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Figure 1. Western blot analysis using the Diagenode antibody directed against LSD1 Figure 1A: Western blot was performed with the Diagenode antibody against LSD1 (cat# CS-028-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk on purified recombinant LSD1 protein (50 and 25 ng, lane 1 and 2) and on nuclear extracts (50 μg) from HeLa and HEK293 cells (lane 3 and 4, respectively). Figure 1B: Western blot was performed as described above using nuclear extracts from HeLa cells (HeLa NE, 20 μg). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
Figure 2. Western blot analysis using the Diagenode antibody directed against LSD1 Whole cell extracts (40 μg) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (cat. No. C15310028) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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siRNA Knockdown
Epigenetic antibodies you can trust!
Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level o... Read more