Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'cn',
'meta_keywords' => '',
'meta_description' => '',
'meta_title' => '',
'product' => array(
'Product' => array(
'id' => '3182',
'antibody_id' => '643',
'name' => 'HDAC3 Antibody - ChIP-seq Grade (sample size)',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15410364-10',
'old_catalog_number' => '',
'sf_code' => 'C15410364-D001-000582',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_title' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2024-01-17 19:48:49',
'created' => '2020-12-30 14:47:56',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '643',
'name' => 'HDAC3 Antibody - ChIP-seq Grade (sample size)',
'description' => 'HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.',
'clonality' => '',
'isotype' => '',
'lot' => 'A2633-0040',
'concentration' => '1.7 µg/µl',
'reactivity' => 'Human: positive. Other species: not tested.',
'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>',
'purity' => 'Affinity purified polyclonal antibody.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 µg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:10,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>Not recommended</td>
<td></td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => 'PBS containing 0.05% azide.',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-12-30 14:43:44',
'created' => '2020-12-30 14:43:44',
'select_label' => '643 - HDAC3 Antibody - ChIP-seq Grade (sample size) (A2633-0040 - 1.7 µg/µl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/hdac3-polyclonal-antibody-50-ug'
)
$language = 'cn'
$meta_keywords = ''
$meta_description = ''
$meta_title = ''
$product = array(
'Product' => array(
'id' => '3182',
'antibody_id' => '643',
'name' => 'HDAC3 Antibody - ChIP-seq Grade (sample size)',
'description' => '<p><strong>Other names:</strong> HD3, RPD3, RPD3-2, SMAP45</p>
<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.</p>',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15410364-10',
'old_catalog_number' => '',
'sf_code' => 'C15410364-D001-000582',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_title' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2024-01-17 19:48:49',
'created' => '2020-12-30 14:47:56',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '643',
'name' => 'HDAC3 Antibody - ChIP-seq Grade (sample size)',
'description' => 'HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.',
'clonality' => '',
'isotype' => '',
'lot' => 'A2633-0040',
'concentration' => '1.7 µg/µl',
'reactivity' => 'Human: positive. Other species: not tested.',
'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>',
'purity' => 'Affinity purified polyclonal antibody.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 µg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:10,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>Not recommended</td>
<td></td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => 'PBS containing 0.05% azide.',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-12-30 14:43:44',
'created' => '2020-12-30 14:43:44',
'select_label' => '643 - HDAC3 Antibody - ChIP-seq Grade (sample size) (A2633-0040 - 1.7 µg/µl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '345',
'name' => 'C15410364',
'product_id' => '3000',
'modified' => '2020-12-30 14:48:18',
'created' => '2020-12-30 14:48:18'
),
'Master' => array(
'id' => '3000',
'antibody_id' => '548',
'name' => 'HDAC3 Antibody',
'description' => '<p><strong>Other names:</strong> HD3, RPD3, RPD3-2, SMAP45</p>
<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.</p>',
'label3' => '',
'info3' => '',
'format' => '50 µg',
'catalog_number' => 'C15410364',
'old_catalog_number' => 'C15410361',
'sf_code' => 'C15410364-D001-000581',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '0',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'December 13, 2019',
'slug' => 'hdac3-polyclonal-antibody-50-ug',
'meta_title' => 'HDAC3 Antibody - ChIP-seq Grade (C15410364) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'HDAC3 (Histone deacetylase 3) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: HD3, RPD3, RPD3-2, SMAP45.',
'modified' => '2024-01-17 19:48:28',
'created' => '2018-10-24 11:56:07'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '20',
'position' => '10',
'parent_id' => '40',
'name' => 'ELISA',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
</div>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'elisa-antibodies',
'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody',
'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications',
'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:21:41',
'created' => '2014-07-08 08:13:28',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '42',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-seq (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-seq-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications',
'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode',
'modified' => '2016-01-20 11:06:19',
'created' => '2015-10-20 11:44:45',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '17',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-seq grade antibodies',
'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode’s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-seq-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)',
'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:22',
'created' => '2015-02-16 02:24:01',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '127',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-grade antibodies',
'description' => '<div class="row">
<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode',
'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
'modified' => '2024-11-19 17:27:07',
'created' => '2017-02-14 11:16:04',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 2 => array(
'id' => '21',
'position' => '20',
'parent_id' => '4',
'name' => 'Histone modifying enzymes',
'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chromatin-modifying-proteins-histone-transferase',
'cookies_tag_id' => null,
'meta_keywords' => 'monoclonal antibodies,policlonal antibodies,Histone modifying enzymes',
'meta_description' => 'Diagenode Offers Polyclonal and Monoclonal Antibodies Against Histone Modifying Enzymes like: Histone Deacetylases, Histone Demethylases, Histone Transferases.',
'meta_title' => 'Histone modifying enzymes - Antibodies | Diagenode',
'modified' => '2019-07-04 16:19:15',
'created' => '2014-06-26 13:18:41',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 3 => array(
'id' => '102',
'position' => '1',
'parent_id' => '4',
'name' => 'Sample size antibodies',
'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => true,
'is_antibody' => true,
'slug' => 'sample-size-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
'meta_title' => 'Sample-size Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:05',
'created' => '2015-10-27 12:13:34',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 4 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1036',
'name' => 'HDAC3 polyclonal antibody',
'description' => '<p>Polyclonal antibody raised in rabbit against human HDAC3 (Histone deacetylase 3), using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/hdac3-polyclonal-antibody-50-ug.pdf',
'slug' => 'hdac3-polyclonal-antibody-tds',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-10-24 15:37:26',
'created' => '2018-10-24 15:37:26',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1783',
'name' => 'product/antibodies/chipseq-grade-ab-icon.png',
'alt' => 'ChIP-seq Grade',
'modified' => '2020-11-27 07:04:40',
'created' => '2018-03-15 15:54:09',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '3812',
'name' => 'SDS C15410364 HDAC3 Antibody US en',
'language' => 'en',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-US-en-GHS_2_0.pdf',
'countries' => 'US',
'modified' => '2024-01-17 19:46:35',
'created' => '2024-01-17 19:46:35',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '3814',
'name' => 'SDS C15410364 HDAC3 Antibody GB en',
'language' => 'en',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2024-01-17 19:47:10',
'created' => '2024-01-17 19:47:10',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '3813',
'name' => 'SDS C15410364 HDAC3 Antibody JP ja',
'language' => 'ja',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2024-01-17 19:46:53',
'created' => '2024-01-17 19:46:53',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3816',
'name' => 'SDS C15410364 HDAC3 Antibody ES es',
'language' => 'es',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2024-01-17 19:47:42',
'created' => '2024-01-17 19:47:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3815',
'name' => 'SDS C15410364 HDAC3 Antibody FR fr',
'language' => 'fr',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2024-01-17 19:47:26',
'created' => '2024-01-17 19:47:26',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '3811',
'name' => 'SDS C15410364 HDAC3 Antibody BE fr',
'language' => 'fr',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2024-01-17 19:46:13',
'created' => '2024-01-17 19:46:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '3810',
'name' => 'SDS C15410364 HDAC3 Antibody BE nl',
'language' => 'nl',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-BE-nl-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2024-01-17 19:45:58',
'created' => '2024-01-17 19:45:58',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '3817',
'name' => 'SDS C15410364 HDAC3 Antibody DE de',
'language' => 'de',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2024-01-17 19:47:58',
'created' => '2024-01-17 19:47:58',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/hdac3-polyclonal-antibody-50-ug'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '3000',
'antibody_id' => '548',
'name' => 'HDAC3 Antibody',
'description' => '<p><strong>Other names:</strong> HD3, RPD3, RPD3-2, SMAP45</p>
<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.</p>',
'label3' => '',
'info3' => '',
'format' => '50 µg',
'catalog_number' => 'C15410364',
'old_catalog_number' => 'C15410361',
'sf_code' => 'C15410364-D001-000581',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '0',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'December 13, 2019',
'slug' => 'hdac3-polyclonal-antibody-50-ug',
'meta_title' => 'HDAC3 Antibody - ChIP-seq Grade (C15410364) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'HDAC3 (Histone deacetylase 3) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: HD3, RPD3, RPD3-2, SMAP45.',
'modified' => '2024-01-17 19:48:28',
'created' => '2018-10-24 11:56:07'
)
)
$pro = array(
'id' => '3182',
'antibody_id' => '643',
'name' => 'HDAC3 Antibody (sample size)',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15410364-10',
'old_catalog_number' => '',
'sf_code' => 'C15410364-D001-000582',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_title' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2024-01-17 19:48:49',
'created' => '2020-12-30 14:47:56',
'ProductsGroup' => array(
'id' => '388',
'product_id' => '3182',
'group_id' => '345'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ' <span style="color:#CCC">(C15410361)</span>'
$country_code = 'US'
$other_format = array(
'id' => '3000',
'antibody_id' => '548',
'name' => 'HDAC3 Antibody',
'description' => '<p><strong>Other names:</strong> HD3, RPD3, RPD3-2, SMAP45</p>
<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.</p>',
'label3' => '',
'info3' => '',
'format' => '50 µg',
'catalog_number' => 'C15410364',
'old_catalog_number' => 'C15410361',
'sf_code' => 'C15410364-D001-000581',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '0',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'December 13, 2019',
'slug' => 'hdac3-polyclonal-antibody-50-ug',
'meta_title' => 'HDAC3 Antibody - ChIP-seq Grade (C15410364) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'HDAC3 (Histone deacetylase 3) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: HD3, RPD3, RPD3-2, SMAP45.',
'modified' => '2024-01-17 19:48:28',
'created' => '2018-10-24 11:56:07'
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
'id' => '5433',
'product_id' => '3182',
'application_id' => '43'
)
)
$slugs = array(
(int) 0 => 'chip-qpcr-antibodies'
)
$applications = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'locale' => 'zho'
)
$description = ''
$name = 'ChIP-qPCR (ab)'
$document = array(
'id' => '1036',
'name' => 'HDAC3 polyclonal antibody',
'description' => '<p>Polyclonal antibody raised in rabbit against human HDAC3 (Histone deacetylase 3), using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/hdac3-polyclonal-antibody-50-ug.pdf',
'slug' => 'hdac3-polyclonal-antibody-tds',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-10-24 15:37:26',
'created' => '2018-10-24 15:37:26',
'ProductsDocument' => array(
'id' => '3092',
'product_id' => '3182',
'document_id' => '1036'
)
)
$sds = array(
'id' => '3817',
'name' => 'SDS C15410364 HDAC3 Antibody DE de',
'language' => 'de',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2024-01-17 19:47:58',
'created' => '2024-01-17 19:47:58',
'ProductsSafetySheet' => array(
'id' => '6261',
'product_id' => '3182',
'safety_sheet_id' => '3817'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'cn',
'meta_keywords' => '',
'meta_description' => '',
'meta_title' => '',
'product' => array(
'Product' => array(
'id' => '3182',
'antibody_id' => '643',
'name' => 'HDAC3 Antibody - ChIP-seq Grade (sample size)',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15410364-10',
'old_catalog_number' => '',
'sf_code' => 'C15410364-D001-000582',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_title' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2024-01-17 19:48:49',
'created' => '2020-12-30 14:47:56',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '643',
'name' => 'HDAC3 Antibody - ChIP-seq Grade (sample size)',
'description' => 'HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.',
'clonality' => '',
'isotype' => '',
'lot' => 'A2633-0040',
'concentration' => '1.7 µg/µl',
'reactivity' => 'Human: positive. Other species: not tested.',
'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>',
'purity' => 'Affinity purified polyclonal antibody.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 µg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:10,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>Not recommended</td>
<td></td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => 'PBS containing 0.05% azide.',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-12-30 14:43:44',
'created' => '2020-12-30 14:43:44',
'select_label' => '643 - HDAC3 Antibody - ChIP-seq Grade (sample size) (A2633-0040 - 1.7 µg/µl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/hdac3-polyclonal-antibody-50-ug'
)
$language = 'cn'
$meta_keywords = ''
$meta_description = ''
$meta_title = ''
$product = array(
'Product' => array(
'id' => '3182',
'antibody_id' => '643',
'name' => 'HDAC3 Antibody - ChIP-seq Grade (sample size)',
'description' => '<p><strong>Other names:</strong> HD3, RPD3, RPD3-2, SMAP45</p>
<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.</p>',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15410364-10',
'old_catalog_number' => '',
'sf_code' => 'C15410364-D001-000582',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_title' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2024-01-17 19:48:49',
'created' => '2020-12-30 14:47:56',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '643',
'name' => 'HDAC3 Antibody - ChIP-seq Grade (sample size)',
'description' => 'HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.',
'clonality' => '',
'isotype' => '',
'lot' => 'A2633-0040',
'concentration' => '1.7 µg/µl',
'reactivity' => 'Human: positive. Other species: not tested.',
'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>',
'purity' => 'Affinity purified polyclonal antibody.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 µg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:10,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>Not recommended</td>
<td></td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => 'PBS containing 0.05% azide.',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-12-30 14:43:44',
'created' => '2020-12-30 14:43:44',
'select_label' => '643 - HDAC3 Antibody - ChIP-seq Grade (sample size) (A2633-0040 - 1.7 µg/µl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '345',
'name' => 'C15410364',
'product_id' => '3000',
'modified' => '2020-12-30 14:48:18',
'created' => '2020-12-30 14:48:18'
),
'Master' => array(
'id' => '3000',
'antibody_id' => '548',
'name' => 'HDAC3 Antibody',
'description' => '<p><strong>Other names:</strong> HD3, RPD3, RPD3-2, SMAP45</p>
<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.</p>',
'label3' => '',
'info3' => '',
'format' => '50 µg',
'catalog_number' => 'C15410364',
'old_catalog_number' => 'C15410361',
'sf_code' => 'C15410364-D001-000581',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '0',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'December 13, 2019',
'slug' => 'hdac3-polyclonal-antibody-50-ug',
'meta_title' => 'HDAC3 Antibody - ChIP-seq Grade (C15410364) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'HDAC3 (Histone deacetylase 3) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: HD3, RPD3, RPD3-2, SMAP45.',
'modified' => '2024-01-17 19:48:28',
'created' => '2018-10-24 11:56:07'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '20',
'position' => '10',
'parent_id' => '40',
'name' => 'ELISA',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
</div>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'elisa-antibodies',
'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody',
'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications',
'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:21:41',
'created' => '2014-07-08 08:13:28',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '42',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-seq (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-seq-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications',
'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode',
'modified' => '2016-01-20 11:06:19',
'created' => '2015-10-20 11:44:45',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '17',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-seq grade antibodies',
'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode’s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-seq-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)',
'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:22',
'created' => '2015-02-16 02:24:01',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '127',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-grade antibodies',
'description' => '<div class="row">
<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode',
'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
'modified' => '2024-11-19 17:27:07',
'created' => '2017-02-14 11:16:04',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 2 => array(
'id' => '21',
'position' => '20',
'parent_id' => '4',
'name' => 'Histone modifying enzymes',
'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chromatin-modifying-proteins-histone-transferase',
'cookies_tag_id' => null,
'meta_keywords' => 'monoclonal antibodies,policlonal antibodies,Histone modifying enzymes',
'meta_description' => 'Diagenode Offers Polyclonal and Monoclonal Antibodies Against Histone Modifying Enzymes like: Histone Deacetylases, Histone Demethylases, Histone Transferases.',
'meta_title' => 'Histone modifying enzymes - Antibodies | Diagenode',
'modified' => '2019-07-04 16:19:15',
'created' => '2014-06-26 13:18:41',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 3 => array(
'id' => '102',
'position' => '1',
'parent_id' => '4',
'name' => 'Sample size antibodies',
'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => true,
'is_antibody' => true,
'slug' => 'sample-size-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
'meta_title' => 'Sample-size Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:05',
'created' => '2015-10-27 12:13:34',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 4 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1036',
'name' => 'HDAC3 polyclonal antibody',
'description' => '<p>Polyclonal antibody raised in rabbit against human HDAC3 (Histone deacetylase 3), using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/hdac3-polyclonal-antibody-50-ug.pdf',
'slug' => 'hdac3-polyclonal-antibody-tds',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-10-24 15:37:26',
'created' => '2018-10-24 15:37:26',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1783',
'name' => 'product/antibodies/chipseq-grade-ab-icon.png',
'alt' => 'ChIP-seq Grade',
'modified' => '2020-11-27 07:04:40',
'created' => '2018-03-15 15:54:09',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '3812',
'name' => 'SDS C15410364 HDAC3 Antibody US en',
'language' => 'en',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-US-en-GHS_2_0.pdf',
'countries' => 'US',
'modified' => '2024-01-17 19:46:35',
'created' => '2024-01-17 19:46:35',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '3814',
'name' => 'SDS C15410364 HDAC3 Antibody GB en',
'language' => 'en',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2024-01-17 19:47:10',
'created' => '2024-01-17 19:47:10',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '3813',
'name' => 'SDS C15410364 HDAC3 Antibody JP ja',
'language' => 'ja',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2024-01-17 19:46:53',
'created' => '2024-01-17 19:46:53',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3816',
'name' => 'SDS C15410364 HDAC3 Antibody ES es',
'language' => 'es',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2024-01-17 19:47:42',
'created' => '2024-01-17 19:47:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3815',
'name' => 'SDS C15410364 HDAC3 Antibody FR fr',
'language' => 'fr',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2024-01-17 19:47:26',
'created' => '2024-01-17 19:47:26',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '3811',
'name' => 'SDS C15410364 HDAC3 Antibody BE fr',
'language' => 'fr',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2024-01-17 19:46:13',
'created' => '2024-01-17 19:46:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '3810',
'name' => 'SDS C15410364 HDAC3 Antibody BE nl',
'language' => 'nl',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-BE-nl-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2024-01-17 19:45:58',
'created' => '2024-01-17 19:45:58',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '3817',
'name' => 'SDS C15410364 HDAC3 Antibody DE de',
'language' => 'de',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2024-01-17 19:47:58',
'created' => '2024-01-17 19:47:58',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/hdac3-polyclonal-antibody-50-ug'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '3000',
'antibody_id' => '548',
'name' => 'HDAC3 Antibody',
'description' => '<p><strong>Other names:</strong> HD3, RPD3, RPD3-2, SMAP45</p>
<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.</p>',
'label3' => '',
'info3' => '',
'format' => '50 µg',
'catalog_number' => 'C15410364',
'old_catalog_number' => 'C15410361',
'sf_code' => 'C15410364-D001-000581',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '0',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'December 13, 2019',
'slug' => 'hdac3-polyclonal-antibody-50-ug',
'meta_title' => 'HDAC3 Antibody - ChIP-seq Grade (C15410364) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'HDAC3 (Histone deacetylase 3) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: HD3, RPD3, RPD3-2, SMAP45.',
'modified' => '2024-01-17 19:48:28',
'created' => '2018-10-24 11:56:07'
)
)
$pro = array(
'id' => '3182',
'antibody_id' => '643',
'name' => 'HDAC3 Antibody (sample size)',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15410364-10',
'old_catalog_number' => '',
'sf_code' => 'C15410364-D001-000582',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_title' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2024-01-17 19:48:49',
'created' => '2020-12-30 14:47:56',
'ProductsGroup' => array(
'id' => '388',
'product_id' => '3182',
'group_id' => '345'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ' <span style="color:#CCC">(C15410361)</span>'
$country_code = 'US'
$other_format = array(
'id' => '3000',
'antibody_id' => '548',
'name' => 'HDAC3 Antibody',
'description' => '<p><strong>Other names:</strong> HD3, RPD3, RPD3-2, SMAP45</p>
<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.</p>',
'label3' => '',
'info3' => '',
'format' => '50 µg',
'catalog_number' => 'C15410364',
'old_catalog_number' => 'C15410361',
'sf_code' => 'C15410364-D001-000581',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '0',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'December 13, 2019',
'slug' => 'hdac3-polyclonal-antibody-50-ug',
'meta_title' => 'HDAC3 Antibody - ChIP-seq Grade (C15410364) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'HDAC3 (Histone deacetylase 3) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: HD3, RPD3, RPD3-2, SMAP45.',
'modified' => '2024-01-17 19:48:28',
'created' => '2018-10-24 11:56:07'
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
'id' => '5433',
'product_id' => '3182',
'application_id' => '43'
)
)
$slugs = array(
(int) 0 => 'chip-qpcr-antibodies'
)
$applications = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'locale' => 'zho'
)
$description = ''
$name = 'ChIP-qPCR (ab)'
$document = array(
'id' => '1036',
'name' => 'HDAC3 polyclonal antibody',
'description' => '<p>Polyclonal antibody raised in rabbit against human HDAC3 (Histone deacetylase 3), using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/hdac3-polyclonal-antibody-50-ug.pdf',
'slug' => 'hdac3-polyclonal-antibody-tds',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-10-24 15:37:26',
'created' => '2018-10-24 15:37:26',
'ProductsDocument' => array(
'id' => '3092',
'product_id' => '3182',
'document_id' => '1036'
)
)
$sds = array(
'id' => '3817',
'name' => 'SDS C15410364 HDAC3 Antibody DE de',
'language' => 'de',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2024-01-17 19:47:58',
'created' => '2024-01-17 19:47:58',
'ProductsSafetySheet' => array(
'id' => '6261',
'product_id' => '3182',
'safety_sheet_id' => '3817'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'cn',
'meta_keywords' => '',
'meta_description' => '',
'meta_title' => '',
'product' => array(
'Product' => array(
'id' => '3182',
'antibody_id' => '643',
'name' => 'HDAC3 Antibody - ChIP-seq Grade (sample size)',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15410364-10',
'old_catalog_number' => '',
'sf_code' => 'C15410364-D001-000582',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_title' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2024-01-17 19:48:49',
'created' => '2020-12-30 14:47:56',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '643',
'name' => 'HDAC3 Antibody - ChIP-seq Grade (sample size)',
'description' => 'HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.',
'clonality' => '',
'isotype' => '',
'lot' => 'A2633-0040',
'concentration' => '1.7 µg/µl',
'reactivity' => 'Human: positive. Other species: not tested.',
'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>',
'purity' => 'Affinity purified polyclonal antibody.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 µg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:10,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>Not recommended</td>
<td></td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => 'PBS containing 0.05% azide.',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-12-30 14:43:44',
'created' => '2020-12-30 14:43:44',
'select_label' => '643 - HDAC3 Antibody - ChIP-seq Grade (sample size) (A2633-0040 - 1.7 µg/µl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/hdac3-polyclonal-antibody-50-ug'
)
$language = 'cn'
$meta_keywords = ''
$meta_description = ''
$meta_title = ''
$product = array(
'Product' => array(
'id' => '3182',
'antibody_id' => '643',
'name' => 'HDAC3 Antibody - ChIP-seq Grade (sample size)',
'description' => '<p><strong>Other names:</strong> HD3, RPD3, RPD3-2, SMAP45</p>
<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.</p>',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15410364-10',
'old_catalog_number' => '',
'sf_code' => 'C15410364-D001-000582',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_title' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2024-01-17 19:48:49',
'created' => '2020-12-30 14:47:56',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '643',
'name' => 'HDAC3 Antibody - ChIP-seq Grade (sample size)',
'description' => 'HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.',
'clonality' => '',
'isotype' => '',
'lot' => 'A2633-0040',
'concentration' => '1.7 µg/µl',
'reactivity' => 'Human: positive. Other species: not tested.',
'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>',
'purity' => 'Affinity purified polyclonal antibody.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 µg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:10,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>Not recommended</td>
<td></td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => 'PBS containing 0.05% azide.',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-12-30 14:43:44',
'created' => '2020-12-30 14:43:44',
'select_label' => '643 - HDAC3 Antibody - ChIP-seq Grade (sample size) (A2633-0040 - 1.7 µg/µl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '345',
'name' => 'C15410364',
'product_id' => '3000',
'modified' => '2020-12-30 14:48:18',
'created' => '2020-12-30 14:48:18'
),
'Master' => array(
'id' => '3000',
'antibody_id' => '548',
'name' => 'HDAC3 Antibody',
'description' => '<p><strong>Other names:</strong> HD3, RPD3, RPD3-2, SMAP45</p>
<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.</p>',
'label3' => '',
'info3' => '',
'format' => '50 µg',
'catalog_number' => 'C15410364',
'old_catalog_number' => 'C15410361',
'sf_code' => 'C15410364-D001-000581',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '0',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'December 13, 2019',
'slug' => 'hdac3-polyclonal-antibody-50-ug',
'meta_title' => 'HDAC3 Antibody - ChIP-seq Grade (C15410364) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'HDAC3 (Histone deacetylase 3) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: HD3, RPD3, RPD3-2, SMAP45.',
'modified' => '2024-01-17 19:48:28',
'created' => '2018-10-24 11:56:07'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '20',
'position' => '10',
'parent_id' => '40',
'name' => 'ELISA',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
</div>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'elisa-antibodies',
'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody',
'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications',
'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:21:41',
'created' => '2014-07-08 08:13:28',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '42',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-seq (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-seq-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications',
'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode',
'modified' => '2016-01-20 11:06:19',
'created' => '2015-10-20 11:44:45',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '17',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-seq grade antibodies',
'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode’s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-seq-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)',
'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:22',
'created' => '2015-02-16 02:24:01',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '127',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-grade antibodies',
'description' => '<div class="row">
<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode',
'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
'modified' => '2024-11-19 17:27:07',
'created' => '2017-02-14 11:16:04',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 2 => array(
'id' => '21',
'position' => '20',
'parent_id' => '4',
'name' => 'Histone modifying enzymes',
'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chromatin-modifying-proteins-histone-transferase',
'cookies_tag_id' => null,
'meta_keywords' => 'monoclonal antibodies,policlonal antibodies,Histone modifying enzymes',
'meta_description' => 'Diagenode Offers Polyclonal and Monoclonal Antibodies Against Histone Modifying Enzymes like: Histone Deacetylases, Histone Demethylases, Histone Transferases.',
'meta_title' => 'Histone modifying enzymes - Antibodies | Diagenode',
'modified' => '2019-07-04 16:19:15',
'created' => '2014-06-26 13:18:41',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 3 => array(
'id' => '102',
'position' => '1',
'parent_id' => '4',
'name' => 'Sample size antibodies',
'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => true,
'is_antibody' => true,
'slug' => 'sample-size-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
'meta_title' => 'Sample-size Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:05',
'created' => '2015-10-27 12:13:34',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 4 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1036',
'name' => 'HDAC3 polyclonal antibody',
'description' => '<p>Polyclonal antibody raised in rabbit against human HDAC3 (Histone deacetylase 3), using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/hdac3-polyclonal-antibody-50-ug.pdf',
'slug' => 'hdac3-polyclonal-antibody-tds',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-10-24 15:37:26',
'created' => '2018-10-24 15:37:26',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1783',
'name' => 'product/antibodies/chipseq-grade-ab-icon.png',
'alt' => 'ChIP-seq Grade',
'modified' => '2020-11-27 07:04:40',
'created' => '2018-03-15 15:54:09',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '3812',
'name' => 'SDS C15410364 HDAC3 Antibody US en',
'language' => 'en',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-US-en-GHS_2_0.pdf',
'countries' => 'US',
'modified' => '2024-01-17 19:46:35',
'created' => '2024-01-17 19:46:35',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '3814',
'name' => 'SDS C15410364 HDAC3 Antibody GB en',
'language' => 'en',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2024-01-17 19:47:10',
'created' => '2024-01-17 19:47:10',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '3813',
'name' => 'SDS C15410364 HDAC3 Antibody JP ja',
'language' => 'ja',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2024-01-17 19:46:53',
'created' => '2024-01-17 19:46:53',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3816',
'name' => 'SDS C15410364 HDAC3 Antibody ES es',
'language' => 'es',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2024-01-17 19:47:42',
'created' => '2024-01-17 19:47:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3815',
'name' => 'SDS C15410364 HDAC3 Antibody FR fr',
'language' => 'fr',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2024-01-17 19:47:26',
'created' => '2024-01-17 19:47:26',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '3811',
'name' => 'SDS C15410364 HDAC3 Antibody BE fr',
'language' => 'fr',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2024-01-17 19:46:13',
'created' => '2024-01-17 19:46:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '3810',
'name' => 'SDS C15410364 HDAC3 Antibody BE nl',
'language' => 'nl',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-BE-nl-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2024-01-17 19:45:58',
'created' => '2024-01-17 19:45:58',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '3817',
'name' => 'SDS C15410364 HDAC3 Antibody DE de',
'language' => 'de',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2024-01-17 19:47:58',
'created' => '2024-01-17 19:47:58',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/hdac3-polyclonal-antibody-50-ug'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '3000',
'antibody_id' => '548',
'name' => 'HDAC3 Antibody',
'description' => '<p><strong>Other names:</strong> HD3, RPD3, RPD3-2, SMAP45</p>
<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.</p>',
'label3' => '',
'info3' => '',
'format' => '50 µg',
'catalog_number' => 'C15410364',
'old_catalog_number' => 'C15410361',
'sf_code' => 'C15410364-D001-000581',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '0',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'December 13, 2019',
'slug' => 'hdac3-polyclonal-antibody-50-ug',
'meta_title' => 'HDAC3 Antibody - ChIP-seq Grade (C15410364) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'HDAC3 (Histone deacetylase 3) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: HD3, RPD3, RPD3-2, SMAP45.',
'modified' => '2024-01-17 19:48:28',
'created' => '2018-10-24 11:56:07'
)
)
$pro = array(
'id' => '3182',
'antibody_id' => '643',
'name' => 'HDAC3 Antibody (sample size)',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15410364-10',
'old_catalog_number' => '',
'sf_code' => 'C15410364-D001-000582',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_title' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2024-01-17 19:48:49',
'created' => '2020-12-30 14:47:56',
'ProductsGroup' => array(
'id' => '388',
'product_id' => '3182',
'group_id' => '345'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ' <span style="color:#CCC">(C15410361)</span>'
$country_code = 'US'
$other_format = array(
'id' => '3000',
'antibody_id' => '548',
'name' => 'HDAC3 Antibody',
'description' => '<p><strong>Other names:</strong> HD3, RPD3, RPD3-2, SMAP45</p>
<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.</p>',
'label3' => '',
'info3' => '',
'format' => '50 µg',
'catalog_number' => 'C15410364',
'old_catalog_number' => 'C15410361',
'sf_code' => 'C15410364-D001-000581',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '0',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'December 13, 2019',
'slug' => 'hdac3-polyclonal-antibody-50-ug',
'meta_title' => 'HDAC3 Antibody - ChIP-seq Grade (C15410364) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'HDAC3 (Histone deacetylase 3) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: HD3, RPD3, RPD3-2, SMAP45.',
'modified' => '2024-01-17 19:48:28',
'created' => '2018-10-24 11:56:07'
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
'id' => '5433',
'product_id' => '3182',
'application_id' => '43'
)
)
$slugs = array(
(int) 0 => 'chip-qpcr-antibodies'
)
$applications = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'locale' => 'zho'
)
$description = ''
$name = 'ChIP-qPCR (ab)'
$document = array(
'id' => '1036',
'name' => 'HDAC3 polyclonal antibody',
'description' => '<p>Polyclonal antibody raised in rabbit against human HDAC3 (Histone deacetylase 3), using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/hdac3-polyclonal-antibody-50-ug.pdf',
'slug' => 'hdac3-polyclonal-antibody-tds',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-10-24 15:37:26',
'created' => '2018-10-24 15:37:26',
'ProductsDocument' => array(
'id' => '3092',
'product_id' => '3182',
'document_id' => '1036'
)
)
$sds = array(
'id' => '3817',
'name' => 'SDS C15410364 HDAC3 Antibody DE de',
'language' => 'de',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2024-01-17 19:47:58',
'created' => '2024-01-17 19:47:58',
'ProductsSafetySheet' => array(
'id' => '6261',
'product_id' => '3182',
'safety_sheet_id' => '3817'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'cn',
'meta_keywords' => '',
'meta_description' => '',
'meta_title' => '',
'product' => array(
'Product' => array(
'id' => '3182',
'antibody_id' => '643',
'name' => 'HDAC3 Antibody - ChIP-seq Grade (sample size)',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15410364-10',
'old_catalog_number' => '',
'sf_code' => 'C15410364-D001-000582',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_title' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2024-01-17 19:48:49',
'created' => '2020-12-30 14:47:56',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '643',
'name' => 'HDAC3 Antibody - ChIP-seq Grade (sample size)',
'description' => 'HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.',
'clonality' => '',
'isotype' => '',
'lot' => 'A2633-0040',
'concentration' => '1.7 µg/µl',
'reactivity' => 'Human: positive. Other species: not tested.',
'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>',
'purity' => 'Affinity purified polyclonal antibody.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 µg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:10,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>Not recommended</td>
<td></td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => 'PBS containing 0.05% azide.',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-12-30 14:43:44',
'created' => '2020-12-30 14:43:44',
'select_label' => '643 - HDAC3 Antibody - ChIP-seq Grade (sample size) (A2633-0040 - 1.7 µg/µl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/hdac3-polyclonal-antibody-50-ug'
)
$language = 'cn'
$meta_keywords = ''
$meta_description = ''
$meta_title = ''
$product = array(
'Product' => array(
'id' => '3182',
'antibody_id' => '643',
'name' => 'HDAC3 Antibody - ChIP-seq Grade (sample size)',
'description' => '<p><strong>Other names:</strong> HD3, RPD3, RPD3-2, SMAP45</p>
<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.</p>',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15410364-10',
'old_catalog_number' => '',
'sf_code' => 'C15410364-D001-000582',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_title' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2024-01-17 19:48:49',
'created' => '2020-12-30 14:47:56',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '643',
'name' => 'HDAC3 Antibody - ChIP-seq Grade (sample size)',
'description' => 'HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.',
'clonality' => '',
'isotype' => '',
'lot' => 'A2633-0040',
'concentration' => '1.7 µg/µl',
'reactivity' => 'Human: positive. Other species: not tested.',
'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>',
'purity' => 'Affinity purified polyclonal antibody.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 µg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:10,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>Not recommended</td>
<td></td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => 'PBS containing 0.05% azide.',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-12-30 14:43:44',
'created' => '2020-12-30 14:43:44',
'select_label' => '643 - HDAC3 Antibody - ChIP-seq Grade (sample size) (A2633-0040 - 1.7 µg/µl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '345',
'name' => 'C15410364',
'product_id' => '3000',
'modified' => '2020-12-30 14:48:18',
'created' => '2020-12-30 14:48:18'
),
'Master' => array(
'id' => '3000',
'antibody_id' => '548',
'name' => 'HDAC3 Antibody',
'description' => '<p><strong>Other names:</strong> HD3, RPD3, RPD3-2, SMAP45</p>
<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.</p>',
'label3' => '',
'info3' => '',
'format' => '50 µg',
'catalog_number' => 'C15410364',
'old_catalog_number' => 'C15410361',
'sf_code' => 'C15410364-D001-000581',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '0',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'December 13, 2019',
'slug' => 'hdac3-polyclonal-antibody-50-ug',
'meta_title' => 'HDAC3 Antibody - ChIP-seq Grade (C15410364) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'HDAC3 (Histone deacetylase 3) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: HD3, RPD3, RPD3-2, SMAP45.',
'modified' => '2024-01-17 19:48:28',
'created' => '2018-10-24 11:56:07'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '20',
'position' => '10',
'parent_id' => '40',
'name' => 'ELISA',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
</div>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'elisa-antibodies',
'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody',
'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications',
'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:21:41',
'created' => '2014-07-08 08:13:28',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '42',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-seq (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-seq-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications',
'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode',
'modified' => '2016-01-20 11:06:19',
'created' => '2015-10-20 11:44:45',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '17',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-seq grade antibodies',
'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode’s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-seq-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)',
'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:22',
'created' => '2015-02-16 02:24:01',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '127',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-grade antibodies',
'description' => '<div class="row">
<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode',
'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
'modified' => '2024-11-19 17:27:07',
'created' => '2017-02-14 11:16:04',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 2 => array(
'id' => '21',
'position' => '20',
'parent_id' => '4',
'name' => 'Histone modifying enzymes',
'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chromatin-modifying-proteins-histone-transferase',
'cookies_tag_id' => null,
'meta_keywords' => 'monoclonal antibodies,policlonal antibodies,Histone modifying enzymes',
'meta_description' => 'Diagenode Offers Polyclonal and Monoclonal Antibodies Against Histone Modifying Enzymes like: Histone Deacetylases, Histone Demethylases, Histone Transferases.',
'meta_title' => 'Histone modifying enzymes - Antibodies | Diagenode',
'modified' => '2019-07-04 16:19:15',
'created' => '2014-06-26 13:18:41',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 3 => array(
'id' => '102',
'position' => '1',
'parent_id' => '4',
'name' => 'Sample size antibodies',
'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => true,
'is_antibody' => true,
'slug' => 'sample-size-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
'meta_title' => 'Sample-size Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:05',
'created' => '2015-10-27 12:13:34',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 4 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1036',
'name' => 'HDAC3 polyclonal antibody',
'description' => '<p>Polyclonal antibody raised in rabbit against human HDAC3 (Histone deacetylase 3), using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/hdac3-polyclonal-antibody-50-ug.pdf',
'slug' => 'hdac3-polyclonal-antibody-tds',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-10-24 15:37:26',
'created' => '2018-10-24 15:37:26',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1783',
'name' => 'product/antibodies/chipseq-grade-ab-icon.png',
'alt' => 'ChIP-seq Grade',
'modified' => '2020-11-27 07:04:40',
'created' => '2018-03-15 15:54:09',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '3812',
'name' => 'SDS C15410364 HDAC3 Antibody US en',
'language' => 'en',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-US-en-GHS_2_0.pdf',
'countries' => 'US',
'modified' => '2024-01-17 19:46:35',
'created' => '2024-01-17 19:46:35',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '3814',
'name' => 'SDS C15410364 HDAC3 Antibody GB en',
'language' => 'en',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2024-01-17 19:47:10',
'created' => '2024-01-17 19:47:10',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '3813',
'name' => 'SDS C15410364 HDAC3 Antibody JP ja',
'language' => 'ja',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2024-01-17 19:46:53',
'created' => '2024-01-17 19:46:53',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3816',
'name' => 'SDS C15410364 HDAC3 Antibody ES es',
'language' => 'es',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2024-01-17 19:47:42',
'created' => '2024-01-17 19:47:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3815',
'name' => 'SDS C15410364 HDAC3 Antibody FR fr',
'language' => 'fr',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2024-01-17 19:47:26',
'created' => '2024-01-17 19:47:26',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '3811',
'name' => 'SDS C15410364 HDAC3 Antibody BE fr',
'language' => 'fr',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2024-01-17 19:46:13',
'created' => '2024-01-17 19:46:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '3810',
'name' => 'SDS C15410364 HDAC3 Antibody BE nl',
'language' => 'nl',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-BE-nl-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2024-01-17 19:45:58',
'created' => '2024-01-17 19:45:58',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '3817',
'name' => 'SDS C15410364 HDAC3 Antibody DE de',
'language' => 'de',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2024-01-17 19:47:58',
'created' => '2024-01-17 19:47:58',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/hdac3-polyclonal-antibody-50-ug'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '3000',
'antibody_id' => '548',
'name' => 'HDAC3 Antibody',
'description' => '<p><strong>Other names:</strong> HD3, RPD3, RPD3-2, SMAP45</p>
<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.</p>',
'label3' => '',
'info3' => '',
'format' => '50 µg',
'catalog_number' => 'C15410364',
'old_catalog_number' => 'C15410361',
'sf_code' => 'C15410364-D001-000581',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '0',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'December 13, 2019',
'slug' => 'hdac3-polyclonal-antibody-50-ug',
'meta_title' => 'HDAC3 Antibody - ChIP-seq Grade (C15410364) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'HDAC3 (Histone deacetylase 3) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: HD3, RPD3, RPD3-2, SMAP45.',
'modified' => '2024-01-17 19:48:28',
'created' => '2018-10-24 11:56:07'
)
)
$pro = array(
'id' => '3182',
'antibody_id' => '643',
'name' => 'HDAC3 Antibody (sample size)',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15410364-10',
'old_catalog_number' => '',
'sf_code' => 'C15410364-D001-000582',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'hdac3-polyclonal-antibody-10',
'meta_title' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2024-01-17 19:48:49',
'created' => '2020-12-30 14:47:56',
'ProductsGroup' => array(
'id' => '388',
'product_id' => '3182',
'group_id' => '345'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ' <span style="color:#CCC">(C15410361)</span>'
$country_code = 'US'
$other_format = array(
'id' => '3000',
'antibody_id' => '548',
'name' => 'HDAC3 Antibody',
'description' => '<p><strong>Other names:</strong> HD3, RPD3, RPD3-2, SMAP45</p>
<p>Polyclonal antibody raised in rabbit against human <strong>HDAC3 (Histone deacetylase 3)</strong>, using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-chip.jpg" alt="HDAC3 Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-a.jpg" alt="HDAC3 Antibody ChIP-seq Grade" caption="false" width="700" height="84" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-b.jpg" alt="HDAC3 Antibody for ChIP-seq" caption="false" width="700" height="182" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-c.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="164" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-d.jpg" alt="HDAC3 Antibody for ChIP-seq assay" caption="false" width="700" height="142" /></p>
<p>E.<img src="https://www.diagenode.com/img/product/antibodies/C15410361-chipseq-e.jpg" alt="HDAC3 Antibody validated in ChIP-seq" caption="false" width="700" height="154" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3</strong><br />ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410361-elisa.jpg" alt="HDAC3 Antibody ELISA validation" caption="false" width="447" height="339" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.</p>',
'label3' => '',
'info3' => '',
'format' => '50 µg',
'catalog_number' => 'C15410364',
'old_catalog_number' => 'C15410361',
'sf_code' => 'C15410364-D001-000581',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '0',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'December 13, 2019',
'slug' => 'hdac3-polyclonal-antibody-50-ug',
'meta_title' => 'HDAC3 Antibody - ChIP-seq Grade (C15410364) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'HDAC3 (Histone deacetylase 3) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: HD3, RPD3, RPD3-2, SMAP45.',
'modified' => '2024-01-17 19:48:28',
'created' => '2018-10-24 11:56:07'
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
'id' => '5433',
'product_id' => '3182',
'application_id' => '43'
)
)
$slugs = array(
(int) 0 => 'chip-qpcr-antibodies'
)
$applications = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'locale' => 'zho'
)
$description = ''
$name = 'ChIP-qPCR (ab)'
$document = array(
'id' => '1036',
'name' => 'HDAC3 polyclonal antibody',
'description' => '<p>Polyclonal antibody raised in rabbit against human HDAC3 (Histone deacetylase 3), using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/hdac3-polyclonal-antibody-50-ug.pdf',
'slug' => 'hdac3-polyclonal-antibody-tds',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-10-24 15:37:26',
'created' => '2018-10-24 15:37:26',
'ProductsDocument' => array(
'id' => '3092',
'product_id' => '3182',
'document_id' => '1036'
)
)
$sds = array(
'id' => '3817',
'name' => 'SDS C15410364 HDAC3 Antibody DE de',
'language' => 'de',
'url' => 'files/SDS/HDAC3/SDS-C15410364-HDAC3_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2024-01-17 19:47:58',
'created' => '2024-01-17 19:47:58',
'ProductsSafetySheet' => array(
'id' => '6261',
'product_id' => '3182',
'safety_sheet_id' => '3817'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
×
