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<p>The Megaruptor® 3 was designed to provide the best experience with the fragmentation of DNA from <strong>5 kb - 100 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly system allows for <strong>8 samples to be processed simultaneously </strong>without additional user input. A <a href="https://www.diagenode.com/p/megaruptor-cassette-12">12 sample cassette</a> <span style="font-weight: 400;">is also available for purchase separately for increased throughput. </span>Just set the desired parameters and the automated system takes care of the rest. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>
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<div style="padding: 30px; background-color: #dad6e3;">
<p style="font-size: 1.2em; color: #2b2967;"><strong>Discover the NEW Megaruptor 3 HT</strong></p>
<p>The <strong>Megaruptor 3 HT</strong> offers an <strong>ultrafast protocol</strong> for increased sample processing speed to accommodate high-throughput sequencing projects. When using the ultrafast protocol, the <strong>Megaruptor 3 HT allows shearing of 96 samples in 2h into fragments of 7 to 40 kb</strong>.</p>
<p><strong>The NEW Megaruptor 3 HT will give you:</strong></p>
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<li><strong>High throughput:</strong> Processes 96 samples in 2 h.</li>
<li><strong>Flexibility:</strong> Allows the fragment size range (within 7 to 40 kb) to be chosen for hands-free operation.</li>
<li><strong>Unmatched quality:</strong> Provides the tight DNA fragment distribution necessary for high quality long-read sequencing.</li>
<li><strong>Reliable:</strong> enables processing of highly viscous samples.</li>
<li><strong>Ergonomic:</strong> Includes a user-friendly interface and has a small footprint.</li>
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<center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center><center></center><center><iframe width="700" height="394" src="https://www.youtube.com/embed/VaWEK1vjbOY?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center>
<p><a href="https://www.diagenode.com/en/documents/pacbio-megaruptor-application-note"><img src="https://www.diagenode.com/img/banners/pacbio-mega-banner.png" /></a></p>',
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<center><img src="https://www.diagenode.com/img/product/shearing_technologies/mega3-results.png" /></center>
<p><strong>A</strong>: Fragment Analyzer profiles of human genomic DNA samples (25 ng/μl; 200 μl/sample) sheared to 6, 10 and 30 kb. <strong>B</strong>: DNA samples sheared at different speed settings of the Megaruptor were analyzed by Pulsed Field Gel Electrophoresis (PFGE) in 1% agarose gel. (NS: Not Sheared)</p>',
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<th class="text-center" style="width: 296px; height: 55px;"></th>
<th class="text-center" style="width: 255px; height: 55px; background-color: #2b2967; color: white;">Megaruptor 3 regular</th>
<th class="text-center" style="width: 381px; height: 55px; background-color: #af3db2; color: white;">Megaruptor 3 HT with ultrafast protocol</th>
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<td style="width: 296px; height: 38px;">Run duration</td>
<td class="text-center" style="width: 255px; height: 38px;">20 min – few hours</td>
<td class="text-center" style="width: 381px; height: 38px;">1 – 5 min</td>
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<td class="text-center" style="width: 255px; height: 38px;">5 – 100 kb</td>
<td class="text-center" style="width: 381px; height: 38px;">7 – 40 kb</td>
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<td style="width: 296px; height: 38px;">Concentration</td>
<td class="text-center" style="width: 255px; height: 38px;">0 – 150 ng/μl</td>
<td class="text-center" style="width: 381px; height: 38px;">1 – 50 ng/μl</td>
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<td style="width: 296px; height: 38px;">Volume</td>
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<td class="text-center" style="width: 381px; height: 38px;">50 – 500 μl</td>
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<td class="text-center" style="width: 255px; height: 38px;">1 – 65</td>
<td class="text-center" style="width: 381px; height: 38px;">30 – 59</td>
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<td class="text-center" style="width: 255px; height: 56px;">96 samples in 1 day</td>
<td class="text-center" style="width: 381px; height: 56px;">96 samples in < 2h</td>
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<td style="width: 296px; height: 38px;">Shearing disposables</td>
<td class="text-center" style="width: 636px; height: 38px;" colspan="2">Multiple orifice hydrodynamic pore</td>
</tr>
<tr style="height: 32px;">
<td style="width: 296px; height: 32px;">Hardware</td>
<td class="text-center" style="width: 636px; height: 32px;" colspan="2">State-of-the-art electronics<br />Large memory for new features<br />Extensive fine-tuning options<br />Robust titanium pins</td>
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'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2>
<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
<p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p>',
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'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3>
<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<div class="small-12 columns" >
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<div class="small-12 columns" >
<h6 style="height:60px">Megaruptor 3 DNAFluid+ Kit</h6>
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<li>
<div class="row">
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<div style="padding: 30px; background-color: #dad6e3;">
<p style="font-size: 1.2em; color: #2b2967;"><strong>Discover the NEW Megaruptor 3 HT</strong></p>
<p>The <strong>Megaruptor 3 HT</strong> offers an <strong>ultrafast protocol</strong> for increased sample processing speed to accommodate high-throughput sequencing projects. When using the ultrafast protocol, the <strong>Megaruptor 3 HT allows shearing of 96 samples in 2h into fragments of 7 to 40 kb</strong>.</p>
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<li><strong>Unmatched quality:</strong> Provides the tight DNA fragment distribution necessary for high quality long-read sequencing.</li>
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<center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center><center></center><center><iframe width="700" height="394" src="https://www.youtube.com/embed/VaWEK1vjbOY?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center>
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<th class="text-center" style="width: 255px; height: 55px; background-color: #2b2967; color: white;">Megaruptor 3 regular</th>
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<td class="text-center" style="width: 636px; height: 32px;" colspan="2">State-of-the-art electronics<br />Large memory for new features<br />Extensive fine-tuning options<br />Robust titanium pins</td>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<div class="small-12 columns"><center><img src="../../img/partners/circulomics.png" /></center></div>
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'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2>
<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<div style="padding: 30px; background-color: #dad6e3;">
<p style="font-size: 1.2em; color: #2b2967;"><strong>Discover the NEW Megaruptor 3 HT</strong></p>
<p>The <strong>Megaruptor 3 HT</strong> offers an <strong>ultrafast protocol</strong> for increased sample processing speed to accommodate high-throughput sequencing projects. When using the ultrafast protocol, the <strong>Megaruptor 3 HT allows shearing of 96 samples in 2h into fragments of 7 to 40 kb</strong>.</p>
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<li><strong>Unmatched quality:</strong> Provides the tight DNA fragment distribution necessary for high quality long-read sequencing.</li>
<li><strong>Reliable:</strong> enables processing of highly viscous samples.</li>
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<center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center><center></center><center><iframe width="700" height="394" src="https://www.youtube.com/embed/VaWEK1vjbOY?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center>
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<th class="text-center" style="width: 296px; height: 55px;"></th>
<th class="text-center" style="width: 255px; height: 55px; background-color: #2b2967; color: white;">Megaruptor 3 regular</th>
<th class="text-center" style="width: 381px; height: 55px; background-color: #af3db2; color: white;">Megaruptor 3 HT with ultrafast protocol</th>
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<td class="text-center" style="width: 381px; height: 38px;">1 – 50 ng/μl</td>
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<td class="text-center" style="width: 255px; height: 38px;">1 – 65</td>
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<td class="text-center" style="width: 636px; height: 38px;" colspan="2">Multiple orifice hydrodynamic pore</td>
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<td class="text-center" style="width: 636px; height: 32px;" colspan="2">State-of-the-art electronics<br />Large memory for new features<br />Extensive fine-tuning options<br />Robust titanium pins</td>
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'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2>
<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2>
<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<div style="padding: 30px; background-color: #dad6e3;">
<p style="font-size: 1.2em; color: #2b2967;"><strong>Discover the NEW Megaruptor 3 HT</strong></p>
<p>The <strong>Megaruptor 3 HT</strong> offers an <strong>ultrafast protocol</strong> for increased sample processing speed to accommodate high-throughput sequencing projects. When using the ultrafast protocol, the <strong>Megaruptor 3 HT allows shearing of 96 samples in 2h into fragments of 7 to 40 kb</strong>.</p>
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<li><strong>Flexibility:</strong> Allows the fragment size range (within 7 to 40 kb) to be chosen for hands-free operation.</li>
<li><strong>Unmatched quality:</strong> Provides the tight DNA fragment distribution necessary for high quality long-read sequencing.</li>
<li><strong>Reliable:</strong> enables processing of highly viscous samples.</li>
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<center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center><center></center><center><iframe width="700" height="394" src="https://www.youtube.com/embed/VaWEK1vjbOY?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center>
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<th class="text-center" style="width: 296px; height: 55px;"></th>
<th class="text-center" style="width: 255px; height: 55px; background-color: #2b2967; color: white;">Megaruptor 3 regular</th>
<th class="text-center" style="width: 381px; height: 55px; background-color: #af3db2; color: white;">Megaruptor 3 HT with ultrafast protocol</th>
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<td class="text-center" style="width: 255px; height: 38px;">20 min – few hours</td>
<td class="text-center" style="width: 381px; height: 38px;">1 – 5 min</td>
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<td class="text-center" style="width: 255px; height: 38px;">0 – 150 ng/μl</td>
<td class="text-center" style="width: 381px; height: 38px;">1 – 50 ng/μl</td>
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<td class="text-center" style="width: 255px; height: 38px;">1 – 65</td>
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<td class="text-center" style="width: 381px; height: 56px;">96 samples in < 2h</td>
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<td class="text-center" style="width: 636px; height: 38px;" colspan="2">Multiple orifice hydrodynamic pore</td>
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<td class="text-center" style="width: 636px; height: 32px;" colspan="2">State-of-the-art electronics<br />Large memory for new features<br />Extensive fine-tuning options<br />Robust titanium pins</td>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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'format' => '16 samples',
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'type' => 'ACC',
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'price_EUR' => '370',
'price_USD' => '390',
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'price_JPY' => '57960',
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'slug' => 'megaruptor-3-shearing-kit',
'meta_title' => 'Megaruptor Shearing kit',
'meta_keywords' => '',
'meta_description' => 'Megaruptor Shearing kit',
'modified' => '2023-03-31 13:53:54',
'created' => '2019-03-07 12:37:06',
'ProductsGroup' => array(
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)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '1042',
'name' => 'Megaruptor® 3 ',
'description' => '<p class="p1"><strong>Your partner in long-read sequencing</strong></p>
<p class="p1">Designed to provide simple and automated <span class="s1">DNA sample preparation</span>, the Megaruptor gives state-ofthe-art shearing performance which is independent of the source, concentration, temperature, or salt content of a DNA sample, providing researcher with ultimate flexibility and ease of use. The shearing with the Megaruptor leads to optimal long-read sequencing using <span class="s1">PacBio</span><span class="s2">®</span>, and <span class="s1">Oxford Nanopore™ </span>technologies.</p>',
'image_id' => '186',
'type' => 'flyer',
'url' => 'files/flyers/megaruptor3_flyer.pdf',
'slug' => 'megaruptor3-flyer',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2019-03-05 14:23:36',
'created' => '2019-02-19 15:45:41',
'ProductsDocument' => array(
'id' => '3218',
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)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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