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<p><b>ChIPmentation</b> is a method that combines <b>chromatin </b><b>immunoprecipiation</b> and <b>tagmentation</b><b>-based library preparation </b>using a fast and robust ChIP-seq protocol for studying <b>protein/DNA interactions</b>. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters. </p>
<p>The <b>ChIPmentation</b><b> Kit for Histones </b>includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The <b>primer indexes </b>for multiplexing are <b>not included</b> in the kit and have to be purchase separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">libraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
</ul>
<p><b>Benefits of the </b><b>ChIPmentation</b><b> system for histone </b><b>ChIP</b><b>-seq</b></p>
<ul>
<li>Easier and faster than classical ChIP-seq</li>
<li>Validated for various histone marks for a standard amount of cells</li>
<li>Generate high quality sequencing data</li>
</ul>
<p>For low input samples (10,000 cells) we recommend the <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation kit for Histones</a>.</p>
<p>For ChIP-seq on transcription factors we recommend the <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal</a> <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">ChIP-seq</a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns"> for transcription </a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">factors</a> + <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG kit for </a><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">ChIPmentation</a></p>',
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'info1' => '<p>The Diagenode ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.</p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-1.png" /></p>
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<p><small><b>Figure 1: </b><b>ChIPmentation</b> <b>sequencing</b> <b>results</b> <b>obtained</b> <b>from</b> <b>decreasing</b> <b>starting</b> <b>amounts</b><b> of </b><b>cells</b><b>.<br /> </b><br /> Chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.</small></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-5.png" /></p>
<p><small><b>Figure 2: </b><b>ChIPmentation</b><b> sequencing results.</b></small></p>
<p>Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).</p>',
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<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP</a><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for tagmenteted libraries:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">lilbraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
</ul>
<p>The kit ChIPmentation for Histones is validated on the <a href="https://www.diagenode.com/en/categories/ip-star">IP-Star Compact System </a>and the corresponding protocol is included in the manual.</p>',
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<p><b>ChIPmentation</b> is a method that combines <b>chromatin </b><b>immunoprecipiation</b> and <b>tagmentation</b><b>-based library preparation </b>using a fast and robust ChIP-seq protocol for studying <b>protein/DNA interactions</b>. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters. </p>
<p>The <b>ChIPmentation</b><b> Kit for Histones </b>includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The <b>primer indexes </b>for multiplexing are <b>not included</b> in the kit and have to be purchase separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">libraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
</ul>
<p><b>Benefits of the </b><b>ChIPmentation</b><b> system for histone </b><b>ChIP</b><b>-seq</b></p>
<ul>
<li>Easier and faster than classical ChIP-seq</li>
<li>Validated for various histone marks for a standard amount of cells</li>
<li>Generate high quality sequencing data</li>
</ul>
<p>For low input samples (10,000 cells) we recommend the <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation kit for Histones</a>.</p>
<p>For ChIP-seq on transcription factors we recommend the <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal</a> <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">ChIP-seq</a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns"> for transcription </a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">factors</a> + <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG kit for </a><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">ChIPmentation</a></p>',
'label1' => 'Validation',
'info1' => '<p>The Diagenode ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.</p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-1.png" /></p>
<div class="row">
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<p><small><b>Figure 1: </b><b>ChIPmentation</b> <b>sequencing</b> <b>results</b> <b>obtained</b> <b>from</b> <b>decreasing</b> <b>starting</b> <b>amounts</b><b> of </b><b>cells</b><b>.<br /> </b><br /> Chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.</small></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-5.png" /></p>
<p><small><b>Figure 2: </b><b>ChIPmentation</b><b> sequencing results.</b></small></p>
<p>Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).</p>',
'label2' => 'Additional solutions compatible with ChIPmentation Kit for Histones ',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">iDeal</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells"> Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP</a><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for tagmenteted libraries:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">lilbraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
</ul>
<p>The kit ChIPmentation for Histones is validated on the <a href="https://www.diagenode.com/en/categories/ip-star">IP-Star Compact System </a>and the corresponding protocol is included in the manual.</p>',
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'name' => 'Tagmentase (Tn5 transposase) - loaded',
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<p> </p>
<p>Diagenode <b>Tagmentase</b><b> – loaded </b>is a <strong>hyperactive Tn5 transposase</strong> preloaded with Illumina-compatible sequencing adapters. Its ability to cut DNA and insert the sequencing adapters in one step makes it the perfect companion for Next-Generation Sequencing experiments using powerful technologies such as ATAC-seq or ChIPmentation.</p>
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<p>Using Diagenode’s Tagmentase (Tn5 transposase) - loaded you may need also:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/tagmentation-buffer-1x-1ml">Tagmentation Buffer (1x)</a></li>
<li><a href="https://www.diagenode.com/en/p/tagmentation-buffer-2x">Tagmentation Buffer (2x)</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for tagmented libraries</a></li>
</ul>
<p>Looking for unloaded Tagmentase? Please go to <a href="https://www.diagenode.com/en/p/tagmentase-20-ul">Tagmentase</a><a href="https://www.diagenode.com/en/p/tagmentase-20-ul"> (Tn5 transposase) – </a><a href="https://www.diagenode.com/en/p/tagmentase-20-ul">unloaded</a></p>',
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'info1' => '<p><b>Tagmentase</b><b> (Tn5 transposase) – </b><b>loaded</b> can be used in any application using tagmentation-based library preparation, like for example ChIPmentation, ATAC-seq. Here below we are showing some examples of results.</p>
<center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig1.png" width="700" alt="Tn5 transposase Enxzymes" caption="false" /></center>
<p><small><strong>Figure 1. Typical library profile of ATAC-seq library generated with the Tagmentase</strong><br /> ATAC-seq has been performed on 50,000 nuclei from K562 cells using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043). The library profile has been generated on a Fragment Analyzer (Agilent).</small></p>
<div class="row">
<div class="small-8 columns">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig2.png" width="200" alt="Diagenode Tagmentation " caption="false" /></center></div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Enrichments at TSS of ATAC-seq libraries generated with the Tagmentase</strong><br />ATAC-seq has been performed on 50,000 nuclei from K562 cells, in triplicates, using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043). Heatmaps around the hg19 TSS were generated using deeptools plotHeatmap functionality.</small></p>
</div>
</div>
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<center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig3.png" alt="Transposase enzymes for ATAC-Seq" caption="false" width="956" height="259" /></center>
<p><small><strong>Figure 3. Sequencing profiles of ATAC-seq libraries generated with the Tagmentase </strong><br /> ATAC-seq has been performed on 50,000 nuclei from K562 cells, in triplicates, using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043).</small></p>
<center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig4.png" width="700" alt="purified Transposase enzymes " caption="false" /></center>
<p><small><strong>Figure 4. Typical library profile of ChIPmentation library generated with the Tagmentase</strong><br /> Chromatin preparation and immunoprecipitation have been performed on 1,000,000 K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011010) in combination with the Diagenode antibody targeting H3K4me3 (Cat. No. C15410003) and with the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012). The library profile has been generated on a Fragment Analyzer (Agilent).</small></p>
<center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig5.png" alt="pA-Tn5 Transposase Enzymes" caption="false" width="956" height="262" /></center>
<p><small><strong>Figure 5. Sequencing profiles of ChIPmentation libraries generated with the Tagmentase</strong><br /> Chromatin preparation and immunoprecipitation have been performed on 500.000 to 1,000,000 cells using the <a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones </a>(Cat. No. C01011010) in combination with the <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase) – loaded </a>(Cat. No. C01070012) on K562 cells. The Diagenode antibodies targeting <a href="https://www.diagenode.com/en/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">H3K4me3 </a>(Cat. No. C15410003), <a href="https://www.diagenode.com/en/p/h3k27ac-polyclonal-antibody-premium-50-mg-18-ml">H3K27ac </a>(Cat. No. C15410196), <a href="https://www.diagenode.com/en/p/h3k36me3-polyclonal-antibody-premium-50-mg">H3K36me3 </a>(Cat. No. C15410192), <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">H3K27me3</a> (Cat. No. C15410195) and rabbit <a href="https://www.diagenode.com/en/p/rabbit-igg-250-ug-250-ul">IgG </a>(Cat. No. C15410206) have been used.</small></p>
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<div>Protein Molecular weight: 53.3 kDa</div>
<p>Expressed: in Escherichia coli</p>
<p></p>
<p><strong>Product description:</strong> Diagenode Tagmentase – loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters. Its ability to cut DNA and insert the sequencing adapters in one step makes it the perfect companion for Next-Generation Sequencing experiments. The Tagmentase is pre-loaded with sequencing adapters compatible with Illumina Nextera platforms, as shown below. The oligos loaded on the Tagmentase are inserted into DNA upon a tagmentation reaction.</p>
<div>Mosaic end_reverse: 5’ [PHO]<span style="text-decoration: underline;">CTGTCTCTTATACACATCT</span> 3’</div>
<div>Mosaic end_Adapter A: 5’ TCGTCGGCAGCGTC<span style="text-decoration: underline;">AGATGTGTATAAGAGACAG</span> 3’</div>
<p>Mosaic end_Adapter B: 5’ GTCTCGTGGGCTCGG<span style="text-decoration: underline;">AGATGTGTATAAGAGACAG</span> 3’</p>
<p></p>
<div><span style="text-decoration: underline;">Underlined regions</span> correspond to the double-stranded part of the adapter, recognized by the Tagmentase. The final libraries can be amplified using Diagenode Primer indexes for tagmented libraries:</div>
<div>24 SI for tagmented libraries, Cat. No. C01011032</div>
<div>24 UDI fortagmented libraries - Set I, Cat. No. C0101134</div>
<div>24 UDI for tagmented libraries - Set II, Cat. No. C0101136</div>
<p>24 UDI for tagmented libraries - Set III, Cat. No. C0101137</p>
<p></p>
<p><strong>Storage conditions: </strong>Store at -20°C. Guaranteed stable for 6 months from date of receipt when stored properly.</p>
<p><strong>Storage buffer:</strong> Supplied in solution containing 50% v/v glycerol.</p>
<p><strong>Properties & Usage: </strong>Tagmentase is dependent on Mg++ for activity. Avoid chelators, such as EDTA/EGTA, in reaction buffers. The enzyme is active at pH 7.5-8 at 37-55°C. SDS, EDTA/EGTA or heating to 65°C will inactivate the enzyme.</p>
<p><strong>Applications:</strong> Tagmentase (Tn5 transposase) – loaded can be used in a variety of applications to construct library for second-generation sequencing. Please note that an additional optimization might be required for custom protocols including the enzyme dose- and time-response experiments.</p>
<div>For ATAC-seq and ChIPmentation, we recommend using Diagenode validated protocols:</div>
<div>ATAC-seq kit, Cat. No. C01080002</div>
<div>ATAC-seq package for tissue, Cat. No. <span>C01080006</span></div>
<div>ChIPmentation Kit for Histones, Cat. No. C01011009</div>
<div>µChIPmentation Kit for Histones, Cat. No. C01011011</div>
<div>TAG Kit for ChIPmentation, Cat. No. C01011030</div>
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<p>The Diagenode <strong>µChIPmentation Kit for Histones</strong> is optimized to perform ChIP-seq on as little as <strong>10.000 cells</strong> from cell fixation to purified libraries. To <strong>reduce DNA lost</strong> the number of sample transfer from tube to tube has been limited: during whole workflow - from cell fixation to library prurification - only 3 tubes per sample are used. Reduced number of steps, reduced number of sample transfer and <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation" target="_blank">ChIPmentation technology</a> itself enable for <strong>efficient and robust ChIP-seq</strong> on <strong>limited amount of sample</strong>. The kit µChIPmentation for Histones includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The primer indexes for multiplexing are not included in the kit and have to be purchase separately - <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">read more</a>.</p>
<div class="extra-spaced">
<h3>Benefits of the µChIPmentation system for histone ChIP-seq</h3>
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<li><strong>Easier</strong> and <strong>faster</strong> than classical ChIP-seq</li>
<li>Optimized for <strong>low input samples</strong>: as little as 10,000 cells</li>
<li>Protocol optimized for <strong>FACS-sorted cells</strong></li>
<li>Validated for various <strong>histone marks</strong></li>
<li><strong>High quality</strong> sequencing data</li>
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<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"><i class="fa fa-caret-right"></i> Workflow of µChIPmentation for Histones - Read more</a>
<div id="workflow" class="content">
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<div class="small-12 medium-12 large-12 columns"><strong>A.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1A.png" /></div>
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<div class="row extra-spaced">
<div class="small-12 medium-5 large-5 columns" columns=""><strong>B.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1B.png" /></div>
<div class="small-12 medium-7 large-7 columns">
<p style="text-align: left;"><strong>Figure 1. Comparison between ChIPmentation and µChIPmentation </strong><br />ChIPmentation: chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011000) and diluted chromatin from 500.000 cells was used for the immunoprecipitation. µChIPmentation: chromatin preparation and immunoprecipitation have been performed on 10.000 K562 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032). The Diagenode antibody targeting H3K27me3 (Cat. No. C15410195) was used.<br /> A. Distribution of the ChIPmentation and µChIPmentation readsets in a representative region of the genome (in duplicates). B. Comparison of the top 40% peaks from µChIPmentation (10.000 cells) with dataset generated with ChIPmentation (500.000 cells).</p>
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<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig2.png" /></div>
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<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 2. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
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<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig3.png" /></div>
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<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 3. Integrative genomics viewer (IGV) visualization of from ChIP-seq experiments using H3K27me3 antibody (Diagenode, Cat. No. <span>C15410195</span>) and 10.000 cells of K562 cells per immunoprecipitation.<br /></strong>Cells were FACS-sorted and ChIP has been performed accordingly to µChIPmentation protocol. Batch chromatin preparation followed by immunoprecipitation has been used per comparison as indicated.</p>
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<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
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<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for Tagmented libraries, Cat. No. C0101135</a></li>
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<li style="text-align: justify;"><strong>DNA methylation kits and antibodies</strong> - Validated NGS-compatible kits for MeDIP, MBD pull-down, whole genome bisulfite sequencing, and reduced representation bisulfite sequencing. Official provider for the original clone for 5-mC 33D3.</li>
<li style="text-align: justify;"><strong>ChIP and ChIP-seq kits for industry-leading specificity and sensitivity</strong> - MicroChIP/MicroPlex Kit for ChIP-seq with only 10,000 cells and the iDeal ChIP-seq Kits optimized for both transcription factors and histones. Our kits feature full reagents for ChIP-seq including control primers, control antibodies, magnetics beads, and purification reagents.</li>
<li style="text-align: justify;"><strong>Library preparation kits</strong> tailored for your specific requirements. The MicroPlex Library Preparation Kit simplifies library preparation requiring only 3 simple steps and allowing inputs of only 50 pg. </li>
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'id' => '5032',
'name' => 'Time-dependent chromatin maturation during 3D spheroid culture improves preclinical modeling of non-small cell lung cancer ',
'authors' => 'Anaïs Darracq et al.',
'description' => '<p><span>Non-small cell lung cancer (NSCLC) is the deadliest cancer worldwide. Therapeutic progress stagnate, highlighting the complexity to replicate NSCLC in preclinical models. Drug discovery studies rely mostly on cancer cells in two-dimension (2D), which poorly predict drug efficacy in patients. There is a growing interest in three-dimensional (3D) preclinical models, such as 3D spheroids, to better model tumor phenotype and improve therapeutic prediction. However, a comprehensive view of 3D culture methods impact on transcriptomes, epigenomes and pharmacological responses and their correlations to NSCLC tumors is still missing. Here, we demonstrate that NSCLC spheroids undergo time-dependent transcriptomic and epigenomic changes, which peak after 3 weeks of culture. While DNA methylome remained stable, chromatin methylation and acetylation marks gained features of advanced NSCLC in a time-dependent manner. Single-cell transcriptomic profiling of spheroids demonstrated that time of 3D culture improved the correlation to NSCLC tumors. Moreover, long-term culture of 3D spheroids increased drug screening predictability, by showing resistance to drugs that failed in NSCLC patients (such as HDAC inhibitors) while demonstrating novel pharmacological vulnerabilities and synergistic interactions (such as combination of PRMT and HDAC inhibitors). Strikingly, reverting 3D spheroids back to 2D culture rapidly reversed transcriptomic, epigenetic and pharmacological signatures acquired after 3 weeks of 3D culture, highlighting the critical impact of cell culture conditions on NSCLC phenotype. Collectively, our findings demonstrate that implementing a time-dependent maturation process into 3D spheroid culture induces chromatin and transcriptomic changes that enhance NSCLC preclinical modeling.</span></p>',
'date' => '2025-01-26',
'pmid' => 'https://www.biorxiv.org/content/10.1101/2025.01.23.634518v1',
'doi' => 'https://doi.org/10.1101/2025.01.23.634518',
'modified' => '2025-01-30 10:08:25',
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'name' => 'Epigenetics and suicide: investigating altered H3K14ac unveiled differential expression in ADORA2A, B4GALT2 and MMP14',
'authors' => 'Arčan IŠ et al.',
'description' => '<p><b>Background:</b><span><span> </span>Environmental factors make an important contribution to suicide. Histone tails are prone to different modifications, leading to changes of chromatin (de)condensation and consequently gene expression.<span> </span></span><b>Materials & methods:</b><span><span> </span>Level of H3K14ac was studied with chromatin immunoprecipitation followed by high-throughput DNA sequencing. Genes were further validated with RT-qPCR; using hippocampal tissue.<span> </span></span><b>Results:</b><span><span> </span>We showed lowered H3K14ac levels in individuals who died by suicide. The genes<span> </span></span><i>ADORA2A</i><span>,<span> </span></span><i>B4GALT2</i><span><span> </span>and<span> </span></span><i>MMP14</i><span><span> </span>showed differential expression in individuals who died by suicide. Identified genetic and protein interactions among genes show interactions with suicide-related genes.<span> </span></span><b>Conclusion:</b><span><span> </span>Further investigations of histone modifications in association with DNA methylation and miRNA are needed to expand our knowledge of the genes that could significantly contribute to suicide.</span></p>',
'date' => '2024-03-28',
'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/38545853/',
'doi' => '10.2217/epi-2023-0351',
'modified' => '2024-04-09 01:01:11',
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'id' => '4241',
'name' => 'Rhesus macaques self-curing from a schistosome infection can displaycomplete immunity to challenge',
'authors' => 'Amaral MS et al. ',
'description' => '<p>The rhesus macaque provides a unique model of acquired immunity against schistosomes, which afflict \>200 million people worldwide. By monitoring bloodstream levels of parasite-gut-derived antigen, we show that from week 10 onwards an established infection with Schistosoma mansoni is cleared in an exponential manner, eliciting resistance to reinfection. Secondary challenge at week 42 demonstrates that protection is strong in all animals and complete in some. Antibody profiles suggest that antigens mediating protection are the released products of developing schistosomula. In culture they are killed by addition of rhesus plasma, collected from week 8 post-infection onwards, and even more efficiently with post-challenge plasma. Furthermore, cultured schistosomula lose chromatin activating marks at the transcription start site of genes related to worm development and show decreased expression of genes related to lysosomes and lytic vacuoles involved with autophagy. Overall, our results indicate that enhanced antibody responses against the challenge migrating larvae mediate the naturally acquired protective immunity and will inform the route to an effective vaccine.</p>',
'date' => '2021-10-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34702841',
'doi' => '10.1038/s41467-021-26497-0',
'modified' => '2022-05-19 17:15:53',
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'name' => 'Niche stiffening compromises hair follicle stem cell potential duringageing by reducing bivalent promoter accessibility.',
'authors' => 'Koester Janis et al. ',
'description' => '<p>Tissue turnover requires activation and lineage commitment of tissue-resident stem cells (SCs). These processes are impacted by ageing, but the mechanisms remain unclear. Here, we addressed the mechanisms of ageing in murine hair follicle SCs (HFSCs) and observed a widespread reduction in chromatin accessibility in aged HFSCs, particularly at key self-renewal and differentiation genes, characterized by bivalent promoters occupied by active and repressive chromatin marks. Consistent with this, aged HFSCs showed reduced ability to activate bivalent genes for efficient self-renewal and differentiation. These defects were niche dependent as the transplantation of aged HFSCs into young recipients or synthetic niches restored SC functions. Mechanistically, the aged HFSC niche displayed widespread alterations in extracellular matrix composition and mechanics, resulting in mechanical stress and concomitant transcriptional repression to silence promoters. As a consequence, increasing basement membrane stiffness recapitulated age-related SC changes. These data identify niche mechanics as a central regulator of chromatin state, which, when altered, leads to age-dependent SC exhaustion.</p>',
'date' => '2021-07-01',
'pmid' => 'https://doi.org/10.1038%2Fs41556-021-00705-x',
'doi' => '10.1038/s41556-021-00705-x',
'modified' => '2022-06-22 12:34:49',
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'name' => 'A Tumor Suppressor Enhancer of PTEN in T-cell development and leukemia',
'authors' => 'L. Tottone at al.',
'description' => '<p>Long-range oncogenic enhancers play an important role in cancer. Yet, whether similar regulation of tumor suppressor genes is relevant remains unclear. Loss of expression of PTEN is associated with the pathogenesis of various cancers, including T-cell leukemia (T-ALL). Here, we identify a highly conserved distal enhancer (PE) that interacts with the <em>PTEN</em> promoter in multiple hematopoietic populations, including T-cells, and acts as a hub of relevant transcription factors in T-ALL. Consistently, loss of PE leads to reduced <em>PTEN</em> levels in T-ALL cells. Moreover, PE-null mice show reduced <em>Pten</em> levels in thymocytes and accelerated development of NOTCH1-induced T-ALL. Furthermore, secondary loss of PE in established leukemias leads to accelerated progression and a gene expression signature driven by <em>Pten</em> loss. Finally, we uncovered recurrent deletions encompassing PE in T-ALL, which are associated with decreased <em>PTEN</em> levels. Altogether, our results identify PE as the first long-range tumor suppressor enhancer directly implicated in cancer.</p>',
'date' => '2021-01-01',
'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/33458694/',
'doi' => '10.1158/2643-3230.BCD-20-0201 ',
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'name' => 'Epigenetic effects of low-level sodium arsenite exposure on human liver HepaRG cells',
'authors' => 'Tryndyak, V.P., Borowa-Mazgaj, B., Steward, C.R. et al. Epigenetic effects of low-level sodium arsenite exposure on human liver HepaRG cells.',
'description' => '<p>Chronic exposure to inorganic arsenic is associated with a variety of adverse health effects, including lung, bladder, kidney, and liver cancer. Several mechanisms have been proposed for arsenic-induced tumorigenesis; however, insufficient knowledge and many unanswered questions remain to explain the integrated molecular pathogenesis of arsenic carcinogenicity. In the present study, using non-tumorigenic human liver HepaRG cells, we investigated epigenetic alterations upon prolonged exposure to a noncytotoxic concentration of sodium arsenite (NaAsO<sub>2</sub>). We demonstrate that continuous exposure of HepaRG cells to 1 µM sodium arsenite (NaAsO<sub>2</sub>) for 14 days resulted in substantial cytosine DNA demethylation and hypermethylation across the genome, among which the claudin 14 (<i>CLDN14</i>) gene was hypermethylated and the most down-regulated gene. Another important finding was a profound loss of histone H3 lysine 36 (H3K36) trimethylation, which was accompanied by increased damage to genomic DNA and an elevated de novo mutation frequency. These results demonstrate that continuous exposure of HepaRG cells to a noncytotoxic concentration of NaAsO<sub>2</sub> results in substantial epigenetic abnormalities accompanied by several carcinogenesis-related events, including induction of epithelial-to-mesenchymal transition, damage to DNA, inhibition of DNA repair genes, and induction of de novo mutations. Importantly, this study highlights the intimate mechanistic link and interplay between two fundamental cancer-associated events, epigenetic and genetic alterations, in arsenic-associated carcinogenesis.</p>',
'date' => '2020-08-25',
'pmid' => 'https://link.springer.com/article/10.1007/s00204-020-02872-6',
'doi' => 'https://doi.org/10.1007/s00204-020-02872-6',
'modified' => '2020-09-11 15:08:45',
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'name' => 'Distinct and temporary-restricted epigenetic mechanisms regulate human αβ and γδ T cell development ',
'authors' => 'Roels J, Kuchmiy A, De Decker M, et al. ',
'description' => '<p>The development of TCRαβ and TCRγδ T cells comprises a step-wise process in which regulatory events control differentiation and lineage outcome. To clarify these mechanisms, we employed RNA-sequencing, ATAC-sequencing and ChIPmentation on well-defined thymocyte subsets that represent the continuum of human T cell development. The chromatin accessibility dynamics show clear stage specificity and reveal that human T cell-lineage commitment is marked by GATA3- and BCL11B-dependent closing of PU.1 sites. A temporary increase in H3K27me3 without open chromatin modifications is unique for β-selection, whereas emerging γδ T cells, which originate from common precursors of β-selected cells, show large chromatin accessibility changes due to strong T cell receptor (TCR) signaling. Furthermore, we unravel distinct chromatin landscapes between CD4<sup>+</sup> and CD8<sup>+</sup> αβ-lineage cells that support their effector functions and reveal gene-specific mechanisms that define mature T cells. This resource provides a framework for studying gene regulatory mechanisms that drive normal and malignant human T cell development.</p>',
'date' => '2020-07-27',
'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/32719521/',
'doi' => ' 10.1038/s41590-020-0747-9 ',
'modified' => '2021-01-29 14:12:02',
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'name' => 'Measuring Histone Modifications in the Human Parasite Schistosoma mansoni ',
'authors' => 'de Carvalho Augusto R, Roquis D, Al Picard M, Chaparro C, Cosseau C, Grunau C.',
'description' => '<p>DNA-binding proteins play critical roles in many major processes such as development and sexual biology of Schistosoma mansoni and are important for the pathogenesis of schistosomiasis. Chromatin immunoprecipitation (ChIP) experiments followed by sequencing (ChIP-seq) are useful to characterize the association of genomic regions with posttranslational chemical modifications of histone proteins. Challenges in the standard ChIP protocol have motivated recent enhancements in this approach, such as reducing the number of cells required and increasing the resolution. In this chapter, we describe the latest advances made by our group in the ChIP methods to improve the standard ChIP protocol to reduce the number of input cells required and to increase the resolution and robustness of ChIP in S. mansoni.</p>',
'date' => '2020-05-26',
'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/32451999/',
'doi' => '10.1007/978-1-0716-0635-3_9 ',
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<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Tagmentase (Tn5 transposase) - loaded</strong> to my shopping cart.</p>
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'name' => 'µChIPmentation Kit for Histones',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/microchipmentation-for-histones.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The Diagenode <strong>µChIPmentation Kit for Histones</strong> is optimized to perform ChIP-seq on as little as <strong>10.000 cells</strong> from cell fixation to purified libraries. To <strong>reduce DNA lost</strong> the number of sample transfer from tube to tube has been limited: during whole workflow - from cell fixation to library prurification - only 3 tubes per sample are used. Reduced number of steps, reduced number of sample transfer and <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation" target="_blank">ChIPmentation technology</a> itself enable for <strong>efficient and robust ChIP-seq</strong> on <strong>limited amount of sample</strong>. The kit µChIPmentation for Histones includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The primer indexes for multiplexing are not included in the kit and have to be purchase separately - <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">read more</a>.</p>
<div class="extra-spaced">
<h3>Benefits of the µChIPmentation system for histone ChIP-seq</h3>
<ul>
<li><strong>Easier</strong> and <strong>faster</strong> than classical ChIP-seq</li>
<li>Optimized for <strong>low input samples</strong>: as little as 10,000 cells</li>
<li>Protocol optimized for <strong>FACS-sorted cells</strong></li>
<li>Validated for various <strong>histone marks</strong></li>
<li><strong>High quality</strong> sequencing data</li>
</ul>
</div>',
'label1' => 'Characteristics',
'info1' => '<p>ChIPmentation is based on tagmentation that allows library preparation to be integrated during the ChIP itself using transposase and sequencing-compatible adaptors. Our new improved µChIPmentation protocol combines 3 features for guaranteeing high quality sequencing data on small sample inputs 1) optimized chromatin shearing preparation protocol, 2) reduced number of steps, 3) reduced number of sample transfer from tube to tube - only 3 tubes per sample for the whole process, from cell fixation to purified libraries.</p>
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<div class="small-12 medium-7 large-7 columns">
<p style="text-align: left;"><strong>Figure 1. Comparison between ChIPmentation and µChIPmentation </strong><br />ChIPmentation: chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011000) and diluted chromatin from 500.000 cells was used for the immunoprecipitation. µChIPmentation: chromatin preparation and immunoprecipitation have been performed on 10.000 K562 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032). The Diagenode antibody targeting H3K27me3 (Cat. No. C15410195) was used.<br /> A. Distribution of the ChIPmentation and µChIPmentation readsets in a representative region of the genome (in duplicates). B. Comparison of the top 40% peaks from µChIPmentation (10.000 cells) with dataset generated with ChIPmentation (500.000 cells).</p>
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<p><strong>Figure 2. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
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<p><strong>Figure 3. Integrative genomics viewer (IGV) visualization of from ChIP-seq experiments using H3K27me3 antibody (Diagenode, Cat. No. <span>C15410195</span>) and 10.000 cells of K562 cells per immunoprecipitation.<br /></strong>Cells were FACS-sorted and ChIP has been performed accordingly to µChIPmentation protocol. Batch chromatin preparation followed by immunoprecipitation has been used per comparison as indicated.</p>
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'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit - High SDS</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for multiplexing:</p>
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<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries" target="_blank">8 SI for Tagmented libraries Cat. No. C01011033</a></li>
<li><em></em><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for Tagmented libraries, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
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'description' => '<p>Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Two versions of protocol have been validated: a manual procedure and an automated protocol on the<span style="font-weight: 400;"> IP-Star Compact Automated System.</span></p>',
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'description' => '<p>DNA-binding proteins play critical roles in many major processes such as development and sexual biology of Schistosoma mansoni and are important for the pathogenesis of schistosomiasis. Chromatin immunoprecipitation (ChIP) experiments followed by sequencing (ChIP-seq) are useful to characterize the association of genomic regions with posttranslational chemical modifications of histone proteins. Challenges in the standard ChIP protocol have motivated recent enhancements in this approach, such as reducing the number of cells required and increasing the resolution. In this chapter, we describe the latest advances made by our group in the ChIP methods to improve the standard ChIP protocol to reduce the number of input cells required and to increase the resolution and robustness of ChIP in S. mansoni.</p>',
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<p><b>ChIPmentation</b> is a method that combines <b>chromatin </b><b>immunoprecipiation</b> and <b>tagmentation</b><b>-based library preparation </b>using a fast and robust ChIP-seq protocol for studying <b>protein/DNA interactions</b>. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters. </p>
<p>The <b>ChIPmentation</b><b> Kit for Histones </b>includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The <b>primer indexes </b>for multiplexing are <b>not included</b> in the kit and have to be purchase separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">libraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
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<p><b>Benefits of the </b><b>ChIPmentation</b><b> system for histone </b><b>ChIP</b><b>-seq</b></p>
<ul>
<li>Easier and faster than classical ChIP-seq</li>
<li>Validated for various histone marks for a standard amount of cells</li>
<li>Generate high quality sequencing data</li>
</ul>
<p>For low input samples (10,000 cells) we recommend the <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation kit for Histones</a>.</p>
<p>For ChIP-seq on transcription factors we recommend the <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal</a> <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">ChIP-seq</a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns"> for transcription </a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">factors</a> + <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG kit for </a><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">ChIPmentation</a></p>',
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'info1' => '<p>The Diagenode ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.</p>
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<p><small><b>Figure 1: </b><b>ChIPmentation</b> <b>sequencing</b> <b>results</b> <b>obtained</b> <b>from</b> <b>decreasing</b> <b>starting</b> <b>amounts</b><b> of </b><b>cells</b><b>.<br /> </b><br /> Chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.</small></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-5.png" /></p>
<p><small><b>Figure 2: </b><b>ChIPmentation</b><b> sequencing results.</b></small></p>
<p>Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).</p>',
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'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">iDeal</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells"> Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP</a><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for tagmenteted libraries:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">lilbraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
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<p>The kit ChIPmentation for Histones is validated on the <a href="https://www.diagenode.com/en/categories/ip-star">IP-Star Compact System </a>and the corresponding protocol is included in the manual.</p>',
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<p><b>ChIPmentation</b> is a method that combines <b>chromatin </b><b>immunoprecipiation</b> and <b>tagmentation</b><b>-based library preparation </b>using a fast and robust ChIP-seq protocol for studying <b>protein/DNA interactions</b>. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters. </p>
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<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">libraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
</ul>
<p><b>Benefits of the </b><b>ChIPmentation</b><b> system for histone </b><b>ChIP</b><b>-seq</b></p>
<ul>
<li>Easier and faster than classical ChIP-seq</li>
<li>Validated for various histone marks for a standard amount of cells</li>
<li>Generate high quality sequencing data</li>
</ul>
<p>For low input samples (10,000 cells) we recommend the <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation kit for Histones</a>.</p>
<p>For ChIP-seq on transcription factors we recommend the <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal</a> <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">ChIP-seq</a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns"> for transcription </a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">factors</a> + <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG kit for </a><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">ChIPmentation</a></p>',
'label1' => 'Validation',
'info1' => '<p>The Diagenode ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.</p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-1.png" /></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-2.png" /></div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-3.png" /></div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-4.png" /></div>
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<p><small><b>Figure 1: </b><b>ChIPmentation</b> <b>sequencing</b> <b>results</b> <b>obtained</b> <b>from</b> <b>decreasing</b> <b>starting</b> <b>amounts</b><b> of </b><b>cells</b><b>.<br /> </b><br /> Chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.</small></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-5.png" /></p>
<p><small><b>Figure 2: </b><b>ChIPmentation</b><b> sequencing results.</b></small></p>
<p>Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).</p>',
'label2' => 'Additional solutions compatible with ChIPmentation Kit for Histones ',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">iDeal</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells"> Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP</a><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for tagmenteted libraries:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">lilbraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
</ul>
<p>The kit ChIPmentation for Histones is validated on the <a href="https://www.diagenode.com/en/categories/ip-star">IP-Star Compact System </a>and the corresponding protocol is included in the manual.</p>',
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<p> </p>
<p>Diagenode <b>Tagmentase</b><b> – loaded </b>is a <strong>hyperactive Tn5 transposase</strong> preloaded with Illumina-compatible sequencing adapters. Its ability to cut DNA and insert the sequencing adapters in one step makes it the perfect companion for Next-Generation Sequencing experiments using powerful technologies such as ATAC-seq or ChIPmentation.</p>
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<p>Using Diagenode’s Tagmentase (Tn5 transposase) - loaded you may need also:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/tagmentation-buffer-1x-1ml">Tagmentation Buffer (1x)</a></li>
<li><a href="https://www.diagenode.com/en/p/tagmentation-buffer-2x">Tagmentation Buffer (2x)</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for tagmented libraries</a></li>
</ul>
<p>Looking for unloaded Tagmentase? Please go to <a href="https://www.diagenode.com/en/p/tagmentase-20-ul">Tagmentase</a><a href="https://www.diagenode.com/en/p/tagmentase-20-ul"> (Tn5 transposase) – </a><a href="https://www.diagenode.com/en/p/tagmentase-20-ul">unloaded</a></p>',
'label1' => 'Examples of results',
'info1' => '<p><b>Tagmentase</b><b> (Tn5 transposase) – </b><b>loaded</b> can be used in any application using tagmentation-based library preparation, like for example ChIPmentation, ATAC-seq. Here below we are showing some examples of results.</p>
<center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig1.png" width="700" alt="Tn5 transposase Enxzymes" caption="false" /></center>
<p><small><strong>Figure 1. Typical library profile of ATAC-seq library generated with the Tagmentase</strong><br /> ATAC-seq has been performed on 50,000 nuclei from K562 cells using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043). The library profile has been generated on a Fragment Analyzer (Agilent).</small></p>
<div class="row">
<div class="small-8 columns">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig2.png" width="200" alt="Diagenode Tagmentation " caption="false" /></center></div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Enrichments at TSS of ATAC-seq libraries generated with the Tagmentase</strong><br />ATAC-seq has been performed on 50,000 nuclei from K562 cells, in triplicates, using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043). Heatmaps around the hg19 TSS were generated using deeptools plotHeatmap functionality.</small></p>
</div>
</div>
</div>
<center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig3.png" alt="Transposase enzymes for ATAC-Seq" caption="false" width="956" height="259" /></center>
<p><small><strong>Figure 3. Sequencing profiles of ATAC-seq libraries generated with the Tagmentase </strong><br /> ATAC-seq has been performed on 50,000 nuclei from K562 cells, in triplicates, using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043).</small></p>
<center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig4.png" width="700" alt="purified Transposase enzymes " caption="false" /></center>
<p><small><strong>Figure 4. Typical library profile of ChIPmentation library generated with the Tagmentase</strong><br /> Chromatin preparation and immunoprecipitation have been performed on 1,000,000 K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011010) in combination with the Diagenode antibody targeting H3K4me3 (Cat. No. C15410003) and with the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012). The library profile has been generated on a Fragment Analyzer (Agilent).</small></p>
<center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig5.png" alt="pA-Tn5 Transposase Enzymes" caption="false" width="956" height="262" /></center>
<p><small><strong>Figure 5. Sequencing profiles of ChIPmentation libraries generated with the Tagmentase</strong><br /> Chromatin preparation and immunoprecipitation have been performed on 500.000 to 1,000,000 cells using the <a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones </a>(Cat. No. C01011010) in combination with the <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase) – loaded </a>(Cat. No. C01070012) on K562 cells. The Diagenode antibodies targeting <a href="https://www.diagenode.com/en/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">H3K4me3 </a>(Cat. No. C15410003), <a href="https://www.diagenode.com/en/p/h3k27ac-polyclonal-antibody-premium-50-mg-18-ml">H3K27ac </a>(Cat. No. C15410196), <a href="https://www.diagenode.com/en/p/h3k36me3-polyclonal-antibody-premium-50-mg">H3K36me3 </a>(Cat. No. C15410192), <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">H3K27me3</a> (Cat. No. C15410195) and rabbit <a href="https://www.diagenode.com/en/p/rabbit-igg-250-ug-250-ul">IgG </a>(Cat. No. C15410206) have been used.</small></p>
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'info2' => '<p><strong>Tagmentase (Tn5 transposase) - loaded </strong></p>
<div>Protein Molecular weight: 53.3 kDa</div>
<p>Expressed: in Escherichia coli</p>
<p></p>
<p><strong>Product description:</strong> Diagenode Tagmentase – loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters. Its ability to cut DNA and insert the sequencing adapters in one step makes it the perfect companion for Next-Generation Sequencing experiments. The Tagmentase is pre-loaded with sequencing adapters compatible with Illumina Nextera platforms, as shown below. The oligos loaded on the Tagmentase are inserted into DNA upon a tagmentation reaction.</p>
<div>Mosaic end_reverse: 5’ [PHO]<span style="text-decoration: underline;">CTGTCTCTTATACACATCT</span> 3’</div>
<div>Mosaic end_Adapter A: 5’ TCGTCGGCAGCGTC<span style="text-decoration: underline;">AGATGTGTATAAGAGACAG</span> 3’</div>
<p>Mosaic end_Adapter B: 5’ GTCTCGTGGGCTCGG<span style="text-decoration: underline;">AGATGTGTATAAGAGACAG</span> 3’</p>
<p></p>
<div><span style="text-decoration: underline;">Underlined regions</span> correspond to the double-stranded part of the adapter, recognized by the Tagmentase. The final libraries can be amplified using Diagenode Primer indexes for tagmented libraries:</div>
<div>24 SI for tagmented libraries, Cat. No. C01011032</div>
<div>24 UDI fortagmented libraries - Set I, Cat. No. C0101134</div>
<div>24 UDI for tagmented libraries - Set II, Cat. No. C0101136</div>
<p>24 UDI for tagmented libraries - Set III, Cat. No. C0101137</p>
<p></p>
<p><strong>Storage conditions: </strong>Store at -20°C. Guaranteed stable for 6 months from date of receipt when stored properly.</p>
<p><strong>Storage buffer:</strong> Supplied in solution containing 50% v/v glycerol.</p>
<p><strong>Properties & Usage: </strong>Tagmentase is dependent on Mg++ for activity. Avoid chelators, such as EDTA/EGTA, in reaction buffers. The enzyme is active at pH 7.5-8 at 37-55°C. SDS, EDTA/EGTA or heating to 65°C will inactivate the enzyme.</p>
<p><strong>Applications:</strong> Tagmentase (Tn5 transposase) – loaded can be used in a variety of applications to construct library for second-generation sequencing. Please note that an additional optimization might be required for custom protocols including the enzyme dose- and time-response experiments.</p>
<div>For ATAC-seq and ChIPmentation, we recommend using Diagenode validated protocols:</div>
<div>ATAC-seq kit, Cat. No. C01080002</div>
<div>ATAC-seq package for tissue, Cat. No. <span>C01080006</span></div>
<div>ChIPmentation Kit for Histones, Cat. No. C01011009</div>
<div>µChIPmentation Kit for Histones, Cat. No. C01011011</div>
<div>TAG Kit for ChIPmentation, Cat. No. C01011030</div>
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'name' => 'µChIPmentation Kit for Histones',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/microchipmentation-for-histones.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The Diagenode <strong>µChIPmentation Kit for Histones</strong> is optimized to perform ChIP-seq on as little as <strong>10.000 cells</strong> from cell fixation to purified libraries. To <strong>reduce DNA lost</strong> the number of sample transfer from tube to tube has been limited: during whole workflow - from cell fixation to library prurification - only 3 tubes per sample are used. Reduced number of steps, reduced number of sample transfer and <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation" target="_blank">ChIPmentation technology</a> itself enable for <strong>efficient and robust ChIP-seq</strong> on <strong>limited amount of sample</strong>. The kit µChIPmentation for Histones includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The primer indexes for multiplexing are not included in the kit and have to be purchase separately - <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">read more</a>.</p>
<div class="extra-spaced">
<h3>Benefits of the µChIPmentation system for histone ChIP-seq</h3>
<ul>
<li><strong>Easier</strong> and <strong>faster</strong> than classical ChIP-seq</li>
<li>Optimized for <strong>low input samples</strong>: as little as 10,000 cells</li>
<li>Protocol optimized for <strong>FACS-sorted cells</strong></li>
<li>Validated for various <strong>histone marks</strong></li>
<li><strong>High quality</strong> sequencing data</li>
</ul>
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'info1' => '<p>ChIPmentation is based on tagmentation that allows library preparation to be integrated during the ChIP itself using transposase and sequencing-compatible adaptors. Our new improved µChIPmentation protocol combines 3 features for guaranteeing high quality sequencing data on small sample inputs 1) optimized chromatin shearing preparation protocol, 2) reduced number of steps, 3) reduced number of sample transfer from tube to tube - only 3 tubes per sample for the whole process, from cell fixation to purified libraries.</p>
<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"><i class="fa fa-caret-right"></i> Workflow of µChIPmentation for Histones - Read more</a>
<div id="workflow" class="content">
<div class="extra-spaced"><center><img src="https://www.diagenode.com/img/product/kits/workflow-micro-chipmentation.png" /></center></div>
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<div class="small-12 medium-5 large-5 columns" columns=""><strong>B.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1B.png" /></div>
<div class="small-12 medium-7 large-7 columns">
<p style="text-align: left;"><strong>Figure 1. Comparison between ChIPmentation and µChIPmentation </strong><br />ChIPmentation: chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011000) and diluted chromatin from 500.000 cells was used for the immunoprecipitation. µChIPmentation: chromatin preparation and immunoprecipitation have been performed on 10.000 K562 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032). The Diagenode antibody targeting H3K27me3 (Cat. No. C15410195) was used.<br /> A. Distribution of the ChIPmentation and µChIPmentation readsets in a representative region of the genome (in duplicates). B. Comparison of the top 40% peaks from µChIPmentation (10.000 cells) with dataset generated with ChIPmentation (500.000 cells).</p>
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<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 2. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
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<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 3. Integrative genomics viewer (IGV) visualization of from ChIP-seq experiments using H3K27me3 antibody (Diagenode, Cat. No. <span>C15410195</span>) and 10.000 cells of K562 cells per immunoprecipitation.<br /></strong>Cells were FACS-sorted and ChIP has been performed accordingly to µChIPmentation protocol. Batch chromatin preparation followed by immunoprecipitation has been used per comparison as indicated.</p>
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<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for multiplexing:</p>
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<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries" target="_blank">24 SI for Tagmented libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries" target="_blank">8 SI for Tagmented libraries Cat. No. C01011033</a></li>
<li><em></em><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for Tagmented libraries, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
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<p style="text-align: justify;">Diagenode’s epigenetic reagents include:</p>
<ul>
<li style="text-align: justify;"><strong>DNA methylation kits and antibodies</strong> - Validated NGS-compatible kits for MeDIP, MBD pull-down, whole genome bisulfite sequencing, and reduced representation bisulfite sequencing. Official provider for the original clone for 5-mC 33D3.</li>
<li style="text-align: justify;"><strong>ChIP and ChIP-seq kits for industry-leading specificity and sensitivity</strong> - MicroChIP/MicroPlex Kit for ChIP-seq with only 10,000 cells and the iDeal ChIP-seq Kits optimized for both transcription factors and histones. Our kits feature full reagents for ChIP-seq including control primers, control antibodies, magnetics beads, and purification reagents.</li>
<li style="text-align: justify;"><strong>Library preparation kits</strong> tailored for your specific requirements. The MicroPlex Library Preparation Kit simplifies library preparation requiring only 3 simple steps and allowing inputs of only 50 pg. </li>
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'description' => '<p>Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Two versions of protocol have been validated: a manual procedure and an automated protocol on the<span style="font-weight: 400;"> IP-Star Compact Automated System.</span></p>',
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'name' => 'Time-dependent chromatin maturation during 3D spheroid culture improves preclinical modeling of non-small cell lung cancer ',
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'description' => '<p><span>Non-small cell lung cancer (NSCLC) is the deadliest cancer worldwide. Therapeutic progress stagnate, highlighting the complexity to replicate NSCLC in preclinical models. Drug discovery studies rely mostly on cancer cells in two-dimension (2D), which poorly predict drug efficacy in patients. There is a growing interest in three-dimensional (3D) preclinical models, such as 3D spheroids, to better model tumor phenotype and improve therapeutic prediction. However, a comprehensive view of 3D culture methods impact on transcriptomes, epigenomes and pharmacological responses and their correlations to NSCLC tumors is still missing. Here, we demonstrate that NSCLC spheroids undergo time-dependent transcriptomic and epigenomic changes, which peak after 3 weeks of culture. While DNA methylome remained stable, chromatin methylation and acetylation marks gained features of advanced NSCLC in a time-dependent manner. Single-cell transcriptomic profiling of spheroids demonstrated that time of 3D culture improved the correlation to NSCLC tumors. Moreover, long-term culture of 3D spheroids increased drug screening predictability, by showing resistance to drugs that failed in NSCLC patients (such as HDAC inhibitors) while demonstrating novel pharmacological vulnerabilities and synergistic interactions (such as combination of PRMT and HDAC inhibitors). Strikingly, reverting 3D spheroids back to 2D culture rapidly reversed transcriptomic, epigenetic and pharmacological signatures acquired after 3 weeks of 3D culture, highlighting the critical impact of cell culture conditions on NSCLC phenotype. Collectively, our findings demonstrate that implementing a time-dependent maturation process into 3D spheroid culture induces chromatin and transcriptomic changes that enhance NSCLC preclinical modeling.</span></p>',
'date' => '2025-01-26',
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'name' => 'Epigenetics and suicide: investigating altered H3K14ac unveiled differential expression in ADORA2A, B4GALT2 and MMP14',
'authors' => 'Arčan IŠ et al.',
'description' => '<p><b>Background:</b><span><span> </span>Environmental factors make an important contribution to suicide. Histone tails are prone to different modifications, leading to changes of chromatin (de)condensation and consequently gene expression.<span> </span></span><b>Materials & methods:</b><span><span> </span>Level of H3K14ac was studied with chromatin immunoprecipitation followed by high-throughput DNA sequencing. Genes were further validated with RT-qPCR; using hippocampal tissue.<span> </span></span><b>Results:</b><span><span> </span>We showed lowered H3K14ac levels in individuals who died by suicide. The genes<span> </span></span><i>ADORA2A</i><span>,<span> </span></span><i>B4GALT2</i><span><span> </span>and<span> </span></span><i>MMP14</i><span><span> </span>showed differential expression in individuals who died by suicide. Identified genetic and protein interactions among genes show interactions with suicide-related genes.<span> </span></span><b>Conclusion:</b><span><span> </span>Further investigations of histone modifications in association with DNA methylation and miRNA are needed to expand our knowledge of the genes that could significantly contribute to suicide.</span></p>',
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'name' => 'Rhesus macaques self-curing from a schistosome infection can displaycomplete immunity to challenge',
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'description' => '<p>The rhesus macaque provides a unique model of acquired immunity against schistosomes, which afflict \>200 million people worldwide. By monitoring bloodstream levels of parasite-gut-derived antigen, we show that from week 10 onwards an established infection with Schistosoma mansoni is cleared in an exponential manner, eliciting resistance to reinfection. Secondary challenge at week 42 demonstrates that protection is strong in all animals and complete in some. Antibody profiles suggest that antigens mediating protection are the released products of developing schistosomula. In culture they are killed by addition of rhesus plasma, collected from week 8 post-infection onwards, and even more efficiently with post-challenge plasma. Furthermore, cultured schistosomula lose chromatin activating marks at the transcription start site of genes related to worm development and show decreased expression of genes related to lysosomes and lytic vacuoles involved with autophagy. Overall, our results indicate that enhanced antibody responses against the challenge migrating larvae mediate the naturally acquired protective immunity and will inform the route to an effective vaccine.</p>',
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'name' => 'Niche stiffening compromises hair follicle stem cell potential duringageing by reducing bivalent promoter accessibility.',
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'description' => '<p>Tissue turnover requires activation and lineage commitment of tissue-resident stem cells (SCs). These processes are impacted by ageing, but the mechanisms remain unclear. Here, we addressed the mechanisms of ageing in murine hair follicle SCs (HFSCs) and observed a widespread reduction in chromatin accessibility in aged HFSCs, particularly at key self-renewal and differentiation genes, characterized by bivalent promoters occupied by active and repressive chromatin marks. Consistent with this, aged HFSCs showed reduced ability to activate bivalent genes for efficient self-renewal and differentiation. These defects were niche dependent as the transplantation of aged HFSCs into young recipients or synthetic niches restored SC functions. Mechanistically, the aged HFSC niche displayed widespread alterations in extracellular matrix composition and mechanics, resulting in mechanical stress and concomitant transcriptional repression to silence promoters. As a consequence, increasing basement membrane stiffness recapitulated age-related SC changes. These data identify niche mechanics as a central regulator of chromatin state, which, when altered, leads to age-dependent SC exhaustion.</p>',
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'name' => 'A Tumor Suppressor Enhancer of PTEN in T-cell development and leukemia',
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'date' => '2021-01-01',
'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/33458694/',
'doi' => '10.1158/2643-3230.BCD-20-0201 ',
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'name' => 'Epigenetic effects of low-level sodium arsenite exposure on human liver HepaRG cells',
'authors' => 'Tryndyak, V.P., Borowa-Mazgaj, B., Steward, C.R. et al. Epigenetic effects of low-level sodium arsenite exposure on human liver HepaRG cells.',
'description' => '<p>Chronic exposure to inorganic arsenic is associated with a variety of adverse health effects, including lung, bladder, kidney, and liver cancer. Several mechanisms have been proposed for arsenic-induced tumorigenesis; however, insufficient knowledge and many unanswered questions remain to explain the integrated molecular pathogenesis of arsenic carcinogenicity. In the present study, using non-tumorigenic human liver HepaRG cells, we investigated epigenetic alterations upon prolonged exposure to a noncytotoxic concentration of sodium arsenite (NaAsO<sub>2</sub>). We demonstrate that continuous exposure of HepaRG cells to 1 µM sodium arsenite (NaAsO<sub>2</sub>) for 14 days resulted in substantial cytosine DNA demethylation and hypermethylation across the genome, among which the claudin 14 (<i>CLDN14</i>) gene was hypermethylated and the most down-regulated gene. Another important finding was a profound loss of histone H3 lysine 36 (H3K36) trimethylation, which was accompanied by increased damage to genomic DNA and an elevated de novo mutation frequency. These results demonstrate that continuous exposure of HepaRG cells to a noncytotoxic concentration of NaAsO<sub>2</sub> results in substantial epigenetic abnormalities accompanied by several carcinogenesis-related events, including induction of epithelial-to-mesenchymal transition, damage to DNA, inhibition of DNA repair genes, and induction of de novo mutations. Importantly, this study highlights the intimate mechanistic link and interplay between two fundamental cancer-associated events, epigenetic and genetic alterations, in arsenic-associated carcinogenesis.</p>',
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'pmid' => 'https://link.springer.com/article/10.1007/s00204-020-02872-6',
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'description' => '<p>The development of TCRαβ and TCRγδ T cells comprises a step-wise process in which regulatory events control differentiation and lineage outcome. To clarify these mechanisms, we employed RNA-sequencing, ATAC-sequencing and ChIPmentation on well-defined thymocyte subsets that represent the continuum of human T cell development. The chromatin accessibility dynamics show clear stage specificity and reveal that human T cell-lineage commitment is marked by GATA3- and BCL11B-dependent closing of PU.1 sites. A temporary increase in H3K27me3 without open chromatin modifications is unique for β-selection, whereas emerging γδ T cells, which originate from common precursors of β-selected cells, show large chromatin accessibility changes due to strong T cell receptor (TCR) signaling. Furthermore, we unravel distinct chromatin landscapes between CD4<sup>+</sup> and CD8<sup>+</sup> αβ-lineage cells that support their effector functions and reveal gene-specific mechanisms that define mature T cells. This resource provides a framework for studying gene regulatory mechanisms that drive normal and malignant human T cell development.</p>',
'date' => '2020-07-27',
'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/32719521/',
'doi' => ' 10.1038/s41590-020-0747-9 ',
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'description' => '<p>DNA-binding proteins play critical roles in many major processes such as development and sexual biology of Schistosoma mansoni and are important for the pathogenesis of schistosomiasis. Chromatin immunoprecipitation (ChIP) experiments followed by sequencing (ChIP-seq) are useful to characterize the association of genomic regions with posttranslational chemical modifications of histone proteins. Challenges in the standard ChIP protocol have motivated recent enhancements in this approach, such as reducing the number of cells required and increasing the resolution. In this chapter, we describe the latest advances made by our group in the ChIP methods to improve the standard ChIP protocol to reduce the number of input cells required and to increase the resolution and robustness of ChIP in S. mansoni.</p>',
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<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> µChIPmentation Kit for Histones</strong> to my shopping cart.</p>
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<p>The Diagenode <strong>µChIPmentation Kit for Histones</strong> is optimized to perform ChIP-seq on as little as <strong>10.000 cells</strong> from cell fixation to purified libraries. To <strong>reduce DNA lost</strong> the number of sample transfer from tube to tube has been limited: during whole workflow - from cell fixation to library prurification - only 3 tubes per sample are used. Reduced number of steps, reduced number of sample transfer and <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation" target="_blank">ChIPmentation technology</a> itself enable for <strong>efficient and robust ChIP-seq</strong> on <strong>limited amount of sample</strong>. The kit µChIPmentation for Histones includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The primer indexes for multiplexing are not included in the kit and have to be purchase separately - <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">read more</a>.</p>
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<h3>Benefits of the µChIPmentation system for histone ChIP-seq</h3>
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<li><strong>Easier</strong> and <strong>faster</strong> than classical ChIP-seq</li>
<li>Optimized for <strong>low input samples</strong>: as little as 10,000 cells</li>
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<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"><i class="fa fa-caret-right"></i> Workflow of µChIPmentation for Histones - Read more</a>
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<p style="text-align: left;"><strong>Figure 1. Comparison between ChIPmentation and µChIPmentation </strong><br />ChIPmentation: chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011000) and diluted chromatin from 500.000 cells was used for the immunoprecipitation. µChIPmentation: chromatin preparation and immunoprecipitation have been performed on 10.000 K562 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032). The Diagenode antibody targeting H3K27me3 (Cat. No. C15410195) was used.<br /> A. Distribution of the ChIPmentation and µChIPmentation readsets in a representative region of the genome (in duplicates). B. Comparison of the top 40% peaks from µChIPmentation (10.000 cells) with dataset generated with ChIPmentation (500.000 cells).</p>
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<p><strong>Figure 2. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
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<p><strong>Figure 3. Integrative genomics viewer (IGV) visualization of from ChIP-seq experiments using H3K27me3 antibody (Diagenode, Cat. No. <span>C15410195</span>) and 10.000 cells of K562 cells per immunoprecipitation.<br /></strong>Cells were FACS-sorted and ChIP has been performed accordingly to µChIPmentation protocol. Batch chromatin preparation followed by immunoprecipitation has been used per comparison as indicated.</p>
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<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
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'description' => '<p>Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Two versions of protocol have been validated: a manual procedure and an automated protocol on the<span style="font-weight: 400;"> IP-Star Compact Automated System.</span></p>',
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'authors' => 'de Carvalho Augusto R, Roquis D, Al Picard M, Chaparro C, Cosseau C, Grunau C.',
'description' => '<p>DNA-binding proteins play critical roles in many major processes such as development and sexual biology of Schistosoma mansoni and are important for the pathogenesis of schistosomiasis. Chromatin immunoprecipitation (ChIP) experiments followed by sequencing (ChIP-seq) are useful to characterize the association of genomic regions with posttranslational chemical modifications of histone proteins. Challenges in the standard ChIP protocol have motivated recent enhancements in this approach, such as reducing the number of cells required and increasing the resolution. In this chapter, we describe the latest advances made by our group in the ChIP methods to improve the standard ChIP protocol to reduce the number of input cells required and to increase the resolution and robustness of ChIP in S. mansoni.</p>',
'date' => '2020-05-26',
'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/32451999/',
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'description' => '<p><a href="https://www.diagenode.com/files/products/kits/chipmentation-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p><b>ChIPmentation</b> is a method that combines <b>chromatin </b><b>immunoprecipiation</b> and <b>tagmentation</b><b>-based library preparation </b>using a fast and robust ChIP-seq protocol for studying <b>protein/DNA interactions</b>. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters. </p>
<p>The <b>ChIPmentation</b><b> Kit for Histones </b>includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The <b>primer indexes </b>for multiplexing are <b>not included</b> in the kit and have to be purchase separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">libraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
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<p><b>Benefits of the </b><b>ChIPmentation</b><b> system for histone </b><b>ChIP</b><b>-seq</b></p>
<ul>
<li>Easier and faster than classical ChIP-seq</li>
<li>Validated for various histone marks for a standard amount of cells</li>
<li>Generate high quality sequencing data</li>
</ul>
<p>For low input samples (10,000 cells) we recommend the <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation kit for Histones</a>.</p>
<p>For ChIP-seq on transcription factors we recommend the <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal</a> <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">ChIP-seq</a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns"> for transcription </a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">factors</a> + <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG kit for </a><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">ChIPmentation</a></p>',
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<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-1.png" /></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-2.png" /></div>
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<p><small><b>Figure 1: </b><b>ChIPmentation</b> <b>sequencing</b> <b>results</b> <b>obtained</b> <b>from</b> <b>decreasing</b> <b>starting</b> <b>amounts</b><b> of </b><b>cells</b><b>.<br /> </b><br /> Chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.</small></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-5.png" /></p>
<p><small><b>Figure 2: </b><b>ChIPmentation</b><b> sequencing results.</b></small></p>
<p>Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).</p>',
'label2' => 'Additional solutions compatible with ChIPmentation Kit for Histones ',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">iDeal</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells"> Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP</a><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for tagmenteted libraries:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">lilbraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
</ul>
<p>The kit ChIPmentation for Histones is validated on the <a href="https://www.diagenode.com/en/categories/ip-star">IP-Star Compact System </a>and the corresponding protocol is included in the manual.</p>',
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'description' => '<p><a href="https://www.diagenode.com/files/products/kits/chipmentation-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p><b>ChIPmentation</b> is a method that combines <b>chromatin </b><b>immunoprecipiation</b> and <b>tagmentation</b><b>-based library preparation </b>using a fast and robust ChIP-seq protocol for studying <b>protein/DNA interactions</b>. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters. </p>
<p>The <b>ChIPmentation</b><b> Kit for Histones </b>includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The <b>primer indexes </b>for multiplexing are <b>not included</b> in the kit and have to be purchase separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">libraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
</ul>
<p><b>Benefits of the </b><b>ChIPmentation</b><b> system for histone </b><b>ChIP</b><b>-seq</b></p>
<ul>
<li>Easier and faster than classical ChIP-seq</li>
<li>Validated for various histone marks for a standard amount of cells</li>
<li>Generate high quality sequencing data</li>
</ul>
<p>For low input samples (10,000 cells) we recommend the <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation kit for Histones</a>.</p>
<p>For ChIP-seq on transcription factors we recommend the <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal</a> <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">ChIP-seq</a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns"> for transcription </a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">factors</a> + <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG kit for </a><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">ChIPmentation</a></p>',
'label1' => 'Validation',
'info1' => '<p>The Diagenode ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.</p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-1.png" /></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-2.png" /></div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-3.png" /></div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-4.png" /></div>
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<p><small><b>Figure 1: </b><b>ChIPmentation</b> <b>sequencing</b> <b>results</b> <b>obtained</b> <b>from</b> <b>decreasing</b> <b>starting</b> <b>amounts</b><b> of </b><b>cells</b><b>.<br /> </b><br /> Chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.</small></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-5.png" /></p>
<p><small><b>Figure 2: </b><b>ChIPmentation</b><b> sequencing results.</b></small></p>
<p>Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).</p>',
'label2' => 'Additional solutions compatible with ChIPmentation Kit for Histones ',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">iDeal</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells"> Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP</a><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for tagmenteted libraries:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">lilbraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
</ul>
<p>The kit ChIPmentation for Histones is validated on the <a href="https://www.diagenode.com/en/categories/ip-star">IP-Star Compact System </a>and the corresponding protocol is included in the manual.</p>',
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<p> </p>
<p>Diagenode <b>Tagmentase</b><b> – loaded </b>is a <strong>hyperactive Tn5 transposase</strong> preloaded with Illumina-compatible sequencing adapters. Its ability to cut DNA and insert the sequencing adapters in one step makes it the perfect companion for Next-Generation Sequencing experiments using powerful technologies such as ATAC-seq or ChIPmentation.</p>
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<p>Using Diagenode’s Tagmentase (Tn5 transposase) - loaded you may need also:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/tagmentation-buffer-1x-1ml">Tagmentation Buffer (1x)</a></li>
<li><a href="https://www.diagenode.com/en/p/tagmentation-buffer-2x">Tagmentation Buffer (2x)</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for tagmented libraries</a></li>
</ul>
<p>Looking for unloaded Tagmentase? Please go to <a href="https://www.diagenode.com/en/p/tagmentase-20-ul">Tagmentase</a><a href="https://www.diagenode.com/en/p/tagmentase-20-ul"> (Tn5 transposase) – </a><a href="https://www.diagenode.com/en/p/tagmentase-20-ul">unloaded</a></p>',
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'info1' => '<p><b>Tagmentase</b><b> (Tn5 transposase) – </b><b>loaded</b> can be used in any application using tagmentation-based library preparation, like for example ChIPmentation, ATAC-seq. Here below we are showing some examples of results.</p>
<center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig1.png" width="700" alt="Tn5 transposase Enxzymes" caption="false" /></center>
<p><small><strong>Figure 1. Typical library profile of ATAC-seq library generated with the Tagmentase</strong><br /> ATAC-seq has been performed on 50,000 nuclei from K562 cells using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043). The library profile has been generated on a Fragment Analyzer (Agilent).</small></p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig2.png" width="200" alt="Diagenode Tagmentation " caption="false" /></center></div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Enrichments at TSS of ATAC-seq libraries generated with the Tagmentase</strong><br />ATAC-seq has been performed on 50,000 nuclei from K562 cells, in triplicates, using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043). Heatmaps around the hg19 TSS were generated using deeptools plotHeatmap functionality.</small></p>
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<center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig3.png" alt="Transposase enzymes for ATAC-Seq" caption="false" width="956" height="259" /></center>
<p><small><strong>Figure 3. Sequencing profiles of ATAC-seq libraries generated with the Tagmentase </strong><br /> ATAC-seq has been performed on 50,000 nuclei from K562 cells, in triplicates, using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043).</small></p>
<center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig4.png" width="700" alt="purified Transposase enzymes " caption="false" /></center>
<p><small><strong>Figure 4. Typical library profile of ChIPmentation library generated with the Tagmentase</strong><br /> Chromatin preparation and immunoprecipitation have been performed on 1,000,000 K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011010) in combination with the Diagenode antibody targeting H3K4me3 (Cat. No. C15410003) and with the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012). The library profile has been generated on a Fragment Analyzer (Agilent).</small></p>
<center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig5.png" alt="pA-Tn5 Transposase Enzymes" caption="false" width="956" height="262" /></center>
<p><small><strong>Figure 5. Sequencing profiles of ChIPmentation libraries generated with the Tagmentase</strong><br /> Chromatin preparation and immunoprecipitation have been performed on 500.000 to 1,000,000 cells using the <a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones </a>(Cat. No. C01011010) in combination with the <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase) – loaded </a>(Cat. No. C01070012) on K562 cells. The Diagenode antibodies targeting <a href="https://www.diagenode.com/en/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">H3K4me3 </a>(Cat. No. C15410003), <a href="https://www.diagenode.com/en/p/h3k27ac-polyclonal-antibody-premium-50-mg-18-ml">H3K27ac </a>(Cat. No. C15410196), <a href="https://www.diagenode.com/en/p/h3k36me3-polyclonal-antibody-premium-50-mg">H3K36me3 </a>(Cat. No. C15410192), <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">H3K27me3</a> (Cat. No. C15410195) and rabbit <a href="https://www.diagenode.com/en/p/rabbit-igg-250-ug-250-ul">IgG </a>(Cat. No. C15410206) have been used.</small></p>
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'info2' => '<p><strong>Tagmentase (Tn5 transposase) - loaded </strong></p>
<div>Protein Molecular weight: 53.3 kDa</div>
<p>Expressed: in Escherichia coli</p>
<p></p>
<p><strong>Product description:</strong> Diagenode Tagmentase – loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters. Its ability to cut DNA and insert the sequencing adapters in one step makes it the perfect companion for Next-Generation Sequencing experiments. The Tagmentase is pre-loaded with sequencing adapters compatible with Illumina Nextera platforms, as shown below. The oligos loaded on the Tagmentase are inserted into DNA upon a tagmentation reaction.</p>
<div>Mosaic end_reverse: 5’ [PHO]<span style="text-decoration: underline;">CTGTCTCTTATACACATCT</span> 3’</div>
<div>Mosaic end_Adapter A: 5’ TCGTCGGCAGCGTC<span style="text-decoration: underline;">AGATGTGTATAAGAGACAG</span> 3’</div>
<p>Mosaic end_Adapter B: 5’ GTCTCGTGGGCTCGG<span style="text-decoration: underline;">AGATGTGTATAAGAGACAG</span> 3’</p>
<p></p>
<div><span style="text-decoration: underline;">Underlined regions</span> correspond to the double-stranded part of the adapter, recognized by the Tagmentase. The final libraries can be amplified using Diagenode Primer indexes for tagmented libraries:</div>
<div>24 SI for tagmented libraries, Cat. No. C01011032</div>
<div>24 UDI fortagmented libraries - Set I, Cat. No. C0101134</div>
<div>24 UDI for tagmented libraries - Set II, Cat. No. C0101136</div>
<p>24 UDI for tagmented libraries - Set III, Cat. No. C0101137</p>
<p></p>
<p><strong>Storage conditions: </strong>Store at -20°C. Guaranteed stable for 6 months from date of receipt when stored properly.</p>
<p><strong>Storage buffer:</strong> Supplied in solution containing 50% v/v glycerol.</p>
<p><strong>Properties & Usage: </strong>Tagmentase is dependent on Mg++ for activity. Avoid chelators, such as EDTA/EGTA, in reaction buffers. The enzyme is active at pH 7.5-8 at 37-55°C. SDS, EDTA/EGTA or heating to 65°C will inactivate the enzyme.</p>
<p><strong>Applications:</strong> Tagmentase (Tn5 transposase) – loaded can be used in a variety of applications to construct library for second-generation sequencing. Please note that an additional optimization might be required for custom protocols including the enzyme dose- and time-response experiments.</p>
<div>For ATAC-seq and ChIPmentation, we recommend using Diagenode validated protocols:</div>
<div>ATAC-seq kit, Cat. No. C01080002</div>
<div>ATAC-seq package for tissue, Cat. No. <span>C01080006</span></div>
<div>ChIPmentation Kit for Histones, Cat. No. C01011009</div>
<div>µChIPmentation Kit for Histones, Cat. No. C01011011</div>
<div>TAG Kit for ChIPmentation, Cat. No. C01011030</div>
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<p>The Diagenode <strong>µChIPmentation Kit for Histones</strong> is optimized to perform ChIP-seq on as little as <strong>10.000 cells</strong> from cell fixation to purified libraries. To <strong>reduce DNA lost</strong> the number of sample transfer from tube to tube has been limited: during whole workflow - from cell fixation to library prurification - only 3 tubes per sample are used. Reduced number of steps, reduced number of sample transfer and <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation" target="_blank">ChIPmentation technology</a> itself enable for <strong>efficient and robust ChIP-seq</strong> on <strong>limited amount of sample</strong>. The kit µChIPmentation for Histones includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The primer indexes for multiplexing are not included in the kit and have to be purchase separately - <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">read more</a>.</p>
<div class="extra-spaced">
<h3>Benefits of the µChIPmentation system for histone ChIP-seq</h3>
<ul>
<li><strong>Easier</strong> and <strong>faster</strong> than classical ChIP-seq</li>
<li>Optimized for <strong>low input samples</strong>: as little as 10,000 cells</li>
<li>Protocol optimized for <strong>FACS-sorted cells</strong></li>
<li>Validated for various <strong>histone marks</strong></li>
<li><strong>High quality</strong> sequencing data</li>
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'info1' => '<p>ChIPmentation is based on tagmentation that allows library preparation to be integrated during the ChIP itself using transposase and sequencing-compatible adaptors. Our new improved µChIPmentation protocol combines 3 features for guaranteeing high quality sequencing data on small sample inputs 1) optimized chromatin shearing preparation protocol, 2) reduced number of steps, 3) reduced number of sample transfer from tube to tube - only 3 tubes per sample for the whole process, from cell fixation to purified libraries.</p>
<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"><i class="fa fa-caret-right"></i> Workflow of µChIPmentation for Histones - Read more</a>
<div id="workflow" class="content">
<div class="extra-spaced"><center><img src="https://www.diagenode.com/img/product/kits/workflow-micro-chipmentation.png" /></center></div>
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<div class="small-12 medium-12 large-12 columns"><strong>A.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1A.png" /></div>
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<div class="small-12 medium-5 large-5 columns" columns=""><strong>B.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1B.png" /></div>
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<p style="text-align: left;"><strong>Figure 1. Comparison between ChIPmentation and µChIPmentation </strong><br />ChIPmentation: chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011000) and diluted chromatin from 500.000 cells was used for the immunoprecipitation. µChIPmentation: chromatin preparation and immunoprecipitation have been performed on 10.000 K562 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032). The Diagenode antibody targeting H3K27me3 (Cat. No. C15410195) was used.<br /> A. Distribution of the ChIPmentation and µChIPmentation readsets in a representative region of the genome (in duplicates). B. Comparison of the top 40% peaks from µChIPmentation (10.000 cells) with dataset generated with ChIPmentation (500.000 cells).</p>
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<p><strong>Figure 2. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
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<p><strong>Figure 3. Integrative genomics viewer (IGV) visualization of from ChIP-seq experiments using H3K27me3 antibody (Diagenode, Cat. No. <span>C15410195</span>) and 10.000 cells of K562 cells per immunoprecipitation.<br /></strong>Cells were FACS-sorted and ChIP has been performed accordingly to µChIPmentation protocol. Batch chromatin preparation followed by immunoprecipitation has been used per comparison as indicated.</p>
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<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
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<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries" target="_blank">8 SI for Tagmented libraries Cat. No. C01011033</a></li>
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<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for Tagmented libraries, Cat. No. C0101135</a></li>
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<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
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<p style="text-align: justify;">Diagenode’s epigenetic reagents include:</p>
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<li style="text-align: justify;"><strong>DNA methylation kits and antibodies</strong> - Validated NGS-compatible kits for MeDIP, MBD pull-down, whole genome bisulfite sequencing, and reduced representation bisulfite sequencing. Official provider for the original clone for 5-mC 33D3.</li>
<li style="text-align: justify;"><strong>ChIP and ChIP-seq kits for industry-leading specificity and sensitivity</strong> - MicroChIP/MicroPlex Kit for ChIP-seq with only 10,000 cells and the iDeal ChIP-seq Kits optimized for both transcription factors and histones. Our kits feature full reagents for ChIP-seq including control primers, control antibodies, magnetics beads, and purification reagents.</li>
<li style="text-align: justify;"><strong>Library preparation kits</strong> tailored for your specific requirements. The MicroPlex Library Preparation Kit simplifies library preparation requiring only 3 simple steps and allowing inputs of only 50 pg. </li>
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'description' => '<p><span>Non-small cell lung cancer (NSCLC) is the deadliest cancer worldwide. Therapeutic progress stagnate, highlighting the complexity to replicate NSCLC in preclinical models. Drug discovery studies rely mostly on cancer cells in two-dimension (2D), which poorly predict drug efficacy in patients. There is a growing interest in three-dimensional (3D) preclinical models, such as 3D spheroids, to better model tumor phenotype and improve therapeutic prediction. However, a comprehensive view of 3D culture methods impact on transcriptomes, epigenomes and pharmacological responses and their correlations to NSCLC tumors is still missing. Here, we demonstrate that NSCLC spheroids undergo time-dependent transcriptomic and epigenomic changes, which peak after 3 weeks of culture. While DNA methylome remained stable, chromatin methylation and acetylation marks gained features of advanced NSCLC in a time-dependent manner. Single-cell transcriptomic profiling of spheroids demonstrated that time of 3D culture improved the correlation to NSCLC tumors. Moreover, long-term culture of 3D spheroids increased drug screening predictability, by showing resistance to drugs that failed in NSCLC patients (such as HDAC inhibitors) while demonstrating novel pharmacological vulnerabilities and synergistic interactions (such as combination of PRMT and HDAC inhibitors). Strikingly, reverting 3D spheroids back to 2D culture rapidly reversed transcriptomic, epigenetic and pharmacological signatures acquired after 3 weeks of 3D culture, highlighting the critical impact of cell culture conditions on NSCLC phenotype. Collectively, our findings demonstrate that implementing a time-dependent maturation process into 3D spheroid culture induces chromatin and transcriptomic changes that enhance NSCLC preclinical modeling.</span></p>',
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'description' => '<p><b>Background:</b><span><span> </span>Environmental factors make an important contribution to suicide. Histone tails are prone to different modifications, leading to changes of chromatin (de)condensation and consequently gene expression.<span> </span></span><b>Materials & methods:</b><span><span> </span>Level of H3K14ac was studied with chromatin immunoprecipitation followed by high-throughput DNA sequencing. Genes were further validated with RT-qPCR; using hippocampal tissue.<span> </span></span><b>Results:</b><span><span> </span>We showed lowered H3K14ac levels in individuals who died by suicide. The genes<span> </span></span><i>ADORA2A</i><span>,<span> </span></span><i>B4GALT2</i><span><span> </span>and<span> </span></span><i>MMP14</i><span><span> </span>showed differential expression in individuals who died by suicide. Identified genetic and protein interactions among genes show interactions with suicide-related genes.<span> </span></span><b>Conclusion:</b><span><span> </span>Further investigations of histone modifications in association with DNA methylation and miRNA are needed to expand our knowledge of the genes that could significantly contribute to suicide.</span></p>',
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'description' => '<p>The rhesus macaque provides a unique model of acquired immunity against schistosomes, which afflict \>200 million people worldwide. By monitoring bloodstream levels of parasite-gut-derived antigen, we show that from week 10 onwards an established infection with Schistosoma mansoni is cleared in an exponential manner, eliciting resistance to reinfection. Secondary challenge at week 42 demonstrates that protection is strong in all animals and complete in some. Antibody profiles suggest that antigens mediating protection are the released products of developing schistosomula. In culture they are killed by addition of rhesus plasma, collected from week 8 post-infection onwards, and even more efficiently with post-challenge plasma. Furthermore, cultured schistosomula lose chromatin activating marks at the transcription start site of genes related to worm development and show decreased expression of genes related to lysosomes and lytic vacuoles involved with autophagy. Overall, our results indicate that enhanced antibody responses against the challenge migrating larvae mediate the naturally acquired protective immunity and will inform the route to an effective vaccine.</p>',
'date' => '2021-10-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34702841',
'doi' => '10.1038/s41467-021-26497-0',
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'description' => '<p>Tissue turnover requires activation and lineage commitment of tissue-resident stem cells (SCs). These processes are impacted by ageing, but the mechanisms remain unclear. Here, we addressed the mechanisms of ageing in murine hair follicle SCs (HFSCs) and observed a widespread reduction in chromatin accessibility in aged HFSCs, particularly at key self-renewal and differentiation genes, characterized by bivalent promoters occupied by active and repressive chromatin marks. Consistent with this, aged HFSCs showed reduced ability to activate bivalent genes for efficient self-renewal and differentiation. These defects were niche dependent as the transplantation of aged HFSCs into young recipients or synthetic niches restored SC functions. Mechanistically, the aged HFSC niche displayed widespread alterations in extracellular matrix composition and mechanics, resulting in mechanical stress and concomitant transcriptional repression to silence promoters. As a consequence, increasing basement membrane stiffness recapitulated age-related SC changes. These data identify niche mechanics as a central regulator of chromatin state, which, when altered, leads to age-dependent SC exhaustion.</p>',
'date' => '2021-07-01',
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'name' => 'A Tumor Suppressor Enhancer of PTEN in T-cell development and leukemia',
'authors' => 'L. Tottone at al.',
'description' => '<p>Long-range oncogenic enhancers play an important role in cancer. Yet, whether similar regulation of tumor suppressor genes is relevant remains unclear. Loss of expression of PTEN is associated with the pathogenesis of various cancers, including T-cell leukemia (T-ALL). Here, we identify a highly conserved distal enhancer (PE) that interacts with the <em>PTEN</em> promoter in multiple hematopoietic populations, including T-cells, and acts as a hub of relevant transcription factors in T-ALL. Consistently, loss of PE leads to reduced <em>PTEN</em> levels in T-ALL cells. Moreover, PE-null mice show reduced <em>Pten</em> levels in thymocytes and accelerated development of NOTCH1-induced T-ALL. Furthermore, secondary loss of PE in established leukemias leads to accelerated progression and a gene expression signature driven by <em>Pten</em> loss. Finally, we uncovered recurrent deletions encompassing PE in T-ALL, which are associated with decreased <em>PTEN</em> levels. Altogether, our results identify PE as the first long-range tumor suppressor enhancer directly implicated in cancer.</p>',
'date' => '2021-01-01',
'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/33458694/',
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'name' => 'Epigenetic effects of low-level sodium arsenite exposure on human liver HepaRG cells',
'authors' => 'Tryndyak, V.P., Borowa-Mazgaj, B., Steward, C.R. et al. Epigenetic effects of low-level sodium arsenite exposure on human liver HepaRG cells.',
'description' => '<p>Chronic exposure to inorganic arsenic is associated with a variety of adverse health effects, including lung, bladder, kidney, and liver cancer. Several mechanisms have been proposed for arsenic-induced tumorigenesis; however, insufficient knowledge and many unanswered questions remain to explain the integrated molecular pathogenesis of arsenic carcinogenicity. In the present study, using non-tumorigenic human liver HepaRG cells, we investigated epigenetic alterations upon prolonged exposure to a noncytotoxic concentration of sodium arsenite (NaAsO<sub>2</sub>). We demonstrate that continuous exposure of HepaRG cells to 1 µM sodium arsenite (NaAsO<sub>2</sub>) for 14 days resulted in substantial cytosine DNA demethylation and hypermethylation across the genome, among which the claudin 14 (<i>CLDN14</i>) gene was hypermethylated and the most down-regulated gene. Another important finding was a profound loss of histone H3 lysine 36 (H3K36) trimethylation, which was accompanied by increased damage to genomic DNA and an elevated de novo mutation frequency. These results demonstrate that continuous exposure of HepaRG cells to a noncytotoxic concentration of NaAsO<sub>2</sub> results in substantial epigenetic abnormalities accompanied by several carcinogenesis-related events, including induction of epithelial-to-mesenchymal transition, damage to DNA, inhibition of DNA repair genes, and induction of de novo mutations. Importantly, this study highlights the intimate mechanistic link and interplay between two fundamental cancer-associated events, epigenetic and genetic alterations, in arsenic-associated carcinogenesis.</p>',
'date' => '2020-08-25',
'pmid' => 'https://link.springer.com/article/10.1007/s00204-020-02872-6',
'doi' => 'https://doi.org/10.1007/s00204-020-02872-6',
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'name' => 'Distinct and temporary-restricted epigenetic mechanisms regulate human αβ and γδ T cell development ',
'authors' => 'Roels J, Kuchmiy A, De Decker M, et al. ',
'description' => '<p>The development of TCRαβ and TCRγδ T cells comprises a step-wise process in which regulatory events control differentiation and lineage outcome. To clarify these mechanisms, we employed RNA-sequencing, ATAC-sequencing and ChIPmentation on well-defined thymocyte subsets that represent the continuum of human T cell development. The chromatin accessibility dynamics show clear stage specificity and reveal that human T cell-lineage commitment is marked by GATA3- and BCL11B-dependent closing of PU.1 sites. A temporary increase in H3K27me3 without open chromatin modifications is unique for β-selection, whereas emerging γδ T cells, which originate from common precursors of β-selected cells, show large chromatin accessibility changes due to strong T cell receptor (TCR) signaling. Furthermore, we unravel distinct chromatin landscapes between CD4<sup>+</sup> and CD8<sup>+</sup> αβ-lineage cells that support their effector functions and reveal gene-specific mechanisms that define mature T cells. This resource provides a framework for studying gene regulatory mechanisms that drive normal and malignant human T cell development.</p>',
'date' => '2020-07-27',
'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/32719521/',
'doi' => ' 10.1038/s41590-020-0747-9 ',
'modified' => '2021-01-29 14:12:02',
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'name' => 'Measuring Histone Modifications in the Human Parasite Schistosoma mansoni ',
'authors' => 'de Carvalho Augusto R, Roquis D, Al Picard M, Chaparro C, Cosseau C, Grunau C.',
'description' => '<p>DNA-binding proteins play critical roles in many major processes such as development and sexual biology of Schistosoma mansoni and are important for the pathogenesis of schistosomiasis. Chromatin immunoprecipitation (ChIP) experiments followed by sequencing (ChIP-seq) are useful to characterize the association of genomic regions with posttranslational chemical modifications of histone proteins. Challenges in the standard ChIP protocol have motivated recent enhancements in this approach, such as reducing the number of cells required and increasing the resolution. In this chapter, we describe the latest advances made by our group in the ChIP methods to improve the standard ChIP protocol to reduce the number of input cells required and to increase the resolution and robustness of ChIP in S. mansoni.</p>',
'date' => '2020-05-26',
'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/32451999/',
'doi' => '10.1007/978-1-0716-0635-3_9 ',
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<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Tagmentase (Tn5 transposase) - loaded</strong> to my shopping cart.</p>
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'
$related = array(
'id' => '3083',
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'name' => 'µChIPmentation Kit for Histones',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/microchipmentation-for-histones.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The Diagenode <strong>µChIPmentation Kit for Histones</strong> is optimized to perform ChIP-seq on as little as <strong>10.000 cells</strong> from cell fixation to purified libraries. To <strong>reduce DNA lost</strong> the number of sample transfer from tube to tube has been limited: during whole workflow - from cell fixation to library prurification - only 3 tubes per sample are used. Reduced number of steps, reduced number of sample transfer and <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation" target="_blank">ChIPmentation technology</a> itself enable for <strong>efficient and robust ChIP-seq</strong> on <strong>limited amount of sample</strong>. The kit µChIPmentation for Histones includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The primer indexes for multiplexing are not included in the kit and have to be purchase separately - <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">read more</a>.</p>
<div class="extra-spaced">
<h3>Benefits of the µChIPmentation system for histone ChIP-seq</h3>
<ul>
<li><strong>Easier</strong> and <strong>faster</strong> than classical ChIP-seq</li>
<li>Optimized for <strong>low input samples</strong>: as little as 10,000 cells</li>
<li>Protocol optimized for <strong>FACS-sorted cells</strong></li>
<li>Validated for various <strong>histone marks</strong></li>
<li><strong>High quality</strong> sequencing data</li>
</ul>
</div>',
'label1' => 'Characteristics',
'info1' => '<p>ChIPmentation is based on tagmentation that allows library preparation to be integrated during the ChIP itself using transposase and sequencing-compatible adaptors. Our new improved µChIPmentation protocol combines 3 features for guaranteeing high quality sequencing data on small sample inputs 1) optimized chromatin shearing preparation protocol, 2) reduced number of steps, 3) reduced number of sample transfer from tube to tube - only 3 tubes per sample for the whole process, from cell fixation to purified libraries.</p>
<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"><i class="fa fa-caret-right"></i> Workflow of µChIPmentation for Histones - Read more</a>
<div id="workflow" class="content">
<div class="extra-spaced"><center><img src="https://www.diagenode.com/img/product/kits/workflow-micro-chipmentation.png" /></center></div>
</div>
</li>
</ul>
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<div class="small-12 medium-12 large-12 columns"><strong>A.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1A.png" /></div>
</div>
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<div class="small-12 medium-5 large-5 columns" columns=""><strong>B.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1B.png" /></div>
<div class="small-12 medium-7 large-7 columns">
<p style="text-align: left;"><strong>Figure 1. Comparison between ChIPmentation and µChIPmentation </strong><br />ChIPmentation: chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011000) and diluted chromatin from 500.000 cells was used for the immunoprecipitation. µChIPmentation: chromatin preparation and immunoprecipitation have been performed on 10.000 K562 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032). The Diagenode antibody targeting H3K27me3 (Cat. No. C15410195) was used.<br /> A. Distribution of the ChIPmentation and µChIPmentation readsets in a representative region of the genome (in duplicates). B. Comparison of the top 40% peaks from µChIPmentation (10.000 cells) with dataset generated with ChIPmentation (500.000 cells).</p>
</div>
</div>
<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig2.png" /></div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 2. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
</div>
</div>
</div>
<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig3.png" /></div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 3. Integrative genomics viewer (IGV) visualization of from ChIP-seq experiments using H3K27me3 antibody (Diagenode, Cat. No. <span>C15410195</span>) and 10.000 cells of K562 cells per immunoprecipitation.<br /></strong>Cells were FACS-sorted and ChIP has been performed accordingly to µChIPmentation protocol. Batch chromatin preparation followed by immunoprecipitation has been used per comparison as indicated.</p>
</div>
</div>
</div>',
'label2' => 'Additional solutions for µChIPmentation Kit for Histones',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit - High SDS</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for multiplexing:</p>
<ul class="no-bullet">
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries" target="_blank">24 SI for Tagmented libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries" target="_blank">8 SI for Tagmented libraries Cat. No. C01011033</a></li>
<li><em></em><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for Tagmented libraries, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
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<p><b>ChIPmentation</b> is a method that combines <b>chromatin </b><b>immunoprecipiation</b> and <b>tagmentation</b><b>-based library preparation </b>using a fast and robust ChIP-seq protocol for studying <b>protein/DNA interactions</b>. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters. </p>
<p>The <b>ChIPmentation</b><b> Kit for Histones </b>includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The <b>primer indexes </b>for multiplexing are <b>not included</b> in the kit and have to be purchase separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">libraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
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<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
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<p><b>Benefits of the </b><b>ChIPmentation</b><b> system for histone </b><b>ChIP</b><b>-seq</b></p>
<ul>
<li>Easier and faster than classical ChIP-seq</li>
<li>Validated for various histone marks for a standard amount of cells</li>
<li>Generate high quality sequencing data</li>
</ul>
<p>For low input samples (10,000 cells) we recommend the <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation kit for Histones</a>.</p>
<p>For ChIP-seq on transcription factors we recommend the <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal</a> <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">ChIP-seq</a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns"> for transcription </a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">factors</a> + <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG kit for </a><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">ChIPmentation</a></p>',
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'info1' => '<p>The Diagenode ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.</p>
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<p><small><b>Figure 1: </b><b>ChIPmentation</b> <b>sequencing</b> <b>results</b> <b>obtained</b> <b>from</b> <b>decreasing</b> <b>starting</b> <b>amounts</b><b> of </b><b>cells</b><b>.<br /> </b><br /> Chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.</small></p>
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<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-5.png" /></p>
<p><small><b>Figure 2: </b><b>ChIPmentation</b><b> sequencing results.</b></small></p>
<p>Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).</p>',
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'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">iDeal</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells"> Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP</a><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for tagmenteted libraries:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">lilbraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
</ul>
<p>The kit ChIPmentation for Histones is validated on the <a href="https://www.diagenode.com/en/categories/ip-star">IP-Star Compact System </a>and the corresponding protocol is included in the manual.</p>',
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<p><b>ChIPmentation</b> is a method that combines <b>chromatin </b><b>immunoprecipiation</b> and <b>tagmentation</b><b>-based library preparation </b>using a fast and robust ChIP-seq protocol for studying <b>protein/DNA interactions</b>. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters. </p>
<p>The <b>ChIPmentation</b><b> Kit for Histones </b>includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The <b>primer indexes </b>for multiplexing are <b>not included</b> in the kit and have to be purchase separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">libraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
</ul>
<p><b>Benefits of the </b><b>ChIPmentation</b><b> system for histone </b><b>ChIP</b><b>-seq</b></p>
<ul>
<li>Easier and faster than classical ChIP-seq</li>
<li>Validated for various histone marks for a standard amount of cells</li>
<li>Generate high quality sequencing data</li>
</ul>
<p>For low input samples (10,000 cells) we recommend the <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation kit for Histones</a>.</p>
<p>For ChIP-seq on transcription factors we recommend the <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal</a> <a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">ChIP-seq</a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns"> for transcription </a><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">factors</a> + <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG kit for </a><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">ChIPmentation</a></p>',
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'info1' => '<p>The Diagenode ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.</p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-1.png" /></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-2.png" /></div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-3.png" /></div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-4.png" /></div>
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<p><small><b>Figure 1: </b><b>ChIPmentation</b> <b>sequencing</b> <b>results</b> <b>obtained</b> <b>from</b> <b>decreasing</b> <b>starting</b> <b>amounts</b><b> of </b><b>cells</b><b>.<br /> </b><br /> Chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.</small></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/ChIPmentation-for-histone-5.png" /></p>
<p><small><b>Figure 2: </b><b>ChIPmentation</b><b> sequencing results.</b></small></p>
<p>Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).</p>',
'label2' => 'Additional solutions compatible with ChIPmentation Kit for Histones ',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">iDeal</a><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells"> Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP</a><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for tagmenteted libraries:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">24 SI for </a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries"> libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">8 SI for </a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries">Tagmented</a><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries"> libraries Cat. No. C01011033</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for </a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Tagmented</a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"> libraries, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for </a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">Tagmented</a> <a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">lilbraries</a><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">, Cat. No. C0101135</a></li>
</ul>
<p>The kit ChIPmentation for Histones is validated on the <a href="https://www.diagenode.com/en/categories/ip-star">IP-Star Compact System </a>and the corresponding protocol is included in the manual.</p>',
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<p> </p>
<p>Diagenode <b>Tagmentase</b><b> – loaded </b>is a <strong>hyperactive Tn5 transposase</strong> preloaded with Illumina-compatible sequencing adapters. Its ability to cut DNA and insert the sequencing adapters in one step makes it the perfect companion for Next-Generation Sequencing experiments using powerful technologies such as ATAC-seq or ChIPmentation.</p>
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<p>Using Diagenode’s Tagmentase (Tn5 transposase) - loaded you may need also:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/tagmentation-buffer-1x-1ml">Tagmentation Buffer (1x)</a></li>
<li><a href="https://www.diagenode.com/en/p/tagmentation-buffer-2x">Tagmentation Buffer (2x)</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for tagmented libraries</a></li>
</ul>
<p>Looking for unloaded Tagmentase? Please go to <a href="https://www.diagenode.com/en/p/tagmentase-20-ul">Tagmentase</a><a href="https://www.diagenode.com/en/p/tagmentase-20-ul"> (Tn5 transposase) – </a><a href="https://www.diagenode.com/en/p/tagmentase-20-ul">unloaded</a></p>',
'label1' => 'Examples of results',
'info1' => '<p><b>Tagmentase</b><b> (Tn5 transposase) – </b><b>loaded</b> can be used in any application using tagmentation-based library preparation, like for example ChIPmentation, ATAC-seq. Here below we are showing some examples of results.</p>
<center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig1.png" width="700" alt="Tn5 transposase Enxzymes" caption="false" /></center>
<p><small><strong>Figure 1. Typical library profile of ATAC-seq library generated with the Tagmentase</strong><br /> ATAC-seq has been performed on 50,000 nuclei from K562 cells using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043). The library profile has been generated on a Fragment Analyzer (Agilent).</small></p>
<div class="row">
<div class="small-8 columns">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig2.png" width="200" alt="Diagenode Tagmentation " caption="false" /></center></div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Enrichments at TSS of ATAC-seq libraries generated with the Tagmentase</strong><br />ATAC-seq has been performed on 50,000 nuclei from K562 cells, in triplicates, using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043). Heatmaps around the hg19 TSS were generated using deeptools plotHeatmap functionality.</small></p>
</div>
</div>
</div>
<center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig3.png" alt="Transposase enzymes for ATAC-Seq" caption="false" width="956" height="259" /></center>
<p><small><strong>Figure 3. Sequencing profiles of ATAC-seq libraries generated with the Tagmentase </strong><br /> ATAC-seq has been performed on 50,000 nuclei from K562 cells, in triplicates, using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043).</small></p>
<center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig4.png" width="700" alt="purified Transposase enzymes " caption="false" /></center>
<p><small><strong>Figure 4. Typical library profile of ChIPmentation library generated with the Tagmentase</strong><br /> Chromatin preparation and immunoprecipitation have been performed on 1,000,000 K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011010) in combination with the Diagenode antibody targeting H3K4me3 (Cat. No. C15410003) and with the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012). The library profile has been generated on a Fragment Analyzer (Agilent).</small></p>
<center><img src="https://www.diagenode.com/img/product/kits/tagmentase-loaded-fig5.png" alt="pA-Tn5 Transposase Enzymes" caption="false" width="956" height="262" /></center>
<p><small><strong>Figure 5. Sequencing profiles of ChIPmentation libraries generated with the Tagmentase</strong><br /> Chromatin preparation and immunoprecipitation have been performed on 500.000 to 1,000,000 cells using the <a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones </a>(Cat. No. C01011010) in combination with the <a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Tagmentase (Tn5 transposase) – loaded </a>(Cat. No. C01070012) on K562 cells. The Diagenode antibodies targeting <a href="https://www.diagenode.com/en/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">H3K4me3 </a>(Cat. No. C15410003), <a href="https://www.diagenode.com/en/p/h3k27ac-polyclonal-antibody-premium-50-mg-18-ml">H3K27ac </a>(Cat. No. C15410196), <a href="https://www.diagenode.com/en/p/h3k36me3-polyclonal-antibody-premium-50-mg">H3K36me3 </a>(Cat. No. C15410192), <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">H3K27me3</a> (Cat. No. C15410195) and rabbit <a href="https://www.diagenode.com/en/p/rabbit-igg-250-ug-250-ul">IgG </a>(Cat. No. C15410206) have been used.</small></p>
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'label2' => 'Product information',
'info2' => '<p><strong>Tagmentase (Tn5 transposase) - loaded </strong></p>
<div>Protein Molecular weight: 53.3 kDa</div>
<p>Expressed: in Escherichia coli</p>
<p></p>
<p><strong>Product description:</strong> Diagenode Tagmentase – loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters. Its ability to cut DNA and insert the sequencing adapters in one step makes it the perfect companion for Next-Generation Sequencing experiments. The Tagmentase is pre-loaded with sequencing adapters compatible with Illumina Nextera platforms, as shown below. The oligos loaded on the Tagmentase are inserted into DNA upon a tagmentation reaction.</p>
<div>Mosaic end_reverse: 5’ [PHO]<span style="text-decoration: underline;">CTGTCTCTTATACACATCT</span> 3’</div>
<div>Mosaic end_Adapter A: 5’ TCGTCGGCAGCGTC<span style="text-decoration: underline;">AGATGTGTATAAGAGACAG</span> 3’</div>
<p>Mosaic end_Adapter B: 5’ GTCTCGTGGGCTCGG<span style="text-decoration: underline;">AGATGTGTATAAGAGACAG</span> 3’</p>
<p></p>
<div><span style="text-decoration: underline;">Underlined regions</span> correspond to the double-stranded part of the adapter, recognized by the Tagmentase. The final libraries can be amplified using Diagenode Primer indexes for tagmented libraries:</div>
<div>24 SI for tagmented libraries, Cat. No. C01011032</div>
<div>24 UDI fortagmented libraries - Set I, Cat. No. C0101134</div>
<div>24 UDI for tagmented libraries - Set II, Cat. No. C0101136</div>
<p>24 UDI for tagmented libraries - Set III, Cat. No. C0101137</p>
<p></p>
<p><strong>Storage conditions: </strong>Store at -20°C. Guaranteed stable for 6 months from date of receipt when stored properly.</p>
<p><strong>Storage buffer:</strong> Supplied in solution containing 50% v/v glycerol.</p>
<p><strong>Properties & Usage: </strong>Tagmentase is dependent on Mg++ for activity. Avoid chelators, such as EDTA/EGTA, in reaction buffers. The enzyme is active at pH 7.5-8 at 37-55°C. SDS, EDTA/EGTA or heating to 65°C will inactivate the enzyme.</p>
<p><strong>Applications:</strong> Tagmentase (Tn5 transposase) – loaded can be used in a variety of applications to construct library for second-generation sequencing. Please note that an additional optimization might be required for custom protocols including the enzyme dose- and time-response experiments.</p>
<div>For ATAC-seq and ChIPmentation, we recommend using Diagenode validated protocols:</div>
<div>ATAC-seq kit, Cat. No. C01080002</div>
<div>ATAC-seq package for tissue, Cat. No. <span>C01080006</span></div>
<div>ChIPmentation Kit for Histones, Cat. No. C01011009</div>
<div>µChIPmentation Kit for Histones, Cat. No. C01011011</div>
<div>TAG Kit for ChIPmentation, Cat. No. C01011030</div>
<p></p>
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[maximum depth reached]
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'name' => 'µChIPmentation Kit for Histones',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/microchipmentation-for-histones.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The Diagenode <strong>µChIPmentation Kit for Histones</strong> is optimized to perform ChIP-seq on as little as <strong>10.000 cells</strong> from cell fixation to purified libraries. To <strong>reduce DNA lost</strong> the number of sample transfer from tube to tube has been limited: during whole workflow - from cell fixation to library prurification - only 3 tubes per sample are used. Reduced number of steps, reduced number of sample transfer and <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation" target="_blank">ChIPmentation technology</a> itself enable for <strong>efficient and robust ChIP-seq</strong> on <strong>limited amount of sample</strong>. The kit µChIPmentation for Histones includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The primer indexes for multiplexing are not included in the kit and have to be purchase separately - <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">read more</a>.</p>
<div class="extra-spaced">
<h3>Benefits of the µChIPmentation system for histone ChIP-seq</h3>
<ul>
<li><strong>Easier</strong> and <strong>faster</strong> than classical ChIP-seq</li>
<li>Optimized for <strong>low input samples</strong>: as little as 10,000 cells</li>
<li>Protocol optimized for <strong>FACS-sorted cells</strong></li>
<li>Validated for various <strong>histone marks</strong></li>
<li><strong>High quality</strong> sequencing data</li>
</ul>
</div>',
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'info1' => '<p>ChIPmentation is based on tagmentation that allows library preparation to be integrated during the ChIP itself using transposase and sequencing-compatible adaptors. Our new improved µChIPmentation protocol combines 3 features for guaranteeing high quality sequencing data on small sample inputs 1) optimized chromatin shearing preparation protocol, 2) reduced number of steps, 3) reduced number of sample transfer from tube to tube - only 3 tubes per sample for the whole process, from cell fixation to purified libraries.</p>
<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"><i class="fa fa-caret-right"></i> Workflow of µChIPmentation for Histones - Read more</a>
<div id="workflow" class="content">
<div class="extra-spaced"><center><img src="https://www.diagenode.com/img/product/kits/workflow-micro-chipmentation.png" /></center></div>
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<div class="small-12 medium-12 large-12 columns"><strong>A.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1A.png" /></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-5 large-5 columns" columns=""><strong>B.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1B.png" /></div>
<div class="small-12 medium-7 large-7 columns">
<p style="text-align: left;"><strong>Figure 1. Comparison between ChIPmentation and µChIPmentation </strong><br />ChIPmentation: chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011000) and diluted chromatin from 500.000 cells was used for the immunoprecipitation. µChIPmentation: chromatin preparation and immunoprecipitation have been performed on 10.000 K562 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032). The Diagenode antibody targeting H3K27me3 (Cat. No. C15410195) was used.<br /> A. Distribution of the ChIPmentation and µChIPmentation readsets in a representative region of the genome (in duplicates). B. Comparison of the top 40% peaks from µChIPmentation (10.000 cells) with dataset generated with ChIPmentation (500.000 cells).</p>
</div>
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<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig2.png" /></div>
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<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 2. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
</div>
</div>
</div>
<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig3.png" /></div>
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<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 3. Integrative genomics viewer (IGV) visualization of from ChIP-seq experiments using H3K27me3 antibody (Diagenode, Cat. No. <span>C15410195</span>) and 10.000 cells of K562 cells per immunoprecipitation.<br /></strong>Cells were FACS-sorted and ChIP has been performed accordingly to µChIPmentation protocol. Batch chromatin preparation followed by immunoprecipitation has been used per comparison as indicated.</p>
</div>
</div>
</div>',
'label2' => 'Additional solutions for µChIPmentation Kit for Histones',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit - High SDS</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for multiplexing:</p>
<ul class="no-bullet">
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries" target="_blank">24 SI for Tagmented libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries" target="_blank">8 SI for Tagmented libraries Cat. No. C01011033</a></li>
<li><em></em><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for Tagmented libraries, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
<li></li>
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<p style="text-align: justify;">Diagenode’s epigenetic reagents include:</p>
<ul>
<li style="text-align: justify;"><strong>DNA methylation kits and antibodies</strong> - Validated NGS-compatible kits for MeDIP, MBD pull-down, whole genome bisulfite sequencing, and reduced representation bisulfite sequencing. Official provider for the original clone for 5-mC 33D3.</li>
<li style="text-align: justify;"><strong>ChIP and ChIP-seq kits for industry-leading specificity and sensitivity</strong> - MicroChIP/MicroPlex Kit for ChIP-seq with only 10,000 cells and the iDeal ChIP-seq Kits optimized for both transcription factors and histones. Our kits feature full reagents for ChIP-seq including control primers, control antibodies, magnetics beads, and purification reagents.</li>
<li style="text-align: justify;"><strong>Library preparation kits</strong> tailored for your specific requirements. The MicroPlex Library Preparation Kit simplifies library preparation requiring only 3 simple steps and allowing inputs of only 50 pg. </li>
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'description' => '<p><b>Background:</b><span><span> </span>Environmental factors make an important contribution to suicide. Histone tails are prone to different modifications, leading to changes of chromatin (de)condensation and consequently gene expression.<span> </span></span><b>Materials & methods:</b><span><span> </span>Level of H3K14ac was studied with chromatin immunoprecipitation followed by high-throughput DNA sequencing. Genes were further validated with RT-qPCR; using hippocampal tissue.<span> </span></span><b>Results:</b><span><span> </span>We showed lowered H3K14ac levels in individuals who died by suicide. The genes<span> </span></span><i>ADORA2A</i><span>,<span> </span></span><i>B4GALT2</i><span><span> </span>and<span> </span></span><i>MMP14</i><span><span> </span>showed differential expression in individuals who died by suicide. Identified genetic and protein interactions among genes show interactions with suicide-related genes.<span> </span></span><b>Conclusion:</b><span><span> </span>Further investigations of histone modifications in association with DNA methylation and miRNA are needed to expand our knowledge of the genes that could significantly contribute to suicide.</span></p>',
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<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> µChIPmentation Kit for Histones</strong> to my shopping cart.</p>
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<p>The Diagenode <strong>µChIPmentation Kit for Histones</strong> is optimized to perform ChIP-seq on as little as <strong>10.000 cells</strong> from cell fixation to purified libraries. To <strong>reduce DNA lost</strong> the number of sample transfer from tube to tube has been limited: during whole workflow - from cell fixation to library prurification - only 3 tubes per sample are used. Reduced number of steps, reduced number of sample transfer and <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation" target="_blank">ChIPmentation technology</a> itself enable for <strong>efficient and robust ChIP-seq</strong> on <strong>limited amount of sample</strong>. The kit µChIPmentation for Histones includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The primer indexes for multiplexing are not included in the kit and have to be purchase separately - <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">read more</a>.</p>
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<h3>Benefits of the µChIPmentation system for histone ChIP-seq</h3>
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<li><strong>Easier</strong> and <strong>faster</strong> than classical ChIP-seq</li>
<li>Optimized for <strong>low input samples</strong>: as little as 10,000 cells</li>
<li>Protocol optimized for <strong>FACS-sorted cells</strong></li>
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'info1' => '<p>ChIPmentation is based on tagmentation that allows library preparation to be integrated during the ChIP itself using transposase and sequencing-compatible adaptors. Our new improved µChIPmentation protocol combines 3 features for guaranteeing high quality sequencing data on small sample inputs 1) optimized chromatin shearing preparation protocol, 2) reduced number of steps, 3) reduced number of sample transfer from tube to tube - only 3 tubes per sample for the whole process, from cell fixation to purified libraries.</p>
<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"><i class="fa fa-caret-right"></i> Workflow of µChIPmentation for Histones - Read more</a>
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<p style="text-align: left;"><strong>Figure 1. Comparison between ChIPmentation and µChIPmentation </strong><br />ChIPmentation: chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011000) and diluted chromatin from 500.000 cells was used for the immunoprecipitation. µChIPmentation: chromatin preparation and immunoprecipitation have been performed on 10.000 K562 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032). The Diagenode antibody targeting H3K27me3 (Cat. No. C15410195) was used.<br /> A. Distribution of the ChIPmentation and µChIPmentation readsets in a representative region of the genome (in duplicates). B. Comparison of the top 40% peaks from µChIPmentation (10.000 cells) with dataset generated with ChIPmentation (500.000 cells).</p>
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<p><strong>Figure 2. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
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<p><strong>Figure 3. Integrative genomics viewer (IGV) visualization of from ChIP-seq experiments using H3K27me3 antibody (Diagenode, Cat. No. <span>C15410195</span>) and 10.000 cells of K562 cells per immunoprecipitation.<br /></strong>Cells were FACS-sorted and ChIP has been performed accordingly to µChIPmentation protocol. Batch chromatin preparation followed by immunoprecipitation has been used per comparison as indicated.</p>
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'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit - High SDS</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
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<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for Tagmented libraries, Cat. No. C0101135</a></li>
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<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
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'description' => '<p>Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Two versions of protocol have been validated: a manual procedure and an automated protocol on the<span style="font-weight: 400;"> IP-Star Compact Automated System.</span></p>',
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