (cat. No. C15210013) on sheared chromatin from 500,000 HeLaS3 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 &micro;g per ChIP experiment was analysed. IgG (1 &micro;g/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the CCT5 coding region, used as positive control, and for a region upstream the ACTB promoter, used as negative control. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2A-ChIP-seq.jpg" alt="H3K36me3 Antibody ChIP-seq Grade" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2B-ChIP-seq.jpg" alt="H3K36me3 Antibody for ChIP-seq" /></center><br /> C.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2C-ChIP-seq.jpg" alt="H3K36me3 Antibody for ChIP-seq assay" /></center><br /> D.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2D-ChIP-seq.jpg" alt="H3K36me3 Antibody validated in ChIP-seq" /></center></div>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K36me3</strong><br />ChIP was performed on sheared chromatin from 500,000 HeLaS3 cells using 1 &micro;g of the Diagenode antibody against H3K36me3 (cat. No. C15210013) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the H3K36me3 signal along the complete sequence and 1.5 Mb region of human chromosome 1 (figure 2A and B), in a genomic region on chromosome X surrounding the EIF2S3 gene (figure 2C) and in a genomic region surrounding the CCT5 positive control (figure 2D).</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-db.jpg" alt="H3K36me3 Antibody Dot Blot Validation" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 3. Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K36me3</strong><br />To test the cross reactivity of the Diagenode antibody against H3K36me3 (cat. No. C15210013), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K36. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K36me3</strong><br />Western blot was performed on recombinant histone H3 (1 &micro;g) and on histone extracts from HeLa cells (40 &micro;g) using the Diagenode antibody against H3K36me3 (cat. No. C15210013). The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.</p>
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<td>ChIP/ChIP-seq&nbsp;<sup>*</sup></td>
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<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 &micro;g per IP.</small></p>',
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-chip.jpg" alt="H3K36me3 Antibody ChIP Grade" /></center></div>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K36me3</strong><br />ChIP was performed with the Diagenode antibody against H3K36me3 (cat. No. C15210013) on sheared chromatin from 500,000 HeLaS3 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 &micro;g per ChIP experiment was analysed. IgG (1 &micro;g/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the CCT5 coding region, used as positive control, and for a region upstream the ACTB promoter, used as negative control. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2A-ChIP-seq.jpg" alt="H3K36me3 Antibody ChIP-seq Grade" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2B-ChIP-seq.jpg" alt="H3K36me3 Antibody for ChIP-seq" /></center><br /> C.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2C-ChIP-seq.jpg" alt="H3K36me3 Antibody for ChIP-seq assay" /></center><br /> D.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2D-ChIP-seq.jpg" alt="H3K36me3 Antibody validated in ChIP-seq" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K36me3</strong><br />ChIP was performed on sheared chromatin from 500,000 HeLaS3 cells using 1 &micro;g of the Diagenode antibody against H3K36me3 (cat. No. C15210013) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the H3K36me3 signal along the complete sequence and 1.5 Mb region of human chromosome 1 (figure 2A and B), in a genomic region on chromosome X surrounding the EIF2S3 gene (figure 2C) and in a genomic region surrounding the CCT5 positive control (figure 2D).</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-db.jpg" alt="H3K36me3 Antibody Dot Blot Validation" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 3. Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K36me3</strong><br />To test the cross reactivity of the Diagenode antibody against H3K36me3 (cat. No. C15210013), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K36. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest.</p>
</div>
</div>
<div class="row">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-wb.jpg" alt="H3K36me3 Antibody validated in Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K36me3</strong><br />Western blot was performed on recombinant histone H3 (1 &micro;g) and on histone extracts from HeLa cells (40 &micro;g) using the Diagenode antibody against H3K36me3 (cat. No. C15210013). The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.</p>
</div>
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		'label2' => '',
		'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K36 is associated with active genes.</p>',
		'label3' => '',
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		'format' => '100 µg',
		'catalog_number' => 'C15210013',
		'old_catalog_number' => '',
		'sf_code' => 'C15210013-D001-000526',
		'type' => 'RFR',
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		'price_EUR' => '435',
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		'slug' => 'h3k36me3-monoclonal-antibody-100-ug',
		'meta_title' => 'H3K36me3 monoclonal antibody',
		'meta_keywords' => '',
		'meta_description' => 'H3K36me3 monoclonal antibody',
		'modified' => '2021-12-23 11:29:08',
		'created' => '2020-05-26 11:44:03',
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	'Antibody' => array(
		'host' => '*****',
		'id' => '558',
		'name' => 'H3K36me3 Antibody - ChIP-seq Grade',
		'description' => 'Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K36 is associated with active genes.',
		'clonality' => '',
		'isotype' => '',
		'lot' => '001',
		'concentration' => '1 μg/μl',
		'reactivity' => 'Human, wide range expected.',
		'type' => 'Monoclonal <strong>ChIP-seq grade</strong>',
		'purity' => 'Affinity purified polyclonal antibody.',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq&nbsp;<sup>*</sup></td>
<td>0.5 - 1 &micro;g per IP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Dot Blotting</td>
<td>1:2,000</td>
<td>Fig&nbsp;3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:500</td>
<td>Fig&nbsp;4</td>
</tr>
</tbody>
</table>
<p></p>
<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 &micro;g per IP.</small></p>',
		'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
		'storage_buffer' => 'PBS containing 50% glycerol, 1% BSA and 0.09% azide.',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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		'slug' => 'h3k36me3-monoclonal-antibody',
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		'modified' => '2020-09-18 15:54:21',
		'created' => '2020-05-26 11:29:40',
		'select_label' => '558 - H3K36me3 Antibody - ChIP-seq Grade (001 - 1 μg/μl - Human, wide range expected. - Affinity purified polyclonal antibody. - Rabbit)'
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
			'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
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			'slug' => 'chip-seq-antibodies',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications',
			'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode',
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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			'name' => 'ChIP-grade antibodies',
			'description' => '<div class="row">
<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode',
			'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
			'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
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			'name' => 'ChIP-seq grade antibodies',
			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
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			'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)',
			'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
			'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
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		(int) 3 => array(
			'id' => '111',
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			'name' => 'Histone antibodies',
			'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p>
<p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome.&nbsp; They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include&nbsp; methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation.&nbsp; The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p>
<p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>&rdquo;, analogous to the genetic code.</p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p>
<p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our&nbsp; antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p>
<p>Diagenode&rsquo;s collection includes antibodies recognizing:</p>
<ul>
<li><strong>Histone H1 variants</strong></li>
<li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li>
<li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li>
<li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li>
<li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li>
</ul>
<p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p>
<p><span style="font-weight: 400;"> Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
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			'description' => '<p>Monoclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 36 (H3K36me3), using a KLH-conjugated synthetic peptide.</p>',
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-chip.jpg" alt="H3K36me3 Antibody ChIP Grade" /></center></div>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K36me3</strong><br />ChIP was performed with the Diagenode antibody against H3K36me3 (cat. No. C15210013) on sheared chromatin from 500,000 HeLaS3 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 &micro;g per ChIP experiment was analysed. IgG (1 &micro;g/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the CCT5 coding region, used as positive control, and for a region upstream the ACTB promoter, used as negative control. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2A-ChIP-seq.jpg" alt="H3K36me3 Antibody ChIP-seq Grade" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2B-ChIP-seq.jpg" alt="H3K36me3 Antibody for ChIP-seq" /></center><br /> C.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2C-ChIP-seq.jpg" alt="H3K36me3 Antibody for ChIP-seq assay" /></center><br /> D.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2D-ChIP-seq.jpg" alt="H3K36me3 Antibody validated in ChIP-seq" /></center></div>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K36me3</strong><br />ChIP was performed on sheared chromatin from 500,000 HeLaS3 cells using 1 &micro;g of the Diagenode antibody against H3K36me3 (cat. No. C15210013) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the H3K36me3 signal along the complete sequence and 1.5 Mb region of human chromosome 1 (figure 2A and B), in a genomic region on chromosome X surrounding the EIF2S3 gene (figure 2C) and in a genomic region surrounding the CCT5 positive control (figure 2D).</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-db.jpg" alt="H3K36me3 Antibody Dot Blot Validation" /></center></div>
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<p><strong>Figure 3. Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K36me3</strong><br />To test the cross reactivity of the Diagenode antibody against H3K36me3 (cat. No. C15210013), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K36. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K36me3</strong><br />Western blot was performed on recombinant histone H3 (1 &micro;g) and on histone extracts from HeLa cells (40 &micro;g) using the Diagenode antibody against H3K36me3 (cat. No. C15210013). The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.</p>
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<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 &micro;g per IP.</small></p>',
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		'id' => '3075',
		'antibody_id' => '558',
		'name' => 'H3K36me3 monoclonal antibody',
		'description' => '<p>Monoclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 36 (H3K36me3), using a KLH-conjugated synthetic peptide.</p>',
		'label1' => 'Validation data',
		'info1' => '<div class="row">
<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-chip.jpg" alt="H3K36me3 Antibody ChIP Grade" /></center></div>
<div class="small-6 columns">
<p><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K36me3</strong><br />ChIP was performed with the Diagenode antibody against H3K36me3 (cat. No. C15210013) on sheared chromatin from 500,000 HeLaS3 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 &micro;g per ChIP experiment was analysed. IgG (1 &micro;g/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the CCT5 coding region, used as positive control, and for a region upstream the ACTB promoter, used as negative control. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2A-ChIP-seq.jpg" alt="H3K36me3 Antibody ChIP-seq Grade" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2B-ChIP-seq.jpg" alt="H3K36me3 Antibody for ChIP-seq" /></center><br /> C.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2C-ChIP-seq.jpg" alt="H3K36me3 Antibody for ChIP-seq assay" /></center><br /> D.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210013-fig2D-ChIP-seq.jpg" alt="H3K36me3 Antibody validated in ChIP-seq" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K36me3</strong><br />ChIP was performed on sheared chromatin from 500,000 HeLaS3 cells using 1 &micro;g of the Diagenode antibody against H3K36me3 (cat. No. C15210013) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the H3K36me3 signal along the complete sequence and 1.5 Mb region of human chromosome 1 (figure 2A and B), in a genomic region on chromosome X surrounding the EIF2S3 gene (figure 2C) and in a genomic region surrounding the CCT5 positive control (figure 2D).</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-db.jpg" alt="H3K36me3 Antibody Dot Blot Validation" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 3. Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K36me3</strong><br />To test the cross reactivity of the Diagenode antibody against H3K36me3 (cat. No. C15210013), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K36. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210014-wb.jpg" alt="H3K36me3 Antibody validated in Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K36me3</strong><br />Western blot was performed on recombinant histone H3 (1 &micro;g) and on histone extracts from HeLa cells (40 &micro;g) using the Diagenode antibody against H3K36me3 (cat. No. C15210013). The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.</p>
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		'description' => 'Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K36 is associated with active genes.',
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		'isotype' => '',
		'lot' => '001',
		'concentration' => '1 μg/μl',
		'reactivity' => 'Human, wide range expected.',
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<td>ChIP/ChIP-seq&nbsp;<sup>*</sup></td>
<td>0.5 - 1 &micro;g per IP</td>
<td>Fig 1, 2</td>
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<td>Dot Blotting</td>
<td>1:2,000</td>
<td>Fig&nbsp;3</td>
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<td>Fig&nbsp;4</td>
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<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 &micro;g per IP.</small></p>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'name' => 'Histone antibodies',
			'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p>
<p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome.&nbsp; They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include&nbsp; methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation.&nbsp; The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p>
<p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>&rdquo;, analogous to the genetic code.</p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p>
<p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our&nbsp; antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p>
<p>Diagenode&rsquo;s collection includes antibodies recognizing:</p>
<ul>
<li><strong>Histone H1 variants</strong></li>
<li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li>
<li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li>
<li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li>
<li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li>
</ul>
<p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p>
<p><span style="font-weight: 400;"> Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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Datasheet H3K36me3 monoclonal antibody DATASHEET Monoclonal antibody raised in rabbit against the region of histone H3 containing the trimethylate...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

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