PCR amplicons shearing using the Bioruptor Pico

With the Bioruptor® Pico and the former model Bioruptor® NGS it is possible to shear PCR amplicons of at least 500 bp in size. While PCR fragments bigger than 700 bp are sheared according to the standard protocol (detailed on next page), small PCR fragments from 500 - 700 bp are less susceptible to sonication.