Background ‘Chambourcin’ is a French-American interspecific hybrid grape variety grown in the eastern and midwestern United States and used for making wine. Currently, there are few genomic resources available for hybrid grapevines like ‘Chambourcin’. Results We assembled the genome of ‘Chambourcin’ using PacBio HiFi long-read sequencing and Bionano optical map sequencing. We produced an assembly for ‘Chambourcin’ with 27 scaffolds with an N50 length of 23.3 Mb and an estimated BUSCO completeness of 98.2\%. 33,265 gene models were predicted, of which 81\% (26,886) were functionally annotated using Gene Ontology and KEGG pathway analysis. We identified 16,501 common orthologs between ‘Chambourcin’ gene models, V. vinifera ‘PN40024’ 12X.v2, VCOST.v3, V. riparia ‘Manitoba 37’ and V. riparia Gloire. A total of 1,589 plant transcription factors representing 58 different gene families were identified in ‘Chambourcin’. Finally, we identified 310,963 simple sequence repeats (SSRs), repeating units of 16 base pairs in length in the ‘Chambourcin’ genome assembly. Conclusions We present the genome assembly, genome annotation, protein sequences and coding sequences reported for ‘Chambourcin’. The ‘Chambourcin’ genome assembly provides a valuable resource for genome comparisons, functional genomic analysis, and genome-assisted breeding research.