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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against ZMYM3</strong><br />ChIP was performed using HepG2 cells, the Diagenode monoclonal antibody against ZMYM3 (Cat. No. C15200016) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers near the CAMK2N1 and SCML1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against ZMYM3</strong><br />ChIP was performed with 1 μg of the Diagenode antibody against ZMYM3 (Cat. No. C15200016) on sheared chromatin from 4 million HepG2 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 2 Mb region of chromosome 3 (figure 2A and B) and in two regions surrounding the SCML1 and CAMK2N1 positive control genes (figure 2C and D). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
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<p><small> <strong>Figure 3. Protein array analysis with the Diagenode antibody directed against ZMYM3</strong> <br />The specificity of the Diagenode antibody against ZMYM3 (Cat. No. C15200016) was demonstrated using the HuProt human protein microarray (CDI Laboratories), a protein array containing more than 19,000 human proteins. The antibody was used at a dilution of 1:100,000. Figure 4 shows the Z-score of the signal intensity (mean value of the duplicate spots on the array). The names of the proteins with 3 highest Z-scores are indicated at the bottom. This figure clearly shows the high specificity of the antibody for ZMYM3.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against ZMYM3</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against ZMYM3 (cat. No. C15200016) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the ZMYM3 antibody (middle) diluted 1:1.000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against ZMYM3</strong><br />ChIP was performed using HepG2 cells, the Diagenode monoclonal antibody against ZMYM3 (Cat. No. C15200016) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers near the CAMK2N1 and SCML1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against ZMYM3</strong><br />ChIP was performed with 1 μg of the Diagenode antibody against ZMYM3 (Cat. No. C15200016) on sheared chromatin from 4 million HepG2 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 2 Mb region of chromosome 3 (figure 2A and B) and in two regions surrounding the SCML1 and CAMK2N1 positive control genes (figure 2C and D). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
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<p><small> <strong>Figure 3. Protein array analysis with the Diagenode antibody directed against ZMYM3</strong> <br />The specificity of the Diagenode antibody against ZMYM3 (Cat. No. C15200016) was demonstrated using the HuProt human protein microarray (CDI Laboratories), a protein array containing more than 19,000 human proteins. The antibody was used at a dilution of 1:100,000. Figure 4 shows the Z-score of the signal intensity (mean value of the duplicate spots on the array). The names of the proteins with 3 highest Z-scores are indicated at the bottom. This figure clearly shows the high specificity of the antibody for ZMYM3.</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against ZMYM3</strong><br />ChIP was performed with 1 μg of the Diagenode antibody against ZMYM3 (Cat. No. C15200016) on sheared chromatin from 4 million HepG2 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 2 Mb region of chromosome 3 (figure 2A and B) and in two regions surrounding the SCML1 and CAMK2N1 positive control genes (figure 2C and D). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against ZMYM3</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against ZMYM3 (cat. No. C15200016) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the ZMYM3 antibody (middle) diluted 1:1.000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against ZMYM3</strong><br />ChIP was performed using HepG2 cells, the Diagenode monoclonal antibody against ZMYM3 (Cat. No. C15200016) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers near the CAMK2N1 and SCML1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200015-chipseq-A.jpg" alt="ZMYM3 Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200015-chipseq-B.jpg" alt="ZMYM3 Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200015-chipseq-C.jpg" alt="ZMYM3 Antibody ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200015-chipseq-D.jpg" alt="ZMYM3 Antibody validated in ChIP-seq" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against ZMYM3</strong><br />ChIP was performed with 1 μg of the Diagenode antibody against ZMYM3 (Cat. No. C15200016) on sheared chromatin from 4 million HepG2 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 2 Mb region of chromosome 3 (figure 2A and B) and in two regions surrounding the SCML1 and CAMK2N1 positive control genes (figure 2C and D). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200015-protein-array.jpg" alt="ZMYM3 Antibody validated in Protein array" /></p>
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<p><small> <strong>Figure 3. Protein array analysis with the Diagenode antibody directed against ZMYM3</strong> <br />The specificity of the Diagenode antibody against ZMYM3 (Cat. No. C15200016) was demonstrated using the HuProt human protein microarray (CDI Laboratories), a protein array containing more than 19,000 human proteins. The antibody was used at a dilution of 1:100,000. Figure 4 shows the Z-score of the signal intensity (mean value of the duplicate spots on the array). The names of the proteins with 3 highest Z-scores are indicated at the bottom. This figure clearly shows the high specificity of the antibody for ZMYM3.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against ZMYM3</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against ZMYM3 (cat. No. C15200016) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the ZMYM3 antibody (middle) diluted 1:1.000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<td>ChIP /ChIP-seq <sup>*</sup></td>
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<td>Fig 1, 2</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.</small></p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against ZMYM3</strong><br />ChIP was performed using HepG2 cells, the Diagenode monoclonal antibody against ZMYM3 (Cat. No. C15200016) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers near the CAMK2N1 and SCML1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200015-chipseq-B.jpg" alt="ZMYM3 Antibody for ChIP-seq" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against ZMYM3</strong><br />ChIP was performed with 1 μg of the Diagenode antibody against ZMYM3 (Cat. No. C15200016) on sheared chromatin from 4 million HepG2 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 2 Mb region of chromosome 3 (figure 2A and B) and in two regions surrounding the SCML1 and CAMK2N1 positive control genes (figure 2C and D). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
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<p><small> <strong>Figure 3. Protein array analysis with the Diagenode antibody directed against ZMYM3</strong> <br />The specificity of the Diagenode antibody against ZMYM3 (Cat. No. C15200016) was demonstrated using the HuProt human protein microarray (CDI Laboratories), a protein array containing more than 19,000 human proteins. The antibody was used at a dilution of 1:100,000. Figure 4 shows the Z-score of the signal intensity (mean value of the duplicate spots on the array). The names of the proteins with 3 highest Z-scores are indicated at the bottom. This figure clearly shows the high specificity of the antibody for ZMYM3.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against ZMYM3</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against ZMYM3 (cat. No. C15200016) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the ZMYM3 antibody (middle) diluted 1:1.000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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