中文 | | | / 搜索

Tagmentation Buffer (2x)

目录号

格式

价格

C01019043-5000

5000 µl

$940.00

其他格式

C01019043

300 µl

C01019043-100

100 μl

C01019043-1000

1000 µl

C01019043-10000

10000 µl

Diagenode Tagmentation Buffer (2x) is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode Tagmentase (Tn5 transposase) on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.

Examples of use
ATAC-seq experiments:
- After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:
Tagmentation Buffer (2x) 25 µl

Tagmentase loaded 2.5 µl

Digitonin 1% 0.5 µl

Tween20 10% 0.5 µl

PBS 16.5 µl

Nuclease-free water 5 µl

Nuclei pellet*

* The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.
- The reaction is then incubated 30 minutes at 37°C.
- The tagmentation reaction can then be stopped by addition of 250 µl of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).
- The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.
文档
Datasheet Tagmentation Buffer (2x) C01019043 DATASHEET
Download

出版物

How to properly cite this product in your work

Diagenode strongly recommends using this: Tagmentation Buffer (2x) (Diagenode Cat# C01019043-5000). Click here to copy to clipboard.

Using our products in your publication? Let us know!

HIRA protects telomeres against R-loop-induced instability in ALT cancer cells
Michelle Lee Lynskey et al.
Highlights HIRA establishes greater telomeric chromatin accessibility after ATRX-DAXX loss Deposition of new H3.3 by HIRA-UBN restricts telomeric ssDNA and TERRA R-loops Unresolved TERRA R-loops block new H3.3 deposition by HIRA-UBN CHK1 phosphorylation of H3.3 is critical to pr...

HNF1β bookmarking involves Topoisomerase 1 activation and DNA topology relaxation in mitotic chromatin
Alessia Bagattin et al.
Highlights HNF1β mitotic site binding is preserved with a specific methanol/formaldehyde ChIP BTBD2, an HNF1β partner, mediates mitosis-specific interaction with TOP1 HNF1β recruits TOP1 and induces DNA relaxation around bookmarked HNF1β sites An HNF1β m...

High-throughput sequencing of insect specimens with sub-optimal DNA preservation using a practical, plate-based Illumina-compatible Tn5 transposase library preparation method
Cobb L. et all.
Entomological sampling and storage conditions often prioritise efficiency, practicality and conservation of morphological characteristics, and may therefore be suboptimal for DNA preservation. This practice can impact downstream molecular applications, such as the generation of high-throughput genomic libraries, whi...

On the identification of differentially-active transcription factors from ATAC-seq data
Gerbaldo F. et al.
ATAC-seq has emerged as a rich epigenome profiling technique, and is commonly used to identify Transcription Factors (TFs) underlying given phenomena. A number of methods can be used to identify differentially-active TFs through the accessibility of their DNA-binding motif, however little is known on the best approa...

ARID1A governs the silencing of sex-linked transcription during male meiosis in the mouse
Menon D.U. et al.
We present evidence implicating the BAF (BRG1/BRM Associated Factor) chromatin remodeler in meiotic sex chromosome inactivation (MSCI). By immunofluorescence (IF), the putative BAF DNA binding subunit, ARID1A (AT-rich Interaction Domain 1a), appeared enriched on the male sex chromosomes during diplonema of meiosis I...

YAP/BRD4-controlled ROR1 promotes tumor-initiating cells andhyperproliferation in pancreatic cancer.
Yamazaki M. et al.
Tumor-initiating cells are major drivers of chemoresistance and attractive targets for cancer therapy, however, their identity in human pancreatic ductal adenocarcinoma (PDAC) and the key molecules underlying their traits remain poorly understood. Here, we show that a cellular subpopulation with partial epithelial-m...

A neurodevelopmental epigenetic programme mediated bySMARCD3-DAB1-Reelin signalling is hijacked to promote medulloblastomametastasis.
Zou Han et al.
How abnormal neurodevelopment relates to the tumour aggressiveness of medulloblastoma (MB), the most common type of embryonal tumour, remains elusive. Here we uncover a neurodevelopmental epigenomic programme that is hijacked to induce MB metastatic dissemination. Unsupervised analyses of integrated publicly availab...

EBF1 is continuously required for stabilizing local chromatinaccessibility in pro-B cells.
Zolotarev Nikolay et al.
The establishment of de novo chromatin accessibility in lymphoid progenitors requires the "pioneering" function of transcription factor (TF) early B cell factor 1 (EBF1), which binds to naïve chromatin and induces accessibility by recruiting the BRG1 chromatin remodeler subunit. However, it remains unclear whet...

Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'cn',
	'meta_keywords' => '',
	'meta_description' => '',
	'meta_title' => 'Tagmentation Buffer 2x',
	'product' => array(
		'Product' => array(
			'id' => '3228',
			'antibody_id' => null,
			'name' => 'Tagmentation Buffer (2x)',
			'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
			'label1' => 'Examples of use',
			'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '5000 µl',
			'catalog_number' => 'C01019043-5000',
			'old_catalog_number' => '',
			'sf_code' => 'C01019043-190330',
			'type' => 'FRE',
			'search_order' => '',
			'price_EUR' => '845',
			'price_USD' => '940',
			'price_GBP' => '990',
			'price_JPY' => '132370',
			'price_CNY' => '',
			'price_AUD' => '2350',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => false,
			'last_datasheet_update' => '',
			'slug' => 'tagmentation-buffer-2x-5000',
			'meta_title' => 'Tagmentation Buffer 2x',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-11-25 12:27:51',
			'created' => '2022-11-25 11:18:25',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(),
		'Group' => array(
			'Group' => array(
				[maximum depth reached]
			),
			'Master' => array(
				[maximum depth reached]
			),
			'Product' => array(
				[maximum depth reached]
			)
		),
		'Related' => array(),
		'Application' => array(),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	),
	'meta_canonical' => 'https://www.diagenode.com/cn/p/tagmentation-buffer-2x'
)
$language = 'cn'
$meta_keywords = ''
$meta_description = ''
$meta_title = 'Tagmentation Buffer 2x'
$product = array(
	'Product' => array(
		'id' => '3228',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
		'label1' => 'Examples of use',
		'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label3' => '',
		'info3' => '',
		'format' => '5000 µl',
		'catalog_number' => 'C01019043-5000',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-190330',
		'type' => 'FRE',
		'search_order' => '',
		'price_EUR' => '845',
		'price_USD' => '940',
		'price_GBP' => '990',
		'price_JPY' => '132370',
		'price_CNY' => '',
		'price_AUD' => '2350',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x-5000',
		'meta_title' => 'Tagmentation Buffer 2x',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2022-11-25 12:27:51',
		'created' => '2022-11-25 11:18:25',
		'locale' => 'zho'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(),
	'Group' => array(
		'Group' => array(
			'id' => '330',
			'name' => 'C01019043',
			'product_id' => '3162',
			'modified' => '2022-11-25 11:27:26',
			'created' => '2020-12-08 16:06:34'
		),
		'Master' => array(
			'id' => '3162',
			'antibody_id' => null,
			'name' => 'Tagmentation Buffer (2x)',
			'description' => '<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>
</div>
<div class="small-12 medium-4 large-4 columns"><center>
<script>// <![CDATA[
 var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } 
// ]]></script>
</center></div>
</div>
<p></p>',
			'label1' => 'Examples of use',
			'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => '',
			'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label3' => '',
			'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'format' => '300 µl ',
			'catalog_number' => 'C01019043',
			'old_catalog_number' => '',
			'sf_code' => 'C01019043-29421',
			'type' => 'FRE',
			'search_order' => '',
			'price_EUR' => '90',
			'price_USD' => '100',
			'price_GBP' => '105',
			'price_JPY' => '14100',
			'price_CNY' => '',
			'price_AUD' => '250',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '',
			'slug' => 'tagmentation-buffer-2x',
			'meta_title' => 'Tagmentation Buffer 2x',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2023-06-20 18:19:03',
			'created' => '2020-12-08 16:00:14'
		),
		'Product' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		)
	),
	'Related' => array(),
	'Application' => array(),
	'Category' => array(
		(int) 0 => array(
			'id' => '141',
			'position' => '0',
			'parent_id' => '86',
			'name' => 'Tagmentation enzymes',
			'description' => '',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'tagmentation-enzymes',
			'cookies_tag_id' => null,
			'meta_keywords' => '',
			'meta_description' => '',
			'meta_title' => 'Tagmentation enzymes',
			'modified' => '2020-03-24 17:49:07',
			'created' => '2020-03-24 16:53:13',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '1115',
			'name' => 'Datasheet Tagmentation Buffer (2x) C01019043',
			'description' => '',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/kits/Datasheet_Tagmentation Buffer_2x_C01019043.pdf',
			'slug' => 'tagmentation-buffer-2x-datasheet',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2020-12-10 17:02:47',
			'created' => '2020-12-10 17:02:47',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '1776',
			'name' => 'product/kits/kit-icon.png',
			'alt' => 'Kit icon',
			'modified' => '2019-04-09 12:06:37',
			'created' => '2018-03-15 15:51:02',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '5002',
			'name' => 'HIRA protects telomeres against R-loop-induced instability in ALT cancer cells',
			'authors' => 'Michelle Lee Lynskey et al.',
			'description' => '<section id="author-highlights-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name">Highlights</h2>
<div id="abspara0020" role="paragraph">
<div id="ulist0010" role="list">
<div id="u0010" role="listitem">
<div class="content">
<div id="p0010" role="paragraph">HIRA establishes greater telomeric chromatin accessibility after ATRX-DAXX loss</div>
</div>
</div>
<div id="u0015" role="listitem">
<div class="content">
<div id="p0015" role="paragraph">Deposition of new H3.3 by HIRA-UBN restricts telomeric ssDNA and TERRA R-loops</div>
</div>
</div>
<div id="u0020" role="listitem">
<div class="content">
<div id="p0020" role="paragraph">Unresolved TERRA R-loops block new H3.3 deposition by HIRA-UBN</div>
</div>
</div>
<div id="u0025" role="listitem">
<div class="content">
<div id="p0025" role="paragraph">CHK1 phosphorylation of H3.3 is critical to prevent ssDNA and TERRA R-loop buildup</div>
</div>
</div>
</div>
</div>
</section>
<section id="author-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name">Summary</h2>
<div id="abspara0010" role="paragraph">Inactivating mutations in chromatin modifiers, like the &alpha;-thalassemia/mental retardation, X-linked (ATRX)-death domain-associated protein (DAXX) chromatin remodeling/histone H3.3 deposition complex, drive the cancer-specific alternative lengthening of telomeres (ALT) pathway. Prior studies revealed that HIRA, another histone H3.3 chaperone, compensates for ATRX-DAXX loss at telomeres to sustain ALT cancer cell survival. How HIRA rescues telomeres from the consequences of ATRX-DAXX deficiency remains unclear. Here, using an assay for transposase-accessible chromatin using sequencing (ATAC-seq) and cleavage under targets and release using nuclease (CUT&amp;RUN), we establish that HIRA-mediated deposition of new H3.3 maintains telomeric chromatin accessibility to prevent the detrimental accumulation of nucleosome-free single-stranded DNA (ssDNA) in ATRX-DAXX-deficient ALT cells. We show that the HIRA-UBN1/UBN2 complex deposits new H3.3 to prevent TERRA R-loop buildup and transcription-replication conflicts (TRCs) at telomeres. Furthermore, HIRA-mediated H3.3 incorporation into telomeric chromatin links productive ALT to the phosphorylation of serine 31, an H3.3-specific amino acid, by Chk1. Therefore, we identify a critical role for HIRA-mediated H3.3 deposition that ensures the survival of ATRX-DAXX-deficient ALT cancer cells.</div>
</section>
<section id="graphical-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name"></h2>
</section>',
			'date' => '2024-11-26',
			'pmid' => 'https://www.cell.com/cell-reports/fulltext/S2211-1247(24)01315-9',
			'doi' => '10.1016/j.celrep.2024.114964',
			'modified' => '2024-11-12 09:41:40',
			'created' => '2024-11-12 09:41:40',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '4985',
			'name' => 'HNF1β bookmarking involves Topoisomerase 1 activation and DNA topology relaxation in mitotic chromatin',
			'authors' => 'Alessia Bagattin et al.',
			'description' => '<section id="author-highlights-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name">Highlights</h2>
<div id="abspara0020" role="paragraph">
<div id="ulist0010" role="list">
<div id="u0010" role="listitem">
<div class="content">
<div id="p0010" role="paragraph">HNF1&beta; mitotic site binding is preserved with a specific methanol/formaldehyde ChIP</div>
</div>
</div>
<div id="u0015" role="listitem">
<div class="content">
<div id="p0015" role="paragraph">BTBD2, an HNF1&beta; partner, mediates mitosis-specific interaction with TOP1</div>
</div>
</div>
<div id="u0020" role="listitem">
<div class="content">
<div id="p0020" role="paragraph">HNF1&beta; recruits TOP1 and induces DNA relaxation around bookmarked HNF1&beta; sites</div>
</div>
</div>
<div id="u0025" role="listitem">
<div class="content">
<div id="p0025" role="paragraph">An HNF1&beta; mutation, found in MODY patients, disrupts the interaction with TOP1</div>
</div>
</div>
</div>
</div>
</section>
<section id="author-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name">Summary</h2>
<div id="abspara0010" role="paragraph">HNF1&beta; (<i>HNF1B</i>) is a transcription factor frequently mutated in patients with developmental renal disease. It binds to mitotic chromatin and reactivates gene expression after mitosis, a phenomenon referred to as bookmarking. Using a crosslinking method that circumvents the artifacts of formaldehyde, we demonstrate that&nbsp;HNF1&beta; remains associated with chromatin in a sequence-specific way in both interphase and mitosis. We identify an HNF1&beta;-interacting protein, BTBD2, that enables the interaction and activation of Topoisomerase 1 (TOP1) exclusively during mitosis. Our study identifies a shared microhomology domain between HNF1&beta; and TOP1, where a mutation, found in &ldquo;maturity onset diabetes of the young&rdquo; patients, disrupts their interaction. Importantly, HNF1&beta; recruits TOP1 and induces DNA relaxation around HNF1&beta; mitotic chromatin sites, elucidating its crucial role in chromatin remodeling and gene reactivation after mitotic exit. These findings shed light on how HNF1&beta; reactivates target gene expression after mitosis, providing insights into its crucial role in maintenance of cellular identity.</div>
</section>',
			'date' => '2024-10-08',
			'pmid' => 'https://www.cell.com/cell-reports/fulltext/S2211-1247(24)01156-2',
			'doi' => '10.1016/j.celrep.2024.114805',
			'modified' => '2024-10-14 09:04:44',
			'created' => '2024-10-14 09:04:44',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '4926',
			'name' => 'High-throughput sequencing of insect specimens with sub-optimal DNA preservation using a practical, plate-based Illumina-compatible Tn5 transposase library preparation method',
			'authors' => 'Cobb L. et all.',
			'description' => '<p><span>Entomological sampling and storage conditions often prioritise efficiency, practicality and conservation of morphological characteristics, and may therefore be suboptimal for DNA preservation. This practice can impact downstream molecular applications, such as the generation of high-throughput genomic libraries, which often requires substantial DNA input amounts. Here, we use a practical Tn5 transposase tagmentation-based library preparation method optimised for 96-well plates and low yield DNA extracts from insect legs that were stored under sub-optimal conditions for DNA preservation. The samples were kept in field vehicles for extended periods of time, before long-term storage in ethanol in the freezer, or dry at room temperature. By reducing DNA input to 6ng, more samples with sub-optimal DNA yields could be processed. We matched this low DNA input with a 6-fold dilution of a commercially available tagmentation enzyme, significantly reducing library preparation costs. Costs and workload were further suppressed by direct post-amplification pooling of individual libraries. We generated medium coverage (&gt;3-fold) genomes for 88 out of 90 specimens, with an average of approximately 10-fold coverage. While samples stored in ethanol yielded significantly less DNA compared to those which were stored dry, these samples had superior sequencing statistics, with longer sequencing reads and higher rates of endogenous DNA. Furthermore, we find that the efficiency of tagmentation-based library preparation can be improved by a thorough post-amplification bead clean-up which selects against both short and large DNA fragments. By opening opportunities for the use of sub-optimally preserved, low yield DNA extracts, we broaden the scope of whole genome studies of insect specimens. We therefore expect these results and this protocol to be valuable for a range of applications in the field of entomology.</span></p>',
			'date' => '2024-03-22',
			'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/38517905/',
			'doi' => '10.1371/journal.pone.0300865',
			'modified' => '2024-03-25 11:15:06',
			'created' => '2024-03-25 11:15:06',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '4923',
			'name' => 'On the identification of differentially-active transcription factors from ATAC-seq data',
			'authors' => 'Gerbaldo F. et al.',
			'description' => '<p><span>ATAC-seq has emerged as a rich epigenome profiling technique, and is commonly used to identify Transcription Factors (TFs) underlying given phenomena. A number of methods can be used to identify differentially-active TFs through the accessibility of their DNA-binding motif, however little is known on the best approaches for doing so. Here we benchmark several such methods using a combination of curated datasets with various forms of short-term perturbations on known TFs, as well as semi-simulations. We include both methods specifically designed for this type of data as well as some that can be repurposed for it. We also investigate variations to these methods, and identify three particularly promising approaches (chromVAR-limma with critical adjustments, monaLisa and a combination of GC smooth quantile normalization and multivariate modeling). We further investigate the specific use of nucleosome-free fragments, the combination of top methods, and the impact of technical variation. Finally, we illustrate the use of the top methods on a novel dataset to characterize the impact on DNA accessibility of TRAnscription Factor TArgeting Chimeras (TRAFTAC), which can deplete TFs &ndash; in our case NFkB &ndash; at the protein level.</span></p>',
			'date' => '2024-03-10',
			'pmid' => 'https://www.biorxiv.org/content/10.1101/2024.03.06.583825v2',
			'doi' => 'https://doi.org/10.1101/2024.03.06.583825',
			'modified' => '2024-03-13 17:04:33',
			'created' => '2024-03-13 17:04:33',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '4878',
			'name' => 'ARID1A governs the silencing of sex-linked transcription during male meiosis in the mouse',
			'authors' => 'Menon D.U. et al.',
			'description' => '<p><span>We present evidence implicating the BAF (BRG1/BRM Associated Factor) chromatin remodeler in meiotic sex chromosome inactivation (MSCI). By immunofluorescence (IF), the putative BAF DNA binding subunit, ARID1A (AT-rich Interaction Domain 1a), appeared enriched on the male sex chromosomes during diplonema of meiosis I. The germ cell-specific depletion of ARID1A resulted in a pachynema arrest and failure to repress sex-linked genes, indicating a defective MSCI. Consistent with this defect, mutant sex chromosomes displayed an abnormal presence of elongating RNA polymerase II coupled with an overall increase in chromatin accessibility detectable by ATAC-seq. By investigating potential mechanisms underlying these anomalies, we identified a role for ARID1A in promoting the preferential enrichment of the histone variant, H3.3, on the sex chromosomes, a known hallmark of MSCI. Without ARID1A, the sex chromosomes appeared depleted of H3.3 at levels resembling autosomes. Higher resolution analyses by CUT&amp;RUN revealed shifts in sex-linked H3.3 associations from discrete intergenic sites and broader gene-body domains to promoters in response to the loss of ARID1A. Several sex-linked sites displayed ectopic H3.3 occupancy that did not co-localize with DMC1 (DNA Meiotic Recombinase 1). This observation suggests a requirement for ARID1A in DMC1 localization to the asynapsed sex chromatids. We conclude that ARID1A-directed H3.3 localization influences meiotic sex chromosome gene regulation and DNA repair.</span></p>',
			'date' => '2023-09-28',
			'pmid' => 'https://www.biorxiv.org/content/10.1101/2023.05.25.542290v2.abstract',
			'doi' => 'https://doi.org/10.1101/2023.05.25.542290',
			'modified' => '2023-11-10 14:53:09',
			'created' => '2023-11-10 14:53:09',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '4817',
			'name' => 'YAP/BRD4-controlled ROR1 promotes tumor-initiating cells andhyperproliferation in pancreatic cancer.',
			'authors' => 'Yamazaki  M. et al.',
			'description' => '<p><span>Tumor-initiating cells are major drivers of chemoresistance and attractive targets for cancer therapy, however, their identity in human pancreatic ductal adenocarcinoma (PDAC) and the key molecules underlying their traits remain poorly understood. Here, we show that a cellular subpopulation with partial epithelial-mesenchymal transition (EMT)-like signature marked by high expression of receptor tyrosine kinase-like orphan receptor 1 (ROR1) is the origin of heterogeneous tumor cells in PDAC. We demonstrate that ROR1 depletion suppresses tumor growth, recurrence after chemotherapy, and metastasis. Mechanistically, ROR1 induces the expression of Aurora kinase B (AURKB) by activating E2F through c-Myc to enhance PDAC proliferation. Furthermore, epigenomic analyses reveal that ROR1 is transcriptionally dependent on YAP/BRD4 binding at the enhancer region, and targeting this pathway reduces ROR1 expression and prevents PDAC growth. Collectively, our findings reveal a critical role for ROR1high cells as tumor-initiating cells and the functional importance of ROR1 in PDAC progression, thereby highlighting its therapeutic targetability.</span></p>',
			'date' => '2023-04-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37096681',
			'doi' => '10.15252/embj.2022112614',
			'modified' => '2023-06-15 10:06:12',
			'created' => '2023-06-13 21:11:31',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '4742',
			'name' => 'A neurodevelopmental epigenetic programme mediated bySMARCD3-DAB1-Reelin signalling is hijacked to promote medulloblastomametastasis.',
			'authors' => 'Zou  Han et al.',
			'description' => '<p>How abnormal neurodevelopment relates to the tumour aggressiveness of medulloblastoma (MB), the most common type of embryonal tumour, remains elusive. Here we uncover a neurodevelopmental epigenomic programme that is hijacked to induce MB metastatic dissemination. Unsupervised analyses of integrated publicly available datasets with our newly generated data reveal that SMARCD3 (also known as BAF60C) regulates Disabled&acirc;&euro;&permil;1 (DAB1)-mediated Reelin signalling in Purkinje cell migration and MB metastasis by orchestrating cis-regulatory elements at the DAB1 locus. We further identify that a core set of transcription factors, enhancer of zeste homologue&acirc;&euro;&permil;2 (EZH2) and nuclear factor&acirc;&euro;&permil;I&acirc;&euro;&permil;X (NFIX), coordinates with the cis-regulatory elements at the SMARCD3 locus to form a chromatin hub to control SMARCD3 expression in the developing cerebellum and in metastatic MB. Increased SMARCD3 expression activates Reelin-DAB1-mediated Src kinase signalling, which results in a MB response to Src inhibition. These data deepen our understanding of how neurodevelopmental programming influences disease progression and provide a potential therapeutic option for patients with MB.</p>',
			'date' => '2023-02-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36849558',
			'doi' => '10.1038/s41556-023-01093-0',
			'modified' => '2023-03-14 09:41:24',
			'created' => '2023-03-02 17:27:08',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '4660',
			'name' => 'EBF1 is continuously required for stabilizing local chromatinaccessibility in pro-B cells.',
			'authors' => 'Zolotarev  Nikolay et al.',
			'description' => '<p>The establishment of de novo chromatin accessibility in lymphoid progenitors requires the "pioneering" function of transcription factor (TF) early B cell factor 1 (EBF1), which binds to na&iuml;ve chromatin and induces accessibility by recruiting the BRG1 chromatin remodeler subunit. However, it remains unclear whether the function of EBF1 is continuously required for stabilizing local chromatin accessibility. To this end, we replaced EBF1 by EBF1-FKBP in pro-B cells, allowing the rapid degradation by adding the degradation TAG13 (dTAG13) dimerizer. EBF1 degradation results in a loss of genome-wide EBF1 occupancy and EBF1-targeted BRG1 binding. Chromatin accessibility was rapidly diminished at EBF1-binding sites with a preference for sites whose occupancy requires the pioneering activity of the C-terminal domain of EBF1. Diminished chromatin accessibility correlated with altered gene expression. Thus, continuous activity of EBF1 is required for the stable maintenance of the transcriptional and epigenetic state of pro-B cells.</p>',
			'date' => '2022-11-01',
			'pmid' => 'https://doi.org/10.1073%2Fpnas',
			'doi' => '10.1073/pnas.2210595119',
			'modified' => '2023-03-07 09:07:41',
			'created' => '2023-02-21 09:59:46',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '2118',
			'name' => 'Tagmentation Buffer (2x) SDS GB en',
			'language' => 'en',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-GB-en-GHS_1_0.pdf',
			'countries' => 'GB',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '2120',
			'name' => 'Tagmentation Buffer (2x) SDS US en',
			'language' => 'en',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-US-en-GHS_1_0.pdf',
			'countries' => 'US',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '2116',
			'name' => 'Tagmentation Buffer (2x) SDS ES es',
			'language' => 'es',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-ES-es-GHS_1_0.pdf',
			'countries' => 'ES',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '2119',
			'name' => 'Tagmentation Buffer (2x) SDS JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-JP-ja-GHS_2_0.pdf',
			'countries' => 'JP',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '2115',
			'name' => 'Tagmentation Buffer (2x) SDS DE de',
			'language' => 'de',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-DE-de-GHS_1_0.pdf',
			'countries' => 'DE',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '2113',
			'name' => 'Tagmentation Buffer (2x) SDS BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-BE-fr-GHS_1_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '2114',
			'name' => 'Tagmentation Buffer (2x) SDS BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-BE-nl-GHS_1_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '2117',
			'name' => 'Tagmentation Buffer (2x) SDS FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-FR-fr-GHS_1_0.pdf',
			'countries' => 'FR',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/tagmentation-buffer-2x'
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
	(int) 0 => array(
		'id' => '3162',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>
</div>
<div class="small-12 medium-4 large-4 columns"><center>
<script>// <![CDATA[
 var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } 
// ]]></script>
</center></div>
</div>
<p></p>',
		'label1' => 'Examples of use',
		'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label3' => '',
		'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'format' => '300 µl ',
		'catalog_number' => 'C01019043',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-29421',
		'type' => 'FRE',
		'search_order' => '',
		'price_EUR' => '90',
		'price_USD' => '100',
		'price_GBP' => '105',
		'price_JPY' => '14100',
		'price_CNY' => '',
		'price_AUD' => '250',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x',
		'meta_title' => 'Tagmentation Buffer 2x',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2023-06-20 18:19:03',
		'created' => '2020-12-08 16:00:14'
	),
	(int) 1 => array(
		'id' => '3163',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
		'label1' => 'Examples of use',
		'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label3' => '',
		'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'format' => '100 μl',
		'catalog_number' => 'C01019043-100',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-D001-001161',
		'type' => 'FRE',
		'search_order' => '',
		'price_EUR' => '50',
		'price_USD' => '50',
		'price_GBP' => '55',
		'price_JPY' => '7830',
		'price_CNY' => '',
		'price_AUD' => '125',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x-100',
		'meta_title' => 'Tagmentation Buffer 2x 100',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2022-11-25 12:25:10',
		'created' => '2020-12-08 16:06:10',
		'ProductsGroup' => array(
			'id' => '371',
			'product_id' => '3163',
			'group_id' => '330'
		)
	),
	(int) 2 => array(
		'id' => '3217',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
		'label1' => 'Examples of use',
		'info1' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label3' => '',
		'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'format' => '1000 µl',
		'catalog_number' => 'C01019043-1000',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-178850',
		'type' => 'FRE',
		'search_order' => '',
		'price_EUR' => '225',
		'price_USD' => '255',
		'price_GBP' => '270',
		'price_JPY' => '35245',
		'price_CNY' => '',
		'price_AUD' => '638',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x-1000',
		'meta_title' => 'Tagmentation Buffer 2x',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2023-01-04 15:29:39',
		'created' => '2022-04-13 15:31:11',
		'ProductsGroup' => array(
			'id' => '405',
			'product_id' => '3217',
			'group_id' => '330'
		)
	),
	(int) 3 => array(
		'id' => '3229',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
		'label1' => 'Examples of use',
		'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '10000 µl',
		'catalog_number' => 'C01019043-10000',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-190331',
		'type' => 'FRE',
		'search_order' => '1400, GBP 1640, USD 1500, AUD 3750, JPY ¥244,000',
		'price_EUR' => '1475',
		'price_USD' => '1585',
		'price_GBP' => '1730',
		'price_JPY' => '231060',
		'price_CNY' => '/',
		'price_AUD' => '3962',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x-10000',
		'meta_title' => 'Tagmentation Buffer 2x',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2022-11-25 12:29:09',
		'created' => '2022-11-25 11:26:45',
		'ProductsGroup' => array(
			'id' => '412',
			'product_id' => '3229',
			'group_id' => '330'
		)
	)
)
$pro = array(
	'id' => '3229',
	'antibody_id' => null,
	'name' => 'Tagmentation Buffer (2x)',
	'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
	'label1' => 'Examples of use',
	'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '10000 µl',
	'catalog_number' => 'C01019043-10000',
	'old_catalog_number' => '',
	'sf_code' => 'C01019043-190331',
	'type' => 'FRE',
	'search_order' => '1400, GBP 1640, USD 1500, AUD 3750, JPY ¥244,000',
	'price_EUR' => '1475',
	'price_USD' => '1585',
	'price_GBP' => '1730',
	'price_JPY' => '231060',
	'price_CNY' => '/',
	'price_AUD' => '3962',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '',
	'slug' => 'tagmentation-buffer-2x-10000',
	'meta_title' => 'Tagmentation Buffer 2x',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2022-11-25 12:29:09',
	'created' => '2022-11-25 11:26:45',
	'ProductsGroup' => array(
		'id' => '412',
		'product_id' => '3229',
		'group_id' => '330'
	)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
	'id' => '3229',
	'antibody_id' => null,
	'name' => 'Tagmentation Buffer (2x)',
	'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
	'label1' => 'Examples of use',
	'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '10000 µl',
	'catalog_number' => 'C01019043-10000',
	'old_catalog_number' => '',
	'sf_code' => 'C01019043-190331',
	'type' => 'FRE',
	'search_order' => '1400, GBP 1640, USD 1500, AUD 3750, JPY ¥244,000',
	'price_EUR' => '1475',
	'price_USD' => '1585',
	'price_GBP' => '1730',
	'price_JPY' => '231060',
	'price_CNY' => '/',
	'price_AUD' => '3962',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '',
	'slug' => 'tagmentation-buffer-2x-10000',
	'meta_title' => 'Tagmentation Buffer 2x',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2022-11-25 12:29:09',
	'created' => '2022-11-25 11:26:45',
	'ProductsGroup' => array(
		'id' => '412',
		'product_id' => '3229',
		'group_id' => '330'
	)
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '1115',
	'name' => 'Datasheet Tagmentation Buffer (2x) C01019043',
	'description' => '',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/kits/Datasheet_Tagmentation Buffer_2x_C01019043.pdf',
	'slug' => 'tagmentation-buffer-2x-datasheet',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2020-12-10 17:02:47',
	'created' => '2020-12-10 17:02:47',
	'ProductsDocument' => array(
		'id' => '3259',
		'product_id' => '3228',
		'document_id' => '1115'
	)
)
$sds = array(
	'id' => '2117',
	'name' => 'Tagmentation Buffer (2x) SDS FR fr',
	'language' => 'fr',
	'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-FR-fr-GHS_1_0.pdf',
	'countries' => 'FR',
	'modified' => '2021-10-12 14:27:13',
	'created' => '2021-10-12 14:27:13',
	'ProductsSafetySheet' => array(
		'id' => '4772',
		'product_id' => '3228',
		'safety_sheet_id' => '2117'
	)
)
$publication = array(
	'id' => '4660',
	'name' => 'EBF1 is continuously required for stabilizing local chromatinaccessibility in pro-B cells.',
	'authors' => 'Zolotarev  Nikolay et al.',
	'description' => '<p>The establishment of de novo chromatin accessibility in lymphoid progenitors requires the "pioneering" function of transcription factor (TF) early B cell factor 1 (EBF1), which binds to na&iuml;ve chromatin and induces accessibility by recruiting the BRG1 chromatin remodeler subunit. However, it remains unclear whether the function of EBF1 is continuously required for stabilizing local chromatin accessibility. To this end, we replaced EBF1 by EBF1-FKBP in pro-B cells, allowing the rapid degradation by adding the degradation TAG13 (dTAG13) dimerizer. EBF1 degradation results in a loss of genome-wide EBF1 occupancy and EBF1-targeted BRG1 binding. Chromatin accessibility was rapidly diminished at EBF1-binding sites with a preference for sites whose occupancy requires the pioneering activity of the C-terminal domain of EBF1. Diminished chromatin accessibility correlated with altered gene expression. Thus, continuous activity of EBF1 is required for the stable maintenance of the transcriptional and epigenetic state of pro-B cells.</p>',
	'date' => '2022-11-01',
	'pmid' => 'https://doi.org/10.1073%2Fpnas',
	'doi' => '10.1073/pnas.2210595119',
	'modified' => '2023-03-07 09:07:41',
	'created' => '2023-02-21 09:59:46',
	'ProductsPublication' => array(
		'id' => '6556',
		'product_id' => '3228',
		'publication_id' => '4660'
	)
)
$externalLink = ' <a href="https://doi.org/10.1073%2Fpnas" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'cn',
	'meta_keywords' => '',
	'meta_description' => '',
	'meta_title' => 'Tagmentation Buffer 2x',
	'product' => array(
		'Product' => array(
			'id' => '3228',
			'antibody_id' => null,
			'name' => 'Tagmentation Buffer (2x)',
			'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
			'label1' => 'Examples of use',
			'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '5000 µl',
			'catalog_number' => 'C01019043-5000',
			'old_catalog_number' => '',
			'sf_code' => 'C01019043-190330',
			'type' => 'FRE',
			'search_order' => '',
			'price_EUR' => '845',
			'price_USD' => '940',
			'price_GBP' => '990',
			'price_JPY' => '132370',
			'price_CNY' => '',
			'price_AUD' => '2350',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => false,
			'last_datasheet_update' => '',
			'slug' => 'tagmentation-buffer-2x-5000',
			'meta_title' => 'Tagmentation Buffer 2x',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-11-25 12:27:51',
			'created' => '2022-11-25 11:18:25',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(),
		'Group' => array(
			'Group' => array(
				[maximum depth reached]
			),
			'Master' => array(
				[maximum depth reached]
			),
			'Product' => array(
				[maximum depth reached]
			)
		),
		'Related' => array(),
		'Application' => array(),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	),
	'meta_canonical' => 'https://www.diagenode.com/cn/p/tagmentation-buffer-2x'
)
$language = 'cn'
$meta_keywords = ''
$meta_description = ''
$meta_title = 'Tagmentation Buffer 2x'
$product = array(
	'Product' => array(
		'id' => '3228',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
		'label1' => 'Examples of use',
		'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label3' => '',
		'info3' => '',
		'format' => '5000 µl',
		'catalog_number' => 'C01019043-5000',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-190330',
		'type' => 'FRE',
		'search_order' => '',
		'price_EUR' => '845',
		'price_USD' => '940',
		'price_GBP' => '990',
		'price_JPY' => '132370',
		'price_CNY' => '',
		'price_AUD' => '2350',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x-5000',
		'meta_title' => 'Tagmentation Buffer 2x',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2022-11-25 12:27:51',
		'created' => '2022-11-25 11:18:25',
		'locale' => 'zho'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(),
	'Group' => array(
		'Group' => array(
			'id' => '330',
			'name' => 'C01019043',
			'product_id' => '3162',
			'modified' => '2022-11-25 11:27:26',
			'created' => '2020-12-08 16:06:34'
		),
		'Master' => array(
			'id' => '3162',
			'antibody_id' => null,
			'name' => 'Tagmentation Buffer (2x)',
			'description' => '<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>
</div>
<div class="small-12 medium-4 large-4 columns"><center>
<script>// <![CDATA[
 var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } 
// ]]></script>
</center></div>
</div>
<p></p>',
			'label1' => 'Examples of use',
			'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => '',
			'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label3' => '',
			'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'format' => '300 µl ',
			'catalog_number' => 'C01019043',
			'old_catalog_number' => '',
			'sf_code' => 'C01019043-29421',
			'type' => 'FRE',
			'search_order' => '',
			'price_EUR' => '90',
			'price_USD' => '100',
			'price_GBP' => '105',
			'price_JPY' => '14100',
			'price_CNY' => '',
			'price_AUD' => '250',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '',
			'slug' => 'tagmentation-buffer-2x',
			'meta_title' => 'Tagmentation Buffer 2x',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2023-06-20 18:19:03',
			'created' => '2020-12-08 16:00:14'
		),
		'Product' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		)
	),
	'Related' => array(),
	'Application' => array(),
	'Category' => array(
		(int) 0 => array(
			'id' => '141',
			'position' => '0',
			'parent_id' => '86',
			'name' => 'Tagmentation enzymes',
			'description' => '',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'tagmentation-enzymes',
			'cookies_tag_id' => null,
			'meta_keywords' => '',
			'meta_description' => '',
			'meta_title' => 'Tagmentation enzymes',
			'modified' => '2020-03-24 17:49:07',
			'created' => '2020-03-24 16:53:13',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '1115',
			'name' => 'Datasheet Tagmentation Buffer (2x) C01019043',
			'description' => '',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/kits/Datasheet_Tagmentation Buffer_2x_C01019043.pdf',
			'slug' => 'tagmentation-buffer-2x-datasheet',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2020-12-10 17:02:47',
			'created' => '2020-12-10 17:02:47',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '1776',
			'name' => 'product/kits/kit-icon.png',
			'alt' => 'Kit icon',
			'modified' => '2019-04-09 12:06:37',
			'created' => '2018-03-15 15:51:02',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '5002',
			'name' => 'HIRA protects telomeres against R-loop-induced instability in ALT cancer cells',
			'authors' => 'Michelle Lee Lynskey et al.',
			'description' => '<section id="author-highlights-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name">Highlights</h2>
<div id="abspara0020" role="paragraph">
<div id="ulist0010" role="list">
<div id="u0010" role="listitem">
<div class="content">
<div id="p0010" role="paragraph">HIRA establishes greater telomeric chromatin accessibility after ATRX-DAXX loss</div>
</div>
</div>
<div id="u0015" role="listitem">
<div class="content">
<div id="p0015" role="paragraph">Deposition of new H3.3 by HIRA-UBN restricts telomeric ssDNA and TERRA R-loops</div>
</div>
</div>
<div id="u0020" role="listitem">
<div class="content">
<div id="p0020" role="paragraph">Unresolved TERRA R-loops block new H3.3 deposition by HIRA-UBN</div>
</div>
</div>
<div id="u0025" role="listitem">
<div class="content">
<div id="p0025" role="paragraph">CHK1 phosphorylation of H3.3 is critical to prevent ssDNA and TERRA R-loop buildup</div>
</div>
</div>
</div>
</div>
</section>
<section id="author-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name">Summary</h2>
<div id="abspara0010" role="paragraph">Inactivating mutations in chromatin modifiers, like the &alpha;-thalassemia/mental retardation, X-linked (ATRX)-death domain-associated protein (DAXX) chromatin remodeling/histone H3.3 deposition complex, drive the cancer-specific alternative lengthening of telomeres (ALT) pathway. Prior studies revealed that HIRA, another histone H3.3 chaperone, compensates for ATRX-DAXX loss at telomeres to sustain ALT cancer cell survival. How HIRA rescues telomeres from the consequences of ATRX-DAXX deficiency remains unclear. Here, using an assay for transposase-accessible chromatin using sequencing (ATAC-seq) and cleavage under targets and release using nuclease (CUT&amp;RUN), we establish that HIRA-mediated deposition of new H3.3 maintains telomeric chromatin accessibility to prevent the detrimental accumulation of nucleosome-free single-stranded DNA (ssDNA) in ATRX-DAXX-deficient ALT cells. We show that the HIRA-UBN1/UBN2 complex deposits new H3.3 to prevent TERRA R-loop buildup and transcription-replication conflicts (TRCs) at telomeres. Furthermore, HIRA-mediated H3.3 incorporation into telomeric chromatin links productive ALT to the phosphorylation of serine 31, an H3.3-specific amino acid, by Chk1. Therefore, we identify a critical role for HIRA-mediated H3.3 deposition that ensures the survival of ATRX-DAXX-deficient ALT cancer cells.</div>
</section>
<section id="graphical-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name"></h2>
</section>',
			'date' => '2024-11-26',
			'pmid' => 'https://www.cell.com/cell-reports/fulltext/S2211-1247(24)01315-9',
			'doi' => '10.1016/j.celrep.2024.114964',
			'modified' => '2024-11-12 09:41:40',
			'created' => '2024-11-12 09:41:40',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '4985',
			'name' => 'HNF1β bookmarking involves Topoisomerase 1 activation and DNA topology relaxation in mitotic chromatin',
			'authors' => 'Alessia Bagattin et al.',
			'description' => '<section id="author-highlights-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name">Highlights</h2>
<div id="abspara0020" role="paragraph">
<div id="ulist0010" role="list">
<div id="u0010" role="listitem">
<div class="content">
<div id="p0010" role="paragraph">HNF1&beta; mitotic site binding is preserved with a specific methanol/formaldehyde ChIP</div>
</div>
</div>
<div id="u0015" role="listitem">
<div class="content">
<div id="p0015" role="paragraph">BTBD2, an HNF1&beta; partner, mediates mitosis-specific interaction with TOP1</div>
</div>
</div>
<div id="u0020" role="listitem">
<div class="content">
<div id="p0020" role="paragraph">HNF1&beta; recruits TOP1 and induces DNA relaxation around bookmarked HNF1&beta; sites</div>
</div>
</div>
<div id="u0025" role="listitem">
<div class="content">
<div id="p0025" role="paragraph">An HNF1&beta; mutation, found in MODY patients, disrupts the interaction with TOP1</div>
</div>
</div>
</div>
</div>
</section>
<section id="author-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name">Summary</h2>
<div id="abspara0010" role="paragraph">HNF1&beta; (<i>HNF1B</i>) is a transcription factor frequently mutated in patients with developmental renal disease. It binds to mitotic chromatin and reactivates gene expression after mitosis, a phenomenon referred to as bookmarking. Using a crosslinking method that circumvents the artifacts of formaldehyde, we demonstrate that&nbsp;HNF1&beta; remains associated with chromatin in a sequence-specific way in both interphase and mitosis. We identify an HNF1&beta;-interacting protein, BTBD2, that enables the interaction and activation of Topoisomerase 1 (TOP1) exclusively during mitosis. Our study identifies a shared microhomology domain between HNF1&beta; and TOP1, where a mutation, found in &ldquo;maturity onset diabetes of the young&rdquo; patients, disrupts their interaction. Importantly, HNF1&beta; recruits TOP1 and induces DNA relaxation around HNF1&beta; mitotic chromatin sites, elucidating its crucial role in chromatin remodeling and gene reactivation after mitotic exit. These findings shed light on how HNF1&beta; reactivates target gene expression after mitosis, providing insights into its crucial role in maintenance of cellular identity.</div>
</section>',
			'date' => '2024-10-08',
			'pmid' => 'https://www.cell.com/cell-reports/fulltext/S2211-1247(24)01156-2',
			'doi' => '10.1016/j.celrep.2024.114805',
			'modified' => '2024-10-14 09:04:44',
			'created' => '2024-10-14 09:04:44',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '4926',
			'name' => 'High-throughput sequencing of insect specimens with sub-optimal DNA preservation using a practical, plate-based Illumina-compatible Tn5 transposase library preparation method',
			'authors' => 'Cobb L. et all.',
			'description' => '<p><span>Entomological sampling and storage conditions often prioritise efficiency, practicality and conservation of morphological characteristics, and may therefore be suboptimal for DNA preservation. This practice can impact downstream molecular applications, such as the generation of high-throughput genomic libraries, which often requires substantial DNA input amounts. Here, we use a practical Tn5 transposase tagmentation-based library preparation method optimised for 96-well plates and low yield DNA extracts from insect legs that were stored under sub-optimal conditions for DNA preservation. The samples were kept in field vehicles for extended periods of time, before long-term storage in ethanol in the freezer, or dry at room temperature. By reducing DNA input to 6ng, more samples with sub-optimal DNA yields could be processed. We matched this low DNA input with a 6-fold dilution of a commercially available tagmentation enzyme, significantly reducing library preparation costs. Costs and workload were further suppressed by direct post-amplification pooling of individual libraries. We generated medium coverage (&gt;3-fold) genomes for 88 out of 90 specimens, with an average of approximately 10-fold coverage. While samples stored in ethanol yielded significantly less DNA compared to those which were stored dry, these samples had superior sequencing statistics, with longer sequencing reads and higher rates of endogenous DNA. Furthermore, we find that the efficiency of tagmentation-based library preparation can be improved by a thorough post-amplification bead clean-up which selects against both short and large DNA fragments. By opening opportunities for the use of sub-optimally preserved, low yield DNA extracts, we broaden the scope of whole genome studies of insect specimens. We therefore expect these results and this protocol to be valuable for a range of applications in the field of entomology.</span></p>',
			'date' => '2024-03-22',
			'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/38517905/',
			'doi' => '10.1371/journal.pone.0300865',
			'modified' => '2024-03-25 11:15:06',
			'created' => '2024-03-25 11:15:06',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '4923',
			'name' => 'On the identification of differentially-active transcription factors from ATAC-seq data',
			'authors' => 'Gerbaldo F. et al.',
			'description' => '<p><span>ATAC-seq has emerged as a rich epigenome profiling technique, and is commonly used to identify Transcription Factors (TFs) underlying given phenomena. A number of methods can be used to identify differentially-active TFs through the accessibility of their DNA-binding motif, however little is known on the best approaches for doing so. Here we benchmark several such methods using a combination of curated datasets with various forms of short-term perturbations on known TFs, as well as semi-simulations. We include both methods specifically designed for this type of data as well as some that can be repurposed for it. We also investigate variations to these methods, and identify three particularly promising approaches (chromVAR-limma with critical adjustments, monaLisa and a combination of GC smooth quantile normalization and multivariate modeling). We further investigate the specific use of nucleosome-free fragments, the combination of top methods, and the impact of technical variation. Finally, we illustrate the use of the top methods on a novel dataset to characterize the impact on DNA accessibility of TRAnscription Factor TArgeting Chimeras (TRAFTAC), which can deplete TFs &ndash; in our case NFkB &ndash; at the protein level.</span></p>',
			'date' => '2024-03-10',
			'pmid' => 'https://www.biorxiv.org/content/10.1101/2024.03.06.583825v2',
			'doi' => 'https://doi.org/10.1101/2024.03.06.583825',
			'modified' => '2024-03-13 17:04:33',
			'created' => '2024-03-13 17:04:33',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '4878',
			'name' => 'ARID1A governs the silencing of sex-linked transcription during male meiosis in the mouse',
			'authors' => 'Menon D.U. et al.',
			'description' => '<p><span>We present evidence implicating the BAF (BRG1/BRM Associated Factor) chromatin remodeler in meiotic sex chromosome inactivation (MSCI). By immunofluorescence (IF), the putative BAF DNA binding subunit, ARID1A (AT-rich Interaction Domain 1a), appeared enriched on the male sex chromosomes during diplonema of meiosis I. The germ cell-specific depletion of ARID1A resulted in a pachynema arrest and failure to repress sex-linked genes, indicating a defective MSCI. Consistent with this defect, mutant sex chromosomes displayed an abnormal presence of elongating RNA polymerase II coupled with an overall increase in chromatin accessibility detectable by ATAC-seq. By investigating potential mechanisms underlying these anomalies, we identified a role for ARID1A in promoting the preferential enrichment of the histone variant, H3.3, on the sex chromosomes, a known hallmark of MSCI. Without ARID1A, the sex chromosomes appeared depleted of H3.3 at levels resembling autosomes. Higher resolution analyses by CUT&amp;RUN revealed shifts in sex-linked H3.3 associations from discrete intergenic sites and broader gene-body domains to promoters in response to the loss of ARID1A. Several sex-linked sites displayed ectopic H3.3 occupancy that did not co-localize with DMC1 (DNA Meiotic Recombinase 1). This observation suggests a requirement for ARID1A in DMC1 localization to the asynapsed sex chromatids. We conclude that ARID1A-directed H3.3 localization influences meiotic sex chromosome gene regulation and DNA repair.</span></p>',
			'date' => '2023-09-28',
			'pmid' => 'https://www.biorxiv.org/content/10.1101/2023.05.25.542290v2.abstract',
			'doi' => 'https://doi.org/10.1101/2023.05.25.542290',
			'modified' => '2023-11-10 14:53:09',
			'created' => '2023-11-10 14:53:09',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '4817',
			'name' => 'YAP/BRD4-controlled ROR1 promotes tumor-initiating cells andhyperproliferation in pancreatic cancer.',
			'authors' => 'Yamazaki  M. et al.',
			'description' => '<p><span>Tumor-initiating cells are major drivers of chemoresistance and attractive targets for cancer therapy, however, their identity in human pancreatic ductal adenocarcinoma (PDAC) and the key molecules underlying their traits remain poorly understood. Here, we show that a cellular subpopulation with partial epithelial-mesenchymal transition (EMT)-like signature marked by high expression of receptor tyrosine kinase-like orphan receptor 1 (ROR1) is the origin of heterogeneous tumor cells in PDAC. We demonstrate that ROR1 depletion suppresses tumor growth, recurrence after chemotherapy, and metastasis. Mechanistically, ROR1 induces the expression of Aurora kinase B (AURKB) by activating E2F through c-Myc to enhance PDAC proliferation. Furthermore, epigenomic analyses reveal that ROR1 is transcriptionally dependent on YAP/BRD4 binding at the enhancer region, and targeting this pathway reduces ROR1 expression and prevents PDAC growth. Collectively, our findings reveal a critical role for ROR1high cells as tumor-initiating cells and the functional importance of ROR1 in PDAC progression, thereby highlighting its therapeutic targetability.</span></p>',
			'date' => '2023-04-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37096681',
			'doi' => '10.15252/embj.2022112614',
			'modified' => '2023-06-15 10:06:12',
			'created' => '2023-06-13 21:11:31',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '4742',
			'name' => 'A neurodevelopmental epigenetic programme mediated bySMARCD3-DAB1-Reelin signalling is hijacked to promote medulloblastomametastasis.',
			'authors' => 'Zou  Han et al.',
			'description' => '<p>How abnormal neurodevelopment relates to the tumour aggressiveness of medulloblastoma (MB), the most common type of embryonal tumour, remains elusive. Here we uncover a neurodevelopmental epigenomic programme that is hijacked to induce MB metastatic dissemination. Unsupervised analyses of integrated publicly available datasets with our newly generated data reveal that SMARCD3 (also known as BAF60C) regulates Disabled&acirc;&euro;&permil;1 (DAB1)-mediated Reelin signalling in Purkinje cell migration and MB metastasis by orchestrating cis-regulatory elements at the DAB1 locus. We further identify that a core set of transcription factors, enhancer of zeste homologue&acirc;&euro;&permil;2 (EZH2) and nuclear factor&acirc;&euro;&permil;I&acirc;&euro;&permil;X (NFIX), coordinates with the cis-regulatory elements at the SMARCD3 locus to form a chromatin hub to control SMARCD3 expression in the developing cerebellum and in metastatic MB. Increased SMARCD3 expression activates Reelin-DAB1-mediated Src kinase signalling, which results in a MB response to Src inhibition. These data deepen our understanding of how neurodevelopmental programming influences disease progression and provide a potential therapeutic option for patients with MB.</p>',
			'date' => '2023-02-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36849558',
			'doi' => '10.1038/s41556-023-01093-0',
			'modified' => '2023-03-14 09:41:24',
			'created' => '2023-03-02 17:27:08',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '4660',
			'name' => 'EBF1 is continuously required for stabilizing local chromatinaccessibility in pro-B cells.',
			'authors' => 'Zolotarev  Nikolay et al.',
			'description' => '<p>The establishment of de novo chromatin accessibility in lymphoid progenitors requires the "pioneering" function of transcription factor (TF) early B cell factor 1 (EBF1), which binds to na&iuml;ve chromatin and induces accessibility by recruiting the BRG1 chromatin remodeler subunit. However, it remains unclear whether the function of EBF1 is continuously required for stabilizing local chromatin accessibility. To this end, we replaced EBF1 by EBF1-FKBP in pro-B cells, allowing the rapid degradation by adding the degradation TAG13 (dTAG13) dimerizer. EBF1 degradation results in a loss of genome-wide EBF1 occupancy and EBF1-targeted BRG1 binding. Chromatin accessibility was rapidly diminished at EBF1-binding sites with a preference for sites whose occupancy requires the pioneering activity of the C-terminal domain of EBF1. Diminished chromatin accessibility correlated with altered gene expression. Thus, continuous activity of EBF1 is required for the stable maintenance of the transcriptional and epigenetic state of pro-B cells.</p>',
			'date' => '2022-11-01',
			'pmid' => 'https://doi.org/10.1073%2Fpnas',
			'doi' => '10.1073/pnas.2210595119',
			'modified' => '2023-03-07 09:07:41',
			'created' => '2023-02-21 09:59:46',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '2118',
			'name' => 'Tagmentation Buffer (2x) SDS GB en',
			'language' => 'en',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-GB-en-GHS_1_0.pdf',
			'countries' => 'GB',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '2120',
			'name' => 'Tagmentation Buffer (2x) SDS US en',
			'language' => 'en',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-US-en-GHS_1_0.pdf',
			'countries' => 'US',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '2116',
			'name' => 'Tagmentation Buffer (2x) SDS ES es',
			'language' => 'es',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-ES-es-GHS_1_0.pdf',
			'countries' => 'ES',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '2119',
			'name' => 'Tagmentation Buffer (2x) SDS JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-JP-ja-GHS_2_0.pdf',
			'countries' => 'JP',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '2115',
			'name' => 'Tagmentation Buffer (2x) SDS DE de',
			'language' => 'de',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-DE-de-GHS_1_0.pdf',
			'countries' => 'DE',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '2113',
			'name' => 'Tagmentation Buffer (2x) SDS BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-BE-fr-GHS_1_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '2114',
			'name' => 'Tagmentation Buffer (2x) SDS BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-BE-nl-GHS_1_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '2117',
			'name' => 'Tagmentation Buffer (2x) SDS FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-FR-fr-GHS_1_0.pdf',
			'countries' => 'FR',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/tagmentation-buffer-2x'
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
	(int) 0 => array(
		'id' => '3162',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>
</div>
<div class="small-12 medium-4 large-4 columns"><center>
<script>// <![CDATA[
 var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } 
// ]]></script>
</center></div>
</div>
<p></p>',
		'label1' => 'Examples of use',
		'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label3' => '',
		'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'format' => '300 µl ',
		'catalog_number' => 'C01019043',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-29421',
		'type' => 'FRE',
		'search_order' => '',
		'price_EUR' => '90',
		'price_USD' => '100',
		'price_GBP' => '105',
		'price_JPY' => '14100',
		'price_CNY' => '',
		'price_AUD' => '250',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x',
		'meta_title' => 'Tagmentation Buffer 2x',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2023-06-20 18:19:03',
		'created' => '2020-12-08 16:00:14'
	),
	(int) 1 => array(
		'id' => '3163',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
		'label1' => 'Examples of use',
		'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label3' => '',
		'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'format' => '100 μl',
		'catalog_number' => 'C01019043-100',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-D001-001161',
		'type' => 'FRE',
		'search_order' => '',
		'price_EUR' => '50',
		'price_USD' => '50',
		'price_GBP' => '55',
		'price_JPY' => '7830',
		'price_CNY' => '',
		'price_AUD' => '125',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x-100',
		'meta_title' => 'Tagmentation Buffer 2x 100',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2022-11-25 12:25:10',
		'created' => '2020-12-08 16:06:10',
		'ProductsGroup' => array(
			'id' => '371',
			'product_id' => '3163',
			'group_id' => '330'
		)
	),
	(int) 2 => array(
		'id' => '3217',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
		'label1' => 'Examples of use',
		'info1' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label3' => '',
		'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'format' => '1000 µl',
		'catalog_number' => 'C01019043-1000',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-178850',
		'type' => 'FRE',
		'search_order' => '',
		'price_EUR' => '225',
		'price_USD' => '255',
		'price_GBP' => '270',
		'price_JPY' => '35245',
		'price_CNY' => '',
		'price_AUD' => '638',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x-1000',
		'meta_title' => 'Tagmentation Buffer 2x',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2023-01-04 15:29:39',
		'created' => '2022-04-13 15:31:11',
		'ProductsGroup' => array(
			'id' => '405',
			'product_id' => '3217',
			'group_id' => '330'
		)
	),
	(int) 3 => array(
		'id' => '3229',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
		'label1' => 'Examples of use',
		'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '10000 µl',
		'catalog_number' => 'C01019043-10000',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-190331',
		'type' => 'FRE',
		'search_order' => '1400, GBP 1640, USD 1500, AUD 3750, JPY ¥244,000',
		'price_EUR' => '1475',
		'price_USD' => '1585',
		'price_GBP' => '1730',
		'price_JPY' => '231060',
		'price_CNY' => '/',
		'price_AUD' => '3962',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x-10000',
		'meta_title' => 'Tagmentation Buffer 2x',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2022-11-25 12:29:09',
		'created' => '2022-11-25 11:26:45',
		'ProductsGroup' => array(
			'id' => '412',
			'product_id' => '3229',
			'group_id' => '330'
		)
	)
)
$pro = array(
	'id' => '3229',
	'antibody_id' => null,
	'name' => 'Tagmentation Buffer (2x)',
	'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
	'label1' => 'Examples of use',
	'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '10000 µl',
	'catalog_number' => 'C01019043-10000',
	'old_catalog_number' => '',
	'sf_code' => 'C01019043-190331',
	'type' => 'FRE',
	'search_order' => '1400, GBP 1640, USD 1500, AUD 3750, JPY ¥244,000',
	'price_EUR' => '1475',
	'price_USD' => '1585',
	'price_GBP' => '1730',
	'price_JPY' => '231060',
	'price_CNY' => '/',
	'price_AUD' => '3962',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '',
	'slug' => 'tagmentation-buffer-2x-10000',
	'meta_title' => 'Tagmentation Buffer 2x',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2022-11-25 12:29:09',
	'created' => '2022-11-25 11:26:45',
	'ProductsGroup' => array(
		'id' => '412',
		'product_id' => '3229',
		'group_id' => '330'
	)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
	'id' => '3229',
	'antibody_id' => null,
	'name' => 'Tagmentation Buffer (2x)',
	'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
	'label1' => 'Examples of use',
	'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '10000 µl',
	'catalog_number' => 'C01019043-10000',
	'old_catalog_number' => '',
	'sf_code' => 'C01019043-190331',
	'type' => 'FRE',
	'search_order' => '1400, GBP 1640, USD 1500, AUD 3750, JPY ¥244,000',
	'price_EUR' => '1475',
	'price_USD' => '1585',
	'price_GBP' => '1730',
	'price_JPY' => '231060',
	'price_CNY' => '/',
	'price_AUD' => '3962',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '',
	'slug' => 'tagmentation-buffer-2x-10000',
	'meta_title' => 'Tagmentation Buffer 2x',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2022-11-25 12:29:09',
	'created' => '2022-11-25 11:26:45',
	'ProductsGroup' => array(
		'id' => '412',
		'product_id' => '3229',
		'group_id' => '330'
	)
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '1115',
	'name' => 'Datasheet Tagmentation Buffer (2x) C01019043',
	'description' => '',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/kits/Datasheet_Tagmentation Buffer_2x_C01019043.pdf',
	'slug' => 'tagmentation-buffer-2x-datasheet',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2020-12-10 17:02:47',
	'created' => '2020-12-10 17:02:47',
	'ProductsDocument' => array(
		'id' => '3259',
		'product_id' => '3228',
		'document_id' => '1115'
	)
)
$sds = array(
	'id' => '2117',
	'name' => 'Tagmentation Buffer (2x) SDS FR fr',
	'language' => 'fr',
	'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-FR-fr-GHS_1_0.pdf',
	'countries' => 'FR',
	'modified' => '2021-10-12 14:27:13',
	'created' => '2021-10-12 14:27:13',
	'ProductsSafetySheet' => array(
		'id' => '4772',
		'product_id' => '3228',
		'safety_sheet_id' => '2117'
	)
)
$publication = array(
	'id' => '4660',
	'name' => 'EBF1 is continuously required for stabilizing local chromatinaccessibility in pro-B cells.',
	'authors' => 'Zolotarev  Nikolay et al.',
	'description' => '<p>The establishment of de novo chromatin accessibility in lymphoid progenitors requires the "pioneering" function of transcription factor (TF) early B cell factor 1 (EBF1), which binds to na&iuml;ve chromatin and induces accessibility by recruiting the BRG1 chromatin remodeler subunit. However, it remains unclear whether the function of EBF1 is continuously required for stabilizing local chromatin accessibility. To this end, we replaced EBF1 by EBF1-FKBP in pro-B cells, allowing the rapid degradation by adding the degradation TAG13 (dTAG13) dimerizer. EBF1 degradation results in a loss of genome-wide EBF1 occupancy and EBF1-targeted BRG1 binding. Chromatin accessibility was rapidly diminished at EBF1-binding sites with a preference for sites whose occupancy requires the pioneering activity of the C-terminal domain of EBF1. Diminished chromatin accessibility correlated with altered gene expression. Thus, continuous activity of EBF1 is required for the stable maintenance of the transcriptional and epigenetic state of pro-B cells.</p>',
	'date' => '2022-11-01',
	'pmid' => 'https://doi.org/10.1073%2Fpnas',
	'doi' => '10.1073/pnas.2210595119',
	'modified' => '2023-03-07 09:07:41',
	'created' => '2023-02-21 09:59:46',
	'ProductsPublication' => array(
		'id' => '6556',
		'product_id' => '3228',
		'publication_id' => '4660'
	)
)
$externalLink = ' <a href="https://doi.org/10.1073%2Fpnas" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'cn',
	'meta_keywords' => '',
	'meta_description' => '',
	'meta_title' => 'Tagmentation Buffer 2x',
	'product' => array(
		'Product' => array(
			'id' => '3228',
			'antibody_id' => null,
			'name' => 'Tagmentation Buffer (2x)',
			'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
			'label1' => 'Examples of use',
			'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '5000 µl',
			'catalog_number' => 'C01019043-5000',
			'old_catalog_number' => '',
			'sf_code' => 'C01019043-190330',
			'type' => 'FRE',
			'search_order' => '',
			'price_EUR' => '845',
			'price_USD' => '940',
			'price_GBP' => '990',
			'price_JPY' => '132370',
			'price_CNY' => '',
			'price_AUD' => '2350',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => false,
			'last_datasheet_update' => '',
			'slug' => 'tagmentation-buffer-2x-5000',
			'meta_title' => 'Tagmentation Buffer 2x',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-11-25 12:27:51',
			'created' => '2022-11-25 11:18:25',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(),
		'Group' => array(
			'Group' => array(
				[maximum depth reached]
			),
			'Master' => array(
				[maximum depth reached]
			),
			'Product' => array(
				[maximum depth reached]
			)
		),
		'Related' => array(),
		'Application' => array(),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	),
	'meta_canonical' => 'https://www.diagenode.com/cn/p/tagmentation-buffer-2x'
)
$language = 'cn'
$meta_keywords = ''
$meta_description = ''
$meta_title = 'Tagmentation Buffer 2x'
$product = array(
	'Product' => array(
		'id' => '3228',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
		'label1' => 'Examples of use',
		'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label3' => '',
		'info3' => '',
		'format' => '5000 µl',
		'catalog_number' => 'C01019043-5000',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-190330',
		'type' => 'FRE',
		'search_order' => '',
		'price_EUR' => '845',
		'price_USD' => '940',
		'price_GBP' => '990',
		'price_JPY' => '132370',
		'price_CNY' => '',
		'price_AUD' => '2350',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x-5000',
		'meta_title' => 'Tagmentation Buffer 2x',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2022-11-25 12:27:51',
		'created' => '2022-11-25 11:18:25',
		'locale' => 'zho'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(),
	'Group' => array(
		'Group' => array(
			'id' => '330',
			'name' => 'C01019043',
			'product_id' => '3162',
			'modified' => '2022-11-25 11:27:26',
			'created' => '2020-12-08 16:06:34'
		),
		'Master' => array(
			'id' => '3162',
			'antibody_id' => null,
			'name' => 'Tagmentation Buffer (2x)',
			'description' => '<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>
</div>
<div class="small-12 medium-4 large-4 columns"><center>
<script>// <![CDATA[
 var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } 
// ]]></script>
</center></div>
</div>
<p></p>',
			'label1' => 'Examples of use',
			'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => '',
			'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label3' => '',
			'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'format' => '300 µl ',
			'catalog_number' => 'C01019043',
			'old_catalog_number' => '',
			'sf_code' => 'C01019043-29421',
			'type' => 'FRE',
			'search_order' => '',
			'price_EUR' => '90',
			'price_USD' => '100',
			'price_GBP' => '105',
			'price_JPY' => '14100',
			'price_CNY' => '',
			'price_AUD' => '250',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '',
			'slug' => 'tagmentation-buffer-2x',
			'meta_title' => 'Tagmentation Buffer 2x',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2023-06-20 18:19:03',
			'created' => '2020-12-08 16:00:14'
		),
		'Product' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		)
	),
	'Related' => array(),
	'Application' => array(),
	'Category' => array(
		(int) 0 => array(
			'id' => '141',
			'position' => '0',
			'parent_id' => '86',
			'name' => 'Tagmentation enzymes',
			'description' => '',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'tagmentation-enzymes',
			'cookies_tag_id' => null,
			'meta_keywords' => '',
			'meta_description' => '',
			'meta_title' => 'Tagmentation enzymes',
			'modified' => '2020-03-24 17:49:07',
			'created' => '2020-03-24 16:53:13',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '1115',
			'name' => 'Datasheet Tagmentation Buffer (2x) C01019043',
			'description' => '',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/kits/Datasheet_Tagmentation Buffer_2x_C01019043.pdf',
			'slug' => 'tagmentation-buffer-2x-datasheet',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2020-12-10 17:02:47',
			'created' => '2020-12-10 17:02:47',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '1776',
			'name' => 'product/kits/kit-icon.png',
			'alt' => 'Kit icon',
			'modified' => '2019-04-09 12:06:37',
			'created' => '2018-03-15 15:51:02',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '5002',
			'name' => 'HIRA protects telomeres against R-loop-induced instability in ALT cancer cells',
			'authors' => 'Michelle Lee Lynskey et al.',
			'description' => '<section id="author-highlights-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name">Highlights</h2>
<div id="abspara0020" role="paragraph">
<div id="ulist0010" role="list">
<div id="u0010" role="listitem">
<div class="content">
<div id="p0010" role="paragraph">HIRA establishes greater telomeric chromatin accessibility after ATRX-DAXX loss</div>
</div>
</div>
<div id="u0015" role="listitem">
<div class="content">
<div id="p0015" role="paragraph">Deposition of new H3.3 by HIRA-UBN restricts telomeric ssDNA and TERRA R-loops</div>
</div>
</div>
<div id="u0020" role="listitem">
<div class="content">
<div id="p0020" role="paragraph">Unresolved TERRA R-loops block new H3.3 deposition by HIRA-UBN</div>
</div>
</div>
<div id="u0025" role="listitem">
<div class="content">
<div id="p0025" role="paragraph">CHK1 phosphorylation of H3.3 is critical to prevent ssDNA and TERRA R-loop buildup</div>
</div>
</div>
</div>
</div>
</section>
<section id="author-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name">Summary</h2>
<div id="abspara0010" role="paragraph">Inactivating mutations in chromatin modifiers, like the &alpha;-thalassemia/mental retardation, X-linked (ATRX)-death domain-associated protein (DAXX) chromatin remodeling/histone H3.3 deposition complex, drive the cancer-specific alternative lengthening of telomeres (ALT) pathway. Prior studies revealed that HIRA, another histone H3.3 chaperone, compensates for ATRX-DAXX loss at telomeres to sustain ALT cancer cell survival. How HIRA rescues telomeres from the consequences of ATRX-DAXX deficiency remains unclear. Here, using an assay for transposase-accessible chromatin using sequencing (ATAC-seq) and cleavage under targets and release using nuclease (CUT&amp;RUN), we establish that HIRA-mediated deposition of new H3.3 maintains telomeric chromatin accessibility to prevent the detrimental accumulation of nucleosome-free single-stranded DNA (ssDNA) in ATRX-DAXX-deficient ALT cells. We show that the HIRA-UBN1/UBN2 complex deposits new H3.3 to prevent TERRA R-loop buildup and transcription-replication conflicts (TRCs) at telomeres. Furthermore, HIRA-mediated H3.3 incorporation into telomeric chromatin links productive ALT to the phosphorylation of serine 31, an H3.3-specific amino acid, by Chk1. Therefore, we identify a critical role for HIRA-mediated H3.3 deposition that ensures the survival of ATRX-DAXX-deficient ALT cancer cells.</div>
</section>
<section id="graphical-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name"></h2>
</section>',
			'date' => '2024-11-26',
			'pmid' => 'https://www.cell.com/cell-reports/fulltext/S2211-1247(24)01315-9',
			'doi' => '10.1016/j.celrep.2024.114964',
			'modified' => '2024-11-12 09:41:40',
			'created' => '2024-11-12 09:41:40',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '4985',
			'name' => 'HNF1β bookmarking involves Topoisomerase 1 activation and DNA topology relaxation in mitotic chromatin',
			'authors' => 'Alessia Bagattin et al.',
			'description' => '<section id="author-highlights-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name">Highlights</h2>
<div id="abspara0020" role="paragraph">
<div id="ulist0010" role="list">
<div id="u0010" role="listitem">
<div class="content">
<div id="p0010" role="paragraph">HNF1&beta; mitotic site binding is preserved with a specific methanol/formaldehyde ChIP</div>
</div>
</div>
<div id="u0015" role="listitem">
<div class="content">
<div id="p0015" role="paragraph">BTBD2, an HNF1&beta; partner, mediates mitosis-specific interaction with TOP1</div>
</div>
</div>
<div id="u0020" role="listitem">
<div class="content">
<div id="p0020" role="paragraph">HNF1&beta; recruits TOP1 and induces DNA relaxation around bookmarked HNF1&beta; sites</div>
</div>
</div>
<div id="u0025" role="listitem">
<div class="content">
<div id="p0025" role="paragraph">An HNF1&beta; mutation, found in MODY patients, disrupts the interaction with TOP1</div>
</div>
</div>
</div>
</div>
</section>
<section id="author-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name">Summary</h2>
<div id="abspara0010" role="paragraph">HNF1&beta; (<i>HNF1B</i>) is a transcription factor frequently mutated in patients with developmental renal disease. It binds to mitotic chromatin and reactivates gene expression after mitosis, a phenomenon referred to as bookmarking. Using a crosslinking method that circumvents the artifacts of formaldehyde, we demonstrate that&nbsp;HNF1&beta; remains associated with chromatin in a sequence-specific way in both interphase and mitosis. We identify an HNF1&beta;-interacting protein, BTBD2, that enables the interaction and activation of Topoisomerase 1 (TOP1) exclusively during mitosis. Our study identifies a shared microhomology domain between HNF1&beta; and TOP1, where a mutation, found in &ldquo;maturity onset diabetes of the young&rdquo; patients, disrupts their interaction. Importantly, HNF1&beta; recruits TOP1 and induces DNA relaxation around HNF1&beta; mitotic chromatin sites, elucidating its crucial role in chromatin remodeling and gene reactivation after mitotic exit. These findings shed light on how HNF1&beta; reactivates target gene expression after mitosis, providing insights into its crucial role in maintenance of cellular identity.</div>
</section>',
			'date' => '2024-10-08',
			'pmid' => 'https://www.cell.com/cell-reports/fulltext/S2211-1247(24)01156-2',
			'doi' => '10.1016/j.celrep.2024.114805',
			'modified' => '2024-10-14 09:04:44',
			'created' => '2024-10-14 09:04:44',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '4926',
			'name' => 'High-throughput sequencing of insect specimens with sub-optimal DNA preservation using a practical, plate-based Illumina-compatible Tn5 transposase library preparation method',
			'authors' => 'Cobb L. et all.',
			'description' => '<p><span>Entomological sampling and storage conditions often prioritise efficiency, practicality and conservation of morphological characteristics, and may therefore be suboptimal for DNA preservation. This practice can impact downstream molecular applications, such as the generation of high-throughput genomic libraries, which often requires substantial DNA input amounts. Here, we use a practical Tn5 transposase tagmentation-based library preparation method optimised for 96-well plates and low yield DNA extracts from insect legs that were stored under sub-optimal conditions for DNA preservation. The samples were kept in field vehicles for extended periods of time, before long-term storage in ethanol in the freezer, or dry at room temperature. By reducing DNA input to 6ng, more samples with sub-optimal DNA yields could be processed. We matched this low DNA input with a 6-fold dilution of a commercially available tagmentation enzyme, significantly reducing library preparation costs. Costs and workload were further suppressed by direct post-amplification pooling of individual libraries. We generated medium coverage (&gt;3-fold) genomes for 88 out of 90 specimens, with an average of approximately 10-fold coverage. While samples stored in ethanol yielded significantly less DNA compared to those which were stored dry, these samples had superior sequencing statistics, with longer sequencing reads and higher rates of endogenous DNA. Furthermore, we find that the efficiency of tagmentation-based library preparation can be improved by a thorough post-amplification bead clean-up which selects against both short and large DNA fragments. By opening opportunities for the use of sub-optimally preserved, low yield DNA extracts, we broaden the scope of whole genome studies of insect specimens. We therefore expect these results and this protocol to be valuable for a range of applications in the field of entomology.</span></p>',
			'date' => '2024-03-22',
			'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/38517905/',
			'doi' => '10.1371/journal.pone.0300865',
			'modified' => '2024-03-25 11:15:06',
			'created' => '2024-03-25 11:15:06',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '4923',
			'name' => 'On the identification of differentially-active transcription factors from ATAC-seq data',
			'authors' => 'Gerbaldo F. et al.',
			'description' => '<p><span>ATAC-seq has emerged as a rich epigenome profiling technique, and is commonly used to identify Transcription Factors (TFs) underlying given phenomena. A number of methods can be used to identify differentially-active TFs through the accessibility of their DNA-binding motif, however little is known on the best approaches for doing so. Here we benchmark several such methods using a combination of curated datasets with various forms of short-term perturbations on known TFs, as well as semi-simulations. We include both methods specifically designed for this type of data as well as some that can be repurposed for it. We also investigate variations to these methods, and identify three particularly promising approaches (chromVAR-limma with critical adjustments, monaLisa and a combination of GC smooth quantile normalization and multivariate modeling). We further investigate the specific use of nucleosome-free fragments, the combination of top methods, and the impact of technical variation. Finally, we illustrate the use of the top methods on a novel dataset to characterize the impact on DNA accessibility of TRAnscription Factor TArgeting Chimeras (TRAFTAC), which can deplete TFs &ndash; in our case NFkB &ndash; at the protein level.</span></p>',
			'date' => '2024-03-10',
			'pmid' => 'https://www.biorxiv.org/content/10.1101/2024.03.06.583825v2',
			'doi' => 'https://doi.org/10.1101/2024.03.06.583825',
			'modified' => '2024-03-13 17:04:33',
			'created' => '2024-03-13 17:04:33',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '4878',
			'name' => 'ARID1A governs the silencing of sex-linked transcription during male meiosis in the mouse',
			'authors' => 'Menon D.U. et al.',
			'description' => '<p><span>We present evidence implicating the BAF (BRG1/BRM Associated Factor) chromatin remodeler in meiotic sex chromosome inactivation (MSCI). By immunofluorescence (IF), the putative BAF DNA binding subunit, ARID1A (AT-rich Interaction Domain 1a), appeared enriched on the male sex chromosomes during diplonema of meiosis I. The germ cell-specific depletion of ARID1A resulted in a pachynema arrest and failure to repress sex-linked genes, indicating a defective MSCI. Consistent with this defect, mutant sex chromosomes displayed an abnormal presence of elongating RNA polymerase II coupled with an overall increase in chromatin accessibility detectable by ATAC-seq. By investigating potential mechanisms underlying these anomalies, we identified a role for ARID1A in promoting the preferential enrichment of the histone variant, H3.3, on the sex chromosomes, a known hallmark of MSCI. Without ARID1A, the sex chromosomes appeared depleted of H3.3 at levels resembling autosomes. Higher resolution analyses by CUT&amp;RUN revealed shifts in sex-linked H3.3 associations from discrete intergenic sites and broader gene-body domains to promoters in response to the loss of ARID1A. Several sex-linked sites displayed ectopic H3.3 occupancy that did not co-localize with DMC1 (DNA Meiotic Recombinase 1). This observation suggests a requirement for ARID1A in DMC1 localization to the asynapsed sex chromatids. We conclude that ARID1A-directed H3.3 localization influences meiotic sex chromosome gene regulation and DNA repair.</span></p>',
			'date' => '2023-09-28',
			'pmid' => 'https://www.biorxiv.org/content/10.1101/2023.05.25.542290v2.abstract',
			'doi' => 'https://doi.org/10.1101/2023.05.25.542290',
			'modified' => '2023-11-10 14:53:09',
			'created' => '2023-11-10 14:53:09',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '4817',
			'name' => 'YAP/BRD4-controlled ROR1 promotes tumor-initiating cells andhyperproliferation in pancreatic cancer.',
			'authors' => 'Yamazaki  M. et al.',
			'description' => '<p><span>Tumor-initiating cells are major drivers of chemoresistance and attractive targets for cancer therapy, however, their identity in human pancreatic ductal adenocarcinoma (PDAC) and the key molecules underlying their traits remain poorly understood. Here, we show that a cellular subpopulation with partial epithelial-mesenchymal transition (EMT)-like signature marked by high expression of receptor tyrosine kinase-like orphan receptor 1 (ROR1) is the origin of heterogeneous tumor cells in PDAC. We demonstrate that ROR1 depletion suppresses tumor growth, recurrence after chemotherapy, and metastasis. Mechanistically, ROR1 induces the expression of Aurora kinase B (AURKB) by activating E2F through c-Myc to enhance PDAC proliferation. Furthermore, epigenomic analyses reveal that ROR1 is transcriptionally dependent on YAP/BRD4 binding at the enhancer region, and targeting this pathway reduces ROR1 expression and prevents PDAC growth. Collectively, our findings reveal a critical role for ROR1high cells as tumor-initiating cells and the functional importance of ROR1 in PDAC progression, thereby highlighting its therapeutic targetability.</span></p>',
			'date' => '2023-04-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37096681',
			'doi' => '10.15252/embj.2022112614',
			'modified' => '2023-06-15 10:06:12',
			'created' => '2023-06-13 21:11:31',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '4742',
			'name' => 'A neurodevelopmental epigenetic programme mediated bySMARCD3-DAB1-Reelin signalling is hijacked to promote medulloblastomametastasis.',
			'authors' => 'Zou  Han et al.',
			'description' => '<p>How abnormal neurodevelopment relates to the tumour aggressiveness of medulloblastoma (MB), the most common type of embryonal tumour, remains elusive. Here we uncover a neurodevelopmental epigenomic programme that is hijacked to induce MB metastatic dissemination. Unsupervised analyses of integrated publicly available datasets with our newly generated data reveal that SMARCD3 (also known as BAF60C) regulates Disabled&acirc;&euro;&permil;1 (DAB1)-mediated Reelin signalling in Purkinje cell migration and MB metastasis by orchestrating cis-regulatory elements at the DAB1 locus. We further identify that a core set of transcription factors, enhancer of zeste homologue&acirc;&euro;&permil;2 (EZH2) and nuclear factor&acirc;&euro;&permil;I&acirc;&euro;&permil;X (NFIX), coordinates with the cis-regulatory elements at the SMARCD3 locus to form a chromatin hub to control SMARCD3 expression in the developing cerebellum and in metastatic MB. Increased SMARCD3 expression activates Reelin-DAB1-mediated Src kinase signalling, which results in a MB response to Src inhibition. These data deepen our understanding of how neurodevelopmental programming influences disease progression and provide a potential therapeutic option for patients with MB.</p>',
			'date' => '2023-02-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36849558',
			'doi' => '10.1038/s41556-023-01093-0',
			'modified' => '2023-03-14 09:41:24',
			'created' => '2023-03-02 17:27:08',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '4660',
			'name' => 'EBF1 is continuously required for stabilizing local chromatinaccessibility in pro-B cells.',
			'authors' => 'Zolotarev  Nikolay et al.',
			'description' => '<p>The establishment of de novo chromatin accessibility in lymphoid progenitors requires the "pioneering" function of transcription factor (TF) early B cell factor 1 (EBF1), which binds to na&iuml;ve chromatin and induces accessibility by recruiting the BRG1 chromatin remodeler subunit. However, it remains unclear whether the function of EBF1 is continuously required for stabilizing local chromatin accessibility. To this end, we replaced EBF1 by EBF1-FKBP in pro-B cells, allowing the rapid degradation by adding the degradation TAG13 (dTAG13) dimerizer. EBF1 degradation results in a loss of genome-wide EBF1 occupancy and EBF1-targeted BRG1 binding. Chromatin accessibility was rapidly diminished at EBF1-binding sites with a preference for sites whose occupancy requires the pioneering activity of the C-terminal domain of EBF1. Diminished chromatin accessibility correlated with altered gene expression. Thus, continuous activity of EBF1 is required for the stable maintenance of the transcriptional and epigenetic state of pro-B cells.</p>',
			'date' => '2022-11-01',
			'pmid' => 'https://doi.org/10.1073%2Fpnas',
			'doi' => '10.1073/pnas.2210595119',
			'modified' => '2023-03-07 09:07:41',
			'created' => '2023-02-21 09:59:46',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '2118',
			'name' => 'Tagmentation Buffer (2x) SDS GB en',
			'language' => 'en',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-GB-en-GHS_1_0.pdf',
			'countries' => 'GB',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '2120',
			'name' => 'Tagmentation Buffer (2x) SDS US en',
			'language' => 'en',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-US-en-GHS_1_0.pdf',
			'countries' => 'US',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '2116',
			'name' => 'Tagmentation Buffer (2x) SDS ES es',
			'language' => 'es',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-ES-es-GHS_1_0.pdf',
			'countries' => 'ES',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '2119',
			'name' => 'Tagmentation Buffer (2x) SDS JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-JP-ja-GHS_2_0.pdf',
			'countries' => 'JP',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '2115',
			'name' => 'Tagmentation Buffer (2x) SDS DE de',
			'language' => 'de',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-DE-de-GHS_1_0.pdf',
			'countries' => 'DE',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '2113',
			'name' => 'Tagmentation Buffer (2x) SDS BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-BE-fr-GHS_1_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '2114',
			'name' => 'Tagmentation Buffer (2x) SDS BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-BE-nl-GHS_1_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '2117',
			'name' => 'Tagmentation Buffer (2x) SDS FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-FR-fr-GHS_1_0.pdf',
			'countries' => 'FR',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/tagmentation-buffer-2x'
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
	(int) 0 => array(
		'id' => '3162',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>
</div>
<div class="small-12 medium-4 large-4 columns"><center>
<script>// <![CDATA[
 var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } 
// ]]></script>
</center></div>
</div>
<p></p>',
		'label1' => 'Examples of use',
		'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label3' => '',
		'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'format' => '300 µl ',
		'catalog_number' => 'C01019043',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-29421',
		'type' => 'FRE',
		'search_order' => '',
		'price_EUR' => '90',
		'price_USD' => '100',
		'price_GBP' => '105',
		'price_JPY' => '14100',
		'price_CNY' => '',
		'price_AUD' => '250',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x',
		'meta_title' => 'Tagmentation Buffer 2x',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2023-06-20 18:19:03',
		'created' => '2020-12-08 16:00:14'
	),
	(int) 1 => array(
		'id' => '3163',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
		'label1' => 'Examples of use',
		'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label3' => '',
		'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'format' => '100 μl',
		'catalog_number' => 'C01019043-100',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-D001-001161',
		'type' => 'FRE',
		'search_order' => '',
		'price_EUR' => '50',
		'price_USD' => '50',
		'price_GBP' => '55',
		'price_JPY' => '7830',
		'price_CNY' => '',
		'price_AUD' => '125',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x-100',
		'meta_title' => 'Tagmentation Buffer 2x 100',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2022-11-25 12:25:10',
		'created' => '2020-12-08 16:06:10',
		'ProductsGroup' => array(
			'id' => '371',
			'product_id' => '3163',
			'group_id' => '330'
		)
	),
	(int) 2 => array(
		'id' => '3217',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
		'label1' => 'Examples of use',
		'info1' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label3' => '',
		'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'format' => '1000 µl',
		'catalog_number' => 'C01019043-1000',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-178850',
		'type' => 'FRE',
		'search_order' => '',
		'price_EUR' => '225',
		'price_USD' => '255',
		'price_GBP' => '270',
		'price_JPY' => '35245',
		'price_CNY' => '',
		'price_AUD' => '638',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x-1000',
		'meta_title' => 'Tagmentation Buffer 2x',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2023-01-04 15:29:39',
		'created' => '2022-04-13 15:31:11',
		'ProductsGroup' => array(
			'id' => '405',
			'product_id' => '3217',
			'group_id' => '330'
		)
	),
	(int) 3 => array(
		'id' => '3229',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
		'label1' => 'Examples of use',
		'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '10000 µl',
		'catalog_number' => 'C01019043-10000',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-190331',
		'type' => 'FRE',
		'search_order' => '1400, GBP 1640, USD 1500, AUD 3750, JPY ¥244,000',
		'price_EUR' => '1475',
		'price_USD' => '1585',
		'price_GBP' => '1730',
		'price_JPY' => '231060',
		'price_CNY' => '/',
		'price_AUD' => '3962',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x-10000',
		'meta_title' => 'Tagmentation Buffer 2x',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2022-11-25 12:29:09',
		'created' => '2022-11-25 11:26:45',
		'ProductsGroup' => array(
			'id' => '412',
			'product_id' => '3229',
			'group_id' => '330'
		)
	)
)
$pro = array(
	'id' => '3229',
	'antibody_id' => null,
	'name' => 'Tagmentation Buffer (2x)',
	'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
	'label1' => 'Examples of use',
	'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '10000 µl',
	'catalog_number' => 'C01019043-10000',
	'old_catalog_number' => '',
	'sf_code' => 'C01019043-190331',
	'type' => 'FRE',
	'search_order' => '1400, GBP 1640, USD 1500, AUD 3750, JPY ¥244,000',
	'price_EUR' => '1475',
	'price_USD' => '1585',
	'price_GBP' => '1730',
	'price_JPY' => '231060',
	'price_CNY' => '/',
	'price_AUD' => '3962',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '',
	'slug' => 'tagmentation-buffer-2x-10000',
	'meta_title' => 'Tagmentation Buffer 2x',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2022-11-25 12:29:09',
	'created' => '2022-11-25 11:26:45',
	'ProductsGroup' => array(
		'id' => '412',
		'product_id' => '3229',
		'group_id' => '330'
	)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
	'id' => '3229',
	'antibody_id' => null,
	'name' => 'Tagmentation Buffer (2x)',
	'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
	'label1' => 'Examples of use',
	'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '10000 µl',
	'catalog_number' => 'C01019043-10000',
	'old_catalog_number' => '',
	'sf_code' => 'C01019043-190331',
	'type' => 'FRE',
	'search_order' => '1400, GBP 1640, USD 1500, AUD 3750, JPY ¥244,000',
	'price_EUR' => '1475',
	'price_USD' => '1585',
	'price_GBP' => '1730',
	'price_JPY' => '231060',
	'price_CNY' => '/',
	'price_AUD' => '3962',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '',
	'slug' => 'tagmentation-buffer-2x-10000',
	'meta_title' => 'Tagmentation Buffer 2x',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2022-11-25 12:29:09',
	'created' => '2022-11-25 11:26:45',
	'ProductsGroup' => array(
		'id' => '412',
		'product_id' => '3229',
		'group_id' => '330'
	)
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '1115',
	'name' => 'Datasheet Tagmentation Buffer (2x) C01019043',
	'description' => '',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/kits/Datasheet_Tagmentation Buffer_2x_C01019043.pdf',
	'slug' => 'tagmentation-buffer-2x-datasheet',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2020-12-10 17:02:47',
	'created' => '2020-12-10 17:02:47',
	'ProductsDocument' => array(
		'id' => '3259',
		'product_id' => '3228',
		'document_id' => '1115'
	)
)
$sds = array(
	'id' => '2117',
	'name' => 'Tagmentation Buffer (2x) SDS FR fr',
	'language' => 'fr',
	'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-FR-fr-GHS_1_0.pdf',
	'countries' => 'FR',
	'modified' => '2021-10-12 14:27:13',
	'created' => '2021-10-12 14:27:13',
	'ProductsSafetySheet' => array(
		'id' => '4772',
		'product_id' => '3228',
		'safety_sheet_id' => '2117'
	)
)
$publication = array(
	'id' => '4660',
	'name' => 'EBF1 is continuously required for stabilizing local chromatinaccessibility in pro-B cells.',
	'authors' => 'Zolotarev  Nikolay et al.',
	'description' => '<p>The establishment of de novo chromatin accessibility in lymphoid progenitors requires the "pioneering" function of transcription factor (TF) early B cell factor 1 (EBF1), which binds to na&iuml;ve chromatin and induces accessibility by recruiting the BRG1 chromatin remodeler subunit. However, it remains unclear whether the function of EBF1 is continuously required for stabilizing local chromatin accessibility. To this end, we replaced EBF1 by EBF1-FKBP in pro-B cells, allowing the rapid degradation by adding the degradation TAG13 (dTAG13) dimerizer. EBF1 degradation results in a loss of genome-wide EBF1 occupancy and EBF1-targeted BRG1 binding. Chromatin accessibility was rapidly diminished at EBF1-binding sites with a preference for sites whose occupancy requires the pioneering activity of the C-terminal domain of EBF1. Diminished chromatin accessibility correlated with altered gene expression. Thus, continuous activity of EBF1 is required for the stable maintenance of the transcriptional and epigenetic state of pro-B cells.</p>',
	'date' => '2022-11-01',
	'pmid' => 'https://doi.org/10.1073%2Fpnas',
	'doi' => '10.1073/pnas.2210595119',
	'modified' => '2023-03-07 09:07:41',
	'created' => '2023-02-21 09:59:46',
	'ProductsPublication' => array(
		'id' => '6556',
		'product_id' => '3228',
		'publication_id' => '4660'
	)
)
$externalLink = ' <a href="https://doi.org/10.1073%2Fpnas" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'cn',
	'meta_keywords' => '',
	'meta_description' => '',
	'meta_title' => 'Tagmentation Buffer 2x',
	'product' => array(
		'Product' => array(
			'id' => '3228',
			'antibody_id' => null,
			'name' => 'Tagmentation Buffer (2x)',
			'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
			'label1' => 'Examples of use',
			'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '5000 µl',
			'catalog_number' => 'C01019043-5000',
			'old_catalog_number' => '',
			'sf_code' => 'C01019043-190330',
			'type' => 'FRE',
			'search_order' => '',
			'price_EUR' => '845',
			'price_USD' => '940',
			'price_GBP' => '990',
			'price_JPY' => '132370',
			'price_CNY' => '',
			'price_AUD' => '2350',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => false,
			'last_datasheet_update' => '',
			'slug' => 'tagmentation-buffer-2x-5000',
			'meta_title' => 'Tagmentation Buffer 2x',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-11-25 12:27:51',
			'created' => '2022-11-25 11:18:25',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(),
		'Group' => array(
			'Group' => array(
				[maximum depth reached]
			),
			'Master' => array(
				[maximum depth reached]
			),
			'Product' => array(
				[maximum depth reached]
			)
		),
		'Related' => array(),
		'Application' => array(),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	),
	'meta_canonical' => 'https://www.diagenode.com/cn/p/tagmentation-buffer-2x'
)
$language = 'cn'
$meta_keywords = ''
$meta_description = ''
$meta_title = 'Tagmentation Buffer 2x'
$product = array(
	'Product' => array(
		'id' => '3228',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
		'label1' => 'Examples of use',
		'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label3' => '',
		'info3' => '',
		'format' => '5000 µl',
		'catalog_number' => 'C01019043-5000',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-190330',
		'type' => 'FRE',
		'search_order' => '',
		'price_EUR' => '845',
		'price_USD' => '940',
		'price_GBP' => '990',
		'price_JPY' => '132370',
		'price_CNY' => '',
		'price_AUD' => '2350',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x-5000',
		'meta_title' => 'Tagmentation Buffer 2x',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2022-11-25 12:27:51',
		'created' => '2022-11-25 11:18:25',
		'locale' => 'zho'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(),
	'Group' => array(
		'Group' => array(
			'id' => '330',
			'name' => 'C01019043',
			'product_id' => '3162',
			'modified' => '2022-11-25 11:27:26',
			'created' => '2020-12-08 16:06:34'
		),
		'Master' => array(
			'id' => '3162',
			'antibody_id' => null,
			'name' => 'Tagmentation Buffer (2x)',
			'description' => '<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>
</div>
<div class="small-12 medium-4 large-4 columns"><center>
<script>// <![CDATA[
 var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } 
// ]]></script>
</center></div>
</div>
<p></p>',
			'label1' => 'Examples of use',
			'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => '',
			'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label3' => '',
			'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'format' => '300 µl ',
			'catalog_number' => 'C01019043',
			'old_catalog_number' => '',
			'sf_code' => 'C01019043-29421',
			'type' => 'FRE',
			'search_order' => '',
			'price_EUR' => '90',
			'price_USD' => '100',
			'price_GBP' => '105',
			'price_JPY' => '14100',
			'price_CNY' => '',
			'price_AUD' => '250',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '',
			'slug' => 'tagmentation-buffer-2x',
			'meta_title' => 'Tagmentation Buffer 2x',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2023-06-20 18:19:03',
			'created' => '2020-12-08 16:00:14'
		),
		'Product' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		)
	),
	'Related' => array(),
	'Application' => array(),
	'Category' => array(
		(int) 0 => array(
			'id' => '141',
			'position' => '0',
			'parent_id' => '86',
			'name' => 'Tagmentation enzymes',
			'description' => '',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'tagmentation-enzymes',
			'cookies_tag_id' => null,
			'meta_keywords' => '',
			'meta_description' => '',
			'meta_title' => 'Tagmentation enzymes',
			'modified' => '2020-03-24 17:49:07',
			'created' => '2020-03-24 16:53:13',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '1115',
			'name' => 'Datasheet Tagmentation Buffer (2x) C01019043',
			'description' => '',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/kits/Datasheet_Tagmentation Buffer_2x_C01019043.pdf',
			'slug' => 'tagmentation-buffer-2x-datasheet',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2020-12-10 17:02:47',
			'created' => '2020-12-10 17:02:47',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '1776',
			'name' => 'product/kits/kit-icon.png',
			'alt' => 'Kit icon',
			'modified' => '2019-04-09 12:06:37',
			'created' => '2018-03-15 15:51:02',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '5002',
			'name' => 'HIRA protects telomeres against R-loop-induced instability in ALT cancer cells',
			'authors' => 'Michelle Lee Lynskey et al.',
			'description' => '<section id="author-highlights-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name">Highlights</h2>
<div id="abspara0020" role="paragraph">
<div id="ulist0010" role="list">
<div id="u0010" role="listitem">
<div class="content">
<div id="p0010" role="paragraph">HIRA establishes greater telomeric chromatin accessibility after ATRX-DAXX loss</div>
</div>
</div>
<div id="u0015" role="listitem">
<div class="content">
<div id="p0015" role="paragraph">Deposition of new H3.3 by HIRA-UBN restricts telomeric ssDNA and TERRA R-loops</div>
</div>
</div>
<div id="u0020" role="listitem">
<div class="content">
<div id="p0020" role="paragraph">Unresolved TERRA R-loops block new H3.3 deposition by HIRA-UBN</div>
</div>
</div>
<div id="u0025" role="listitem">
<div class="content">
<div id="p0025" role="paragraph">CHK1 phosphorylation of H3.3 is critical to prevent ssDNA and TERRA R-loop buildup</div>
</div>
</div>
</div>
</div>
</section>
<section id="author-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name">Summary</h2>
<div id="abspara0010" role="paragraph">Inactivating mutations in chromatin modifiers, like the &alpha;-thalassemia/mental retardation, X-linked (ATRX)-death domain-associated protein (DAXX) chromatin remodeling/histone H3.3 deposition complex, drive the cancer-specific alternative lengthening of telomeres (ALT) pathway. Prior studies revealed that HIRA, another histone H3.3 chaperone, compensates for ATRX-DAXX loss at telomeres to sustain ALT cancer cell survival. How HIRA rescues telomeres from the consequences of ATRX-DAXX deficiency remains unclear. Here, using an assay for transposase-accessible chromatin using sequencing (ATAC-seq) and cleavage under targets and release using nuclease (CUT&amp;RUN), we establish that HIRA-mediated deposition of new H3.3 maintains telomeric chromatin accessibility to prevent the detrimental accumulation of nucleosome-free single-stranded DNA (ssDNA) in ATRX-DAXX-deficient ALT cells. We show that the HIRA-UBN1/UBN2 complex deposits new H3.3 to prevent TERRA R-loop buildup and transcription-replication conflicts (TRCs) at telomeres. Furthermore, HIRA-mediated H3.3 incorporation into telomeric chromatin links productive ALT to the phosphorylation of serine 31, an H3.3-specific amino acid, by Chk1. Therefore, we identify a critical role for HIRA-mediated H3.3 deposition that ensures the survival of ATRX-DAXX-deficient ALT cancer cells.</div>
</section>
<section id="graphical-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name"></h2>
</section>',
			'date' => '2024-11-26',
			'pmid' => 'https://www.cell.com/cell-reports/fulltext/S2211-1247(24)01315-9',
			'doi' => '10.1016/j.celrep.2024.114964',
			'modified' => '2024-11-12 09:41:40',
			'created' => '2024-11-12 09:41:40',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '4985',
			'name' => 'HNF1β bookmarking involves Topoisomerase 1 activation and DNA topology relaxation in mitotic chromatin',
			'authors' => 'Alessia Bagattin et al.',
			'description' => '<section id="author-highlights-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name">Highlights</h2>
<div id="abspara0020" role="paragraph">
<div id="ulist0010" role="list">
<div id="u0010" role="listitem">
<div class="content">
<div id="p0010" role="paragraph">HNF1&beta; mitotic site binding is preserved with a specific methanol/formaldehyde ChIP</div>
</div>
</div>
<div id="u0015" role="listitem">
<div class="content">
<div id="p0015" role="paragraph">BTBD2, an HNF1&beta; partner, mediates mitosis-specific interaction with TOP1</div>
</div>
</div>
<div id="u0020" role="listitem">
<div class="content">
<div id="p0020" role="paragraph">HNF1&beta; recruits TOP1 and induces DNA relaxation around bookmarked HNF1&beta; sites</div>
</div>
</div>
<div id="u0025" role="listitem">
<div class="content">
<div id="p0025" role="paragraph">An HNF1&beta; mutation, found in MODY patients, disrupts the interaction with TOP1</div>
</div>
</div>
</div>
</div>
</section>
<section id="author-abstract" property="abstract" typeof="Text" role="doc-abstract">
<h2 property="name">Summary</h2>
<div id="abspara0010" role="paragraph">HNF1&beta; (<i>HNF1B</i>) is a transcription factor frequently mutated in patients with developmental renal disease. It binds to mitotic chromatin and reactivates gene expression after mitosis, a phenomenon referred to as bookmarking. Using a crosslinking method that circumvents the artifacts of formaldehyde, we demonstrate that&nbsp;HNF1&beta; remains associated with chromatin in a sequence-specific way in both interphase and mitosis. We identify an HNF1&beta;-interacting protein, BTBD2, that enables the interaction and activation of Topoisomerase 1 (TOP1) exclusively during mitosis. Our study identifies a shared microhomology domain between HNF1&beta; and TOP1, where a mutation, found in &ldquo;maturity onset diabetes of the young&rdquo; patients, disrupts their interaction. Importantly, HNF1&beta; recruits TOP1 and induces DNA relaxation around HNF1&beta; mitotic chromatin sites, elucidating its crucial role in chromatin remodeling and gene reactivation after mitotic exit. These findings shed light on how HNF1&beta; reactivates target gene expression after mitosis, providing insights into its crucial role in maintenance of cellular identity.</div>
</section>',
			'date' => '2024-10-08',
			'pmid' => 'https://www.cell.com/cell-reports/fulltext/S2211-1247(24)01156-2',
			'doi' => '10.1016/j.celrep.2024.114805',
			'modified' => '2024-10-14 09:04:44',
			'created' => '2024-10-14 09:04:44',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '4926',
			'name' => 'High-throughput sequencing of insect specimens with sub-optimal DNA preservation using a practical, plate-based Illumina-compatible Tn5 transposase library preparation method',
			'authors' => 'Cobb L. et all.',
			'description' => '<p><span>Entomological sampling and storage conditions often prioritise efficiency, practicality and conservation of morphological characteristics, and may therefore be suboptimal for DNA preservation. This practice can impact downstream molecular applications, such as the generation of high-throughput genomic libraries, which often requires substantial DNA input amounts. Here, we use a practical Tn5 transposase tagmentation-based library preparation method optimised for 96-well plates and low yield DNA extracts from insect legs that were stored under sub-optimal conditions for DNA preservation. The samples were kept in field vehicles for extended periods of time, before long-term storage in ethanol in the freezer, or dry at room temperature. By reducing DNA input to 6ng, more samples with sub-optimal DNA yields could be processed. We matched this low DNA input with a 6-fold dilution of a commercially available tagmentation enzyme, significantly reducing library preparation costs. Costs and workload were further suppressed by direct post-amplification pooling of individual libraries. We generated medium coverage (&gt;3-fold) genomes for 88 out of 90 specimens, with an average of approximately 10-fold coverage. While samples stored in ethanol yielded significantly less DNA compared to those which were stored dry, these samples had superior sequencing statistics, with longer sequencing reads and higher rates of endogenous DNA. Furthermore, we find that the efficiency of tagmentation-based library preparation can be improved by a thorough post-amplification bead clean-up which selects against both short and large DNA fragments. By opening opportunities for the use of sub-optimally preserved, low yield DNA extracts, we broaden the scope of whole genome studies of insect specimens. We therefore expect these results and this protocol to be valuable for a range of applications in the field of entomology.</span></p>',
			'date' => '2024-03-22',
			'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/38517905/',
			'doi' => '10.1371/journal.pone.0300865',
			'modified' => '2024-03-25 11:15:06',
			'created' => '2024-03-25 11:15:06',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '4923',
			'name' => 'On the identification of differentially-active transcription factors from ATAC-seq data',
			'authors' => 'Gerbaldo F. et al.',
			'description' => '<p><span>ATAC-seq has emerged as a rich epigenome profiling technique, and is commonly used to identify Transcription Factors (TFs) underlying given phenomena. A number of methods can be used to identify differentially-active TFs through the accessibility of their DNA-binding motif, however little is known on the best approaches for doing so. Here we benchmark several such methods using a combination of curated datasets with various forms of short-term perturbations on known TFs, as well as semi-simulations. We include both methods specifically designed for this type of data as well as some that can be repurposed for it. We also investigate variations to these methods, and identify three particularly promising approaches (chromVAR-limma with critical adjustments, monaLisa and a combination of GC smooth quantile normalization and multivariate modeling). We further investigate the specific use of nucleosome-free fragments, the combination of top methods, and the impact of technical variation. Finally, we illustrate the use of the top methods on a novel dataset to characterize the impact on DNA accessibility of TRAnscription Factor TArgeting Chimeras (TRAFTAC), which can deplete TFs &ndash; in our case NFkB &ndash; at the protein level.</span></p>',
			'date' => '2024-03-10',
			'pmid' => 'https://www.biorxiv.org/content/10.1101/2024.03.06.583825v2',
			'doi' => 'https://doi.org/10.1101/2024.03.06.583825',
			'modified' => '2024-03-13 17:04:33',
			'created' => '2024-03-13 17:04:33',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '4878',
			'name' => 'ARID1A governs the silencing of sex-linked transcription during male meiosis in the mouse',
			'authors' => 'Menon D.U. et al.',
			'description' => '<p><span>We present evidence implicating the BAF (BRG1/BRM Associated Factor) chromatin remodeler in meiotic sex chromosome inactivation (MSCI). By immunofluorescence (IF), the putative BAF DNA binding subunit, ARID1A (AT-rich Interaction Domain 1a), appeared enriched on the male sex chromosomes during diplonema of meiosis I. The germ cell-specific depletion of ARID1A resulted in a pachynema arrest and failure to repress sex-linked genes, indicating a defective MSCI. Consistent with this defect, mutant sex chromosomes displayed an abnormal presence of elongating RNA polymerase II coupled with an overall increase in chromatin accessibility detectable by ATAC-seq. By investigating potential mechanisms underlying these anomalies, we identified a role for ARID1A in promoting the preferential enrichment of the histone variant, H3.3, on the sex chromosomes, a known hallmark of MSCI. Without ARID1A, the sex chromosomes appeared depleted of H3.3 at levels resembling autosomes. Higher resolution analyses by CUT&amp;RUN revealed shifts in sex-linked H3.3 associations from discrete intergenic sites and broader gene-body domains to promoters in response to the loss of ARID1A. Several sex-linked sites displayed ectopic H3.3 occupancy that did not co-localize with DMC1 (DNA Meiotic Recombinase 1). This observation suggests a requirement for ARID1A in DMC1 localization to the asynapsed sex chromatids. We conclude that ARID1A-directed H3.3 localization influences meiotic sex chromosome gene regulation and DNA repair.</span></p>',
			'date' => '2023-09-28',
			'pmid' => 'https://www.biorxiv.org/content/10.1101/2023.05.25.542290v2.abstract',
			'doi' => 'https://doi.org/10.1101/2023.05.25.542290',
			'modified' => '2023-11-10 14:53:09',
			'created' => '2023-11-10 14:53:09',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '4817',
			'name' => 'YAP/BRD4-controlled ROR1 promotes tumor-initiating cells andhyperproliferation in pancreatic cancer.',
			'authors' => 'Yamazaki  M. et al.',
			'description' => '<p><span>Tumor-initiating cells are major drivers of chemoresistance and attractive targets for cancer therapy, however, their identity in human pancreatic ductal adenocarcinoma (PDAC) and the key molecules underlying their traits remain poorly understood. Here, we show that a cellular subpopulation with partial epithelial-mesenchymal transition (EMT)-like signature marked by high expression of receptor tyrosine kinase-like orphan receptor 1 (ROR1) is the origin of heterogeneous tumor cells in PDAC. We demonstrate that ROR1 depletion suppresses tumor growth, recurrence after chemotherapy, and metastasis. Mechanistically, ROR1 induces the expression of Aurora kinase B (AURKB) by activating E2F through c-Myc to enhance PDAC proliferation. Furthermore, epigenomic analyses reveal that ROR1 is transcriptionally dependent on YAP/BRD4 binding at the enhancer region, and targeting this pathway reduces ROR1 expression and prevents PDAC growth. Collectively, our findings reveal a critical role for ROR1high cells as tumor-initiating cells and the functional importance of ROR1 in PDAC progression, thereby highlighting its therapeutic targetability.</span></p>',
			'date' => '2023-04-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37096681',
			'doi' => '10.15252/embj.2022112614',
			'modified' => '2023-06-15 10:06:12',
			'created' => '2023-06-13 21:11:31',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '4742',
			'name' => 'A neurodevelopmental epigenetic programme mediated bySMARCD3-DAB1-Reelin signalling is hijacked to promote medulloblastomametastasis.',
			'authors' => 'Zou  Han et al.',
			'description' => '<p>How abnormal neurodevelopment relates to the tumour aggressiveness of medulloblastoma (MB), the most common type of embryonal tumour, remains elusive. Here we uncover a neurodevelopmental epigenomic programme that is hijacked to induce MB metastatic dissemination. Unsupervised analyses of integrated publicly available datasets with our newly generated data reveal that SMARCD3 (also known as BAF60C) regulates Disabled&acirc;&euro;&permil;1 (DAB1)-mediated Reelin signalling in Purkinje cell migration and MB metastasis by orchestrating cis-regulatory elements at the DAB1 locus. We further identify that a core set of transcription factors, enhancer of zeste homologue&acirc;&euro;&permil;2 (EZH2) and nuclear factor&acirc;&euro;&permil;I&acirc;&euro;&permil;X (NFIX), coordinates with the cis-regulatory elements at the SMARCD3 locus to form a chromatin hub to control SMARCD3 expression in the developing cerebellum and in metastatic MB. Increased SMARCD3 expression activates Reelin-DAB1-mediated Src kinase signalling, which results in a MB response to Src inhibition. These data deepen our understanding of how neurodevelopmental programming influences disease progression and provide a potential therapeutic option for patients with MB.</p>',
			'date' => '2023-02-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36849558',
			'doi' => '10.1038/s41556-023-01093-0',
			'modified' => '2023-03-14 09:41:24',
			'created' => '2023-03-02 17:27:08',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '4660',
			'name' => 'EBF1 is continuously required for stabilizing local chromatinaccessibility in pro-B cells.',
			'authors' => 'Zolotarev  Nikolay et al.',
			'description' => '<p>The establishment of de novo chromatin accessibility in lymphoid progenitors requires the "pioneering" function of transcription factor (TF) early B cell factor 1 (EBF1), which binds to na&iuml;ve chromatin and induces accessibility by recruiting the BRG1 chromatin remodeler subunit. However, it remains unclear whether the function of EBF1 is continuously required for stabilizing local chromatin accessibility. To this end, we replaced EBF1 by EBF1-FKBP in pro-B cells, allowing the rapid degradation by adding the degradation TAG13 (dTAG13) dimerizer. EBF1 degradation results in a loss of genome-wide EBF1 occupancy and EBF1-targeted BRG1 binding. Chromatin accessibility was rapidly diminished at EBF1-binding sites with a preference for sites whose occupancy requires the pioneering activity of the C-terminal domain of EBF1. Diminished chromatin accessibility correlated with altered gene expression. Thus, continuous activity of EBF1 is required for the stable maintenance of the transcriptional and epigenetic state of pro-B cells.</p>',
			'date' => '2022-11-01',
			'pmid' => 'https://doi.org/10.1073%2Fpnas',
			'doi' => '10.1073/pnas.2210595119',
			'modified' => '2023-03-07 09:07:41',
			'created' => '2023-02-21 09:59:46',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '2118',
			'name' => 'Tagmentation Buffer (2x) SDS GB en',
			'language' => 'en',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-GB-en-GHS_1_0.pdf',
			'countries' => 'GB',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '2120',
			'name' => 'Tagmentation Buffer (2x) SDS US en',
			'language' => 'en',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-US-en-GHS_1_0.pdf',
			'countries' => 'US',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '2116',
			'name' => 'Tagmentation Buffer (2x) SDS ES es',
			'language' => 'es',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-ES-es-GHS_1_0.pdf',
			'countries' => 'ES',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '2119',
			'name' => 'Tagmentation Buffer (2x) SDS JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-JP-ja-GHS_2_0.pdf',
			'countries' => 'JP',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '2115',
			'name' => 'Tagmentation Buffer (2x) SDS DE de',
			'language' => 'de',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-DE-de-GHS_1_0.pdf',
			'countries' => 'DE',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '2113',
			'name' => 'Tagmentation Buffer (2x) SDS BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-BE-fr-GHS_1_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '2114',
			'name' => 'Tagmentation Buffer (2x) SDS BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-BE-nl-GHS_1_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '2117',
			'name' => 'Tagmentation Buffer (2x) SDS FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-FR-fr-GHS_1_0.pdf',
			'countries' => 'FR',
			'modified' => '2021-10-12 14:27:13',
			'created' => '2021-10-12 14:27:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/tagmentation-buffer-2x'
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
	(int) 0 => array(
		'id' => '3162',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>
</div>
<div class="small-12 medium-4 large-4 columns"><center>
<script>// <![CDATA[
 var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } 
// ]]></script>
</center></div>
</div>
<p></p>',
		'label1' => 'Examples of use',
		'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label3' => '',
		'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'format' => '300 µl ',
		'catalog_number' => 'C01019043',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-29421',
		'type' => 'FRE',
		'search_order' => '',
		'price_EUR' => '90',
		'price_USD' => '100',
		'price_GBP' => '105',
		'price_JPY' => '14100',
		'price_CNY' => '',
		'price_AUD' => '250',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x',
		'meta_title' => 'Tagmentation Buffer 2x',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2023-06-20 18:19:03',
		'created' => '2020-12-08 16:00:14'
	),
	(int) 1 => array(
		'id' => '3163',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
		'label1' => 'Examples of use',
		'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label3' => '',
		'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'format' => '100 μl',
		'catalog_number' => 'C01019043-100',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-D001-001161',
		'type' => 'FRE',
		'search_order' => '',
		'price_EUR' => '50',
		'price_USD' => '50',
		'price_GBP' => '55',
		'price_JPY' => '7830',
		'price_CNY' => '',
		'price_AUD' => '125',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x-100',
		'meta_title' => 'Tagmentation Buffer 2x 100',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2022-11-25 12:25:10',
		'created' => '2020-12-08 16:06:10',
		'ProductsGroup' => array(
			'id' => '371',
			'product_id' => '3163',
			'group_id' => '330'
		)
	),
	(int) 2 => array(
		'id' => '3217',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
		'label1' => 'Examples of use',
		'info1' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label3' => '',
		'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'format' => '1000 µl',
		'catalog_number' => 'C01019043-1000',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-178850',
		'type' => 'FRE',
		'search_order' => '',
		'price_EUR' => '225',
		'price_USD' => '255',
		'price_GBP' => '270',
		'price_JPY' => '35245',
		'price_CNY' => '',
		'price_AUD' => '638',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x-1000',
		'meta_title' => 'Tagmentation Buffer 2x',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2023-01-04 15:29:39',
		'created' => '2022-04-13 15:31:11',
		'ProductsGroup' => array(
			'id' => '405',
			'product_id' => '3217',
			'group_id' => '330'
		)
	),
	(int) 3 => array(
		'id' => '3229',
		'antibody_id' => null,
		'name' => 'Tagmentation Buffer (2x)',
		'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
		'label1' => 'Examples of use',
		'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '10000 µl',
		'catalog_number' => 'C01019043-10000',
		'old_catalog_number' => '',
		'sf_code' => 'C01019043-190331',
		'type' => 'FRE',
		'search_order' => '1400, GBP 1640, USD 1500, AUD 3750, JPY ¥244,000',
		'price_EUR' => '1475',
		'price_USD' => '1585',
		'price_GBP' => '1730',
		'price_JPY' => '231060',
		'price_CNY' => '/',
		'price_AUD' => '3962',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'tagmentation-buffer-2x-10000',
		'meta_title' => 'Tagmentation Buffer 2x',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2022-11-25 12:29:09',
		'created' => '2022-11-25 11:26:45',
		'ProductsGroup' => array(
			'id' => '412',
			'product_id' => '3229',
			'group_id' => '330'
		)
	)
)
$pro = array(
	'id' => '3229',
	'antibody_id' => null,
	'name' => 'Tagmentation Buffer (2x)',
	'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
	'label1' => 'Examples of use',
	'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '10000 µl',
	'catalog_number' => 'C01019043-10000',
	'old_catalog_number' => '',
	'sf_code' => 'C01019043-190331',
	'type' => 'FRE',
	'search_order' => '1400, GBP 1640, USD 1500, AUD 3750, JPY ¥244,000',
	'price_EUR' => '1475',
	'price_USD' => '1585',
	'price_GBP' => '1730',
	'price_JPY' => '231060',
	'price_CNY' => '/',
	'price_AUD' => '3962',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '',
	'slug' => 'tagmentation-buffer-2x-10000',
	'meta_title' => 'Tagmentation Buffer 2x',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2022-11-25 12:29:09',
	'created' => '2022-11-25 11:26:45',
	'ProductsGroup' => array(
		'id' => '412',
		'product_id' => '3229',
		'group_id' => '330'
	)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
	'id' => '3229',
	'antibody_id' => null,
	'name' => 'Tagmentation Buffer (2x)',
	'description' => '<p>Diagenode <strong>Tagmentation Buffer (2x)</strong> is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase)</a> on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.</p>',
	'label1' => 'Examples of use',
	'info1' => '<p><span style="text-decoration: underline;">ATAC-seq experiments: </span></p>
<ul style="list-style-type: circle;">
<li>After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:</li>
</ul>
<table style="width: 447px;">
<tbody>
<tr>
<td style="width: 326px;">Tagmentation Buffer (2x)</td>
<td style="width: 114px; padding-left: 30px;">25 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tagmentase loaded</td>
<td style="width: 114px; padding-left: 30px;">2.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;"><span>Digitonin 1%</span></td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Tween20 10%</td>
<td style="width: 114px; padding-left: 30px;">0.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">PBS</td>
<td style="width: 114px; padding-left: 30px;">16.5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclease-free water</td>
<td style="width: 114px; padding-left: 30px;">&nbsp;5 &micro;l</td>
</tr>
<tr>
<td style="width: 326px;">Nuclei pellet*</td>
<td style="width: 114px;"></td>
</tr>
</tbody>
</table>
<p><em>*&nbsp; The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.&nbsp;</em></p>
<ul style="list-style-type: circle;">
<li>The reaction is then incubated 30 minutes at 37&deg;C.</li>
<li>The tagmentation reaction can then be stopped by addition of 250 &micro;l of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).</li>
<li>The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.</li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '10000 µl',
	'catalog_number' => 'C01019043-10000',
	'old_catalog_number' => '',
	'sf_code' => 'C01019043-190331',
	'type' => 'FRE',
	'search_order' => '1400, GBP 1640, USD 1500, AUD 3750, JPY ¥244,000',
	'price_EUR' => '1475',
	'price_USD' => '1585',
	'price_GBP' => '1730',
	'price_JPY' => '231060',
	'price_CNY' => '/',
	'price_AUD' => '3962',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '',
	'slug' => 'tagmentation-buffer-2x-10000',
	'meta_title' => 'Tagmentation Buffer 2x',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2022-11-25 12:29:09',
	'created' => '2022-11-25 11:26:45',
	'ProductsGroup' => array(
		'id' => '412',
		'product_id' => '3229',
		'group_id' => '330'
	)
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '1115',
	'name' => 'Datasheet Tagmentation Buffer (2x) C01019043',
	'description' => '',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/kits/Datasheet_Tagmentation Buffer_2x_C01019043.pdf',
	'slug' => 'tagmentation-buffer-2x-datasheet',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2020-12-10 17:02:47',
	'created' => '2020-12-10 17:02:47',
	'ProductsDocument' => array(
		'id' => '3259',
		'product_id' => '3228',
		'document_id' => '1115'
	)
)
$sds = array(
	'id' => '2117',
	'name' => 'Tagmentation Buffer (2x) SDS FR fr',
	'language' => 'fr',
	'url' => 'files/SDS/Tagmentase/SDS-C01019043-Tagmentation_Buffer_2x_-FR-fr-GHS_1_0.pdf',
	'countries' => 'FR',
	'modified' => '2021-10-12 14:27:13',
	'created' => '2021-10-12 14:27:13',
	'ProductsSafetySheet' => array(
		'id' => '4772',
		'product_id' => '3228',
		'safety_sheet_id' => '2117'
	)
)
$publication = array(
	'id' => '4660',
	'name' => 'EBF1 is continuously required for stabilizing local chromatinaccessibility in pro-B cells.',
	'authors' => 'Zolotarev  Nikolay et al.',
	'description' => '<p>The establishment of de novo chromatin accessibility in lymphoid progenitors requires the "pioneering" function of transcription factor (TF) early B cell factor 1 (EBF1), which binds to na&iuml;ve chromatin and induces accessibility by recruiting the BRG1 chromatin remodeler subunit. However, it remains unclear whether the function of EBF1 is continuously required for stabilizing local chromatin accessibility. To this end, we replaced EBF1 by EBF1-FKBP in pro-B cells, allowing the rapid degradation by adding the degradation TAG13 (dTAG13) dimerizer. EBF1 degradation results in a loss of genome-wide EBF1 occupancy and EBF1-targeted BRG1 binding. Chromatin accessibility was rapidly diminished at EBF1-binding sites with a preference for sites whose occupancy requires the pioneering activity of the C-terminal domain of EBF1. Diminished chromatin accessibility correlated with altered gene expression. Thus, continuous activity of EBF1 is required for the stable maintenance of the transcriptional and epigenetic state of pro-B cells.</p>',
	'date' => '2022-11-01',
	'pmid' => 'https://doi.org/10.1073%2Fpnas',
	'doi' => '10.1073/pnas.2210595119',
	'modified' => '2023-03-07 09:07:41',
	'created' => '2023-02-21 09:59:46',
	'ProductsPublication' => array(
		'id' => '6556',
		'product_id' => '3228',
		'publication_id' => '4660'
	)
)
$externalLink = ' <a href="https://doi.org/10.1073%2Fpnas" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Contact Information

First name

Last name

Company

Phone number

City

Country

State

Email verification^*

Comment

活动

查看所有活动

Site map | Contact us | Conditions of sales | Conditions of purchase | Privacy policy

Tagmentation Buffer (2x)	25 µl
Tagmentase loaded	2.5 µl
Digitonin 1%	0.5 µl
Tween20 10%	0.5 µl
PBS	16.5 µl
Nuclease-free water	5 µl
Nuclei pellet*

Tagmentation Buffer (2x) SDS GB en	Download
Tagmentation Buffer (2x) SDS US en	Download
Tagmentation Buffer (2x) SDS ES es	Download
Tagmentation Buffer (2x) SDS JP ja	Download
Tagmentation Buffer (2x) SDS DE de	Download
Tagmentation Buffer (2x) SDS BE fr	Download
Tagmentation Buffer (2x) SDS BE nl	Download
Tagmentation Buffer (2x) SDS FR fr	Download