High resolution methylation analysis of the HoxA5 regulatory region in different somatic tissues of laboratory mouse during development

Sinha P. et al.

Homeobox genes encode a group of DNA binding regulatory proteins whose key function occurs in the spatial-temporal organization of genome during embryonic development and differentiation. The role of these Hox genes during ontogenesis makes it an important model for research. HoxA5 is a member of Hox gene family playing a central role during axial body patterning and morphogenesis. DNA modification studies have shown that the function of Hox genes is partly governed by the methylation-mediated gene expression regulation. Therefore the study aimed to investigate the role of epigenetic events in regulation of tissue-specific expression pattern of HoxA5 gene during mammalian development. The methodology adopted were sodium bisulfite genomic DNA sequencing, quantitative real-time PCR and chromatin-immunoprecipitation (ChIP). Methylation profiling of HoxA5 gene promoter shows higher methylation in adult as compared to fetus in various somatic tissues of mouse being highest in adult spleen. However q-PCR results show higher expression during fetal stages being highest in fetal intestine followed by brain, liver and spleen. These results clearly indicate a strict correlation between DNA methylation and tissue-specific gene expression. The findings of chromatin-immunoprecipitation (ChIP) have also reinforced that epigenetic event like DNA methylation plays important role in the regulation of tissue specific expression of HoxA5.

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Histone ChIP kit

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Published
January, 2017

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Products used in this publication

C15200152

H3K4me3 Antibody - ChIP-seq Grade
C15200146

H3K9me3 monoclonal antibody
C15400001-15

Mouse IgG Antibodies - Chip
C17021045-50

Mouse GAPDH promoter primer pair
C17021042-50

Mouse TSH2B coding region primer pair

High resolution methylation analysis of the HoxA5 regulatory region in different somatic tissues of laboratory mouse during development

Products used in this publication

H3K4me3 Antibody - ChIP-seq Grade

H3K9me3 monoclonal antibody

Mouse IgG Antibodies - Chip

Mouse GAPDH promoter primer pair

Mouse TSH2B coding region primer pair

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