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<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
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<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
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<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
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<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
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<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
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<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
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<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
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<p style="text-align: center;">Yes</p>
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<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
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<td style="text-align: center;">
<p style="text-align: center;">No</p>
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<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
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<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
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<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
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<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
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<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
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<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
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</tbody>
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<h3>Shearing Consumables</h3>
<table style="width: 646px;">
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<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
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<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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'name' => 'tRNA methyltransferase homolog gene TRMT10A mutation in young onset diabetes and primary microcephaly in humans.',
'authors' => 'Igoillo-Esteve M, Genin A, Lambert N, Désir J, Pirson I, Abdulkarim B, Simonis N, Drielsma A, Marselli L, Marchetti P, Vanderhaeghen P, Eizirik DL, Wuyts W, Julier C, Chakera AJ, Ellard S, Hattersley AT, Abramowicz M, Cnop M',
'description' => 'We describe a new syndrome of young onset diabetes, short stature and microcephaly with intellectual disability in a large consanguineous family with three affected children. Linkage analysis and whole exome sequencing were used to identify the causal nonsense mutation, which changed an arginine codon into a stop at position 127 of the tRNA methyltransferase homolog gene TRMT10A (also called RG9MTD2). TRMT10A mRNA and protein were absent in lymphoblasts from the affected siblings. TRMT10A is ubiquitously expressed but enriched in brain and pancreatic islets, consistent with the tissues affected in this syndrome. In situ hybridization studies showed that TRMT10A is expressed in human embryonic and fetal brain. TRMT10A is the mammalian ortholog of S. cerevisiae TRM10, previously shown to catalyze the methylation of guanine 9 (m(1)G9) in several tRNAs. Consistent with this putative function, in silico topology prediction indicated that TRMT10A has predominant nuclear localization, which we experimentally confirmed by immunofluorescence and confocal microscopy. TRMT10A localizes to the nucleolus of β- and non-β-cells, where tRNA modifications occur. TRMT10A silencing induces rat and human β-cell apoptosis. Taken together, we propose that TRMT10A deficiency negatively affects β-cell mass and the pool of neurons in the developing brain. This is the first study describing the impact of TRMT10A deficiency in mammals, highlighting a role in the pathogenesis of microcephaly and early onset diabetes. In light of the recent report that the type 2 diabetes candidate gene CDKAL1 is a tRNA methylthiotransferase, the findings in this family suggest broader relevance of tRNA methyltransferases in the pathogenesis of type 2 diabetes.',
'date' => '2013-10-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/24204302',
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'description' => 'Streptomycete bacteria are renowned as a prolific source of natural products with diverse biological activities. Production of these metabolites is often subject to transcriptional regulation: the biosynthetic genes remain silent until the required environmental and/or physiological signals occur. Consequently, in the laboratory environment, many gene clusters that direct the biosynthesis of natural products with clinical potential are not expressed or at very low level preventing the production/detection of the associated metabolite. Genetic engineering of streptomycetes can unleash the production of many new natural products. This chapter describes the overexpression of pathway-specific activators, the genetic disruption of pathway-specific repressors, and the main strategy used to identify and characterize new natural products from these engineered Streptomyces strains.',
'date' => '0000-00-00',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/23084947',
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<div class="small-12 columns" >
<h6 style="height:60px">Bioruptor® Plus sonication device</h6>
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</div>
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<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
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<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
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'label1' => 'Which Bioruptor® Plus configuration is right for you?',
'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus',
'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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<p><span>Isolation of intact RNA is essential for many techniques used in gene expression analysis (eg. RT-PCR). The RNA extraction kit is a ready-to-use reagent for total RNA extraction from tissues. The RNA extraction kit outperforms standard RNA extraction reagents for RNA extraction from fresh or frozen tissues when used in combination with RNA extraction beads (included in RNA extraction kit) and Bioruptor</span><sup><span>®</span></sup><span> Plus sonication. Efficient disruption and homogenization of the starting material is an absolute requirement for all total RNA purification procedures. Disruption and homogenization of tissue samples can be carried out rapidly and efficiently using the Bioruptor</span><sup><span>® </span></sup><span>Plus</span><span>. </span></p>
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'description' => 'Streptomycete bacteria are renowned as a prolific source of natural products with diverse biological activities. Production of these metabolites is often subject to transcriptional regulation: the biosynthetic genes remain silent until the required environmental and/or physiological signals occur. Consequently, in the laboratory environment, many gene clusters that direct the biosynthesis of natural products with clinical potential are not expressed or at very low level preventing the production/detection of the associated metabolite. Genetic engineering of streptomycetes can unleash the production of many new natural products. This chapter describes the overexpression of pathway-specific activators, the genetic disruption of pathway-specific repressors, and the main strategy used to identify and characterize new natural products from these engineered Streptomyces strains.',
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<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
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<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
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'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
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<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
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<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
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<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
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<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
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<p style="text-align: center;">Yes</p>
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<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
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<td style="text-align: center;">
<p style="text-align: center;">No</p>
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<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
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<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
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<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
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<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
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<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
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</tbody>
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<h3>Shearing Consumables</h3>
<table style="width: 646px;">
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<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
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<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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<div class="large-12 columns">Various biochemical and analytical techniques require the extraction of RNA from tissues. Isolating intact RNA is essential for many techniques used in gene expression analysis. In order to obtain optimal yields of RNA, the efficient disruption and homogenization of tissues and cultured cells are required. <strong>Diagenode's Bioruptor</strong><sup>® </sup><strong>Plus</strong> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) combined with unique devices such as beads or tubes acting as sonication « enhancers » or dedicated reagents (such as our RNA extraction reagent) to efficiently disrupt tissues and cultured cells in just one step to deliver high quality RNA extraction.</div>
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'name' => 'tRNA methyltransferase homolog gene TRMT10A mutation in young onset diabetes and primary microcephaly in humans.',
'authors' => 'Igoillo-Esteve M, Genin A, Lambert N, Désir J, Pirson I, Abdulkarim B, Simonis N, Drielsma A, Marselli L, Marchetti P, Vanderhaeghen P, Eizirik DL, Wuyts W, Julier C, Chakera AJ, Ellard S, Hattersley AT, Abramowicz M, Cnop M',
'description' => 'We describe a new syndrome of young onset diabetes, short stature and microcephaly with intellectual disability in a large consanguineous family with three affected children. Linkage analysis and whole exome sequencing were used to identify the causal nonsense mutation, which changed an arginine codon into a stop at position 127 of the tRNA methyltransferase homolog gene TRMT10A (also called RG9MTD2). TRMT10A mRNA and protein were absent in lymphoblasts from the affected siblings. TRMT10A is ubiquitously expressed but enriched in brain and pancreatic islets, consistent with the tissues affected in this syndrome. In situ hybridization studies showed that TRMT10A is expressed in human embryonic and fetal brain. TRMT10A is the mammalian ortholog of S. cerevisiae TRM10, previously shown to catalyze the methylation of guanine 9 (m(1)G9) in several tRNAs. Consistent with this putative function, in silico topology prediction indicated that TRMT10A has predominant nuclear localization, which we experimentally confirmed by immunofluorescence and confocal microscopy. TRMT10A localizes to the nucleolus of β- and non-β-cells, where tRNA modifications occur. TRMT10A silencing induces rat and human β-cell apoptosis. Taken together, we propose that TRMT10A deficiency negatively affects β-cell mass and the pool of neurons in the developing brain. This is the first study describing the impact of TRMT10A deficiency in mammals, highlighting a role in the pathogenesis of microcephaly and early onset diabetes. In light of the recent report that the type 2 diabetes candidate gene CDKAL1 is a tRNA methylthiotransferase, the findings in this family suggest broader relevance of tRNA methyltransferases in the pathogenesis of type 2 diabetes.',
'date' => '2013-10-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/24204302',
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'description' => 'Streptomycete bacteria are renowned as a prolific source of natural products with diverse biological activities. Production of these metabolites is often subject to transcriptional regulation: the biosynthetic genes remain silent until the required environmental and/or physiological signals occur. Consequently, in the laboratory environment, many gene clusters that direct the biosynthesis of natural products with clinical potential are not expressed or at very low level preventing the production/detection of the associated metabolite. Genetic engineering of streptomycetes can unleash the production of many new natural products. This chapter describes the overexpression of pathway-specific activators, the genetic disruption of pathway-specific repressors, and the main strategy used to identify and characterize new natural products from these engineered Streptomyces strains.',
'date' => '0000-00-00',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/23084947',
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<div class="small-12 columns" >
<h6 style="height:60px">Bioruptor® Plus sonication device</h6>
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</div>
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<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
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<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
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'label1' => 'Which Bioruptor® Plus configuration is right for you?',
'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus',
'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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<p><span>Isolation of intact RNA is essential for many techniques used in gene expression analysis (eg. RT-PCR). The RNA extraction kit is a ready-to-use reagent for total RNA extraction from tissues. The RNA extraction kit outperforms standard RNA extraction reagents for RNA extraction from fresh or frozen tissues when used in combination with RNA extraction beads (included in RNA extraction kit) and Bioruptor</span><sup><span>®</span></sup><span> Plus sonication. Efficient disruption and homogenization of the starting material is an absolute requirement for all total RNA purification procedures. Disruption and homogenization of tissue samples can be carried out rapidly and efficiently using the Bioruptor</span><sup><span>® </span></sup><span>Plus</span><span>. </span></p>
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'description' => 'Streptomycete bacteria are renowned as a prolific source of natural products with diverse biological activities. Production of these metabolites is often subject to transcriptional regulation: the biosynthetic genes remain silent until the required environmental and/or physiological signals occur. Consequently, in the laboratory environment, many gene clusters that direct the biosynthesis of natural products with clinical potential are not expressed or at very low level preventing the production/detection of the associated metabolite. Genetic engineering of streptomycetes can unleash the production of many new natural products. This chapter describes the overexpression of pathway-specific activators, the genetic disruption of pathway-specific repressors, and the main strategy used to identify and characterize new natural products from these engineered Streptomyces strains.',
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<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
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<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
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'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
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<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
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<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
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<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
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<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
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<p style="text-align: center;">Yes</p>
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<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
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<td style="text-align: center;">
<p style="text-align: center;">No</p>
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<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
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<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
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<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
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<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
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<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
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<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
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<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
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</tbody>
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<h3>Shearing Consumables</h3>
<table style="width: 646px;">
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<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
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<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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<div class="large-12 columns">Various biochemical and analytical techniques require the extraction of RNA from tissues. Isolating intact RNA is essential for many techniques used in gene expression analysis. In order to obtain optimal yields of RNA, the efficient disruption and homogenization of tissues and cultured cells are required. <strong>Diagenode's Bioruptor</strong><sup>® </sup><strong>Plus</strong> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) combined with unique devices such as beads or tubes acting as sonication « enhancers » or dedicated reagents (such as our RNA extraction reagent) to efficiently disrupt tissues and cultured cells in just one step to deliver high quality RNA extraction.</div>
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'name' => 'tRNA methyltransferase homolog gene TRMT10A mutation in young onset diabetes and primary microcephaly in humans.',
'authors' => 'Igoillo-Esteve M, Genin A, Lambert N, Désir J, Pirson I, Abdulkarim B, Simonis N, Drielsma A, Marselli L, Marchetti P, Vanderhaeghen P, Eizirik DL, Wuyts W, Julier C, Chakera AJ, Ellard S, Hattersley AT, Abramowicz M, Cnop M',
'description' => 'We describe a new syndrome of young onset diabetes, short stature and microcephaly with intellectual disability in a large consanguineous family with three affected children. Linkage analysis and whole exome sequencing were used to identify the causal nonsense mutation, which changed an arginine codon into a stop at position 127 of the tRNA methyltransferase homolog gene TRMT10A (also called RG9MTD2). TRMT10A mRNA and protein were absent in lymphoblasts from the affected siblings. TRMT10A is ubiquitously expressed but enriched in brain and pancreatic islets, consistent with the tissues affected in this syndrome. In situ hybridization studies showed that TRMT10A is expressed in human embryonic and fetal brain. TRMT10A is the mammalian ortholog of S. cerevisiae TRM10, previously shown to catalyze the methylation of guanine 9 (m(1)G9) in several tRNAs. Consistent with this putative function, in silico topology prediction indicated that TRMT10A has predominant nuclear localization, which we experimentally confirmed by immunofluorescence and confocal microscopy. TRMT10A localizes to the nucleolus of β- and non-β-cells, where tRNA modifications occur. TRMT10A silencing induces rat and human β-cell apoptosis. Taken together, we propose that TRMT10A deficiency negatively affects β-cell mass and the pool of neurons in the developing brain. This is the first study describing the impact of TRMT10A deficiency in mammals, highlighting a role in the pathogenesis of microcephaly and early onset diabetes. In light of the recent report that the type 2 diabetes candidate gene CDKAL1 is a tRNA methylthiotransferase, the findings in this family suggest broader relevance of tRNA methyltransferases in the pathogenesis of type 2 diabetes.',
'date' => '2013-10-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/24204302',
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'description' => 'Streptomycete bacteria are renowned as a prolific source of natural products with diverse biological activities. Production of these metabolites is often subject to transcriptional regulation: the biosynthetic genes remain silent until the required environmental and/or physiological signals occur. Consequently, in the laboratory environment, many gene clusters that direct the biosynthesis of natural products with clinical potential are not expressed or at very low level preventing the production/detection of the associated metabolite. Genetic engineering of streptomycetes can unleash the production of many new natural products. This chapter describes the overexpression of pathway-specific activators, the genetic disruption of pathway-specific repressors, and the main strategy used to identify and characterize new natural products from these engineered Streptomyces strains.',
'date' => '0000-00-00',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/23084947',
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<div class="small-12 columns" >
<h6 style="height:60px">Bioruptor® Plus sonication device</h6>
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<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
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<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
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'label1' => 'Which Bioruptor® Plus configuration is right for you?',
'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus',
'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
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</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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<p><span>Isolation of intact RNA is essential for many techniques used in gene expression analysis (eg. RT-PCR). The RNA extraction kit is a ready-to-use reagent for total RNA extraction from tissues. The RNA extraction kit outperforms standard RNA extraction reagents for RNA extraction from fresh or frozen tissues when used in combination with RNA extraction beads (included in RNA extraction kit) and Bioruptor</span><sup><span>®</span></sup><span> Plus sonication. Efficient disruption and homogenization of the starting material is an absolute requirement for all total RNA purification procedures. Disruption and homogenization of tissue samples can be carried out rapidly and efficiently using the Bioruptor</span><sup><span>® </span></sup><span>Plus</span><span>. </span></p>
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'description' => 'Streptomycete bacteria are renowned as a prolific source of natural products with diverse biological activities. Production of these metabolites is often subject to transcriptional regulation: the biosynthetic genes remain silent until the required environmental and/or physiological signals occur. Consequently, in the laboratory environment, many gene clusters that direct the biosynthesis of natural products with clinical potential are not expressed or at very low level preventing the production/detection of the associated metabolite. Genetic engineering of streptomycetes can unleash the production of many new natural products. This chapter describes the overexpression of pathway-specific activators, the genetic disruption of pathway-specific repressors, and the main strategy used to identify and characterize new natural products from these engineered Streptomyces strains.',
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<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
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<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
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<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
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'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
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<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
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<p style="text-align: center;">< 0.1%</p>
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<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
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<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
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<tr style="background-color: #fff;" valign="middle">
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<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
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<p style="text-align: center;">Yes</p>
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<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
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<td style="text-align: center;">
<p style="text-align: center;">No</p>
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<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
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<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
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<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
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<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
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<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
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<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
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<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
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<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
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<h3>Shearing Consumables</h3>
<table style="width: 646px;">
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<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
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<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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'name' => 'tRNA methyltransferase homolog gene TRMT10A mutation in young onset diabetes and primary microcephaly in humans.',
'authors' => 'Igoillo-Esteve M, Genin A, Lambert N, Désir J, Pirson I, Abdulkarim B, Simonis N, Drielsma A, Marselli L, Marchetti P, Vanderhaeghen P, Eizirik DL, Wuyts W, Julier C, Chakera AJ, Ellard S, Hattersley AT, Abramowicz M, Cnop M',
'description' => 'We describe a new syndrome of young onset diabetes, short stature and microcephaly with intellectual disability in a large consanguineous family with three affected children. Linkage analysis and whole exome sequencing were used to identify the causal nonsense mutation, which changed an arginine codon into a stop at position 127 of the tRNA methyltransferase homolog gene TRMT10A (also called RG9MTD2). TRMT10A mRNA and protein were absent in lymphoblasts from the affected siblings. TRMT10A is ubiquitously expressed but enriched in brain and pancreatic islets, consistent with the tissues affected in this syndrome. In situ hybridization studies showed that TRMT10A is expressed in human embryonic and fetal brain. TRMT10A is the mammalian ortholog of S. cerevisiae TRM10, previously shown to catalyze the methylation of guanine 9 (m(1)G9) in several tRNAs. Consistent with this putative function, in silico topology prediction indicated that TRMT10A has predominant nuclear localization, which we experimentally confirmed by immunofluorescence and confocal microscopy. TRMT10A localizes to the nucleolus of β- and non-β-cells, where tRNA modifications occur. TRMT10A silencing induces rat and human β-cell apoptosis. Taken together, we propose that TRMT10A deficiency negatively affects β-cell mass and the pool of neurons in the developing brain. This is the first study describing the impact of TRMT10A deficiency in mammals, highlighting a role in the pathogenesis of microcephaly and early onset diabetes. In light of the recent report that the type 2 diabetes candidate gene CDKAL1 is a tRNA methylthiotransferase, the findings in this family suggest broader relevance of tRNA methyltransferases in the pathogenesis of type 2 diabetes.',
'date' => '2013-10-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/24204302',
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'description' => 'Streptomycete bacteria are renowned as a prolific source of natural products with diverse biological activities. Production of these metabolites is often subject to transcriptional regulation: the biosynthetic genes remain silent until the required environmental and/or physiological signals occur. Consequently, in the laboratory environment, many gene clusters that direct the biosynthesis of natural products with clinical potential are not expressed or at very low level preventing the production/detection of the associated metabolite. Genetic engineering of streptomycetes can unleash the production of many new natural products. This chapter describes the overexpression of pathway-specific activators, the genetic disruption of pathway-specific repressors, and the main strategy used to identify and characterize new natural products from these engineered Streptomyces strains.',
'date' => '0000-00-00',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/23084947',
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<div class="small-12 columns" >
<h6 style="height:60px">Bioruptor® Plus sonication device</h6>
</div>
</div>
</li>
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<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
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<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
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'label1' => 'Which Bioruptor® Plus configuration is right for you?',
'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus',
'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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<p><span>Isolation of intact RNA is essential for many techniques used in gene expression analysis (eg. RT-PCR). The RNA extraction kit is a ready-to-use reagent for total RNA extraction from tissues. The RNA extraction kit outperforms standard RNA extraction reagents for RNA extraction from fresh or frozen tissues when used in combination with RNA extraction beads (included in RNA extraction kit) and Bioruptor</span><sup><span>®</span></sup><span> Plus sonication. Efficient disruption and homogenization of the starting material is an absolute requirement for all total RNA purification procedures. Disruption and homogenization of tissue samples can be carried out rapidly and efficiently using the Bioruptor</span><sup><span>® </span></sup><span>Plus</span><span>. </span></p>
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