Methylated DNA Immunoprecipitation Analysis of Mammalian Endogenous Retroviruses.

Rebollo R, Mager DL

Endogenous retroviruses are repetitive sequences found abundantly in mammalian genomes which are capable of modulating host gene expression. Nevertheless, most endogenous retrovirus copies are under tight epigenetic control via histone-repressive modifications and DNA methylation. Here we describe a common method used in our laboratory to detect, quantify, and compare mammalian endogenous retrovirus DNA methylation. More specifically we describe methylated DNA immunoprecipitation (MeDIP) followed by quantitative PCR.

Tags
Bioruptor
Antibody
5mC (C15200081)

Share this article
Tweet

Published
February, 2016

Source

Products used in this publication

C15200081-100

5-methylcytosine (5-mC) Antibody - clone 33D3
C15400001-15

Mouse IgG Antibodies - Chip

Methylated DNA Immunoprecipitation Analysis of Mammalian Endogenous Retroviruses.

Products used in this publication

5-methylcytosine (5-mC) Antibody - clone 33D3

Mouse IgG Antibodies - Chip

活动