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<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
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<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
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<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
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<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
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<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
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<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
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<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
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<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
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<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
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<p>The Diagenode <strong>µChIPmentation Kit for Histones</strong> is optimized to perform ChIP-seq on as little as <strong>10.000 cells</strong> from cell fixation to purified libraries. To <strong>reduce DNA lost</strong> the number of sample transfer from tube to tube has been limited: during whole workflow - from cell fixation to library prurification - only 3 tubes per sample are used. Reduced number of steps, reduced number of sample transfer and <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation" target="_blank">ChIPmentation technology</a> itself enable for <strong>efficient and robust ChIP-seq</strong> on <strong>limited amount of sample</strong>. The kit µChIPmentation for Histones includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The primer indexes for multiplexing are not included in the kit and have to be purchase separately - <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">read more</a>.</p>
<div class="extra-spaced">
<h3>Benefits of the µChIPmentation system for histone ChIP-seq</h3>
<ul>
<li><strong>Easier</strong> and <strong>faster</strong> than classical ChIP-seq</li>
<li>Optimized for <strong>low input samples</strong>: as little as 10,000 cells</li>
<li>Protocol optimized for <strong>FACS-sorted cells</strong></li>
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<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"><i class="fa fa-caret-right"></i> Workflow of µChIPmentation for Histones - Read more</a>
<div id="workflow" class="content">
<div class="extra-spaced"><center><img src="https://www.diagenode.com/img/product/kits/workflow-micro-chipmentation.png" /></center></div>
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<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns"><strong>A.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1A.png" /></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-5 large-5 columns" columns=""><strong>B.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1B.png" /></div>
<div class="small-12 medium-7 large-7 columns">
<p style="text-align: left;"><strong>Figure 1. Comparison between ChIPmentation and µChIPmentation </strong><br />ChIPmentation: chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011000) and diluted chromatin from 500.000 cells was used for the immunoprecipitation. µChIPmentation: chromatin preparation and immunoprecipitation have been performed on 10.000 K562 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032). The Diagenode antibody targeting H3K27me3 (Cat. No. C15410195) was used.<br /> A. Distribution of the ChIPmentation and µChIPmentation readsets in a representative region of the genome (in duplicates). B. Comparison of the top 40% peaks from µChIPmentation (10.000 cells) with dataset generated with ChIPmentation (500.000 cells).</p>
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<p><strong>Figure 2. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
</div>
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<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig3.png" /></div>
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<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 3. Integrative genomics viewer (IGV) visualization of from ChIP-seq experiments using H3K27me3 antibody (Diagenode, Cat. No. <span>C15410195</span>) and 10.000 cells of K562 cells per immunoprecipitation.<br /></strong>Cells were FACS-sorted and ChIP has been performed accordingly to µChIPmentation protocol. Batch chromatin preparation followed by immunoprecipitation has been used per comparison as indicated.</p>
</div>
</div>
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'label2' => 'Additional solutions for µChIPmentation Kit for Histones',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit - High SDS</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for multiplexing:</p>
<ul class="no-bullet">
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries" target="_blank">24 SI for Tagmented libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries" target="_blank">8 SI for Tagmented libraries Cat. No. C01011033</a></li>
<li><em></em><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for Tagmented libraries, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
<li></li>
</ul>',
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'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">
<div align="center"><video width="400" height="250" autoplay="autoplay" muted="" loop="loop" controls="controls" src="https://www.diagenode.com/videos/chipmentation-dgo.mp4" frameborder="0" allowfullscreen="allowfullscreen"></video></div>
<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
</div>
</div>
<p>Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fragments in one step. In our ChIPmentation technology we combine chromatin immunoprecipitation and tagmentation in one streamlined workflow where the tagmentation step occurs directly on chromatin. The <strong>TAG Kit for ChIPmentation</strong> has been developed for researchers who would like to perform ChIPmentation using their own validated ChIP protocol. Our TAG Kit for ChIPmentation includes reagents for <strong>tagmentation-based library preparation</strong> and is compatible with any ChIP protocol based on magnetic beads. The <strong>primer</strong> <strong>indexes for multiplexing</strong> must be <strong>purchased separately</strong> and are available as a reference:<br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank"><br /></a></p>',
'label1' => 'Characteristics',
'info1' => '<h3>Comparing to classical ChIP-seq, ChIPmentation enables:</h3>
<ul>
<li>An easier protocol</li>
<li>Faster time to results</li>
<li>Flexibility – the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows you to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
</ul>
<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
<p><small>*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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'name' => 'ATAC-seq kit',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/atacseq-kit-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p><strong>ATAC-seq</strong>, Assay for<span> </span><strong>T</strong>ransposase-<strong>A</strong>ccessible<span> </span><strong>C</strong>hromatin, followed by next generation sequencing, is a key technology for genome-wide mapping of accessible chromatin. The technology is based on the use of the<span> </span><strong>transposase Tn5</strong><span> </span>which cuts exposed open chromatin and simultaneously ligates adapters for subsequent amplification and sequencing. ATAC-seq methods allow you to:</p>
<ul>
<li> Gain insight into gene regulation and understand open chromatin signatures</li>
<li> Determine nucleosome positions at single nucleotide resolution</li>
<li> Uncover transcription factor (TF) occupancy</li>
</ul>
</div>
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<p>Diagenode’s<span> </span><b>ATAC-</b><b>seq</b><b><span> </span>kit<span> </span></b>is based on a highly validated protocol optimized for<span> </span><b>50,000<span> </span></b><b>cells</b><b><span> </span>per<span> </span></b><b>reaction</b>. The kit includes the reagents for cell lysis and nuclei extraction, tagmentation and DNA purification as well as for library amplification. The <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries">primer indexes for multiplexing</a> are not included in the kit and must be purchased separately.</p>
<h4><span style="font-weight: 400;">ATAC-seq kit features:</span></h4>
<ul>
<li><b>Cell<span> </span></b><b>requirement</b><b>:<span> </span></b><b>50,000<span> </span></b><b>cells /<span> </span></b><b>rxn</b></li>
<li><b>Robust protocol<span> </span></b>with<span> </span><b>high reproducibility<span> </span></b>between replicates and repetitive experiments</li>
<li><strong>Easy</strong><span> </span>and<span> </span><b>efficient DNA capture<span> </span></b>after the tagmentation reaction using Diagenode`s MicroChIP DiaPure columns (included)</li>
<li>Additional qPCR step to determine the number of cycles needed for library amplification: </li>
<ul type="”square”">
<li><b>Avoids<span> </span></b><b>over-amplification</b></li>
<li>Allows adaptation/flexibility for<span> </span><b>more challenging samples<span> </span></b>to succeed with library prep.</li>
<li>Gives<span> </span><strong>early indication</strong><span> </span>if the experiment does not work (no qPCR amplification)</li>
</ul>
</ul>
<p>Looking for ATAC-seq on tissue? Please, go to: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080006">ATAC-seq package for tissue</a></p>',
'label1' => 'Method overview',
'info1' => '<p><strong>ATAC-seq</strong>, <strong>A</strong>ssay for <strong>T</strong>ransposase-<strong>A</strong>ccessible <strong>C</strong>hromatin, followed by next generation sequencing, is a key technology to easily identify the <strong>open regions of the chromatin.</strong> The protocol consists of <strong>3 steps</strong>: <strong>nuclei preparation</strong>, <strong>tagmentation</strong> and <strong>library amplification</strong>. First, the cells undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq.png" alt="ATAC-seq kit workflow" width="600px" caption="false" /></p>',
'label2' => 'Example of results',
'info2' => '<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig1.png" alt="library prepared with the Diagenode ATAC-seq kit " width="500px" caption="false" /></p>
<p><strong>Figure 1.</strong>Representative Bioanalyzer profile of an ATAC-seq library prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells.</p>
<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig2.png" alt="Diagenode ATAC-seq kit " caption="false" width="951" height="148" /></p>
<p><strong>Figure 2.</strong> Main ATAC-seq alignment and peak calling statistics of 3 replicates prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells. (Mapping efficiency: Percentage of non-mitochondrial reads that mapped to the reference genome. Uniquely mapped ratio: Proportion of mapped reads that map to only one location on the reference genome (hg19). Peaks: Number of peaks (open chromatin regions) identified by MACS2 for each sample. FRiP - Fraction of reads in peaks: Percentage of reads in peaks, with respect to the number of uniquely mapped reads. Sequencing was realized in paired-end mode 50 base pairs (PE50) on an Illumina NovaSeq6000.)</p>
<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig3a.png" alt="Assay for Transposase-Accessible Chromatin" width="500px" caption="false" /></p>
<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig3b.png" alt="Assay for Transposase-Accessible Chromatin" width="500px" caption="false" /></p>
<p><strong>Figure 3</strong> Sequencing profiles of ATAC-seq library (3 replicates) prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells.</p>
<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig4.png" alt=" open chromatin regions" caption="false" width="383" height="739" /></p>
<p><strong>Figure 4. </strong><br /> Heatmap around TSS of three ATAC-seq replicates prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells.</p>',
'label3' => 'Additional solutions for ATAC-seq kit',
'info3' => '<p><a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries">Primer indexes for tagmented libraries</a></p>
<p>Magnetic rack:<span> </span><a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag</a><a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit"><span> </span>0.2 ml – Cat. No. B04000001</a></p>
<p>Additional supplies (included in the kit and available separately):</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase</a><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30"><span> </span>(Tn5 transposase)<span> </span></a><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">loaded</a><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">, Cat. No. C01070012</a></li>
<li><a href="https://www.diagenode.com/en/p/tagmentation-buffer-2x">Tagmentation</a><a href="https://www.diagenode.com/en/p/tagmentation-buffer-2x"><span> </span>Buffer (2x), Cat. No. C01019043</a></li>
<li><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP</a><span> </span><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">DiaPure</a><span> </span><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">columns</a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">, Cat. No. C03040001</a></li>
</ul>',
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'meta_title' => 'ATAC-seq kit for open chromatin assessment C01080001 | Diagenode ',
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'meta_description' => 'Diagenode’s ATAC-seq kit provides a robust protocol for assessing genome-wide chromatin accessibility',
'modified' => '2024-10-21 10:11:12',
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'id' => '3220',
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'name' => 'iDeal CUT&Tag kit for Histones <br /> Compatible with histones and some non-histone proteins',
'description' => '<p><a href="https://www.diagenode.com/files/application_notes/AN-iDealCUTandTag.pdf"><img src="https://www.diagenode.com/img/banners/cutandtag-appnote.png" /></a></p>
<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p><strong>CUT&Tag-sequencing</strong> (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins.</p>
<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
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<p>The Diagenode’s <strong>iDeal CUT&Tag kit for Histones</strong> provides an optimized protocol for a rapid chromatin profiling on <strong>histone marks</strong> and some <strong>non-histone proteins</strong>. The protocol is optimized for native cells (<strong>10,000-300,000</strong> cells per reaction) and can be completed within 1.5 days. The kit includes all reagents for cell processing, including CoA beads, pA-Tn5 and the DNA purification module. The antibodies (secondary antibodies, control antibodies) as well as primer indexes for multiplexing must be purchased separately.</p>
<p><strong>For a complete CUT&Tag protocol the following items must be purchased:</strong></p>
<ul>
<li><strong>iDeal CUT&Tag kit for Histones</strong> – including all reagents for CUT&Tag workflow (buffers, pA-Tn5, CoA beads, DNA purification)</li>
<li><strong>Antibody package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a></strong> or <strong><a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></strong> - including the secondary antibody, positive and negative control antibodies and primers</li>
<li><strong><a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries">Primer indexes for tagmented libraries</a></strong> – for multiplexing up to 72 samples</li>
</ul>
<h3>iDeal CUT&Tag Kit features:</h3>
<ul>
<li><strong>Rapid</strong> and <strong>easy </strong>chromatin profiling assay for <strong>histones </strong>and<strong> some non-histone proteins</strong></li>
<ul style="margin-bottom: 0;">
<li>No chromatin preparation</li>
<li>Easy sample handling due to ConA magnetic beads</li>
<li>Integrated library prep</li>
</ul>
<li><strong>Low cell number</strong>: 10,000-300,000 cells</li>
<li><strong>Accurate amplification</strong> due to intermediate quantification step</li>
<li><strong>High resolution</strong> and <strong>sensitivity</strong></li>
<li><strong>Lower sequencing depth</strong></li>
</ul>
<p>The quality of antibody used in CUT&Tag is one of the crucial factors for assay success. The antibodies with confirmed high specificity will target only the protein of interest, enabling real results. Check out our selection of <strong><a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">antibodies validated in CUT&Tag</a>.</strong></p>
<p>Looking for a standalone pA-Tn5? <a href="https://www.diagenode.com/en/products/view/3064">Read more</a>.</p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Method overview',
'info1' => '<p>The iDeal CUT&Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode’s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode’s Primer Indexes for tagmented libraries.</p>
<p></p>
<p><img alt=" pA-Tn5 Antibody package for CUT & Tag" src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></p>',
'label2' => 'Examples of results - histone marks',
'info2' => '<p>Successful CUT&Tag results showing a low background with high region-specific enrichment are presented below. Chromatin profiling has been performed on 50,000 K562 cells, using the Diagenode’s iDeal CUT&Tag kit for Histones (Cat. No. C01070020), the Antibody Package for CUT&Tag anti rabbit (Cat. No. C01070022), the 24 UDI for Tagmented Libraries (Cat.No. C01011034) and H3K4me3 (Cat. No. C15410003), H3K27me3 (Cat. No. C15410069) or H3H9me3 antibodies (Cat. No., C15410193) as indicated. The libraries were sequenced on Illumina’s NovaSeq6000 in 2x50 bp mode.</p>
<p><br /> <img alt="CUT&Tag-sequencing" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig1a.png" /> <img alt="CUT&Tag kit for Histones" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig1b.png" /> <img alt="Cleavage Under Targets and Tagmentation" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig1c.png" /></p>
<p><strong>Figure 1.</strong> Typical library profiles (Agilent Fragment traces) generated by the iDeal CUT&Tag protocol using 50,000 K562 cells and H3K27me3 (top), H3K4me4 (middle) primary antibodies and IgG control (bottom). Sharp peak at around 40 bp is an excess of <strong>free oligonucleotide used for pA-Tn5 loading</strong>.</p>
<p></p>
<p><br /><br /></p>
<div class="row">
<div class="small-4 columns">
<p><img alt="iDeal CUT&Tag kit for Histones" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig2.png" /></p>
</div>
<div class="small-8 columns">
<p><strong>Figure 2.</strong> Enrichments at TSS of the CUT&Tag libraries. The heatmap shows the enrichment around 3kb upstream and downstream of the TSS for H3K4me3. H3K4me3 as an active chromatin mark is associated with active promoters shows a narrow enrichment pattern.</p>
</div>
</div>
<p><br /><br /></p>
<p><br /> <img alt="iDeal CUT&Tag experiments of K562 cells " src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig3.png" /></p>
<p><strong>Figure 3.</strong> Sequencing profiles of the CUT&Tag libraries. Integrative genomics viewer (IGV) visualization of CUT&Tag experiments using 50,000 of K562 cells and H3K4me3 (blue), H3K27me3 (red) or H3K9me3 (green).</p>
<p><br /><br /></p>
<p><br /> <img alt="CUT&Tag experiments using 10,000 K562 cells" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig4.png" /></p>
<p><strong>Figure 4.</strong> Sequencing profiles of the CUT&Tag libraries. Integrative genomics viewer (IGV) visualization of CUT&Tag experiments using 50,000 (blue) or 10,000 (red)) of K562 cells and H3K27me3 antibody.</p>',
'label3' => 'Examples of results - transcription factors',
'info3' => '<p>Diagenode's<strong><span> iDeal CUT&Tag Kit for Histones</span></strong><span>, </span>developed for an efficient chromatin profiling on histone marks, can be used for profiling of <span>some transcription factors and co-factors using a mild fixation as described in the manual. </span></p>
<p><strong><span>Successful CUT&Tag results using CTCF and Suz12 antibodies and iDeal CUT&Tag Kit for Histones. </span></strong></p>
<p></p>
<p><span>Chromatin profiling of <strong>Suz12</strong> has been performed on 50,000 of fixed Mouse ES-E14TG2a cells (kindly provided by Luciano Di Croce, CRG, Spain) accordingly to the protocol of Diagenode’s iDeal CUT&Tag kit for Histones (Cat. No. C01070020). Antibody Package for CUT&Tag anti rabbit (Cat. No. C01070022) and the 24 UDI for Tagmented Libraries (Cat. No. C01011034) were used. The libraries were sized using Fragment Analyzer (Agilent) (triplicates, Figure 1, top). Relative enrichment has been confirmed by qPCR using know positive (T_Bra) and negative (Actb) loci (Figure 1, bottom). Libraries were sequenced on Illumina’s NovaSeq6000 in 2x50 bp mode.</span></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig1a.jpg" /><br /> <img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig1b.jpg" /></p>
<p><strong>Figure 1.</strong> Typical library profiles (top) and relative enrichment (bottom) generated by the iDeal CUT&Tag protocol using 50,000 ES-E14TG2a cells and Suz12 Antibody and IgG control (bottom). Sharp peak at around 40 bp is an excess of free oligonucleotide used for pA-Tn5 loading which does not interfere with the sequencing.</p>
<p></p>
<p>Pictures of representative loci were taken on Integrative Genome Viewer (IGV) for CTCF demonstrating the presence of high signal at promoter regions.</p>
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-Fig2a.jpg" /><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-Fig2b.jpg" /> <img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-Fig2c.jpg" /></p>
<p></p>
<p></p>
<p>Pictures of representative loci were taken on Integrative Genome Viewer (IGV) for SUZ12 and H3K27me3 triplicate samples, showing an overlap between the signal from both proteins, as part of the Polycomb Repressive Complex.</p>
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig2-loci1.jpg" width="100%" /><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig2-loci2.jpg" /><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig2-loci3.jpg" /></p>
<p><strong>Figure 2. IGV snapshots</strong></p>
<p></p>
<p></p>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig3.jpg" /></p>
</div>
<div class="small-8 columns">
<p><strong>Figure 3. Heatmap around the TSS</strong><br /> Heatmap of the CTCF signal around the transcription start sites (TSS) of each gene present in the murine mm10 reference genome.</p>
</div>
</div>
<p></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig4.jpg" /></p>
<p><strong>Figure 4. CTCF motif</strong><br /> Presence of specific transcription factor binding motifs in the regions identified as CTCF peaks. The top3 motifs are corresponding to the CTCF binding motif.</p>
<p></p>
<p></p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig5.jpg" /></p>
</div>
<div class="small-6 columns">
<p><strong>Figure 5. CTCF motif density</strong><br /> Location and density of the CTCF binding motif with respect to the center of the identified CTCF peaks.</p>
</div>
</div>
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'meta_title' => 'iDeal CUT&Tag Kit for Histones for chromatin profiling | Diagenode',
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'meta_description' => 'Rapid chromatin profiling assay, optimized protocol, all reagents provided. Easy sample handling due to ConA magnetic beads. Highly specific CUT&Tag grade antibodies available. ',
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'name' => 'ChIPmentation Kit for Histones',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/chipmentation-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p><b>ChIPmentation</b> is a method that combines <b>chromatin </b><b>immunoprecipiation</b> and <b>tagmentation</b><b>-based library preparation </b>using a fast and robust ChIP-seq protocol for studying <b>protein/DNA interactions</b>. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters. </p>
<p>The <b>ChIPmentation</b><b> Kit for Histones </b>includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The <b>primer indexes </b>for multiplexing are <b>not included</b> in the kit and have to be purchase separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">libraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
</ul>
<p><b>Benefits of the </b><b>ChIPmentation</b><b> system for histone </b><b>ChIP</b><b>-seq</b></p>
<ul>
<li>Easier and faster than classical ChIP-seq</li>
<li>Validated for various histone marks for a standard amount of cells</li>
<li>Generate high quality sequencing data</li>
</ul>
<p>For low input samples (10,000 cells) we recommend the <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation kit for Histones</a>.</p>
<p>For ChIP-seq on transcription factors we recommend the <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal</a> <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">ChIP-seq</a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns"> for transcription </a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">factors</a> + <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG kit for </a><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">ChIPmentation</a></p>',
'label1' => 'Validation',
'info1' => '<p>The Diagenode ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.</p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-1.png" /></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-2.png" /></div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-3.png" /></div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-4.png" /></div>
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<p><small><b>Figure 1: </b><b>ChIPmentation</b> <b>sequencing</b> <b>results</b> <b>obtained</b> <b>from</b> <b>decreasing</b> <b>starting</b> <b>amounts</b><b> of </b><b>cells</b><b>.<br /> </b><br /> Chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.</small></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-5.png" /></p>
<p><small><b>Figure 2: </b><b>ChIPmentation</b><b> sequencing results.</b></small></p>
<p>Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).</p>',
'label2' => 'Additional solutions compatible with ChIPmentation Kit for Histones ',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">iDeal</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells"> Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP</a><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for tagmenteted libraries:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">lilbraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
</ul>
<p>The kit ChIPmentation for Histones is validated on the <a href="https://www.diagenode.com/en/categories/ip-star">IP-Star Compact System </a>and the corresponding protocol is included in the manual.</p>',
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<p style="text-align: justify;">Diagenode’s epigenetic reagents include:</p>
<ul>
<li style="text-align: justify;"><strong>DNA methylation kits and antibodies</strong> - Validated NGS-compatible kits for MeDIP, MBD pull-down, whole genome bisulfite sequencing, and reduced representation bisulfite sequencing. Official provider for the original clone for 5-mC 33D3.</li>
<li style="text-align: justify;"><strong>ChIP and ChIP-seq kits for industry-leading specificity and sensitivity</strong> - MicroChIP/MicroPlex Kit for ChIP-seq with only 10,000 cells and the iDeal ChIP-seq Kits optimized for both transcription factors and histones. Our kits feature full reagents for ChIP-seq including control primers, control antibodies, magnetics beads, and purification reagents.</li>
<li style="text-align: justify;"><strong>Library preparation kits</strong> tailored for your specific requirements. The MicroPlex Library Preparation Kit simplifies library preparation requiring only 3 simple steps and allowing inputs of only 50 pg. </li>
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'description' => '<p>The <strong>Primer indexes for tagmented libraries</strong> are PCR primers targeting the Nextera sequencing adapters, previously incorporated in the libraries by <strong>tagmentation</strong>. Each primer index is baring a <strong>unique index</strong> in order to identify each library before pooling them for sequencing in the same lane. They are compatible with any Nextera-based libraries such as the one generated with ChIPmentation, ATAC-seq or CUT&Tag technologies.</p>
<h3>Features:</h3>
<ul>
<li>Compatibility: <strong>tagmentation-based library</strong> preparation protocols</li>
<li>Flexibility: <strong>Single</strong> and <strong>unique dual indexing</strong> available</li>
<li>Multiplexing capacity: up to <strong>72 samples</strong> (with UDI)</li>
<li>Identification and <strong>filtering of index hopping</strong> – using the UDI</li>
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<p>Get the manual of <a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf" target="_blank" title="Primer indexes for tagmented libraries - Manual">Primer indexes for tagmented libraries</a>.</p>',
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'description' => '<p><span>Alterations in nuclear structure and function are hallmarks of cancer cells. Little is known about these changes in Cancer-Associated Fibroblasts (CAFs), crucial components of the tumor microenvironment. Loss of the androgen receptor (AR) in human dermal fibroblasts (HDFs), which triggers early steps of CAF activation, leads to nuclear membrane changes and micronuclei formation, independent of cellular senescence. Similar changes occur in established CAFs and are reversed by restoring AR activity. AR associates with nuclear lamin A/C, and its loss causes lamin A/C nucleoplasmic redistribution. AR serves as a bridge between lamin A/C and the protein phosphatase PPP1. Loss of AR decreases lamin-PPP1 association and increases lamin A/C phosphorylation at Ser 301, a characteristic of CAFs. Phosphorylated lamin A/C at Ser 301 binds to the regulatory region of CAF effector genes of the myofibroblast subtype. Expression of a lamin A/C Ser301 phosphomimetic mutant alone can transform normal fibroblasts into tumor-promoting CAFs.</span></p>',
'date' => '2024-09-12',
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'doi' => 'https://doi.org/10.1038/s41467-024-52344-z',
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'description' => '<p><span>ATAC-seq has emerged as a rich epigenome profiling technique, and is commonly used to identify Transcription Factors (TFs) underlying given phenomena. A number of methods can be used to identify differentially-active TFs through the accessibility of their DNA-binding motif, however little is known on the best approaches for doing so. Here we benchmark several such methods using a combination of curated datasets with various forms of short-term perturbations on known TFs, as well as semi-simulations. We include both methods specifically designed for this type of data as well as some that can be repurposed for it. We also investigate variations to these methods, and identify three particularly promising approaches (chromVAR-limma with critical adjustments, monaLisa and a combination of GC smooth quantile normalization and multivariate modeling). We further investigate the specific use of nucleosome-free fragments, the combination of top methods, and the impact of technical variation. Finally, we illustrate the use of the top methods on a novel dataset to characterize the impact on DNA accessibility of TRAnscription Factor TArgeting Chimeras (TRAFTAC), which can deplete TFs – in our case NFkB – at the protein level.</span></p>',
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'description' => '<p><span>Translation of seed stored mRNAs is essential to trigger germination. However, when RNAPII re-engages RNA synthesis during the seed-to-seedling transition has remained in question. Combining csRNA-seq, ATAC-seq and smFISH in </span><i>Arabidopsis thaliana</i><span><span> </span>we demonstrate that active transcription initiation is detectable during the entire germination process. Features of non-coding regulation such as dynamic changes in chromatin accessible regions, antisense transcription, as well as bidirectional non-coding promoters are widespread throughout the Arabidopsis genome. We show that sensitivity to exogenous ABSCISIC ACID (ABA) during germination depends on proximal promoter accessibility at ABA-responsive genes. Moreover, we provide genetic validation of the existence of divergent transcription in plants. Our results reveal that active enhancer elements are transcribed producing non-coding enhancer RNAs (eRNAs) as widely documented in metazoans. In sum, this study defining the extent and role of coding and non-coding transcription during key stages of germination expands our understanding of transcriptional mechanisms underlying plant developmental transitions.</span></p>',
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'description' => '<p><span>Sexual reproduction of </span><i>Toxoplasma gondii</i><span>, confined to the felid gut, remains largely uncharted owing to ethical concerns regarding the use of cats as model organisms. Chromatin modifiers dictate the developmental fate of the parasite during its multistage life cycle, but their targeting to stage-specific cistromes is poorly described</span><sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1" title="Farhat, D. C. et al. A MORC-driven transcriptional switch controls Toxoplasma developmental trajectories and sexual commitment. Nat. Microbiol. 5, 570–583 (2020)." href="https://www.nature.com/articles/s41586-023-06821-y#ref-CR1" id="ref-link-section-d277698175e527">1</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2" title="Bougdour, A. et al. Drug inhibition of HDAC3 and epigenetic control of differentiation in Apicomplexa parasites. J. Exp. Med. 206, 953–966 (2009)." href="https://www.nature.com/articles/s41586-023-06821-y#ref-CR2" id="ref-link-section-d277698175e530">2</a></sup><span>. Here we found that the transcription factors AP2XII-1 and AP2XI-2 operate during the tachyzoite stage, a hallmark of acute toxoplasmosis, to silence genes necessary for merozoites, a developmental stage critical for subsequent sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a marked change in the transcriptional program, promoting a full transition from tachyzoites to merozoites. These in vitro-cultured pre-gametes have unique protein markers and undergo typical asexual endopolygenic division cycles. In tachyzoites, AP2XII-1 and AP2XI-2 bind DNA as heterodimers at merozoite promoters and recruit MORC and HDAC3 (ref. </span><sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1" title="Farhat, D. C. et al. A MORC-driven transcriptional switch controls Toxoplasma developmental trajectories and sexual commitment. Nat. Microbiol. 5, 570–583 (2020)." href="https://www.nature.com/articles/s41586-023-06821-y#ref-CR1" id="ref-link-section-d277698175e534">1</a></sup><span>), thereby limiting chromatin accessibility and transcription. Consequently, the commitment to merogony stems from a profound epigenetic rewiring orchestrated by AP2XII-1 and AP2XI-2. Successful production of merozoites in vitro paves the way for future studies on<span> </span></span><i>Toxoplasma</i><span><span> </span>sexual development without the need for cat infections and holds promise for the development of therapies to prevent parasite transmission.</span></p>',
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'description' => '<p><span>Universal Minicircle Sequence binding proteins (UMSBPs) are CCHC-type zinc-finger proteins that bind the single-stranded G-rich UMS sequence, conserved at the replication origins of minicircles in the kinetoplast DNA, the mitochondrial genome of kinetoplastids. Trypanosoma brucei UMSBP2 has been recently shown to colocalize with telomeres and to play an essential role in chromosome end protection. Here we report that TbUMSBP2 decondenses in vitro DNA molecules, which were condensed by core histones H2B, H4 or linker histone H1. DNA decondensation is mediated via protein-protein interactions between TbUMSBP2 and these histones, independently of its previously described DNA binding activity. Silencing of the TbUMSBP2 gene resulted in a significant decrease in the disassembly of nucleosomes in T. brucei chromatin, a phenotype that could be reverted, by supplementing the knockdown cells with TbUMSBP2. Transcriptome analysis revealed that silencing of TbUMSBP2 affects the expression of multiple genes in T. brucei, with a most significant effect on the upregulation of the subtelomeric variant surface glycoproteins (VSG) genes, which mediate the antigenic variation in African trypanosomes. These observations suggest that UMSBP2 is a chromatin remodeling protein that functions in the regulation of gene expression and plays a role in the control of antigenic variation in T. brucei.</span></p>',
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<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p>The 8 UDI for tagmented libraries includes 8 primer pairs for unique dual-indexing allowing the multiplexing of up to 8<b> samples </b>for sequencing on Illumina platforms. This set of indexes is designed to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones (Cat. No. C01011011)</a>, <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones (Cat. No. C01011009)</a>, <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation (Cat. No. C01011030)</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit (Cat. No. C01080002)</a> but it is also compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <b>ATAC-seq</b> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>',
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<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
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<p><b>ChIPmentation</b> is a method that combines <b>chromatin </b><b>immunoprecipiation</b> and <b>tagmentation</b><b>-based library preparation </b>using a fast and robust ChIP-seq protocol for studying <b>protein/DNA interactions</b>. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters. </p>
<p>The <b>ChIPmentation</b><b> Kit for Histones </b>includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The <b>primer indexes </b>for multiplexing are <b>not included</b> in the kit and have to be purchase separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">libraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
</ul>
<p><b>Benefits of the </b><b>ChIPmentation</b><b> system for histone </b><b>ChIP</b><b>-seq</b></p>
<ul>
<li>Easier and faster than classical ChIP-seq</li>
<li>Validated for various histone marks for a standard amount of cells</li>
<li>Generate high quality sequencing data</li>
</ul>
<p>For low input samples (10,000 cells) we recommend the <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation kit for Histones</a>.</p>
<p>For ChIP-seq on transcription factors we recommend the <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal</a> <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">ChIP-seq</a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns"> for transcription </a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">factors</a> + <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG kit for </a><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">ChIPmentation</a></p>',
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'info1' => '<p>The Diagenode ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.</p>
<p></p>
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<p><small><b>Figure 1: </b><b>ChIPmentation</b> <b>sequencing</b> <b>results</b> <b>obtained</b> <b>from</b> <b>decreasing</b> <b>starting</b> <b>amounts</b><b> of </b><b>cells</b><b>.<br /> </b><br /> Chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.</small></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-5.png" /></p>
<p><small><b>Figure 2: </b><b>ChIPmentation</b><b> sequencing results.</b></small></p>
<p>Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).</p>',
'label2' => 'Additional solutions compatible with ChIPmentation Kit for Histones ',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">iDeal</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells"> Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP</a><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for tagmenteted libraries:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">lilbraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
</ul>
<p>The kit ChIPmentation for Histones is validated on the <a href="https://www.diagenode.com/en/categories/ip-star">IP-Star Compact System </a>and the corresponding protocol is included in the manual.</p>',
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<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
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'description' => '<p><span>Universal Minicircle Sequence binding proteins (UMSBPs) are CCHC-type zinc-finger proteins that bind the single-stranded G-rich UMS sequence, conserved at the replication origins of minicircles in the kinetoplast DNA, the mitochondrial genome of kinetoplastids. Trypanosoma brucei UMSBP2 has been recently shown to colocalize with telomeres and to play an essential role in chromosome end protection. Here we report that TbUMSBP2 decondenses in vitro DNA molecules, which were condensed by core histones H2B, H4 or linker histone H1. DNA decondensation is mediated via protein-protein interactions between TbUMSBP2 and these histones, independently of its previously described DNA binding activity. Silencing of the TbUMSBP2 gene resulted in a significant decrease in the disassembly of nucleosomes in T. brucei chromatin, a phenotype that could be reverted, by supplementing the knockdown cells with TbUMSBP2. Transcriptome analysis revealed that silencing of TbUMSBP2 affects the expression of multiple genes in T. brucei, with a most significant effect on the upregulation of the subtelomeric variant surface glycoproteins (VSG) genes, which mediate the antigenic variation in African trypanosomes. These observations suggest that UMSBP2 is a chromatin remodeling protein that functions in the regulation of gene expression and plays a role in the control of antigenic variation in T. brucei.</span></p>',
'date' => '2023-05-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37207337',
'doi' => '10.1093/nar/gkad402',
'modified' => '2023-06-15 08:54:17',
'created' => '2023-06-13 21:11:31',
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<p>3 sets of UDI for tagmented libraries are available:</p>
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<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
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<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
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<div class="extra-spaced">
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'info1' => '<p>ChIPmentation is based on tagmentation that allows library preparation to be integrated during the ChIP itself using transposase and sequencing-compatible adaptors. Our new improved µChIPmentation protocol combines 3 features for guaranteeing high quality sequencing data on small sample inputs 1) optimized chromatin shearing preparation protocol, 2) reduced number of steps, 3) reduced number of sample transfer from tube to tube - only 3 tubes per sample for the whole process, from cell fixation to purified libraries.</p>
<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"><i class="fa fa-caret-right"></i> Workflow of µChIPmentation for Histones - Read more</a>
<div id="workflow" class="content">
<div class="extra-spaced"><center><img src="https://www.diagenode.com/img/product/kits/workflow-micro-chipmentation.png" /></center></div>
</div>
</li>
</ul>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns"><strong>A.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1A.png" /></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-5 large-5 columns" columns=""><strong>B.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1B.png" /></div>
<div class="small-12 medium-7 large-7 columns">
<p style="text-align: left;"><strong>Figure 1. Comparison between ChIPmentation and µChIPmentation </strong><br />ChIPmentation: chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011000) and diluted chromatin from 500.000 cells was used for the immunoprecipitation. µChIPmentation: chromatin preparation and immunoprecipitation have been performed on 10.000 K562 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032). The Diagenode antibody targeting H3K27me3 (Cat. No. C15410195) was used.<br /> A. Distribution of the ChIPmentation and µChIPmentation readsets in a representative region of the genome (in duplicates). B. Comparison of the top 40% peaks from µChIPmentation (10.000 cells) with dataset generated with ChIPmentation (500.000 cells).</p>
</div>
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<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig2.png" /></div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 2. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
</div>
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</div>
<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig3.png" /></div>
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<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 3. Integrative genomics viewer (IGV) visualization of from ChIP-seq experiments using H3K27me3 antibody (Diagenode, Cat. No. <span>C15410195</span>) and 10.000 cells of K562 cells per immunoprecipitation.<br /></strong>Cells were FACS-sorted and ChIP has been performed accordingly to µChIPmentation protocol. Batch chromatin preparation followed by immunoprecipitation has been used per comparison as indicated.</p>
</div>
</div>
</div>',
'label2' => 'Additional solutions for µChIPmentation Kit for Histones',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit - High SDS</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for multiplexing:</p>
<ul class="no-bullet">
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries" target="_blank">24 SI for Tagmented libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries" target="_blank">8 SI for Tagmented libraries Cat. No. C01011033</a></li>
<li><em></em><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for Tagmented libraries, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
<li></li>
</ul>',
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<div align="center"><video width="400" height="250" autoplay="autoplay" muted="" loop="loop" controls="controls" src="https://www.diagenode.com/videos/chipmentation-dgo.mp4" frameborder="0" allowfullscreen="allowfullscreen"></video></div>
<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<p>Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fragments in one step. In our ChIPmentation technology we combine chromatin immunoprecipitation and tagmentation in one streamlined workflow where the tagmentation step occurs directly on chromatin. The <strong>TAG Kit for ChIPmentation</strong> has been developed for researchers who would like to perform ChIPmentation using their own validated ChIP protocol. Our TAG Kit for ChIPmentation includes reagents for <strong>tagmentation-based library preparation</strong> and is compatible with any ChIP protocol based on magnetic beads. The <strong>primer</strong> <strong>indexes for multiplexing</strong> must be <strong>purchased separately</strong> and are available as a reference:<br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank"><br /></a></p>',
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'info1' => '<h3>Comparing to classical ChIP-seq, ChIPmentation enables:</h3>
<ul>
<li>An easier protocol</li>
<li>Faster time to results</li>
<li>Flexibility – the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows you to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
</ul>
<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
<p><small>*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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'name' => 'ATAC-seq kit',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/atacseq-kit-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p><strong>ATAC-seq</strong>, Assay for<span> </span><strong>T</strong>ransposase-<strong>A</strong>ccessible<span> </span><strong>C</strong>hromatin, followed by next generation sequencing, is a key technology for genome-wide mapping of accessible chromatin. The technology is based on the use of the<span> </span><strong>transposase Tn5</strong><span> </span>which cuts exposed open chromatin and simultaneously ligates adapters for subsequent amplification and sequencing. ATAC-seq methods allow you to:</p>
<ul>
<li> Gain insight into gene regulation and understand open chromatin signatures</li>
<li> Determine nucleosome positions at single nucleotide resolution</li>
<li> Uncover transcription factor (TF) occupancy</li>
</ul>
</div>
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<p>Diagenode’s<span> </span><b>ATAC-</b><b>seq</b><b><span> </span>kit<span> </span></b>is based on a highly validated protocol optimized for<span> </span><b>50,000<span> </span></b><b>cells</b><b><span> </span>per<span> </span></b><b>reaction</b>. The kit includes the reagents for cell lysis and nuclei extraction, tagmentation and DNA purification as well as for library amplification. The <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries">primer indexes for multiplexing</a> are not included in the kit and must be purchased separately.</p>
<h4><span style="font-weight: 400;">ATAC-seq kit features:</span></h4>
<ul>
<li><b>Cell<span> </span></b><b>requirement</b><b>:<span> </span></b><b>50,000<span> </span></b><b>cells /<span> </span></b><b>rxn</b></li>
<li><b>Robust protocol<span> </span></b>with<span> </span><b>high reproducibility<span> </span></b>between replicates and repetitive experiments</li>
<li><strong>Easy</strong><span> </span>and<span> </span><b>efficient DNA capture<span> </span></b>after the tagmentation reaction using Diagenode`s MicroChIP DiaPure columns (included)</li>
<li>Additional qPCR step to determine the number of cycles needed for library amplification: </li>
<ul type="”square”">
<li><b>Avoids<span> </span></b><b>over-amplification</b></li>
<li>Allows adaptation/flexibility for<span> </span><b>more challenging samples<span> </span></b>to succeed with library prep.</li>
<li>Gives<span> </span><strong>early indication</strong><span> </span>if the experiment does not work (no qPCR amplification)</li>
</ul>
</ul>
<p>Looking for ATAC-seq on tissue? Please, go to: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080006">ATAC-seq package for tissue</a></p>',
'label1' => 'Method overview',
'info1' => '<p><strong>ATAC-seq</strong>, <strong>A</strong>ssay for <strong>T</strong>ransposase-<strong>A</strong>ccessible <strong>C</strong>hromatin, followed by next generation sequencing, is a key technology to easily identify the <strong>open regions of the chromatin.</strong> The protocol consists of <strong>3 steps</strong>: <strong>nuclei preparation</strong>, <strong>tagmentation</strong> and <strong>library amplification</strong>. First, the cells undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq.png" alt="ATAC-seq kit workflow" width="600px" caption="false" /></p>',
'label2' => 'Example of results',
'info2' => '<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig1.png" alt="library prepared with the Diagenode ATAC-seq kit " width="500px" caption="false" /></p>
<p><strong>Figure 1.</strong>Representative Bioanalyzer profile of an ATAC-seq library prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells.</p>
<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig2.png" alt="Diagenode ATAC-seq kit " caption="false" width="951" height="148" /></p>
<p><strong>Figure 2.</strong> Main ATAC-seq alignment and peak calling statistics of 3 replicates prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells. (Mapping efficiency: Percentage of non-mitochondrial reads that mapped to the reference genome. Uniquely mapped ratio: Proportion of mapped reads that map to only one location on the reference genome (hg19). Peaks: Number of peaks (open chromatin regions) identified by MACS2 for each sample. FRiP - Fraction of reads in peaks: Percentage of reads in peaks, with respect to the number of uniquely mapped reads. Sequencing was realized in paired-end mode 50 base pairs (PE50) on an Illumina NovaSeq6000.)</p>
<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig3a.png" alt="Assay for Transposase-Accessible Chromatin" width="500px" caption="false" /></p>
<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig3b.png" alt="Assay for Transposase-Accessible Chromatin" width="500px" caption="false" /></p>
<p><strong>Figure 3</strong> Sequencing profiles of ATAC-seq library (3 replicates) prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells.</p>
<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig4.png" alt=" open chromatin regions" caption="false" width="383" height="739" /></p>
<p><strong>Figure 4. </strong><br /> Heatmap around TSS of three ATAC-seq replicates prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells.</p>',
'label3' => 'Additional solutions for ATAC-seq kit',
'info3' => '<p><a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries">Primer indexes for tagmented libraries</a></p>
<p>Magnetic rack:<span> </span><a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag</a><a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit"><span> </span>0.2 ml – Cat. No. B04000001</a></p>
<p>Additional supplies (included in the kit and available separately):</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase</a><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30"><span> </span>(Tn5 transposase)<span> </span></a><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">loaded</a><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">, Cat. No. C01070012</a></li>
<li><a href="https://www.diagenode.com/en/p/tagmentation-buffer-2x">Tagmentation</a><a href="https://www.diagenode.com/en/p/tagmentation-buffer-2x"><span> </span>Buffer (2x), Cat. No. C01019043</a></li>
<li><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP</a><span> </span><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">DiaPure</a><span> </span><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">columns</a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">, Cat. No. C03040001</a></li>
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'meta_description' => 'Diagenode’s ATAC-seq kit provides a robust protocol for assessing genome-wide chromatin accessibility',
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'name' => 'iDeal CUT&Tag kit for Histones <br /> Compatible with histones and some non-histone proteins',
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<p><strong>CUT&Tag-sequencing</strong> (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins.</p>
<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
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<p>The Diagenode’s <strong>iDeal CUT&Tag kit for Histones</strong> provides an optimized protocol for a rapid chromatin profiling on <strong>histone marks</strong> and some <strong>non-histone proteins</strong>. The protocol is optimized for native cells (<strong>10,000-300,000</strong> cells per reaction) and can be completed within 1.5 days. The kit includes all reagents for cell processing, including CoA beads, pA-Tn5 and the DNA purification module. The antibodies (secondary antibodies, control antibodies) as well as primer indexes for multiplexing must be purchased separately.</p>
<p><strong>For a complete CUT&Tag protocol the following items must be purchased:</strong></p>
<ul>
<li><strong>iDeal CUT&Tag kit for Histones</strong> – including all reagents for CUT&Tag workflow (buffers, pA-Tn5, CoA beads, DNA purification)</li>
<li><strong>Antibody package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a></strong> or <strong><a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></strong> - including the secondary antibody, positive and negative control antibodies and primers</li>
<li><strong><a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries">Primer indexes for tagmented libraries</a></strong> – for multiplexing up to 72 samples</li>
</ul>
<h3>iDeal CUT&Tag Kit features:</h3>
<ul>
<li><strong>Rapid</strong> and <strong>easy </strong>chromatin profiling assay for <strong>histones </strong>and<strong> some non-histone proteins</strong></li>
<ul style="margin-bottom: 0;">
<li>No chromatin preparation</li>
<li>Easy sample handling due to ConA magnetic beads</li>
<li>Integrated library prep</li>
</ul>
<li><strong>Low cell number</strong>: 10,000-300,000 cells</li>
<li><strong>Accurate amplification</strong> due to intermediate quantification step</li>
<li><strong>High resolution</strong> and <strong>sensitivity</strong></li>
<li><strong>Lower sequencing depth</strong></li>
</ul>
<p>The quality of antibody used in CUT&Tag is one of the crucial factors for assay success. The antibodies with confirmed high specificity will target only the protein of interest, enabling real results. Check out our selection of <strong><a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">antibodies validated in CUT&Tag</a>.</strong></p>
<p>Looking for a standalone pA-Tn5? <a href="https://www.diagenode.com/en/products/view/3064">Read more</a>.</p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Method overview',
'info1' => '<p>The iDeal CUT&Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode’s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode’s Primer Indexes for tagmented libraries.</p>
<p></p>
<p><img alt=" pA-Tn5 Antibody package for CUT & Tag" src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></p>',
'label2' => 'Examples of results - histone marks',
'info2' => '<p>Successful CUT&Tag results showing a low background with high region-specific enrichment are presented below. Chromatin profiling has been performed on 50,000 K562 cells, using the Diagenode’s iDeal CUT&Tag kit for Histones (Cat. No. C01070020), the Antibody Package for CUT&Tag anti rabbit (Cat. No. C01070022), the 24 UDI for Tagmented Libraries (Cat.No. C01011034) and H3K4me3 (Cat. No. C15410003), H3K27me3 (Cat. No. C15410069) or H3H9me3 antibodies (Cat. No., C15410193) as indicated. The libraries were sequenced on Illumina’s NovaSeq6000 in 2x50 bp mode.</p>
<p><br /> <img alt="CUT&Tag-sequencing" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig1a.png" /> <img alt="CUT&Tag kit for Histones" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig1b.png" /> <img alt="Cleavage Under Targets and Tagmentation" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig1c.png" /></p>
<p><strong>Figure 1.</strong> Typical library profiles (Agilent Fragment traces) generated by the iDeal CUT&Tag protocol using 50,000 K562 cells and H3K27me3 (top), H3K4me4 (middle) primary antibodies and IgG control (bottom). Sharp peak at around 40 bp is an excess of <strong>free oligonucleotide used for pA-Tn5 loading</strong>.</p>
<p></p>
<p><br /><br /></p>
<div class="row">
<div class="small-4 columns">
<p><img alt="iDeal CUT&Tag kit for Histones" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig2.png" /></p>
</div>
<div class="small-8 columns">
<p><strong>Figure 2.</strong> Enrichments at TSS of the CUT&Tag libraries. The heatmap shows the enrichment around 3kb upstream and downstream of the TSS for H3K4me3. H3K4me3 as an active chromatin mark is associated with active promoters shows a narrow enrichment pattern.</p>
</div>
</div>
<p><br /><br /></p>
<p><br /> <img alt="iDeal CUT&Tag experiments of K562 cells " src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig3.png" /></p>
<p><strong>Figure 3.</strong> Sequencing profiles of the CUT&Tag libraries. Integrative genomics viewer (IGV) visualization of CUT&Tag experiments using 50,000 of K562 cells and H3K4me3 (blue), H3K27me3 (red) or H3K9me3 (green).</p>
<p><br /><br /></p>
<p><br /> <img alt="CUT&Tag experiments using 10,000 K562 cells" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig4.png" /></p>
<p><strong>Figure 4.</strong> Sequencing profiles of the CUT&Tag libraries. Integrative genomics viewer (IGV) visualization of CUT&Tag experiments using 50,000 (blue) or 10,000 (red)) of K562 cells and H3K27me3 antibody.</p>',
'label3' => 'Examples of results - transcription factors',
'info3' => '<p>Diagenode's<strong><span> iDeal CUT&Tag Kit for Histones</span></strong><span>, </span>developed for an efficient chromatin profiling on histone marks, can be used for profiling of <span>some transcription factors and co-factors using a mild fixation as described in the manual. </span></p>
<p><strong><span>Successful CUT&Tag results using CTCF and Suz12 antibodies and iDeal CUT&Tag Kit for Histones. </span></strong></p>
<p></p>
<p><span>Chromatin profiling of <strong>Suz12</strong> has been performed on 50,000 of fixed Mouse ES-E14TG2a cells (kindly provided by Luciano Di Croce, CRG, Spain) accordingly to the protocol of Diagenode’s iDeal CUT&Tag kit for Histones (Cat. No. C01070020). Antibody Package for CUT&Tag anti rabbit (Cat. No. C01070022) and the 24 UDI for Tagmented Libraries (Cat. No. C01011034) were used. The libraries were sized using Fragment Analyzer (Agilent) (triplicates, Figure 1, top). Relative enrichment has been confirmed by qPCR using know positive (T_Bra) and negative (Actb) loci (Figure 1, bottom). Libraries were sequenced on Illumina’s NovaSeq6000 in 2x50 bp mode.</span></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig1a.jpg" /><br /> <img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig1b.jpg" /></p>
<p><strong>Figure 1.</strong> Typical library profiles (top) and relative enrichment (bottom) generated by the iDeal CUT&Tag protocol using 50,000 ES-E14TG2a cells and Suz12 Antibody and IgG control (bottom). Sharp peak at around 40 bp is an excess of free oligonucleotide used for pA-Tn5 loading which does not interfere with the sequencing.</p>
<p></p>
<p>Pictures of representative loci were taken on Integrative Genome Viewer (IGV) for CTCF demonstrating the presence of high signal at promoter regions.</p>
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-Fig2a.jpg" /><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-Fig2b.jpg" /> <img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-Fig2c.jpg" /></p>
<p></p>
<p></p>
<p>Pictures of representative loci were taken on Integrative Genome Viewer (IGV) for SUZ12 and H3K27me3 triplicate samples, showing an overlap between the signal from both proteins, as part of the Polycomb Repressive Complex.</p>
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig2-loci1.jpg" width="100%" /><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig2-loci2.jpg" /><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig2-loci3.jpg" /></p>
<p><strong>Figure 2. IGV snapshots</strong></p>
<p></p>
<p></p>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig3.jpg" /></p>
</div>
<div class="small-8 columns">
<p><strong>Figure 3. Heatmap around the TSS</strong><br /> Heatmap of the CTCF signal around the transcription start sites (TSS) of each gene present in the murine mm10 reference genome.</p>
</div>
</div>
<p></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig4.jpg" /></p>
<p><strong>Figure 4. CTCF motif</strong><br /> Presence of specific transcription factor binding motifs in the regions identified as CTCF peaks. The top3 motifs are corresponding to the CTCF binding motif.</p>
<p></p>
<p></p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig5.jpg" /></p>
</div>
<div class="small-6 columns">
<p><strong>Figure 5. CTCF motif density</strong><br /> Location and density of the CTCF binding motif with respect to the center of the identified CTCF peaks.</p>
</div>
</div>
<p>
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'meta_title' => 'iDeal CUT&Tag Kit for Histones for chromatin profiling | Diagenode',
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'meta_description' => 'Rapid chromatin profiling assay, optimized protocol, all reagents provided. Easy sample handling due to ConA magnetic beads. Highly specific CUT&Tag grade antibodies available. ',
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'name' => 'ChIPmentation Kit for Histones',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/chipmentation-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p><b>ChIPmentation</b> is a method that combines <b>chromatin </b><b>immunoprecipiation</b> and <b>tagmentation</b><b>-based library preparation </b>using a fast and robust ChIP-seq protocol for studying <b>protein/DNA interactions</b>. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters. </p>
<p>The <b>ChIPmentation</b><b> Kit for Histones </b>includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The <b>primer indexes </b>for multiplexing are <b>not included</b> in the kit and have to be purchase separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">libraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
</ul>
<p><b>Benefits of the </b><b>ChIPmentation</b><b> system for histone </b><b>ChIP</b><b>-seq</b></p>
<ul>
<li>Easier and faster than classical ChIP-seq</li>
<li>Validated for various histone marks for a standard amount of cells</li>
<li>Generate high quality sequencing data</li>
</ul>
<p>For low input samples (10,000 cells) we recommend the <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation kit for Histones</a>.</p>
<p>For ChIP-seq on transcription factors we recommend the <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal</a> <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">ChIP-seq</a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns"> for transcription </a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">factors</a> + <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG kit for </a><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">ChIPmentation</a></p>',
'label1' => 'Validation',
'info1' => '<p>The Diagenode ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.</p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-1.png" /></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-2.png" /></div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-3.png" /></div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-4.png" /></div>
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<p><small><b>Figure 1: </b><b>ChIPmentation</b> <b>sequencing</b> <b>results</b> <b>obtained</b> <b>from</b> <b>decreasing</b> <b>starting</b> <b>amounts</b><b> of </b><b>cells</b><b>.<br /> </b><br /> Chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.</small></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-5.png" /></p>
<p><small><b>Figure 2: </b><b>ChIPmentation</b><b> sequencing results.</b></small></p>
<p>Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).</p>',
'label2' => 'Additional solutions compatible with ChIPmentation Kit for Histones ',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">iDeal</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells"> Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP</a><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for tagmenteted libraries:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">lilbraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
</ul>
<p>The kit ChIPmentation for Histones is validated on the <a href="https://www.diagenode.com/en/categories/ip-star">IP-Star Compact System </a>and the corresponding protocol is included in the manual.</p>',
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<p style="text-align: justify;">Diagenode’s epigenetic reagents include:</p>
<ul>
<li style="text-align: justify;"><strong>DNA methylation kits and antibodies</strong> - Validated NGS-compatible kits for MeDIP, MBD pull-down, whole genome bisulfite sequencing, and reduced representation bisulfite sequencing. Official provider for the original clone for 5-mC 33D3.</li>
<li style="text-align: justify;"><strong>ChIP and ChIP-seq kits for industry-leading specificity and sensitivity</strong> - MicroChIP/MicroPlex Kit for ChIP-seq with only 10,000 cells and the iDeal ChIP-seq Kits optimized for both transcription factors and histones. Our kits feature full reagents for ChIP-seq including control primers, control antibodies, magnetics beads, and purification reagents.</li>
<li style="text-align: justify;"><strong>Library preparation kits</strong> tailored for your specific requirements. The MicroPlex Library Preparation Kit simplifies library preparation requiring only 3 simple steps and allowing inputs of only 50 pg. </li>
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'description' => '<p>The <strong>Primer indexes for tagmented libraries</strong> are PCR primers targeting the Nextera sequencing adapters, previously incorporated in the libraries by <strong>tagmentation</strong>. Each primer index is baring a <strong>unique index</strong> in order to identify each library before pooling them for sequencing in the same lane. They are compatible with any Nextera-based libraries such as the one generated with ChIPmentation, ATAC-seq or CUT&Tag technologies.</p>
<h3>Features:</h3>
<ul>
<li>Compatibility: <strong>tagmentation-based library</strong> preparation protocols</li>
<li>Flexibility: <strong>Single</strong> and <strong>unique dual indexing</strong> available</li>
<li>Multiplexing capacity: up to <strong>72 samples</strong> (with UDI)</li>
<li>Identification and <strong>filtering of index hopping</strong> – using the UDI</li>
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<p>Get the manual of <a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf" target="_blank" title="Primer indexes for tagmented libraries - Manual">Primer indexes for tagmented libraries</a>.</p>',
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<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
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<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
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<p><b>ChIPmentation</b> is a method that combines <b>chromatin </b><b>immunoprecipiation</b> and <b>tagmentation</b><b>-based library preparation </b>using a fast and robust ChIP-seq protocol for studying <b>protein/DNA interactions</b>. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters. </p>
<p>The <b>ChIPmentation</b><b> Kit for Histones </b>includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The <b>primer indexes </b>for multiplexing are <b>not included</b> in the kit and have to be purchase separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">libraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
</ul>
<p><b>Benefits of the </b><b>ChIPmentation</b><b> system for histone </b><b>ChIP</b><b>-seq</b></p>
<ul>
<li>Easier and faster than classical ChIP-seq</li>
<li>Validated for various histone marks for a standard amount of cells</li>
<li>Generate high quality sequencing data</li>
</ul>
<p>For low input samples (10,000 cells) we recommend the <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation kit for Histones</a>.</p>
<p>For ChIP-seq on transcription factors we recommend the <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal</a> <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">ChIP-seq</a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns"> for transcription </a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">factors</a> + <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG kit for </a><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">ChIPmentation</a></p>',
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'info1' => '<p>The Diagenode ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.</p>
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<p><small><b>Figure 1: </b><b>ChIPmentation</b> <b>sequencing</b> <b>results</b> <b>obtained</b> <b>from</b> <b>decreasing</b> <b>starting</b> <b>amounts</b><b> of </b><b>cells</b><b>.<br /> </b><br /> Chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.</small></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-5.png" /></p>
<p><small><b>Figure 2: </b><b>ChIPmentation</b><b> sequencing results.</b></small></p>
<p>Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).</p>',
'label2' => 'Additional solutions compatible with ChIPmentation Kit for Histones ',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">iDeal</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells"> Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP</a><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for tagmenteted libraries:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">lilbraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
</ul>
<p>The kit ChIPmentation for Histones is validated on the <a href="https://www.diagenode.com/en/categories/ip-star">IP-Star Compact System </a>and the corresponding protocol is included in the manual.</p>',
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<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
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'name' => 'UMSBP2 is chromatin remodeler that functions in regulation of geneexpression and suppression of antigenic variation in trypanosomes.',
'authors' => 'Soni A. et al.',
'description' => '<p><span>Universal Minicircle Sequence binding proteins (UMSBPs) are CCHC-type zinc-finger proteins that bind the single-stranded G-rich UMS sequence, conserved at the replication origins of minicircles in the kinetoplast DNA, the mitochondrial genome of kinetoplastids. Trypanosoma brucei UMSBP2 has been recently shown to colocalize with telomeres and to play an essential role in chromosome end protection. Here we report that TbUMSBP2 decondenses in vitro DNA molecules, which were condensed by core histones H2B, H4 or linker histone H1. DNA decondensation is mediated via protein-protein interactions between TbUMSBP2 and these histones, independently of its previously described DNA binding activity. Silencing of the TbUMSBP2 gene resulted in a significant decrease in the disassembly of nucleosomes in T. brucei chromatin, a phenotype that could be reverted, by supplementing the knockdown cells with TbUMSBP2. Transcriptome analysis revealed that silencing of TbUMSBP2 affects the expression of multiple genes in T. brucei, with a most significant effect on the upregulation of the subtelomeric variant surface glycoproteins (VSG) genes, which mediate the antigenic variation in African trypanosomes. These observations suggest that UMSBP2 is a chromatin remodeling protein that functions in the regulation of gene expression and plays a role in the control of antigenic variation in T. brucei.</span></p>',
'date' => '2023-05-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37207337',
'doi' => '10.1093/nar/gkad402',
'modified' => '2023-06-15 08:54:17',
'created' => '2023-06-13 21:11:31',
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<p>3 sets of UDI for tagmented libraries are available:</p>
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<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
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<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
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<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
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<div class="extra-spaced">
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'info1' => '<p>ChIPmentation is based on tagmentation that allows library preparation to be integrated during the ChIP itself using transposase and sequencing-compatible adaptors. Our new improved µChIPmentation protocol combines 3 features for guaranteeing high quality sequencing data on small sample inputs 1) optimized chromatin shearing preparation protocol, 2) reduced number of steps, 3) reduced number of sample transfer from tube to tube - only 3 tubes per sample for the whole process, from cell fixation to purified libraries.</p>
<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"><i class="fa fa-caret-right"></i> Workflow of µChIPmentation for Histones - Read more</a>
<div id="workflow" class="content">
<div class="extra-spaced"><center><img src="https://www.diagenode.com/img/product/kits/workflow-micro-chipmentation.png" /></center></div>
</div>
</li>
</ul>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns"><strong>A.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1A.png" /></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-5 large-5 columns" columns=""><strong>B.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1B.png" /></div>
<div class="small-12 medium-7 large-7 columns">
<p style="text-align: left;"><strong>Figure 1. Comparison between ChIPmentation and µChIPmentation </strong><br />ChIPmentation: chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011000) and diluted chromatin from 500.000 cells was used for the immunoprecipitation. µChIPmentation: chromatin preparation and immunoprecipitation have been performed on 10.000 K562 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032). The Diagenode antibody targeting H3K27me3 (Cat. No. C15410195) was used.<br /> A. Distribution of the ChIPmentation and µChIPmentation readsets in a representative region of the genome (in duplicates). B. Comparison of the top 40% peaks from µChIPmentation (10.000 cells) with dataset generated with ChIPmentation (500.000 cells).</p>
</div>
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<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig2.png" /></div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 2. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
</div>
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</div>
<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig3.png" /></div>
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<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 3. Integrative genomics viewer (IGV) visualization of from ChIP-seq experiments using H3K27me3 antibody (Diagenode, Cat. No. <span>C15410195</span>) and 10.000 cells of K562 cells per immunoprecipitation.<br /></strong>Cells were FACS-sorted and ChIP has been performed accordingly to µChIPmentation protocol. Batch chromatin preparation followed by immunoprecipitation has been used per comparison as indicated.</p>
</div>
</div>
</div>',
'label2' => 'Additional solutions for µChIPmentation Kit for Histones',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit - High SDS</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for multiplexing:</p>
<ul class="no-bullet">
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries" target="_blank">24 SI for Tagmented libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries" target="_blank">8 SI for Tagmented libraries Cat. No. C01011033</a></li>
<li><em></em><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for Tagmented libraries, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
<li></li>
</ul>',
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<div align="center"><video width="400" height="250" autoplay="autoplay" muted="" loop="loop" controls="controls" src="https://www.diagenode.com/videos/chipmentation-dgo.mp4" frameborder="0" allowfullscreen="allowfullscreen"></video></div>
<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<p>Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fragments in one step. In our ChIPmentation technology we combine chromatin immunoprecipitation and tagmentation in one streamlined workflow where the tagmentation step occurs directly on chromatin. The <strong>TAG Kit for ChIPmentation</strong> has been developed for researchers who would like to perform ChIPmentation using their own validated ChIP protocol. Our TAG Kit for ChIPmentation includes reagents for <strong>tagmentation-based library preparation</strong> and is compatible with any ChIP protocol based on magnetic beads. The <strong>primer</strong> <strong>indexes for multiplexing</strong> must be <strong>purchased separately</strong> and are available as a reference:<br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank"><br /></a></p>',
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'info1' => '<h3>Comparing to classical ChIP-seq, ChIPmentation enables:</h3>
<ul>
<li>An easier protocol</li>
<li>Faster time to results</li>
<li>Flexibility – the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows you to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
</ul>
<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
<p><small>*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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'name' => 'ATAC-seq kit',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/atacseq-kit-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p><strong>ATAC-seq</strong>, Assay for<span> </span><strong>T</strong>ransposase-<strong>A</strong>ccessible<span> </span><strong>C</strong>hromatin, followed by next generation sequencing, is a key technology for genome-wide mapping of accessible chromatin. The technology is based on the use of the<span> </span><strong>transposase Tn5</strong><span> </span>which cuts exposed open chromatin and simultaneously ligates adapters for subsequent amplification and sequencing. ATAC-seq methods allow you to:</p>
<ul>
<li> Gain insight into gene regulation and understand open chromatin signatures</li>
<li> Determine nucleosome positions at single nucleotide resolution</li>
<li> Uncover transcription factor (TF) occupancy</li>
</ul>
</div>
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<p>Diagenode’s<span> </span><b>ATAC-</b><b>seq</b><b><span> </span>kit<span> </span></b>is based on a highly validated protocol optimized for<span> </span><b>50,000<span> </span></b><b>cells</b><b><span> </span>per<span> </span></b><b>reaction</b>. The kit includes the reagents for cell lysis and nuclei extraction, tagmentation and DNA purification as well as for library amplification. The <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries">primer indexes for multiplexing</a> are not included in the kit and must be purchased separately.</p>
<h4><span style="font-weight: 400;">ATAC-seq kit features:</span></h4>
<ul>
<li><b>Cell<span> </span></b><b>requirement</b><b>:<span> </span></b><b>50,000<span> </span></b><b>cells /<span> </span></b><b>rxn</b></li>
<li><b>Robust protocol<span> </span></b>with<span> </span><b>high reproducibility<span> </span></b>between replicates and repetitive experiments</li>
<li><strong>Easy</strong><span> </span>and<span> </span><b>efficient DNA capture<span> </span></b>after the tagmentation reaction using Diagenode`s MicroChIP DiaPure columns (included)</li>
<li>Additional qPCR step to determine the number of cycles needed for library amplification: </li>
<ul type="”square”">
<li><b>Avoids<span> </span></b><b>over-amplification</b></li>
<li>Allows adaptation/flexibility for<span> </span><b>more challenging samples<span> </span></b>to succeed with library prep.</li>
<li>Gives<span> </span><strong>early indication</strong><span> </span>if the experiment does not work (no qPCR amplification)</li>
</ul>
</ul>
<p>Looking for ATAC-seq on tissue? Please, go to: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080006">ATAC-seq package for tissue</a></p>',
'label1' => 'Method overview',
'info1' => '<p><strong>ATAC-seq</strong>, <strong>A</strong>ssay for <strong>T</strong>ransposase-<strong>A</strong>ccessible <strong>C</strong>hromatin, followed by next generation sequencing, is a key technology to easily identify the <strong>open regions of the chromatin.</strong> The protocol consists of <strong>3 steps</strong>: <strong>nuclei preparation</strong>, <strong>tagmentation</strong> and <strong>library amplification</strong>. First, the cells undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq.png" alt="ATAC-seq kit workflow" width="600px" caption="false" /></p>',
'label2' => 'Example of results',
'info2' => '<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig1.png" alt="library prepared with the Diagenode ATAC-seq kit " width="500px" caption="false" /></p>
<p><strong>Figure 1.</strong>Representative Bioanalyzer profile of an ATAC-seq library prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells.</p>
<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig2.png" alt="Diagenode ATAC-seq kit " caption="false" width="951" height="148" /></p>
<p><strong>Figure 2.</strong> Main ATAC-seq alignment and peak calling statistics of 3 replicates prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells. (Mapping efficiency: Percentage of non-mitochondrial reads that mapped to the reference genome. Uniquely mapped ratio: Proportion of mapped reads that map to only one location on the reference genome (hg19). Peaks: Number of peaks (open chromatin regions) identified by MACS2 for each sample. FRiP - Fraction of reads in peaks: Percentage of reads in peaks, with respect to the number of uniquely mapped reads. Sequencing was realized in paired-end mode 50 base pairs (PE50) on an Illumina NovaSeq6000.)</p>
<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig3a.png" alt="Assay for Transposase-Accessible Chromatin" width="500px" caption="false" /></p>
<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig3b.png" alt="Assay for Transposase-Accessible Chromatin" width="500px" caption="false" /></p>
<p><strong>Figure 3</strong> Sequencing profiles of ATAC-seq library (3 replicates) prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells.</p>
<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig4.png" alt=" open chromatin regions" caption="false" width="383" height="739" /></p>
<p><strong>Figure 4. </strong><br /> Heatmap around TSS of three ATAC-seq replicates prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells.</p>',
'label3' => 'Additional solutions for ATAC-seq kit',
'info3' => '<p><a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries">Primer indexes for tagmented libraries</a></p>
<p>Magnetic rack:<span> </span><a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag</a><a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit"><span> </span>0.2 ml – Cat. No. B04000001</a></p>
<p>Additional supplies (included in the kit and available separately):</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase</a><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30"><span> </span>(Tn5 transposase)<span> </span></a><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">loaded</a><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">, Cat. No. C01070012</a></li>
<li><a href="https://www.diagenode.com/en/p/tagmentation-buffer-2x">Tagmentation</a><a href="https://www.diagenode.com/en/p/tagmentation-buffer-2x"><span> </span>Buffer (2x), Cat. No. C01019043</a></li>
<li><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP</a><span> </span><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">DiaPure</a><span> </span><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">columns</a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">, Cat. No. C03040001</a></li>
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'meta_description' => 'Diagenode’s ATAC-seq kit provides a robust protocol for assessing genome-wide chromatin accessibility',
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'name' => 'iDeal CUT&Tag kit for Histones <br /> Compatible with histones and some non-histone proteins',
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<p><strong>CUT&Tag-sequencing</strong> (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins.</p>
<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
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<p>The Diagenode’s <strong>iDeal CUT&Tag kit for Histones</strong> provides an optimized protocol for a rapid chromatin profiling on <strong>histone marks</strong> and some <strong>non-histone proteins</strong>. The protocol is optimized for native cells (<strong>10,000-300,000</strong> cells per reaction) and can be completed within 1.5 days. The kit includes all reagents for cell processing, including CoA beads, pA-Tn5 and the DNA purification module. The antibodies (secondary antibodies, control antibodies) as well as primer indexes for multiplexing must be purchased separately.</p>
<p><strong>For a complete CUT&Tag protocol the following items must be purchased:</strong></p>
<ul>
<li><strong>iDeal CUT&Tag kit for Histones</strong> – including all reagents for CUT&Tag workflow (buffers, pA-Tn5, CoA beads, DNA purification)</li>
<li><strong>Antibody package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a></strong> or <strong><a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></strong> - including the secondary antibody, positive and negative control antibodies and primers</li>
<li><strong><a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries">Primer indexes for tagmented libraries</a></strong> – for multiplexing up to 72 samples</li>
</ul>
<h3>iDeal CUT&Tag Kit features:</h3>
<ul>
<li><strong>Rapid</strong> and <strong>easy </strong>chromatin profiling assay for <strong>histones </strong>and<strong> some non-histone proteins</strong></li>
<ul style="margin-bottom: 0;">
<li>No chromatin preparation</li>
<li>Easy sample handling due to ConA magnetic beads</li>
<li>Integrated library prep</li>
</ul>
<li><strong>Low cell number</strong>: 10,000-300,000 cells</li>
<li><strong>Accurate amplification</strong> due to intermediate quantification step</li>
<li><strong>High resolution</strong> and <strong>sensitivity</strong></li>
<li><strong>Lower sequencing depth</strong></li>
</ul>
<p>The quality of antibody used in CUT&Tag is one of the crucial factors for assay success. The antibodies with confirmed high specificity will target only the protein of interest, enabling real results. Check out our selection of <strong><a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">antibodies validated in CUT&Tag</a>.</strong></p>
<p>Looking for a standalone pA-Tn5? <a href="https://www.diagenode.com/en/products/view/3064">Read more</a>.</p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Method overview',
'info1' => '<p>The iDeal CUT&Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode’s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode’s Primer Indexes for tagmented libraries.</p>
<p></p>
<p><img alt=" pA-Tn5 Antibody package for CUT & Tag" src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></p>',
'label2' => 'Examples of results - histone marks',
'info2' => '<p>Successful CUT&Tag results showing a low background with high region-specific enrichment are presented below. Chromatin profiling has been performed on 50,000 K562 cells, using the Diagenode’s iDeal CUT&Tag kit for Histones (Cat. No. C01070020), the Antibody Package for CUT&Tag anti rabbit (Cat. No. C01070022), the 24 UDI for Tagmented Libraries (Cat.No. C01011034) and H3K4me3 (Cat. No. C15410003), H3K27me3 (Cat. No. C15410069) or H3H9me3 antibodies (Cat. No., C15410193) as indicated. The libraries were sequenced on Illumina’s NovaSeq6000 in 2x50 bp mode.</p>
<p><br /> <img alt="CUT&Tag-sequencing" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig1a.png" /> <img alt="CUT&Tag kit for Histones" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig1b.png" /> <img alt="Cleavage Under Targets and Tagmentation" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig1c.png" /></p>
<p><strong>Figure 1.</strong> Typical library profiles (Agilent Fragment traces) generated by the iDeal CUT&Tag protocol using 50,000 K562 cells and H3K27me3 (top), H3K4me4 (middle) primary antibodies and IgG control (bottom). Sharp peak at around 40 bp is an excess of <strong>free oligonucleotide used for pA-Tn5 loading</strong>.</p>
<p></p>
<p><br /><br /></p>
<div class="row">
<div class="small-4 columns">
<p><img alt="iDeal CUT&Tag kit for Histones" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig2.png" /></p>
</div>
<div class="small-8 columns">
<p><strong>Figure 2.</strong> Enrichments at TSS of the CUT&Tag libraries. The heatmap shows the enrichment around 3kb upstream and downstream of the TSS for H3K4me3. H3K4me3 as an active chromatin mark is associated with active promoters shows a narrow enrichment pattern.</p>
</div>
</div>
<p><br /><br /></p>
<p><br /> <img alt="iDeal CUT&Tag experiments of K562 cells " src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig3.png" /></p>
<p><strong>Figure 3.</strong> Sequencing profiles of the CUT&Tag libraries. Integrative genomics viewer (IGV) visualization of CUT&Tag experiments using 50,000 of K562 cells and H3K4me3 (blue), H3K27me3 (red) or H3K9me3 (green).</p>
<p><br /><br /></p>
<p><br /> <img alt="CUT&Tag experiments using 10,000 K562 cells" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig4.png" /></p>
<p><strong>Figure 4.</strong> Sequencing profiles of the CUT&Tag libraries. Integrative genomics viewer (IGV) visualization of CUT&Tag experiments using 50,000 (blue) or 10,000 (red)) of K562 cells and H3K27me3 antibody.</p>',
'label3' => 'Examples of results - transcription factors',
'info3' => '<p>Diagenode's<strong><span> iDeal CUT&Tag Kit for Histones</span></strong><span>, </span>developed for an efficient chromatin profiling on histone marks, can be used for profiling of <span>some transcription factors and co-factors using a mild fixation as described in the manual. </span></p>
<p><strong><span>Successful CUT&Tag results using CTCF and Suz12 antibodies and iDeal CUT&Tag Kit for Histones. </span></strong></p>
<p></p>
<p><span>Chromatin profiling of <strong>Suz12</strong> has been performed on 50,000 of fixed Mouse ES-E14TG2a cells (kindly provided by Luciano Di Croce, CRG, Spain) accordingly to the protocol of Diagenode’s iDeal CUT&Tag kit for Histones (Cat. No. C01070020). Antibody Package for CUT&Tag anti rabbit (Cat. No. C01070022) and the 24 UDI for Tagmented Libraries (Cat. No. C01011034) were used. The libraries were sized using Fragment Analyzer (Agilent) (triplicates, Figure 1, top). Relative enrichment has been confirmed by qPCR using know positive (T_Bra) and negative (Actb) loci (Figure 1, bottom). Libraries were sequenced on Illumina’s NovaSeq6000 in 2x50 bp mode.</span></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig1a.jpg" /><br /> <img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig1b.jpg" /></p>
<p><strong>Figure 1.</strong> Typical library profiles (top) and relative enrichment (bottom) generated by the iDeal CUT&Tag protocol using 50,000 ES-E14TG2a cells and Suz12 Antibody and IgG control (bottom). Sharp peak at around 40 bp is an excess of free oligonucleotide used for pA-Tn5 loading which does not interfere with the sequencing.</p>
<p></p>
<p>Pictures of representative loci were taken on Integrative Genome Viewer (IGV) for CTCF demonstrating the presence of high signal at promoter regions.</p>
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-Fig2a.jpg" /><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-Fig2b.jpg" /> <img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-Fig2c.jpg" /></p>
<p></p>
<p></p>
<p>Pictures of representative loci were taken on Integrative Genome Viewer (IGV) for SUZ12 and H3K27me3 triplicate samples, showing an overlap between the signal from both proteins, as part of the Polycomb Repressive Complex.</p>
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig2-loci1.jpg" width="100%" /><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig2-loci2.jpg" /><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig2-loci3.jpg" /></p>
<p><strong>Figure 2. IGV snapshots</strong></p>
<p></p>
<p></p>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig3.jpg" /></p>
</div>
<div class="small-8 columns">
<p><strong>Figure 3. Heatmap around the TSS</strong><br /> Heatmap of the CTCF signal around the transcription start sites (TSS) of each gene present in the murine mm10 reference genome.</p>
</div>
</div>
<p></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig4.jpg" /></p>
<p><strong>Figure 4. CTCF motif</strong><br /> Presence of specific transcription factor binding motifs in the regions identified as CTCF peaks. The top3 motifs are corresponding to the CTCF binding motif.</p>
<p></p>
<p></p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig5.jpg" /></p>
</div>
<div class="small-6 columns">
<p><strong>Figure 5. CTCF motif density</strong><br /> Location and density of the CTCF binding motif with respect to the center of the identified CTCF peaks.</p>
</div>
</div>
<p>
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'meta_title' => 'iDeal CUT&Tag Kit for Histones for chromatin profiling | Diagenode',
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'meta_description' => 'Rapid chromatin profiling assay, optimized protocol, all reagents provided. Easy sample handling due to ConA magnetic beads. Highly specific CUT&Tag grade antibodies available. ',
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'name' => 'ChIPmentation Kit for Histones',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/chipmentation-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p><b>ChIPmentation</b> is a method that combines <b>chromatin </b><b>immunoprecipiation</b> and <b>tagmentation</b><b>-based library preparation </b>using a fast and robust ChIP-seq protocol for studying <b>protein/DNA interactions</b>. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters. </p>
<p>The <b>ChIPmentation</b><b> Kit for Histones </b>includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The <b>primer indexes </b>for multiplexing are <b>not included</b> in the kit and have to be purchase separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">libraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
</ul>
<p><b>Benefits of the </b><b>ChIPmentation</b><b> system for histone </b><b>ChIP</b><b>-seq</b></p>
<ul>
<li>Easier and faster than classical ChIP-seq</li>
<li>Validated for various histone marks for a standard amount of cells</li>
<li>Generate high quality sequencing data</li>
</ul>
<p>For low input samples (10,000 cells) we recommend the <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation kit for Histones</a>.</p>
<p>For ChIP-seq on transcription factors we recommend the <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal</a> <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">ChIP-seq</a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns"> for transcription </a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">factors</a> + <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG kit for </a><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">ChIPmentation</a></p>',
'label1' => 'Validation',
'info1' => '<p>The Diagenode ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.</p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-1.png" /></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-2.png" /></div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-3.png" /></div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-4.png" /></div>
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<p><small><b>Figure 1: </b><b>ChIPmentation</b> <b>sequencing</b> <b>results</b> <b>obtained</b> <b>from</b> <b>decreasing</b> <b>starting</b> <b>amounts</b><b> of </b><b>cells</b><b>.<br /> </b><br /> Chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.</small></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-5.png" /></p>
<p><small><b>Figure 2: </b><b>ChIPmentation</b><b> sequencing results.</b></small></p>
<p>Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).</p>',
'label2' => 'Additional solutions compatible with ChIPmentation Kit for Histones ',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">iDeal</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells"> Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP</a><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for tagmenteted libraries:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">lilbraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
</ul>
<p>The kit ChIPmentation for Histones is validated on the <a href="https://www.diagenode.com/en/categories/ip-star">IP-Star Compact System </a>and the corresponding protocol is included in the manual.</p>',
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<p style="text-align: justify;">Diagenode’s epigenetic reagents include:</p>
<ul>
<li style="text-align: justify;"><strong>DNA methylation kits and antibodies</strong> - Validated NGS-compatible kits for MeDIP, MBD pull-down, whole genome bisulfite sequencing, and reduced representation bisulfite sequencing. Official provider for the original clone for 5-mC 33D3.</li>
<li style="text-align: justify;"><strong>ChIP and ChIP-seq kits for industry-leading specificity and sensitivity</strong> - MicroChIP/MicroPlex Kit for ChIP-seq with only 10,000 cells and the iDeal ChIP-seq Kits optimized for both transcription factors and histones. Our kits feature full reagents for ChIP-seq including control primers, control antibodies, magnetics beads, and purification reagents.</li>
<li style="text-align: justify;"><strong>Library preparation kits</strong> tailored for your specific requirements. The MicroPlex Library Preparation Kit simplifies library preparation requiring only 3 simple steps and allowing inputs of only 50 pg. </li>
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'description' => '<p>The <strong>Primer indexes for tagmented libraries</strong> are PCR primers targeting the Nextera sequencing adapters, previously incorporated in the libraries by <strong>tagmentation</strong>. Each primer index is baring a <strong>unique index</strong> in order to identify each library before pooling them for sequencing in the same lane. They are compatible with any Nextera-based libraries such as the one generated with ChIPmentation, ATAC-seq or CUT&Tag technologies.</p>
<h3>Features:</h3>
<ul>
<li>Compatibility: <strong>tagmentation-based library</strong> preparation protocols</li>
<li>Flexibility: <strong>Single</strong> and <strong>unique dual indexing</strong> available</li>
<li>Multiplexing capacity: up to <strong>72 samples</strong> (with UDI)</li>
<li>Identification and <strong>filtering of index hopping</strong> – using the UDI</li>
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<p>Get the manual of <a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf" target="_blank" title="Primer indexes for tagmented libraries - Manual">Primer indexes for tagmented libraries</a>.</p>',
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<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
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<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
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<p><b>ChIPmentation</b> is a method that combines <b>chromatin </b><b>immunoprecipiation</b> and <b>tagmentation</b><b>-based library preparation </b>using a fast and robust ChIP-seq protocol for studying <b>protein/DNA interactions</b>. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters. </p>
<p>The <b>ChIPmentation</b><b> Kit for Histones </b>includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The <b>primer indexes </b>for multiplexing are <b>not included</b> in the kit and have to be purchase separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">libraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
</ul>
<p><b>Benefits of the </b><b>ChIPmentation</b><b> system for histone </b><b>ChIP</b><b>-seq</b></p>
<ul>
<li>Easier and faster than classical ChIP-seq</li>
<li>Validated for various histone marks for a standard amount of cells</li>
<li>Generate high quality sequencing data</li>
</ul>
<p>For low input samples (10,000 cells) we recommend the <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation kit for Histones</a>.</p>
<p>For ChIP-seq on transcription factors we recommend the <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal</a> <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">ChIP-seq</a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns"> for transcription </a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">factors</a> + <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG kit for </a><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">ChIPmentation</a></p>',
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'info1' => '<p>The Diagenode ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.</p>
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<p><small><b>Figure 1: </b><b>ChIPmentation</b> <b>sequencing</b> <b>results</b> <b>obtained</b> <b>from</b> <b>decreasing</b> <b>starting</b> <b>amounts</b><b> of </b><b>cells</b><b>.<br /> </b><br /> Chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.</small></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-5.png" /></p>
<p><small><b>Figure 2: </b><b>ChIPmentation</b><b> sequencing results.</b></small></p>
<p>Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).</p>',
'label2' => 'Additional solutions compatible with ChIPmentation Kit for Histones ',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">iDeal</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells"> Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP</a><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for tagmenteted libraries:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">lilbraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
</ul>
<p>The kit ChIPmentation for Histones is validated on the <a href="https://www.diagenode.com/en/categories/ip-star">IP-Star Compact System </a>and the corresponding protocol is included in the manual.</p>',
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<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
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'name' => 'UMSBP2 is chromatin remodeler that functions in regulation of geneexpression and suppression of antigenic variation in trypanosomes.',
'authors' => 'Soni A. et al.',
'description' => '<p><span>Universal Minicircle Sequence binding proteins (UMSBPs) are CCHC-type zinc-finger proteins that bind the single-stranded G-rich UMS sequence, conserved at the replication origins of minicircles in the kinetoplast DNA, the mitochondrial genome of kinetoplastids. Trypanosoma brucei UMSBP2 has been recently shown to colocalize with telomeres and to play an essential role in chromosome end protection. Here we report that TbUMSBP2 decondenses in vitro DNA molecules, which were condensed by core histones H2B, H4 or linker histone H1. DNA decondensation is mediated via protein-protein interactions between TbUMSBP2 and these histones, independently of its previously described DNA binding activity. Silencing of the TbUMSBP2 gene resulted in a significant decrease in the disassembly of nucleosomes in T. brucei chromatin, a phenotype that could be reverted, by supplementing the knockdown cells with TbUMSBP2. Transcriptome analysis revealed that silencing of TbUMSBP2 affects the expression of multiple genes in T. brucei, with a most significant effect on the upregulation of the subtelomeric variant surface glycoproteins (VSG) genes, which mediate the antigenic variation in African trypanosomes. These observations suggest that UMSBP2 is a chromatin remodeling protein that functions in the regulation of gene expression and plays a role in the control of antigenic variation in T. brucei.</span></p>',
'date' => '2023-05-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37207337',
'doi' => '10.1093/nar/gkad402',
'modified' => '2023-06-15 08:54:17',
'created' => '2023-06-13 21:11:31',
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<p>3 sets of UDI for tagmented libraries are available:</p>
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<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
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<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
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<div class="extra-spaced">
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'info1' => '<p>ChIPmentation is based on tagmentation that allows library preparation to be integrated during the ChIP itself using transposase and sequencing-compatible adaptors. Our new improved µChIPmentation protocol combines 3 features for guaranteeing high quality sequencing data on small sample inputs 1) optimized chromatin shearing preparation protocol, 2) reduced number of steps, 3) reduced number of sample transfer from tube to tube - only 3 tubes per sample for the whole process, from cell fixation to purified libraries.</p>
<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"><i class="fa fa-caret-right"></i> Workflow of µChIPmentation for Histones - Read more</a>
<div id="workflow" class="content">
<div class="extra-spaced"><center><img src="https://www.diagenode.com/img/product/kits/workflow-micro-chipmentation.png" /></center></div>
</div>
</li>
</ul>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns"><strong>A.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1A.png" /></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-5 large-5 columns" columns=""><strong>B.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1B.png" /></div>
<div class="small-12 medium-7 large-7 columns">
<p style="text-align: left;"><strong>Figure 1. Comparison between ChIPmentation and µChIPmentation </strong><br />ChIPmentation: chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011000) and diluted chromatin from 500.000 cells was used for the immunoprecipitation. µChIPmentation: chromatin preparation and immunoprecipitation have been performed on 10.000 K562 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032). The Diagenode antibody targeting H3K27me3 (Cat. No. C15410195) was used.<br /> A. Distribution of the ChIPmentation and µChIPmentation readsets in a representative region of the genome (in duplicates). B. Comparison of the top 40% peaks from µChIPmentation (10.000 cells) with dataset generated with ChIPmentation (500.000 cells).</p>
</div>
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<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig2.png" /></div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 2. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
</div>
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</div>
<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig3.png" /></div>
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<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 3. Integrative genomics viewer (IGV) visualization of from ChIP-seq experiments using H3K27me3 antibody (Diagenode, Cat. No. <span>C15410195</span>) and 10.000 cells of K562 cells per immunoprecipitation.<br /></strong>Cells were FACS-sorted and ChIP has been performed accordingly to µChIPmentation protocol. Batch chromatin preparation followed by immunoprecipitation has been used per comparison as indicated.</p>
</div>
</div>
</div>',
'label2' => 'Additional solutions for µChIPmentation Kit for Histones',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit - High SDS</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for multiplexing:</p>
<ul class="no-bullet">
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries" target="_blank">24 SI for Tagmented libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries" target="_blank">8 SI for Tagmented libraries Cat. No. C01011033</a></li>
<li><em></em><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for Tagmented libraries, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
<li></li>
</ul>',
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<div align="center"><video width="400" height="250" autoplay="autoplay" muted="" loop="loop" controls="controls" src="https://www.diagenode.com/videos/chipmentation-dgo.mp4" frameborder="0" allowfullscreen="allowfullscreen"></video></div>
<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<p>Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fragments in one step. In our ChIPmentation technology we combine chromatin immunoprecipitation and tagmentation in one streamlined workflow where the tagmentation step occurs directly on chromatin. The <strong>TAG Kit for ChIPmentation</strong> has been developed for researchers who would like to perform ChIPmentation using their own validated ChIP protocol. Our TAG Kit for ChIPmentation includes reagents for <strong>tagmentation-based library preparation</strong> and is compatible with any ChIP protocol based on magnetic beads. The <strong>primer</strong> <strong>indexes for multiplexing</strong> must be <strong>purchased separately</strong> and are available as a reference:<br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank"><br /></a></p>',
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'info1' => '<h3>Comparing to classical ChIP-seq, ChIPmentation enables:</h3>
<ul>
<li>An easier protocol</li>
<li>Faster time to results</li>
<li>Flexibility – the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows you to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
</ul>
<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
<p><small>*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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'name' => 'ATAC-seq kit',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/atacseq-kit-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p><strong>ATAC-seq</strong>, Assay for<span> </span><strong>T</strong>ransposase-<strong>A</strong>ccessible<span> </span><strong>C</strong>hromatin, followed by next generation sequencing, is a key technology for genome-wide mapping of accessible chromatin. The technology is based on the use of the<span> </span><strong>transposase Tn5</strong><span> </span>which cuts exposed open chromatin and simultaneously ligates adapters for subsequent amplification and sequencing. ATAC-seq methods allow you to:</p>
<ul>
<li> Gain insight into gene regulation and understand open chromatin signatures</li>
<li> Determine nucleosome positions at single nucleotide resolution</li>
<li> Uncover transcription factor (TF) occupancy</li>
</ul>
</div>
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<p>Diagenode’s<span> </span><b>ATAC-</b><b>seq</b><b><span> </span>kit<span> </span></b>is based on a highly validated protocol optimized for<span> </span><b>50,000<span> </span></b><b>cells</b><b><span> </span>per<span> </span></b><b>reaction</b>. The kit includes the reagents for cell lysis and nuclei extraction, tagmentation and DNA purification as well as for library amplification. The <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries">primer indexes for multiplexing</a> are not included in the kit and must be purchased separately.</p>
<h4><span style="font-weight: 400;">ATAC-seq kit features:</span></h4>
<ul>
<li><b>Cell<span> </span></b><b>requirement</b><b>:<span> </span></b><b>50,000<span> </span></b><b>cells /<span> </span></b><b>rxn</b></li>
<li><b>Robust protocol<span> </span></b>with<span> </span><b>high reproducibility<span> </span></b>between replicates and repetitive experiments</li>
<li><strong>Easy</strong><span> </span>and<span> </span><b>efficient DNA capture<span> </span></b>after the tagmentation reaction using Diagenode`s MicroChIP DiaPure columns (included)</li>
<li>Additional qPCR step to determine the number of cycles needed for library amplification: </li>
<ul type="”square”">
<li><b>Avoids<span> </span></b><b>over-amplification</b></li>
<li>Allows adaptation/flexibility for<span> </span><b>more challenging samples<span> </span></b>to succeed with library prep.</li>
<li>Gives<span> </span><strong>early indication</strong><span> </span>if the experiment does not work (no qPCR amplification)</li>
</ul>
</ul>
<p>Looking for ATAC-seq on tissue? Please, go to: <a href="https://www.diagenode.com/en/p/ATAC-seq-package-tissue-C01080006">ATAC-seq package for tissue</a></p>',
'label1' => 'Method overview',
'info1' => '<p><strong>ATAC-seq</strong>, <strong>A</strong>ssay for <strong>T</strong>ransposase-<strong>A</strong>ccessible <strong>C</strong>hromatin, followed by next generation sequencing, is a key technology to easily identify the <strong>open regions of the chromatin.</strong> The protocol consists of <strong>3 steps</strong>: <strong>nuclei preparation</strong>, <strong>tagmentation</strong> and <strong>library amplification</strong>. First, the cells undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.</p>
<p><img src="https://www.diagenode.com/img/product/kits/workflow-atac-seq.png" alt="ATAC-seq kit workflow" width="600px" caption="false" /></p>',
'label2' => 'Example of results',
'info2' => '<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig1.png" alt="library prepared with the Diagenode ATAC-seq kit " width="500px" caption="false" /></p>
<p><strong>Figure 1.</strong>Representative Bioanalyzer profile of an ATAC-seq library prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells.</p>
<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig2.png" alt="Diagenode ATAC-seq kit " caption="false" width="951" height="148" /></p>
<p><strong>Figure 2.</strong> Main ATAC-seq alignment and peak calling statistics of 3 replicates prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells. (Mapping efficiency: Percentage of non-mitochondrial reads that mapped to the reference genome. Uniquely mapped ratio: Proportion of mapped reads that map to only one location on the reference genome (hg19). Peaks: Number of peaks (open chromatin regions) identified by MACS2 for each sample. FRiP - Fraction of reads in peaks: Percentage of reads in peaks, with respect to the number of uniquely mapped reads. Sequencing was realized in paired-end mode 50 base pairs (PE50) on an Illumina NovaSeq6000.)</p>
<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig3a.png" alt="Assay for Transposase-Accessible Chromatin" width="500px" caption="false" /></p>
<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig3b.png" alt="Assay for Transposase-Accessible Chromatin" width="500px" caption="false" /></p>
<p><strong>Figure 3</strong> Sequencing profiles of ATAC-seq library (3 replicates) prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells.</p>
<p><img src="https://www.diagenode.com/img/product/kits/atacseq-fig4.png" alt=" open chromatin regions" caption="false" width="383" height="739" /></p>
<p><strong>Figure 4. </strong><br /> Heatmap around TSS of three ATAC-seq replicates prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells.</p>',
'label3' => 'Additional solutions for ATAC-seq kit',
'info3' => '<p><a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries">Primer indexes for tagmented libraries</a></p>
<p>Magnetic rack:<span> </span><a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag</a><a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit"><span> </span>0.2 ml – Cat. No. B04000001</a></p>
<p>Additional supplies (included in the kit and available separately):</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase</a><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30"><span> </span>(Tn5 transposase)<span> </span></a><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">loaded</a><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">, Cat. No. C01070012</a></li>
<li><a href="https://www.diagenode.com/en/p/tagmentation-buffer-2x">Tagmentation</a><a href="https://www.diagenode.com/en/p/tagmentation-buffer-2x"><span> </span>Buffer (2x), Cat. No. C01019043</a></li>
<li><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP</a><span> </span><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">DiaPure</a><span> </span><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">columns</a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">, Cat. No. C03040001</a></li>
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'meta_description' => 'Diagenode’s ATAC-seq kit provides a robust protocol for assessing genome-wide chromatin accessibility',
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'name' => 'iDeal CUT&Tag kit for Histones <br /> Compatible with histones and some non-histone proteins',
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<p><strong>CUT&Tag-sequencing</strong> (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins.</p>
<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
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<p>The Diagenode’s <strong>iDeal CUT&Tag kit for Histones</strong> provides an optimized protocol for a rapid chromatin profiling on <strong>histone marks</strong> and some <strong>non-histone proteins</strong>. The protocol is optimized for native cells (<strong>10,000-300,000</strong> cells per reaction) and can be completed within 1.5 days. The kit includes all reagents for cell processing, including CoA beads, pA-Tn5 and the DNA purification module. The antibodies (secondary antibodies, control antibodies) as well as primer indexes for multiplexing must be purchased separately.</p>
<p><strong>For a complete CUT&Tag protocol the following items must be purchased:</strong></p>
<ul>
<li><strong>iDeal CUT&Tag kit for Histones</strong> – including all reagents for CUT&Tag workflow (buffers, pA-Tn5, CoA beads, DNA purification)</li>
<li><strong>Antibody package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a></strong> or <strong><a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></strong> - including the secondary antibody, positive and negative control antibodies and primers</li>
<li><strong><a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries">Primer indexes for tagmented libraries</a></strong> – for multiplexing up to 72 samples</li>
</ul>
<h3>iDeal CUT&Tag Kit features:</h3>
<ul>
<li><strong>Rapid</strong> and <strong>easy </strong>chromatin profiling assay for <strong>histones </strong>and<strong> some non-histone proteins</strong></li>
<ul style="margin-bottom: 0;">
<li>No chromatin preparation</li>
<li>Easy sample handling due to ConA magnetic beads</li>
<li>Integrated library prep</li>
</ul>
<li><strong>Low cell number</strong>: 10,000-300,000 cells</li>
<li><strong>Accurate amplification</strong> due to intermediate quantification step</li>
<li><strong>High resolution</strong> and <strong>sensitivity</strong></li>
<li><strong>Lower sequencing depth</strong></li>
</ul>
<p>The quality of antibody used in CUT&Tag is one of the crucial factors for assay success. The antibodies with confirmed high specificity will target only the protein of interest, enabling real results. Check out our selection of <strong><a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">antibodies validated in CUT&Tag</a>.</strong></p>
<p>Looking for a standalone pA-Tn5? <a href="https://www.diagenode.com/en/products/view/3064">Read more</a>.</p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Method overview',
'info1' => '<p>The iDeal CUT&Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode’s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode’s Primer Indexes for tagmented libraries.</p>
<p></p>
<p><img alt=" pA-Tn5 Antibody package for CUT & Tag" src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></p>',
'label2' => 'Examples of results - histone marks',
'info2' => '<p>Successful CUT&Tag results showing a low background with high region-specific enrichment are presented below. Chromatin profiling has been performed on 50,000 K562 cells, using the Diagenode’s iDeal CUT&Tag kit for Histones (Cat. No. C01070020), the Antibody Package for CUT&Tag anti rabbit (Cat. No. C01070022), the 24 UDI for Tagmented Libraries (Cat.No. C01011034) and H3K4me3 (Cat. No. C15410003), H3K27me3 (Cat. No. C15410069) or H3H9me3 antibodies (Cat. No., C15410193) as indicated. The libraries were sequenced on Illumina’s NovaSeq6000 in 2x50 bp mode.</p>
<p><br /> <img alt="CUT&Tag-sequencing" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig1a.png" /> <img alt="CUT&Tag kit for Histones" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig1b.png" /> <img alt="Cleavage Under Targets and Tagmentation" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig1c.png" /></p>
<p><strong>Figure 1.</strong> Typical library profiles (Agilent Fragment traces) generated by the iDeal CUT&Tag protocol using 50,000 K562 cells and H3K27me3 (top), H3K4me4 (middle) primary antibodies and IgG control (bottom). Sharp peak at around 40 bp is an excess of <strong>free oligonucleotide used for pA-Tn5 loading</strong>.</p>
<p></p>
<p><br /><br /></p>
<div class="row">
<div class="small-4 columns">
<p><img alt="iDeal CUT&Tag kit for Histones" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig2.png" /></p>
</div>
<div class="small-8 columns">
<p><strong>Figure 2.</strong> Enrichments at TSS of the CUT&Tag libraries. The heatmap shows the enrichment around 3kb upstream and downstream of the TSS for H3K4me3. H3K4me3 as an active chromatin mark is associated with active promoters shows a narrow enrichment pattern.</p>
</div>
</div>
<p><br /><br /></p>
<p><br /> <img alt="iDeal CUT&Tag experiments of K562 cells " src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig3.png" /></p>
<p><strong>Figure 3.</strong> Sequencing profiles of the CUT&Tag libraries. Integrative genomics viewer (IGV) visualization of CUT&Tag experiments using 50,000 of K562 cells and H3K4me3 (blue), H3K27me3 (red) or H3K9me3 (green).</p>
<p><br /><br /></p>
<p><br /> <img alt="CUT&Tag experiments using 10,000 K562 cells" src="https://www.diagenode.com/img/product/cutandtag/C01070020-cuttag-fig4.png" /></p>
<p><strong>Figure 4.</strong> Sequencing profiles of the CUT&Tag libraries. Integrative genomics viewer (IGV) visualization of CUT&Tag experiments using 50,000 (blue) or 10,000 (red)) of K562 cells and H3K27me3 antibody.</p>',
'label3' => 'Examples of results - transcription factors',
'info3' => '<p>Diagenode's<strong><span> iDeal CUT&Tag Kit for Histones</span></strong><span>, </span>developed for an efficient chromatin profiling on histone marks, can be used for profiling of <span>some transcription factors and co-factors using a mild fixation as described in the manual. </span></p>
<p><strong><span>Successful CUT&Tag results using CTCF and Suz12 antibodies and iDeal CUT&Tag Kit for Histones. </span></strong></p>
<p></p>
<p><span>Chromatin profiling of <strong>Suz12</strong> has been performed on 50,000 of fixed Mouse ES-E14TG2a cells (kindly provided by Luciano Di Croce, CRG, Spain) accordingly to the protocol of Diagenode’s iDeal CUT&Tag kit for Histones (Cat. No. C01070020). Antibody Package for CUT&Tag anti rabbit (Cat. No. C01070022) and the 24 UDI for Tagmented Libraries (Cat. No. C01011034) were used. The libraries were sized using Fragment Analyzer (Agilent) (triplicates, Figure 1, top). Relative enrichment has been confirmed by qPCR using know positive (T_Bra) and negative (Actb) loci (Figure 1, bottom). Libraries were sequenced on Illumina’s NovaSeq6000 in 2x50 bp mode.</span></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig1a.jpg" /><br /> <img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig1b.jpg" /></p>
<p><strong>Figure 1.</strong> Typical library profiles (top) and relative enrichment (bottom) generated by the iDeal CUT&Tag protocol using 50,000 ES-E14TG2a cells and Suz12 Antibody and IgG control (bottom). Sharp peak at around 40 bp is an excess of free oligonucleotide used for pA-Tn5 loading which does not interfere with the sequencing.</p>
<p></p>
<p>Pictures of representative loci were taken on Integrative Genome Viewer (IGV) for CTCF demonstrating the presence of high signal at promoter regions.</p>
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-Fig2a.jpg" /><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-Fig2b.jpg" /> <img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-Fig2c.jpg" /></p>
<p></p>
<p></p>
<p>Pictures of representative loci were taken on Integrative Genome Viewer (IGV) for SUZ12 and H3K27me3 triplicate samples, showing an overlap between the signal from both proteins, as part of the Polycomb Repressive Complex.</p>
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig2-loci1.jpg" width="100%" /><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig2-loci2.jpg" /><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig2-loci3.jpg" /></p>
<p><strong>Figure 2. IGV snapshots</strong></p>
<p></p>
<p></p>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig3.jpg" /></p>
</div>
<div class="small-8 columns">
<p><strong>Figure 3. Heatmap around the TSS</strong><br /> Heatmap of the CTCF signal around the transcription start sites (TSS) of each gene present in the murine mm10 reference genome.</p>
</div>
</div>
<p></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig4.jpg" /></p>
<p><strong>Figure 4. CTCF motif</strong><br /> Presence of specific transcription factor binding motifs in the regions identified as CTCF peaks. The top3 motifs are corresponding to the CTCF binding motif.</p>
<p></p>
<p></p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/cutandtag/C01070020-TF-fig5.jpg" /></p>
</div>
<div class="small-6 columns">
<p><strong>Figure 5. CTCF motif density</strong><br /> Location and density of the CTCF binding motif with respect to the center of the identified CTCF peaks.</p>
</div>
</div>
<p>
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'meta_title' => 'iDeal CUT&Tag Kit for Histones for chromatin profiling | Diagenode',
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'meta_description' => 'Rapid chromatin profiling assay, optimized protocol, all reagents provided. Easy sample handling due to ConA magnetic beads. Highly specific CUT&Tag grade antibodies available. ',
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'name' => 'ChIPmentation Kit for Histones',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/chipmentation-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p><b>ChIPmentation</b> is a method that combines <b>chromatin </b><b>immunoprecipiation</b> and <b>tagmentation</b><b>-based library preparation </b>using a fast and robust ChIP-seq protocol for studying <b>protein/DNA interactions</b>. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters. </p>
<p>The <b>ChIPmentation</b><b> Kit for Histones </b>includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The <b>primer indexes </b>for multiplexing are <b>not included</b> in the kit and have to be purchase separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">libraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
</ul>
<p><b>Benefits of the </b><b>ChIPmentation</b><b> system for histone </b><b>ChIP</b><b>-seq</b></p>
<ul>
<li>Easier and faster than classical ChIP-seq</li>
<li>Validated for various histone marks for a standard amount of cells</li>
<li>Generate high quality sequencing data</li>
</ul>
<p>For low input samples (10,000 cells) we recommend the <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation kit for Histones</a>.</p>
<p>For ChIP-seq on transcription factors we recommend the <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal</a> <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">ChIP-seq</a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns"> for transcription </a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">factors</a> + <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG kit for </a><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">ChIPmentation</a></p>',
'label1' => 'Validation',
'info1' => '<p>The Diagenode ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.</p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-1.png" /></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-2.png" /></div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-3.png" /></div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-4.png" /></div>
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<p><small><b>Figure 1: </b><b>ChIPmentation</b> <b>sequencing</b> <b>results</b> <b>obtained</b> <b>from</b> <b>decreasing</b> <b>starting</b> <b>amounts</b><b> of </b><b>cells</b><b>.<br /> </b><br /> Chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.</small></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-5.png" /></p>
<p><small><b>Figure 2: </b><b>ChIPmentation</b><b> sequencing results.</b></small></p>
<p>Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).</p>',
'label2' => 'Additional solutions compatible with ChIPmentation Kit for Histones ',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">iDeal</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells"> Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP</a><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for tagmenteted libraries:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">lilbraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
</ul>
<p>The kit ChIPmentation for Histones is validated on the <a href="https://www.diagenode.com/en/categories/ip-star">IP-Star Compact System </a>and the corresponding protocol is included in the manual.</p>',
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<p style="text-align: justify;">Diagenode’s epigenetic reagents include:</p>
<ul>
<li style="text-align: justify;"><strong>DNA methylation kits and antibodies</strong> - Validated NGS-compatible kits for MeDIP, MBD pull-down, whole genome bisulfite sequencing, and reduced representation bisulfite sequencing. Official provider for the original clone for 5-mC 33D3.</li>
<li style="text-align: justify;"><strong>ChIP and ChIP-seq kits for industry-leading specificity and sensitivity</strong> - MicroChIP/MicroPlex Kit for ChIP-seq with only 10,000 cells and the iDeal ChIP-seq Kits optimized for both transcription factors and histones. Our kits feature full reagents for ChIP-seq including control primers, control antibodies, magnetics beads, and purification reagents.</li>
<li style="text-align: justify;"><strong>Library preparation kits</strong> tailored for your specific requirements. The MicroPlex Library Preparation Kit simplifies library preparation requiring only 3 simple steps and allowing inputs of only 50 pg. </li>
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'description' => '<p>The <strong>Primer indexes for tagmented libraries</strong> are PCR primers targeting the Nextera sequencing adapters, previously incorporated in the libraries by <strong>tagmentation</strong>. Each primer index is baring a <strong>unique index</strong> in order to identify each library before pooling them for sequencing in the same lane. They are compatible with any Nextera-based libraries such as the one generated with ChIPmentation, ATAC-seq or CUT&Tag technologies.</p>
<h3>Features:</h3>
<ul>
<li>Compatibility: <strong>tagmentation-based library</strong> preparation protocols</li>
<li>Flexibility: <strong>Single</strong> and <strong>unique dual indexing</strong> available</li>
<li>Multiplexing capacity: up to <strong>72 samples</strong> (with UDI)</li>
<li>Identification and <strong>filtering of index hopping</strong> – using the UDI</li>
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<p>Get the manual of <a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf" target="_blank" title="Primer indexes for tagmented libraries - Manual">Primer indexes for tagmented libraries</a>.</p>',
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<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
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<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
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<p><b>ChIPmentation</b> is a method that combines <b>chromatin </b><b>immunoprecipiation</b> and <b>tagmentation</b><b>-based library preparation </b>using a fast and robust ChIP-seq protocol for studying <b>protein/DNA interactions</b>. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters. </p>
<p>The <b>ChIPmentation</b><b> Kit for Histones </b>includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The <b>primer indexes </b>for multiplexing are <b>not included</b> in the kit and have to be purchase separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">libraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
</ul>
<p><b>Benefits of the </b><b>ChIPmentation</b><b> system for histone </b><b>ChIP</b><b>-seq</b></p>
<ul>
<li>Easier and faster than classical ChIP-seq</li>
<li>Validated for various histone marks for a standard amount of cells</li>
<li>Generate high quality sequencing data</li>
</ul>
<p>For low input samples (10,000 cells) we recommend the <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation kit for Histones</a>.</p>
<p>For ChIP-seq on transcription factors we recommend the <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal</a> <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">ChIP-seq</a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns"> for transcription </a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">factors</a> + <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG kit for </a><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">ChIPmentation</a></p>',
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'info1' => '<p>The Diagenode ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.</p>
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<p><small><b>Figure 1: </b><b>ChIPmentation</b> <b>sequencing</b> <b>results</b> <b>obtained</b> <b>from</b> <b>decreasing</b> <b>starting</b> <b>amounts</b><b> of </b><b>cells</b><b>.<br /> </b><br /> Chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.</small></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-5.png" /></p>
<p><small><b>Figure 2: </b><b>ChIPmentation</b><b> sequencing results.</b></small></p>
<p>Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).</p>',
'label2' => 'Additional solutions compatible with ChIPmentation Kit for Histones ',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">iDeal</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells"> Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP</a><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for tagmenteted libraries:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">lilbraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
</ul>
<p>The kit ChIPmentation for Histones is validated on the <a href="https://www.diagenode.com/en/categories/ip-star">IP-Star Compact System </a>and the corresponding protocol is included in the manual.</p>',
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<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
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'id' => '4810',
'name' => 'UMSBP2 is chromatin remodeler that functions in regulation of geneexpression and suppression of antigenic variation in trypanosomes.',
'authors' => 'Soni A. et al.',
'description' => '<p><span>Universal Minicircle Sequence binding proteins (UMSBPs) are CCHC-type zinc-finger proteins that bind the single-stranded G-rich UMS sequence, conserved at the replication origins of minicircles in the kinetoplast DNA, the mitochondrial genome of kinetoplastids. Trypanosoma brucei UMSBP2 has been recently shown to colocalize with telomeres and to play an essential role in chromosome end protection. Here we report that TbUMSBP2 decondenses in vitro DNA molecules, which were condensed by core histones H2B, H4 or linker histone H1. DNA decondensation is mediated via protein-protein interactions between TbUMSBP2 and these histones, independently of its previously described DNA binding activity. Silencing of the TbUMSBP2 gene resulted in a significant decrease in the disassembly of nucleosomes in T. brucei chromatin, a phenotype that could be reverted, by supplementing the knockdown cells with TbUMSBP2. Transcriptome analysis revealed that silencing of TbUMSBP2 affects the expression of multiple genes in T. brucei, with a most significant effect on the upregulation of the subtelomeric variant surface glycoproteins (VSG) genes, which mediate the antigenic variation in African trypanosomes. These observations suggest that UMSBP2 is a chromatin remodeling protein that functions in the regulation of gene expression and plays a role in the control of antigenic variation in T. brucei.</span></p>',
'date' => '2023-05-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37207337',
'doi' => '10.1093/nar/gkad402',
'modified' => '2023-06-15 08:54:17',
'created' => '2023-06-13 21:11:31',
'ProductsPublication' => array(
'id' => '6972',
'product_id' => '3158',
'publication_id' => '4810'
)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/37207337" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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