| Tagmentase Dilution Buffer Datasheet DATASHEET Diagenode Tagmentase Dilution Buffer is recommended for dilution of Tagmentase (Cat. No. C0107001... | Download |
Tagmentase Dilution Buffer
Hologic Diagenode Tagmentase Dilution Buffer is recommended for dilution of Tagmentase (Cat. No. C01070010) before or after transposome assembly.
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Benchmarking of duplex sequencing approaches to reveal somatic mutation landscapes
Zhang, Yang et al.
Detecting somatic mutations in normal tissues is challenging due to sequencing errors and the low allele fractions of post-zygotic variants. Duplex sequencing greatly reduces errors and can detect mutations at any allele fraction, but systematic, cross-platform comparisons are lacking. We present a comprehensive...Human iPSCs-based modeling unveils SETBP1 as a driver of chromatin rewiring in GATA2 deficiency
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Patients with GATA2 deficiency are predisposed to developing myelodysplastic neoplasms (MDS), which can progress to acute myeloid leukemia. This progression is often associated with cytogenetic and somatic alterations. Mutations in SETBP1 and ASXL1 genes are recurrently observed in GATA2 patients, although their...Spatial transcriptomic imaging of an intact organism using volumetric DNA microscopy
Qian, N., Weinstein, J.A.
Lymphatic, nervous and tumor tissues exhibit complex physiology arising from three-dimensional interactions within genetically unique microenvironments. Here we develop a technology capable of volumetrically imaging transcriptomes, genotypes and morphologies in a single measurement, without relying on prior knowledg...Recurrent patterns of widespread neuronal genomic damage shared by major neurodegenerative disorders
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Amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), and Alzheimer's disease (AD) are common neurodegenerative disorders for which the mechanisms driving neuronal death remain unclear. Single-cell whole-genome sequencing of 429 neurons from three C9ORF72 ALS, six C9ORF72 FTD, seven AD, and twenty-thre...Enhancing single-cell ATAC sequencing with formaldehyde fixation, cryopreservation, and multiplexing for flexible analysis
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The assay for transposase-accessible chromatin using sequencing (ATAC-seq) revolutionized the field of epigenetics since its emergence by providing a means to uncover chromatin dynamics and other factors affecting gene expression. The development of single-cell (sc) applications in recent years led to an even deeper...Tn5-Duplex-Sequencing (Tn5-Duplex-Seq) for low-input single-molecule variant detection
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DNA mutations are the inevitable consequences of errors that arise during replication-repair of DNA damage as well as aging and disease progression. Because of their random and infrequent occurrence, quantification, and characterization of DNA mutations in the genome of somatic cells have been difficult. These mut...Volumetric imaging of an intact organism by a distributed molecular network
Nianchao Qian and Joshua A Weinstein
Lymphatic, nervous, and tumoral tissues, among others, exhibit physiology that emerges from three-dimensional interactions between genetically unique cells. A technology capable of volumetrically imaging transcriptomes, genotypes, and morphologies in a single de novo measurement would therefore provide a critical vi...