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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
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<td>Fig 1, 2</td>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>5 μg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:1,000 - 1:10,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.</small></p>',
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'description' => '<p>Polyclonal antibody raised in rabbit against <strong>OCT4 (Octamer-Binding Protein 4)</strong>, using 4 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.</p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chip.png" alt="OCT4 Antibody ChIP Grade" caption="false" width="288" height="209" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig4.png" alt="OCT4 Antibody validated in ChIP-seq" caption="false" width="288" height="57" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>5 μg/ChIP</td>
<td>Fig 1, 2</td>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-4 columns">
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'locale' => 'zho'
)
$description = ''
$name = 'ChIP-qPCR (ab)'
$document = array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
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'id' => '1442',
'product_id' => '2424',
'document_id' => '38'
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'id' => '2316',
'name' => 'OCT4 Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2022-08-29 11:57:21',
'created' => '2022-08-29 11:57:21',
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'product_id' => '2424',
'safety_sheet_id' => '2316'
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$publication = array(
'id' => '2959',
'name' => 'Reversion to an embryonic alternative splicing program enhances leukemia stem cell self-renewal.',
'authors' => 'Holm F et al.',
'description' => '<p>Formative research suggests that a human embryonic stem cell-specific alternative splicing gene regulatory network, which is repressed by Muscleblind-like (MBNL) RNA binding proteins, is involved in cell reprogramming. In this study, RNA sequencing, splice isoform-specific quantitative RT-PCR, lentiviral transduction, and in vivo humanized mouse model studies demonstrated that malignant reprogramming of progenitors into self-renewing blast crisis chronic myeloid leukemia stem cells (BC LSCs) was partially driven by decreased MBNL3. Lentiviral knockdown of MBNL3 resulted in reversion to an embryonic alternative splice isoform program typified by overexpression of CD44 transcript variant 3, containing variant exons 8-10, and BC LSC proliferation. Although isoform-specific lentiviral CD44v3 overexpression enhanced chronic phase chronic myeloid leukemia (CML) progenitor replating capacity, lentiviral shRNA knockdown abrogated these effects. Combined treatment with a humanized pan-CD44 monoclonal antibody and a breakpoint cluster region - ABL proto-oncogene 1, nonreceptor tyrosine kinase (BCR-ABL1) antagonist inhibited LSC maintenance in a niche-dependent manner. In summary, MBNL3 down-regulation-related reversion to an embryonic alternative splicing program, typified by CD44v3 overexpression, represents a previously unidentified mechanism governing malignant progenitor reprogramming in malignant microenvironments and provides a pivotal opportunity for selective BC LSC detection and therapeutic elimination.</p>',
'date' => '2015-12-15',
'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26621726',
'doi' => ' 10.1073/pnas.1506943112',
'modified' => '2016-06-23 10:25:24',
'created' => '2016-06-23 10:25:24',
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'id' => '1368',
'product_id' => '2424',
'publication_id' => '2959'
)
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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