SMYD3 polyclonal antibody

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	'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated  in  ChIP-qPCR, ELISA, IHC, IP, WB and IF. Other names: ZMYND1, ZNFN3A1, KMT3E, BA74P14.1',
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			'name' => 'SMYD3 polyclonal antibody ',
			'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p>
<p>Polyclonal antibody raised in rabbit against SMYD3 (SET and MYND domain containing 3), using a recombinant protein.</p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 &micro;g/IP) was used as negative IP control. QPCR was performed with primers for the 5&rsquo; flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p>
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<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-9 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 &micro;g) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 &micro;g) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p>
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			'slug' => 'smyd3-polyclonal-antibody-classic-100-mg',
			'meta_title' => 'SMYD3 Polyclonal Antibody | Diagenode',
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			'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated  in  ChIP-qPCR, ELISA, IHC, IP, WB and IF. Other names: ZMYND1, ZNFN3A1, KMT3E, BA74P14.1',
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			'id' => '424',
			'name' => 'SMYD3 polyclonal antibody',
			'description' => 'SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It
is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex
and hence plays an important role in transcriptional activation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => '41906',
			'concentration' => '1.32 μg/μl',
			'reactivity' => 'Human, mouse, rat: positive. Other species: not tested.',
			'type' => 'Polyclonal',
			'purity' => 'Affinity purified polyclonal antibody in PBS containing 20% glycerol and 0.025% ProClin 300.',
			'classification' => '',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution <sup>*</sup></th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP*</td>
<td>5 &micro;g/ChIP</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western blotting</td>
<td>1:500 - 1:1,000</td>
<td>Fig 2, 3</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>5 &micro;g/IP</td>
<td>Fig 4</td>
</tr>
<tr>
<td>IF</td>
<td>1:500</td>
<td>Fig 5</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &micro;g per IP.</small></p>',
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			'modified' => '2018-05-04 15:39:16',
			'created' => '2015-07-28 12:39:00',
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$meta_title = 'SMYD3 Polyclonal Antibody | Diagenode'
$product = array(
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		'id' => '2360',
		'antibody_id' => '424',
		'name' => 'SMYD3 polyclonal antibody ',
		'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p>
<p>Polyclonal antibody raised in rabbit against SMYD3 (SET and MYND domain containing 3), using a recombinant protein.</p>',
		'label1' => 'Validation Data',
		'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 &micro;g/IP) was used as negative IP control. QPCR was performed with primers for the 5&rsquo; flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 &micro;g) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 &micro;g) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p>
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		'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.</p>',
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		'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated  in  ChIP-qPCR, ELISA, IHC, IP, WB and IF. Other names: ZMYND1, ZNFN3A1, KMT3E, BA74P14.1',
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		'description' => 'SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It
is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex
and hence plays an important role in transcriptional activation.',
		'clonality' => '',
		'isotype' => '',
		'lot' => '41906',
		'concentration' => '1.32 μg/μl',
		'reactivity' => 'Human, mouse, rat: positive. Other species: not tested.',
		'type' => 'Polyclonal',
		'purity' => 'Affinity purified polyclonal antibody in PBS containing 20% glycerol and 0.025% ProClin 300.',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution <sup>*</sup></th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP*</td>
<td>5 &micro;g/ChIP</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western blotting</td>
<td>1:500 - 1:1,000</td>
<td>Fig 2, 3</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>5 &micro;g/IP</td>
<td>Fig 4</td>
</tr>
<tr>
<td>IF</td>
<td>1:500</td>
<td>Fig 5</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &micro;g per IP.</small></p>',
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			'name' => 'SMYD3 Antibody',
			'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p>
<p>Polyclonal antibody raised in rabbit against <strong>SMYD3 (SET and MYND domain containing 3)</strong>, using a recombinant protein.</p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 &micro;g/IP) was used as negative IP control. QPCR was performed with primers for the 5&rsquo; flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 &micro;g) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 &micro;g) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p>
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
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			'name' => 'SMYD3: a new regulator of the early steps of adipocyte differentiation',
			'authors' => 'Gilardi  F. et al.',
			'description' => '<p>In obesity, adipose tissue undergoes a remodeling process characterized by increased adipocyte size (hypertrophia) and number (hyperplasia). The individual ability to tip the balance toward the hyperplastic growth, with recruitment of new fat cells through adipogenesis, seems to be critical for a healthy adipose tissue expansion, as opposed to the development of inflammation and detrimental metabolic consequences. However, the molecular mechanisms underlying this fine-tuned regulation are far from being understood. Methods We analyzed by mass spectrometry-based proteomics visceral white adipose tissue (vWAT) samples collected from C57BL6 mice fed with a HFD for 8 weeks. A subset of these mice, called low inflammation (Low-INFL), showed a low susceptibility to the onset of adipose tissue inflammation, as opposed to those developing the expected inflammatory response (Hi-INFL). We identified the discriminants between Low-INFL and Hi-INFL vWAT samples and explored their function in Adipose Derived human Mesenchymal Stem Cells (AD-hMSCs) differentiated to adipocytes. Results We quantified 6051 proteins. Among the candidates that most differentiate Low-INFL from Hi-INFL vWAT, we found proteins involved in adipocyte function, including adiponectin and hormone sensitive lipase, suggesting that adipocyte differentiation is enhanced in Low-INFL, as compared to Hi-INFL. The chromatin modifier SET and MYND Domain Containing 3 (SMYD3), whose function in adipose tissue was so far unknown, was another top-scored hit. SMYD3 expression was significantly higher in Low-INFL vWAT, as confirmed by western blot analysis. In vitro, we found that SMYD3 mRNA and protein levels decrease rapidly along the differentiation process of AD-hMSCs. Moreover, SMYD3 knock-down at the beginning of adipocyte differentiation resulted in reduced cell proliferation and, at longer term, reduced lipid accumulation in adipocytes. Conclusions Our study describes an important role for SMYD3 as a newly discovered regulator of adipocyte proliferation during the early steps of adipogenesis.</p>',
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	'description' => '<p>In obesity, adipose tissue undergoes a remodeling process characterized by increased adipocyte size (hypertrophia) and number (hyperplasia). The individual ability to tip the balance toward the hyperplastic growth, with recruitment of new fat cells through adipogenesis, seems to be critical for a healthy adipose tissue expansion, as opposed to the development of inflammation and detrimental metabolic consequences. However, the molecular mechanisms underlying this fine-tuned regulation are far from being understood. Methods We analyzed by mass spectrometry-based proteomics visceral white adipose tissue (vWAT) samples collected from C57BL6 mice fed with a HFD for 8 weeks. A subset of these mice, called low inflammation (Low-INFL), showed a low susceptibility to the onset of adipose tissue inflammation, as opposed to those developing the expected inflammatory response (Hi-INFL). We identified the discriminants between Low-INFL and Hi-INFL vWAT samples and explored their function in Adipose Derived human Mesenchymal Stem Cells (AD-hMSCs) differentiated to adipocytes. Results We quantified 6051 proteins. Among the candidates that most differentiate Low-INFL from Hi-INFL vWAT, we found proteins involved in adipocyte function, including adiponectin and hormone sensitive lipase, suggesting that adipocyte differentiation is enhanced in Low-INFL, as compared to Hi-INFL. The chromatin modifier SET and MYND Domain Containing 3 (SMYD3), whose function in adipose tissue was so far unknown, was another top-scored hit. SMYD3 expression was significantly higher in Low-INFL vWAT, as confirmed by western blot analysis. In vitro, we found that SMYD3 mRNA and protein levels decrease rapidly along the differentiation process of AD-hMSCs. Moreover, SMYD3 knock-down at the beginning of adipocyte differentiation resulted in reduced cell proliferation and, at longer term, reduced lipid accumulation in adipocytes. Conclusions Our study describes an important role for SMYD3 as a newly discovered regulator of adipocyte proliferation during the early steps of adipogenesis.</p>',
	'date' => '2023-06-01',
	'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-3126142%2Fv1',
	'doi' => '10.21203/rs.3.rs-3126142/v1',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]
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			'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p>
<p>Polyclonal antibody raised in rabbit against SMYD3 (SET and MYND domain containing 3), using a recombinant protein.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 &micro;g/IP) was used as negative IP control. QPCR was performed with primers for the 5&rsquo; flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 &micro;g) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 &micro;g) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 &micro;g/IP) was used as negative IP control. QPCR was performed with primers for the 5&rsquo; flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 &micro;g) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 &micro;g) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p>
</div>
</div>',
		'label2' => 'Target Description',
		'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.</p>',
		'label3' => '',
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		'format' => '100 μl',
		'catalog_number' => 'C15410253-100',
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		'sf_code' => 'C15410253-D001-001161',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '395',
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		'price_GBP' => '345',
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		'last_datasheet_update' => 'January 9, 2017',
		'slug' => 'smyd3-polyclonal-antibody-classic-100-mg',
		'meta_title' => 'SMYD3 Polyclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated  in  ChIP-qPCR, ELISA, IHC, IP, WB and IF. Other names: ZMYND1, ZNFN3A1, KMT3E, BA74P14.1',
		'modified' => '2022-01-05 15:04:59',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'zho'
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	'Antibody' => array(
		'host' => '*****',
		'id' => '424',
		'name' => 'SMYD3 polyclonal antibody',
		'description' => 'SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It
is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex
and hence plays an important role in transcriptional activation.',
		'clonality' => '',
		'isotype' => '',
		'lot' => '41906',
		'concentration' => '1.32 μg/μl',
		'reactivity' => 'Human, mouse, rat: positive. Other species: not tested.',
		'type' => 'Polyclonal',
		'purity' => 'Affinity purified polyclonal antibody in PBS containing 20% glycerol and 0.025% ProClin 300.',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution <sup>*</sup></th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP*</td>
<td>5 &micro;g/ChIP</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western blotting</td>
<td>1:500 - 1:1,000</td>
<td>Fig 2, 3</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>5 &micro;g/IP</td>
<td>Fig 4</td>
</tr>
<tr>
<td>IF</td>
<td>1:500</td>
<td>Fig 5</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &micro;g per IP.</small></p>',
		'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => '',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2018-05-04 15:39:16',
		'created' => '2015-07-28 12:39:00',
		'select_label' => '424 - SMYD3 polyclonal antibody (41906 - 1.32 μg/μl - Human, mouse, rat: positive. Other species: not tested. - Affinity purified polyclonal antibody in PBS containing 20% glycerol and 0.025% ProClin 300. - Rabbit)'
	),
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			'name' => 'SMYD3 Antibody',
			'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p>
<p>Polyclonal antibody raised in rabbit against <strong>SMYD3 (SET and MYND domain containing 3)</strong>, using a recombinant protein.</p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 &micro;g/IP) was used as negative IP control. QPCR was performed with primers for the 5&rsquo; flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p>
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<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 &micro;g) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 &micro;g) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
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<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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<li>Batch-specific data is available on the website</li>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 &micro;g) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p>
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<p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 &micro;g/IP) was used as negative IP control. QPCR was performed with primers for the 5&rsquo; flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 &micro;g) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 &micro;g) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p>
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<p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 &micro;g/IP) was used as negative IP control. QPCR was performed with primers for the 5&rsquo; flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 &micro;g) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 &micro;g) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p>
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<p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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	'name' => 'SMYD3: a new regulator of the early steps of adipocyte differentiation',
	'authors' => 'Gilardi  F. et al.',
	'description' => '<p>In obesity, adipose tissue undergoes a remodeling process characterized by increased adipocyte size (hypertrophia) and number (hyperplasia). The individual ability to tip the balance toward the hyperplastic growth, with recruitment of new fat cells through adipogenesis, seems to be critical for a healthy adipose tissue expansion, as opposed to the development of inflammation and detrimental metabolic consequences. However, the molecular mechanisms underlying this fine-tuned regulation are far from being understood. Methods We analyzed by mass spectrometry-based proteomics visceral white adipose tissue (vWAT) samples collected from C57BL6 mice fed with a HFD for 8 weeks. A subset of these mice, called low inflammation (Low-INFL), showed a low susceptibility to the onset of adipose tissue inflammation, as opposed to those developing the expected inflammatory response (Hi-INFL). We identified the discriminants between Low-INFL and Hi-INFL vWAT samples and explored their function in Adipose Derived human Mesenchymal Stem Cells (AD-hMSCs) differentiated to adipocytes. Results We quantified 6051 proteins. Among the candidates that most differentiate Low-INFL from Hi-INFL vWAT, we found proteins involved in adipocyte function, including adiponectin and hormone sensitive lipase, suggesting that adipocyte differentiation is enhanced in Low-INFL, as compared to Hi-INFL. The chromatin modifier SET and MYND Domain Containing 3 (SMYD3), whose function in adipose tissue was so far unknown, was another top-scored hit. SMYD3 expression was significantly higher in Low-INFL vWAT, as confirmed by western blot analysis. In vitro, we found that SMYD3 mRNA and protein levels decrease rapidly along the differentiation process of AD-hMSCs. Moreover, SMYD3 knock-down at the beginning of adipocyte differentiation resulted in reduced cell proliferation and, at longer term, reduced lipid accumulation in adipocytes. Conclusions Our study describes an important role for SMYD3 as a newly discovered regulator of adipocyte proliferation during the early steps of adipogenesis.</p>',
	'date' => '2023-06-01',
	'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-3126142%2Fv1',
	'doi' => '10.21203/rs.3.rs-3126142/v1',
	'modified' => '2023-08-01 14:00:06',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]
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<p>Polyclonal antibody raised in rabbit against SMYD3 (SET and MYND domain containing 3), using a recombinant protein.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 &micro;g/IP) was used as negative IP control. QPCR was performed with primers for the 5&rsquo; flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 &micro;g) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 &micro;g) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p>
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<td>ChIP*</td>
<td>5 &micro;g/ChIP</td>
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<td>1:500 - 1:1,000</td>
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<td>Immunoprecipitation</td>
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<td>Fig 4</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &micro;g per IP.</small></p>',
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			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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			'modified' => '2018-05-04 15:39:16',
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<p>Polyclonal antibody raised in rabbit against SMYD3 (SET and MYND domain containing 3), using a recombinant protein.</p>',
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 &micro;g/IP) was used as negative IP control. QPCR was performed with primers for the 5&rsquo; flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 &micro;g) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 &micro;g) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p>
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<p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p>
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		'label2' => 'Target Description',
		'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.</p>',
		'label3' => '',
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		'format' => '100 μl',
		'catalog_number' => 'C15410253-100',
		'old_catalog_number' => '',
		'sf_code' => 'C15410253-D001-001161',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '395',
		'price_USD' => '410',
		'price_GBP' => '345',
		'price_JPY' => '61875',
		'price_CNY' => '',
		'price_AUD' => '1025',
		'country' => 'ALL',
		'except_countries' => 'None',
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		'slug' => 'smyd3-polyclonal-antibody-classic-100-mg',
		'meta_title' => 'SMYD3 Polyclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated  in  ChIP-qPCR, ELISA, IHC, IP, WB and IF. Other names: ZMYND1, ZNFN3A1, KMT3E, BA74P14.1',
		'modified' => '2022-01-05 15:04:59',
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	'Antibody' => array(
		'host' => '*****',
		'id' => '424',
		'name' => 'SMYD3 polyclonal antibody',
		'description' => 'SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It
is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex
and hence plays an important role in transcriptional activation.',
		'clonality' => '',
		'isotype' => '',
		'lot' => '41906',
		'concentration' => '1.32 μg/μl',
		'reactivity' => 'Human, mouse, rat: positive. Other species: not tested.',
		'type' => 'Polyclonal',
		'purity' => 'Affinity purified polyclonal antibody in PBS containing 20% glycerol and 0.025% ProClin 300.',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution <sup>*</sup></th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP*</td>
<td>5 &micro;g/ChIP</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western blotting</td>
<td>1:500 - 1:1,000</td>
<td>Fig 2, 3</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>5 &micro;g/IP</td>
<td>Fig 4</td>
</tr>
<tr>
<td>IF</td>
<td>1:500</td>
<td>Fig 5</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &micro;g per IP.</small></p>',
		'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => '',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2018-05-04 15:39:16',
		'created' => '2015-07-28 12:39:00',
		'select_label' => '424 - SMYD3 polyclonal antibody (41906 - 1.32 μg/μl - Human, mouse, rat: positive. Other species: not tested. - Affinity purified polyclonal antibody in PBS containing 20% glycerol and 0.025% ProClin 300. - Rabbit)'
	),
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			'name' => 'SMYD3 Antibody',
			'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p>
<p>Polyclonal antibody raised in rabbit against <strong>SMYD3 (SET and MYND domain containing 3)</strong>, using a recombinant protein.</p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 &micro;g/IP) was used as negative IP control. QPCR was performed with primers for the 5&rsquo; flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 &micro;g) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 &micro;g) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 &micro;g of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p>
</div>
</div>',
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
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<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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