PspCas13b monoclonal antibody (sample size)

Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]
Code Context
<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'meta_keywords' => '',
	'meta_description' => 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available',
	'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
	'product' => array(
		'Product' => array(
			'id' => '3016',
			'antibody_id' => '646',
			'name' => 'PspCas13b monoclonal antibody (sample size)',
			'description' => '',
			'label1' => 'Validation data',
			'info1' => '',
			'label2' => '',
			'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig1-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig2-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
			'label3' => '',
			'info3' => '',
			'format' => '10 μg',
			'catalog_number' => 'C15200250-10',
			'old_catalog_number' => 'C15200244-10',
			'sf_code' => 'C15200250-D001-000582',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '125',
			'price_USD' => '130',
			'price_GBP' => '120',
			'price_JPY' => '19580',
			'price_CNY' => '0',
			'price_AUD' => '325',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => false,
			'last_datasheet_update' => '',
			'slug' => 'pspcas13b-monoclonal-antibody-10-ug',
			'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available',
			'modified' => '2022-01-05 15:56:57',
			'created' => '2019-02-13 17:27:06',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '646',
			'name' => 'PspCas13b monoclonal antibody (sample size)',
			'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.',
			'clonality' => '',
			'isotype' => 'IgG2a',
			'lot' => '001',
			'concentration' => '1 µg/µl',
			'reactivity' => 'Prevotella sp.',
			'type' => 'Monoclonal',
			'purity' => 'Protein G purified monoclonal antibody.',
			'classification' => '',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western blotting</td>
<td>1:1,000 &ndash; 1:4,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
			'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
			'storage_buffer' => 'TBS containing 0.02 % Na-azide.',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => 'pspcas13b-antibody-10-ug',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2021-07-29 14:11:09',
			'created' => '2021-07-29 14:11:09',
			'select_label' => '646 - PspCas13b monoclonal antibody (sample size) (001 - 1 µg/µl - Prevotella sp. - Protein G purified monoclonal antibody. - Mouse)'
		),
		'Slave' => array(),
		'Group' => array(
			'Group' => array(
				[maximum depth reached]
			),
			'Master' => array(
				[maximum depth reached]
			),
			'Product' => array(
				[maximum depth reached]
			)
		),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	),
	'meta_canonical' => 'https://www.diagenode.com/cn/p/pspcas13b-monoclonal-antibody-50-ul'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$meta_keywords = ''
$meta_description = 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available'
$meta_title = 'PspCas13b Monoclonal Antibody | Diagenode'
$product = array(
	'Product' => array(
		'id' => '3016',
		'antibody_id' => '646',
		'name' => 'PspCas13b monoclonal antibody (sample size)',
		'description' => '<p>Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13b) using a recombinant protein.</p>',
		'label1' => 'Validation data',
		'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
		'label2' => 'Validation data',
		'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig1-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig2-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
		'label3' => '',
		'info3' => '',
		'format' => '10 μg',
		'catalog_number' => 'C15200250-10',
		'old_catalog_number' => 'C15200244-10',
		'sf_code' => 'C15200250-D001-000582',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '125',
		'price_USD' => '130',
		'price_GBP' => '120',
		'price_JPY' => '19580',
		'price_CNY' => '0',
		'price_AUD' => '325',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'pspcas13b-monoclonal-antibody-10-ug',
		'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available',
		'modified' => '2022-01-05 15:56:57',
		'created' => '2019-02-13 17:27:06',
		'locale' => 'zho'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => '646',
		'name' => 'PspCas13b monoclonal antibody (sample size)',
		'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.',
		'clonality' => '',
		'isotype' => 'IgG2a',
		'lot' => '001',
		'concentration' => '1 µg/µl',
		'reactivity' => 'Prevotella sp.',
		'type' => 'Monoclonal',
		'purity' => 'Protein G purified monoclonal antibody.',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western blotting</td>
<td>1:1,000 &ndash; 1:4,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
		'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
		'storage_buffer' => 'TBS containing 0.02 % Na-azide.',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => 'pspcas13b-antibody-10-ug',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2021-07-29 14:11:09',
		'created' => '2021-07-29 14:11:09',
		'select_label' => '646 - PspCas13b monoclonal antibody (sample size) (001 - 1 µg/µl - Prevotella sp. - Protein G purified monoclonal antibody. - Mouse)'
	),
	'Slave' => array(),
	'Group' => array(
		'Group' => array(
			'id' => '258',
			'name' => 'C15200244',
			'product_id' => '3011',
			'modified' => '2019-03-19 13:37:19',
			'created' => '2019-03-19 13:37:19'
		),
		'Master' => array(
			'id' => '3011',
			'antibody_id' => '549',
			'name' => 'PspCas13b Antibody',
			'description' => '<p>Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13b) using a recombinant protein.</p>',
			'label1' => 'Target description',
			'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
			'label2' => 'Validation data',
			'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig1-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig2-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
			'label3' => '',
			'info3' => '',
			'format' => '50 µg',
			'catalog_number' => 'C15200250',
			'old_catalog_number' => 'C15200244',
			'sf_code' => 'C15200250-D001-000581',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '445',
			'price_USD' => '465',
			'price_GBP' => '420',
			'price_JPY' => '69710',
			'price_CNY' => '0',
			'price_AUD' => '1162',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => 'December 13, 2019',
			'slug' => 'pspcas13b-monoclonal-antibody-50-ul',
			'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'PspCas13b (CRISPR associated protein 13b from Prevotella sp.) Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
			'modified' => '2022-01-05 15:56:43',
			'created' => '2019-02-13 15:10:44'
		),
		'Product' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		)
	),
	'Related' => array(),
	'Application' => array(
		(int) 0 => array(
			'id' => '19',
			'position' => '10',
			'parent_id' => '40',
			'name' => 'WB',
			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
			'in_footer' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'slug' => 'western-blot-antibodies',
			'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
			'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
			'modified' => '2016-04-26 12:44:51',
			'created' => '2015-01-07 09:20:00',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '103',
			'position' => '0',
			'parent_id' => '4',
			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'all-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
			'modified' => '2019-07-03 10:55:44',
			'created' => '2015-11-02 14:49:22',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		),
		(int) 1 => array(
			'id' => '100',
			'position' => '41',
			'parent_id' => '4',
			'name' => 'CRISPR/Cas9 antibodies',
			'description' => '<p>The <strong>CRISPR/Cas genome editing system</strong> uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, this technology, can also be used to mediate up- or downregulation of specific endogenous genes or to alter histone modifications or DNA methylation. The best characterized CRISPR-associated nucleases are the <strong>Cas9 proteins</strong> from <em>Streptococcus pyogenes</em> and <em>Staphylococcus aureus</em>. Recently two new CRISPR-associated nucleases, named <strong>Cpf1</strong> (from <em>Lachnospiraceae bacterium</em> and <em>Acidaminococcus sp.</em>), have been described.<br /><br /><span>Diagenode, a dedicated supplier of high quality Cas9 antibodies, was the first company that offered the antibody Cas9 (clone 7A9). </span><span>Diagenode now offers the largest collection of optimized anti-CRISPR/Cas antibodies, validated in a number of different applications including Western blot (WB), immunofluorescence (IF), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP).</span></p>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3><a name="4reasons"></a>Four critical reasons to use anti-CRISPR/Cas9 antibodies</h3>
<p>CRISPR/Cas9 genome editing allows for double-stranded DNA breaks at specific sequences to efficiently disrupt, excise, mutate, insert, or replace genes. The precision of transfection and the level of Cas9 expression should be controlled during the editing processes using specific anti-CRISPR/Cas9 antibodies.</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-1.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #1</strong> Check the transfection success of the Cas9 protein</h4>
<p>Check <strong>Cas9 expression level</strong> using <strong>Western blot</strong> with validated anti-Cas9 antibodies.</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-2.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #2</strong> Check that the Cas9 protein was delivered to the nucleus</h4>
<p>Determine the nuclear localization of Cas9 protein via <strong>IF</strong> or <strong>IHC</strong> using validated anti-Cas9 antibodies</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-3.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #3</strong> Check the Cas9 expression level</h4>
<p><strong>Transient systems</strong>: Check that <strong>Cas9 expression</strong> was transient by using <strong>Western blot</strong> and anti-Cas9 antibodies. Prolonged Cas9 expression will lead to more off-target mutations.</p>
<p><strong>Stable transformants</strong>: Isolate several clones and screen to check the level of <strong>Cas9 expression</strong> using <strong>Western blot</strong> and specific anti-Cas9 antibodies. High level of Cas9 expression can lead to non-specific activity.</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-4.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #4</strong> Check the binding specificity of Cas9</h4>
<p>Test the binding specificity with an sgRNA of choice by using the <strong>ChIP-grade anti-Cas9 antibody</strong> and the primers for targeted and non-targeted region to see if Cas9 is bound to the correct region.</p>
<p>For more details go to the page <strong><a href="https://www.diagenode.com/en/categories/selection-of-guide-rna-by-ChIP">Selection of guide RNA by ChIP</a></strong>.</p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>Published examples of the use of anti-Cas9 antibodies:</h3>
<ul>
<li><a href="../publications/view/2823">A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing</a></li>
<li><a href="../publications/view/2792">A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage</a></li>
<li><a href="../publications/view/2765">Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection</a></li>
<li><a href="../publications/view/2679">Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells</a></li>
<li><a href="../publications/view/2645">Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis</a></li>
<li><a href="../publications/view/2961">An inducible lentiviral guide RNA platform enables the identification of tumor-essential genes and tumor-promoting mutations in vivo</a></li>
</ul>
<h5><strong>Diagenode offers the largest collection of validated anti-CRISPR/Cas antibodies, validated in different applications (WB, IF, IP and ChIP).</strong></h5>
</div>
</div>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'crispr-cas9',
			'cookies_tag_id' => '1',
			'meta_keywords' => 'crispr cas9 antibodies,cas9 applications,cas9 protocol,cas9 review',
			'meta_description' => 'Diagenode offers a broad range of highly validated CRISPR/Cas9 Antibodies, Recommended for different applications (WB, IF, IP and chromatin IP). These Antibodies have been raised against the N- or C-terminus of the Cas9 Nuclease.',
			'meta_title' => 'Best CRISPR-Cas9 Antibodies on the Market | Diagenode',
			'modified' => '2020-08-03 15:29:26',
			'created' => '2015-10-19 14:55:29',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '134',
			'position' => '0',
			'parent_id' => '100',
			'name' => 'RNA-targeting CRISPR antibodies',
			'description' => '<p>The latest tool for CRISPR gene editing is a new CRISPR-C2C2 (Cas13a) system which utilizes RNA-guided RNA-targeting for RNA knockdown and binding in cells. C2C2 (Cas13a) may be useful for isolating specific RNA sequences or even studying single RNA molecules with high specificity. Unlike editing DNA as with Cas9 which makes permanent genomic changes,&nbsp; targeting RNA with C2C2 allows temporarily altering the amount of protein potentially offering a better therapeutic approach in the future.&nbsp;</p>
<p>Use Diagenode's new C2C2 (Cas13a) polyclonal antibody to determine precision and success metrics in CRISPR experiments.</p>',
			'no_promo' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'antibodies-against-rna-targeting-crispr',
			'cookies_tag_id' => '1',
			'meta_keywords' => '',
			'meta_description' => '',
			'meta_title' => 'Antibodies against RNA-targeting CRISPR',
			'modified' => '2018-04-26 15:44:54',
			'created' => '2018-03-05 11:46:15',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '102',
			'position' => '1',
			'parent_id' => '4',
			'name' => 'Sample size antibodies',
			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => true,
			'is_antibody' => true,
			'slug' => 'sample-size-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
			'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
			'meta_title' => 'Sample-size Antibodies | Diagenode',
			'modified' => '2019-07-03 10:57:05',
			'created' => '2015-10-27 12:13:34',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '1141',
			'name' => 'PspCas13b monoclonal antibody (sample size)',
			'description' => '',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/antibodies/Datasheet-pspcas13b-10ul-lot001.pdf',
			'slug' => 'pspcas13b-monoclonal-antibody-sample-datasheet',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2021-07-29 14:21:12',
			'created' => '2021-07-29 14:20:37',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '250',
			'name' => 'product/antibodies/antibody.png',
			'alt' => 'Mouse IgG',
			'modified' => '2020-11-27 07:00:09',
			'created' => '2015-07-17 10:12:18',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '1606',
			'name' => 'PspCas13b antibody SDS GB en',
			'language' => 'en',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-GB-en-GHS_2_0.pdf',
			'countries' => 'GB',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1608',
			'name' => 'PspCas13b antibody SDS US en',
			'language' => 'en',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-US-en-GHS_2_0.pdf',
			'countries' => 'US',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1603',
			'name' => 'PspCas13b antibody SDS DE de',
			'language' => 'de',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-DE-de-GHS_2_0.pdf',
			'countries' => 'DE',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1607',
			'name' => 'PspCas13b antibody SDS JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-JP-ja-GHS_3_0.pdf',
			'countries' => 'JP',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '1602',
			'name' => 'PspCas13b antibody SDS BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-BE-nl-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '1601',
			'name' => 'PspCas13b antibody SDS BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-BE-fr-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '1605',
			'name' => 'PspCas13b antibody SDS FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-FR-fr-GHS_2_0.pdf',
			'countries' => 'FR',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '1604',
			'name' => 'PspCas13b antibody SDS ES es',
			'language' => 'es',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-ES-es-GHS_2_0.pdf',
			'countries' => 'ES',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/pspcas13b-monoclonal-antibody-50-ul'
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
	(int) 0 => array(
		'id' => '3011',
		'antibody_id' => '549',
		'name' => 'PspCas13b Antibody',
		'description' => '<p>Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13b) using a recombinant protein.</p>',
		'label1' => 'Target description',
		'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
		'label2' => 'Validation data',
		'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig1-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig2-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
		'label3' => '',
		'info3' => '',
		'format' => '50 µg',
		'catalog_number' => 'C15200250',
		'old_catalog_number' => 'C15200244',
		'sf_code' => 'C15200250-D001-000581',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '445',
		'price_USD' => '465',
		'price_GBP' => '420',
		'price_JPY' => '69710',
		'price_CNY' => '0',
		'price_AUD' => '1162',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => 'December 13, 2019',
		'slug' => 'pspcas13b-monoclonal-antibody-50-ul',
		'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'PspCas13b (CRISPR associated protein 13b from Prevotella sp.) Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
		'modified' => '2022-01-05 15:56:43',
		'created' => '2019-02-13 15:10:44'
	)
)
$pro = array(
	'id' => '3016',
	'antibody_id' => '646',
	'name' => 'PspCas13b Antibody (sample size)',
	'description' => '',
	'label1' => 'Target description',
	'info1' => '',
	'label2' => 'Validation data',
	'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig1-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig2-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
	'label3' => '',
	'info3' => '',
	'format' => '10 μg',
	'catalog_number' => 'C15200250-10',
	'old_catalog_number' => 'C15200244-10',
	'sf_code' => 'C15200250-D001-000582',
	'type' => 'FRE',
	'search_order' => '03-Antibody',
	'price_EUR' => '125',
	'price_USD' => '130',
	'price_GBP' => '120',
	'price_JPY' => '19580',
	'price_CNY' => '0',
	'price_AUD' => '325',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '',
	'slug' => 'pspcas13b-monoclonal-antibody-10-ug',
	'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
	'meta_keywords' => '',
	'meta_description' => 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available',
	'modified' => '2022-01-05 15:56:57',
	'created' => '2019-02-13 17:27:06',
	'ProductsGroup' => array(
		'id' => '293',
		'product_id' => '3016',
		'group_id' => '258'
	)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ' <span style="color:#CCC">(C15200244)</span>'
$country_code = 'US'
$other_format = array(
	'id' => '3011',
	'antibody_id' => '549',
	'name' => 'PspCas13b Antibody',
	'description' => '<p>Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13b) using a recombinant protein.</p>',
	'label1' => 'Target description',
	'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
	'label2' => 'Validation data',
	'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig1-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig2-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
	'label3' => '',
	'info3' => '',
	'format' => '50 µg',
	'catalog_number' => 'C15200250',
	'old_catalog_number' => 'C15200244',
	'sf_code' => 'C15200250-D001-000581',
	'type' => 'FRE',
	'search_order' => '03-Antibody',
	'price_EUR' => '445',
	'price_USD' => '465',
	'price_GBP' => '420',
	'price_JPY' => '69710',
	'price_CNY' => '0',
	'price_AUD' => '1162',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => 'December 13, 2019',
	'slug' => 'pspcas13b-monoclonal-antibody-50-ul',
	'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
	'meta_keywords' => '',
	'meta_description' => 'PspCas13b (CRISPR associated protein 13b from Prevotella sp.) Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
	'modified' => '2022-01-05 15:56:43',
	'created' => '2019-02-13 15:10:44'
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'ProductsApplication' => array(
		'id' => '5012',
		'product_id' => '3016',
		'application_id' => '19'
	)
)
$slugs = array(
	(int) 0 => 'western-blot-antibodies'
)
$applications = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'locale' => 'zho'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
	'id' => '1141',
	'name' => 'PspCas13b monoclonal antibody (sample size)',
	'description' => '',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/antibodies/Datasheet-pspcas13b-10ul-lot001.pdf',
	'slug' => 'pspcas13b-monoclonal-antibody-sample-datasheet',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2021-07-29 14:21:12',
	'created' => '2021-07-29 14:20:37',
	'ProductsDocument' => array(
		'id' => '3136',
		'product_id' => '3016',
		'document_id' => '1141'
	)
)
$sds = array(
	'id' => '1604',
	'name' => 'PspCas13b antibody SDS ES es',
	'language' => 'es',
	'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-ES-es-GHS_2_0.pdf',
	'countries' => 'ES',
	'modified' => '2021-08-31 09:32:37',
	'created' => '2021-08-31 09:32:37',
	'ProductsSafetySheet' => array(
		'id' => '2799',
		'product_id' => '3016',
		'safety_sheet_id' => '1604'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]
Code Context
<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'meta_keywords' => '',
	'meta_description' => 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available',
	'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
	'product' => array(
		'Product' => array(
			'id' => '3016',
			'antibody_id' => '646',
			'name' => 'PspCas13b monoclonal antibody (sample size)',
			'description' => '',
			'label1' => 'Validation data',
			'info1' => '',
			'label2' => '',
			'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig1-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig2-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
			'label3' => '',
			'info3' => '',
			'format' => '10 μg',
			'catalog_number' => 'C15200250-10',
			'old_catalog_number' => 'C15200244-10',
			'sf_code' => 'C15200250-D001-000582',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '125',
			'price_USD' => '130',
			'price_GBP' => '120',
			'price_JPY' => '19580',
			'price_CNY' => '0',
			'price_AUD' => '325',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => false,
			'last_datasheet_update' => '',
			'slug' => 'pspcas13b-monoclonal-antibody-10-ug',
			'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available',
			'modified' => '2022-01-05 15:56:57',
			'created' => '2019-02-13 17:27:06',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '646',
			'name' => 'PspCas13b monoclonal antibody (sample size)',
			'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.',
			'clonality' => '',
			'isotype' => 'IgG2a',
			'lot' => '001',
			'concentration' => '1 µg/µl',
			'reactivity' => 'Prevotella sp.',
			'type' => 'Monoclonal',
			'purity' => 'Protein G purified monoclonal antibody.',
			'classification' => '',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western blotting</td>
<td>1:1,000 &ndash; 1:4,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
			'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
			'storage_buffer' => 'TBS containing 0.02 % Na-azide.',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => 'pspcas13b-antibody-10-ug',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2021-07-29 14:11:09',
			'created' => '2021-07-29 14:11:09',
			'select_label' => '646 - PspCas13b monoclonal antibody (sample size) (001 - 1 µg/µl - Prevotella sp. - Protein G purified monoclonal antibody. - Mouse)'
		),
		'Slave' => array(),
		'Group' => array(
			'Group' => array(
				[maximum depth reached]
			),
			'Master' => array(
				[maximum depth reached]
			),
			'Product' => array(
				[maximum depth reached]
			)
		),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	),
	'meta_canonical' => 'https://www.diagenode.com/cn/p/pspcas13b-monoclonal-antibody-50-ul'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$meta_keywords = ''
$meta_description = 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available'
$meta_title = 'PspCas13b Monoclonal Antibody | Diagenode'
$product = array(
	'Product' => array(
		'id' => '3016',
		'antibody_id' => '646',
		'name' => 'PspCas13b monoclonal antibody (sample size)',
		'description' => '<p>Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13b) using a recombinant protein.</p>',
		'label1' => 'Validation data',
		'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
		'label2' => 'Validation data',
		'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig1-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig2-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
		'label3' => '',
		'info3' => '',
		'format' => '10 μg',
		'catalog_number' => 'C15200250-10',
		'old_catalog_number' => 'C15200244-10',
		'sf_code' => 'C15200250-D001-000582',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '125',
		'price_USD' => '130',
		'price_GBP' => '120',
		'price_JPY' => '19580',
		'price_CNY' => '0',
		'price_AUD' => '325',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'pspcas13b-monoclonal-antibody-10-ug',
		'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available',
		'modified' => '2022-01-05 15:56:57',
		'created' => '2019-02-13 17:27:06',
		'locale' => 'zho'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => '646',
		'name' => 'PspCas13b monoclonal antibody (sample size)',
		'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.',
		'clonality' => '',
		'isotype' => 'IgG2a',
		'lot' => '001',
		'concentration' => '1 µg/µl',
		'reactivity' => 'Prevotella sp.',
		'type' => 'Monoclonal',
		'purity' => 'Protein G purified monoclonal antibody.',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western blotting</td>
<td>1:1,000 &ndash; 1:4,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
		'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
		'storage_buffer' => 'TBS containing 0.02 % Na-azide.',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => 'pspcas13b-antibody-10-ug',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2021-07-29 14:11:09',
		'created' => '2021-07-29 14:11:09',
		'select_label' => '646 - PspCas13b monoclonal antibody (sample size) (001 - 1 µg/µl - Prevotella sp. - Protein G purified monoclonal antibody. - Mouse)'
	),
	'Slave' => array(),
	'Group' => array(
		'Group' => array(
			'id' => '258',
			'name' => 'C15200244',
			'product_id' => '3011',
			'modified' => '2019-03-19 13:37:19',
			'created' => '2019-03-19 13:37:19'
		),
		'Master' => array(
			'id' => '3011',
			'antibody_id' => '549',
			'name' => 'PspCas13b Antibody',
			'description' => '<p>Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13b) using a recombinant protein.</p>',
			'label1' => 'Target description',
			'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
			'label2' => 'Validation data',
			'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig1-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig2-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
			'label3' => '',
			'info3' => '',
			'format' => '50 µg',
			'catalog_number' => 'C15200250',
			'old_catalog_number' => 'C15200244',
			'sf_code' => 'C15200250-D001-000581',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '445',
			'price_USD' => '465',
			'price_GBP' => '420',
			'price_JPY' => '69710',
			'price_CNY' => '0',
			'price_AUD' => '1162',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => 'December 13, 2019',
			'slug' => 'pspcas13b-monoclonal-antibody-50-ul',
			'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'PspCas13b (CRISPR associated protein 13b from Prevotella sp.) Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
			'modified' => '2022-01-05 15:56:43',
			'created' => '2019-02-13 15:10:44'
		),
		'Product' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		)
	),
	'Related' => array(),
	'Application' => array(
		(int) 0 => array(
			'id' => '19',
			'position' => '10',
			'parent_id' => '40',
			'name' => 'WB',
			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
			'in_footer' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'slug' => 'western-blot-antibodies',
			'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
			'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
			'modified' => '2016-04-26 12:44:51',
			'created' => '2015-01-07 09:20:00',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '103',
			'position' => '0',
			'parent_id' => '4',
			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'all-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
			'modified' => '2019-07-03 10:55:44',
			'created' => '2015-11-02 14:49:22',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		),
		(int) 1 => array(
			'id' => '100',
			'position' => '41',
			'parent_id' => '4',
			'name' => 'CRISPR/Cas9 antibodies',
			'description' => '<p>The <strong>CRISPR/Cas genome editing system</strong> uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, this technology, can also be used to mediate up- or downregulation of specific endogenous genes or to alter histone modifications or DNA methylation. The best characterized CRISPR-associated nucleases are the <strong>Cas9 proteins</strong> from <em>Streptococcus pyogenes</em> and <em>Staphylococcus aureus</em>. Recently two new CRISPR-associated nucleases, named <strong>Cpf1</strong> (from <em>Lachnospiraceae bacterium</em> and <em>Acidaminococcus sp.</em>), have been described.<br /><br /><span>Diagenode, a dedicated supplier of high quality Cas9 antibodies, was the first company that offered the antibody Cas9 (clone 7A9). </span><span>Diagenode now offers the largest collection of optimized anti-CRISPR/Cas antibodies, validated in a number of different applications including Western blot (WB), immunofluorescence (IF), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP).</span></p>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3><a name="4reasons"></a>Four critical reasons to use anti-CRISPR/Cas9 antibodies</h3>
<p>CRISPR/Cas9 genome editing allows for double-stranded DNA breaks at specific sequences to efficiently disrupt, excise, mutate, insert, or replace genes. The precision of transfection and the level of Cas9 expression should be controlled during the editing processes using specific anti-CRISPR/Cas9 antibodies.</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-1.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #1</strong> Check the transfection success of the Cas9 protein</h4>
<p>Check <strong>Cas9 expression level</strong> using <strong>Western blot</strong> with validated anti-Cas9 antibodies.</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-2.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #2</strong> Check that the Cas9 protein was delivered to the nucleus</h4>
<p>Determine the nuclear localization of Cas9 protein via <strong>IF</strong> or <strong>IHC</strong> using validated anti-Cas9 antibodies</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-3.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #3</strong> Check the Cas9 expression level</h4>
<p><strong>Transient systems</strong>: Check that <strong>Cas9 expression</strong> was transient by using <strong>Western blot</strong> and anti-Cas9 antibodies. Prolonged Cas9 expression will lead to more off-target mutations.</p>
<p><strong>Stable transformants</strong>: Isolate several clones and screen to check the level of <strong>Cas9 expression</strong> using <strong>Western blot</strong> and specific anti-Cas9 antibodies. High level of Cas9 expression can lead to non-specific activity.</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-4.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #4</strong> Check the binding specificity of Cas9</h4>
<p>Test the binding specificity with an sgRNA of choice by using the <strong>ChIP-grade anti-Cas9 antibody</strong> and the primers for targeted and non-targeted region to see if Cas9 is bound to the correct region.</p>
<p>For more details go to the page <strong><a href="https://www.diagenode.com/en/categories/selection-of-guide-rna-by-ChIP">Selection of guide RNA by ChIP</a></strong>.</p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>Published examples of the use of anti-Cas9 antibodies:</h3>
<ul>
<li><a href="../publications/view/2823">A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing</a></li>
<li><a href="../publications/view/2792">A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage</a></li>
<li><a href="../publications/view/2765">Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection</a></li>
<li><a href="../publications/view/2679">Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells</a></li>
<li><a href="../publications/view/2645">Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis</a></li>
<li><a href="../publications/view/2961">An inducible lentiviral guide RNA platform enables the identification of tumor-essential genes and tumor-promoting mutations in vivo</a></li>
</ul>
<h5><strong>Diagenode offers the largest collection of validated anti-CRISPR/Cas antibodies, validated in different applications (WB, IF, IP and ChIP).</strong></h5>
</div>
</div>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'crispr-cas9',
			'cookies_tag_id' => '1',
			'meta_keywords' => 'crispr cas9 antibodies,cas9 applications,cas9 protocol,cas9 review',
			'meta_description' => 'Diagenode offers a broad range of highly validated CRISPR/Cas9 Antibodies, Recommended for different applications (WB, IF, IP and chromatin IP). These Antibodies have been raised against the N- or C-terminus of the Cas9 Nuclease.',
			'meta_title' => 'Best CRISPR-Cas9 Antibodies on the Market | Diagenode',
			'modified' => '2020-08-03 15:29:26',
			'created' => '2015-10-19 14:55:29',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '134',
			'position' => '0',
			'parent_id' => '100',
			'name' => 'RNA-targeting CRISPR antibodies',
			'description' => '<p>The latest tool for CRISPR gene editing is a new CRISPR-C2C2 (Cas13a) system which utilizes RNA-guided RNA-targeting for RNA knockdown and binding in cells. C2C2 (Cas13a) may be useful for isolating specific RNA sequences or even studying single RNA molecules with high specificity. Unlike editing DNA as with Cas9 which makes permanent genomic changes,&nbsp; targeting RNA with C2C2 allows temporarily altering the amount of protein potentially offering a better therapeutic approach in the future.&nbsp;</p>
<p>Use Diagenode's new C2C2 (Cas13a) polyclonal antibody to determine precision and success metrics in CRISPR experiments.</p>',
			'no_promo' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'antibodies-against-rna-targeting-crispr',
			'cookies_tag_id' => '1',
			'meta_keywords' => '',
			'meta_description' => '',
			'meta_title' => 'Antibodies against RNA-targeting CRISPR',
			'modified' => '2018-04-26 15:44:54',
			'created' => '2018-03-05 11:46:15',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '102',
			'position' => '1',
			'parent_id' => '4',
			'name' => 'Sample size antibodies',
			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => true,
			'is_antibody' => true,
			'slug' => 'sample-size-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
			'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
			'meta_title' => 'Sample-size Antibodies | Diagenode',
			'modified' => '2019-07-03 10:57:05',
			'created' => '2015-10-27 12:13:34',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '1141',
			'name' => 'PspCas13b monoclonal antibody (sample size)',
			'description' => '',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/antibodies/Datasheet-pspcas13b-10ul-lot001.pdf',
			'slug' => 'pspcas13b-monoclonal-antibody-sample-datasheet',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2021-07-29 14:21:12',
			'created' => '2021-07-29 14:20:37',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '250',
			'name' => 'product/antibodies/antibody.png',
			'alt' => 'Mouse IgG',
			'modified' => '2020-11-27 07:00:09',
			'created' => '2015-07-17 10:12:18',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '1606',
			'name' => 'PspCas13b antibody SDS GB en',
			'language' => 'en',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-GB-en-GHS_2_0.pdf',
			'countries' => 'GB',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1608',
			'name' => 'PspCas13b antibody SDS US en',
			'language' => 'en',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-US-en-GHS_2_0.pdf',
			'countries' => 'US',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1603',
			'name' => 'PspCas13b antibody SDS DE de',
			'language' => 'de',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-DE-de-GHS_2_0.pdf',
			'countries' => 'DE',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1607',
			'name' => 'PspCas13b antibody SDS JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-JP-ja-GHS_3_0.pdf',
			'countries' => 'JP',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '1602',
			'name' => 'PspCas13b antibody SDS BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-BE-nl-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '1601',
			'name' => 'PspCas13b antibody SDS BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-BE-fr-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '1605',
			'name' => 'PspCas13b antibody SDS FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-FR-fr-GHS_2_0.pdf',
			'countries' => 'FR',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '1604',
			'name' => 'PspCas13b antibody SDS ES es',
			'language' => 'es',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-ES-es-GHS_2_0.pdf',
			'countries' => 'ES',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/pspcas13b-monoclonal-antibody-50-ul'
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
	(int) 0 => array(
		'id' => '3011',
		'antibody_id' => '549',
		'name' => 'PspCas13b Antibody',
		'description' => '<p>Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13b) using a recombinant protein.</p>',
		'label1' => 'Target description',
		'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
		'label2' => 'Validation data',
		'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig1-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig2-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
		'label3' => '',
		'info3' => '',
		'format' => '50 µg',
		'catalog_number' => 'C15200250',
		'old_catalog_number' => 'C15200244',
		'sf_code' => 'C15200250-D001-000581',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '445',
		'price_USD' => '465',
		'price_GBP' => '420',
		'price_JPY' => '69710',
		'price_CNY' => '0',
		'price_AUD' => '1162',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => 'December 13, 2019',
		'slug' => 'pspcas13b-monoclonal-antibody-50-ul',
		'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'PspCas13b (CRISPR associated protein 13b from Prevotella sp.) Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
		'modified' => '2022-01-05 15:56:43',
		'created' => '2019-02-13 15:10:44'
	)
)
$pro = array(
	'id' => '3016',
	'antibody_id' => '646',
	'name' => 'PspCas13b Antibody (sample size)',
	'description' => '',
	'label1' => 'Target description',
	'info1' => '',
	'label2' => 'Validation data',
	'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig1-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig2-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
	'label3' => '',
	'info3' => '',
	'format' => '10 μg',
	'catalog_number' => 'C15200250-10',
	'old_catalog_number' => 'C15200244-10',
	'sf_code' => 'C15200250-D001-000582',
	'type' => 'FRE',
	'search_order' => '03-Antibody',
	'price_EUR' => '125',
	'price_USD' => '130',
	'price_GBP' => '120',
	'price_JPY' => '19580',
	'price_CNY' => '0',
	'price_AUD' => '325',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '',
	'slug' => 'pspcas13b-monoclonal-antibody-10-ug',
	'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
	'meta_keywords' => '',
	'meta_description' => 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available',
	'modified' => '2022-01-05 15:56:57',
	'created' => '2019-02-13 17:27:06',
	'ProductsGroup' => array(
		'id' => '293',
		'product_id' => '3016',
		'group_id' => '258'
	)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ' <span style="color:#CCC">(C15200244)</span>'
$country_code = 'US'
$other_format = array(
	'id' => '3011',
	'antibody_id' => '549',
	'name' => 'PspCas13b Antibody',
	'description' => '<p>Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13b) using a recombinant protein.</p>',
	'label1' => 'Target description',
	'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
	'label2' => 'Validation data',
	'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig1-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig2-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
	'label3' => '',
	'info3' => '',
	'format' => '50 µg',
	'catalog_number' => 'C15200250',
	'old_catalog_number' => 'C15200244',
	'sf_code' => 'C15200250-D001-000581',
	'type' => 'FRE',
	'search_order' => '03-Antibody',
	'price_EUR' => '445',
	'price_USD' => '465',
	'price_GBP' => '420',
	'price_JPY' => '69710',
	'price_CNY' => '0',
	'price_AUD' => '1162',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => 'December 13, 2019',
	'slug' => 'pspcas13b-monoclonal-antibody-50-ul',
	'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
	'meta_keywords' => '',
	'meta_description' => 'PspCas13b (CRISPR associated protein 13b from Prevotella sp.) Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
	'modified' => '2022-01-05 15:56:43',
	'created' => '2019-02-13 15:10:44'
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'ProductsApplication' => array(
		'id' => '5012',
		'product_id' => '3016',
		'application_id' => '19'
	)
)
$slugs = array(
	(int) 0 => 'western-blot-antibodies'
)
$applications = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'locale' => 'zho'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
	'id' => '1141',
	'name' => 'PspCas13b monoclonal antibody (sample size)',
	'description' => '',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/antibodies/Datasheet-pspcas13b-10ul-lot001.pdf',
	'slug' => 'pspcas13b-monoclonal-antibody-sample-datasheet',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2021-07-29 14:21:12',
	'created' => '2021-07-29 14:20:37',
	'ProductsDocument' => array(
		'id' => '3136',
		'product_id' => '3016',
		'document_id' => '1141'
	)
)
$sds = array(
	'id' => '1604',
	'name' => 'PspCas13b antibody SDS ES es',
	'language' => 'es',
	'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-ES-es-GHS_2_0.pdf',
	'countries' => 'ES',
	'modified' => '2021-08-31 09:32:37',
	'created' => '2021-08-31 09:32:37',
	'ProductsSafetySheet' => array(
		'id' => '2799',
		'product_id' => '3016',
		'safety_sheet_id' => '1604'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]
Code Context
<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'meta_keywords' => '',
	'meta_description' => 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available',
	'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
	'product' => array(
		'Product' => array(
			'id' => '3016',
			'antibody_id' => '646',
			'name' => 'PspCas13b monoclonal antibody (sample size)',
			'description' => '',
			'label1' => 'Validation data',
			'info1' => '',
			'label2' => '',
			'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig1-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig2-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
			'label3' => '',
			'info3' => '',
			'format' => '10 μg',
			'catalog_number' => 'C15200250-10',
			'old_catalog_number' => 'C15200244-10',
			'sf_code' => 'C15200250-D001-000582',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '125',
			'price_USD' => '130',
			'price_GBP' => '120',
			'price_JPY' => '19580',
			'price_CNY' => '0',
			'price_AUD' => '325',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => false,
			'last_datasheet_update' => '',
			'slug' => 'pspcas13b-monoclonal-antibody-10-ug',
			'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available',
			'modified' => '2022-01-05 15:56:57',
			'created' => '2019-02-13 17:27:06',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '646',
			'name' => 'PspCas13b monoclonal antibody (sample size)',
			'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.',
			'clonality' => '',
			'isotype' => 'IgG2a',
			'lot' => '001',
			'concentration' => '1 µg/µl',
			'reactivity' => 'Prevotella sp.',
			'type' => 'Monoclonal',
			'purity' => 'Protein G purified monoclonal antibody.',
			'classification' => '',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western blotting</td>
<td>1:1,000 &ndash; 1:4,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
			'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
			'storage_buffer' => 'TBS containing 0.02 % Na-azide.',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => 'pspcas13b-antibody-10-ug',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2021-07-29 14:11:09',
			'created' => '2021-07-29 14:11:09',
			'select_label' => '646 - PspCas13b monoclonal antibody (sample size) (001 - 1 µg/µl - Prevotella sp. - Protein G purified monoclonal antibody. - Mouse)'
		),
		'Slave' => array(),
		'Group' => array(
			'Group' => array(
				[maximum depth reached]
			),
			'Master' => array(
				[maximum depth reached]
			),
			'Product' => array(
				[maximum depth reached]
			)
		),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	),
	'meta_canonical' => 'https://www.diagenode.com/cn/p/pspcas13b-monoclonal-antibody-50-ul'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$meta_keywords = ''
$meta_description = 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available'
$meta_title = 'PspCas13b Monoclonal Antibody | Diagenode'
$product = array(
	'Product' => array(
		'id' => '3016',
		'antibody_id' => '646',
		'name' => 'PspCas13b monoclonal antibody (sample size)',
		'description' => '<p>Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13b) using a recombinant protein.</p>',
		'label1' => 'Validation data',
		'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
		'label2' => 'Validation data',
		'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig1-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig2-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
		'label3' => '',
		'info3' => '',
		'format' => '10 μg',
		'catalog_number' => 'C15200250-10',
		'old_catalog_number' => 'C15200244-10',
		'sf_code' => 'C15200250-D001-000582',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '125',
		'price_USD' => '130',
		'price_GBP' => '120',
		'price_JPY' => '19580',
		'price_CNY' => '0',
		'price_AUD' => '325',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'pspcas13b-monoclonal-antibody-10-ug',
		'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available',
		'modified' => '2022-01-05 15:56:57',
		'created' => '2019-02-13 17:27:06',
		'locale' => 'zho'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => '646',
		'name' => 'PspCas13b monoclonal antibody (sample size)',
		'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.',
		'clonality' => '',
		'isotype' => 'IgG2a',
		'lot' => '001',
		'concentration' => '1 µg/µl',
		'reactivity' => 'Prevotella sp.',
		'type' => 'Monoclonal',
		'purity' => 'Protein G purified monoclonal antibody.',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western blotting</td>
<td>1:1,000 &ndash; 1:4,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
		'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
		'storage_buffer' => 'TBS containing 0.02 % Na-azide.',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => 'pspcas13b-antibody-10-ug',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2021-07-29 14:11:09',
		'created' => '2021-07-29 14:11:09',
		'select_label' => '646 - PspCas13b monoclonal antibody (sample size) (001 - 1 µg/µl - Prevotella sp. - Protein G purified monoclonal antibody. - Mouse)'
	),
	'Slave' => array(),
	'Group' => array(
		'Group' => array(
			'id' => '258',
			'name' => 'C15200244',
			'product_id' => '3011',
			'modified' => '2019-03-19 13:37:19',
			'created' => '2019-03-19 13:37:19'
		),
		'Master' => array(
			'id' => '3011',
			'antibody_id' => '549',
			'name' => 'PspCas13b Antibody',
			'description' => '<p>Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13b) using a recombinant protein.</p>',
			'label1' => 'Target description',
			'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
			'label2' => 'Validation data',
			'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig1-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig2-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
			'label3' => '',
			'info3' => '',
			'format' => '50 µg',
			'catalog_number' => 'C15200250',
			'old_catalog_number' => 'C15200244',
			'sf_code' => 'C15200250-D001-000581',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '445',
			'price_USD' => '465',
			'price_GBP' => '420',
			'price_JPY' => '69710',
			'price_CNY' => '0',
			'price_AUD' => '1162',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => 'December 13, 2019',
			'slug' => 'pspcas13b-monoclonal-antibody-50-ul',
			'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'PspCas13b (CRISPR associated protein 13b from Prevotella sp.) Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
			'modified' => '2022-01-05 15:56:43',
			'created' => '2019-02-13 15:10:44'
		),
		'Product' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		)
	),
	'Related' => array(),
	'Application' => array(
		(int) 0 => array(
			'id' => '19',
			'position' => '10',
			'parent_id' => '40',
			'name' => 'WB',
			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
			'in_footer' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'slug' => 'western-blot-antibodies',
			'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
			'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
			'modified' => '2016-04-26 12:44:51',
			'created' => '2015-01-07 09:20:00',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '103',
			'position' => '0',
			'parent_id' => '4',
			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'all-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
			'modified' => '2019-07-03 10:55:44',
			'created' => '2015-11-02 14:49:22',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		),
		(int) 1 => array(
			'id' => '100',
			'position' => '41',
			'parent_id' => '4',
			'name' => 'CRISPR/Cas9 antibodies',
			'description' => '<p>The <strong>CRISPR/Cas genome editing system</strong> uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, this technology, can also be used to mediate up- or downregulation of specific endogenous genes or to alter histone modifications or DNA methylation. The best characterized CRISPR-associated nucleases are the <strong>Cas9 proteins</strong> from <em>Streptococcus pyogenes</em> and <em>Staphylococcus aureus</em>. Recently two new CRISPR-associated nucleases, named <strong>Cpf1</strong> (from <em>Lachnospiraceae bacterium</em> and <em>Acidaminococcus sp.</em>), have been described.<br /><br /><span>Diagenode, a dedicated supplier of high quality Cas9 antibodies, was the first company that offered the antibody Cas9 (clone 7A9). </span><span>Diagenode now offers the largest collection of optimized anti-CRISPR/Cas antibodies, validated in a number of different applications including Western blot (WB), immunofluorescence (IF), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP).</span></p>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3><a name="4reasons"></a>Four critical reasons to use anti-CRISPR/Cas9 antibodies</h3>
<p>CRISPR/Cas9 genome editing allows for double-stranded DNA breaks at specific sequences to efficiently disrupt, excise, mutate, insert, or replace genes. The precision of transfection and the level of Cas9 expression should be controlled during the editing processes using specific anti-CRISPR/Cas9 antibodies.</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-1.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #1</strong> Check the transfection success of the Cas9 protein</h4>
<p>Check <strong>Cas9 expression level</strong> using <strong>Western blot</strong> with validated anti-Cas9 antibodies.</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-2.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #2</strong> Check that the Cas9 protein was delivered to the nucleus</h4>
<p>Determine the nuclear localization of Cas9 protein via <strong>IF</strong> or <strong>IHC</strong> using validated anti-Cas9 antibodies</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-3.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #3</strong> Check the Cas9 expression level</h4>
<p><strong>Transient systems</strong>: Check that <strong>Cas9 expression</strong> was transient by using <strong>Western blot</strong> and anti-Cas9 antibodies. Prolonged Cas9 expression will lead to more off-target mutations.</p>
<p><strong>Stable transformants</strong>: Isolate several clones and screen to check the level of <strong>Cas9 expression</strong> using <strong>Western blot</strong> and specific anti-Cas9 antibodies. High level of Cas9 expression can lead to non-specific activity.</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-4.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #4</strong> Check the binding specificity of Cas9</h4>
<p>Test the binding specificity with an sgRNA of choice by using the <strong>ChIP-grade anti-Cas9 antibody</strong> and the primers for targeted and non-targeted region to see if Cas9 is bound to the correct region.</p>
<p>For more details go to the page <strong><a href="https://www.diagenode.com/en/categories/selection-of-guide-rna-by-ChIP">Selection of guide RNA by ChIP</a></strong>.</p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>Published examples of the use of anti-Cas9 antibodies:</h3>
<ul>
<li><a href="../publications/view/2823">A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing</a></li>
<li><a href="../publications/view/2792">A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage</a></li>
<li><a href="../publications/view/2765">Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection</a></li>
<li><a href="../publications/view/2679">Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells</a></li>
<li><a href="../publications/view/2645">Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis</a></li>
<li><a href="../publications/view/2961">An inducible lentiviral guide RNA platform enables the identification of tumor-essential genes and tumor-promoting mutations in vivo</a></li>
</ul>
<h5><strong>Diagenode offers the largest collection of validated anti-CRISPR/Cas antibodies, validated in different applications (WB, IF, IP and ChIP).</strong></h5>
</div>
</div>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'crispr-cas9',
			'cookies_tag_id' => '1',
			'meta_keywords' => 'crispr cas9 antibodies,cas9 applications,cas9 protocol,cas9 review',
			'meta_description' => 'Diagenode offers a broad range of highly validated CRISPR/Cas9 Antibodies, Recommended for different applications (WB, IF, IP and chromatin IP). These Antibodies have been raised against the N- or C-terminus of the Cas9 Nuclease.',
			'meta_title' => 'Best CRISPR-Cas9 Antibodies on the Market | Diagenode',
			'modified' => '2020-08-03 15:29:26',
			'created' => '2015-10-19 14:55:29',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '134',
			'position' => '0',
			'parent_id' => '100',
			'name' => 'RNA-targeting CRISPR antibodies',
			'description' => '<p>The latest tool for CRISPR gene editing is a new CRISPR-C2C2 (Cas13a) system which utilizes RNA-guided RNA-targeting for RNA knockdown and binding in cells. C2C2 (Cas13a) may be useful for isolating specific RNA sequences or even studying single RNA molecules with high specificity. Unlike editing DNA as with Cas9 which makes permanent genomic changes,&nbsp; targeting RNA with C2C2 allows temporarily altering the amount of protein potentially offering a better therapeutic approach in the future.&nbsp;</p>
<p>Use Diagenode's new C2C2 (Cas13a) polyclonal antibody to determine precision and success metrics in CRISPR experiments.</p>',
			'no_promo' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'antibodies-against-rna-targeting-crispr',
			'cookies_tag_id' => '1',
			'meta_keywords' => '',
			'meta_description' => '',
			'meta_title' => 'Antibodies against RNA-targeting CRISPR',
			'modified' => '2018-04-26 15:44:54',
			'created' => '2018-03-05 11:46:15',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '102',
			'position' => '1',
			'parent_id' => '4',
			'name' => 'Sample size antibodies',
			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => true,
			'is_antibody' => true,
			'slug' => 'sample-size-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
			'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
			'meta_title' => 'Sample-size Antibodies | Diagenode',
			'modified' => '2019-07-03 10:57:05',
			'created' => '2015-10-27 12:13:34',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '1141',
			'name' => 'PspCas13b monoclonal antibody (sample size)',
			'description' => '',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/antibodies/Datasheet-pspcas13b-10ul-lot001.pdf',
			'slug' => 'pspcas13b-monoclonal-antibody-sample-datasheet',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2021-07-29 14:21:12',
			'created' => '2021-07-29 14:20:37',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '250',
			'name' => 'product/antibodies/antibody.png',
			'alt' => 'Mouse IgG',
			'modified' => '2020-11-27 07:00:09',
			'created' => '2015-07-17 10:12:18',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '1606',
			'name' => 'PspCas13b antibody SDS GB en',
			'language' => 'en',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-GB-en-GHS_2_0.pdf',
			'countries' => 'GB',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1608',
			'name' => 'PspCas13b antibody SDS US en',
			'language' => 'en',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-US-en-GHS_2_0.pdf',
			'countries' => 'US',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1603',
			'name' => 'PspCas13b antibody SDS DE de',
			'language' => 'de',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-DE-de-GHS_2_0.pdf',
			'countries' => 'DE',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1607',
			'name' => 'PspCas13b antibody SDS JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-JP-ja-GHS_3_0.pdf',
			'countries' => 'JP',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '1602',
			'name' => 'PspCas13b antibody SDS BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-BE-nl-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '1601',
			'name' => 'PspCas13b antibody SDS BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-BE-fr-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '1605',
			'name' => 'PspCas13b antibody SDS FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-FR-fr-GHS_2_0.pdf',
			'countries' => 'FR',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '1604',
			'name' => 'PspCas13b antibody SDS ES es',
			'language' => 'es',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-ES-es-GHS_2_0.pdf',
			'countries' => 'ES',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/pspcas13b-monoclonal-antibody-50-ul'
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
	(int) 0 => array(
		'id' => '3011',
		'antibody_id' => '549',
		'name' => 'PspCas13b Antibody',
		'description' => '<p>Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13b) using a recombinant protein.</p>',
		'label1' => 'Target description',
		'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
		'label2' => 'Validation data',
		'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig1-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig2-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
		'label3' => '',
		'info3' => '',
		'format' => '50 µg',
		'catalog_number' => 'C15200250',
		'old_catalog_number' => 'C15200244',
		'sf_code' => 'C15200250-D001-000581',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '445',
		'price_USD' => '465',
		'price_GBP' => '420',
		'price_JPY' => '69710',
		'price_CNY' => '0',
		'price_AUD' => '1162',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => 'December 13, 2019',
		'slug' => 'pspcas13b-monoclonal-antibody-50-ul',
		'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'PspCas13b (CRISPR associated protein 13b from Prevotella sp.) Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
		'modified' => '2022-01-05 15:56:43',
		'created' => '2019-02-13 15:10:44'
	)
)
$pro = array(
	'id' => '3016',
	'antibody_id' => '646',
	'name' => 'PspCas13b Antibody (sample size)',
	'description' => '',
	'label1' => 'Target description',
	'info1' => '',
	'label2' => 'Validation data',
	'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig1-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig2-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
	'label3' => '',
	'info3' => '',
	'format' => '10 μg',
	'catalog_number' => 'C15200250-10',
	'old_catalog_number' => 'C15200244-10',
	'sf_code' => 'C15200250-D001-000582',
	'type' => 'FRE',
	'search_order' => '03-Antibody',
	'price_EUR' => '125',
	'price_USD' => '130',
	'price_GBP' => '120',
	'price_JPY' => '19580',
	'price_CNY' => '0',
	'price_AUD' => '325',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '',
	'slug' => 'pspcas13b-monoclonal-antibody-10-ug',
	'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
	'meta_keywords' => '',
	'meta_description' => 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available',
	'modified' => '2022-01-05 15:56:57',
	'created' => '2019-02-13 17:27:06',
	'ProductsGroup' => array(
		'id' => '293',
		'product_id' => '3016',
		'group_id' => '258'
	)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ' <span style="color:#CCC">(C15200244)</span>'
$country_code = 'US'
$other_format = array(
	'id' => '3011',
	'antibody_id' => '549',
	'name' => 'PspCas13b Antibody',
	'description' => '<p>Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13b) using a recombinant protein.</p>',
	'label1' => 'Target description',
	'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
	'label2' => 'Validation data',
	'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig1-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig2-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
	'label3' => '',
	'info3' => '',
	'format' => '50 µg',
	'catalog_number' => 'C15200250',
	'old_catalog_number' => 'C15200244',
	'sf_code' => 'C15200250-D001-000581',
	'type' => 'FRE',
	'search_order' => '03-Antibody',
	'price_EUR' => '445',
	'price_USD' => '465',
	'price_GBP' => '420',
	'price_JPY' => '69710',
	'price_CNY' => '0',
	'price_AUD' => '1162',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => 'December 13, 2019',
	'slug' => 'pspcas13b-monoclonal-antibody-50-ul',
	'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
	'meta_keywords' => '',
	'meta_description' => 'PspCas13b (CRISPR associated protein 13b from Prevotella sp.) Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
	'modified' => '2022-01-05 15:56:43',
	'created' => '2019-02-13 15:10:44'
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'ProductsApplication' => array(
		'id' => '5012',
		'product_id' => '3016',
		'application_id' => '19'
	)
)
$slugs = array(
	(int) 0 => 'western-blot-antibodies'
)
$applications = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'locale' => 'zho'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
	'id' => '1141',
	'name' => 'PspCas13b monoclonal antibody (sample size)',
	'description' => '',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/antibodies/Datasheet-pspcas13b-10ul-lot001.pdf',
	'slug' => 'pspcas13b-monoclonal-antibody-sample-datasheet',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2021-07-29 14:21:12',
	'created' => '2021-07-29 14:20:37',
	'ProductsDocument' => array(
		'id' => '3136',
		'product_id' => '3016',
		'document_id' => '1141'
	)
)
$sds = array(
	'id' => '1604',
	'name' => 'PspCas13b antibody SDS ES es',
	'language' => 'es',
	'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-ES-es-GHS_2_0.pdf',
	'countries' => 'ES',
	'modified' => '2021-08-31 09:32:37',
	'created' => '2021-08-31 09:32:37',
	'ProductsSafetySheet' => array(
		'id' => '2799',
		'product_id' => '3016',
		'safety_sheet_id' => '1604'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]
Code Context
<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'meta_keywords' => '',
	'meta_description' => 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available',
	'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
	'product' => array(
		'Product' => array(
			'id' => '3016',
			'antibody_id' => '646',
			'name' => 'PspCas13b monoclonal antibody (sample size)',
			'description' => '',
			'label1' => 'Validation data',
			'info1' => '',
			'label2' => '',
			'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig1-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig2-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
			'label3' => '',
			'info3' => '',
			'format' => '10 μg',
			'catalog_number' => 'C15200250-10',
			'old_catalog_number' => 'C15200244-10',
			'sf_code' => 'C15200250-D001-000582',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '125',
			'price_USD' => '130',
			'price_GBP' => '120',
			'price_JPY' => '19580',
			'price_CNY' => '0',
			'price_AUD' => '325',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => false,
			'last_datasheet_update' => '',
			'slug' => 'pspcas13b-monoclonal-antibody-10-ug',
			'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available',
			'modified' => '2022-01-05 15:56:57',
			'created' => '2019-02-13 17:27:06',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '646',
			'name' => 'PspCas13b monoclonal antibody (sample size)',
			'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.',
			'clonality' => '',
			'isotype' => 'IgG2a',
			'lot' => '001',
			'concentration' => '1 µg/µl',
			'reactivity' => 'Prevotella sp.',
			'type' => 'Monoclonal',
			'purity' => 'Protein G purified monoclonal antibody.',
			'classification' => '',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western blotting</td>
<td>1:1,000 &ndash; 1:4,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
			'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
			'storage_buffer' => 'TBS containing 0.02 % Na-azide.',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => 'pspcas13b-antibody-10-ug',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2021-07-29 14:11:09',
			'created' => '2021-07-29 14:11:09',
			'select_label' => '646 - PspCas13b monoclonal antibody (sample size) (001 - 1 µg/µl - Prevotella sp. - Protein G purified monoclonal antibody. - Mouse)'
		),
		'Slave' => array(),
		'Group' => array(
			'Group' => array(
				[maximum depth reached]
			),
			'Master' => array(
				[maximum depth reached]
			),
			'Product' => array(
				[maximum depth reached]
			)
		),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	),
	'meta_canonical' => 'https://www.diagenode.com/cn/p/pspcas13b-monoclonal-antibody-50-ul'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$meta_keywords = ''
$meta_description = 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available'
$meta_title = 'PspCas13b Monoclonal Antibody | Diagenode'
$product = array(
	'Product' => array(
		'id' => '3016',
		'antibody_id' => '646',
		'name' => 'PspCas13b monoclonal antibody (sample size)',
		'description' => '<p>Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13b) using a recombinant protein.</p>',
		'label1' => 'Validation data',
		'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
		'label2' => 'Validation data',
		'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig1-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig2-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
		'label3' => '',
		'info3' => '',
		'format' => '10 μg',
		'catalog_number' => 'C15200250-10',
		'old_catalog_number' => 'C15200244-10',
		'sf_code' => 'C15200250-D001-000582',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '125',
		'price_USD' => '130',
		'price_GBP' => '120',
		'price_JPY' => '19580',
		'price_CNY' => '0',
		'price_AUD' => '325',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'pspcas13b-monoclonal-antibody-10-ug',
		'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available',
		'modified' => '2022-01-05 15:56:57',
		'created' => '2019-02-13 17:27:06',
		'locale' => 'zho'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => '646',
		'name' => 'PspCas13b monoclonal antibody (sample size)',
		'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.',
		'clonality' => '',
		'isotype' => 'IgG2a',
		'lot' => '001',
		'concentration' => '1 µg/µl',
		'reactivity' => 'Prevotella sp.',
		'type' => 'Monoclonal',
		'purity' => 'Protein G purified monoclonal antibody.',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western blotting</td>
<td>1:1,000 &ndash; 1:4,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
		'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
		'storage_buffer' => 'TBS containing 0.02 % Na-azide.',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => 'pspcas13b-antibody-10-ug',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2021-07-29 14:11:09',
		'created' => '2021-07-29 14:11:09',
		'select_label' => '646 - PspCas13b monoclonal antibody (sample size) (001 - 1 µg/µl - Prevotella sp. - Protein G purified monoclonal antibody. - Mouse)'
	),
	'Slave' => array(),
	'Group' => array(
		'Group' => array(
			'id' => '258',
			'name' => 'C15200244',
			'product_id' => '3011',
			'modified' => '2019-03-19 13:37:19',
			'created' => '2019-03-19 13:37:19'
		),
		'Master' => array(
			'id' => '3011',
			'antibody_id' => '549',
			'name' => 'PspCas13b Antibody',
			'description' => '<p>Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13b) using a recombinant protein.</p>',
			'label1' => 'Target description',
			'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
			'label2' => 'Validation data',
			'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig1-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig2-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
			'label3' => '',
			'info3' => '',
			'format' => '50 µg',
			'catalog_number' => 'C15200250',
			'old_catalog_number' => 'C15200244',
			'sf_code' => 'C15200250-D001-000581',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '445',
			'price_USD' => '465',
			'price_GBP' => '420',
			'price_JPY' => '69710',
			'price_CNY' => '0',
			'price_AUD' => '1162',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => 'December 13, 2019',
			'slug' => 'pspcas13b-monoclonal-antibody-50-ul',
			'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'PspCas13b (CRISPR associated protein 13b from Prevotella sp.) Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
			'modified' => '2022-01-05 15:56:43',
			'created' => '2019-02-13 15:10:44'
		),
		'Product' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		)
	),
	'Related' => array(),
	'Application' => array(
		(int) 0 => array(
			'id' => '19',
			'position' => '10',
			'parent_id' => '40',
			'name' => 'WB',
			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
			'in_footer' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'slug' => 'western-blot-antibodies',
			'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
			'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
			'modified' => '2016-04-26 12:44:51',
			'created' => '2015-01-07 09:20:00',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '103',
			'position' => '0',
			'parent_id' => '4',
			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'all-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
			'modified' => '2019-07-03 10:55:44',
			'created' => '2015-11-02 14:49:22',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		),
		(int) 1 => array(
			'id' => '100',
			'position' => '41',
			'parent_id' => '4',
			'name' => 'CRISPR/Cas9 antibodies',
			'description' => '<p>The <strong>CRISPR/Cas genome editing system</strong> uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, this technology, can also be used to mediate up- or downregulation of specific endogenous genes or to alter histone modifications or DNA methylation. The best characterized CRISPR-associated nucleases are the <strong>Cas9 proteins</strong> from <em>Streptococcus pyogenes</em> and <em>Staphylococcus aureus</em>. Recently two new CRISPR-associated nucleases, named <strong>Cpf1</strong> (from <em>Lachnospiraceae bacterium</em> and <em>Acidaminococcus sp.</em>), have been described.<br /><br /><span>Diagenode, a dedicated supplier of high quality Cas9 antibodies, was the first company that offered the antibody Cas9 (clone 7A9). </span><span>Diagenode now offers the largest collection of optimized anti-CRISPR/Cas antibodies, validated in a number of different applications including Western blot (WB), immunofluorescence (IF), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP).</span></p>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3><a name="4reasons"></a>Four critical reasons to use anti-CRISPR/Cas9 antibodies</h3>
<p>CRISPR/Cas9 genome editing allows for double-stranded DNA breaks at specific sequences to efficiently disrupt, excise, mutate, insert, or replace genes. The precision of transfection and the level of Cas9 expression should be controlled during the editing processes using specific anti-CRISPR/Cas9 antibodies.</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-1.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #1</strong> Check the transfection success of the Cas9 protein</h4>
<p>Check <strong>Cas9 expression level</strong> using <strong>Western blot</strong> with validated anti-Cas9 antibodies.</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-2.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #2</strong> Check that the Cas9 protein was delivered to the nucleus</h4>
<p>Determine the nuclear localization of Cas9 protein via <strong>IF</strong> or <strong>IHC</strong> using validated anti-Cas9 antibodies</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-3.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #3</strong> Check the Cas9 expression level</h4>
<p><strong>Transient systems</strong>: Check that <strong>Cas9 expression</strong> was transient by using <strong>Western blot</strong> and anti-Cas9 antibodies. Prolonged Cas9 expression will lead to more off-target mutations.</p>
<p><strong>Stable transformants</strong>: Isolate several clones and screen to check the level of <strong>Cas9 expression</strong> using <strong>Western blot</strong> and specific anti-Cas9 antibodies. High level of Cas9 expression can lead to non-specific activity.</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-4.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #4</strong> Check the binding specificity of Cas9</h4>
<p>Test the binding specificity with an sgRNA of choice by using the <strong>ChIP-grade anti-Cas9 antibody</strong> and the primers for targeted and non-targeted region to see if Cas9 is bound to the correct region.</p>
<p>For more details go to the page <strong><a href="https://www.diagenode.com/en/categories/selection-of-guide-rna-by-ChIP">Selection of guide RNA by ChIP</a></strong>.</p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>Published examples of the use of anti-Cas9 antibodies:</h3>
<ul>
<li><a href="../publications/view/2823">A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing</a></li>
<li><a href="../publications/view/2792">A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage</a></li>
<li><a href="../publications/view/2765">Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection</a></li>
<li><a href="../publications/view/2679">Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells</a></li>
<li><a href="../publications/view/2645">Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis</a></li>
<li><a href="../publications/view/2961">An inducible lentiviral guide RNA platform enables the identification of tumor-essential genes and tumor-promoting mutations in vivo</a></li>
</ul>
<h5><strong>Diagenode offers the largest collection of validated anti-CRISPR/Cas antibodies, validated in different applications (WB, IF, IP and ChIP).</strong></h5>
</div>
</div>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'crispr-cas9',
			'cookies_tag_id' => '1',
			'meta_keywords' => 'crispr cas9 antibodies,cas9 applications,cas9 protocol,cas9 review',
			'meta_description' => 'Diagenode offers a broad range of highly validated CRISPR/Cas9 Antibodies, Recommended for different applications (WB, IF, IP and chromatin IP). These Antibodies have been raised against the N- or C-terminus of the Cas9 Nuclease.',
			'meta_title' => 'Best CRISPR-Cas9 Antibodies on the Market | Diagenode',
			'modified' => '2020-08-03 15:29:26',
			'created' => '2015-10-19 14:55:29',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '134',
			'position' => '0',
			'parent_id' => '100',
			'name' => 'RNA-targeting CRISPR antibodies',
			'description' => '<p>The latest tool for CRISPR gene editing is a new CRISPR-C2C2 (Cas13a) system which utilizes RNA-guided RNA-targeting for RNA knockdown and binding in cells. C2C2 (Cas13a) may be useful for isolating specific RNA sequences or even studying single RNA molecules with high specificity. Unlike editing DNA as with Cas9 which makes permanent genomic changes,&nbsp; targeting RNA with C2C2 allows temporarily altering the amount of protein potentially offering a better therapeutic approach in the future.&nbsp;</p>
<p>Use Diagenode's new C2C2 (Cas13a) polyclonal antibody to determine precision and success metrics in CRISPR experiments.</p>',
			'no_promo' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'antibodies-against-rna-targeting-crispr',
			'cookies_tag_id' => '1',
			'meta_keywords' => '',
			'meta_description' => '',
			'meta_title' => 'Antibodies against RNA-targeting CRISPR',
			'modified' => '2018-04-26 15:44:54',
			'created' => '2018-03-05 11:46:15',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '102',
			'position' => '1',
			'parent_id' => '4',
			'name' => 'Sample size antibodies',
			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => true,
			'is_antibody' => true,
			'slug' => 'sample-size-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
			'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
			'meta_title' => 'Sample-size Antibodies | Diagenode',
			'modified' => '2019-07-03 10:57:05',
			'created' => '2015-10-27 12:13:34',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '1141',
			'name' => 'PspCas13b monoclonal antibody (sample size)',
			'description' => '',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/antibodies/Datasheet-pspcas13b-10ul-lot001.pdf',
			'slug' => 'pspcas13b-monoclonal-antibody-sample-datasheet',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2021-07-29 14:21:12',
			'created' => '2021-07-29 14:20:37',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '250',
			'name' => 'product/antibodies/antibody.png',
			'alt' => 'Mouse IgG',
			'modified' => '2020-11-27 07:00:09',
			'created' => '2015-07-17 10:12:18',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '1606',
			'name' => 'PspCas13b antibody SDS GB en',
			'language' => 'en',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-GB-en-GHS_2_0.pdf',
			'countries' => 'GB',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1608',
			'name' => 'PspCas13b antibody SDS US en',
			'language' => 'en',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-US-en-GHS_2_0.pdf',
			'countries' => 'US',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1603',
			'name' => 'PspCas13b antibody SDS DE de',
			'language' => 'de',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-DE-de-GHS_2_0.pdf',
			'countries' => 'DE',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1607',
			'name' => 'PspCas13b antibody SDS JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-JP-ja-GHS_3_0.pdf',
			'countries' => 'JP',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '1602',
			'name' => 'PspCas13b antibody SDS BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-BE-nl-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '1601',
			'name' => 'PspCas13b antibody SDS BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-BE-fr-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '1605',
			'name' => 'PspCas13b antibody SDS FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-FR-fr-GHS_2_0.pdf',
			'countries' => 'FR',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '1604',
			'name' => 'PspCas13b antibody SDS ES es',
			'language' => 'es',
			'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-ES-es-GHS_2_0.pdf',
			'countries' => 'ES',
			'modified' => '2021-08-31 09:32:37',
			'created' => '2021-08-31 09:32:37',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/pspcas13b-monoclonal-antibody-50-ul'
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
	(int) 0 => array(
		'id' => '3011',
		'antibody_id' => '549',
		'name' => 'PspCas13b Antibody',
		'description' => '<p>Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13b) using a recombinant protein.</p>',
		'label1' => 'Target description',
		'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
		'label2' => 'Validation data',
		'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig1-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig2-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
		'label3' => '',
		'info3' => '',
		'format' => '50 µg',
		'catalog_number' => 'C15200250',
		'old_catalog_number' => 'C15200244',
		'sf_code' => 'C15200250-D001-000581',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '445',
		'price_USD' => '465',
		'price_GBP' => '420',
		'price_JPY' => '69710',
		'price_CNY' => '0',
		'price_AUD' => '1162',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => 'December 13, 2019',
		'slug' => 'pspcas13b-monoclonal-antibody-50-ul',
		'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'PspCas13b (CRISPR associated protein 13b from Prevotella sp.) Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
		'modified' => '2022-01-05 15:56:43',
		'created' => '2019-02-13 15:10:44'
	)
)
$pro = array(
	'id' => '3016',
	'antibody_id' => '646',
	'name' => 'PspCas13b Antibody (sample size)',
	'description' => '',
	'label1' => 'Target description',
	'info1' => '',
	'label2' => 'Validation data',
	'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig1-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig2-lot001.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
	'label3' => '',
	'info3' => '',
	'format' => '10 μg',
	'catalog_number' => 'C15200250-10',
	'old_catalog_number' => 'C15200244-10',
	'sf_code' => 'C15200250-D001-000582',
	'type' => 'FRE',
	'search_order' => '03-Antibody',
	'price_EUR' => '125',
	'price_USD' => '130',
	'price_GBP' => '120',
	'price_JPY' => '19580',
	'price_CNY' => '0',
	'price_AUD' => '325',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '',
	'slug' => 'pspcas13b-monoclonal-antibody-10-ug',
	'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
	'meta_keywords' => '',
	'meta_description' => 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available',
	'modified' => '2022-01-05 15:56:57',
	'created' => '2019-02-13 17:27:06',
	'ProductsGroup' => array(
		'id' => '293',
		'product_id' => '3016',
		'group_id' => '258'
	)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ' <span style="color:#CCC">(C15200244)</span>'
$country_code = 'US'
$other_format = array(
	'id' => '3011',
	'antibody_id' => '549',
	'name' => 'PspCas13b Antibody',
	'description' => '<p>Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13b) using a recombinant protein.</p>',
	'label1' => 'Target description',
	'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
	'label2' => 'Validation data',
	'info2' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig1-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig2-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
</div>
</div>',
	'label3' => '',
	'info3' => '',
	'format' => '50 µg',
	'catalog_number' => 'C15200250',
	'old_catalog_number' => 'C15200244',
	'sf_code' => 'C15200250-D001-000581',
	'type' => 'FRE',
	'search_order' => '03-Antibody',
	'price_EUR' => '445',
	'price_USD' => '465',
	'price_GBP' => '420',
	'price_JPY' => '69710',
	'price_CNY' => '0',
	'price_AUD' => '1162',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => 'December 13, 2019',
	'slug' => 'pspcas13b-monoclonal-antibody-50-ul',
	'meta_title' => 'PspCas13b Monoclonal Antibody | Diagenode',
	'meta_keywords' => '',
	'meta_description' => 'PspCas13b (CRISPR associated protein 13b from Prevotella sp.) Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
	'modified' => '2022-01-05 15:56:43',
	'created' => '2019-02-13 15:10:44'
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'ProductsApplication' => array(
		'id' => '5012',
		'product_id' => '3016',
		'application_id' => '19'
	)
)
$slugs = array(
	(int) 0 => 'western-blot-antibodies'
)
$applications = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'locale' => 'zho'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
	'id' => '1141',
	'name' => 'PspCas13b monoclonal antibody (sample size)',
	'description' => '',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/antibodies/Datasheet-pspcas13b-10ul-lot001.pdf',
	'slug' => 'pspcas13b-monoclonal-antibody-sample-datasheet',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2021-07-29 14:21:12',
	'created' => '2021-07-29 14:20:37',
	'ProductsDocument' => array(
		'id' => '3136',
		'product_id' => '3016',
		'document_id' => '1141'
	)
)
$sds = array(
	'id' => '1604',
	'name' => 'PspCas13b antibody SDS ES es',
	'language' => 'es',
	'url' => 'files/SDS/PspCas13b/SDS-C15200250-PspCas13b_Antibody-ES-es-GHS_2_0.pdf',
	'countries' => 'ES',
	'modified' => '2021-08-31 09:32:37',
	'created' => '2021-08-31 09:32:37',
	'ProductsSafetySheet' => array(
		'id' => '2799',
		'product_id' => '3016',
		'safety_sheet_id' => '1604'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118