ELISA Enzyme-linked immunosorbent assay. Read more |
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Lot | A1012-001 |
---|---|
Concentration | not determined |
Species reactivity | Yeast |
Type | Polyclonal |
Purity | Whole antiserum |
Host | Rabbit |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Applications | Suggested dilution | References |
---|---|---|
ELISA | 1:1,000 | Fig 1 |
Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against yeast Smc3K112/113ac (cat. No. CS-PA009-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:16,900.
Datasheet Smc3K112113ac CS-PA009-100 DATASHEET Datasheet description | Download |
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
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Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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Batch-specific data available on the website. Alternative names: BAM, CSPG6, SMC3L1, HCAP, CDLS3' $meta_title = 'Smc3K112/113ac Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2145', 'antibody_id' => '28', 'name' => 'Smc3K112/113ac polyclonal antibody ', 'description' => '<p><span>Alternative names: <strong>BAM</strong>, <strong>CSPG6</strong>, <strong>SMC3L1</strong>, <strong>HCAP</strong>, <strong>CDLS3</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against yeast Smc3 (structural maintenance of chromosomes 3) acetylated at lysines 112 and 113, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310229-Fig1-ELISA.jpg" alt="ELISA" height="329" width="398" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. 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Batch-specific data available on the website. Alternative names: BAM, CSPG6, SMC3L1, HCAP, CDLS3', 'modified' => '2022-01-05 16:08:36', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => '28', 'name' => 'Smc3K112/113ac polyclonal antibody', 'description' => 'SMC3 (UniProtKB/Swiss-Prot entry Q9UQE7) is a central component of the cohesion complex, which is required for chromosome cohesion during the cell cycle. The cohesion complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosome movement. 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$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'cn', 'meta_keywords' => '', 'meta_description' => 'Smc3K112/113ac (Structural maintenance of chromosomes 3) acetylated at lysines 112 and 113) Polyclonal Antibody validated in ELISA. Batch-specific data available on the website. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against yeast Smc3K112/113ac (cat. No. CS-PA009-100). The plates were coated with the peptide used for immunization of the rabbit. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-07-11 14:44:31', 'created' => '0000-00-00 00:00:00', 'select_label' => '28 - Smc3K112/113ac polyclonal antibody (A1012-001 - not determined - Yeast - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'cn' $meta_keywords = '' $meta_description = 'Smc3K112/113ac (Structural maintenance of chromosomes 3) acetylated at lysines 112 and 113) Polyclonal Antibody validated in ELISA. Batch-specific data available on the website. Alternative names: BAM, CSPG6, SMC3L1, HCAP, CDLS3' $meta_title = 'Smc3K112/113ac Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2145', 'antibody_id' => '28', 'name' => 'Smc3K112/113ac polyclonal antibody ', 'description' => '<p><span>Alternative names: <strong>BAM</strong>, <strong>CSPG6</strong>, <strong>SMC3L1</strong>, <strong>HCAP</strong>, <strong>CDLS3</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against yeast Smc3 (structural maintenance of chromosomes 3) acetylated at lysines 112 and 113, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310229-Fig1-ELISA.jpg" alt="ELISA" height="329" width="398" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against yeast Smc3K112/113ac (cat. No. CS-PA009-100). The plates were coated with the peptide used for immunization of the rabbit. 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$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'cn', 'meta_keywords' => '', 'meta_description' => 'Smc3K112/113ac (Structural maintenance of chromosomes 3) acetylated at lysines 112 and 113) Polyclonal Antibody validated in ELISA. Batch-specific data available on the website. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against yeast Smc3K112/113ac (cat. No. CS-PA009-100). The plates were coated with the peptide used for immunization of the rabbit. 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Batch-specific data available on the website. Alternative names: BAM, CSPG6, SMC3L1, HCAP, CDLS3' $meta_title = 'Smc3K112/113ac Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2145', 'antibody_id' => '28', 'name' => 'Smc3K112/113ac polyclonal antibody ', 'description' => '<p><span>Alternative names: <strong>BAM</strong>, <strong>CSPG6</strong>, <strong>SMC3L1</strong>, <strong>HCAP</strong>, <strong>CDLS3</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against yeast Smc3 (structural maintenance of chromosomes 3) acetylated at lysines 112 and 113, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310229-Fig1-ELISA.jpg" alt="ELISA" height="329" width="398" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. 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Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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