Deparaffinization - Procedure for FFPE nucleic acid extraction with the Bioruptor®

Deparafinization of FFPE samples is typically performed using a non-polar solvent, such as xylene, or a mineral oil-based method which can be time consuming and messy. Claire Josse and colleagues from the Human Genetic Laboratory at the GIGA - University of Liège have developed a new protocol combining the Bioruptor® Pico with the AllPrep FFPE DNA/RNA kit from Qiagen® to ef ciently prepare FFPE samples for Next-Generation Sequencing. The Bioruptor® Pico removes the parafin and rehydrates the tissue in just one solvent-free step followed by a mild crosslink reversal to preserve DNA and RNA integrity. The nucleic acids are then extracted with the AllPrep FFPE DNA/RNA kit and sheared using the Bioruptor® Pico to the desired length for sequencing library preparation.