PspCas13b monoclonal antibody (sample size)

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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<td>1:1,000 &ndash; 1:4,000</td>
<td>Fig 1, 2</td>
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		'label1' => 'Validation data',
		'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/pspcas13b-fig2-lot001.png" alt="WB result" /></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<td>1:1,000 &ndash; 1:4,000</td>
<td>Fig 1, 2</td>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig1-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig2-WB.png" alt="WB result" /></p>
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<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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			'description' => '<p>The <strong>CRISPR/Cas genome editing system</strong> uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, this technology, can also be used to mediate up- or downregulation of specific endogenous genes or to alter histone modifications or DNA methylation. The best characterized CRISPR-associated nucleases are the <strong>Cas9 proteins</strong> from <em>Streptococcus pyogenes</em> and <em>Staphylococcus aureus</em>. Recently two new CRISPR-associated nucleases, named <strong>Cpf1</strong> (from <em>Lachnospiraceae bacterium</em> and <em>Acidaminococcus sp.</em>), have been described.<br /><br /><span>Diagenode, a dedicated supplier of high quality Cas9 antibodies, was the first company that offered the antibody Cas9 (clone 7A9). </span><span>Diagenode now offers the largest collection of optimized anti-CRISPR/Cas antibodies, validated in a number of different applications including Western blot (WB), immunofluorescence (IF), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP).</span></p>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3><a name="4reasons"></a>Four critical reasons to use anti-CRISPR/Cas9 antibodies</h3>
<p>CRISPR/Cas9 genome editing allows for double-stranded DNA breaks at specific sequences to efficiently disrupt, excise, mutate, insert, or replace genes. The precision of transfection and the level of Cas9 expression should be controlled during the editing processes using specific anti-CRISPR/Cas9 antibodies.</p>
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<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-1.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #1</strong> Check the transfection success of the Cas9 protein</h4>
<p>Check <strong>Cas9 expression level</strong> using <strong>Western blot</strong> with validated anti-Cas9 antibodies.</p>
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<h4><strong>Reason #2</strong> Check that the Cas9 protein was delivered to the nucleus</h4>
<p>Determine the nuclear localization of Cas9 protein via <strong>IF</strong> or <strong>IHC</strong> using validated anti-Cas9 antibodies</p>
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<h4><strong>Reason #3</strong> Check the Cas9 expression level</h4>
<p><strong>Transient systems</strong>: Check that <strong>Cas9 expression</strong> was transient by using <strong>Western blot</strong> and anti-Cas9 antibodies. Prolonged Cas9 expression will lead to more off-target mutations.</p>
<p><strong>Stable transformants</strong>: Isolate several clones and screen to check the level of <strong>Cas9 expression</strong> using <strong>Western blot</strong> and specific anti-Cas9 antibodies. High level of Cas9 expression can lead to non-specific activity.</p>
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<h4><strong>Reason #4</strong> Check the binding specificity of Cas9</h4>
<p>Test the binding specificity with an sgRNA of choice by using the <strong>ChIP-grade anti-Cas9 antibody</strong> and the primers for targeted and non-targeted region to see if Cas9 is bound to the correct region.</p>
<p>For more details go to the page <strong><a href="https://www.diagenode.com/en/categories/selection-of-guide-rna-by-ChIP">Selection of guide RNA by ChIP</a></strong>.</p>
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<h3>Published examples of the use of anti-Cas9 antibodies:</h3>
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<li><a href="../publications/view/2823">A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing</a></li>
<li><a href="../publications/view/2792">A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage</a></li>
<li><a href="../publications/view/2765">Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection</a></li>
<li><a href="../publications/view/2679">Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells</a></li>
<li><a href="../publications/view/2645">Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis</a></li>
<li><a href="../publications/view/2961">An inducible lentiviral guide RNA platform enables the identification of tumor-essential genes and tumor-promoting mutations in vivo</a></li>
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<h5><strong>Diagenode offers the largest collection of validated anti-CRISPR/Cas antibodies, validated in different applications (WB, IF, IP and ChIP).</strong></h5>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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			'description' => '<p>The <strong>CRISPR/Cas genome editing system</strong> uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, this technology, can also be used to mediate up- or downregulation of specific endogenous genes or to alter histone modifications or DNA methylation. The best characterized CRISPR-associated nucleases are the <strong>Cas9 proteins</strong> from <em>Streptococcus pyogenes</em> and <em>Staphylococcus aureus</em>. Recently two new CRISPR-associated nucleases, named <strong>Cpf1</strong> (from <em>Lachnospiraceae bacterium</em> and <em>Acidaminococcus sp.</em>), have been described.<br /><br /><span>Diagenode, a dedicated supplier of high quality Cas9 antibodies, was the first company that offered the antibody Cas9 (clone 7A9). </span><span>Diagenode now offers the largest collection of optimized anti-CRISPR/Cas antibodies, validated in a number of different applications including Western blot (WB), immunofluorescence (IF), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP).</span></p>
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<div class="small-12 medium-12 large-12 columns">
<h3><a name="4reasons"></a>Four critical reasons to use anti-CRISPR/Cas9 antibodies</h3>
<p>CRISPR/Cas9 genome editing allows for double-stranded DNA breaks at specific sequences to efficiently disrupt, excise, mutate, insert, or replace genes. The precision of transfection and the level of Cas9 expression should be controlled during the editing processes using specific anti-CRISPR/Cas9 antibodies.</p>
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<h4><strong>Reason #1</strong> Check the transfection success of the Cas9 protein</h4>
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<h4><strong>Reason #2</strong> Check that the Cas9 protein was delivered to the nucleus</h4>
<p>Determine the nuclear localization of Cas9 protein via <strong>IF</strong> or <strong>IHC</strong> using validated anti-Cas9 antibodies</p>
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<h4><strong>Reason #3</strong> Check the Cas9 expression level</h4>
<p><strong>Transient systems</strong>: Check that <strong>Cas9 expression</strong> was transient by using <strong>Western blot</strong> and anti-Cas9 antibodies. Prolonged Cas9 expression will lead to more off-target mutations.</p>
<p><strong>Stable transformants</strong>: Isolate several clones and screen to check the level of <strong>Cas9 expression</strong> using <strong>Western blot</strong> and specific anti-Cas9 antibodies. High level of Cas9 expression can lead to non-specific activity.</p>
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<h4><strong>Reason #4</strong> Check the binding specificity of Cas9</h4>
<p>Test the binding specificity with an sgRNA of choice by using the <strong>ChIP-grade anti-Cas9 antibody</strong> and the primers for targeted and non-targeted region to see if Cas9 is bound to the correct region.</p>
<p>For more details go to the page <strong><a href="https://www.diagenode.com/en/categories/selection-of-guide-rna-by-ChIP">Selection of guide RNA by ChIP</a></strong>.</p>
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<h3>Published examples of the use of anti-Cas9 antibodies:</h3>
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<li><a href="../publications/view/2823">A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing</a></li>
<li><a href="../publications/view/2792">A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage</a></li>
<li><a href="../publications/view/2765">Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection</a></li>
<li><a href="../publications/view/2679">Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells</a></li>
<li><a href="../publications/view/2645">Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis</a></li>
<li><a href="../publications/view/2961">An inducible lentiviral guide RNA platform enables the identification of tumor-essential genes and tumor-promoting mutations in vivo</a></li>
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<h5><strong>Diagenode offers the largest collection of validated anti-CRISPR/Cas antibodies, validated in different applications (WB, IF, IP and ChIP).</strong></h5>
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		'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig1-WB.png" alt="WB result" /></p>
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<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
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ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]
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		'meta_description' => 'PspCas13b (CRISPR associated protein 13b) Monoclonal Antibody  validated  in WB.  Batch-specific data available on the website. Sample size available',
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		'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.',
		'clonality' => '',
		'isotype' => 'IgG2a',
		'lot' => '001',
		'concentration' => '1 µg/µl',
		'reactivity' => 'Prevotella sp.',
		'type' => 'Monoclonal',
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<td>Western blotting</td>
<td>1:1,000 &ndash; 1:4,000</td>
<td>Fig 1, 2</td>
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		'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
		'storage_buffer' => 'TBS containing 0.02 % Na-azide.',
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			'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig1-WB.png" alt="WB result" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig2-WB.png" alt="WB result" /></p>
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<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Batch-specific data is available on the website</li>
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			'description' => '<p>The <strong>CRISPR/Cas genome editing system</strong> uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, this technology, can also be used to mediate up- or downregulation of specific endogenous genes or to alter histone modifications or DNA methylation. The best characterized CRISPR-associated nucleases are the <strong>Cas9 proteins</strong> from <em>Streptococcus pyogenes</em> and <em>Staphylococcus aureus</em>. Recently two new CRISPR-associated nucleases, named <strong>Cpf1</strong> (from <em>Lachnospiraceae bacterium</em> and <em>Acidaminococcus sp.</em>), have been described.<br /><br /><span>Diagenode, a dedicated supplier of high quality Cas9 antibodies, was the first company that offered the antibody Cas9 (clone 7A9). </span><span>Diagenode now offers the largest collection of optimized anti-CRISPR/Cas antibodies, validated in a number of different applications including Western blot (WB), immunofluorescence (IF), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP).</span></p>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3><a name="4reasons"></a>Four critical reasons to use anti-CRISPR/Cas9 antibodies</h3>
<p>CRISPR/Cas9 genome editing allows for double-stranded DNA breaks at specific sequences to efficiently disrupt, excise, mutate, insert, or replace genes. The precision of transfection and the level of Cas9 expression should be controlled during the editing processes using specific anti-CRISPR/Cas9 antibodies.</p>
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<h4><strong>Reason #1</strong> Check the transfection success of the Cas9 protein</h4>
<p>Check <strong>Cas9 expression level</strong> using <strong>Western blot</strong> with validated anti-Cas9 antibodies.</p>
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<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-2.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #2</strong> Check that the Cas9 protein was delivered to the nucleus</h4>
<p>Determine the nuclear localization of Cas9 protein via <strong>IF</strong> or <strong>IHC</strong> using validated anti-Cas9 antibodies</p>
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<h4><strong>Reason #3</strong> Check the Cas9 expression level</h4>
<p><strong>Transient systems</strong>: Check that <strong>Cas9 expression</strong> was transient by using <strong>Western blot</strong> and anti-Cas9 antibodies. Prolonged Cas9 expression will lead to more off-target mutations.</p>
<p><strong>Stable transformants</strong>: Isolate several clones and screen to check the level of <strong>Cas9 expression</strong> using <strong>Western blot</strong> and specific anti-Cas9 antibodies. High level of Cas9 expression can lead to non-specific activity.</p>
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<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-4.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #4</strong> Check the binding specificity of Cas9</h4>
<p>Test the binding specificity with an sgRNA of choice by using the <strong>ChIP-grade anti-Cas9 antibody</strong> and the primers for targeted and non-targeted region to see if Cas9 is bound to the correct region.</p>
<p>For more details go to the page <strong><a href="https://www.diagenode.com/en/categories/selection-of-guide-rna-by-ChIP">Selection of guide RNA by ChIP</a></strong>.</p>
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<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>Published examples of the use of anti-Cas9 antibodies:</h3>
<ul>
<li><a href="../publications/view/2823">A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing</a></li>
<li><a href="../publications/view/2792">A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage</a></li>
<li><a href="../publications/view/2765">Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection</a></li>
<li><a href="../publications/view/2679">Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells</a></li>
<li><a href="../publications/view/2645">Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis</a></li>
<li><a href="../publications/view/2961">An inducible lentiviral guide RNA platform enables the identification of tumor-essential genes and tumor-promoting mutations in vivo</a></li>
</ul>
<h5><strong>Diagenode offers the largest collection of validated anti-CRISPR/Cas antibodies, validated in different applications (WB, IF, IP and ChIP).</strong></h5>
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]
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			'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.',
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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			'description' => '<p>The <strong>CRISPR/Cas genome editing system</strong> uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, this technology, can also be used to mediate up- or downregulation of specific endogenous genes or to alter histone modifications or DNA methylation. The best characterized CRISPR-associated nucleases are the <strong>Cas9 proteins</strong> from <em>Streptococcus pyogenes</em> and <em>Staphylococcus aureus</em>. Recently two new CRISPR-associated nucleases, named <strong>Cpf1</strong> (from <em>Lachnospiraceae bacterium</em> and <em>Acidaminococcus sp.</em>), have been described.<br /><br /><span>Diagenode, a dedicated supplier of high quality Cas9 antibodies, was the first company that offered the antibody Cas9 (clone 7A9). </span><span>Diagenode now offers the largest collection of optimized anti-CRISPR/Cas antibodies, validated in a number of different applications including Western blot (WB), immunofluorescence (IF), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP).</span></p>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3><a name="4reasons"></a>Four critical reasons to use anti-CRISPR/Cas9 antibodies</h3>
<p>CRISPR/Cas9 genome editing allows for double-stranded DNA breaks at specific sequences to efficiently disrupt, excise, mutate, insert, or replace genes. The precision of transfection and the level of Cas9 expression should be controlled during the editing processes using specific anti-CRISPR/Cas9 antibodies.</p>
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<h4><strong>Reason #1</strong> Check the transfection success of the Cas9 protein</h4>
<p>Check <strong>Cas9 expression level</strong> using <strong>Western blot</strong> with validated anti-Cas9 antibodies.</p>
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<div class="row">
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<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #2</strong> Check that the Cas9 protein was delivered to the nucleus</h4>
<p>Determine the nuclear localization of Cas9 protein via <strong>IF</strong> or <strong>IHC</strong> using validated anti-Cas9 antibodies</p>
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<h4><strong>Reason #3</strong> Check the Cas9 expression level</h4>
<p><strong>Transient systems</strong>: Check that <strong>Cas9 expression</strong> was transient by using <strong>Western blot</strong> and anti-Cas9 antibodies. Prolonged Cas9 expression will lead to more off-target mutations.</p>
<p><strong>Stable transformants</strong>: Isolate several clones and screen to check the level of <strong>Cas9 expression</strong> using <strong>Western blot</strong> and specific anti-Cas9 antibodies. High level of Cas9 expression can lead to non-specific activity.</p>
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<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #4</strong> Check the binding specificity of Cas9</h4>
<p>Test the binding specificity with an sgRNA of choice by using the <strong>ChIP-grade anti-Cas9 antibody</strong> and the primers for targeted and non-targeted region to see if Cas9 is bound to the correct region.</p>
<p>For more details go to the page <strong><a href="https://www.diagenode.com/en/categories/selection-of-guide-rna-by-ChIP">Selection of guide RNA by ChIP</a></strong>.</p>
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<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>Published examples of the use of anti-Cas9 antibodies:</h3>
<ul>
<li><a href="../publications/view/2823">A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing</a></li>
<li><a href="../publications/view/2792">A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage</a></li>
<li><a href="../publications/view/2765">Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection</a></li>
<li><a href="../publications/view/2679">Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells</a></li>
<li><a href="../publications/view/2645">Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis</a></li>
<li><a href="../publications/view/2961">An inducible lentiviral guide RNA platform enables the identification of tumor-essential genes and tumor-promoting mutations in vivo</a></li>
</ul>
<h5><strong>Diagenode offers the largest collection of validated anti-CRISPR/Cas antibodies, validated in different applications (WB, IF, IP and ChIP).</strong></h5>
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
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ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118