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Polyclonal antibody raised in rabbit against the region of histone H3 containing the phosphorylated threonine 45 (H3T45p), using a KLH-conjugated synthetic peptide.
Lot
A2280P
Concentration
1.5 µg/µl
Species reactivity
Human
Type
Polyclonal
Purity
Affinity purified
Host
Rabbit
Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against H3T45p (cat. No. 15410321) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:350,000.
Figure 2. Cross reactivity tests using the Diagenode antibody directed against H3T45p To test the cross reactivity of the Diagenode antibody against H3T45p (cat. No. 15410321), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3T45. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 2 shows a high specificity of the antibody for the modification of interest.
