We present a genome assembly from an individual female Bombylius discolor (the dotted bee-fly; Arthropoda; Insecta; Diptera; Bombyliidae). The genome sequence is 280 megabases in span. Most of the assembly (99.93\%) is scaffolded into six chromosomal pseudomolecules, with the X sex chromosome assembled. The mitochondrial genome has also been assembled and is 16.7 kilobases in length. Genome annotation identified 10,411 protein-coding genes.