The 20 μl microfluidic chips includes everything for best DNA shearing:
- 12ml syringe filled with the ultrasonic gel (1x)
- 20 μl microfluidic chips (10x)
- Precut cover tape (10x)
The 20 μl microfluidic chips were specifically designed for efficient DNA shearing (200bp - 1kb) with the One. They are simple to use and handle.
The 20 μl microfluidic chips includes everything for best DNA shearing:
Follow our guidelines https://www.diagenode.com/en/dna-shearing-guide and find the adequate length of sonication for your expected DNA size.
 How to properly cite this product in your workDiagenode strongly recommends using this: 20 μl Microfluidic Chips for the One (Diagenode Cat# C30150001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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The fully integrated cooling system and the all-new<sup> </sup>20 and 50 µl microfluidic chips have been enhanced to deliver the highest performance. </p> <p></p> </div> </div> <div class="row"> <div class="small-12 medium-12 large-12 columns text-left"> <ul> <li><span>Compact size</span></li> <li><span>Small volume DNA and Chromatin shearing for optimal sample prep</span></li> <li><span>Fully integrated system</span></li> </ul> </div> </div>', 'label1' => 'Highly reproducible DNA shearing ', 'info1' => '<p>The One provide the most consistent and ideal shearing (200 bp - 1 kb) to ensure subsequent high quality NGS data.</p> <p></p> <div class="row"> <div class="small-3 medium-3 large-3 columns img-center"> <p><small><strong>High and homogeneous shearing performance.</strong> Fragment Analyzer<sup>TM</sup> (Advanced Analytical Technologies, Inc.) profile showing the tight distribution for 8 independent DNA samples prepared with the One.</small></p> </div> <div class="small-9 medium-9 large-9 columns img-center"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-200bp-dna-shearing.jpg" alt="" height="422" width="942" /></div> </div>', 'label2' => 'Unbiased DNA shearing for Next-Generation Sequencing', 'info2' => '<p>The One is specifically designed to prepare high-quality sheared DNA prior to create unbiaised libraries with even distribution and representation of the genome. 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Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div> </div> <p></p> <div class="row"> <div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div> <div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div> <div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div> <div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. 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Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p> </div> </div> <form action="/en/quotes/quote?id=2766" id="Quote-2766" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="2766" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="d0U3c1YwZC84dStqcXJ5RU81OXVPdz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="bioruptor-one-sonication-device" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/> <div class="row collapse"> <h2>Contact Information</h2> <div class="small-3 large-2 columns"> <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Phone number</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">City</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-2766"> <option value="">-- select a country --</option> <option value="AF">Afghanistan</option> <option value="AX">Åland Islands</option> <option value="AL">Albania</option> <option value="DZ">Algeria</option> <option value="AS">American Samoa</option> <option value="AD">Andorra</option> <option value="AO">Angola</option> <option value="AI">Anguilla</option> <option value="AQ">Antarctica</option> <option value="AG">Antigua and Barbuda</option> <option value="AR">Argentina</option> <option value="AM">Armenia</option> <option value="AW">Aruba</option> <option value="AU">Australia</option> <option value="AT">Austria</option> <option value="AZ">Azerbaijan</option> <option value="BS">Bahamas</option> <option value="BH">Bahrain</option> <option value="BD">Bangladesh</option> <option value="BB">Barbados</option> <option value="BY">Belarus</option> <option value="BE">Belgium</option> <option value="BZ">Belize</option> <option value="BJ">Benin</option> <option value="BM">Bermuda</option> <option value="BT">Bhutan</option> <option value="BO">Bolivia</option> <option value="BQ">Bonaire, Sint Eustatius and Saba</option> <option value="BA">Bosnia and Herzegovina</option> <option value="BW">Botswana</option> <option value="BV">Bouvet Island</option> <option value="BR">Brazil</option> <option value="IO">British Indian Ocean Territory</option> <option value="BN">Brunei Darussalam</option> <option value="BG">Bulgaria</option> <option value="BF">Burkina Faso</option> <option value="BI">Burundi</option> <option value="KH">Cambodia</option> <option value="CM">Cameroon</option> <option value="CA">Canada</option> <option value="CV">Cape Verde</option> <option value="KY">Cayman Islands</option> <option value="CF">Central African Republic</option> <option value="TD">Chad</option> <option value="CL">Chile</option> <option value="CN">China</option> <option value="CX">Christmas Island</option> <option value="CC">Cocos (Keeling) Islands</option> <option value="CO">Colombia</option> <option value="KM">Comoros</option> <option value="CG">Congo</option> <option value="CD">Congo, The Democratic Republic of the</option> <option value="CK">Cook Islands</option> <option value="CR">Costa Rica</option> <option value="CI">Côte d'Ivoire</option> <option value="HR">Croatia</option> <option value="CU">Cuba</option> <option value="CW">Curaçao</option> <option value="CY">Cyprus</option> <option value="CZ">Czech Republic</option> <option value="DK">Denmark</option> <option value="DJ">Djibouti</option> <option value="DM">Dominica</option> <option value="DO">Dominican Republic</option> <option value="EC">Ecuador</option> <option value="EG">Egypt</option> <option value="SV">El Salvador</option> <option value="GQ">Equatorial Guinea</option> <option value="ER">Eritrea</option> <option value="EE">Estonia</option> <option value="ET">Ethiopia</option> <option value="FK">Falkland Islands (Malvinas)</option> <option value="FO">Faroe Islands</option> <option value="FJ">Fiji</option> <option value="FI">Finland</option> <option value="FR">France</option> <option value="GF">French Guiana</option> <option value="PF">French Polynesia</option> <option value="TF">French Southern Territories</option> <option value="GA">Gabon</option> <option value="GM">Gambia</option> <option value="GE">Georgia</option> <option value="DE">Germany</option> <option value="GH">Ghana</option> <option value="GI">Gibraltar</option> <option value="GR">Greece</option> <option value="GL">Greenland</option> <option value="GD">Grenada</option> <option value="GP">Guadeloupe</option> <option value="GU">Guam</option> <option value="GT">Guatemala</option> <option value="GG">Guernsey</option> <option value="GN">Guinea</option> <option value="GW">Guinea-Bissau</option> <option value="GY">Guyana</option> <option value="HT">Haiti</option> <option value="HM">Heard Island and McDonald Islands</option> <option value="VA">Holy See (Vatican City State)</option> <option value="HN">Honduras</option> <option value="HK">Hong Kong</option> <option value="HU">Hungary</option> <option value="IS">Iceland</option> <option value="IN">India</option> <option value="ID">Indonesia</option> <option value="IR">Iran, Islamic Republic of</option> <option value="IQ">Iraq</option> <option value="IE">Ireland</option> <option value="IM">Isle of Man</option> <option value="IL">Israel</option> <option value="IT">Italy</option> <option value="JM">Jamaica</option> <option value="JP">Japan</option> <option value="JE">Jersey</option> <option value="JO">Jordan</option> <option value="KZ">Kazakhstan</option> <option value="KE">Kenya</option> <option value="KI">Kiribati</option> <option value="KP">Korea, Democratic People's Republic of</option> <option value="KR">Korea, Republic of</option> <option value="KW">Kuwait</option> <option value="KG">Kyrgyzstan</option> <option value="LA">Lao People's Democratic Republic</option> <option value="LV">Latvia</option> <option value="LB">Lebanon</option> <option value="LS">Lesotho</option> <option value="LR">Liberia</option> <option value="LY">Libya</option> <option value="LI">Liechtenstein</option> <option value="LT">Lithuania</option> <option value="LU">Luxembourg</option> <option value="MO">Macao</option> <option value="MK">Macedonia, Republic of</option> <option value="MG">Madagascar</option> <option value="MW">Malawi</option> <option value="MY">Malaysia</option> <option value="MV">Maldives</option> <option value="ML">Mali</option> <option value="MT">Malta</option> <option value="MH">Marshall Islands</option> <option value="MQ">Martinique</option> <option value="MR">Mauritania</option> <option value="MU">Mauritius</option> <option value="YT">Mayotte</option> <option value="MX">Mexico</option> <option value="FM">Micronesia, Federated States of</option> <option value="MD">Moldova</option> <option value="MC">Monaco</option> <option value="MN">Mongolia</option> <option value="ME">Montenegro</option> <option value="MS">Montserrat</option> <option value="MA">Morocco</option> <option value="MZ">Mozambique</option> <option value="MM">Myanmar</option> <option value="NA">Namibia</option> <option value="NR">Nauru</option> <option value="NP">Nepal</option> <option value="NL">Netherlands</option> <option value="NC">New Caledonia</option> <option value="NZ">New Zealand</option> <option value="NI">Nicaragua</option> <option value="NE">Niger</option> <option value="NG">Nigeria</option> <option value="NU">Niue</option> <option value="NF">Norfolk Island</option> <option value="MP">Northern Mariana Islands</option> <option value="NO">Norway</option> <option value="OM">Oman</option> <option value="PK">Pakistan</option> <option value="PW">Palau</option> <option value="PS">Palestine, State of</option> <option value="PA">Panama</option> <option value="PG">Papua New Guinea</option> <option value="PY">Paraguay</option> <option value="PE">Peru</option> <option value="PH">Philippines</option> <option value="PN">Pitcairn</option> <option value="PL">Poland</option> <option value="PT">Portugal</option> <option value="PR">Puerto Rico</option> <option value="QA">Qatar</option> <option value="RE">Réunion</option> <option value="RO">Romania</option> <option value="RU">Russian Federation</option> <option value="RW">Rwanda</option> <option value="BL">Saint Barthélemy</option> <option value="SH">Saint Helena, Ascension and Tristan da Cunha</option> <option value="KN">Saint Kitts and Nevis</option> <option value="LC">Saint Lucia</option> <option value="MF">Saint Martin (French part)</option> <option value="PM">Saint Pierre and Miquelon</option> <option value="VC">Saint Vincent and the Grenadines</option> <option value="WS">Samoa</option> <option value="SM">San Marino</option> <option value="ST">Sao Tome and Principe</option> <option value="SA">Saudi Arabia</option> <option value="SN">Senegal</option> <option value="RS">Serbia</option> <option value="SC">Seychelles</option> <option value="SL">Sierra Leone</option> <option value="SG">Singapore</option> <option value="SX">Sint Maarten (Dutch part)</option> <option value="SK">Slovakia</option> <option value="SI">Slovenia</option> <option value="SB">Solomon Islands</option> <option value="SO">Somalia</option> <option value="ZA">South Africa</option> <option value="GS">South Georgia and the South Sandwich Islands</option> <option value="ES">Spain</option> <option value="LK">Sri Lanka</option> <option value="SD">Sudan</option> <option value="SR">Suriname</option> <option value="SS">South Sudan</option> <option value="SJ">Svalbard and Jan Mayen</option> <option value="SZ">Swaziland</option> <option value="SE">Sweden</option> <option value="CH">Switzerland</option> <option value="SY">Syrian Arab Republic</option> <option value="TW">Taiwan</option> <option value="TJ">Tajikistan</option> <option value="TZ">Tanzania</option> <option value="TH">Thailand</option> <option value="TL">Timor-Leste</option> <option value="TG">Togo</option> <option value="TK">Tokelau</option> <option value="TO">Tonga</option> <option value="TT">Trinidad and Tobago</option> <option value="TN">Tunisia</option> <option value="TR">Turkey</option> <option value="TM">Turkmenistan</option> <option value="TC">Turks and Caicos Islands</option> <option value="TV">Tuvalu</option> <option value="UG">Uganda</option> <option value="UA">Ukraine</option> <option value="AE">United Arab Emirates</option> <option value="GB">United Kingdom</option> <option value="US" selected="selected">United States</option> <option value="UM">United States Minor Outlying Islands</option> <option value="UY">Uruguay</option> <option value="UZ">Uzbekistan</option> <option value="VU">Vanuatu</option> <option value="VE">Venezuela</option> <option value="VN">Viet Nam</option> <option value="VG">Virgin Islands, British</option> <option value="VI">Virgin Islands, U.S.</option> <option value="WF">Wallis and Futuna</option> <option value="EH">Western Sahara</option> <option value="YE">Yemen</option> <option value="ZM">Zambia</option> <option value="ZW">Zimbabwe</option> </select><script> $('#country_selector_quote-2766').selectize(); </script><br /> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">State</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][state]" id="state-2766" maxlength="3" type="text"/><br /> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Email <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][email]" placeholder="email@address.com" maxlength="255" type="email" id="QuoteEmail" required="required"/> </div> </div> <div class="row collapse" id="email_v"> <div class="small-3 large-2 columns"> <span class="prefix">Email verification<sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][email_v]" autocomplete="nope" type="text" id="QuoteEmailV"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Comment</span> </div> <div class="small-9 large-10 columns"> <textarea name="data[Quote][comment]" placeholder="Additional comments" cols="30" rows="6" id="QuoteComment"></textarea> </div> </div> <!------------SERVICES PARTICULAR FORM START----------------> <!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES-----> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <div class="recaptcha"><div id="recaptcha6766db8972d0f"></div></div> </div> </div> <br /> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <button id="submit_btn-2766" class="alert button expand" form="Quote-2766" type="submit">Contact me</button> </div> </div> </form><script> var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c'; function postToPardot(formAction, id) { $('#pardot-form-handler').load(function(){ $('#Quote-' + id).attr('action', formAction); $('#Quote-' + id).submit(); }); $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize()); } $(document).ready(function() { 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(SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-2766 #state-2766").parent().parent().show(); } else { $("#Quote-2766 #state-2766").parent().parent().hide(); $("#Quote-2766 #state-2766").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-2766" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="bioruptor-one-sonication-device" data-reveal-id="quoteModal-2766" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Diagenode One sonication device</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/50-ul-microfluidic-chips"><img src="/img/product/shearing_technologies/br-one-20ul-chip.png" alt="Bioruptor One - 50 ul chip" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C30150002</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2775" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/2775" id="CartAdd/2775Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2775" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 50 ?l Microfluidic Chips for the One</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('50 ?l Microfluidic Chips for the One', 'C30150002', '80', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('50 ?l Microfluidic Chips for the One', 'C30150002', '80', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="50-ul-microfluidic-chips" data-reveal-id="cartModal-2775" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">50 μl Microfluidic Chips for the One</h6> </div> </div> </li> ' $related = array( 'id' => '2775', 'antibody_id' => null, 'name' => '50 ?l Microfluidic Chips for the One', 'description' => '<p><span>The 50 μl microfluidic chips were specifically designed for efficient DNA shearing (200bp - 1kb) with the One. 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The fully integrated cooling system and the all-new<sup> </sup>20 and 50 µl microfluidic chips have been enhanced to deliver the highest performance. </p> <p></p> </div> </div> <div class="row"> <div class="small-12 medium-12 large-12 columns text-left"> <ul> <li><span>Compact size</span></li> <li><span>Small volume DNA and Chromatin shearing for optimal sample prep</span></li> <li><span>Fully integrated system</span></li> </ul> </div> </div>', 'label1' => 'Highly reproducible DNA shearing ', 'info1' => '<p>The One provide the most consistent and ideal shearing (200 bp - 1 kb) to ensure subsequent high quality NGS data.</p> <p></p> <div class="row"> <div class="small-3 medium-3 large-3 columns img-center"> <p><small><strong>High and homogeneous shearing performance.</strong> Fragment Analyzer<sup>TM</sup> (Advanced Analytical Technologies, Inc.) profile showing the tight distribution for 8 independent DNA samples prepared with the One.</small></p> </div> <div class="small-9 medium-9 large-9 columns img-center"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-200bp-dna-shearing.jpg" alt="" height="422" width="942" /></div> </div>', 'label2' => 'Unbiased DNA shearing for Next-Generation Sequencing', 'info2' => '<p>The One is specifically designed to prepare high-quality sheared DNA prior to create unbiaised libraries with even distribution and representation of the genome. 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Epidermis</i> DNA shearing for best coverage.</strong> Overall distribution of read depths for a AT-rich genome.</small></p> </div> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-read-depth-bper.png" alt="" height="942" width="942" /> <p><small><strong>Unbiaised <i>B. Pertussis</i> DNA shearing for best coverage.</strong> Overall distribution of read depths for a CG-rich genome.</small></p> </div> </div>', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B01070001', 'old_catalog_number' => '', 'sf_code' => 'B01070001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '12000', 'price_USD' => '13000', 'price_GBP' => '10500', 'price_JPY' => '1879800', 'price_CNY' => 'A lot', 'price_AUD' => '32500', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-one-sonication-device', 'meta_title' => 'Diagenode One sonication device for small volume | Diagenode', 'meta_keywords' => 'Bioruptor® One, sonication,sonicator,next generation sequencing', 'meta_description' => ' Diagenode's new compact size One sonication system provides Highly reproducible DNA shearing of sample size 20 µl or 50 µl for optimal NGS library prep', 'modified' => '2023-12-20 14:20:16', 'created' => '2015-11-09 12:10:17', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2775', 'antibody_id' => null, 'name' => '50 ?l Microfluidic Chips for the One', 'description' => '<p><span>The 50 μl microfluidic chips were specifically designed for efficient DNA shearing (200bp - 1kb) with the One. 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This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div> </div> <p></p> <div class="row"> <div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div> <div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div> <div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div> <div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'dna-rna-shearing', 'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ', 'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.', 'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode', 'modified' => '2017-12-08 14:44:11', 'created' => '2014-10-29 12:45:41', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '106', 'position' => '1', 'parent_id' => '108', 'name' => 'Diagenode One accessories ', 'description' => '<p><span>All you need for the smooth running of the Diagenode</span><span> One.</span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'diagenode-one-accessories', 'cookies_tag_id' => null, 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => '', 'modified' => '2016-03-15 11:06:21', 'created' => '2015-12-04 12:37:32', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array(), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1739', 'name' => 'product/shearing_technologies/br-one-50ul-chip.png', 'alt' => 'Bioruptor One - 20 ul chip', 'modified' => '2016-03-15 11:01:52', 'created' => '2015-12-04 16:46:38', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/bioruptor-one-sonication-device"><img src="/img/product/shearing_technologies/br-one-profil.png" alt="Bioruptor One" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01070001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-2766" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Diagenode One sonication device</strong>. 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columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C30150002</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2775" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/2775" id="CartAdd/2775Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2775" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" 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Pertussis</i> DNA shearing for best coverage.</strong> Overall distribution of read depths for a CG-rich genome.</small></p> </div> </div>', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B01070001', 'old_catalog_number' => '', 'sf_code' => 'B01070001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '12000', 'price_USD' => '13000', 'price_GBP' => '10500', 'price_JPY' => '1879800', 'price_CNY' => 'A lot', 'price_AUD' => '32500', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-one-sonication-device', 'meta_title' => 'Diagenode One sonication device for small volume | Diagenode', 'meta_keywords' => 'Bioruptor® One, sonication,sonicator,next generation sequencing', 'meta_description' => ' Diagenode's new compact size One sonication system provides Highly reproducible DNA shearing of sample size 20 µl or 50 µl for optimal NGS library prep', 'modified' => '2023-12-20 14:20:16', 'created' => '2015-11-09 12:10:17', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2775', 'antibody_id' => null, 'name' => '50 ?l Microfluidic Chips for the One', 'description' => '<p><span>The 50 μl microfluidic chips were specifically designed for efficient DNA shearing (200bp - 1kb) with the One. They are simple to use and handle.</span></p>', 'label1' => 'It includes everything you need', 'info1' => '<p>The 50 μl microfluidic chips includes everything for best DNA shearing:</p> <ul> <li>12ml syringe filled with the ultrasonic gel (1x)</li> <li>50 μl microfluidic chips (10x)</li> <li>Precut cover tape (10x)</li> </ul>', 'label2' => 'Recommended settings for DNA shearing', 'info2' => '<p><span style="font-weight: 400;">Follow our guidelines </span><a href="https://www.diagenode.com/en/dna-shearing-guide">https://www.diagenode.com/en/dna-shearing-guide</a><span style="font-weight: 400;"> and find the adequate length of sonication for your expected DNA size.</span></p>', 'label3' => '', 'info3' => '', 'format' => '10 chips', 'catalog_number' => 'C30150002', 'old_catalog_number' => '', 'sf_code' => 'C30150002-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '80', 'price_USD' => '80', 'price_GBP' => '75', 'price_JPY' => '12530', 'price_CNY' => '', 'price_AUD' => '200', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '50-ul-microfluidic-chips', 'meta_title' => '50μl Bioruptor® One Microfluidic Chips | Diagenode', 'meta_keywords' => '50μl Microfluidic Chips,Bioruptor® One,DNA shearing ', 'meta_description' => 'Diagenode offers 50 μl Bioruptor® One microfluidic chips were specifically designed for efficient DNA shearing (200bp - 1kb) with the Bioruptor® One', 'modified' => '2017-08-19 10:34:22', 'created' => '2015-12-04 13:24:25', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '13', 'position' => '10', 'parent_id' => '3', 'name' => 'DNA/RNA shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div> </div> <p></p> <div class="row"> <div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div> <div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div> <div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div> <div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. 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columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C30150002</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2775" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/2775" id="CartAdd/2775Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2775" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" 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The fully integrated cooling system and the all-new<sup> </sup>20 and 50 µl microfluidic chips have been enhanced to deliver the highest performance. </p> <p></p> </div> </div> <div class="row"> <div class="small-12 medium-12 large-12 columns text-left"> <ul> <li><span>Compact size</span></li> <li><span>Small volume DNA and Chromatin shearing for optimal sample prep</span></li> <li><span>Fully integrated system</span></li> </ul> </div> </div>', 'label1' => 'Highly reproducible DNA shearing ', 'info1' => '<p>The One provide the most consistent and ideal shearing (200 bp - 1 kb) to ensure subsequent high quality NGS data.</p> <p></p> <div class="row"> <div class="small-3 medium-3 large-3 columns img-center"> <p><small><strong>High and homogeneous shearing performance.</strong> Fragment Analyzer<sup>TM</sup> (Advanced Analytical Technologies, Inc.) profile showing the tight distribution for 8 independent DNA samples prepared with the One.</small></p> </div> <div class="small-9 medium-9 large-9 columns img-center"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-200bp-dna-shearing.jpg" alt="" height="422" width="942" /></div> </div>', 'label2' => 'Unbiased DNA shearing for Next-Generation Sequencing', 'info2' => '<p>The One is specifically designed to prepare high-quality sheared DNA prior to create unbiaised libraries with even distribution and representation of the genome. The perfect unbiased shearing enables ultra-high and uniform coverage of libraries prepared from even AT-GC (<em>E. Coli</em>), AT-rich (<em>S. Epidermis</em>) and, GC-rich (<em>B. Pertussis</em>) bacterial genomes. The whole-genome sequencing on the Illumina<sup>®</sup> platform (Hiseq instrument, 50 base long single end reads) shows an excellent distribution for all three genomes.</p> <div class="row"> <div class="small-9 medium-9 large-9 columns text-center"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-coverage-ecoli.jpg" alt="" height="262" width="942" /></div> <div class="small-3 medium-3 large-3 columns text-center"> <p><small><strong><i>E. Coli</i> genome uniform and consitent coverage.</strong> Number of matched reads to the reference genome.</small></p> </div> </div> <p></p> <div class="row"> <div class="small-9 medium-9 large-9 columns text-center"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-coverage-sepi.jpg" alt="" height="262" width="942" /></div> <div class="small-3 medium-3 large-3 columns text-center"> <p><small><strong><i>S. Epidermis</i> genome uniform and consitent coverage.</strong> Number of matched reads to the reference genome.</small></p> </div> </div> <p></p> <div class="row"> <div class="small-9 medium-9 large-9 columns text-center"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-coverage-bper.jpg" alt="" height="262" width="942" /></div> <div class="small-3 medium-3 large-3 columns text-center"> <p><small><strong><i>B. Pertussis</i> genome uniform and consitent coverage.</strong> Number of matched reads to the reference genome.</small></p> </div> </div> <div class="row"> <div class="small-12 medium-12 large-12 columns text-center"></div> <p></p> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-read-depth-ecoli.jpg" alt="" height="262" width="942" /> <p><small><strong>Unbiaised <i>E. Coli</i> DNA shearing for best coverage.</strong> Overall distribution of read depths for an even AT-GC genome.</small></p> </div> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-read-depth-sepi.jpg" alt="" height="942" width="942" /> <p><small><strong>Unbiaised <i>S. Epidermis</i> DNA shearing for best coverage.</strong> Overall distribution of read depths for a AT-rich genome.</small></p> </div> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-read-depth-bper.png" alt="" height="942" width="942" /> <p><small><strong>Unbiaised <i>B. Pertussis</i> DNA shearing for best coverage.</strong> Overall distribution of read depths for a CG-rich genome.</small></p> </div> </div>', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B01070001', 'old_catalog_number' => '', 'sf_code' => 'B01070001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '12000', 'price_USD' => '13000', 'price_GBP' => '10500', 'price_JPY' => '1879800', 'price_CNY' => 'A lot', 'price_AUD' => '32500', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-one-sonication-device', 'meta_title' => 'Diagenode One sonication device for small volume | Diagenode', 'meta_keywords' => 'Bioruptor® One, sonication,sonicator,next generation sequencing', 'meta_description' => ' Diagenode's new compact size One sonication system provides Highly reproducible DNA shearing of sample size 20 µl or 50 µl for optimal NGS library prep', 'modified' => '2023-12-20 14:20:16', 'created' => '2015-11-09 12:10:17', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2775', 'antibody_id' => null, 'name' => '50 ?l Microfluidic Chips for the One', 'description' => '<p><span>The 50 μl microfluidic chips were specifically designed for efficient DNA shearing (200bp - 1kb) with the One. They are simple to use and handle.</span></p>', 'label1' => 'It includes everything you need', 'info1' => '<p>The 50 μl microfluidic chips includes everything for best DNA shearing:</p> <ul> <li>12ml syringe filled with the ultrasonic gel (1x)</li> <li>50 μl microfluidic chips (10x)</li> <li>Precut cover tape (10x)</li> </ul>', 'label2' => 'Recommended settings for DNA shearing', 'info2' => '<p><span style="font-weight: 400;">Follow our guidelines </span><a href="https://www.diagenode.com/en/dna-shearing-guide">https://www.diagenode.com/en/dna-shearing-guide</a><span style="font-weight: 400;"> and find the adequate length of sonication for your expected DNA size.</span></p>', 'label3' => '', 'info3' => '', 'format' => '10 chips', 'catalog_number' => 'C30150002', 'old_catalog_number' => '', 'sf_code' => 'C30150002-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '80', 'price_USD' => '80', 'price_GBP' => '75', 'price_JPY' => '12530', 'price_CNY' => '', 'price_AUD' => '200', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '50-ul-microfluidic-chips', 'meta_title' => '50μl Bioruptor® One Microfluidic Chips | Diagenode', 'meta_keywords' => '50μl Microfluidic Chips,Bioruptor® One,DNA shearing ', 'meta_description' => 'Diagenode offers 50 μl Bioruptor® One microfluidic chips were specifically designed for efficient DNA shearing (200bp - 1kb) with the Bioruptor® One', 'modified' => '2017-08-19 10:34:22', 'created' => '2015-12-04 13:24:25', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '13', 'position' => '10', 'parent_id' => '3', 'name' => 'DNA/RNA shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div> </div> <p></p> <div class="row"> <div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div> <div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div> <div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div> <div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'dna-rna-shearing', 'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ', 'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.', 'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode', 'modified' => '2017-12-08 14:44:11', 'created' => '2014-10-29 12:45:41', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '106', 'position' => '1', 'parent_id' => '108', 'name' => 'Diagenode One accessories ', 'description' => '<p><span>All you need for the smooth running of the Diagenode</span><span> One.</span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'diagenode-one-accessories', 'cookies_tag_id' => null, 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => '', 'modified' => '2016-03-15 11:06:21', 'created' => '2015-12-04 12:37:32', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array(), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1739', 'name' => 'product/shearing_technologies/br-one-50ul-chip.png', 'alt' => 'Bioruptor One - 20 ul chip', 'modified' => '2016-03-15 11:01:52', 'created' => '2015-12-04 16:46:38', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/bioruptor-one-sonication-device"><img src="/img/product/shearing_technologies/br-one-profil.png" alt="Bioruptor One" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01070001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-2766" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Diagenode One sonication device</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p> </div> </div> <form action="/en/quotes/quote?id=2766" id="Quote-2766" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="2766" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="d0U3c1YwZC84dStqcXJ5RU81OXVPdz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="bioruptor-one-sonication-device" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/> <div class="row collapse"> <h2>Contact Information</h2> <div class="small-3 large-2 columns"> <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div 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(SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-2766 #state-2766").parent().parent().show(); } else { $("#Quote-2766 #state-2766").parent().parent().hide(); $("#Quote-2766 #state-2766").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-2766" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="bioruptor-one-sonication-device" data-reveal-id="quoteModal-2766" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Diagenode One sonication device</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/50-ul-microfluidic-chips"><img src="/img/product/shearing_technologies/br-one-20ul-chip.png" alt="Bioruptor One - 50 ul chip" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C30150002</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2775" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/2775" id="CartAdd/2775Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2775" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 50 ?l Microfluidic Chips for the One</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('50 ?l Microfluidic Chips for the One', 'C30150002', '80', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('50 ?l Microfluidic Chips for the One', 'C30150002', '80', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="50-ul-microfluidic-chips" data-reveal-id="cartModal-2775" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">50 μl Microfluidic Chips for the One</h6> </div> </div> </li> ' $related = array( 'id' => '2775', 'antibody_id' => null, 'name' => '50 ?l Microfluidic Chips for the One', 'description' => '<p><span>The 50 μl microfluidic chips were specifically designed for efficient DNA shearing (200bp - 1kb) with the One. 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