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<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
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<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
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<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
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<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
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<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
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<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
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<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
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<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
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<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
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<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
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<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
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<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
</div>
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<th>References</th>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 µg/ChIP</td>
<td>Fig 1, 2</td>
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<tr>
<td>ELISA</td>
<td>1:10,000</td>
<td>Fig 3</td>
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<td>Western Blotting</td>
<td>Not recommended</td>
<td></td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p>',
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<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
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<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
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<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
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<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
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<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
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<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
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'description' => '<p><strong>Other names:</strong> HD3, RPD3, RPD3-2, SMAP45</p>
<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
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<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
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<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
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'info2' => '<p>HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.</p>',
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