Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'cn',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003',
'product' => array(
'Product' => array(
'id' => '3213',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq ',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p><p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <u>ATAC-seq package for tissue</u>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <u>ATAC-seq package for tissue</u>.</p><p><strong>Features:</strong></p><ul><li>Starting amount: 25 mg – 100 mg per rxn</li><li>Validated on many mammalian tissues</li><li>Included in a complete ATAC-seq workflow: <u>ATAC-seq package for tissue</u></li></ul><p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>',
'label1' => 'Example of results',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => '',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => '',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '8 rxns',
'catalog_number' => 'C01080003',
'old_catalog_number' => '',
'sf_code' => 'C01080003-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '160',
'price_USD' => '160',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '400',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080003',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:32:11',
'created' => '2022-03-23 15:07:48',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(),
'Category' => array(),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/tissue-nuclei-extraction-ATAC-seq-C01080003'
)
$language = 'cn'
$meta_keywords = ''
$meta_description = 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. '
$meta_title = 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003'
$product = array(
'Product' => array(
'id' => '3213',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq ',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p><p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <u>ATAC-seq package for tissue</u>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <u>ATAC-seq package for tissue</u>.</p><p><strong>Features:</strong></p><ul><li>Starting amount: 25 mg – 100 mg per rxn</li><li>Validated on many mammalian tissues</li><li>Included in a complete ATAC-seq workflow: <u>ATAC-seq package for tissue</u></li></ul><p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>',
'label1' => 'Example of results',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => '',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '8 rxns',
'catalog_number' => 'C01080003',
'old_catalog_number' => '',
'sf_code' => 'C01080003-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '160',
'price_USD' => '160',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '400',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080003',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:32:11',
'created' => '2022-03-23 15:07:48',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(
(int) 0 => array(
'id' => '353',
'name' => 'Tissue Nuclei Extraction for ATA',
'product_id' => '3213',
'modified' => '2022-03-23 15:22:41',
'created' => '2022-03-23 15:22:41'
)
),
'Group' => array(
'Group' => array(
'id' => '353',
'name' => 'Tissue Nuclei Extraction for ATA',
'product_id' => '3213',
'modified' => '2022-03-23 15:22:41',
'created' => '2022-03-23 15:22:41'
),
'Master' => array(
'id' => '3213',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq ',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p>
<p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>.</p>
<p><strong>Features:</strong></p>
<ul>
<li>Starting amount: 25 mg – 100 mg per rxn</li>
<li>Validated on many mammalian tissues</li>
<li>Included in a complete ATAC-seq workflow: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a></li>
</ul>
<p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>
<p></p>
<p><small><img src="https://icons.iconarchive.com/icons/wikipedia/flags/256/EU-European-Union-Flag-icon.png" alt="" width="45" /> The project GENE-SWitCH leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 817998.<small></small></small></p>',
'label1' => 'Method overview',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => 'Additional solutions for Tissue Nuclei Extraction for ATAC-seq',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '8 rxns',
'catalog_number' => 'C01080003',
'old_catalog_number' => '',
'sf_code' => 'C01080003-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '160',
'price_USD' => '160',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '400',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080003',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:32:11',
'created' => '2022-03-23 15:07:48'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(),
'Category' => array(),
'Document' => array(
(int) 0 => array(
'id' => '37',
'name' => 'Chromatin Brochure',
'description' => '<p>Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has everything you need to make ChIP easy and convenient while ensuring consistent data between samples and experiments. As an expert in the field of epigenetics, Diagenode is committed to providing complete solutions from chromatin shearing reagents, shearing instruments such as the Bioruptor® (the gold standard for chromatin shearing), ChIP kits, the largest number of validated and trusted antibodies on the market, and the SX-8G IP-Star® Compact Automated System to achieve unparalleled productivity and reproducibility.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Chromatin_Immunoprecipitation_Brochure.pdf',
'slug' => 'chromatin-immunoprecipitation-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-03-24 12:34:11',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1139',
'name' => 'ATAC-seq Kit - Manual',
'description' => '<p class="p1">Gene expression is carefully regulated in the cells in order to manage a wide range of biological functions. The structure of chromatin is quite dynamic and contributes to this crucial regulatory process.</p>
<p class="p1">ATAC-seq, Assay for Transposase-Accessible Chromatin, followed by nextgeneration sequencing, is a key technology to easily identify the “open” regions of the chromatin, which are usually associated with permissive gene expression. Indeed, the nuclei of the samples are incubated with a transposase, and only the genomic regions associated with open chromatin will be accessible to this transposase. During the process those regions will be cut and sequencing adaptors will be added, allowing their sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only., giving a map of the chromatin status in the whole genome of the sample.</p>
<p class="p1">The Diagenode’s ATAC-seq kit is based on a highly validated protocol, used for years in our Epigenomics Profiling Services offer and takes advantage of many successful Diagenode’s tools, such as the loaded Tagmentase (Tn5 transposase), the MicroChIP DiaPure Columns and the Primer indexes for tagmented libraries kits.</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/atacseq-kit-manual.pdf',
'slug' => 'atacseq-kit-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2021-06-25 09:51:59',
'created' => '2021-06-25 09:51:59',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '2432',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS US en',
'language' => 'en',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-US-en-1_0.pdf',
'countries' => 'US',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '2430',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS GB en',
'language' => 'en',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-GB-en-1_0.pdf',
'countries' => 'GB',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '2428',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS ES es',
'language' => 'es',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-ES-es-1_0.pdf',
'countries' => 'ES',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '2427',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS DE de',
'language' => 'de',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-DE-de-1_0.pdf',
'countries' => 'DE',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '2431',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-JP-ja-1_0.pdf',
'countries' => 'JP',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '2426',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-BE-nl-1_0.pdf',
'countries' => 'BE',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '2425',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-BE-fr-1_0.pdf',
'countries' => 'BE',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '2429',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-FR-fr-1_0.pdf',
'countries' => 'FR',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/tissue-nuclei-extraction-ATAC-seq-C01080003'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '3214',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p>
<p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>.</p>
<p>Features:</p>
<ul>
<li>Starting amount: 25 mg – 100 mg per rxn</li>
<li>Validated on many mammalian tissues</li>
<li>Included in a complete ATAC-seq workflow: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a></li>
</ul>
<p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>
<p></p>
<p><small><img src="https://icons.iconarchive.com/icons/wikipedia/flags/256/EU-European-Union-Flag-icon.png" alt="" width="45" /> The project GENE-SWitCH leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 817998.<small></small></small></p>',
'label1' => 'Method overview',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => 'Additional solutions for Tissue Nuclei Extraction for ATAC-seq',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '24 rxns',
'catalog_number' => 'C01080004',
'old_catalog_number' => '',
'sf_code' => 'C01080004-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '355',
'price_USD' => '355',
'price_GBP' => '305',
'price_JPY' => '55610',
'price_CNY' => '',
'price_AUD' => '888',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080004',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080004 ',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:31:40',
'created' => '2022-03-23 15:18:21',
'ProductsGroup' => array(
'id' => '403',
'product_id' => '3214',
'group_id' => '353'
)
)
)
$pro = array(
'id' => '3214',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p>
<p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>.</p>
<p>Features:</p>
<ul>
<li>Starting amount: 25 mg – 100 mg per rxn</li>
<li>Validated on many mammalian tissues</li>
<li>Included in a complete ATAC-seq workflow: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a></li>
</ul>
<p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>
<p></p>
<p><small><img src="https://icons.iconarchive.com/icons/wikipedia/flags/256/EU-European-Union-Flag-icon.png" alt="" width="45" /> The project GENE-SWitCH leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 817998.<small></small></small></p>',
'label1' => 'Method overview',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => 'Additional solutions for Tissue Nuclei Extraction for ATAC-seq',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '24 rxns',
'catalog_number' => 'C01080004',
'old_catalog_number' => '',
'sf_code' => 'C01080004-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '355',
'price_USD' => '355',
'price_GBP' => '305',
'price_JPY' => '55610',
'price_CNY' => '',
'price_AUD' => '888',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080004',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080004 ',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:31:40',
'created' => '2022-03-23 15:18:21',
'ProductsGroup' => array(
'id' => '403',
'product_id' => '3214',
'group_id' => '353'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '3214',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p>
<p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>.</p>
<p>Features:</p>
<ul>
<li>Starting amount: 25 mg – 100 mg per rxn</li>
<li>Validated on many mammalian tissues</li>
<li>Included in a complete ATAC-seq workflow: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a></li>
</ul>
<p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>
<p></p>
<p><small><img src="https://icons.iconarchive.com/icons/wikipedia/flags/256/EU-European-Union-Flag-icon.png" alt="" width="45" /> The project GENE-SWitCH leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 817998.<small></small></small></p>',
'label1' => 'Method overview',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => 'Additional solutions for Tissue Nuclei Extraction for ATAC-seq',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '24 rxns',
'catalog_number' => 'C01080004',
'old_catalog_number' => '',
'sf_code' => 'C01080004-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '355',
'price_USD' => '355',
'price_GBP' => '305',
'price_JPY' => '55610',
'price_CNY' => '',
'price_AUD' => '888',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080004',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080004 ',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:31:40',
'created' => '2022-03-23 15:18:21',
'ProductsGroup' => array(
'id' => '403',
'product_id' => '3214',
'group_id' => '353'
)
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '1139',
'name' => 'ATAC-seq Kit - Manual',
'description' => '<p class="p1">Gene expression is carefully regulated in the cells in order to manage a wide range of biological functions. The structure of chromatin is quite dynamic and contributes to this crucial regulatory process.</p>
<p class="p1">ATAC-seq, Assay for Transposase-Accessible Chromatin, followed by nextgeneration sequencing, is a key technology to easily identify the “open” regions of the chromatin, which are usually associated with permissive gene expression. Indeed, the nuclei of the samples are incubated with a transposase, and only the genomic regions associated with open chromatin will be accessible to this transposase. During the process those regions will be cut and sequencing adaptors will be added, allowing their sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only., giving a map of the chromatin status in the whole genome of the sample.</p>
<p class="p1">The Diagenode’s ATAC-seq kit is based on a highly validated protocol, used for years in our Epigenomics Profiling Services offer and takes advantage of many successful Diagenode’s tools, such as the loaded Tagmentase (Tn5 transposase), the MicroChIP DiaPure Columns and the Primer indexes for tagmented libraries kits.</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/atacseq-kit-manual.pdf',
'slug' => 'atacseq-kit-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2021-06-25 09:51:59',
'created' => '2021-06-25 09:51:59',
'ProductsDocument' => array(
'id' => '3205',
'product_id' => '3213',
'document_id' => '1139'
)
)
$sds = array(
'id' => '2429',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-FR-fr-1_0.pdf',
'countries' => 'FR',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
'id' => '4129',
'product_id' => '3213',
'safety_sheet_id' => '2429'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'cn',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003',
'product' => array(
'Product' => array(
'id' => '3213',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq ',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p><p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <u>ATAC-seq package for tissue</u>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <u>ATAC-seq package for tissue</u>.</p><p><strong>Features:</strong></p><ul><li>Starting amount: 25 mg – 100 mg per rxn</li><li>Validated on many mammalian tissues</li><li>Included in a complete ATAC-seq workflow: <u>ATAC-seq package for tissue</u></li></ul><p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>',
'label1' => 'Example of results',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => '',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => '',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '8 rxns',
'catalog_number' => 'C01080003',
'old_catalog_number' => '',
'sf_code' => 'C01080003-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '160',
'price_USD' => '160',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '400',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080003',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:32:11',
'created' => '2022-03-23 15:07:48',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(),
'Category' => array(),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/tissue-nuclei-extraction-ATAC-seq-C01080003'
)
$language = 'cn'
$meta_keywords = ''
$meta_description = 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. '
$meta_title = 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003'
$product = array(
'Product' => array(
'id' => '3213',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq ',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p><p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <u>ATAC-seq package for tissue</u>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <u>ATAC-seq package for tissue</u>.</p><p><strong>Features:</strong></p><ul><li>Starting amount: 25 mg – 100 mg per rxn</li><li>Validated on many mammalian tissues</li><li>Included in a complete ATAC-seq workflow: <u>ATAC-seq package for tissue</u></li></ul><p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>',
'label1' => 'Example of results',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => '',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '8 rxns',
'catalog_number' => 'C01080003',
'old_catalog_number' => '',
'sf_code' => 'C01080003-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '160',
'price_USD' => '160',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '400',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080003',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:32:11',
'created' => '2022-03-23 15:07:48',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(
(int) 0 => array(
'id' => '353',
'name' => 'Tissue Nuclei Extraction for ATA',
'product_id' => '3213',
'modified' => '2022-03-23 15:22:41',
'created' => '2022-03-23 15:22:41'
)
),
'Group' => array(
'Group' => array(
'id' => '353',
'name' => 'Tissue Nuclei Extraction for ATA',
'product_id' => '3213',
'modified' => '2022-03-23 15:22:41',
'created' => '2022-03-23 15:22:41'
),
'Master' => array(
'id' => '3213',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq ',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p>
<p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>.</p>
<p><strong>Features:</strong></p>
<ul>
<li>Starting amount: 25 mg – 100 mg per rxn</li>
<li>Validated on many mammalian tissues</li>
<li>Included in a complete ATAC-seq workflow: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a></li>
</ul>
<p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>
<p></p>
<p><small><img src="https://icons.iconarchive.com/icons/wikipedia/flags/256/EU-European-Union-Flag-icon.png" alt="" width="45" /> The project GENE-SWitCH leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 817998.<small></small></small></p>',
'label1' => 'Method overview',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => 'Additional solutions for Tissue Nuclei Extraction for ATAC-seq',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '8 rxns',
'catalog_number' => 'C01080003',
'old_catalog_number' => '',
'sf_code' => 'C01080003-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '160',
'price_USD' => '160',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '400',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080003',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:32:11',
'created' => '2022-03-23 15:07:48'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(),
'Category' => array(),
'Document' => array(
(int) 0 => array(
'id' => '37',
'name' => 'Chromatin Brochure',
'description' => '<p>Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has everything you need to make ChIP easy and convenient while ensuring consistent data between samples and experiments. As an expert in the field of epigenetics, Diagenode is committed to providing complete solutions from chromatin shearing reagents, shearing instruments such as the Bioruptor® (the gold standard for chromatin shearing), ChIP kits, the largest number of validated and trusted antibodies on the market, and the SX-8G IP-Star® Compact Automated System to achieve unparalleled productivity and reproducibility.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Chromatin_Immunoprecipitation_Brochure.pdf',
'slug' => 'chromatin-immunoprecipitation-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-03-24 12:34:11',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1139',
'name' => 'ATAC-seq Kit - Manual',
'description' => '<p class="p1">Gene expression is carefully regulated in the cells in order to manage a wide range of biological functions. The structure of chromatin is quite dynamic and contributes to this crucial regulatory process.</p>
<p class="p1">ATAC-seq, Assay for Transposase-Accessible Chromatin, followed by nextgeneration sequencing, is a key technology to easily identify the “open” regions of the chromatin, which are usually associated with permissive gene expression. Indeed, the nuclei of the samples are incubated with a transposase, and only the genomic regions associated with open chromatin will be accessible to this transposase. During the process those regions will be cut and sequencing adaptors will be added, allowing their sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only., giving a map of the chromatin status in the whole genome of the sample.</p>
<p class="p1">The Diagenode’s ATAC-seq kit is based on a highly validated protocol, used for years in our Epigenomics Profiling Services offer and takes advantage of many successful Diagenode’s tools, such as the loaded Tagmentase (Tn5 transposase), the MicroChIP DiaPure Columns and the Primer indexes for tagmented libraries kits.</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/atacseq-kit-manual.pdf',
'slug' => 'atacseq-kit-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2021-06-25 09:51:59',
'created' => '2021-06-25 09:51:59',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '2432',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS US en',
'language' => 'en',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-US-en-1_0.pdf',
'countries' => 'US',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '2430',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS GB en',
'language' => 'en',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-GB-en-1_0.pdf',
'countries' => 'GB',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '2428',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS ES es',
'language' => 'es',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-ES-es-1_0.pdf',
'countries' => 'ES',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '2427',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS DE de',
'language' => 'de',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-DE-de-1_0.pdf',
'countries' => 'DE',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '2431',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-JP-ja-1_0.pdf',
'countries' => 'JP',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '2426',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-BE-nl-1_0.pdf',
'countries' => 'BE',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '2425',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-BE-fr-1_0.pdf',
'countries' => 'BE',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '2429',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-FR-fr-1_0.pdf',
'countries' => 'FR',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/tissue-nuclei-extraction-ATAC-seq-C01080003'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '3214',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p>
<p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>.</p>
<p>Features:</p>
<ul>
<li>Starting amount: 25 mg – 100 mg per rxn</li>
<li>Validated on many mammalian tissues</li>
<li>Included in a complete ATAC-seq workflow: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a></li>
</ul>
<p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>
<p></p>
<p><small><img src="https://icons.iconarchive.com/icons/wikipedia/flags/256/EU-European-Union-Flag-icon.png" alt="" width="45" /> The project GENE-SWitCH leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 817998.<small></small></small></p>',
'label1' => 'Method overview',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => 'Additional solutions for Tissue Nuclei Extraction for ATAC-seq',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '24 rxns',
'catalog_number' => 'C01080004',
'old_catalog_number' => '',
'sf_code' => 'C01080004-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '355',
'price_USD' => '355',
'price_GBP' => '305',
'price_JPY' => '55610',
'price_CNY' => '',
'price_AUD' => '888',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080004',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080004 ',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:31:40',
'created' => '2022-03-23 15:18:21',
'ProductsGroup' => array(
'id' => '403',
'product_id' => '3214',
'group_id' => '353'
)
)
)
$pro = array(
'id' => '3214',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p>
<p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>.</p>
<p>Features:</p>
<ul>
<li>Starting amount: 25 mg – 100 mg per rxn</li>
<li>Validated on many mammalian tissues</li>
<li>Included in a complete ATAC-seq workflow: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a></li>
</ul>
<p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>
<p></p>
<p><small><img src="https://icons.iconarchive.com/icons/wikipedia/flags/256/EU-European-Union-Flag-icon.png" alt="" width="45" /> The project GENE-SWitCH leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 817998.<small></small></small></p>',
'label1' => 'Method overview',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => 'Additional solutions for Tissue Nuclei Extraction for ATAC-seq',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '24 rxns',
'catalog_number' => 'C01080004',
'old_catalog_number' => '',
'sf_code' => 'C01080004-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '355',
'price_USD' => '355',
'price_GBP' => '305',
'price_JPY' => '55610',
'price_CNY' => '',
'price_AUD' => '888',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080004',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080004 ',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:31:40',
'created' => '2022-03-23 15:18:21',
'ProductsGroup' => array(
'id' => '403',
'product_id' => '3214',
'group_id' => '353'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '3214',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p>
<p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>.</p>
<p>Features:</p>
<ul>
<li>Starting amount: 25 mg – 100 mg per rxn</li>
<li>Validated on many mammalian tissues</li>
<li>Included in a complete ATAC-seq workflow: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a></li>
</ul>
<p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>
<p></p>
<p><small><img src="https://icons.iconarchive.com/icons/wikipedia/flags/256/EU-European-Union-Flag-icon.png" alt="" width="45" /> The project GENE-SWitCH leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 817998.<small></small></small></p>',
'label1' => 'Method overview',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => 'Additional solutions for Tissue Nuclei Extraction for ATAC-seq',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '24 rxns',
'catalog_number' => 'C01080004',
'old_catalog_number' => '',
'sf_code' => 'C01080004-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '355',
'price_USD' => '355',
'price_GBP' => '305',
'price_JPY' => '55610',
'price_CNY' => '',
'price_AUD' => '888',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080004',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080004 ',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:31:40',
'created' => '2022-03-23 15:18:21',
'ProductsGroup' => array(
'id' => '403',
'product_id' => '3214',
'group_id' => '353'
)
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '1139',
'name' => 'ATAC-seq Kit - Manual',
'description' => '<p class="p1">Gene expression is carefully regulated in the cells in order to manage a wide range of biological functions. The structure of chromatin is quite dynamic and contributes to this crucial regulatory process.</p>
<p class="p1">ATAC-seq, Assay for Transposase-Accessible Chromatin, followed by nextgeneration sequencing, is a key technology to easily identify the “open” regions of the chromatin, which are usually associated with permissive gene expression. Indeed, the nuclei of the samples are incubated with a transposase, and only the genomic regions associated with open chromatin will be accessible to this transposase. During the process those regions will be cut and sequencing adaptors will be added, allowing their sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only., giving a map of the chromatin status in the whole genome of the sample.</p>
<p class="p1">The Diagenode’s ATAC-seq kit is based on a highly validated protocol, used for years in our Epigenomics Profiling Services offer and takes advantage of many successful Diagenode’s tools, such as the loaded Tagmentase (Tn5 transposase), the MicroChIP DiaPure Columns and the Primer indexes for tagmented libraries kits.</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/atacseq-kit-manual.pdf',
'slug' => 'atacseq-kit-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2021-06-25 09:51:59',
'created' => '2021-06-25 09:51:59',
'ProductsDocument' => array(
'id' => '3205',
'product_id' => '3213',
'document_id' => '1139'
)
)
$sds = array(
'id' => '2429',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-FR-fr-1_0.pdf',
'countries' => 'FR',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
'id' => '4129',
'product_id' => '3213',
'safety_sheet_id' => '2429'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'cn',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003',
'product' => array(
'Product' => array(
'id' => '3213',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq ',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p><p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <u>ATAC-seq package for tissue</u>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <u>ATAC-seq package for tissue</u>.</p><p><strong>Features:</strong></p><ul><li>Starting amount: 25 mg – 100 mg per rxn</li><li>Validated on many mammalian tissues</li><li>Included in a complete ATAC-seq workflow: <u>ATAC-seq package for tissue</u></li></ul><p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>',
'label1' => 'Example of results',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => '',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => '',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '8 rxns',
'catalog_number' => 'C01080003',
'old_catalog_number' => '',
'sf_code' => 'C01080003-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '160',
'price_USD' => '160',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '400',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080003',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:32:11',
'created' => '2022-03-23 15:07:48',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(),
'Category' => array(),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/tissue-nuclei-extraction-ATAC-seq-C01080003'
)
$language = 'cn'
$meta_keywords = ''
$meta_description = 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. '
$meta_title = 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003'
$product = array(
'Product' => array(
'id' => '3213',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq ',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p><p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <u>ATAC-seq package for tissue</u>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <u>ATAC-seq package for tissue</u>.</p><p><strong>Features:</strong></p><ul><li>Starting amount: 25 mg – 100 mg per rxn</li><li>Validated on many mammalian tissues</li><li>Included in a complete ATAC-seq workflow: <u>ATAC-seq package for tissue</u></li></ul><p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>',
'label1' => 'Example of results',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => '',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '8 rxns',
'catalog_number' => 'C01080003',
'old_catalog_number' => '',
'sf_code' => 'C01080003-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '160',
'price_USD' => '160',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '400',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080003',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:32:11',
'created' => '2022-03-23 15:07:48',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(
(int) 0 => array(
'id' => '353',
'name' => 'Tissue Nuclei Extraction for ATA',
'product_id' => '3213',
'modified' => '2022-03-23 15:22:41',
'created' => '2022-03-23 15:22:41'
)
),
'Group' => array(
'Group' => array(
'id' => '353',
'name' => 'Tissue Nuclei Extraction for ATA',
'product_id' => '3213',
'modified' => '2022-03-23 15:22:41',
'created' => '2022-03-23 15:22:41'
),
'Master' => array(
'id' => '3213',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq ',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p>
<p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>.</p>
<p><strong>Features:</strong></p>
<ul>
<li>Starting amount: 25 mg – 100 mg per rxn</li>
<li>Validated on many mammalian tissues</li>
<li>Included in a complete ATAC-seq workflow: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a></li>
</ul>
<p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>
<p></p>
<p><small><img src="https://icons.iconarchive.com/icons/wikipedia/flags/256/EU-European-Union-Flag-icon.png" alt="" width="45" /> The project GENE-SWitCH leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 817998.<small></small></small></p>',
'label1' => 'Method overview',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => 'Additional solutions for Tissue Nuclei Extraction for ATAC-seq',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '8 rxns',
'catalog_number' => 'C01080003',
'old_catalog_number' => '',
'sf_code' => 'C01080003-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '160',
'price_USD' => '160',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '400',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080003',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:32:11',
'created' => '2022-03-23 15:07:48'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(),
'Category' => array(),
'Document' => array(
(int) 0 => array(
'id' => '37',
'name' => 'Chromatin Brochure',
'description' => '<p>Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has everything you need to make ChIP easy and convenient while ensuring consistent data between samples and experiments. As an expert in the field of epigenetics, Diagenode is committed to providing complete solutions from chromatin shearing reagents, shearing instruments such as the Bioruptor® (the gold standard for chromatin shearing), ChIP kits, the largest number of validated and trusted antibodies on the market, and the SX-8G IP-Star® Compact Automated System to achieve unparalleled productivity and reproducibility.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Chromatin_Immunoprecipitation_Brochure.pdf',
'slug' => 'chromatin-immunoprecipitation-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-03-24 12:34:11',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1139',
'name' => 'ATAC-seq Kit - Manual',
'description' => '<p class="p1">Gene expression is carefully regulated in the cells in order to manage a wide range of biological functions. The structure of chromatin is quite dynamic and contributes to this crucial regulatory process.</p>
<p class="p1">ATAC-seq, Assay for Transposase-Accessible Chromatin, followed by nextgeneration sequencing, is a key technology to easily identify the “open” regions of the chromatin, which are usually associated with permissive gene expression. Indeed, the nuclei of the samples are incubated with a transposase, and only the genomic regions associated with open chromatin will be accessible to this transposase. During the process those regions will be cut and sequencing adaptors will be added, allowing their sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only., giving a map of the chromatin status in the whole genome of the sample.</p>
<p class="p1">The Diagenode’s ATAC-seq kit is based on a highly validated protocol, used for years in our Epigenomics Profiling Services offer and takes advantage of many successful Diagenode’s tools, such as the loaded Tagmentase (Tn5 transposase), the MicroChIP DiaPure Columns and the Primer indexes for tagmented libraries kits.</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/atacseq-kit-manual.pdf',
'slug' => 'atacseq-kit-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2021-06-25 09:51:59',
'created' => '2021-06-25 09:51:59',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '2432',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS US en',
'language' => 'en',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-US-en-1_0.pdf',
'countries' => 'US',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '2430',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS GB en',
'language' => 'en',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-GB-en-1_0.pdf',
'countries' => 'GB',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '2428',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS ES es',
'language' => 'es',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-ES-es-1_0.pdf',
'countries' => 'ES',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '2427',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS DE de',
'language' => 'de',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-DE-de-1_0.pdf',
'countries' => 'DE',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '2431',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-JP-ja-1_0.pdf',
'countries' => 'JP',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '2426',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-BE-nl-1_0.pdf',
'countries' => 'BE',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '2425',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-BE-fr-1_0.pdf',
'countries' => 'BE',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '2429',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-FR-fr-1_0.pdf',
'countries' => 'FR',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/tissue-nuclei-extraction-ATAC-seq-C01080003'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '3214',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p>
<p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>.</p>
<p>Features:</p>
<ul>
<li>Starting amount: 25 mg – 100 mg per rxn</li>
<li>Validated on many mammalian tissues</li>
<li>Included in a complete ATAC-seq workflow: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a></li>
</ul>
<p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>
<p></p>
<p><small><img src="https://icons.iconarchive.com/icons/wikipedia/flags/256/EU-European-Union-Flag-icon.png" alt="" width="45" /> The project GENE-SWitCH leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 817998.<small></small></small></p>',
'label1' => 'Method overview',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => 'Additional solutions for Tissue Nuclei Extraction for ATAC-seq',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '24 rxns',
'catalog_number' => 'C01080004',
'old_catalog_number' => '',
'sf_code' => 'C01080004-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '355',
'price_USD' => '355',
'price_GBP' => '305',
'price_JPY' => '55610',
'price_CNY' => '',
'price_AUD' => '888',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080004',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080004 ',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:31:40',
'created' => '2022-03-23 15:18:21',
'ProductsGroup' => array(
'id' => '403',
'product_id' => '3214',
'group_id' => '353'
)
)
)
$pro = array(
'id' => '3214',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p>
<p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>.</p>
<p>Features:</p>
<ul>
<li>Starting amount: 25 mg – 100 mg per rxn</li>
<li>Validated on many mammalian tissues</li>
<li>Included in a complete ATAC-seq workflow: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a></li>
</ul>
<p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>
<p></p>
<p><small><img src="https://icons.iconarchive.com/icons/wikipedia/flags/256/EU-European-Union-Flag-icon.png" alt="" width="45" /> The project GENE-SWitCH leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 817998.<small></small></small></p>',
'label1' => 'Method overview',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => 'Additional solutions for Tissue Nuclei Extraction for ATAC-seq',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '24 rxns',
'catalog_number' => 'C01080004',
'old_catalog_number' => '',
'sf_code' => 'C01080004-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '355',
'price_USD' => '355',
'price_GBP' => '305',
'price_JPY' => '55610',
'price_CNY' => '',
'price_AUD' => '888',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080004',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080004 ',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:31:40',
'created' => '2022-03-23 15:18:21',
'ProductsGroup' => array(
'id' => '403',
'product_id' => '3214',
'group_id' => '353'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '3214',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p>
<p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>.</p>
<p>Features:</p>
<ul>
<li>Starting amount: 25 mg – 100 mg per rxn</li>
<li>Validated on many mammalian tissues</li>
<li>Included in a complete ATAC-seq workflow: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a></li>
</ul>
<p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>
<p></p>
<p><small><img src="https://icons.iconarchive.com/icons/wikipedia/flags/256/EU-European-Union-Flag-icon.png" alt="" width="45" /> The project GENE-SWitCH leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 817998.<small></small></small></p>',
'label1' => 'Method overview',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => 'Additional solutions for Tissue Nuclei Extraction for ATAC-seq',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '24 rxns',
'catalog_number' => 'C01080004',
'old_catalog_number' => '',
'sf_code' => 'C01080004-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '355',
'price_USD' => '355',
'price_GBP' => '305',
'price_JPY' => '55610',
'price_CNY' => '',
'price_AUD' => '888',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080004',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080004 ',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:31:40',
'created' => '2022-03-23 15:18:21',
'ProductsGroup' => array(
'id' => '403',
'product_id' => '3214',
'group_id' => '353'
)
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '1139',
'name' => 'ATAC-seq Kit - Manual',
'description' => '<p class="p1">Gene expression is carefully regulated in the cells in order to manage a wide range of biological functions. The structure of chromatin is quite dynamic and contributes to this crucial regulatory process.</p>
<p class="p1">ATAC-seq, Assay for Transposase-Accessible Chromatin, followed by nextgeneration sequencing, is a key technology to easily identify the “open” regions of the chromatin, which are usually associated with permissive gene expression. Indeed, the nuclei of the samples are incubated with a transposase, and only the genomic regions associated with open chromatin will be accessible to this transposase. During the process those regions will be cut and sequencing adaptors will be added, allowing their sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only., giving a map of the chromatin status in the whole genome of the sample.</p>
<p class="p1">The Diagenode’s ATAC-seq kit is based on a highly validated protocol, used for years in our Epigenomics Profiling Services offer and takes advantage of many successful Diagenode’s tools, such as the loaded Tagmentase (Tn5 transposase), the MicroChIP DiaPure Columns and the Primer indexes for tagmented libraries kits.</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/atacseq-kit-manual.pdf',
'slug' => 'atacseq-kit-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2021-06-25 09:51:59',
'created' => '2021-06-25 09:51:59',
'ProductsDocument' => array(
'id' => '3205',
'product_id' => '3213',
'document_id' => '1139'
)
)
$sds = array(
'id' => '2429',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-FR-fr-1_0.pdf',
'countries' => 'FR',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
'id' => '4129',
'product_id' => '3213',
'safety_sheet_id' => '2429'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'cn',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003',
'product' => array(
'Product' => array(
'id' => '3213',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq ',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p><p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <u>ATAC-seq package for tissue</u>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <u>ATAC-seq package for tissue</u>.</p><p><strong>Features:</strong></p><ul><li>Starting amount: 25 mg – 100 mg per rxn</li><li>Validated on many mammalian tissues</li><li>Included in a complete ATAC-seq workflow: <u>ATAC-seq package for tissue</u></li></ul><p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>',
'label1' => 'Example of results',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => '',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => '',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '8 rxns',
'catalog_number' => 'C01080003',
'old_catalog_number' => '',
'sf_code' => 'C01080003-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '160',
'price_USD' => '160',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '400',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080003',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:32:11',
'created' => '2022-03-23 15:07:48',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(),
'Category' => array(),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/tissue-nuclei-extraction-ATAC-seq-C01080003'
)
$language = 'cn'
$meta_keywords = ''
$meta_description = 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. '
$meta_title = 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003'
$product = array(
'Product' => array(
'id' => '3213',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq ',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p><p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <u>ATAC-seq package for tissue</u>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <u>ATAC-seq package for tissue</u>.</p><p><strong>Features:</strong></p><ul><li>Starting amount: 25 mg – 100 mg per rxn</li><li>Validated on many mammalian tissues</li><li>Included in a complete ATAC-seq workflow: <u>ATAC-seq package for tissue</u></li></ul><p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>',
'label1' => 'Example of results',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => '',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '8 rxns',
'catalog_number' => 'C01080003',
'old_catalog_number' => '',
'sf_code' => 'C01080003-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '160',
'price_USD' => '160',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '400',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080003',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:32:11',
'created' => '2022-03-23 15:07:48',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(
(int) 0 => array(
'id' => '353',
'name' => 'Tissue Nuclei Extraction for ATA',
'product_id' => '3213',
'modified' => '2022-03-23 15:22:41',
'created' => '2022-03-23 15:22:41'
)
),
'Group' => array(
'Group' => array(
'id' => '353',
'name' => 'Tissue Nuclei Extraction for ATA',
'product_id' => '3213',
'modified' => '2022-03-23 15:22:41',
'created' => '2022-03-23 15:22:41'
),
'Master' => array(
'id' => '3213',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq ',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p>
<p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>.</p>
<p><strong>Features:</strong></p>
<ul>
<li>Starting amount: 25 mg – 100 mg per rxn</li>
<li>Validated on many mammalian tissues</li>
<li>Included in a complete ATAC-seq workflow: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a></li>
</ul>
<p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>
<p></p>
<p><small><img src="https://icons.iconarchive.com/icons/wikipedia/flags/256/EU-European-Union-Flag-icon.png" alt="" width="45" /> The project GENE-SWitCH leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 817998.<small></small></small></p>',
'label1' => 'Method overview',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => 'Additional solutions for Tissue Nuclei Extraction for ATAC-seq',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '8 rxns',
'catalog_number' => 'C01080003',
'old_catalog_number' => '',
'sf_code' => 'C01080003-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '160',
'price_USD' => '160',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '400',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080003',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080003',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:32:11',
'created' => '2022-03-23 15:07:48'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(),
'Category' => array(),
'Document' => array(
(int) 0 => array(
'id' => '37',
'name' => 'Chromatin Brochure',
'description' => '<p>Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has everything you need to make ChIP easy and convenient while ensuring consistent data between samples and experiments. As an expert in the field of epigenetics, Diagenode is committed to providing complete solutions from chromatin shearing reagents, shearing instruments such as the Bioruptor® (the gold standard for chromatin shearing), ChIP kits, the largest number of validated and trusted antibodies on the market, and the SX-8G IP-Star® Compact Automated System to achieve unparalleled productivity and reproducibility.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Chromatin_Immunoprecipitation_Brochure.pdf',
'slug' => 'chromatin-immunoprecipitation-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-03-24 12:34:11',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1139',
'name' => 'ATAC-seq Kit - Manual',
'description' => '<p class="p1">Gene expression is carefully regulated in the cells in order to manage a wide range of biological functions. The structure of chromatin is quite dynamic and contributes to this crucial regulatory process.</p>
<p class="p1">ATAC-seq, Assay for Transposase-Accessible Chromatin, followed by nextgeneration sequencing, is a key technology to easily identify the “open” regions of the chromatin, which are usually associated with permissive gene expression. Indeed, the nuclei of the samples are incubated with a transposase, and only the genomic regions associated with open chromatin will be accessible to this transposase. During the process those regions will be cut and sequencing adaptors will be added, allowing their sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only., giving a map of the chromatin status in the whole genome of the sample.</p>
<p class="p1">The Diagenode’s ATAC-seq kit is based on a highly validated protocol, used for years in our Epigenomics Profiling Services offer and takes advantage of many successful Diagenode’s tools, such as the loaded Tagmentase (Tn5 transposase), the MicroChIP DiaPure Columns and the Primer indexes for tagmented libraries kits.</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/atacseq-kit-manual.pdf',
'slug' => 'atacseq-kit-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2021-06-25 09:51:59',
'created' => '2021-06-25 09:51:59',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '2432',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS US en',
'language' => 'en',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-US-en-1_0.pdf',
'countries' => 'US',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '2430',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS GB en',
'language' => 'en',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-GB-en-1_0.pdf',
'countries' => 'GB',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '2428',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS ES es',
'language' => 'es',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-ES-es-1_0.pdf',
'countries' => 'ES',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '2427',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS DE de',
'language' => 'de',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-DE-de-1_0.pdf',
'countries' => 'DE',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '2431',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-JP-ja-1_0.pdf',
'countries' => 'JP',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '2426',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-BE-nl-1_0.pdf',
'countries' => 'BE',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '2425',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-BE-fr-1_0.pdf',
'countries' => 'BE',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '2429',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-FR-fr-1_0.pdf',
'countries' => 'FR',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/tissue-nuclei-extraction-ATAC-seq-C01080003'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '3214',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p>
<p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>.</p>
<p>Features:</p>
<ul>
<li>Starting amount: 25 mg – 100 mg per rxn</li>
<li>Validated on many mammalian tissues</li>
<li>Included in a complete ATAC-seq workflow: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a></li>
</ul>
<p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>
<p></p>
<p><small><img src="https://icons.iconarchive.com/icons/wikipedia/flags/256/EU-European-Union-Flag-icon.png" alt="" width="45" /> The project GENE-SWitCH leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 817998.<small></small></small></p>',
'label1' => 'Method overview',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => 'Additional solutions for Tissue Nuclei Extraction for ATAC-seq',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '24 rxns',
'catalog_number' => 'C01080004',
'old_catalog_number' => '',
'sf_code' => 'C01080004-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '355',
'price_USD' => '355',
'price_GBP' => '305',
'price_JPY' => '55610',
'price_CNY' => '',
'price_AUD' => '888',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080004',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080004 ',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:31:40',
'created' => '2022-03-23 15:18:21',
'ProductsGroup' => array(
'id' => '403',
'product_id' => '3214',
'group_id' => '353'
)
)
)
$pro = array(
'id' => '3214',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p>
<p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>.</p>
<p>Features:</p>
<ul>
<li>Starting amount: 25 mg – 100 mg per rxn</li>
<li>Validated on many mammalian tissues</li>
<li>Included in a complete ATAC-seq workflow: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a></li>
</ul>
<p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>
<p></p>
<p><small><img src="https://icons.iconarchive.com/icons/wikipedia/flags/256/EU-European-Union-Flag-icon.png" alt="" width="45" /> The project GENE-SWitCH leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 817998.<small></small></small></p>',
'label1' => 'Method overview',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => 'Additional solutions for Tissue Nuclei Extraction for ATAC-seq',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '24 rxns',
'catalog_number' => 'C01080004',
'old_catalog_number' => '',
'sf_code' => 'C01080004-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '355',
'price_USD' => '355',
'price_GBP' => '305',
'price_JPY' => '55610',
'price_CNY' => '',
'price_AUD' => '888',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080004',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080004 ',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:31:40',
'created' => '2022-03-23 15:18:21',
'ProductsGroup' => array(
'id' => '403',
'product_id' => '3214',
'group_id' => '353'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '3214',
'antibody_id' => null,
'name' => 'Tissue Nuclei Extraction for ATAC-seq',
'description' => '<p>Diagenode’s <b>Tissue </b><b>Nuclei</b><b> Extraction for ATAC-</b><b>seq</b><b> module</b> has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).</p>
<p>For a <b>complete ATAC-seq for tissue workflow</b> we highly recommend to use the <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a>.</p>
<p>Features:</p>
<ul>
<li>Starting amount: 25 mg – 100 mg per rxn</li>
<li>Validated on many mammalian tissues</li>
<li>Included in a complete ATAC-seq workflow: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080005" target="_blank" title="ATAC-seq package for tissue">ATAC-seq package for tissue</a></li>
</ul>
<p>Looking for ATAC-seq for cells? Please go to: <a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">ATAC-</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns">seq</a><a href="https://www.diagenode.com/en/p/atac-seq-kit-8rxns"> kit</a></p>
<p></p>
<p><small><img src="https://icons.iconarchive.com/icons/wikipedia/flags/256/EU-European-Union-Flag-icon.png" alt="" width="45" /> The project GENE-SWitCH leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 817998.<small></small></small></p>',
'label1' => 'Method overview',
'info1' => '<p><b>ATAC-seq</b>, <b>A</b>ssay for <b>T</b>ransposase-<b>A</b>ccessible <b>C</b>hromatin, followed by next generation sequencing, is a key technology to easily identify the <b>open regions of the chromatin.</b> The protocol consists of <b>3 steps</b>: <b>nuclei preparation</b>, <b>tagmentation</b> and <b>library amplification</b>. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq-tissue.png" alt="workflow" style="display: block; margin-left: auto; margin-right: auto;" width="600px" /></p>',
'label2' => 'Example of results',
'info2' => '<p>GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/table1-atacseq-results.png" width="400" /></center>
<p><small><strong>Table 1.</strong> List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.</small></p>
<p>A.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2a-atacseq-results.png" width="700" /></center>
<p>B.</p>
<center><img src="https://www.diagenode.com/img/product/kits/atacseq/fig2b-atacseq-results.png" width="700" /></center>
<p><small><strong>Figure 2.</strong> ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.</small></p>',
'label3' => 'Additional solutions for Tissue Nuclei Extraction for ATAC-seq',
'info3' => '<ul>
<li><a href="https://www.diagenode.com/en/p/protease-inhibitor-mix-100-ul">Protease Inhibitor Mix 200X</a></li>
</ul>',
'format' => '24 rxns',
'catalog_number' => 'C01080004',
'old_catalog_number' => '',
'sf_code' => 'C01080004-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '355',
'price_USD' => '355',
'price_GBP' => '305',
'price_JPY' => '55610',
'price_CNY' => '',
'price_AUD' => '888',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'tissue-nuclei-extraction-ATAC-seq-C01080004',
'meta_title' => 'Tissue Nuclei Extraction for ATAC-seq |Diagenode C01080004 ',
'meta_keywords' => '',
'meta_description' => 'Diagenode’s Tissue Nuclei Extraction for ATAC-seq provides a robust protocol for efficient nuclei extraction prior to ATAC-seq protocol. ',
'modified' => '2023-01-04 15:31:40',
'created' => '2022-03-23 15:18:21',
'ProductsGroup' => array(
'id' => '403',
'product_id' => '3214',
'group_id' => '353'
)
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '1139',
'name' => 'ATAC-seq Kit - Manual',
'description' => '<p class="p1">Gene expression is carefully regulated in the cells in order to manage a wide range of biological functions. The structure of chromatin is quite dynamic and contributes to this crucial regulatory process.</p>
<p class="p1">ATAC-seq, Assay for Transposase-Accessible Chromatin, followed by nextgeneration sequencing, is a key technology to easily identify the “open” regions of the chromatin, which are usually associated with permissive gene expression. Indeed, the nuclei of the samples are incubated with a transposase, and only the genomic regions associated with open chromatin will be accessible to this transposase. During the process those regions will be cut and sequencing adaptors will be added, allowing their sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only., giving a map of the chromatin status in the whole genome of the sample.</p>
<p class="p1">The Diagenode’s ATAC-seq kit is based on a highly validated protocol, used for years in our Epigenomics Profiling Services offer and takes advantage of many successful Diagenode’s tools, such as the loaded Tagmentase (Tn5 transposase), the MicroChIP DiaPure Columns and the Primer indexes for tagmented libraries kits.</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/atacseq-kit-manual.pdf',
'slug' => 'atacseq-kit-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2021-06-25 09:51:59',
'created' => '2021-06-25 09:51:59',
'ProductsDocument' => array(
'id' => '3205',
'product_id' => '3213',
'document_id' => '1139'
)
)
$sds = array(
'id' => '2429',
'name' => 'Tissue Nuclei Extraction for ATAC-seq SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/ATAC-seq/SDS-C01080003_C01080004-Tissue_Nuclei_Extraction_for_ATAC-seq-FR-fr-1_0.pdf',
'countries' => 'FR',
'modified' => '2022-08-30 10:30:59',
'created' => '2022-08-30 10:30:59',
'ProductsSafetySheet' => array(
'id' => '4129',
'product_id' => '3213',
'safety_sheet_id' => '2429'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
×
