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<p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse MBD2 (Cat. No. CS-098-100). By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:185,000.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD2 (1)</strong><br /> Western blot was performed on nuclear extracts from mouse fibroblastst (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse MBD2 (Cat. No. CS-098-100), diluted 1:500 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD2 (1)</strong><br /> Western blot was performed on nuclear extracts from mouse fibroblastst (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse MBD2 (Cat. No. CS-098-100), diluted 1:500 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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