D-Plex technology combines RNA tailing and template-switching
The D-Plex technology utilizes the innovative capture and amplification by tailing and switching, a
unique, ultrasensitive ligation-free method to generate RNA libraries for next generation sequencing.
This ingenious enzymatic technology offers key advantages compared to ligase-based approach:
- Low to ultra-low input capability with high reproducibility
- Higher library complexity providing novel and diverse transcript detection
- Optimal performance on challenging samples, such as liquid biopsies and FFPE samples
The D-Plex technology offers new RNA-seq solution, optimized and specifically formatted for both
Illumina’s
and MGI’s sequencing platforms.
D-Plex provides high transcript diversity
Thanks to the template-switching mechanism, D-Plex RNA-seq solution ensures a realistic representation
of the transcriptome of your sample of interest.
The D-Plex Small RNA-seq kit and the D-Plex Small RNA DNBSEQ kit efficiently incorporate a wide variety
of small RNA species, such as microRNAs (miRNAs), PIWI-interacting RNAs (piRNAs), small nucleolar RNAs
(snoRNAs), small nuclear RNAs (snRNAs) and tRNA-derived small RNAs, which represent new potential
classes of biomarkers to be explored as they have proved to be key components for cellular regulation in
some diseases.
D-Plex is highly suitable for low RNA inputs and challenging samples
The input requirements for D-Plex solutions are flexible and allow the user to perform the library
preparation within a wide range of RNA quantities going from 10 pg of small RNAs (< 200 nt) or
circulating RNAs up to 100 ng of total RNAs.
The D-Plex RNA-seq kits have been extensively validated for clinically relevant samples such as
liquid biopsies or FFPE samples. The D-Plex technology is exceedingly suitable for epigenetic
biomarker discovery.
