ELISA Enzyme-linked immunosorbent assay. Read more |
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Lot | A1036-001 |
---|---|
Concentration | not determined |
Species reactivity | Human |
Type | Polyclonal |
Purity | Whole antiserum |
Host | Rabbit |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Applications | Suggested dilution | References |
---|---|---|
ELISA | 1:1,000 | Fig 1 |
* The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:1,000 dilution.
Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against PIWIL1 (cat. No. CS-PA024-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:23,800.
Datasheet PIWIL1 CS-PA024-100 DATASHEET Datasheet description | Download |
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
How to properly cite this product in your workDiagenode strongly recommends using this: PIWIL1 polyclonal antibody (Diagenode Cat# C15310244 Lot# A1036-001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. PIWIL1 probably is a component of some RISC complex, which mediates RNA cleavage and translational silencing. It also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability.', 'clonality' => '', 'isotype' => '', 'lot' => 'A1036-001', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:1,000</td> <td>Fig 1</td> </tr> </tbody> </table> <p><small><sup>*</sup> The optimal dilution for other applications should be determined by the end user. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against PIWIL1 (cat. No. CS-PA024-100). The plates were coated with the peptides used for immunization of the rabbit. 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Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. PIWIL1 probably is a component of some RISC complex, which mediates RNA cleavage and translational silencing. It also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability.', 'clonality' => '', 'isotype' => '', 'lot' => 'A1036-001', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:1,000</td> <td>Fig 1</td> </tr> </tbody> </table> <p><small><sup>*</sup> The optimal dilution for other applications should be determined by the end user. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against PIWIL1 (cat. No. CS-PA024-100). The plates were coated with the peptides used for immunization of the rabbit. 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$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'cn', 'meta_keywords' => '', 'meta_description' => 'PIWIL1 (Piwi-like 1) Polyclonal Antibody validated in ELISA. Batch-specific data available on the website. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against PIWIL1 (cat. No. CS-PA024-100). The plates were coated with the peptides used for immunization of the rabbit. 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Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. PIWIL1 probably is a component of some RISC complex, which mediates RNA cleavage and translational silencing. It also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability.', 'clonality' => '', 'isotype' => '', 'lot' => 'A1036-001', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:1,000</td> <td>Fig 1</td> </tr> </tbody> </table> <p><small><sup>*</sup> The optimal dilution for other applications should be determined by the end user. 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Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. PIWIL1 probably is a component of some RISC complex, which mediates RNA cleavage and translational silencing. It also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability.', 'clonality' => '', 'isotype' => '', 'lot' => 'A1036-001', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:1,000</td> <td>Fig 1</td> </tr> </tbody> </table> <p><small><sup>*</sup> The optimal dilution for other applications should be determined by the end user. 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These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div> <p></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'mirna-sirna', 'cookies_tag_id' => null, 'meta_keywords' => 'miRNA & siRNA meta Polyclonal antibody,Monoclonal antibody', 'meta_description' => 'Diagenode offers Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing', 'meta_title' => 'Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing | Diagenode', 'modified' => '2020-09-01 11:42:06', 'created' => '2014-11-12 02:34:56', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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