The 50 μl microfluidic chips includes everything for best DNA shearing:
- 12ml syringe filled with the ultrasonic gel (1x)
- 50 μl microfluidic chips (10x)
- Precut cover tape (10x)
The 50 μl microfluidic chips were specifically designed for efficient DNA shearing (200bp - 1kb) with the One. They are simple to use and handle.
TESTIMONIALAs Genomic services facility, Genoma Lab team, have a large experience, processing different samples for NGS library prep and usually those protocols start with a fragmentation step and frequently the enzimatic option works fine at the first attemp, for the majority kind of samples, Some complex samples (environmental and clinical samples) are more resistant and require more treatments with different setups, which is a problem in low amount DNA and unique samples, causing at the same time a negative impact in experimental planning, times of processing samples and cost. Because of this, the introduction of mechanical fragmentation strategy, using Diagenoder One, became more relevant in the Lab routine. Bioruptor One improved our NGS workflows, providing better quality, cost and time of experiments, with a quick, easy, versatile and clean method!
Ph.D. Carolina Sánchez & Genoma Lab team. Genomic services facility Universidad Mayor. Santiago. Chile.
The 50 μl microfluidic chips includes everything for best DNA shearing:
Follow our guidelines https://www.diagenode.com/en/dna-shearing-guide and find the adequate length of sonication for your expected DNA size.
 How to properly cite this product in your workDiagenode strongly recommends using this: 50 μl Microfluidic Chips for the One (Diagenode Cat# C30150002). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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Chile.', 'featured' => true, 'slug' => 'Genomic-services-facility-santiago', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-05-14 14:49:58', 'created' => '2018-05-14 14:49:58', 'ProductsTestimonial' => array( [maximum depth reached] ) ) ), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '<blockquote><span class="label-green" style="margin-bottom:16px;margin-left:-22px">TESTIMONIAL</span><p>As Genomic services facility, Genoma Lab team, have a large experience, processing different samples for NGS library prep and usually those protocols start with a fragmentation step and frequently the enzimatic option works fine at the first attemp, for the majority kind of samples, Some complex samples (environmental and clinical samples) are more resistant and require more treatments with different setups, which is a problem in low amount DNA and unique samples, causing at the same time a negative impact in experimental planning, times of processing samples and cost. Because of this, the introduction of mechanical fragmentation strategy, using Diagenoder One, became more relevant in the Lab routine. Bioruptor One improved our NGS workflows, providing better quality, cost and time of experiments, with a quick, easy, versatile and clean method!</p><cite>Ph.D. Carolina Sánchez & Genoma Lab team. Genomic services facility Universidad Mayor. Santiago. Chile.</cite></blockquote> ' $testimonial = array( 'id' => '71', 'name' => 'Diagenode One Carolina Sanchez', 'description' => '<p>As Genomic services facility, Genoma Lab team, have a large experience, processing different samples for NGS library prep and usually those protocols start with a fragmentation step and frequently the enzimatic option works fine at the first attemp, for the majority kind of samples, Some complex samples (environmental and clinical samples) are more resistant and require more treatments with different setups, which is a problem in low amount DNA and unique samples, causing at the same time a negative impact in experimental planning, times of processing samples and cost. Because of this, the introduction of mechanical fragmentation strategy, using Diagenoder One, became more relevant in the Lab routine. Bioruptor One improved our NGS workflows, providing better quality, cost and time of experiments, with a quick, easy, versatile and clean method!</p>', 'author' => 'Ph.D. Carolina Sánchez & Genoma Lab team. Genomic services facility Universidad Mayor. Santiago. Chile.', 'featured' => true, 'slug' => 'Genomic-services-facility-santiago', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-05-14 14:49:58', 'created' => '2018-05-14 14:49:58', 'ProductsTestimonial' => array( 'id' => '173', 'product_id' => '2775', 'testimonial_id' => '71' ) ) $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/bioruptor-one-sonication-device"><img src="/img/product/shearing_technologies/br-one-profil.png" alt="Bioruptor One" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01070001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-2766" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Diagenode One sonication device</strong>. 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columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C30150001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2774" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/2774" id="CartAdd/2774Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2774" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 20 ?l Microfluidic Chips for the One</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('20 ?l Microfluidic Chips for the One', 'C30150001', '80', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('20 ?l Microfluidic Chips for the One', 'C30150001', '80', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="20-ul-microfluidic-chips" data-reveal-id="cartModal-2774" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 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This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div> </div> <p></p> <div class="row"> <div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div> <div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div> <div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div> <div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'dna-rna-shearing', 'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ', 'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.', 'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode', 'modified' => '2017-12-08 14:44:11', 'created' => '2014-10-29 12:45:41', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '106', 'position' => '1', 'parent_id' => '108', 'name' => 'Diagenode One accessories ', 'description' => '<p><span>All you need for the smooth running of the Diagenode</span><span> One.</span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'diagenode-one-accessories', 'cookies_tag_id' => null, 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => '', 'modified' => '2016-03-15 11:06:21', 'created' => '2015-12-04 12:37:32', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array(), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1738', 'name' => 'product/shearing_technologies/br-one-20ul-chip.png', 'alt' => 'Bioruptor One - 50 ul chip', 'modified' => '2016-03-15 11:01:28', 'created' => '2015-12-04 16:46:07', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array( (int) 0 => array( 'id' => '71', 'name' => 'Diagenode One Carolina Sanchez', 'description' => '<p>As Genomic services facility, Genoma Lab team, have a large experience, processing different samples for NGS library prep and usually those protocols start with a fragmentation step and frequently the enzimatic option works fine at the first attemp, for the majority kind of samples, Some complex samples (environmental and clinical samples) are more resistant and require more treatments with different setups, which is a problem in low amount DNA and unique samples, causing at the same time a negative impact in experimental planning, times of processing samples and cost. Because of this, the introduction of mechanical fragmentation strategy, using Diagenoder One, became more relevant in the Lab routine. Bioruptor One improved our NGS workflows, providing better quality, cost and time of experiments, with a quick, easy, versatile and clean method!</p>', 'author' => 'Ph.D. Carolina Sánchez & Genoma Lab team. Genomic services facility Universidad Mayor. Santiago. Chile.', 'featured' => true, 'slug' => 'Genomic-services-facility-santiago', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-05-14 14:49:58', 'created' => '2018-05-14 14:49:58', 'ProductsTestimonial' => array( [maximum depth reached] ) ) ), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '<blockquote><span class="label-green" style="margin-bottom:16px;margin-left:-22px">TESTIMONIAL</span><p>As Genomic services facility, Genoma Lab team, have a large experience, processing different samples for NGS library prep and usually those protocols start with a fragmentation step and frequently the enzimatic option works fine at the first attemp, for the majority kind of samples, Some complex samples (environmental and clinical samples) are more resistant and require more treatments with different setups, which is a problem in low amount DNA and unique samples, causing at the same time a negative impact in experimental planning, times of processing samples and cost. Because of this, the introduction of mechanical fragmentation strategy, using Diagenoder One, became more relevant in the Lab routine. Bioruptor One improved our NGS workflows, providing better quality, cost and time of experiments, with a quick, easy, versatile and clean method!</p><cite>Ph.D. Carolina Sánchez & Genoma Lab team. Genomic services facility Universidad Mayor. Santiago. 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Bioruptor One improved our NGS workflows, providing better quality, cost and time of experiments, with a quick, easy, versatile and clean method!</p>', 'author' => 'Ph.D. Carolina Sánchez & Genoma Lab team. Genomic services facility Universidad Mayor. Santiago. Chile.', 'featured' => true, 'slug' => 'Genomic-services-facility-santiago', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-05-14 14:49:58', 'created' => '2018-05-14 14:49:58', 'ProductsTestimonial' => array( 'id' => '173', 'product_id' => '2775', 'testimonial_id' => '71' ) ) $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/bioruptor-one-sonication-device"><img src="/img/product/shearing_technologies/br-one-profil.png" alt="Bioruptor One" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01070001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-2766" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Diagenode One sonication device</strong>. 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columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C30150001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2774" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/2774" id="CartAdd/2774Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2774" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" 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The fully integrated cooling system and the all-new<sup> </sup>20 and 50 µl microfluidic chips have been enhanced to deliver the highest performance. </p> <p></p> </div> </div> <div class="row"> <div class="small-12 medium-12 large-12 columns text-left"> <ul> <li><span>Compact size</span></li> <li><span>Small volume DNA and Chromatin shearing for optimal sample prep</span></li> <li><span>Fully integrated system</span></li> </ul> </div> </div>', 'label1' => 'Highly reproducible DNA shearing ', 'info1' => '<p>The One provide the most consistent and ideal shearing (200 bp - 1 kb) to ensure subsequent high quality NGS data.</p> <p></p> <div class="row"> <div class="small-3 medium-3 large-3 columns img-center"> <p><small><strong>High and homogeneous shearing performance.</strong> Fragment Analyzer<sup>TM</sup> (Advanced Analytical Technologies, Inc.) profile showing the tight distribution for 8 independent DNA samples prepared with the One.</small></p> </div> <div class="small-9 medium-9 large-9 columns img-center"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-200bp-dna-shearing.jpg" alt="" height="422" width="942" /></div> </div>', 'label2' => 'Unbiased DNA shearing for Next-Generation Sequencing', 'info2' => '<p>The One is specifically designed to prepare high-quality sheared DNA prior to create unbiaised libraries with even distribution and representation of the genome. 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Coli</i> genome uniform and consitent coverage.</strong> Number of matched reads to the reference genome.</small></p> </div> </div> <p></p> <div class="row"> <div class="small-9 medium-9 large-9 columns text-center"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-coverage-sepi.jpg" alt="" height="262" width="942" /></div> <div class="small-3 medium-3 large-3 columns text-center"> <p><small><strong><i>S. Epidermis</i> genome uniform and consitent coverage.</strong> Number of matched reads to the reference genome.</small></p> </div> </div> <p></p> <div class="row"> <div class="small-9 medium-9 large-9 columns text-center"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-coverage-bper.jpg" alt="" height="262" width="942" /></div> <div class="small-3 medium-3 large-3 columns text-center"> <p><small><strong><i>B. Pertussis</i> genome uniform and consitent coverage.</strong> Number of matched reads to the reference genome.</small></p> </div> </div> <div class="row"> <div class="small-12 medium-12 large-12 columns text-center"></div> <p></p> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-read-depth-ecoli.jpg" alt="" height="262" width="942" /> <p><small><strong>Unbiaised <i>E. Coli</i> DNA shearing for best coverage.</strong> Overall distribution of read depths for an even AT-GC genome.</small></p> </div> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-read-depth-sepi.jpg" alt="" height="942" width="942" /> <p><small><strong>Unbiaised <i>S. Epidermis</i> DNA shearing for best coverage.</strong> Overall distribution of read depths for a AT-rich genome.</small></p> </div> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-read-depth-bper.png" alt="" height="942" width="942" /> <p><small><strong>Unbiaised <i>B. Pertussis</i> DNA shearing for best coverage.</strong> Overall distribution of read depths for a CG-rich genome.</small></p> </div> </div>', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B01070001', 'old_catalog_number' => '', 'sf_code' => 'B01070001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '12000', 'price_USD' => '13000', 'price_GBP' => '10500', 'price_JPY' => '1879800', 'price_CNY' => 'A lot', 'price_AUD' => '32500', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-one-sonication-device', 'meta_title' => 'Diagenode One sonication device for small volume | Diagenode', 'meta_keywords' => 'Bioruptor® One, sonication,sonicator,next generation sequencing', 'meta_description' => ' Diagenode's new compact size One sonication system provides Highly reproducible DNA shearing of sample size 20 µl or 50 µl for optimal NGS library prep', 'modified' => '2023-12-20 14:20:16', 'created' => '2015-11-09 12:10:17', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2774', 'antibody_id' => null, 'name' => '20 ?l Microfluidic Chips for the One', 'description' => '<p>The 20 μl microfluidic chips were specifically designed for efficient DNA shearing (200bp - 1kb) with the One. They are simple to use and handle.</p>', 'label1' => 'It includes everything you need', 'info1' => '<p>The 20 μl microfluidic chips includes everything for best DNA shearing:</p> <ul style="list-style-type: circle;"> <li>12ml syringe filled with the ultrasonic gel (1x)</li> <li>20 μl microfluidic chips (10x)</li> <li>Precut cover tape (10x)</li> </ul>', 'label2' => 'Recommended settings for DNA shearing', 'info2' => '<p><span>Follow our guidelines </span><a href="https://www.diagenode.com/en/dna-shearing-guide"><span>https://www.diagenode.com/en/dna-shearing-guide</span></a><span> and find the adequate length of sonication for your expected DNA size.</span></p>', 'label3' => '', 'info3' => '', 'format' => '10 chips', 'catalog_number' => 'C30150001', 'old_catalog_number' => '', 'sf_code' => 'C30150001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '80', 'price_USD' => '80', 'price_GBP' => '75', 'price_JPY' => '12530', 'price_CNY' => '', 'price_AUD' => '200', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '20-ul-microfluidic-chips', 'meta_title' => '20 μl Bioruptor® One Microfluidic Chips | Diagenode', 'meta_keywords' => 'Microfluidic Chips,Bioruptor® One,DNA shearing,DIagenode', 'meta_description' => 'Diagenode offers 20 μl Bioruptor® One microfluidic chips were specifically designed for efficient DNA shearing (200bp - 1kb) with the Bioruptor® One.', 'modified' => '2017-08-19 10:33:57', 'created' => '2015-12-04 13:14:44', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '13', 'position' => '10', 'parent_id' => '3', 'name' => 'DNA/RNA shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div> </div> <p></p> <div class="row"> <div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div> <div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div> <div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div> <div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'dna-rna-shearing', 'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ', 'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.', 'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode', 'modified' => '2017-12-08 14:44:11', 'created' => '2014-10-29 12:45:41', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '106', 'position' => '1', 'parent_id' => '108', 'name' => 'Diagenode One accessories ', 'description' => '<p><span>All you need for the smooth running of the Diagenode</span><span> One.</span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'diagenode-one-accessories', 'cookies_tag_id' => null, 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => '', 'modified' => '2016-03-15 11:06:21', 'created' => '2015-12-04 12:37:32', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array(), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1738', 'name' => 'product/shearing_technologies/br-one-20ul-chip.png', 'alt' => 'Bioruptor One - 50 ul chip', 'modified' => '2016-03-15 11:01:28', 'created' => '2015-12-04 16:46:07', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array( (int) 0 => array( 'id' => '71', 'name' => 'Diagenode One Carolina Sanchez', 'description' => '<p>As Genomic services facility, Genoma Lab team, have a large experience, processing different samples for NGS library prep and usually those protocols start with a fragmentation step and frequently the enzimatic option works fine at the first attemp, for the majority kind of samples, Some complex samples (environmental and clinical samples) are more resistant and require more treatments with different setups, which is a problem in low amount DNA and unique samples, causing at the same time a negative impact in experimental planning, times of processing samples and cost. Because of this, the introduction of mechanical fragmentation strategy, using Diagenoder One, became more relevant in the Lab routine. Bioruptor One improved our NGS workflows, providing better quality, cost and time of experiments, with a quick, easy, versatile and clean method!</p>', 'author' => 'Ph.D. Carolina Sánchez & Genoma Lab team. Genomic services facility Universidad Mayor. Santiago. Chile.', 'featured' => true, 'slug' => 'Genomic-services-facility-santiago', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-05-14 14:49:58', 'created' => '2018-05-14 14:49:58', 'ProductsTestimonial' => array( [maximum depth reached] ) ) ), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '<blockquote><span class="label-green" style="margin-bottom:16px;margin-left:-22px">TESTIMONIAL</span><p>As Genomic services facility, Genoma Lab team, have a large experience, processing different samples for NGS library prep and usually those protocols start with a fragmentation step and frequently the enzimatic option works fine at the first attemp, for the majority kind of samples, Some complex samples (environmental and clinical samples) are more resistant and require more treatments with different setups, which is a problem in low amount DNA and unique samples, causing at the same time a negative impact in experimental planning, times of processing samples and cost. Because of this, the introduction of mechanical fragmentation strategy, using Diagenoder One, became more relevant in the Lab routine. Bioruptor One improved our NGS workflows, providing better quality, cost and time of experiments, with a quick, easy, versatile and clean method!</p><cite>Ph.D. Carolina Sánchez & Genoma Lab team. Genomic services facility Universidad Mayor. Santiago. Chile.</cite></blockquote> ' $testimonial = array( 'id' => '71', 'name' => 'Diagenode One Carolina Sanchez', 'description' => '<p>As Genomic services facility, Genoma Lab team, have a large experience, processing different samples for NGS library prep and usually those protocols start with a fragmentation step and frequently the enzimatic option works fine at the first attemp, for the majority kind of samples, Some complex samples (environmental and clinical samples) are more resistant and require more treatments with different setups, which is a problem in low amount DNA and unique samples, causing at the same time a negative impact in experimental planning, times of processing samples and cost. Because of this, the introduction of mechanical fragmentation strategy, using Diagenoder One, became more relevant in the Lab routine. Bioruptor One improved our NGS workflows, providing better quality, cost and time of experiments, with a quick, easy, versatile and clean method!</p>', 'author' => 'Ph.D. Carolina Sánchez & Genoma Lab team. Genomic services facility Universidad Mayor. Santiago. Chile.', 'featured' => true, 'slug' => 'Genomic-services-facility-santiago', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-05-14 14:49:58', 'created' => '2018-05-14 14:49:58', 'ProductsTestimonial' => array( 'id' => '173', 'product_id' => '2775', 'testimonial_id' => '71' ) ) $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/bioruptor-one-sonication-device"><img src="/img/product/shearing_technologies/br-one-profil.png" alt="Bioruptor One" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01070001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-2766" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Diagenode One sonication device</strong>. 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columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C30150001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2774" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/2774" id="CartAdd/2774Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2774" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 20 ?l Microfluidic Chips for the One</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('20 ?l Microfluidic Chips for the One', 'C30150001', '80', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('20 ?l Microfluidic Chips for the One', 'C30150001', '80', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="20-ul-microfluidic-chips" data-reveal-id="cartModal-2774" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">20 μl Microfluidic Chips for the One</h6> </div> </div> </li> ' $related = array( 'id' => '2774', 'antibody_id' => null, 'name' => '20 ?l Microfluidic Chips for the One', 'description' => '<p>The 20 μl microfluidic chips were specifically designed for efficient DNA shearing (200bp - 1kb) with the One. 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(Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div> <div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. 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Chile.', 'featured' => true, 'slug' => 'Genomic-services-facility-santiago', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-05-14 14:49:58', 'created' => '2018-05-14 14:49:58', 'ProductsTestimonial' => array( [maximum depth reached] ) ) ), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '<blockquote><span class="label-green" style="margin-bottom:16px;margin-left:-22px">TESTIMONIAL</span><p>As Genomic services facility, Genoma Lab team, have a large experience, processing different samples for NGS library prep and usually those protocols start with a fragmentation step and frequently the enzimatic option works fine at the first attemp, for the majority kind of samples, Some complex samples (environmental and clinical samples) are more resistant and require more treatments with different setups, which is a problem in low amount DNA and unique samples, causing at the same time a negative impact in experimental planning, times of processing samples and cost. Because of this, the introduction of mechanical fragmentation strategy, using Diagenoder One, became more relevant in the Lab routine. Bioruptor One improved our NGS workflows, providing better quality, cost and time of experiments, with a quick, easy, versatile and clean method!</p><cite>Ph.D. Carolina Sánchez & Genoma Lab team. Genomic services facility Universidad Mayor. Santiago. 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Bioruptor One improved our NGS workflows, providing better quality, cost and time of experiments, with a quick, easy, versatile and clean method!</p>', 'author' => 'Ph.D. Carolina Sánchez & Genoma Lab team. Genomic services facility Universidad Mayor. Santiago. Chile.', 'featured' => true, 'slug' => 'Genomic-services-facility-santiago', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-05-14 14:49:58', 'created' => '2018-05-14 14:49:58', 'ProductsTestimonial' => array( 'id' => '173', 'product_id' => '2775', 'testimonial_id' => '71' ) ) $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/bioruptor-one-sonication-device"><img src="/img/product/shearing_technologies/br-one-profil.png" alt="Bioruptor One" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01070001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-2766" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Diagenode One sonication device</strong>. 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(SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-2766 #state-2766").parent().parent().show(); } else { $("#Quote-2766 #state-2766").parent().parent().hide(); $("#Quote-2766 #state-2766").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-2766" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="bioruptor-one-sonication-device" data-reveal-id="quoteModal-2766" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Diagenode One sonication device</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/20-ul-microfluidic-chips"><img src="/img/product/shearing_technologies/br-one-50ul-chip.png" alt="Bioruptor One - 20 ul chip" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C30150001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2774" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/2774" id="CartAdd/2774Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2774" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 20 ?l Microfluidic Chips for the One</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('20 ?l Microfluidic Chips for the One', 'C30150001', '80', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('20 ?l Microfluidic Chips for the One', 'C30150001', '80', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="20-ul-microfluidic-chips" data-reveal-id="cartModal-2774" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">20 μl Microfluidic Chips for the One</h6> </div> </div> </li> ' $related = array( 'id' => '2774', 'antibody_id' => null, 'name' => '20 ?l Microfluidic Chips for the One', 'description' => '<p>The 20 μl microfluidic chips were specifically designed for efficient DNA shearing (200bp - 1kb) with the One. 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