Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'carrousel_order' => array(
(int) 0 => 'Gene editing'
),
'language' => 'cn',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'product' => array(
'Product' => array(
'id' => '2781',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 polyclonal antibody (N-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310260-100',
'old_catalog_number' => '',
'sf_code' => 'C15310260-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:23',
'created' => '2016-01-27 11:34:03',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '470',
'name' => 'S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal)',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation, Recently, the CRISPR/Cas9 from S. aureus (UniProtKB/Swiss-Prot entry J7RUA5) was also shown to be suitable for humane genome editing. The S. aureus Cas9 has the advantage that it’s smaller and therefore easier to transfect cells with, whereas the efficiency and specificity are similar.',
'clonality' => '',
'isotype' => '',
'lot' => 'A2556-001',
'concentration' => 'not determined',
'reactivity' => 'Staphylococcus aureus',
'type' => 'Polyclonal',
'purity' => 'Whole antiserum',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:10,000</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>1 μg/IP</td>
<td>Fig 2</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:1,000</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>',
'storage_conditions' => '',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 's-aureus-crispr-cas9-polyclonal-antibody-n-terminal-c15310260',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-08-03 16:39:23',
'created' => '2016-01-27 11:35:49',
'select_label' => '470 - S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal) (A2556-001 - not determined - Staphylococcus aureus - Whole antiserum - Rabbit)'
),
'Slave' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal'
)
$carrousel_order = array(
(int) 0 => 'Gene editing'
)
$language = 'cn'
$meta_keywords = 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode'
$meta_description = 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.'
$meta_title = 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode'
$product = array(
'Product' => array(
'id' => '2781',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 polyclonal antibody (N-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310260-100',
'old_catalog_number' => '',
'sf_code' => 'C15310260-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:23',
'created' => '2016-01-27 11:34:03',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '470',
'name' => 'S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal)',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation, Recently, the CRISPR/Cas9 from S. aureus (UniProtKB/Swiss-Prot entry J7RUA5) was also shown to be suitable for humane genome editing. The S. aureus Cas9 has the advantage that it’s smaller and therefore easier to transfect cells with, whereas the efficiency and specificity are similar.',
'clonality' => '',
'isotype' => '',
'lot' => 'A2556-001',
'concentration' => 'not determined',
'reactivity' => 'Staphylococcus aureus',
'type' => 'Polyclonal',
'purity' => 'Whole antiserum',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:10,000</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>1 μg/IP</td>
<td>Fig 2</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:1,000</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>',
'storage_conditions' => '',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 's-aureus-crispr-cas9-polyclonal-antibody-n-terminal-c15310260',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-08-03 16:39:23',
'created' => '2016-01-27 11:35:49',
'select_label' => '470 - S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal) (A2556-001 - not determined - Staphylococcus aureus - Whole antiserum - Rabbit)'
),
'Slave' => array(
(int) 0 => array(
'id' => '89',
'name' => 'C15310260',
'product_id' => '2781',
'modified' => '2016-02-18 22:01:02',
'created' => '2016-02-18 22:01:02'
)
),
'Group' => array(
'Group' => array(
'id' => '89',
'name' => 'C15310260',
'product_id' => '2781',
'modified' => '2016-02-18 22:01:02',
'created' => '2016-02-18 22:01:02'
),
'Master' => array(
'id' => '2781',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (N-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310260-100',
'old_catalog_number' => '',
'sf_code' => 'C15310260-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:23',
'created' => '2016-01-27 11:34:03'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(
(int) 0 => array(
'id' => '2779',
'antibody_id' => '472',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the C-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong> <br /> Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310259) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310259-100',
'old_catalog_number' => '',
'sf_code' => 'C15310259-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-c-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(C-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,C-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (C-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:26:50',
'created' => '2016-01-27 11:27:00',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array(
[maximum depth reached]
)
)
),
'Application' => array(
(int) 0 => array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '30',
'position' => '10',
'parent_id' => '40',
'name' => 'IP',
'description' => '<p>Immunoprecipitation</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunoprecipitation',
'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:23:07',
'created' => '2015-07-08 13:46:50',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '107',
'position' => '2',
'parent_id' => '100',
'name' => '<i>S. aureus</i> CRISPR/Cas9 antibodies',
'description' => '<p>Diagenode is pleased to offer the <strong>first antibodies</strong> directed against the <strong>CRISPR/Cas9</strong> nuclease from <strong><em>Staphylococcus aureus</em></strong>.</p>
<h3><em>S. aureus</em> Cas9 monoclonal antibody</h3>
<ul>
<li>Excellent results in <strong>WB</strong>,<strong> IP</strong> and<strong> IF</strong></li>
<li>Raised against <strong>N-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200230-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Western blot analysis using the Diagenode monoclonal antibody directed against <em>S. aureus</em> Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with <em>S. aureus</em> Cas 9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crispr-cas9-monoclonal-antibody" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>N-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 1" /></p>
</div>
<div class="small-7 columns">
<p><small> <strong>Immunofluorescence using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (N-terminal)</strong><br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the <em>S. aureus</em> CRISPR/Cas9 antibody (cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>C-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 1" width="400" height="343" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>IP using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 µg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 µl of the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 µg) is shown in lane 1 and 2.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<table>
<thead>
<tr>
<th>Antibody</th>
<th>WB</th>
<th>IP</th>
<th>IF</th>
<th>Antibody raised against</th>
<th>Specificity</th>
</tr>
</thead>
<tbody>
<tr>
<td><a href="/p/s-aureus-crispr-cas9-monoclonal-antibody"><em>S. aureus</em> CRISPR/Cas9 monoclonal antibody</a></td>
<td>+++</td>
<td>+++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>C-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
</tbody>
</table>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 's-aureus-crispr-cas9',
'cookies_tag_id' => '1',
'meta_keywords' => 'CRISPR/Cas9 polyclonal antibody,staphylococcus aureus,SaCas9,S. aureus,Western blot,Immunoprecipitation,Immunofluorescence ',
'meta_description' => 'Diagenode monoclonal antibody directed against Staphylococcus aureus Cas9 has shown excellent results in Western blot analysis(WB) , Immunoprecipitation(IP) and Immunofluorescence (IF)',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 polyclonal antibody (SaCas9) | Diagenode',
'modified' => '2018-03-29 11:58:38',
'created' => '2016-01-27 14:04:26',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '871',
'name' => 'S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal) tds',
'description' => '<p>Datasheet of the S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal).</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/C15310260-aureus-cas9-N-terminal.pdf',
'slug' => 's-aureus-crispr-cas9-polyclonal-antibody-n-terminal-tds-C15310260',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-01-27 13:53:28',
'created' => '2016-01-27 13:53:28',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '11',
'name' => 'Antibodies you can trust',
'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
'slug' => 'antibodies-you-can-trust-poster',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-01 20:18:31',
'created' => '2015-07-03 16:05:15',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '991',
'name' => ' Accurate QC to optimize CRISPR/Cas9 genome editing specificity',
'description' => '<p>The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease research, drug screening, therapy development, rapid diagnostics, and transcriptional modulation. Although CRISPR/Cas9 enables rapid genome editing, several aspects affect its efficiency and specificity including guide RNA design, delivery methods, and off-targets effects. Diagenode has developed strategies to overcome these common pitfalls and has optimized CRISPR/Cas9 genome editing specificity</p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/CRISPR-Cas9-Poster-Accurate_QC.pdf',
'slug' => 'crispr-cas9-accurate-qc',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-02-12 15:36:31',
'created' => '2018-02-12 13:15:37',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1782',
'name' => 'product/antibodies/cas9-icon.png',
'alt' => 'CRISPR/Cas9 Antibody ',
'modified' => '2020-11-27 07:01:20',
'created' => '2018-03-15 15:53:46',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '3407',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal US en',
'language' => 'en',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-US-en-GHS_2_0.pdf',
'countries' => 'US',
'modified' => '2024-01-16 12:33:20',
'created' => '2024-01-16 12:33:20',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '3401',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal GB en',
'language' => 'en',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2024-01-16 12:31:08',
'created' => '2024-01-16 12:31:08',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '3403',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal ES es',
'language' => 'es',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2024-01-16 12:31:52',
'created' => '2024-01-16 12:31:52',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3402',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal FR fr',
'language' => 'fr',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2024-01-16 12:31:30',
'created' => '2024-01-16 12:31:30',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3406',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal BE fr',
'language' => 'fr',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2024-01-16 12:32:58',
'created' => '2024-01-16 12:32:58',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '3405',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal BE nl',
'language' => 'nl',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-BE-nl-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2024-01-16 12:32:37',
'created' => '2024-01-16 12:32:37',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '3404',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal DE de',
'language' => 'de',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2024-01-16 12:32:14',
'created' => '2024-01-16 12:32:14',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '3408',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal JP ja',
'language' => 'ja',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2024-01-16 12:33:39',
'created' => '2024-01-16 12:33:39',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array(
(int) 0 => array(
'id' => '2782',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (N-terminal) (sample size)',
'description' => '<p><span>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</span></p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '20 µl',
'catalog_number' => 'C15310260-20',
'old_catalog_number' => '',
'sf_code' => 'C15310260-361',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size',
'meta_title' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:47',
'created' => '2016-01-27 11:42:56',
'ProductsGroup' => array(
'id' => '96',
'product_id' => '2782',
'group_id' => '89'
)
)
)
$pro = array(
'id' => '2782',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (N-terminal) (sample size)',
'description' => '<p><span>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</span></p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '20 µl',
'catalog_number' => 'C15310260-20',
'old_catalog_number' => '',
'sf_code' => 'C15310260-361',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size',
'meta_title' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:47',
'created' => '2016-01-27 11:42:56',
'ProductsGroup' => array(
'id' => '96',
'product_id' => '2782',
'group_id' => '89'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/cn/p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal"><img src="/img/product/antibodies/cas9-icon.png" alt="CRISPR/Cas9 Antibody " class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C15310259-100</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2779" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/cn/carts/add/2779" id="CartAdd/2779Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2779" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>将 <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> <i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)</strong> 添加至我的购物车。</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('<i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)',
'C15310259-100',
'580',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">结账</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('<i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)',
'C15310259-100',
'580',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">继续购物</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="s-aureus-crisprcas9-polyclonal-antibody-c-terminal" data-reveal-id="cartModal-2779" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">S. aureus CRISPR/Cas9 polyclonal antibody (C-te...</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '2779',
'antibody_id' => '472',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the C-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong> <br /> Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310259) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310259-100',
'old_catalog_number' => '',
'sf_code' => 'C15310259-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-c-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(C-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,C-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (C-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:26:50',
'created' => '2016-01-27 11:27:00',
'ProductsRelated' => array(
'id' => '3674',
'product_id' => '2781',
'related_id' => '2779'
),
'Image' => array(
(int) 0 => array(
'id' => '1782',
'name' => 'product/antibodies/cas9-icon.png',
'alt' => 'CRISPR/Cas9 Antibody ',
'modified' => '2020-11-27 07:01:20',
'created' => '2018-03-15 15:53:46',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2782',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (N-terminal) (sample size)',
'description' => '<p><span>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</span></p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '20 µl',
'catalog_number' => 'C15310260-20',
'old_catalog_number' => '',
'sf_code' => 'C15310260-361',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size',
'meta_title' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:47',
'created' => '2016-01-27 11:42:56',
'ProductsGroup' => array(
'id' => '96',
'product_id' => '2782',
'group_id' => '89'
)
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'ProductsApplication' => array(
'id' => '4325',
'product_id' => '2781',
'application_id' => '29'
)
)
$slugs = array(
(int) 0 => 'immunofluorescence'
)
$applications = array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'locale' => 'zho'
)
$description = '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>'
$name = 'IF'
$document = array(
'id' => '991',
'name' => ' Accurate QC to optimize CRISPR/Cas9 genome editing specificity',
'description' => '<p>The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease research, drug screening, therapy development, rapid diagnostics, and transcriptional modulation. Although CRISPR/Cas9 enables rapid genome editing, several aspects affect its efficiency and specificity including guide RNA design, delivery methods, and off-targets effects. Diagenode has developed strategies to overcome these common pitfalls and has optimized CRISPR/Cas9 genome editing specificity</p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/CRISPR-Cas9-Poster-Accurate_QC.pdf',
'slug' => 'crispr-cas9-accurate-qc',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-02-12 15:36:31',
'created' => '2018-02-12 13:15:37',
'ProductsDocument' => array(
'id' => '2540',
'product_id' => '2781',
'document_id' => '991'
)
)
$sds = array(
'id' => '3408',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal JP ja',
'language' => 'ja',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2024-01-16 12:33:39',
'created' => '2024-01-16 12:33:39',
'ProductsSafetySheet' => array(
'id' => '5514',
'product_id' => '2781',
'safety_sheet_id' => '3408'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'carrousel_order' => array(
(int) 0 => 'Gene editing'
),
'language' => 'cn',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'product' => array(
'Product' => array(
'id' => '2781',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 polyclonal antibody (N-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310260-100',
'old_catalog_number' => '',
'sf_code' => 'C15310260-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:23',
'created' => '2016-01-27 11:34:03',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '470',
'name' => 'S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal)',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation, Recently, the CRISPR/Cas9 from S. aureus (UniProtKB/Swiss-Prot entry J7RUA5) was also shown to be suitable for humane genome editing. The S. aureus Cas9 has the advantage that it’s smaller and therefore easier to transfect cells with, whereas the efficiency and specificity are similar.',
'clonality' => '',
'isotype' => '',
'lot' => 'A2556-001',
'concentration' => 'not determined',
'reactivity' => 'Staphylococcus aureus',
'type' => 'Polyclonal',
'purity' => 'Whole antiserum',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:10,000</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>1 μg/IP</td>
<td>Fig 2</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:1,000</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>',
'storage_conditions' => '',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 's-aureus-crispr-cas9-polyclonal-antibody-n-terminal-c15310260',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-08-03 16:39:23',
'created' => '2016-01-27 11:35:49',
'select_label' => '470 - S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal) (A2556-001 - not determined - Staphylococcus aureus - Whole antiserum - Rabbit)'
),
'Slave' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal'
)
$carrousel_order = array(
(int) 0 => 'Gene editing'
)
$language = 'cn'
$meta_keywords = 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode'
$meta_description = 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.'
$meta_title = 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode'
$product = array(
'Product' => array(
'id' => '2781',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 polyclonal antibody (N-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310260-100',
'old_catalog_number' => '',
'sf_code' => 'C15310260-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:23',
'created' => '2016-01-27 11:34:03',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '470',
'name' => 'S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal)',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation, Recently, the CRISPR/Cas9 from S. aureus (UniProtKB/Swiss-Prot entry J7RUA5) was also shown to be suitable for humane genome editing. The S. aureus Cas9 has the advantage that it’s smaller and therefore easier to transfect cells with, whereas the efficiency and specificity are similar.',
'clonality' => '',
'isotype' => '',
'lot' => 'A2556-001',
'concentration' => 'not determined',
'reactivity' => 'Staphylococcus aureus',
'type' => 'Polyclonal',
'purity' => 'Whole antiserum',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:10,000</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>1 μg/IP</td>
<td>Fig 2</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:1,000</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>',
'storage_conditions' => '',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 's-aureus-crispr-cas9-polyclonal-antibody-n-terminal-c15310260',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-08-03 16:39:23',
'created' => '2016-01-27 11:35:49',
'select_label' => '470 - S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal) (A2556-001 - not determined - Staphylococcus aureus - Whole antiserum - Rabbit)'
),
'Slave' => array(
(int) 0 => array(
'id' => '89',
'name' => 'C15310260',
'product_id' => '2781',
'modified' => '2016-02-18 22:01:02',
'created' => '2016-02-18 22:01:02'
)
),
'Group' => array(
'Group' => array(
'id' => '89',
'name' => 'C15310260',
'product_id' => '2781',
'modified' => '2016-02-18 22:01:02',
'created' => '2016-02-18 22:01:02'
),
'Master' => array(
'id' => '2781',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (N-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310260-100',
'old_catalog_number' => '',
'sf_code' => 'C15310260-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:23',
'created' => '2016-01-27 11:34:03'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(
(int) 0 => array(
'id' => '2779',
'antibody_id' => '472',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the C-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong> <br /> Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310259) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310259-100',
'old_catalog_number' => '',
'sf_code' => 'C15310259-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-c-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(C-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,C-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (C-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:26:50',
'created' => '2016-01-27 11:27:00',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array(
[maximum depth reached]
)
)
),
'Application' => array(
(int) 0 => array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '30',
'position' => '10',
'parent_id' => '40',
'name' => 'IP',
'description' => '<p>Immunoprecipitation</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunoprecipitation',
'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:23:07',
'created' => '2015-07-08 13:46:50',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '107',
'position' => '2',
'parent_id' => '100',
'name' => '<i>S. aureus</i> CRISPR/Cas9 antibodies',
'description' => '<p>Diagenode is pleased to offer the <strong>first antibodies</strong> directed against the <strong>CRISPR/Cas9</strong> nuclease from <strong><em>Staphylococcus aureus</em></strong>.</p>
<h3><em>S. aureus</em> Cas9 monoclonal antibody</h3>
<ul>
<li>Excellent results in <strong>WB</strong>,<strong> IP</strong> and<strong> IF</strong></li>
<li>Raised against <strong>N-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200230-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Western blot analysis using the Diagenode monoclonal antibody directed against <em>S. aureus</em> Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with <em>S. aureus</em> Cas 9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crispr-cas9-monoclonal-antibody" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>N-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 1" /></p>
</div>
<div class="small-7 columns">
<p><small> <strong>Immunofluorescence using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (N-terminal)</strong><br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the <em>S. aureus</em> CRISPR/Cas9 antibody (cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>C-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 1" width="400" height="343" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>IP using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 µg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 µl of the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 µg) is shown in lane 1 and 2.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<table>
<thead>
<tr>
<th>Antibody</th>
<th>WB</th>
<th>IP</th>
<th>IF</th>
<th>Antibody raised against</th>
<th>Specificity</th>
</tr>
</thead>
<tbody>
<tr>
<td><a href="/p/s-aureus-crispr-cas9-monoclonal-antibody"><em>S. aureus</em> CRISPR/Cas9 monoclonal antibody</a></td>
<td>+++</td>
<td>+++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>C-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
</tbody>
</table>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 's-aureus-crispr-cas9',
'cookies_tag_id' => '1',
'meta_keywords' => 'CRISPR/Cas9 polyclonal antibody,staphylococcus aureus,SaCas9,S. aureus,Western blot,Immunoprecipitation,Immunofluorescence ',
'meta_description' => 'Diagenode monoclonal antibody directed against Staphylococcus aureus Cas9 has shown excellent results in Western blot analysis(WB) , Immunoprecipitation(IP) and Immunofluorescence (IF)',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 polyclonal antibody (SaCas9) | Diagenode',
'modified' => '2018-03-29 11:58:38',
'created' => '2016-01-27 14:04:26',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '871',
'name' => 'S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal) tds',
'description' => '<p>Datasheet of the S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal).</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/C15310260-aureus-cas9-N-terminal.pdf',
'slug' => 's-aureus-crispr-cas9-polyclonal-antibody-n-terminal-tds-C15310260',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-01-27 13:53:28',
'created' => '2016-01-27 13:53:28',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '11',
'name' => 'Antibodies you can trust',
'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
'slug' => 'antibodies-you-can-trust-poster',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-01 20:18:31',
'created' => '2015-07-03 16:05:15',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '991',
'name' => ' Accurate QC to optimize CRISPR/Cas9 genome editing specificity',
'description' => '<p>The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease research, drug screening, therapy development, rapid diagnostics, and transcriptional modulation. Although CRISPR/Cas9 enables rapid genome editing, several aspects affect its efficiency and specificity including guide RNA design, delivery methods, and off-targets effects. Diagenode has developed strategies to overcome these common pitfalls and has optimized CRISPR/Cas9 genome editing specificity</p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/CRISPR-Cas9-Poster-Accurate_QC.pdf',
'slug' => 'crispr-cas9-accurate-qc',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-02-12 15:36:31',
'created' => '2018-02-12 13:15:37',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1782',
'name' => 'product/antibodies/cas9-icon.png',
'alt' => 'CRISPR/Cas9 Antibody ',
'modified' => '2020-11-27 07:01:20',
'created' => '2018-03-15 15:53:46',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '3407',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal US en',
'language' => 'en',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-US-en-GHS_2_0.pdf',
'countries' => 'US',
'modified' => '2024-01-16 12:33:20',
'created' => '2024-01-16 12:33:20',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '3401',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal GB en',
'language' => 'en',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2024-01-16 12:31:08',
'created' => '2024-01-16 12:31:08',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '3403',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal ES es',
'language' => 'es',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2024-01-16 12:31:52',
'created' => '2024-01-16 12:31:52',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3402',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal FR fr',
'language' => 'fr',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2024-01-16 12:31:30',
'created' => '2024-01-16 12:31:30',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3406',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal BE fr',
'language' => 'fr',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2024-01-16 12:32:58',
'created' => '2024-01-16 12:32:58',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '3405',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal BE nl',
'language' => 'nl',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-BE-nl-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2024-01-16 12:32:37',
'created' => '2024-01-16 12:32:37',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '3404',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal DE de',
'language' => 'de',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2024-01-16 12:32:14',
'created' => '2024-01-16 12:32:14',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '3408',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal JP ja',
'language' => 'ja',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2024-01-16 12:33:39',
'created' => '2024-01-16 12:33:39',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array(
(int) 0 => array(
'id' => '2782',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (N-terminal) (sample size)',
'description' => '<p><span>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</span></p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '20 µl',
'catalog_number' => 'C15310260-20',
'old_catalog_number' => '',
'sf_code' => 'C15310260-361',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size',
'meta_title' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:47',
'created' => '2016-01-27 11:42:56',
'ProductsGroup' => array(
'id' => '96',
'product_id' => '2782',
'group_id' => '89'
)
)
)
$pro = array(
'id' => '2782',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (N-terminal) (sample size)',
'description' => '<p><span>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</span></p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '20 µl',
'catalog_number' => 'C15310260-20',
'old_catalog_number' => '',
'sf_code' => 'C15310260-361',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size',
'meta_title' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:47',
'created' => '2016-01-27 11:42:56',
'ProductsGroup' => array(
'id' => '96',
'product_id' => '2782',
'group_id' => '89'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/cn/p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal"><img src="/img/product/antibodies/cas9-icon.png" alt="CRISPR/Cas9 Antibody " class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C15310259-100</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2779" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/cn/carts/add/2779" id="CartAdd/2779Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2779" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>将 <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> <i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)</strong> 添加至我的购物车。</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('<i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)',
'C15310259-100',
'580',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">结账</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('<i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)',
'C15310259-100',
'580',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">继续购物</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="s-aureus-crisprcas9-polyclonal-antibody-c-terminal" data-reveal-id="cartModal-2779" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">S. aureus CRISPR/Cas9 polyclonal antibody (C-te...</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '2779',
'antibody_id' => '472',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the C-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong> <br /> Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310259) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310259-100',
'old_catalog_number' => '',
'sf_code' => 'C15310259-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-c-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(C-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,C-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (C-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:26:50',
'created' => '2016-01-27 11:27:00',
'ProductsRelated' => array(
'id' => '3674',
'product_id' => '2781',
'related_id' => '2779'
),
'Image' => array(
(int) 0 => array(
'id' => '1782',
'name' => 'product/antibodies/cas9-icon.png',
'alt' => 'CRISPR/Cas9 Antibody ',
'modified' => '2020-11-27 07:01:20',
'created' => '2018-03-15 15:53:46',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2782',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (N-terminal) (sample size)',
'description' => '<p><span>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</span></p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '20 µl',
'catalog_number' => 'C15310260-20',
'old_catalog_number' => '',
'sf_code' => 'C15310260-361',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size',
'meta_title' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:47',
'created' => '2016-01-27 11:42:56',
'ProductsGroup' => array(
'id' => '96',
'product_id' => '2782',
'group_id' => '89'
)
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'ProductsApplication' => array(
'id' => '4325',
'product_id' => '2781',
'application_id' => '29'
)
)
$slugs = array(
(int) 0 => 'immunofluorescence'
)
$applications = array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'locale' => 'zho'
)
$description = '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>'
$name = 'IF'
$document = array(
'id' => '991',
'name' => ' Accurate QC to optimize CRISPR/Cas9 genome editing specificity',
'description' => '<p>The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease research, drug screening, therapy development, rapid diagnostics, and transcriptional modulation. Although CRISPR/Cas9 enables rapid genome editing, several aspects affect its efficiency and specificity including guide RNA design, delivery methods, and off-targets effects. Diagenode has developed strategies to overcome these common pitfalls and has optimized CRISPR/Cas9 genome editing specificity</p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/CRISPR-Cas9-Poster-Accurate_QC.pdf',
'slug' => 'crispr-cas9-accurate-qc',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-02-12 15:36:31',
'created' => '2018-02-12 13:15:37',
'ProductsDocument' => array(
'id' => '2540',
'product_id' => '2781',
'document_id' => '991'
)
)
$sds = array(
'id' => '3408',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal JP ja',
'language' => 'ja',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2024-01-16 12:33:39',
'created' => '2024-01-16 12:33:39',
'ProductsSafetySheet' => array(
'id' => '5514',
'product_id' => '2781',
'safety_sheet_id' => '3408'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'carrousel_order' => array(
(int) 0 => 'Gene editing'
),
'language' => 'cn',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'product' => array(
'Product' => array(
'id' => '2781',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 polyclonal antibody (N-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310260-100',
'old_catalog_number' => '',
'sf_code' => 'C15310260-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:23',
'created' => '2016-01-27 11:34:03',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '470',
'name' => 'S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal)',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation, Recently, the CRISPR/Cas9 from S. aureus (UniProtKB/Swiss-Prot entry J7RUA5) was also shown to be suitable for humane genome editing. The S. aureus Cas9 has the advantage that it’s smaller and therefore easier to transfect cells with, whereas the efficiency and specificity are similar.',
'clonality' => '',
'isotype' => '',
'lot' => 'A2556-001',
'concentration' => 'not determined',
'reactivity' => 'Staphylococcus aureus',
'type' => 'Polyclonal',
'purity' => 'Whole antiserum',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:10,000</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>1 μg/IP</td>
<td>Fig 2</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:1,000</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>',
'storage_conditions' => '',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 's-aureus-crispr-cas9-polyclonal-antibody-n-terminal-c15310260',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-08-03 16:39:23',
'created' => '2016-01-27 11:35:49',
'select_label' => '470 - S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal) (A2556-001 - not determined - Staphylococcus aureus - Whole antiserum - Rabbit)'
),
'Slave' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal'
)
$carrousel_order = array(
(int) 0 => 'Gene editing'
)
$language = 'cn'
$meta_keywords = 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode'
$meta_description = 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.'
$meta_title = 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode'
$product = array(
'Product' => array(
'id' => '2781',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 polyclonal antibody (N-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310260-100',
'old_catalog_number' => '',
'sf_code' => 'C15310260-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:23',
'created' => '2016-01-27 11:34:03',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '470',
'name' => 'S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal)',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation, Recently, the CRISPR/Cas9 from S. aureus (UniProtKB/Swiss-Prot entry J7RUA5) was also shown to be suitable for humane genome editing. The S. aureus Cas9 has the advantage that it’s smaller and therefore easier to transfect cells with, whereas the efficiency and specificity are similar.',
'clonality' => '',
'isotype' => '',
'lot' => 'A2556-001',
'concentration' => 'not determined',
'reactivity' => 'Staphylococcus aureus',
'type' => 'Polyclonal',
'purity' => 'Whole antiserum',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:10,000</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>1 μg/IP</td>
<td>Fig 2</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:1,000</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>',
'storage_conditions' => '',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 's-aureus-crispr-cas9-polyclonal-antibody-n-terminal-c15310260',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-08-03 16:39:23',
'created' => '2016-01-27 11:35:49',
'select_label' => '470 - S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal) (A2556-001 - not determined - Staphylococcus aureus - Whole antiserum - Rabbit)'
),
'Slave' => array(
(int) 0 => array(
'id' => '89',
'name' => 'C15310260',
'product_id' => '2781',
'modified' => '2016-02-18 22:01:02',
'created' => '2016-02-18 22:01:02'
)
),
'Group' => array(
'Group' => array(
'id' => '89',
'name' => 'C15310260',
'product_id' => '2781',
'modified' => '2016-02-18 22:01:02',
'created' => '2016-02-18 22:01:02'
),
'Master' => array(
'id' => '2781',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (N-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310260-100',
'old_catalog_number' => '',
'sf_code' => 'C15310260-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:23',
'created' => '2016-01-27 11:34:03'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(
(int) 0 => array(
'id' => '2779',
'antibody_id' => '472',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the C-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong> <br /> Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310259) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310259-100',
'old_catalog_number' => '',
'sf_code' => 'C15310259-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-c-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(C-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,C-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (C-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:26:50',
'created' => '2016-01-27 11:27:00',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array(
[maximum depth reached]
)
)
),
'Application' => array(
(int) 0 => array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '30',
'position' => '10',
'parent_id' => '40',
'name' => 'IP',
'description' => '<p>Immunoprecipitation</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunoprecipitation',
'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:23:07',
'created' => '2015-07-08 13:46:50',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '107',
'position' => '2',
'parent_id' => '100',
'name' => '<i>S. aureus</i> CRISPR/Cas9 antibodies',
'description' => '<p>Diagenode is pleased to offer the <strong>first antibodies</strong> directed against the <strong>CRISPR/Cas9</strong> nuclease from <strong><em>Staphylococcus aureus</em></strong>.</p>
<h3><em>S. aureus</em> Cas9 monoclonal antibody</h3>
<ul>
<li>Excellent results in <strong>WB</strong>,<strong> IP</strong> and<strong> IF</strong></li>
<li>Raised against <strong>N-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200230-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Western blot analysis using the Diagenode monoclonal antibody directed against <em>S. aureus</em> Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with <em>S. aureus</em> Cas 9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crispr-cas9-monoclonal-antibody" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>N-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 1" /></p>
</div>
<div class="small-7 columns">
<p><small> <strong>Immunofluorescence using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (N-terminal)</strong><br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the <em>S. aureus</em> CRISPR/Cas9 antibody (cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>C-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 1" width="400" height="343" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>IP using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 µg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 µl of the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 µg) is shown in lane 1 and 2.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<table>
<thead>
<tr>
<th>Antibody</th>
<th>WB</th>
<th>IP</th>
<th>IF</th>
<th>Antibody raised against</th>
<th>Specificity</th>
</tr>
</thead>
<tbody>
<tr>
<td><a href="/p/s-aureus-crispr-cas9-monoclonal-antibody"><em>S. aureus</em> CRISPR/Cas9 monoclonal antibody</a></td>
<td>+++</td>
<td>+++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>C-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
</tbody>
</table>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 's-aureus-crispr-cas9',
'cookies_tag_id' => '1',
'meta_keywords' => 'CRISPR/Cas9 polyclonal antibody,staphylococcus aureus,SaCas9,S. aureus,Western blot,Immunoprecipitation,Immunofluorescence ',
'meta_description' => 'Diagenode monoclonal antibody directed against Staphylococcus aureus Cas9 has shown excellent results in Western blot analysis(WB) , Immunoprecipitation(IP) and Immunofluorescence (IF)',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 polyclonal antibody (SaCas9) | Diagenode',
'modified' => '2018-03-29 11:58:38',
'created' => '2016-01-27 14:04:26',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '871',
'name' => 'S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal) tds',
'description' => '<p>Datasheet of the S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal).</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/C15310260-aureus-cas9-N-terminal.pdf',
'slug' => 's-aureus-crispr-cas9-polyclonal-antibody-n-terminal-tds-C15310260',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-01-27 13:53:28',
'created' => '2016-01-27 13:53:28',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '11',
'name' => 'Antibodies you can trust',
'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
'slug' => 'antibodies-you-can-trust-poster',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-01 20:18:31',
'created' => '2015-07-03 16:05:15',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '991',
'name' => ' Accurate QC to optimize CRISPR/Cas9 genome editing specificity',
'description' => '<p>The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease research, drug screening, therapy development, rapid diagnostics, and transcriptional modulation. Although CRISPR/Cas9 enables rapid genome editing, several aspects affect its efficiency and specificity including guide RNA design, delivery methods, and off-targets effects. Diagenode has developed strategies to overcome these common pitfalls and has optimized CRISPR/Cas9 genome editing specificity</p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/CRISPR-Cas9-Poster-Accurate_QC.pdf',
'slug' => 'crispr-cas9-accurate-qc',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-02-12 15:36:31',
'created' => '2018-02-12 13:15:37',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1782',
'name' => 'product/antibodies/cas9-icon.png',
'alt' => 'CRISPR/Cas9 Antibody ',
'modified' => '2020-11-27 07:01:20',
'created' => '2018-03-15 15:53:46',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '3407',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal US en',
'language' => 'en',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-US-en-GHS_2_0.pdf',
'countries' => 'US',
'modified' => '2024-01-16 12:33:20',
'created' => '2024-01-16 12:33:20',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '3401',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal GB en',
'language' => 'en',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2024-01-16 12:31:08',
'created' => '2024-01-16 12:31:08',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '3403',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal ES es',
'language' => 'es',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2024-01-16 12:31:52',
'created' => '2024-01-16 12:31:52',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3402',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal FR fr',
'language' => 'fr',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2024-01-16 12:31:30',
'created' => '2024-01-16 12:31:30',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3406',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal BE fr',
'language' => 'fr',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2024-01-16 12:32:58',
'created' => '2024-01-16 12:32:58',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '3405',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal BE nl',
'language' => 'nl',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-BE-nl-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2024-01-16 12:32:37',
'created' => '2024-01-16 12:32:37',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '3404',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal DE de',
'language' => 'de',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2024-01-16 12:32:14',
'created' => '2024-01-16 12:32:14',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '3408',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal JP ja',
'language' => 'ja',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2024-01-16 12:33:39',
'created' => '2024-01-16 12:33:39',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array(
(int) 0 => array(
'id' => '2782',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (N-terminal) (sample size)',
'description' => '<p><span>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</span></p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '20 µl',
'catalog_number' => 'C15310260-20',
'old_catalog_number' => '',
'sf_code' => 'C15310260-361',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size',
'meta_title' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:47',
'created' => '2016-01-27 11:42:56',
'ProductsGroup' => array(
'id' => '96',
'product_id' => '2782',
'group_id' => '89'
)
)
)
$pro = array(
'id' => '2782',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (N-terminal) (sample size)',
'description' => '<p><span>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</span></p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '20 µl',
'catalog_number' => 'C15310260-20',
'old_catalog_number' => '',
'sf_code' => 'C15310260-361',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size',
'meta_title' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:47',
'created' => '2016-01-27 11:42:56',
'ProductsGroup' => array(
'id' => '96',
'product_id' => '2782',
'group_id' => '89'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/cn/p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal"><img src="/img/product/antibodies/cas9-icon.png" alt="CRISPR/Cas9 Antibody " class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C15310259-100</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2779" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/cn/carts/add/2779" id="CartAdd/2779Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2779" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>将 <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> <i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)</strong> 添加至我的购物车。</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('<i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)',
'C15310259-100',
'580',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">结账</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('<i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)',
'C15310259-100',
'580',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">继续购物</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="s-aureus-crisprcas9-polyclonal-antibody-c-terminal" data-reveal-id="cartModal-2779" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">S. aureus CRISPR/Cas9 polyclonal antibody (C-te...</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '2779',
'antibody_id' => '472',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the C-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong> <br /> Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310259) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310259-100',
'old_catalog_number' => '',
'sf_code' => 'C15310259-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-c-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(C-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,C-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (C-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:26:50',
'created' => '2016-01-27 11:27:00',
'ProductsRelated' => array(
'id' => '3674',
'product_id' => '2781',
'related_id' => '2779'
),
'Image' => array(
(int) 0 => array(
'id' => '1782',
'name' => 'product/antibodies/cas9-icon.png',
'alt' => 'CRISPR/Cas9 Antibody ',
'modified' => '2020-11-27 07:01:20',
'created' => '2018-03-15 15:53:46',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2782',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (N-terminal) (sample size)',
'description' => '<p><span>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</span></p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '20 µl',
'catalog_number' => 'C15310260-20',
'old_catalog_number' => '',
'sf_code' => 'C15310260-361',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size',
'meta_title' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:47',
'created' => '2016-01-27 11:42:56',
'ProductsGroup' => array(
'id' => '96',
'product_id' => '2782',
'group_id' => '89'
)
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'ProductsApplication' => array(
'id' => '4325',
'product_id' => '2781',
'application_id' => '29'
)
)
$slugs = array(
(int) 0 => 'immunofluorescence'
)
$applications = array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'locale' => 'zho'
)
$description = '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>'
$name = 'IF'
$document = array(
'id' => '991',
'name' => ' Accurate QC to optimize CRISPR/Cas9 genome editing specificity',
'description' => '<p>The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease research, drug screening, therapy development, rapid diagnostics, and transcriptional modulation. Although CRISPR/Cas9 enables rapid genome editing, several aspects affect its efficiency and specificity including guide RNA design, delivery methods, and off-targets effects. Diagenode has developed strategies to overcome these common pitfalls and has optimized CRISPR/Cas9 genome editing specificity</p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/CRISPR-Cas9-Poster-Accurate_QC.pdf',
'slug' => 'crispr-cas9-accurate-qc',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-02-12 15:36:31',
'created' => '2018-02-12 13:15:37',
'ProductsDocument' => array(
'id' => '2540',
'product_id' => '2781',
'document_id' => '991'
)
)
$sds = array(
'id' => '3408',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal JP ja',
'language' => 'ja',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2024-01-16 12:33:39',
'created' => '2024-01-16 12:33:39',
'ProductsSafetySheet' => array(
'id' => '5514',
'product_id' => '2781',
'safety_sheet_id' => '3408'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'carrousel_order' => array(
(int) 0 => 'Gene editing'
),
'language' => 'cn',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'product' => array(
'Product' => array(
'id' => '2781',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 polyclonal antibody (N-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310260-100',
'old_catalog_number' => '',
'sf_code' => 'C15310260-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:23',
'created' => '2016-01-27 11:34:03',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '470',
'name' => 'S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal)',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation, Recently, the CRISPR/Cas9 from S. aureus (UniProtKB/Swiss-Prot entry J7RUA5) was also shown to be suitable for humane genome editing. The S. aureus Cas9 has the advantage that it’s smaller and therefore easier to transfect cells with, whereas the efficiency and specificity are similar.',
'clonality' => '',
'isotype' => '',
'lot' => 'A2556-001',
'concentration' => 'not determined',
'reactivity' => 'Staphylococcus aureus',
'type' => 'Polyclonal',
'purity' => 'Whole antiserum',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:10,000</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>1 μg/IP</td>
<td>Fig 2</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:1,000</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>',
'storage_conditions' => '',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 's-aureus-crispr-cas9-polyclonal-antibody-n-terminal-c15310260',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-08-03 16:39:23',
'created' => '2016-01-27 11:35:49',
'select_label' => '470 - S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal) (A2556-001 - not determined - Staphylococcus aureus - Whole antiserum - Rabbit)'
),
'Slave' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal'
)
$carrousel_order = array(
(int) 0 => 'Gene editing'
)
$language = 'cn'
$meta_keywords = 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode'
$meta_description = 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.'
$meta_title = 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode'
$product = array(
'Product' => array(
'id' => '2781',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 polyclonal antibody (N-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310260-100',
'old_catalog_number' => '',
'sf_code' => 'C15310260-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:23',
'created' => '2016-01-27 11:34:03',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '470',
'name' => 'S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal)',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation, Recently, the CRISPR/Cas9 from S. aureus (UniProtKB/Swiss-Prot entry J7RUA5) was also shown to be suitable for humane genome editing. The S. aureus Cas9 has the advantage that it’s smaller and therefore easier to transfect cells with, whereas the efficiency and specificity are similar.',
'clonality' => '',
'isotype' => '',
'lot' => 'A2556-001',
'concentration' => 'not determined',
'reactivity' => 'Staphylococcus aureus',
'type' => 'Polyclonal',
'purity' => 'Whole antiserum',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:10,000</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>1 μg/IP</td>
<td>Fig 2</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:1,000</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>',
'storage_conditions' => '',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 's-aureus-crispr-cas9-polyclonal-antibody-n-terminal-c15310260',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-08-03 16:39:23',
'created' => '2016-01-27 11:35:49',
'select_label' => '470 - S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal) (A2556-001 - not determined - Staphylococcus aureus - Whole antiserum - Rabbit)'
),
'Slave' => array(
(int) 0 => array(
'id' => '89',
'name' => 'C15310260',
'product_id' => '2781',
'modified' => '2016-02-18 22:01:02',
'created' => '2016-02-18 22:01:02'
)
),
'Group' => array(
'Group' => array(
'id' => '89',
'name' => 'C15310260',
'product_id' => '2781',
'modified' => '2016-02-18 22:01:02',
'created' => '2016-02-18 22:01:02'
),
'Master' => array(
'id' => '2781',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (N-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310260-100',
'old_catalog_number' => '',
'sf_code' => 'C15310260-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureusCRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:23',
'created' => '2016-01-27 11:34:03'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(
(int) 0 => array(
'id' => '2779',
'antibody_id' => '472',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the C-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong> <br /> Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310259) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310259-100',
'old_catalog_number' => '',
'sf_code' => 'C15310259-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-c-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(C-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,C-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (C-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:26:50',
'created' => '2016-01-27 11:27:00',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array(
[maximum depth reached]
)
)
),
'Application' => array(
(int) 0 => array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '30',
'position' => '10',
'parent_id' => '40',
'name' => 'IP',
'description' => '<p>Immunoprecipitation</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunoprecipitation',
'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:23:07',
'created' => '2015-07-08 13:46:50',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '107',
'position' => '2',
'parent_id' => '100',
'name' => '<i>S. aureus</i> CRISPR/Cas9 antibodies',
'description' => '<p>Diagenode is pleased to offer the <strong>first antibodies</strong> directed against the <strong>CRISPR/Cas9</strong> nuclease from <strong><em>Staphylococcus aureus</em></strong>.</p>
<h3><em>S. aureus</em> Cas9 monoclonal antibody</h3>
<ul>
<li>Excellent results in <strong>WB</strong>,<strong> IP</strong> and<strong> IF</strong></li>
<li>Raised against <strong>N-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200230-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Western blot analysis using the Diagenode monoclonal antibody directed against <em>S. aureus</em> Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with <em>S. aureus</em> Cas 9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crispr-cas9-monoclonal-antibody" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>N-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 1" /></p>
</div>
<div class="small-7 columns">
<p><small> <strong>Immunofluorescence using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (N-terminal)</strong><br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the <em>S. aureus</em> CRISPR/Cas9 antibody (cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>C-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 1" width="400" height="343" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>IP using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 µg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 µl of the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 µg) is shown in lane 1 and 2.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<table>
<thead>
<tr>
<th>Antibody</th>
<th>WB</th>
<th>IP</th>
<th>IF</th>
<th>Antibody raised against</th>
<th>Specificity</th>
</tr>
</thead>
<tbody>
<tr>
<td><a href="/p/s-aureus-crispr-cas9-monoclonal-antibody"><em>S. aureus</em> CRISPR/Cas9 monoclonal antibody</a></td>
<td>+++</td>
<td>+++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>C-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
</tbody>
</table>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 's-aureus-crispr-cas9',
'cookies_tag_id' => '1',
'meta_keywords' => 'CRISPR/Cas9 polyclonal antibody,staphylococcus aureus,SaCas9,S. aureus,Western blot,Immunoprecipitation,Immunofluorescence ',
'meta_description' => 'Diagenode monoclonal antibody directed against Staphylococcus aureus Cas9 has shown excellent results in Western blot analysis(WB) , Immunoprecipitation(IP) and Immunofluorescence (IF)',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 polyclonal antibody (SaCas9) | Diagenode',
'modified' => '2018-03-29 11:58:38',
'created' => '2016-01-27 14:04:26',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '871',
'name' => 'S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal) tds',
'description' => '<p>Datasheet of the S. aureus CRISPR/Cas9 polyclonal antibody (N-terminal).</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/C15310260-aureus-cas9-N-terminal.pdf',
'slug' => 's-aureus-crispr-cas9-polyclonal-antibody-n-terminal-tds-C15310260',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-01-27 13:53:28',
'created' => '2016-01-27 13:53:28',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '11',
'name' => 'Antibodies you can trust',
'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
'slug' => 'antibodies-you-can-trust-poster',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-01 20:18:31',
'created' => '2015-07-03 16:05:15',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '991',
'name' => ' Accurate QC to optimize CRISPR/Cas9 genome editing specificity',
'description' => '<p>The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease research, drug screening, therapy development, rapid diagnostics, and transcriptional modulation. Although CRISPR/Cas9 enables rapid genome editing, several aspects affect its efficiency and specificity including guide RNA design, delivery methods, and off-targets effects. Diagenode has developed strategies to overcome these common pitfalls and has optimized CRISPR/Cas9 genome editing specificity</p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/CRISPR-Cas9-Poster-Accurate_QC.pdf',
'slug' => 'crispr-cas9-accurate-qc',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-02-12 15:36:31',
'created' => '2018-02-12 13:15:37',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1782',
'name' => 'product/antibodies/cas9-icon.png',
'alt' => 'CRISPR/Cas9 Antibody ',
'modified' => '2020-11-27 07:01:20',
'created' => '2018-03-15 15:53:46',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '3407',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal US en',
'language' => 'en',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-US-en-GHS_2_0.pdf',
'countries' => 'US',
'modified' => '2024-01-16 12:33:20',
'created' => '2024-01-16 12:33:20',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '3401',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal GB en',
'language' => 'en',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2024-01-16 12:31:08',
'created' => '2024-01-16 12:31:08',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '3403',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal ES es',
'language' => 'es',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2024-01-16 12:31:52',
'created' => '2024-01-16 12:31:52',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3402',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal FR fr',
'language' => 'fr',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2024-01-16 12:31:30',
'created' => '2024-01-16 12:31:30',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3406',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal BE fr',
'language' => 'fr',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2024-01-16 12:32:58',
'created' => '2024-01-16 12:32:58',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '3405',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal BE nl',
'language' => 'nl',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-BE-nl-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2024-01-16 12:32:37',
'created' => '2024-01-16 12:32:37',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '3404',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal DE de',
'language' => 'de',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2024-01-16 12:32:14',
'created' => '2024-01-16 12:32:14',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '3408',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal JP ja',
'language' => 'ja',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2024-01-16 12:33:39',
'created' => '2024-01-16 12:33:39',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array(
(int) 0 => array(
'id' => '2782',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (N-terminal) (sample size)',
'description' => '<p><span>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</span></p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '20 µl',
'catalog_number' => 'C15310260-20',
'old_catalog_number' => '',
'sf_code' => 'C15310260-361',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size',
'meta_title' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:47',
'created' => '2016-01-27 11:42:56',
'ProductsGroup' => array(
'id' => '96',
'product_id' => '2782',
'group_id' => '89'
)
)
)
$pro = array(
'id' => '2782',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (N-terminal) (sample size)',
'description' => '<p><span>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</span></p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '20 µl',
'catalog_number' => 'C15310260-20',
'old_catalog_number' => '',
'sf_code' => 'C15310260-361',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size',
'meta_title' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:47',
'created' => '2016-01-27 11:42:56',
'ProductsGroup' => array(
'id' => '96',
'product_id' => '2782',
'group_id' => '89'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/cn/p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal"><img src="/img/product/antibodies/cas9-icon.png" alt="CRISPR/Cas9 Antibody " class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C15310259-100</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2779" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/cn/carts/add/2779" id="CartAdd/2779Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2779" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>将 <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> <i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)</strong> 添加至我的购物车。</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('<i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)',
'C15310259-100',
'580',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">结账</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('<i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)',
'C15310259-100',
'580',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">继续购物</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="s-aureus-crisprcas9-polyclonal-antibody-c-terminal" data-reveal-id="cartModal-2779" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">S. aureus CRISPR/Cas9 polyclonal antibody (C-te...</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '2779',
'antibody_id' => '472',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (C-terminal)',
'description' => '<p>Polyclonal antibody raised in rabbit against the C-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong> <br /> Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310259) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 μl',
'catalog_number' => 'C15310259-100',
'old_catalog_number' => '',
'sf_code' => 'C15310259-D001-001161',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-c-terminal',
'meta_title' => 'Staphylococcus aureus CRISPR/Cas9 Polyclonal Antibody(C-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,C-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (C-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:26:50',
'created' => '2016-01-27 11:27:00',
'ProductsRelated' => array(
'id' => '3674',
'product_id' => '2781',
'related_id' => '2779'
),
'Image' => array(
(int) 0 => array(
'id' => '1782',
'name' => 'product/antibodies/cas9-icon.png',
'alt' => 'CRISPR/Cas9 Antibody ',
'modified' => '2020-11-27 07:01:20',
'created' => '2018-03-15 15:53:46',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2782',
'antibody_id' => '470',
'name' => '<i>S. aureus</i> CRISPR/Cas9 Antibody (N-terminal) (sample size)',
'description' => '<p><span>Polyclonal antibody raised in rabbit against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.</span></p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IP.jpg" alt="IP figure 2" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 3" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '20 µl',
'catalog_number' => 'C15310260-20',
'old_catalog_number' => '',
'sf_code' => 'C15310260-361',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 's-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size',
'meta_title' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody(N-terminal) | Diagenode',
'meta_keywords' => 'S. aureus,Cas9,CRISPR, polyclonal antibody,N-terminal,diagenode',
'meta_description' => 'S. aureus CRISPR/Cas9 Polyclonal Antibody (N-terminal) validated in WB, IP and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:34:47',
'created' => '2016-01-27 11:42:56',
'ProductsGroup' => array(
'id' => '96',
'product_id' => '2782',
'group_id' => '89'
)
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'ProductsApplication' => array(
'id' => '4325',
'product_id' => '2781',
'application_id' => '29'
)
)
$slugs = array(
(int) 0 => 'immunofluorescence'
)
$applications = array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'locale' => 'zho'
)
$description = '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>'
$name = 'IF'
$document = array(
'id' => '991',
'name' => ' Accurate QC to optimize CRISPR/Cas9 genome editing specificity',
'description' => '<p>The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease research, drug screening, therapy development, rapid diagnostics, and transcriptional modulation. Although CRISPR/Cas9 enables rapid genome editing, several aspects affect its efficiency and specificity including guide RNA design, delivery methods, and off-targets effects. Diagenode has developed strategies to overcome these common pitfalls and has optimized CRISPR/Cas9 genome editing specificity</p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/CRISPR-Cas9-Poster-Accurate_QC.pdf',
'slug' => 'crispr-cas9-accurate-qc',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-02-12 15:36:31',
'created' => '2018-02-12 13:15:37',
'ProductsDocument' => array(
'id' => '2540',
'product_id' => '2781',
'document_id' => '991'
)
)
$sds = array(
'id' => '3408',
'name' => 'SDS C15310260 S aureus CRISPR Cas9 Antibody N-terminal JP ja',
'language' => 'ja',
'url' => 'files/SDS/S_aureus/SDS-C15310260-S_aureus_CRISPR_Cas9_Antibody_N-terminal_-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2024-01-16 12:33:39',
'created' => '2024-01-16 12:33:39',
'ProductsSafetySheet' => array(
'id' => '5514',
'product_id' => '2781',
'safety_sheet_id' => '3408'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
×
