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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against NF-E2 (Cat. No. C15410240) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the HBE1 and GAPDH genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against NF-E2 (Cat. No. C15410240). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions of chromosome 11 and 12 surrounding the HB cluster and the GAPDH genes, respectively. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NF-E2</strong><br />Whole cell extracts from K562, THP-1 and HL-60 cells (lane 1, 2 and 3, respectively) were analysed by Western blot using the Diagenode antibody against NF-E2 (cat. No. C15410240) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small> <strong>Figure 4. Immunofluorescence with the Diagenode antibody directed against NF-E2</strong><br /> HepG2 cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against NF-E2 (Cat. C15410240) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against NF-E2 (Cat. No. C15410240) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the HBE1 and GAPDH genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against NF-E2 (Cat. No. C15410240). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions of chromosome 11 and 12 surrounding the HB cluster and the GAPDH genes, respectively. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NF-E2</strong><br />Whole cell extracts from K562, THP-1 and HL-60 cells (lane 1, 2 and 3, respectively) were analysed by Western blot using the Diagenode antibody against NF-E2 (cat. No. C15410240) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small> <strong>Figure 4. Immunofluorescence with the Diagenode antibody directed against NF-E2</strong><br /> HepG2 cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against NF-E2 (Cat. C15410240) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against NF-E2 (Cat. No. C15410240) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the HBE1 and GAPDH genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-12 columns"><center>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410240-chipseq-A.png" /></center><center>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410240-chipseq-B.png" /></center><center>C.<img src="https://www.diagenode.com/img/product/antibodies/C15410240-chipseq-C.png" /></center>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against NF-E2 (Cat. No. C15410240). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions of chromosome 11 and 12 surrounding the HB cluster and the GAPDH genes, respectively. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NF-E2</strong><br />Whole cell extracts from K562, THP-1 and HL-60 cells (lane 1, 2 and 3, respectively) were analysed by Western blot using the Diagenode antibody against NF-E2 (cat. No. C15410240) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small> <strong>Figure 4. Immunofluorescence with the Diagenode antibody directed against NF-E2</strong><br /> HepG2 cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against NF-E2 (Cat. C15410240) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against NF-E2 (Cat. No. C15410240) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the HBE1 and GAPDH genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against NF-E2 (Cat. No. C15410240). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions of chromosome 11 and 12 surrounding the HB cluster and the GAPDH genes, respectively. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NF-E2</strong><br /> Whole cell extracts from 293 cells (30 μg, figure 3A) or mouse spleen (50 μg, figure 3B) were analysed by Western blot using the Diagenode antibody against NF-E2 (Cat. No. C15410240) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small> <strong>Figure 4. Immunofluorescence with the Diagenode antibody directed against NF-E2</strong><br /> HepG2 cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against NF-E2 (Cat. C15410240) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against NF-E2 (Cat. No. C15410240) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the HBE1 and GAPDH genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against NF-E2 (Cat. No. C15410240). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions of chromosome 11 and 12 surrounding the HB cluster and the GAPDH genes, respectively. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NF-E2</strong><br />Whole cell extracts from K562, THP-1 and HL-60 cells (lane 1, 2 and 3, respectively) were analysed by Western blot using the Diagenode antibody against NF-E2 (cat. No. C15410240) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small> <strong>Figure 4. Immunofluorescence with the Diagenode antibody directed against NF-E2</strong><br /> HepG2 cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against NF-E2 (Cat. C15410240) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against NF-E2 (Cat. No. C15410240) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the HBE1 and GAPDH genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against NF-E2 (Cat. No. C15410240). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions of chromosome 11 and 12 surrounding the HB cluster and the GAPDH genes, respectively. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NF-E2</strong><br />Whole cell extracts from K562, THP-1 and HL-60 cells (lane 1, 2 and 3, respectively) were analysed by Western blot using the Diagenode antibody against NF-E2 (cat. No. C15410240) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small> <strong>Figure 4. Immunofluorescence with the Diagenode antibody directed against NF-E2</strong><br /> HepG2 cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against NF-E2 (Cat. C15410240) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against NF-E2 (Cat. No. C15410240). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions of chromosome 11 and 12 surrounding the HB cluster and the GAPDH genes, respectively. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NF-E2</strong><br />Whole cell extracts from K562, THP-1 and HL-60 cells (lane 1, 2 and 3, respectively) were analysed by Western blot using the Diagenode antibody against NF-E2 (cat. No. C15410240) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small> <strong>Figure 4. Immunofluorescence with the Diagenode antibody directed against NF-E2</strong><br /> HepG2 cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against NF-E2 (Cat. C15410240) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against NF-E2 (Cat. No. C15410240) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the HBE1 and GAPDH genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against NF-E2 (Cat. No. C15410240). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions of chromosome 11 and 12 surrounding the HB cluster and the GAPDH genes, respectively. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NF-E2</strong><br /> Whole cell extracts from 293 cells (30 μg, figure 3A) or mouse spleen (50 μg, figure 3B) were analysed by Western blot using the Diagenode antibody against NF-E2 (Cat. No. C15410240) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small> <strong>Figure 4. Immunofluorescence with the Diagenode antibody directed against NF-E2</strong><br /> HepG2 cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against NF-E2 (Cat. C15410240) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against NF-E2 (Cat. No. C15410240) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the HBE1 and GAPDH genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NF-E2</strong><br />Whole cell extracts from K562, THP-1 and HL-60 cells (lane 1, 2 and 3, respectively) were analysed by Western blot using the Diagenode antibody against NF-E2 (cat. No. C15410240) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small> <strong>Figure 4. Immunofluorescence with the Diagenode antibody directed against NF-E2</strong><br /> HepG2 cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against NF-E2 (Cat. C15410240) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><small> <strong>Figure 4. Immunofluorescence with the Diagenode antibody directed against NF-E2</strong><br /> HepG2 cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against NF-E2 (Cat. C15410240) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NF-E2</strong><br />Whole cell extracts from K562, THP-1 and HL-60 cells (lane 1, 2 and 3, respectively) were analysed by Western blot using the Diagenode antibody against NF-E2 (cat. No. C15410240) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small> <strong>Figure 4. Immunofluorescence with the Diagenode antibody directed against NF-E2</strong><br /> HepG2 cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against NF-E2 (Cat. C15410240) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against NF-E2 (Cat. No. C15410240) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the HBE1 and GAPDH genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against NF-E2 (Cat. No. C15410240). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions of chromosome 11 and 12 surrounding the HB cluster and the GAPDH genes, respectively. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NF-E2</strong><br /> Whole cell extracts from 293 cells (30 μg, figure 3A) or mouse spleen (50 μg, figure 3B) were analysed by Western blot using the Diagenode antibody against NF-E2 (Cat. No. C15410240) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small> <strong>Figure 4. Immunofluorescence with the Diagenode antibody directed against NF-E2</strong><br /> HepG2 cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against NF-E2 (Cat. C15410240) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NF-E2</strong><br />Whole cell extracts from K562, THP-1 and HL-60 cells (lane 1, 2 and 3, respectively) were analysed by Western blot using the Diagenode antibody against NF-E2 (cat. No. C15410240) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small> <strong>Figure 4. Immunofluorescence with the Diagenode antibody directed against NF-E2</strong><br /> HepG2 cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against NF-E2 (Cat. C15410240) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><small> <strong>Figure 4. Immunofluorescence with the Diagenode antibody directed against NF-E2</strong><br /> HepG2 cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against NF-E2 (Cat. C15410240) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against NF-E2 (Cat. No. C15410240) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the HBE1 and GAPDH genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NF-E2</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against NF-E2 (Cat. No. C15410240). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions of chromosome 11 and 12 surrounding the HB cluster and the GAPDH genes, respectively. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NF-E2</strong><br /> Whole cell extracts from 293 cells (30 μg, figure 3A) or mouse spleen (50 μg, figure 3B) were analysed by Western blot using the Diagenode antibody against NF-E2 (Cat. No. C15410240) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small> <strong>Figure 4. Immunofluorescence with the Diagenode antibody directed against NF-E2</strong><br /> HepG2 cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against NF-E2 (Cat. C15410240) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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