We present a genome assembly from an individual female Leucozona laternaria (the dark-saddled leucozona; Arthropoda; Insecta; Diptera; Syrphidae). The genome sequence is 614 megabases in span. Most of the assembly (99.69\%) is scaffolded into five chromosomal pseudomolecules including the assembled X sex chromosome. The mitochondrial genome has also been assembled and is 18.3 kilobases in length. Gene annotation of this assembly on Ensembl has identified 11,677 protein coding genes.