| D-Plex Total RNA-seq Kit MANUAL The Diagenode D-Plex Total RNA-seq Library Preparation kit is a tool designed for the study of th... | Download |
An updated version of this kit using D-Plex technology is available for superior performance.
| D-Plex Total RNA-seq Kit MANUAL The Diagenode D-Plex Total RNA-seq Library Preparation kit is a tool designed for the study of th... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: CATS RNA-seq Kit v2 x24 (Diagenode Cat# C05010041). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Directional high-throughput sequencing of RNAs without gene-specific primers. |
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Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p> <p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. <span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.</span></span></p> <p><span><span>D-Plex Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p> <ul> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C"><span>C05030023</span> - D-Plex Unique Dual Indexes for Illumina - Set C </a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span> - D-Plex Unique Dual Indexes for Illumina - Set D </a></li> </ul> <div class="extra-spaced" align="center"></div> <div class="row"> <div class="carrousel" style="background-position: center;"> <div class="slick"> <div> <h3>Greater sensitivity to detect novel transcripts</h3> <div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div> <div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"> <p></p> <p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p> </div> </div> <div> <h3>Highest diversity to facilitate biomarker discovery</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p> </div> </div> <div> <h3>Consistent expression profiling across a wide range of RNA inputs</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p> </div> </div> <div> <h3>Superior library complexity at low RNA inputs</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p> </div> </div> </div> </div> </div>', 'label1' => '', 'info1' => '', 'label2' => 'Indexes', 'info2' => '<p><span>Specific D-Plex indexes </span><span>were designed and validated to fit the D-Plex technology for Illumina sequencing and </span><span>are not included in the kit. They can be bought separately according to your needs. Please choose the format that suits you best among the compatible references to:</span></p> <ul> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C" title="D-Plex UDI Module - Set C" target="_blank"><span>C05030023</span> - D-Plex Unique Dual Indexes for Illumina - Set C</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span> - D-Plex Unique Dual Indexes for Illumina - Set D </a></li> </ul> <p></p> <p>The use of UDI is highly recommended to mitigate errors introduced by read misassignment, including index hopping frequently observed with patterned flow cells such as Illumina’s NovaSeq system.</p>', 'label3' => 'Data analysis', 'info3' => '<p><span>A specific bioinformatics pipeline has been developed to process the special sequences present in the D-Plex construct, namely the UMI, the A-tail, and the template switch motif. All guidelines and free softwares are shared in the user manual. Subject to the compatibility of D-Plex constructs, other specific pipelines can be used.</span></p> <p><img src="https://www.diagenode.com/img/product/kits/dplex/bioinfoPipe.png" alt="Small RNA seq Bioinformatics pipeline" width="925" height="196" /></p>', 'format' => '24 rxns', 'catalog_number' => 'C05030031', 'old_catalog_number' => '', 'sf_code' => 'C05030031-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '1475', 'price_USD' => '1475', 'price_GBP' => '1355', 'price_JPY' => '231060', 'price_CNY' => '', 'price_AUD' => '3688', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'D-Plex-Total-RNA-seq-Library-Prep-x24', 'meta_title' => 'D-Plex Total RNA-seq Library Prep Kit for Illumina | Diagenode ', 'meta_keywords' => 'RNA-seq kit, RNA-seq library preparation; low input RNA-seq, UMI RNA-seq, UDI RNA-seq, D-Plex, higher RNA sensitivity', 'meta_description' => 'RNA-seq library preparation for Illumina sequencing - Unique D-Plex technology - Optimized for ultra-low input (50 pg total RNA) - UMI reduce PCR biases - UDI detect index hopping - Compatibility with FFPE samples - User-friendly and fast protocol', 'modified' => '2023-11-16 09:09:25', 'created' => '2021-04-13 14:10:40', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '14', 'position' => '10', 'parent_id' => '3', 'name' => 'DNA/RNA library preparation', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><span style="font-weight: 400;">Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to </span><a href="../applications/dna-rna-shearing"><span style="font-weight: 400;">fragmented DNA or RNA</span></a><span style="font-weight: 400;"> prior to sequencing</span></p> <p><span style="font-weight: 400;">After input DNA has been fragmented, it is end-repaired and blunt-ended</span><span style="font-weight: 400;">. The next step is a A-tailing in which dAMP is added to the 3´ end of the blunt phosphorylated DNA fragments to prevent concatemerization and to allow the ligation of adaptors with complementary dT overhangs. In addition, barcoded adapters can be incorporated to facilitate multiplexing prior to or during amplification.</span></p> <center><img src="https://www.diagenode.com/img/categories/library-prep/flux.png" /></center> <p><span style="font-weight: 400;">Diagenode offers a comprehensive product portfolio for library preparation:<br /></span></p> <strong><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex RNA-seq Library Preparation Kits</a></strong><br /> <p><span style="font-weight: 400;">Diagenode’s new RNA-sequencing solutions utilize the innovative c</span><span style="font-weight: 400;">apture and a</span><span style="font-weight: 400;">mplification by t</span><span style="font-weight: 400;">ailing and s</span><span style="font-weight: 400;">witching”</span><span style="font-weight: 400;">, a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of RNA. </span><span style="font-weight: 400;"></span><a href="../categories/Library-preparation-for-RNA-seq">Learn more</a></p> <strong><a href="../categories/library-preparation-for-ChIP-seq">ChIP-seq and DNA sequencing library preparation solutions</a></strong><br /> <p><span style="font-weight: 400;">Our kits have been optimized for DNA library preparation used for next generation sequencing for a wide range of inputs. Using a simple three-step protocols, our</span><a href="http://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><span style="font-weight: 400;"> </span></a><span style="font-weight: 400;">kits are an optimal choice for library preparation from DNA inputs down to 50 pg. </span><a href="../categories/library-preparation-for-ChIP-seq">Learn more</a></p> <a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span><strong>Bioruptor Pico - short fragments</strong></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br /> <p><span style="font-weight: 400;"></span><span style="font-weight: 400;">Our well-cited Bioruptor Pico is the shearing device of choice for chromatin and DNA fragmentation. Obtain uniform and tight fragment distributions between 150bp -2kb. </span><a href="../p/bioruptor-pico-sonication-device">Learn more</a></p> <strong><a href="../p/megaruptor2-1-unit"><span href="../p/bioruptor-pico-sonication-device">Megaruptor</span>® - long fragments</a></strong><a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br /> <p><span style="font-weight: 400;"></span><span style="font-weight: 400;">The Megaruptor is designed to shear DNA from 3kb-75kb for long-read sequencing. <a href="../p/megaruptor2-1-unit">Learn more</a></span></p> <span href="../p/bioruptor-pico-sonication-device"></span><span style="font-weight: 400;"></span></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'library-preparation', 'meta_keywords' => 'Library preparation,Next Generation Sequencing,DNA fragments,MicroPlex Library,iDeal Library', 'meta_description' => 'Diagenode offers a comprehensive product portfolio for library preparation such as CATS RNA-seq Library Preparation Kits,ChIP-seq and DNA sequencing library preparation solutions', 'meta_title' => 'Library preparation for Next Generation Sequencing (NGS) | Diagenode', 'modified' => '2021-03-10 03:27:16', 'created' => '2014-08-16 07:47:56', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3', 'position' => '10', 'parent_id' => null, 'name' => '次世代シーケンシング', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns"> <h2 style="font-size: 22px;">DNA断片化、ライブラリー調製、自動化：NGSのワンストップショップ</h2> <table class="small-12 medium-12 large-12 columns"> <tbody> <tr> <th class="small-12 medium-12 large-12 columns"> <h4>1. 断片化装置を選択してください：150 bp〜75 kbの範囲でDNAを断片化します。</h4> </th> </tr> <tr style="background-color: #ffffff;"> <td class="small-12 medium-12 large-12 columns"></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-pico-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/bioruptor_pico.jpg" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/megaruptor2-1-unit"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010001_megaruptor2.jpg" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-one-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-profil.png" /></a></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns">5μlまで断片化：150 bp〜2 kb<br />NGS DNAライブラリー調製およびFFPE核酸抽出に最適で、</td> <td class="small-4 medium-4 large-4 columns">2 kb〜75 kbの範囲をできます。<br />メイトペアライブラリー調製および長いフラグメントDNAシーケンシングに最適で、この軽量デスクトップデバイスで</td> <td class="small-4 medium-4 large-4 columns">20または50μlの断片化が可能です。</td> </tr> </tbody> </table> <table class="small-12 medium-12 large-12 columns"> <tbody> <tr> <th class="small-8 medium-8 large-8 columns"> <h4>2. 最適化されたライブラリー調整キットを選択してください。</h4> </th> <th class="small-4 medium-4 large-4 columns"> <h4>3. ライブラリー前処理自動化を選択して、比類のないデータ再現性を実感</h4> </th> </tr> <tr style="background-color: #ffffff;"> <td class="small-12 medium-12 large-12 columns"></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns"><a href="../p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><img src="https://www.diagenode.com/img/product/kits/microPlex_library_preparation.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/ideal-library-preparation-kit-x24-incl-index-primer-set-1-24-rxns"><img src="https://www.diagenode.com/img/product/kits/box_kit.jpg" style="display: block; margin-left: auto; margin-right: auto;" height="173" width="250" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/sx-8g-ip-star-compact-automated-system-1-unit"><img src="https://www.diagenode.com/img/product/automation/B03000002%20_ipstar_compact.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">50pgの低入力：MicroPlex Library Preparation Kit</td> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">5ng以上：iDeal Library Preparation Kit</td> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">Achieve great NGS data easily</td> </tr> </tbody> </table> </div> </div> <blockquote> <div class="row"> <div class="small-12 medium-12 large-12 columns"><span class="label" style="margin-bottom: 16px; margin-left: -22px; font-size: 15px;">DiagenodeがNGS研究にぴったりなプロバイダーである理由</span> <p>Diagenodeは15年以上もエピジェネティクス研究に専念、専門としています。 ChIP研究クロマチン用のユニークな断片化システムの開発から始まり、 専門知識を活かし、5μlのせん断体積まで可能で、NGS DNAライブラリーの調製に最適な最先端DNA断片化装置の開発にたどり着きました。 我々は以来、ChIP-seq、Methyl-seq、NGSライブラリー調製用キットを研究開発し、業界をリードする免疫沈降研究と同様に、ライブラリー調製を自動化および完結させる独自の自動化システムを開発にも成功しました。</p> <ul> <li>信頼されるせん断装置</li> <li>様々なインプットからのライブラリ作成キット</li> <li>独自の自動化デバイス</li> </ul> </div> </div> </blockquote> <div class="row"> <div class="small-12 columns"> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#panel1a">次世代シーケンシングへの理解とその専門知識</a> <div id="panel1a" class="content"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong>次世代シーケンシング (NGS)</strong> ）は、著しいスケールとハイスループットでシーケンシングを行い、1日に数十億もの塩基生成を可能にします。 NGSのハイスループットは迅速でありながら正確で、再現性のあるデータセットを実現し、さらにシーケンシング費用を削減します。 NGSは、ゲノムシーケンシング、ゲノム再シーケンシング、デノボシーケンシング、トランスクリプトームシーケンシング、その他にDNA-タンパク質相互作用の検出やエピゲノムなどを示します。 指数関数的に増加するシーケンシングデータの需要は、計算分析の障害や解釈、データストレージなどの課題を解決します。</p> <p>アプリケーションおよび出発物質に応じて、数百万から数十億の鋳型DNA分子を大規模に並行してシーケンシングすることが可能です。その為に、異なる化学物質を使用するいくつかの市販のNGSプラットフォームを利用することができます。 NGSプラットフォームの種類によっては、事前準備とライブラリー作成が必要です。</p> <p>NGSにとっても、特にデータ処理と分析に関した大きな課題はあります。第3世代技術はゲノミクス研究にさらに革命を起こすであろうと大きく期待されています。</p> </div> </div> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <p><strong>NGS アプリケーション</strong></p> <ul> <li>全ゲノム配列決定</li> <li>デノボシーケンシング</li> <li>標的配列</li> <li>Exomeシーケンシング</li> <li>トランスクリプトーム配列決定</li> <li>ゲノム配列決定</li> <li>ミトコンドリア配列決定</li> <li>DNA-タンパク質相互作用（ChIP-seq</li> <li>バリアント検出</li> <li>ゲノム仕上げ</li> </ul> </div> <div class="small-6 medium-6 large-6 columns"> <p><strong>研究分野におけるNGS:</strong></p> <ul> <li>腫瘍学</li> <li>リプロダクティブ・ヘルス</li> <li>法医学ゲノミクス</li> <li>アグリゲノミックス</li> <li>複雑な病気</li> <li>微生物ゲノミクス</li> <li>食品・環境ゲノミクス</li> <li>創薬ゲノミクス - パーソナライズド・メディカル</li> </ul> </div> <div class="small-12 medium-12 large-12 columns"> <p><strong>NGSの用語</strong></p> <dl> <dt>リード（読み取り）</dt> <dd>この装置から得られた連続した単一のストレッチ</dd> <dt>断片リード</dt> <dd>フラグメントライブラリからの読み込み。 シーケンシングプラットフォームに応じて、読み取りは通常約100〜300bp。</dd> <dt>断片ペアエンドリード</dt> <dd>断片ライブラリーからDNA断片の各末端2つの読み取り。</dd> <dt>メイトペアリード</dt> <dd>大きなDNA断片（通常は予め定義されたサイズ範囲）の各末端から2つの読み取り。</dd> <dt>カバレッジ（例）</dt> <dd>30×適用範囲とは、参照ゲノム中の各塩基対が平均30回の読み取りを示す。</dd> </dl> </div> </div> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h2>NGSプラットフォーム</h2> <h3><a href="http://www.illumina.com" target="_blank">イルミナ</a></h3> <p>イルミナは、クローン的に増幅された鋳型DNA（クラスター）上に位置する、蛍光標識された可逆的鎖ターミネーターヌクレオチドを用いた配列別合成技術を使用。 DNAクラスターは、ガラスフローセルの表面上に固定化され、 ワークフローは、4つのヌクレオチド（それぞれ異なる蛍光色素で標識された）の組み込み、4色イメージング、色素や末端基の切断、取り込み、イメージングなどを繰り返します。フローセルは大規模な並列配列決定を受ける。 この方法により、単一蛍光標識されたヌクレオチドの制御添加によるモノヌクレオチドのエラーを回避する可能性があります。 読み取りの長さは、通常約100〜150 bpです。</p> <h3><a href="http://www.lifetechnologies.com" target="_blank">イオン トレント</a></h3> <p>イオントレントは、半導体技術チップを用いて、合成中にヌクレオチドを取り込む際に放出されたプロトンを検出します。 これは、イオン球粒子と呼ばれるビーズの表面にエマルションPCR（emPCR）を使用し、リンクされた特定のアダプターを用いてDNA断片を増幅します。 各ビーズは1種類のDNA断片で覆われていて、異なるDNA断片を有するビーズは次いで、チップの陽子感知ウェル内に配置されます。 チップには一度に4つのヌクレオチドのうちの1つが浸水し、このプロセスは異なるヌクレオチドで15秒ごとに繰り返されます。 配列決定の間に4つの塩基の各々が1つずつ導入されます、組み込みの場合はプロトンが放出され、電圧信号が取り込みに比例して検出されます。.</p> <h3><a href="http://www.pacificbiosciences.com" target="_blank">パシフィック バイオサイエンス</a></h3> <p>パシフィックバイオサイエンスでは、20kbを超える塩基対の読み取りも、単一分子リアルタイム（SMRT）シーケンシングによる構造および細胞タイプの変化を観察することができます。 このプラットフォームでは、超長鎖二本鎖DNA（dsDNA）断片が、Megaruptor（登録商標）のようなDiagenode装置を用いたランダムシアリングまたは目的の標的領域の増幅によって生成されます。 SMRTbellライブラリーは、ユニバーサルヘアピンアダプターをDNA断片の各末端に連結することによって生成します。 サイズ選択条件による洗浄ステップの後、配列決定プライマーをSMRTbellテンプレートにアニーリングし、鋳型DNAに結合したDNAポリメラーゼを含む配列決定を、蛍光標識ヌクレオチドの存在下で開始。 各塩基が取り込まれると、異なる蛍光のパルスをリアルタイムで検出します。</p> <h3><a href="https://nanoporetech.com" target="_blank">オックスフォード ナノポア</a></h3> <p>Oxford Nanoporeは、単一のDNA分子配列決定に基づく技術を開発します。その技術により生物学的分子、すなわちDNAが一群の電気抵抗性高分子膜として位置するナノスケールの孔（ナノ細孔）またはその近くを通過し、イオン電流が変化します。 この変化に関する情報は、例えば4つのヌクレオチド（AまたはG r CまたはT）ならびに修飾されたヌクレオチドすべてを区別することによって分子情報に訳されます。 シーケンシングミニオンデバイスのフローセルは、数百個のナノポアチャネルのセンサアレイを含みます。 DNAサンプルは、Diagenode社のMegaruptor（登録商標）を用いてランダムシアリングによって生成され得る超長鎖DNAフラグメントが必要です。</p> <h3><a href="http://www.lifetechnologies.com/be/en/home/life-science/sequencing/next-generation-sequencing/solid-next-generation-sequencing.html" target="_blank">SOLiD</a></h3> <p>SOLiDは、ユニークな化学作用により、何千という個々のDNA分子の同時配列決定を可能にします。 それは、アダプター対ライブラリーのフラグメントが適切で、せん断されたゲノムDNAへのアダプターのライゲーションによるライブラリー作製から始まります。 次のステップでは、エマルジョンPCR（emPCR）を実施して、ビーズの表面上の個々の鋳型DNA分子をクローン的に増幅。 emPCRでは、個々の鋳型DNAをPCR試薬と混合し、水中油型エマルジョン内の疎水性シェルで囲まれた水性液滴内のプライマーコートビーズを、配列決定のためにロードするスライドガラスの表面にランダムに付着。 この技術は、シークエンシングプライマーへのライゲーションで競合する4つの蛍光標識されたジ塩基プローブのセットを使用します。</p> <h3><a href="http://454.com/products/technology.asp" target="_blank">454</a></h3> <p>454は、大規模並列パイロシーケンシングを利用しています。 始めに全ゲノムDNAまたは標的遺伝子断片の300〜800bp断片のライブラリー調製します。 次に、DNAフラグメントへのアダプターの付着および単一のDNA鎖の分離。 その後アダプターに連結されたDNAフラグメントをエマルジョンベースのクローン増幅（emPCR）で処理し、DNAライブラリーフラグメントをミクロンサイズのビーズ上に配置します。 各DNA結合ビーズを光ファイバーチップ上のウェルに入れ、器具に挿入します。 4つのDNAヌクレオチドは、配列決定操作中に固定された順序で連続して加えられ、並行して配列決定されます。</p> </div> </div> </div> </li> </ul> </div> </div>', 'in_footer' => true, 'in_menu' => true, 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The present kit incorporates the unique D-Plex technology to generate directional RNA libraries for Illumina sequencing directly from total RNAs, messenger RNAs (mRNAs) that have already been enriched by poly(A) capture, or RNAs that have already been depleted of ribosomal RNAs (rRNAs).</p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/dplex-total-rnaseq.pdf', 'slug' => 'dplex-total-rnaseq', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-04-20 14:09:33', 'created' => '2021-04-20 14:09:33', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1777', 'name' => 'product/kits/library-prep-kit-icon.png', 'alt' => 'Library prep kit icon', 'modified' => '2018-03-15 15:51:51', 'created' => '2018-03-15 15:51:26', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3401', 'name' => 'Directional high-throughput sequencing of RNAs without gene-specific primers.', 'authors' => 'Mäki A, Tiirola M', 'description' => '<p>Ribosomal RNA analysis is a useful tool for characterization of microbial communities. However, the lack of broad-range primers has hampered the simultaneous analysis of eukaryotic and prokaryotic members by amplicon sequencing. We present a complete workflow for directional, primer-independent sequencing of size-selected small subunit ribosomal RNA fragments. The library preparation protocol includes gel extraction of the target RNA, ligation of an RNA oligo to the 5'-end of the target, and cDNA synthesis with a tailed random-hexamer primer and further barcoding. The sequencing results of a phytoplankton mock community showed a highly similar profile to the biomass indicators. This method has universal potential for microbiome studies, and is compatible for the 5'-end sequencing of other RNA types with minimum library preparation costs.</p>', 'date' => '2018-10-05', 'pmid' => 'http://www.pubmed.gov/30284935', 'doi' => '10.2144/btn-2018-0082', 'modified' => '2018-12-31 11:24:16', 'created' => '2018-11-08 12:59:45', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/CATS-RNA-seq-Kit' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $pro = array( 'id' => '2881', 'antibody_id' => null, 'name' => 'CATS RNA-seq Kit v2 x96', 'description' => '<p style="text-align: justify;">Diagenode’s CATS RNA-seq Kit utilizes the innovative “<strong>C</strong>apture and <strong>A</strong>mplification by <strong>T</strong>ailing and <strong>S</strong>witching” (CATS), a <strong>ligation-free method</strong> to produce DNA libraries for next generation sequencing from low input amounts of RNA.</p> <ul> <li>Diverse transcripts detection</li> <li>Excellent reproducibility with ultra low inputs down to 100 pg</li> <li>Great performance on challenging samples</li> </ul> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#more" style="color: #13b29c; background-color: transparent; display: inline; padding: 0;">Read more</a> <div id="more" class="content"> <p style="text-align: justify;">CATS relies on polynucleotide tailing for capturing the 3’-ends of nucleic acids and the template switching ability of MMLV-RT to capture the 5’ ends for cDNA synthesis. This kit allows for generating Illumina compatible ready-to-sequence libraries from RNA inputs as low as <strong>100 picograms</strong> (<strong>rRNA depleted or polyA selected</strong>) in just a few hours.</p> <p style="text-align: justify;">Libraries retain <strong>strand specificity</strong> of origin. Unlike competing solutions, our CATS RNA-seq kit exhibits <strong>higher library efficiency</strong> versus ligation-based methods providing <strong>higher complexity</strong> from better template capture and minimal bias due to <strong>lower amplification</strong> requirements.</p> </div> </li> </ul> <div class="carrousel" style="background-position: center;"> <div class="container"> <div class="row" style="background: rgba(255,255,255,0.1); height: 241px;"> <div class="large-12 columns slick"> <div> <h3>Broad overview of the transcriptome</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/polyA-mRNA-seq-1ng-10ng .png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Biotypes* represented in the libraries <strong>CATS v2 mRNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2. <br />Input: <strong>1 ng, 10 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000). * < 0.04% of total detected transcripts at this threshold is rRNA</p> </div> </div> <div> <h3>More transcripts detected</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/rRNA-total-RNA-seq-1ng-10ng.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Biotypes* represented in the libraries <strong>CATS v2 total RNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2. <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000). * < 2% of total detected transcripts at this threshold is rRNA</p> </div> </div> <div> <h3>Uniquely detected transcripts</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_1_M1_all_CATS_NEB_nolimit_NEW.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>All detected transcripts in <strong>CATS v2 mRNA-seq Kit</strong> vs NEBNext Ultra I directional library preparation Kit. No threshold has been set for the analysis. <br />Input: <strong>1 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000). 36,245 transcripts found in both libraries.</p> </div> </div> <div> <h3>Consistency and wide representation of the transcriptome</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_2_M1_coding_CATS_SM.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Comparison on the number of coding transcripts detected with <strong>CATS v2 mRNA-seq Kit</strong> vs SMARTer stranded RNA-seq library preparation kit. TPM ≥2. <br />Input: <strong>1 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000). 21,333 transcripts found in both libraries.</p> </div> </div> <div> <h3>High-complexity at low inputs</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/high-complexity-low-inputs.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Mapped reads distribution for <strong>CATS v2 mRNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. <br />Input: <strong>1 ng, 10 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>Very reproducible library preparation method</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_4_R1_scatter_replicates_CATS.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Transcripts detected in two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>High correlation in transcripts detection across ultra low inputs</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_6_v1_M_scatter_inputs_CATS.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Transcripts detected in 1 ng vs 0.1 ng samples of <strong>CATS v2 mRNA-seq libraries. </strong>TPM≥2. <br />Input: <strong>1 ng, 0.1 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>High correlation in coding transcripts detection across different inputs</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_7_R_scatter_inputs_CATS.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Transcripts detected in 10 ng vs 1 ng samples of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>Few PCR cycles, less amplification bias</h3> <div class="large-12 small-12 medium-12 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-Fig_1ng-rRNAdplt-RNA-CATSV2.png" /></div> <div class="large-12 small-12 medium-12 columns"> <p>BioAnalyzer® electropherogram of two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>Excellent performance on challenging samples</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/Exosomes-page_heatt-1.jpg" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Heatmap clustering of the top 50 differentially expressed miRNAs after library preparation:</p> <ul> <li><strong>CATS Small RNA-seq Kit</strong> on 1 ng exosomal RNA (Exosome sample 1, 2)</li> <li><strong>CATS RNA-seq Kit</strong> on 1 ng total RNA from parent (HEK293T) cells (Cell sample 1, 2).</li> </ul> <p>Prior library preparation, exosomes were isolated with Diagenode exosome capture solutions <a href="../categories/exosomes">Exosomes</a></p> </div> </div> <div> <h3>CATS saves time</h3> <img src="https://www.diagenode.com/img/categories/library-prep/cats-save-time.png" /></div> </div> </div> </div> </div>', 'label1' => 'Characteristics', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '96 rxns', 'catalog_number' => 'C05010049', 'old_catalog_number' => '', 'sf_code' => 'C05010049-', 'type' => 'RFR', 'search_order' => '', 'price_EUR' => '4774', 'price_USD' => '4774', 'price_GBP' => '4020', 'price_JPY' => '831000', 'price_CNY' => '', 'price_AUD' => '11935', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => false, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'cats-rna-seq-kit-v2-96', 'meta_title' => 'CATS RNA-seq Kit v2 x96 | Diagenode', 'meta_keywords' => 'Capture and Amplification by Tailing and Switching(CATS), RNA-seq, non-coding RNA, long non-coding RNA, whole transcriptome, RNA-seq library preparation,higher RNA diversity, low input, easy, user-friendly', 'meta_description' => 'Capture and Amplification by Tailing and Switching RNA-seq Kit is a ligation-free assay providng increased efficiency and minimum bias, with low inputs down to 100 picograms. User-friendly with reduced hands-on time and fewer steps than competing solution', 'modified' => '2021-05-05 10:47:41', 'created' => '2017-03-07 23:18:46', 'ProductsGroup' => array( 'id' => '232', 'product_id' => '2881', 'group_id' => '207' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/D-Plex-Total-RNA-seq-Library-Prep-x24"><img src="/img/product/kits/dplex/total-dplex-1.jpg" alt="D-Plex Total RNA-seq Kit for Illumina" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C05030031</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3189" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/3189" id="CartAdd/3189Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3189" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> D-Plex Total RNA-seq Kit for Illumina</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('D-Plex Total RNA-seq Kit for Illumina', 'C05030031', '1475', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('D-Plex Total RNA-seq Kit for Illumina', 'C05030031', '1475', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="D-Plex-Total-RNA-seq-Library-Prep-x24" data-reveal-id="cartModal-3189" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">D-Plex Total RNA-seq Kit for Illumina</h6> </div> </div> </li> ' $related = array( 'id' => '3189', 'antibody_id' => null, 'name' => 'D-Plex Total RNA-seq Kit for Illumina', 'description' => '<div class="small-12 medium-12 large-12 columns" style="border: 3px solid #B02736; padding: 10px; margin: 10px;"> <h2>Product Features</h2> <ul style="list-style-type: disc;"> <li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li> <li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li> <li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li> <li><span>Get high sensitivity data even from difficult samples, such as degraded, FFPE samples</span></li> <li><span style="font-weight: 400;">Enjoy a fast, easy, single tube protocol</span></li> </ul> </div> <p></p> <p></p> <h4></h4> <center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center> <p></p> <p>D-Plex Total RNA-seq Library Preparation Kit is a tool designed for the study of the whole coding and non-coding transcriptome. <span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span> </span>D-Plex technology</a><span><span> </span>to generate directional libraries for Illumina sequencing directly from total RNAs, mRNAs that has already been enriched by poly(A) selection, or RNAs that has already been depleted of rRNAs.</span></p> <p><span>The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p> <p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. <span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.</span></span></p> <p><span><span>D-Plex Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p> <ul> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C"><span>C05030023</span> - D-Plex Unique Dual Indexes for Illumina - Set C </a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span> - D-Plex Unique Dual Indexes for Illumina - Set D </a></li> </ul> <div class="extra-spaced" align="center"></div> <div class="row"> <div class="carrousel" style="background-position: center;"> <div class="slick"> <div> <h3>Greater sensitivity to detect novel transcripts</h3> <div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div> <div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"> <p></p> <p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p> </div> </div> <div> <h3>Highest diversity to facilitate biomarker discovery</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p> </div> </div> <div> <h3>Consistent expression profiling across a wide range of RNA inputs</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p> </div> </div> <div> <h3>Superior library complexity at low RNA inputs</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p> </div> </div> </div> </div> </div>', 'label1' => '', 'info1' => '', 'label2' => 'Indexes', 'info2' => '<p><span>Specific D-Plex indexes </span><span>were designed and validated to fit the D-Plex technology for Illumina sequencing and </span><span>are not included in the kit. They can be bought separately according to your needs. Please choose the format that suits you best among the compatible references to:</span></p> <ul> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C" title="D-Plex UDI Module - Set C" target="_blank"><span>C05030023</span> - D-Plex Unique Dual Indexes for Illumina - Set C</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span> - D-Plex Unique Dual Indexes for Illumina - Set D </a></li> </ul> <p></p> <p>The use of UDI is highly recommended to mitigate errors introduced by read misassignment, including index hopping frequently observed with patterned flow cells such as Illumina’s NovaSeq system.</p>', 'label3' => 'Data analysis', 'info3' => '<p><span>A specific bioinformatics pipeline has been developed to process the special sequences present in the D-Plex construct, namely the UMI, the A-tail, and the template switch motif. 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Subject to the compatibility of D-Plex constructs, other specific pipelines can be used.</span></p> <p><img src="https://www.diagenode.com/img/product/kits/dplex/bioinfoPipe.png" alt="Small RNA seq Bioinformatics pipeline" width="925" height="196" /></p>', 'format' => '24 rxns', 'catalog_number' => 'C05030031', 'old_catalog_number' => '', 'sf_code' => 'C05030031-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '1475', 'price_USD' => '1475', 'price_GBP' => '1355', 'price_JPY' => '231060', 'price_CNY' => '', 'price_AUD' => '3688', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'D-Plex-Total-RNA-seq-Library-Prep-x24', 'meta_title' => 'D-Plex Total RNA-seq Library Prep Kit for Illumina | Diagenode ', 'meta_keywords' => 'RNA-seq kit, RNA-seq library preparation; low input RNA-seq, UMI RNA-seq, UDI RNA-seq, D-Plex, higher RNA sensitivity', 'meta_description' => 'RNA-seq library preparation for Illumina sequencing - Unique D-Plex technology - Optimized for ultra-low input (50 pg total RNA) - UMI reduce PCR biases - UDI detect index hopping - Compatibility with FFPE samples - User-friendly and fast protocol', 'modified' => '2023-11-16 09:09:25', 'created' => '2021-04-13 14:10:40', 'ProductsRelated' => array( 'id' => '4753', 'product_id' => '2844', 'related_id' => '3189' ), 'Image' => array( (int) 0 => array( 'id' => '1817', 'name' => 'product/kits/dplex/total-dplex-1.jpg', 'alt' => 'D-Plex Total RNA-seq Kit for Illumina', 'modified' => '2021-05-11 16:42:58', 'created' => '2021-05-11 16:42:58', 'ProductsImage' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1818', 'name' => 'product/kits/dplex/total-dplex-2.jpg', 'alt' => 'D-Plex Total RNA-seq Kit for Illumina', 'modified' => '2021-05-11 16:43:26', 'created' => '2021-05-11 16:43:26', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '1134', 'name' => 'D-Plex Total RNA-seq Kit', 'description' => '<p class="p1">The Diagenode D-Plex Total RNA-seq Library Preparation kit is a tool designed for the study of the whole coding and non-coding transcriptome. The present kit incorporates the unique D-Plex technology to generate directional RNA libraries for Illumina sequencing directly from total RNAs, messenger RNAs (mRNAs) that have already been enriched by poly(A) capture, or RNAs that have already been depleted of ribosomal RNAs (rRNAs).</p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/dplex-total-rnaseq.pdf', 'slug' => 'dplex-total-rnaseq', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-04-20 14:09:33', 'created' => '2021-04-20 14:09:33', 'ProductsDocument' => array( 'id' => '3122', 'product_id' => '2844', 'document_id' => '1134' ) ) $publication = array( 'id' => '3401', 'name' => 'Directional high-throughput sequencing of RNAs without gene-specific primers.', 'authors' => 'Mäki A, Tiirola M', 'description' => '<p>Ribosomal RNA analysis is a useful tool for characterization of microbial communities. 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User-friendly with reduced hands-on time and fewer steps than competing solution', 'modified' => '2021-05-07 15:31:50', 'created' => '2016-10-21 06:18:57' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '3189', 'antibody_id' => null, 'name' => 'D-Plex Total RNA-seq Kit for Illumina', 'description' => '<div class="small-12 medium-12 large-12 columns" style="border: 3px solid #B02736; padding: 10px; margin: 10px;"> <h2>Product Features</h2> <ul style="list-style-type: disc;"> <li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li> <li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li> <li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li> <li><span>Get high sensitivity data even from difficult samples, such as degraded, FFPE samples</span></li> <li><span style="font-weight: 400;">Enjoy a fast, easy, single tube protocol</span></li> </ul> </div> <p></p> <p></p> <h4></h4> <center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center> <p></p> <p>D-Plex Total RNA-seq Library Preparation Kit is a tool designed for the study of the whole coding and non-coding transcriptome. <span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span> </span>D-Plex technology</a><span><span> </span>to generate directional libraries for Illumina sequencing directly from total RNAs, mRNAs that has already been enriched by poly(A) selection, or RNAs that has already been depleted of rRNAs.</span></p> <p><span>The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p> <p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. <span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.</span></span></p> <p><span><span>D-Plex Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p> <ul> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C"><span>C05030023</span> - D-Plex Unique Dual Indexes for Illumina - Set C </a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span> - D-Plex Unique Dual Indexes for Illumina - Set D </a></li> </ul> <div class="extra-spaced" align="center"></div> <div class="row"> <div class="carrousel" style="background-position: center;"> <div class="slick"> <div> <h3>Greater sensitivity to detect novel transcripts</h3> <div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div> <div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"> <p></p> <p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p> </div> </div> <div> <h3>Highest diversity to facilitate biomarker discovery</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p> </div> </div> <div> <h3>Consistent expression profiling across a wide range of RNA inputs</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p> </div> </div> <div> <h3>Superior library complexity at low RNA inputs</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p> </div> </div> </div> </div> </div>', 'label1' => '', 'info1' => '', 'label2' => 'Indexes', 'info2' => '<p><span>Specific D-Plex indexes </span><span>were designed and validated to fit the D-Plex technology for Illumina sequencing and </span><span>are not included in the kit. They can be bought separately according to your needs. Please choose the format that suits you best among the compatible references to:</span></p> <ul> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C" title="D-Plex UDI Module - Set C" target="_blank"><span>C05030023</span> - D-Plex Unique Dual Indexes for Illumina - Set C</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span> - D-Plex Unique Dual Indexes for Illumina - Set D </a></li> </ul> <p></p> <p>The use of UDI is highly recommended to mitigate errors introduced by read misassignment, including index hopping frequently observed with patterned flow cells such as Illumina’s NovaSeq system.</p>', 'label3' => 'Data analysis', 'info3' => '<p><span>A specific bioinformatics pipeline has been developed to process the special sequences present in the D-Plex construct, namely the UMI, the A-tail, and the template switch motif. All guidelines and free softwares are shared in the user manual. Subject to the compatibility of D-Plex constructs, other specific pipelines can be used.</span></p> <p><img src="https://www.diagenode.com/img/product/kits/dplex/bioinfoPipe.png" alt="Small RNA seq Bioinformatics pipeline" width="925" height="196" /></p>', 'format' => '24 rxns', 'catalog_number' => 'C05030031', 'old_catalog_number' => '', 'sf_code' => 'C05030031-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '1475', 'price_USD' => '1475', 'price_GBP' => '1355', 'price_JPY' => '231060', 'price_CNY' => '', 'price_AUD' => '3688', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'D-Plex-Total-RNA-seq-Library-Prep-x24', 'meta_title' => 'D-Plex Total RNA-seq Library Prep Kit for Illumina | Diagenode ', 'meta_keywords' => 'RNA-seq kit, RNA-seq library preparation; low input RNA-seq, UMI RNA-seq, UDI RNA-seq, D-Plex, higher RNA sensitivity', 'meta_description' => 'RNA-seq library preparation for Illumina sequencing - Unique D-Plex technology - Optimized for ultra-low input (50 pg total RNA) - UMI reduce PCR biases - UDI detect index hopping - Compatibility with FFPE samples - User-friendly and fast protocol', 'modified' => '2023-11-16 09:09:25', 'created' => '2021-04-13 14:10:40', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '14', 'position' => '10', 'parent_id' => '3', 'name' => 'DNA/RNA library preparation', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><span style="font-weight: 400;">Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to </span><a href="../applications/dna-rna-shearing"><span style="font-weight: 400;">fragmented DNA or RNA</span></a><span style="font-weight: 400;"> prior to sequencing</span></p> <p><span style="font-weight: 400;">After input DNA has been fragmented, it is end-repaired and blunt-ended</span><span style="font-weight: 400;">. The next step is a A-tailing in which dAMP is added to the 3´ end of the blunt phosphorylated DNA fragments to prevent concatemerization and to allow the ligation of adaptors with complementary dT overhangs. In addition, barcoded adapters can be incorporated to facilitate multiplexing prior to or during amplification.</span></p> <center><img src="https://www.diagenode.com/img/categories/library-prep/flux.png" /></center> <p><span style="font-weight: 400;">Diagenode offers a comprehensive product portfolio for library preparation:<br /></span></p> <strong><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex RNA-seq Library Preparation Kits</a></strong><br /> <p><span style="font-weight: 400;">Diagenode’s new RNA-sequencing solutions utilize the innovative c</span><span style="font-weight: 400;">apture and a</span><span style="font-weight: 400;">mplification by t</span><span style="font-weight: 400;">ailing and s</span><span style="font-weight: 400;">witching”</span><span style="font-weight: 400;">, a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of RNA. </span><span style="font-weight: 400;"></span><a href="../categories/Library-preparation-for-RNA-seq">Learn more</a></p> <strong><a href="../categories/library-preparation-for-ChIP-seq">ChIP-seq and DNA sequencing library preparation solutions</a></strong><br /> <p><span style="font-weight: 400;">Our kits have been optimized for DNA library preparation used for next generation sequencing for a wide range of inputs. Using a simple three-step protocols, our</span><a href="http://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><span style="font-weight: 400;"> </span></a><span style="font-weight: 400;">kits are an optimal choice for library preparation from DNA inputs down to 50 pg. </span><a href="../categories/library-preparation-for-ChIP-seq">Learn more</a></p> <a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span><strong>Bioruptor Pico - short fragments</strong></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br /> <p><span style="font-weight: 400;"></span><span style="font-weight: 400;">Our well-cited Bioruptor Pico is the shearing device of choice for chromatin and DNA fragmentation. Obtain uniform and tight fragment distributions between 150bp -2kb. </span><a href="../p/bioruptor-pico-sonication-device">Learn more</a></p> <strong><a href="../p/megaruptor2-1-unit"><span href="../p/bioruptor-pico-sonication-device">Megaruptor</span>® - long fragments</a></strong><a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br /> <p><span style="font-weight: 400;"></span><span style="font-weight: 400;">The Megaruptor is designed to shear DNA from 3kb-75kb for long-read sequencing. <a href="../p/megaruptor2-1-unit">Learn more</a></span></p> <span href="../p/bioruptor-pico-sonication-device"></span><span style="font-weight: 400;"></span></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'library-preparation', 'meta_keywords' => 'Library preparation,Next Generation Sequencing,DNA fragments,MicroPlex Library,iDeal Library', 'meta_description' => 'Diagenode offers a comprehensive product portfolio for library preparation such as CATS RNA-seq Library Preparation Kits,ChIP-seq and DNA sequencing library preparation solutions', 'meta_title' => 'Library preparation for Next Generation Sequencing (NGS) | Diagenode', 'modified' => '2021-03-10 03:27:16', 'created' => '2014-08-16 07:47:56', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3', 'position' => '10', 'parent_id' => null, 'name' => '次世代シーケンシング', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns"> <h2 style="font-size: 22px;">DNA断片化、ライブラリー調製、自動化：NGSのワンストップショップ</h2> <table class="small-12 medium-12 large-12 columns"> <tbody> <tr> <th class="small-12 medium-12 large-12 columns"> <h4>1. 断片化装置を選択してください：150 bp〜75 kbの範囲でDNAを断片化します。</h4> </th> </tr> <tr style="background-color: #ffffff;"> <td class="small-12 medium-12 large-12 columns"></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-pico-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/bioruptor_pico.jpg" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/megaruptor2-1-unit"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010001_megaruptor2.jpg" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-one-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-profil.png" /></a></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns">5μlまで断片化：150 bp〜2 kb<br />NGS DNAライブラリー調製およびFFPE核酸抽出に最適で、</td> <td class="small-4 medium-4 large-4 columns">2 kb〜75 kbの範囲をできます。<br />メイトペアライブラリー調製および長いフラグメントDNAシーケンシングに最適で、この軽量デスクトップデバイスで</td> <td class="small-4 medium-4 large-4 columns">20または50μlの断片化が可能です。</td> </tr> </tbody> </table> <table class="small-12 medium-12 large-12 columns"> <tbody> <tr> <th class="small-8 medium-8 large-8 columns"> <h4>2. 最適化されたライブラリー調整キットを選択してください。</h4> </th> <th class="small-4 medium-4 large-4 columns"> <h4>3. ライブラリー前処理自動化を選択して、比類のないデータ再現性を実感</h4> </th> </tr> <tr style="background-color: #ffffff;"> <td class="small-12 medium-12 large-12 columns"></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns"><a href="../p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><img src="https://www.diagenode.com/img/product/kits/microPlex_library_preparation.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/ideal-library-preparation-kit-x24-incl-index-primer-set-1-24-rxns"><img src="https://www.diagenode.com/img/product/kits/box_kit.jpg" style="display: block; margin-left: auto; margin-right: auto;" height="173" width="250" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/sx-8g-ip-star-compact-automated-system-1-unit"><img src="https://www.diagenode.com/img/product/automation/B03000002%20_ipstar_compact.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">50pgの低入力：MicroPlex Library Preparation Kit</td> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">5ng以上：iDeal Library Preparation Kit</td> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">Achieve great NGS data easily</td> </tr> </tbody> </table> </div> </div> <blockquote> <div class="row"> <div class="small-12 medium-12 large-12 columns"><span class="label" style="margin-bottom: 16px; margin-left: -22px; font-size: 15px;">DiagenodeがNGS研究にぴったりなプロバイダーである理由</span> <p>Diagenodeは15年以上もエピジェネティクス研究に専念、専門としています。 ChIP研究クロマチン用のユニークな断片化システムの開発から始まり、 専門知識を活かし、5μlのせん断体積まで可能で、NGS DNAライブラリーの調製に最適な最先端DNA断片化装置の開発にたどり着きました。 我々は以来、ChIP-seq、Methyl-seq、NGSライブラリー調製用キットを研究開発し、業界をリードする免疫沈降研究と同様に、ライブラリー調製を自動化および完結させる独自の自動化システムを開発にも成功しました。</p> <ul> <li>信頼されるせん断装置</li> <li>様々なインプットからのライブラリ作成キット</li> <li>独自の自動化デバイス</li> </ul> </div> </div> </blockquote> <div class="row"> <div class="small-12 columns"> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#panel1a">次世代シーケンシングへの理解とその専門知識</a> <div id="panel1a" class="content"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong>次世代シーケンシング (NGS)</strong> ）は、著しいスケールとハイスループットでシーケンシングを行い、1日に数十億もの塩基生成を可能にします。 NGSのハイスループットは迅速でありながら正確で、再現性のあるデータセットを実現し、さらにシーケンシング費用を削減します。 NGSは、ゲノムシーケンシング、ゲノム再シーケンシング、デノボシーケンシング、トランスクリプトームシーケンシング、その他にDNA-タンパク質相互作用の検出やエピゲノムなどを示します。 指数関数的に増加するシーケンシングデータの需要は、計算分析の障害や解釈、データストレージなどの課題を解決します。</p> <p>アプリケーションおよび出発物質に応じて、数百万から数十億の鋳型DNA分子を大規模に並行してシーケンシングすることが可能です。その為に、異なる化学物質を使用するいくつかの市販のNGSプラットフォームを利用することができます。 NGSプラットフォームの種類によっては、事前準備とライブラリー作成が必要です。</p> <p>NGSにとっても、特にデータ処理と分析に関した大きな課題はあります。第3世代技術はゲノミクス研究にさらに革命を起こすであろうと大きく期待されています。</p> </div> </div> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <p><strong>NGS アプリケーション</strong></p> <ul> <li>全ゲノム配列決定</li> <li>デノボシーケンシング</li> <li>標的配列</li> <li>Exomeシーケンシング</li> <li>トランスクリプトーム配列決定</li> <li>ゲノム配列決定</li> <li>ミトコンドリア配列決定</li> <li>DNA-タンパク質相互作用（ChIP-seq</li> <li>バリアント検出</li> <li>ゲノム仕上げ</li> </ul> </div> <div class="small-6 medium-6 large-6 columns"> <p><strong>研究分野におけるNGS:</strong></p> <ul> <li>腫瘍学</li> <li>リプロダクティブ・ヘルス</li> <li>法医学ゲノミクス</li> <li>アグリゲノミックス</li> <li>複雑な病気</li> <li>微生物ゲノミクス</li> <li>食品・環境ゲノミクス</li> <li>創薬ゲノミクス - パーソナライズド・メディカル</li> </ul> </div> <div class="small-12 medium-12 large-12 columns"> <p><strong>NGSの用語</strong></p> <dl> <dt>リード（読み取り）</dt> <dd>この装置から得られた連続した単一のストレッチ</dd> <dt>断片リード</dt> <dd>フラグメントライブラリからの読み込み。 シーケンシングプラットフォームに応じて、読み取りは通常約100〜300bp。</dd> <dt>断片ペアエンドリード</dt> <dd>断片ライブラリーからDNA断片の各末端2つの読み取り。</dd> <dt>メイトペアリード</dt> <dd>大きなDNA断片（通常は予め定義されたサイズ範囲）の各末端から2つの読み取り。</dd> <dt>カバレッジ（例）</dt> <dd>30×適用範囲とは、参照ゲノム中の各塩基対が平均30回の読み取りを示す。</dd> </dl> </div> </div> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h2>NGSプラットフォーム</h2> <h3><a href="http://www.illumina.com" target="_blank">イルミナ</a></h3> <p>イルミナは、クローン的に増幅された鋳型DNA（クラスター）上に位置する、蛍光標識された可逆的鎖ターミネーターヌクレオチドを用いた配列別合成技術を使用。 DNAクラスターは、ガラスフローセルの表面上に固定化され、 ワークフローは、4つのヌクレオチド（それぞれ異なる蛍光色素で標識された）の組み込み、4色イメージング、色素や末端基の切断、取り込み、イメージングなどを繰り返します。フローセルは大規模な並列配列決定を受ける。 この方法により、単一蛍光標識されたヌクレオチドの制御添加によるモノヌクレオチドのエラーを回避する可能性があります。 読み取りの長さは、通常約100〜150 bpです。</p> <h3><a href="http://www.lifetechnologies.com" target="_blank">イオン トレント</a></h3> <p>イオントレントは、半導体技術チップを用いて、合成中にヌクレオチドを取り込む際に放出されたプロトンを検出します。 これは、イオン球粒子と呼ばれるビーズの表面にエマルションPCR（emPCR）を使用し、リンクされた特定のアダプターを用いてDNA断片を増幅します。 各ビーズは1種類のDNA断片で覆われていて、異なるDNA断片を有するビーズは次いで、チップの陽子感知ウェル内に配置されます。 チップには一度に4つのヌクレオチドのうちの1つが浸水し、このプロセスは異なるヌクレオチドで15秒ごとに繰り返されます。 配列決定の間に4つの塩基の各々が1つずつ導入されます、組み込みの場合はプロトンが放出され、電圧信号が取り込みに比例して検出されます。.</p> <h3><a href="http://www.pacificbiosciences.com" target="_blank">パシフィック バイオサイエンス</a></h3> <p>パシフィックバイオサイエンスでは、20kbを超える塩基対の読み取りも、単一分子リアルタイム（SMRT）シーケンシングによる構造および細胞タイプの変化を観察することができます。 このプラットフォームでは、超長鎖二本鎖DNA（dsDNA）断片が、Megaruptor（登録商標）のようなDiagenode装置を用いたランダムシアリングまたは目的の標的領域の増幅によって生成されます。 SMRTbellライブラリーは、ユニバーサルヘアピンアダプターをDNA断片の各末端に連結することによって生成します。 サイズ選択条件による洗浄ステップの後、配列決定プライマーをSMRTbellテンプレートにアニーリングし、鋳型DNAに結合したDNAポリメラーゼを含む配列決定を、蛍光標識ヌクレオチドの存在下で開始。 各塩基が取り込まれると、異なる蛍光のパルスをリアルタイムで検出します。</p> <h3><a href="https://nanoporetech.com" target="_blank">オックスフォード ナノポア</a></h3> <p>Oxford Nanoporeは、単一のDNA分子配列決定に基づく技術を開発します。その技術により生物学的分子、すなわちDNAが一群の電気抵抗性高分子膜として位置するナノスケールの孔（ナノ細孔）またはその近くを通過し、イオン電流が変化します。 この変化に関する情報は、例えば4つのヌクレオチド（AまたはG r CまたはT）ならびに修飾されたヌクレオチドすべてを区別することによって分子情報に訳されます。 シーケンシングミニオンデバイスのフローセルは、数百個のナノポアチャネルのセンサアレイを含みます。 DNAサンプルは、Diagenode社のMegaruptor（登録商標）を用いてランダムシアリングによって生成され得る超長鎖DNAフラグメントが必要です。</p> <h3><a href="http://www.lifetechnologies.com/be/en/home/life-science/sequencing/next-generation-sequencing/solid-next-generation-sequencing.html" target="_blank">SOLiD</a></h3> <p>SOLiDは、ユニークな化学作用により、何千という個々のDNA分子の同時配列決定を可能にします。 それは、アダプター対ライブラリーのフラグメントが適切で、せん断されたゲノムDNAへのアダプターのライゲーションによるライブラリー作製から始まります。 次のステップでは、エマルジョンPCR（emPCR）を実施して、ビーズの表面上の個々の鋳型DNA分子をクローン的に増幅。 emPCRでは、個々の鋳型DNAをPCR試薬と混合し、水中油型エマルジョン内の疎水性シェルで囲まれた水性液滴内のプライマーコートビーズを、配列決定のためにロードするスライドガラスの表面にランダムに付着。 この技術は、シークエンシングプライマーへのライゲーションで競合する4つの蛍光標識されたジ塩基プローブのセットを使用します。</p> <h3><a href="http://454.com/products/technology.asp" target="_blank">454</a></h3> <p>454は、大規模並列パイロシーケンシングを利用しています。 始めに全ゲノムDNAまたは標的遺伝子断片の300〜800bp断片のライブラリー調製します。 次に、DNAフラグメントへのアダプターの付着および単一のDNA鎖の分離。 その後アダプターに連結されたDNAフラグメントをエマルジョンベースのクローン増幅（emPCR）で処理し、DNAライブラリーフラグメントをミクロンサイズのビーズ上に配置します。 各DNA結合ビーズを光ファイバーチップ上のウェルに入れ、器具に挿入します。 4つのDNAヌクレオチドは、配列決定操作中に固定された順序で連続して加えられ、並行して配列決定されます。</p> </div> </div> </div> </li> </ul> </div> </div>', 'in_footer' => true, 'in_menu' => true, 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The present kit incorporates the unique D-Plex technology to generate directional RNA libraries for Illumina sequencing directly from total RNAs, messenger RNAs (mRNAs) that have already been enriched by poly(A) capture, or RNAs that have already been depleted of ribosomal RNAs (rRNAs).</p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/dplex-total-rnaseq.pdf', 'slug' => 'dplex-total-rnaseq', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-04-20 14:09:33', 'created' => '2021-04-20 14:09:33', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1777', 'name' => 'product/kits/library-prep-kit-icon.png', 'alt' => 'Library prep kit icon', 'modified' => '2018-03-15 15:51:51', 'created' => '2018-03-15 15:51:26', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3401', 'name' => 'Directional high-throughput sequencing of RNAs without gene-specific primers.', 'authors' => 'Mäki A, Tiirola M', 'description' => '<p>Ribosomal RNA analysis is a useful tool for characterization of microbial communities. However, the lack of broad-range primers has hampered the simultaneous analysis of eukaryotic and prokaryotic members by amplicon sequencing. We present a complete workflow for directional, primer-independent sequencing of size-selected small subunit ribosomal RNA fragments. The library preparation protocol includes gel extraction of the target RNA, ligation of an RNA oligo to the 5'-end of the target, and cDNA synthesis with a tailed random-hexamer primer and further barcoding. The sequencing results of a phytoplankton mock community showed a highly similar profile to the biomass indicators. This method has universal potential for microbiome studies, and is compatible for the 5'-end sequencing of other RNA types with minimum library preparation costs.</p>', 'date' => '2018-10-05', 'pmid' => 'http://www.pubmed.gov/30284935', 'doi' => '10.2144/btn-2018-0082', 'modified' => '2018-12-31 11:24:16', 'created' => '2018-11-08 12:59:45', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/CATS-RNA-seq-Kit' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $pro = array( 'id' => '2881', 'antibody_id' => null, 'name' => 'CATS RNA-seq Kit v2 x96', 'description' => '<p style="text-align: justify;">Diagenode’s CATS RNA-seq Kit utilizes the innovative “<strong>C</strong>apture and <strong>A</strong>mplification by <strong>T</strong>ailing and <strong>S</strong>witching” (CATS), a <strong>ligation-free method</strong> to produce DNA libraries for next generation sequencing from low input amounts of RNA.</p> <ul> <li>Diverse transcripts detection</li> <li>Excellent reproducibility with ultra low inputs down to 100 pg</li> <li>Great performance on challenging samples</li> </ul> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#more" style="color: #13b29c; background-color: transparent; display: inline; padding: 0;">Read more</a> <div id="more" class="content"> <p style="text-align: justify;">CATS relies on polynucleotide tailing for capturing the 3’-ends of nucleic acids and the template switching ability of MMLV-RT to capture the 5’ ends for cDNA synthesis. This kit allows for generating Illumina compatible ready-to-sequence libraries from RNA inputs as low as <strong>100 picograms</strong> (<strong>rRNA depleted or polyA selected</strong>) in just a few hours.</p> <p style="text-align: justify;">Libraries retain <strong>strand specificity</strong> of origin. Unlike competing solutions, our CATS RNA-seq kit exhibits <strong>higher library efficiency</strong> versus ligation-based methods providing <strong>higher complexity</strong> from better template capture and minimal bias due to <strong>lower amplification</strong> requirements.</p> </div> </li> </ul> <div class="carrousel" style="background-position: center;"> <div class="container"> <div class="row" style="background: rgba(255,255,255,0.1); height: 241px;"> <div class="large-12 columns slick"> <div> <h3>Broad overview of the transcriptome</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/polyA-mRNA-seq-1ng-10ng .png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Biotypes* represented in the libraries <strong>CATS v2 mRNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2. <br />Input: <strong>1 ng, 10 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000). * < 0.04% of total detected transcripts at this threshold is rRNA</p> </div> </div> <div> <h3>More transcripts detected</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/rRNA-total-RNA-seq-1ng-10ng.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Biotypes* represented in the libraries <strong>CATS v2 total RNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2. <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000). * < 2% of total detected transcripts at this threshold is rRNA</p> </div> </div> <div> <h3>Uniquely detected transcripts</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_1_M1_all_CATS_NEB_nolimit_NEW.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>All detected transcripts in <strong>CATS v2 mRNA-seq Kit</strong> vs NEBNext Ultra I directional library preparation Kit. No threshold has been set for the analysis. <br />Input: <strong>1 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000). 36,245 transcripts found in both libraries.</p> </div> </div> <div> <h3>Consistency and wide representation of the transcriptome</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_2_M1_coding_CATS_SM.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Comparison on the number of coding transcripts detected with <strong>CATS v2 mRNA-seq Kit</strong> vs SMARTer stranded RNA-seq library preparation kit. TPM ≥2. <br />Input: <strong>1 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000). 21,333 transcripts found in both libraries.</p> </div> </div> <div> <h3>High-complexity at low inputs</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/high-complexity-low-inputs.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Mapped reads distribution for <strong>CATS v2 mRNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. <br />Input: <strong>1 ng, 10 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>Very reproducible library preparation method</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_4_R1_scatter_replicates_CATS.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Transcripts detected in two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>High correlation in transcripts detection across ultra low inputs</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_6_v1_M_scatter_inputs_CATS.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Transcripts detected in 1 ng vs 0.1 ng samples of <strong>CATS v2 mRNA-seq libraries. </strong>TPM≥2. <br />Input: <strong>1 ng, 0.1 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>High correlation in coding transcripts detection across different inputs</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_7_R_scatter_inputs_CATS.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Transcripts detected in 10 ng vs 1 ng samples of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>Few PCR cycles, less amplification bias</h3> <div class="large-12 small-12 medium-12 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-Fig_1ng-rRNAdplt-RNA-CATSV2.png" /></div> <div class="large-12 small-12 medium-12 columns"> <p>BioAnalyzer® electropherogram of two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>Excellent performance on challenging samples</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/Exosomes-page_heatt-1.jpg" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Heatmap clustering of the top 50 differentially expressed miRNAs after library preparation:</p> <ul> <li><strong>CATS Small RNA-seq Kit</strong> on 1 ng exosomal RNA (Exosome sample 1, 2)</li> <li><strong>CATS RNA-seq Kit</strong> on 1 ng total RNA from parent (HEK293T) cells (Cell sample 1, 2).</li> </ul> <p>Prior library preparation, exosomes were isolated with Diagenode exosome capture solutions <a href="../categories/exosomes">Exosomes</a></p> </div> </div> <div> <h3>CATS saves time</h3> <img src="https://www.diagenode.com/img/categories/library-prep/cats-save-time.png" /></div> </div> </div> </div> </div>', 'label1' => 'Characteristics', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '96 rxns', 'catalog_number' => 'C05010049', 'old_catalog_number' => '', 'sf_code' => 'C05010049-', 'type' => 'RFR', 'search_order' => '', 'price_EUR' => '4774', 'price_USD' => '4774', 'price_GBP' => '4020', 'price_JPY' => '831000', 'price_CNY' => '', 'price_AUD' => '11935', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => false, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'cats-rna-seq-kit-v2-96', 'meta_title' => 'CATS RNA-seq Kit v2 x96 | Diagenode', 'meta_keywords' => 'Capture and Amplification by Tailing and Switching(CATS), RNA-seq, non-coding RNA, long non-coding RNA, whole transcriptome, RNA-seq library preparation,higher RNA diversity, low input, easy, user-friendly', 'meta_description' => 'Capture and Amplification by Tailing and Switching RNA-seq Kit is a ligation-free assay providng increased efficiency and minimum bias, with low inputs down to 100 picograms. User-friendly with reduced hands-on time and fewer steps than competing solution', 'modified' => '2021-05-05 10:47:41', 'created' => '2017-03-07 23:18:46', 'ProductsGroup' => array( 'id' => '232', 'product_id' => '2881', 'group_id' => '207' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/D-Plex-Total-RNA-seq-Library-Prep-x24"><img src="/img/product/kits/dplex/total-dplex-1.jpg" alt="D-Plex Total RNA-seq Kit for Illumina" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C05030031</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3189" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/3189" id="CartAdd/3189Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3189" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> D-Plex Total RNA-seq Kit for Illumina</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('D-Plex Total RNA-seq Kit for Illumina', 'C05030031', '1475', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('D-Plex Total RNA-seq Kit for Illumina', 'C05030031', '1475', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="D-Plex-Total-RNA-seq-Library-Prep-x24" data-reveal-id="cartModal-3189" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">D-Plex Total RNA-seq Kit for Illumina</h6> </div> </div> </li> ' $related = array( 'id' => '3189', 'antibody_id' => null, 'name' => 'D-Plex Total RNA-seq Kit for Illumina', 'description' => '<div class="small-12 medium-12 large-12 columns" style="border: 3px solid #B02736; padding: 10px; margin: 10px;"> <h2>Product Features</h2> <ul style="list-style-type: disc;"> <li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li> <li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li> <li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li> <li><span>Get high sensitivity data even from difficult samples, such as degraded, FFPE samples</span></li> <li><span style="font-weight: 400;">Enjoy a fast, easy, single tube protocol</span></li> </ul> </div> <p></p> <p></p> <h4></h4> <center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center> <p></p> <p>D-Plex Total RNA-seq Library Preparation Kit is a tool designed for the study of the whole coding and non-coding transcriptome. <span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span> </span>D-Plex technology</a><span><span> </span>to generate directional libraries for Illumina sequencing directly from total RNAs, mRNAs that has already been enriched by poly(A) selection, or RNAs that has already been depleted of rRNAs.</span></p> <p><span>The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p> <p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. <span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.</span></span></p> <p><span><span>D-Plex Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p> <ul> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C"><span>C05030023</span> - D-Plex Unique Dual Indexes for Illumina - Set C </a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span> - D-Plex Unique Dual Indexes for Illumina - Set D </a></li> </ul> <div class="extra-spaced" align="center"></div> <div class="row"> <div class="carrousel" style="background-position: center;"> <div class="slick"> <div> <h3>Greater sensitivity to detect novel transcripts</h3> <div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div> <div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"> <p></p> <p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p> </div> </div> <div> <h3>Highest diversity to facilitate biomarker discovery</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p> </div> </div> <div> <h3>Consistent expression profiling across a wide range of RNA inputs</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p> </div> </div> <div> <h3>Superior library complexity at low RNA inputs</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p> </div> </div> </div> </div> </div>', 'label1' => '', 'info1' => '', 'label2' => 'Indexes', 'info2' => '<p><span>Specific D-Plex indexes </span><span>were designed and validated to fit the D-Plex technology for Illumina sequencing and </span><span>are not included in the kit. They can be bought separately according to your needs. Please choose the format that suits you best among the compatible references to:</span></p> <ul> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C" title="D-Plex UDI Module - Set C" target="_blank"><span>C05030023</span> - D-Plex Unique Dual Indexes for Illumina - Set C</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span> - D-Plex Unique Dual Indexes for Illumina - Set D </a></li> </ul> <p></p> <p>The use of UDI is highly recommended to mitigate errors introduced by read misassignment, including index hopping frequently observed with patterned flow cells such as Illumina’s NovaSeq system.</p>', 'label3' => 'Data analysis', 'info3' => '<p><span>A specific bioinformatics pipeline has been developed to process the special sequences present in the D-Plex construct, namely the UMI, the A-tail, and the template switch motif. 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The present kit incorporates the unique D-Plex technology to generate directional RNA libraries for Illumina sequencing directly from total RNAs, messenger RNAs (mRNAs) that have already been enriched by poly(A) capture, or RNAs that have already been depleted of ribosomal RNAs (rRNAs).</p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/dplex-total-rnaseq.pdf', 'slug' => 'dplex-total-rnaseq', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-04-20 14:09:33', 'created' => '2021-04-20 14:09:33', 'ProductsDocument' => array( 'id' => '3122', 'product_id' => '2844', 'document_id' => '1134' ) ) $publication = array( 'id' => '3401', 'name' => 'Directional high-throughput sequencing of RNAs without gene-specific primers.', 'authors' => 'Mäki A, Tiirola M', 'description' => '<p>Ribosomal RNA analysis is a useful tool for characterization of microbial communities. 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User-friendly with reduced hands-on time and fewer steps than competing solution', 'modified' => '2021-05-07 15:31:50', 'created' => '2016-10-21 06:18:57' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '3189', 'antibody_id' => null, 'name' => 'D-Plex Total RNA-seq Kit for Illumina', 'description' => '<div class="small-12 medium-12 large-12 columns" style="border: 3px solid #B02736; padding: 10px; margin: 10px;"> <h2>Product Features</h2> <ul style="list-style-type: disc;"> <li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li> <li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li> <li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li> <li><span>Get high sensitivity data even from difficult samples, such as degraded, FFPE samples</span></li> <li><span style="font-weight: 400;">Enjoy a fast, easy, single tube protocol</span></li> </ul> </div> <p></p> <p></p> <h4></h4> <center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center> <p></p> <p>D-Plex Total RNA-seq Library Preparation Kit is a tool designed for the study of the whole coding and non-coding transcriptome. <span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span> </span>D-Plex technology</a><span><span> </span>to generate directional libraries for Illumina sequencing directly from total RNAs, mRNAs that has already been enriched by poly(A) selection, or RNAs that has already been depleted of rRNAs.</span></p> <p><span>The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p> <p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. <span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.</span></span></p> <p><span><span>D-Plex Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p> <ul> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C"><span>C05030023</span> - D-Plex Unique Dual Indexes for Illumina - Set C </a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span> - D-Plex Unique Dual Indexes for Illumina - Set D </a></li> </ul> <div class="extra-spaced" align="center"></div> <div class="row"> <div class="carrousel" style="background-position: center;"> <div class="slick"> <div> <h3>Greater sensitivity to detect novel transcripts</h3> <div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div> <div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"> <p></p> <p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p> </div> </div> <div> <h3>Highest diversity to facilitate biomarker discovery</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p> </div> </div> <div> <h3>Consistent expression profiling across a wide range of RNA inputs</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p> </div> </div> <div> <h3>Superior library complexity at low RNA inputs</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p> </div> </div> </div> </div> </div>', 'label1' => '', 'info1' => '', 'label2' => 'Indexes', 'info2' => '<p><span>Specific D-Plex indexes </span><span>were designed and validated to fit the D-Plex technology for Illumina sequencing and </span><span>are not included in the kit. They can be bought separately according to your needs. Please choose the format that suits you best among the compatible references to:</span></p> <ul> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C" title="D-Plex UDI Module - Set C" target="_blank"><span>C05030023</span> - D-Plex Unique Dual Indexes for Illumina - Set C</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span> - D-Plex Unique Dual Indexes for Illumina - Set D </a></li> </ul> <p></p> <p>The use of UDI is highly recommended to mitigate errors introduced by read misassignment, including index hopping frequently observed with patterned flow cells such as Illumina’s NovaSeq system.</p>', 'label3' => 'Data analysis', 'info3' => '<p><span>A specific bioinformatics pipeline has been developed to process the special sequences present in the D-Plex construct, namely the UMI, the A-tail, and the template switch motif. All guidelines and free softwares are shared in the user manual. Subject to the compatibility of D-Plex constructs, other specific pipelines can be used.</span></p> <p><img src="https://www.diagenode.com/img/product/kits/dplex/bioinfoPipe.png" alt="Small RNA seq Bioinformatics pipeline" width="925" height="196" /></p>', 'format' => '24 rxns', 'catalog_number' => 'C05030031', 'old_catalog_number' => '', 'sf_code' => 'C05030031-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '1475', 'price_USD' => '1475', 'price_GBP' => '1355', 'price_JPY' => '231060', 'price_CNY' => '', 'price_AUD' => '3688', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'D-Plex-Total-RNA-seq-Library-Prep-x24', 'meta_title' => 'D-Plex Total RNA-seq Library Prep Kit for Illumina | Diagenode ', 'meta_keywords' => 'RNA-seq kit, RNA-seq library preparation; low input RNA-seq, UMI RNA-seq, UDI RNA-seq, D-Plex, higher RNA sensitivity', 'meta_description' => 'RNA-seq library preparation for Illumina sequencing - Unique D-Plex technology - Optimized for ultra-low input (50 pg total RNA) - UMI reduce PCR biases - UDI detect index hopping - Compatibility with FFPE samples - User-friendly and fast protocol', 'modified' => '2023-11-16 09:09:25', 'created' => '2021-04-13 14:10:40', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '14', 'position' => '10', 'parent_id' => '3', 'name' => 'DNA/RNA library preparation', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><span style="font-weight: 400;">Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to </span><a href="../applications/dna-rna-shearing"><span style="font-weight: 400;">fragmented DNA or RNA</span></a><span style="font-weight: 400;"> prior to sequencing</span></p> <p><span style="font-weight: 400;">After input DNA has been fragmented, it is end-repaired and blunt-ended</span><span style="font-weight: 400;">. The next step is a A-tailing in which dAMP is added to the 3´ end of the blunt phosphorylated DNA fragments to prevent concatemerization and to allow the ligation of adaptors with complementary dT overhangs. In addition, barcoded adapters can be incorporated to facilitate multiplexing prior to or during amplification.</span></p> <center><img src="https://www.diagenode.com/img/categories/library-prep/flux.png" /></center> <p><span style="font-weight: 400;">Diagenode offers a comprehensive product portfolio for library preparation:<br /></span></p> <strong><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex RNA-seq Library Preparation Kits</a></strong><br /> <p><span style="font-weight: 400;">Diagenode’s new RNA-sequencing solutions utilize the innovative c</span><span style="font-weight: 400;">apture and a</span><span style="font-weight: 400;">mplification by t</span><span style="font-weight: 400;">ailing and s</span><span style="font-weight: 400;">witching”</span><span style="font-weight: 400;">, a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of RNA. </span><span style="font-weight: 400;"></span><a href="../categories/Library-preparation-for-RNA-seq">Learn more</a></p> <strong><a href="../categories/library-preparation-for-ChIP-seq">ChIP-seq and DNA sequencing library preparation solutions</a></strong><br /> <p><span style="font-weight: 400;">Our kits have been optimized for DNA library preparation used for next generation sequencing for a wide range of inputs. Using a simple three-step protocols, our</span><a href="http://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><span style="font-weight: 400;"> </span></a><span style="font-weight: 400;">kits are an optimal choice for library preparation from DNA inputs down to 50 pg. </span><a href="../categories/library-preparation-for-ChIP-seq">Learn more</a></p> <a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span><strong>Bioruptor Pico - short fragments</strong></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br /> <p><span style="font-weight: 400;"></span><span style="font-weight: 400;">Our well-cited Bioruptor Pico is the shearing device of choice for chromatin and DNA fragmentation. Obtain uniform and tight fragment distributions between 150bp -2kb. </span><a href="../p/bioruptor-pico-sonication-device">Learn more</a></p> <strong><a href="../p/megaruptor2-1-unit"><span href="../p/bioruptor-pico-sonication-device">Megaruptor</span>® - long fragments</a></strong><a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br /> <p><span style="font-weight: 400;"></span><span style="font-weight: 400;">The Megaruptor is designed to shear DNA from 3kb-75kb for long-read sequencing. <a href="../p/megaruptor2-1-unit">Learn more</a></span></p> <span href="../p/bioruptor-pico-sonication-device"></span><span style="font-weight: 400;"></span></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'library-preparation', 'meta_keywords' => 'Library preparation,Next Generation Sequencing,DNA fragments,MicroPlex Library,iDeal Library', 'meta_description' => 'Diagenode offers a comprehensive product portfolio for library preparation such as CATS RNA-seq Library Preparation Kits,ChIP-seq and DNA sequencing library preparation solutions', 'meta_title' => 'Library preparation for Next Generation Sequencing (NGS) | Diagenode', 'modified' => '2021-03-10 03:27:16', 'created' => '2014-08-16 07:47:56', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3', 'position' => '10', 'parent_id' => null, 'name' => '次世代シーケンシング', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns"> <h2 style="font-size: 22px;">DNA断片化、ライブラリー調製、自動化：NGSのワンストップショップ</h2> <table class="small-12 medium-12 large-12 columns"> <tbody> <tr> <th class="small-12 medium-12 large-12 columns"> <h4>1. 断片化装置を選択してください：150 bp〜75 kbの範囲でDNAを断片化します。</h4> </th> </tr> <tr style="background-color: #ffffff;"> <td class="small-12 medium-12 large-12 columns"></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-pico-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/bioruptor_pico.jpg" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/megaruptor2-1-unit"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010001_megaruptor2.jpg" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-one-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-profil.png" /></a></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns">5μlまで断片化：150 bp〜2 kb<br />NGS DNAライブラリー調製およびFFPE核酸抽出に最適で、</td> <td class="small-4 medium-4 large-4 columns">2 kb〜75 kbの範囲をできます。<br />メイトペアライブラリー調製および長いフラグメントDNAシーケンシングに最適で、この軽量デスクトップデバイスで</td> <td class="small-4 medium-4 large-4 columns">20または50μlの断片化が可能です。</td> </tr> </tbody> </table> <table class="small-12 medium-12 large-12 columns"> <tbody> <tr> <th class="small-8 medium-8 large-8 columns"> <h4>2. 最適化されたライブラリー調整キットを選択してください。</h4> </th> <th class="small-4 medium-4 large-4 columns"> <h4>3. ライブラリー前処理自動化を選択して、比類のないデータ再現性を実感</h4> </th> </tr> <tr style="background-color: #ffffff;"> <td class="small-12 medium-12 large-12 columns"></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns"><a href="../p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><img src="https://www.diagenode.com/img/product/kits/microPlex_library_preparation.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/ideal-library-preparation-kit-x24-incl-index-primer-set-1-24-rxns"><img src="https://www.diagenode.com/img/product/kits/box_kit.jpg" style="display: block; margin-left: auto; margin-right: auto;" height="173" width="250" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/sx-8g-ip-star-compact-automated-system-1-unit"><img src="https://www.diagenode.com/img/product/automation/B03000002%20_ipstar_compact.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">50pgの低入力：MicroPlex Library Preparation Kit</td> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">5ng以上：iDeal Library Preparation Kit</td> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">Achieve great NGS data easily</td> </tr> </tbody> </table> </div> </div> <blockquote> <div class="row"> <div class="small-12 medium-12 large-12 columns"><span class="label" style="margin-bottom: 16px; margin-left: -22px; font-size: 15px;">DiagenodeがNGS研究にぴったりなプロバイダーである理由</span> <p>Diagenodeは15年以上もエピジェネティクス研究に専念、専門としています。 ChIP研究クロマチン用のユニークな断片化システムの開発から始まり、 専門知識を活かし、5μlのせん断体積まで可能で、NGS DNAライブラリーの調製に最適な最先端DNA断片化装置の開発にたどり着きました。 我々は以来、ChIP-seq、Methyl-seq、NGSライブラリー調製用キットを研究開発し、業界をリードする免疫沈降研究と同様に、ライブラリー調製を自動化および完結させる独自の自動化システムを開発にも成功しました。</p> <ul> <li>信頼されるせん断装置</li> <li>様々なインプットからのライブラリ作成キット</li> <li>独自の自動化デバイス</li> </ul> </div> </div> </blockquote> <div class="row"> <div class="small-12 columns"> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#panel1a">次世代シーケンシングへの理解とその専門知識</a> <div id="panel1a" class="content"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong>次世代シーケンシング (NGS)</strong> ）は、著しいスケールとハイスループットでシーケンシングを行い、1日に数十億もの塩基生成を可能にします。 NGSのハイスループットは迅速でありながら正確で、再現性のあるデータセットを実現し、さらにシーケンシング費用を削減します。 NGSは、ゲノムシーケンシング、ゲノム再シーケンシング、デノボシーケンシング、トランスクリプトームシーケンシング、その他にDNA-タンパク質相互作用の検出やエピゲノムなどを示します。 指数関数的に増加するシーケンシングデータの需要は、計算分析の障害や解釈、データストレージなどの課題を解決します。</p> <p>アプリケーションおよび出発物質に応じて、数百万から数十億の鋳型DNA分子を大規模に並行してシーケンシングすることが可能です。その為に、異なる化学物質を使用するいくつかの市販のNGSプラットフォームを利用することができます。 NGSプラットフォームの種類によっては、事前準備とライブラリー作成が必要です。</p> <p>NGSにとっても、特にデータ処理と分析に関した大きな課題はあります。第3世代技術はゲノミクス研究にさらに革命を起こすであろうと大きく期待されています。</p> </div> </div> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <p><strong>NGS アプリケーション</strong></p> <ul> <li>全ゲノム配列決定</li> <li>デノボシーケンシング</li> <li>標的配列</li> <li>Exomeシーケンシング</li> <li>トランスクリプトーム配列決定</li> <li>ゲノム配列決定</li> <li>ミトコンドリア配列決定</li> <li>DNA-タンパク質相互作用（ChIP-seq</li> <li>バリアント検出</li> <li>ゲノム仕上げ</li> </ul> </div> <div class="small-6 medium-6 large-6 columns"> <p><strong>研究分野におけるNGS:</strong></p> <ul> <li>腫瘍学</li> <li>リプロダクティブ・ヘルス</li> <li>法医学ゲノミクス</li> <li>アグリゲノミックス</li> <li>複雑な病気</li> <li>微生物ゲノミクス</li> <li>食品・環境ゲノミクス</li> <li>創薬ゲノミクス - パーソナライズド・メディカル</li> </ul> </div> <div class="small-12 medium-12 large-12 columns"> <p><strong>NGSの用語</strong></p> <dl> <dt>リード（読み取り）</dt> <dd>この装置から得られた連続した単一のストレッチ</dd> <dt>断片リード</dt> <dd>フラグメントライブラリからの読み込み。 シーケンシングプラットフォームに応じて、読み取りは通常約100〜300bp。</dd> <dt>断片ペアエンドリード</dt> <dd>断片ライブラリーからDNA断片の各末端2つの読み取り。</dd> <dt>メイトペアリード</dt> <dd>大きなDNA断片（通常は予め定義されたサイズ範囲）の各末端から2つの読み取り。</dd> <dt>カバレッジ（例）</dt> <dd>30×適用範囲とは、参照ゲノム中の各塩基対が平均30回の読み取りを示す。</dd> </dl> </div> </div> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h2>NGSプラットフォーム</h2> <h3><a href="http://www.illumina.com" target="_blank">イルミナ</a></h3> <p>イルミナは、クローン的に増幅された鋳型DNA（クラスター）上に位置する、蛍光標識された可逆的鎖ターミネーターヌクレオチドを用いた配列別合成技術を使用。 DNAクラスターは、ガラスフローセルの表面上に固定化され、 ワークフローは、4つのヌクレオチド（それぞれ異なる蛍光色素で標識された）の組み込み、4色イメージング、色素や末端基の切断、取り込み、イメージングなどを繰り返します。フローセルは大規模な並列配列決定を受ける。 この方法により、単一蛍光標識されたヌクレオチドの制御添加によるモノヌクレオチドのエラーを回避する可能性があります。 読み取りの長さは、通常約100〜150 bpです。</p> <h3><a href="http://www.lifetechnologies.com" target="_blank">イオン トレント</a></h3> <p>イオントレントは、半導体技術チップを用いて、合成中にヌクレオチドを取り込む際に放出されたプロトンを検出します。 これは、イオン球粒子と呼ばれるビーズの表面にエマルションPCR（emPCR）を使用し、リンクされた特定のアダプターを用いてDNA断片を増幅します。 各ビーズは1種類のDNA断片で覆われていて、異なるDNA断片を有するビーズは次いで、チップの陽子感知ウェル内に配置されます。 チップには一度に4つのヌクレオチドのうちの1つが浸水し、このプロセスは異なるヌクレオチドで15秒ごとに繰り返されます。 配列決定の間に4つの塩基の各々が1つずつ導入されます、組み込みの場合はプロトンが放出され、電圧信号が取り込みに比例して検出されます。.</p> <h3><a href="http://www.pacificbiosciences.com" target="_blank">パシフィック バイオサイエンス</a></h3> <p>パシフィックバイオサイエンスでは、20kbを超える塩基対の読み取りも、単一分子リアルタイム（SMRT）シーケンシングによる構造および細胞タイプの変化を観察することができます。 このプラットフォームでは、超長鎖二本鎖DNA（dsDNA）断片が、Megaruptor（登録商標）のようなDiagenode装置を用いたランダムシアリングまたは目的の標的領域の増幅によって生成されます。 SMRTbellライブラリーは、ユニバーサルヘアピンアダプターをDNA断片の各末端に連結することによって生成します。 サイズ選択条件による洗浄ステップの後、配列決定プライマーをSMRTbellテンプレートにアニーリングし、鋳型DNAに結合したDNAポリメラーゼを含む配列決定を、蛍光標識ヌクレオチドの存在下で開始。 各塩基が取り込まれると、異なる蛍光のパルスをリアルタイムで検出します。</p> <h3><a href="https://nanoporetech.com" target="_blank">オックスフォード ナノポア</a></h3> <p>Oxford Nanoporeは、単一のDNA分子配列決定に基づく技術を開発します。その技術により生物学的分子、すなわちDNAが一群の電気抵抗性高分子膜として位置するナノスケールの孔（ナノ細孔）またはその近くを通過し、イオン電流が変化します。 この変化に関する情報は、例えば4つのヌクレオチド（AまたはG r CまたはT）ならびに修飾されたヌクレオチドすべてを区別することによって分子情報に訳されます。 シーケンシングミニオンデバイスのフローセルは、数百個のナノポアチャネルのセンサアレイを含みます。 DNAサンプルは、Diagenode社のMegaruptor（登録商標）を用いてランダムシアリングによって生成され得る超長鎖DNAフラグメントが必要です。</p> <h3><a href="http://www.lifetechnologies.com/be/en/home/life-science/sequencing/next-generation-sequencing/solid-next-generation-sequencing.html" target="_blank">SOLiD</a></h3> <p>SOLiDは、ユニークな化学作用により、何千という個々のDNA分子の同時配列決定を可能にします。 それは、アダプター対ライブラリーのフラグメントが適切で、せん断されたゲノムDNAへのアダプターのライゲーションによるライブラリー作製から始まります。 次のステップでは、エマルジョンPCR（emPCR）を実施して、ビーズの表面上の個々の鋳型DNA分子をクローン的に増幅。 emPCRでは、個々の鋳型DNAをPCR試薬と混合し、水中油型エマルジョン内の疎水性シェルで囲まれた水性液滴内のプライマーコートビーズを、配列決定のためにロードするスライドガラスの表面にランダムに付着。 この技術は、シークエンシングプライマーへのライゲーションで競合する4つの蛍光標識されたジ塩基プローブのセットを使用します。</p> <h3><a href="http://454.com/products/technology.asp" target="_blank">454</a></h3> <p>454は、大規模並列パイロシーケンシングを利用しています。 始めに全ゲノムDNAまたは標的遺伝子断片の300〜800bp断片のライブラリー調製します。 次に、DNAフラグメントへのアダプターの付着および単一のDNA鎖の分離。 その後アダプターに連結されたDNAフラグメントをエマルジョンベースのクローン増幅（emPCR）で処理し、DNAライブラリーフラグメントをミクロンサイズのビーズ上に配置します。 各DNA結合ビーズを光ファイバーチップ上のウェルに入れ、器具に挿入します。 4つのDNAヌクレオチドは、配列決定操作中に固定された順序で連続して加えられ、並行して配列決定されます。</p> </div> </div> </div> </li> </ul> </div> </div>', 'in_footer' => true, 'in_menu' => true, 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The present kit incorporates the unique D-Plex technology to generate directional RNA libraries for Illumina sequencing directly from total RNAs, messenger RNAs (mRNAs) that have already been enriched by poly(A) capture, or RNAs that have already been depleted of ribosomal RNAs (rRNAs).</p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/dplex-total-rnaseq.pdf', 'slug' => 'dplex-total-rnaseq', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-04-20 14:09:33', 'created' => '2021-04-20 14:09:33', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1777', 'name' => 'product/kits/library-prep-kit-icon.png', 'alt' => 'Library prep kit icon', 'modified' => '2018-03-15 15:51:51', 'created' => '2018-03-15 15:51:26', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3401', 'name' => 'Directional high-throughput sequencing of RNAs without gene-specific primers.', 'authors' => 'Mäki A, Tiirola M', 'description' => '<p>Ribosomal RNA analysis is a useful tool for characterization of microbial communities. However, the lack of broad-range primers has hampered the simultaneous analysis of eukaryotic and prokaryotic members by amplicon sequencing. We present a complete workflow for directional, primer-independent sequencing of size-selected small subunit ribosomal RNA fragments. The library preparation protocol includes gel extraction of the target RNA, ligation of an RNA oligo to the 5'-end of the target, and cDNA synthesis with a tailed random-hexamer primer and further barcoding. The sequencing results of a phytoplankton mock community showed a highly similar profile to the biomass indicators. This method has universal potential for microbiome studies, and is compatible for the 5'-end sequencing of other RNA types with minimum library preparation costs.</p>', 'date' => '2018-10-05', 'pmid' => 'http://www.pubmed.gov/30284935', 'doi' => '10.2144/btn-2018-0082', 'modified' => '2018-12-31 11:24:16', 'created' => '2018-11-08 12:59:45', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/CATS-RNA-seq-Kit' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $pro = array( 'id' => '2881', 'antibody_id' => null, 'name' => 'CATS RNA-seq Kit v2 x96', 'description' => '<p style="text-align: justify;">Diagenode’s CATS RNA-seq Kit utilizes the innovative “<strong>C</strong>apture and <strong>A</strong>mplification by <strong>T</strong>ailing and <strong>S</strong>witching” (CATS), a <strong>ligation-free method</strong> to produce DNA libraries for next generation sequencing from low input amounts of RNA.</p> <ul> <li>Diverse transcripts detection</li> <li>Excellent reproducibility with ultra low inputs down to 100 pg</li> <li>Great performance on challenging samples</li> </ul> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#more" style="color: #13b29c; background-color: transparent; display: inline; padding: 0;">Read more</a> <div id="more" class="content"> <p style="text-align: justify;">CATS relies on polynucleotide tailing for capturing the 3’-ends of nucleic acids and the template switching ability of MMLV-RT to capture the 5’ ends for cDNA synthesis. This kit allows for generating Illumina compatible ready-to-sequence libraries from RNA inputs as low as <strong>100 picograms</strong> (<strong>rRNA depleted or polyA selected</strong>) in just a few hours.</p> <p style="text-align: justify;">Libraries retain <strong>strand specificity</strong> of origin. Unlike competing solutions, our CATS RNA-seq kit exhibits <strong>higher library efficiency</strong> versus ligation-based methods providing <strong>higher complexity</strong> from better template capture and minimal bias due to <strong>lower amplification</strong> requirements.</p> </div> </li> </ul> <div class="carrousel" style="background-position: center;"> <div class="container"> <div class="row" style="background: rgba(255,255,255,0.1); height: 241px;"> <div class="large-12 columns slick"> <div> <h3>Broad overview of the transcriptome</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/polyA-mRNA-seq-1ng-10ng .png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Biotypes* represented in the libraries <strong>CATS v2 mRNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2. <br />Input: <strong>1 ng, 10 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000). * < 0.04% of total detected transcripts at this threshold is rRNA</p> </div> </div> <div> <h3>More transcripts detected</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/rRNA-total-RNA-seq-1ng-10ng.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Biotypes* represented in the libraries <strong>CATS v2 total RNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2. <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000). * < 2% of total detected transcripts at this threshold is rRNA</p> </div> </div> <div> <h3>Uniquely detected transcripts</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_1_M1_all_CATS_NEB_nolimit_NEW.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>All detected transcripts in <strong>CATS v2 mRNA-seq Kit</strong> vs NEBNext Ultra I directional library preparation Kit. No threshold has been set for the analysis. <br />Input: <strong>1 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000). 36,245 transcripts found in both libraries.</p> </div> </div> <div> <h3>Consistency and wide representation of the transcriptome</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_2_M1_coding_CATS_SM.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Comparison on the number of coding transcripts detected with <strong>CATS v2 mRNA-seq Kit</strong> vs SMARTer stranded RNA-seq library preparation kit. TPM ≥2. <br />Input: <strong>1 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000). 21,333 transcripts found in both libraries.</p> </div> </div> <div> <h3>High-complexity at low inputs</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/high-complexity-low-inputs.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Mapped reads distribution for <strong>CATS v2 mRNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. <br />Input: <strong>1 ng, 10 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>Very reproducible library preparation method</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_4_R1_scatter_replicates_CATS.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Transcripts detected in two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>High correlation in transcripts detection across ultra low inputs</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_6_v1_M_scatter_inputs_CATS.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Transcripts detected in 1 ng vs 0.1 ng samples of <strong>CATS v2 mRNA-seq libraries. </strong>TPM≥2. <br />Input: <strong>1 ng, 0.1 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>High correlation in coding transcripts detection across different inputs</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_7_R_scatter_inputs_CATS.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Transcripts detected in 10 ng vs 1 ng samples of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>Few PCR cycles, less amplification bias</h3> <div class="large-12 small-12 medium-12 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-Fig_1ng-rRNAdplt-RNA-CATSV2.png" /></div> <div class="large-12 small-12 medium-12 columns"> <p>BioAnalyzer® electropherogram of two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>Excellent performance on challenging samples</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/Exosomes-page_heatt-1.jpg" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Heatmap clustering of the top 50 differentially expressed miRNAs after library preparation:</p> <ul> <li><strong>CATS Small RNA-seq Kit</strong> on 1 ng exosomal RNA (Exosome sample 1, 2)</li> <li><strong>CATS RNA-seq Kit</strong> on 1 ng total RNA from parent (HEK293T) cells (Cell sample 1, 2).</li> </ul> <p>Prior library preparation, exosomes were isolated with Diagenode exosome capture solutions <a href="../categories/exosomes">Exosomes</a></p> </div> </div> <div> <h3>CATS saves time</h3> <img src="https://www.diagenode.com/img/categories/library-prep/cats-save-time.png" /></div> </div> </div> </div> </div>', 'label1' => 'Characteristics', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '96 rxns', 'catalog_number' => 'C05010049', 'old_catalog_number' => '', 'sf_code' => 'C05010049-', 'type' => 'RFR', 'search_order' => '', 'price_EUR' => '4774', 'price_USD' => '4774', 'price_GBP' => '4020', 'price_JPY' => '831000', 'price_CNY' => '', 'price_AUD' => '11935', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => false, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'cats-rna-seq-kit-v2-96', 'meta_title' => 'CATS RNA-seq Kit v2 x96 | Diagenode', 'meta_keywords' => 'Capture and Amplification by Tailing and Switching(CATS), RNA-seq, non-coding RNA, long non-coding RNA, whole transcriptome, RNA-seq library preparation,higher RNA diversity, low input, easy, user-friendly', 'meta_description' => 'Capture and Amplification by Tailing and Switching RNA-seq Kit is a ligation-free assay providng increased efficiency and minimum bias, with low inputs down to 100 picograms. User-friendly with reduced hands-on time and fewer steps than competing solution', 'modified' => '2021-05-05 10:47:41', 'created' => '2017-03-07 23:18:46', 'ProductsGroup' => array( 'id' => '232', 'product_id' => '2881', 'group_id' => '207' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/D-Plex-Total-RNA-seq-Library-Prep-x24"><img src="/img/product/kits/dplex/total-dplex-1.jpg" alt="D-Plex Total RNA-seq Kit for Illumina" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C05030031</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3189" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/3189" id="CartAdd/3189Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3189" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> D-Plex Total RNA-seq Kit for Illumina</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('D-Plex Total RNA-seq Kit for Illumina', 'C05030031', '1475', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('D-Plex Total RNA-seq Kit for Illumina', 'C05030031', '1475', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="D-Plex-Total-RNA-seq-Library-Prep-x24" data-reveal-id="cartModal-3189" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">D-Plex Total RNA-seq Kit for Illumina</h6> </div> </div> </li> ' $related = array( 'id' => '3189', 'antibody_id' => null, 'name' => 'D-Plex Total RNA-seq Kit for Illumina', 'description' => '<div class="small-12 medium-12 large-12 columns" style="border: 3px solid #B02736; padding: 10px; margin: 10px;"> <h2>Product Features</h2> <ul style="list-style-type: disc;"> <li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li> <li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li> <li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li> <li><span>Get high sensitivity data even from difficult samples, such as degraded, FFPE samples</span></li> <li><span style="font-weight: 400;">Enjoy a fast, easy, single tube protocol</span></li> </ul> </div> <p></p> <p></p> <h4></h4> <center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center> <p></p> <p>D-Plex Total RNA-seq Library Preparation Kit is a tool designed for the study of the whole coding and non-coding transcriptome. <span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span> </span>D-Plex technology</a><span><span> </span>to generate directional libraries for Illumina sequencing directly from total RNAs, mRNAs that has already been enriched by poly(A) selection, or RNAs that has already been depleted of rRNAs.</span></p> <p><span>The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p> <p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. <span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.</span></span></p> <p><span><span>D-Plex Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p> <ul> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C"><span>C05030023</span> - D-Plex Unique Dual Indexes for Illumina - Set C </a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span> - D-Plex Unique Dual Indexes for Illumina - Set D </a></li> </ul> <div class="extra-spaced" align="center"></div> <div class="row"> <div class="carrousel" style="background-position: center;"> <div class="slick"> <div> <h3>Greater sensitivity to detect novel transcripts</h3> <div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div> <div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"> <p></p> <p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p> </div> </div> <div> <h3>Highest diversity to facilitate biomarker discovery</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p> </div> </div> <div> <h3>Consistent expression profiling across a wide range of RNA inputs</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p> </div> </div> <div> <h3>Superior library complexity at low RNA inputs</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p> </div> </div> </div> </div> </div>', 'label1' => '', 'info1' => '', 'label2' => 'Indexes', 'info2' => '<p><span>Specific D-Plex indexes </span><span>were designed and validated to fit the D-Plex technology for Illumina sequencing and </span><span>are not included in the kit. They can be bought separately according to your needs. Please choose the format that suits you best among the compatible references to:</span></p> <ul> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C" title="D-Plex UDI Module - Set C" target="_blank"><span>C05030023</span> - D-Plex Unique Dual Indexes for Illumina - Set C</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span> - D-Plex Unique Dual Indexes for Illumina - Set D </a></li> </ul> <p></p> <p>The use of UDI is highly recommended to mitigate errors introduced by read misassignment, including index hopping frequently observed with patterned flow cells such as Illumina’s NovaSeq system.</p>', 'label3' => 'Data analysis', 'info3' => '<p><span>A specific bioinformatics pipeline has been developed to process the special sequences present in the D-Plex construct, namely the UMI, the A-tail, and the template switch motif. 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Subject to the compatibility of D-Plex constructs, other specific pipelines can be used.</span></p> <p><img src="https://www.diagenode.com/img/product/kits/dplex/bioinfoPipe.png" alt="Small RNA seq Bioinformatics pipeline" width="925" height="196" /></p>', 'format' => '24 rxns', 'catalog_number' => 'C05030031', 'old_catalog_number' => '', 'sf_code' => 'C05030031-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '1475', 'price_USD' => '1475', 'price_GBP' => '1355', 'price_JPY' => '231060', 'price_CNY' => '', 'price_AUD' => '3688', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'D-Plex-Total-RNA-seq-Library-Prep-x24', 'meta_title' => 'D-Plex Total RNA-seq Library Prep Kit for Illumina | Diagenode ', 'meta_keywords' => 'RNA-seq kit, RNA-seq library preparation; low input RNA-seq, UMI RNA-seq, UDI RNA-seq, D-Plex, higher RNA sensitivity', 'meta_description' => 'RNA-seq library preparation for Illumina sequencing - Unique D-Plex technology - Optimized for ultra-low input (50 pg total RNA) - UMI reduce PCR biases - UDI detect index hopping - Compatibility with FFPE samples - User-friendly and fast protocol', 'modified' => '2023-11-16 09:09:25', 'created' => '2021-04-13 14:10:40', 'ProductsRelated' => array( 'id' => '4753', 'product_id' => '2844', 'related_id' => '3189' ), 'Image' => array( (int) 0 => array( 'id' => '1817', 'name' => 'product/kits/dplex/total-dplex-1.jpg', 'alt' => 'D-Plex Total RNA-seq Kit for Illumina', 'modified' => '2021-05-11 16:42:58', 'created' => '2021-05-11 16:42:58', 'ProductsImage' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1818', 'name' => 'product/kits/dplex/total-dplex-2.jpg', 'alt' => 'D-Plex Total RNA-seq Kit for Illumina', 'modified' => '2021-05-11 16:43:26', 'created' => '2021-05-11 16:43:26', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '1134', 'name' => 'D-Plex Total RNA-seq Kit', 'description' => '<p class="p1">The Diagenode D-Plex Total RNA-seq Library Preparation kit is a tool designed for the study of the whole coding and non-coding transcriptome. The present kit incorporates the unique D-Plex technology to generate directional RNA libraries for Illumina sequencing directly from total RNAs, messenger RNAs (mRNAs) that have already been enriched by poly(A) capture, or RNAs that have already been depleted of ribosomal RNAs (rRNAs).</p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/dplex-total-rnaseq.pdf', 'slug' => 'dplex-total-rnaseq', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-04-20 14:09:33', 'created' => '2021-04-20 14:09:33', 'ProductsDocument' => array( 'id' => '3122', 'product_id' => '2844', 'document_id' => '1134' ) ) $publication = array( 'id' => '3401', 'name' => 'Directional high-throughput sequencing of RNAs without gene-specific primers.', 'authors' => 'Mäki A, Tiirola M', 'description' => '<p>Ribosomal RNA analysis is a useful tool for characterization of microbial communities. 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User-friendly with reduced hands-on time and fewer steps than competing solution', 'modified' => '2021-05-07 15:31:50', 'created' => '2016-10-21 06:18:57' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '3189', 'antibody_id' => null, 'name' => 'D-Plex Total RNA-seq Kit for Illumina', 'description' => '<div class="small-12 medium-12 large-12 columns" style="border: 3px solid #B02736; padding: 10px; margin: 10px;"> <h2>Product Features</h2> <ul style="list-style-type: disc;"> <li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li> <li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li> <li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li> <li><span>Get high sensitivity data even from difficult samples, such as degraded, FFPE samples</span></li> <li><span style="font-weight: 400;">Enjoy a fast, easy, single tube protocol</span></li> </ul> </div> <p></p> <p></p> <h4></h4> <center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center> <p></p> <p>D-Plex Total RNA-seq Library Preparation Kit is a tool designed for the study of the whole coding and non-coding transcriptome. <span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span> </span>D-Plex technology</a><span><span> </span>to generate directional libraries for Illumina sequencing directly from total RNAs, mRNAs that has already been enriched by poly(A) selection, or RNAs that has already been depleted of rRNAs.</span></p> <p><span>The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p> <p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. <span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.</span></span></p> <p><span><span>D-Plex Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p> <ul> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C"><span>C05030023</span> - D-Plex Unique Dual Indexes for Illumina - Set C </a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span> - D-Plex Unique Dual Indexes for Illumina - Set D </a></li> </ul> <div class="extra-spaced" align="center"></div> <div class="row"> <div class="carrousel" style="background-position: center;"> <div class="slick"> <div> <h3>Greater sensitivity to detect novel transcripts</h3> <div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div> <div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"> <p></p> <p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p> </div> </div> <div> <h3>Highest diversity to facilitate biomarker discovery</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p> </div> </div> <div> <h3>Consistent expression profiling across a wide range of RNA inputs</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p> </div> </div> <div> <h3>Superior library complexity at low RNA inputs</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p> </div> </div> </div> </div> </div>', 'label1' => '', 'info1' => '', 'label2' => 'Indexes', 'info2' => '<p><span>Specific D-Plex indexes </span><span>were designed and validated to fit the D-Plex technology for Illumina sequencing and </span><span>are not included in the kit. They can be bought separately according to your needs. Please choose the format that suits you best among the compatible references to:</span></p> <ul> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C" title="D-Plex UDI Module - Set C" target="_blank"><span>C05030023</span> - D-Plex Unique Dual Indexes for Illumina - Set C</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span> - D-Plex Unique Dual Indexes for Illumina - Set D </a></li> </ul> <p></p> <p>The use of UDI is highly recommended to mitigate errors introduced by read misassignment, including index hopping frequently observed with patterned flow cells such as Illumina’s NovaSeq system.</p>', 'label3' => 'Data analysis', 'info3' => '<p><span>A specific bioinformatics pipeline has been developed to process the special sequences present in the D-Plex construct, namely the UMI, the A-tail, and the template switch motif. All guidelines and free softwares are shared in the user manual. Subject to the compatibility of D-Plex constructs, other specific pipelines can be used.</span></p> <p><img src="https://www.diagenode.com/img/product/kits/dplex/bioinfoPipe.png" alt="Small RNA seq Bioinformatics pipeline" width="925" height="196" /></p>', 'format' => '24 rxns', 'catalog_number' => 'C05030031', 'old_catalog_number' => '', 'sf_code' => 'C05030031-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '1475', 'price_USD' => '1475', 'price_GBP' => '1355', 'price_JPY' => '231060', 'price_CNY' => '', 'price_AUD' => '3688', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'D-Plex-Total-RNA-seq-Library-Prep-x24', 'meta_title' => 'D-Plex Total RNA-seq Library Prep Kit for Illumina | Diagenode ', 'meta_keywords' => 'RNA-seq kit, RNA-seq library preparation; low input RNA-seq, UMI RNA-seq, UDI RNA-seq, D-Plex, higher RNA sensitivity', 'meta_description' => 'RNA-seq library preparation for Illumina sequencing - Unique D-Plex technology - Optimized for ultra-low input (50 pg total RNA) - UMI reduce PCR biases - UDI detect index hopping - Compatibility with FFPE samples - User-friendly and fast protocol', 'modified' => '2023-11-16 09:09:25', 'created' => '2021-04-13 14:10:40', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '14', 'position' => '10', 'parent_id' => '3', 'name' => 'DNA/RNA library preparation', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><span style="font-weight: 400;">Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to </span><a href="../applications/dna-rna-shearing"><span style="font-weight: 400;">fragmented DNA or RNA</span></a><span style="font-weight: 400;"> prior to sequencing</span></p> <p><span style="font-weight: 400;">After input DNA has been fragmented, it is end-repaired and blunt-ended</span><span style="font-weight: 400;">. The next step is a A-tailing in which dAMP is added to the 3´ end of the blunt phosphorylated DNA fragments to prevent concatemerization and to allow the ligation of adaptors with complementary dT overhangs. In addition, barcoded adapters can be incorporated to facilitate multiplexing prior to or during amplification.</span></p> <center><img src="https://www.diagenode.com/img/categories/library-prep/flux.png" /></center> <p><span style="font-weight: 400;">Diagenode offers a comprehensive product portfolio for library preparation:<br /></span></p> <strong><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex RNA-seq Library Preparation Kits</a></strong><br /> <p><span style="font-weight: 400;">Diagenode’s new RNA-sequencing solutions utilize the innovative c</span><span style="font-weight: 400;">apture and a</span><span style="font-weight: 400;">mplification by t</span><span style="font-weight: 400;">ailing and s</span><span style="font-weight: 400;">witching”</span><span style="font-weight: 400;">, a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of RNA. </span><span style="font-weight: 400;"></span><a href="../categories/Library-preparation-for-RNA-seq">Learn more</a></p> <strong><a href="../categories/library-preparation-for-ChIP-seq">ChIP-seq and DNA sequencing library preparation solutions</a></strong><br /> <p><span style="font-weight: 400;">Our kits have been optimized for DNA library preparation used for next generation sequencing for a wide range of inputs. Using a simple three-step protocols, our</span><a href="http://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><span style="font-weight: 400;"> </span></a><span style="font-weight: 400;">kits are an optimal choice for library preparation from DNA inputs down to 50 pg. </span><a href="../categories/library-preparation-for-ChIP-seq">Learn more</a></p> <a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span><strong>Bioruptor Pico - short fragments</strong></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br /> <p><span style="font-weight: 400;"></span><span style="font-weight: 400;">Our well-cited Bioruptor Pico is the shearing device of choice for chromatin and DNA fragmentation. Obtain uniform and tight fragment distributions between 150bp -2kb. </span><a href="../p/bioruptor-pico-sonication-device">Learn more</a></p> <strong><a href="../p/megaruptor2-1-unit"><span href="../p/bioruptor-pico-sonication-device">Megaruptor</span>® - long fragments</a></strong><a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br /> <p><span style="font-weight: 400;"></span><span style="font-weight: 400;">The Megaruptor is designed to shear DNA from 3kb-75kb for long-read sequencing. <a href="../p/megaruptor2-1-unit">Learn more</a></span></p> <span href="../p/bioruptor-pico-sonication-device"></span><span style="font-weight: 400;"></span></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'library-preparation', 'meta_keywords' => 'Library preparation,Next Generation Sequencing,DNA fragments,MicroPlex Library,iDeal Library', 'meta_description' => 'Diagenode offers a comprehensive product portfolio for library preparation such as CATS RNA-seq Library Preparation Kits,ChIP-seq and DNA sequencing library preparation solutions', 'meta_title' => 'Library preparation for Next Generation Sequencing (NGS) | Diagenode', 'modified' => '2021-03-10 03:27:16', 'created' => '2014-08-16 07:47:56', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3', 'position' => '10', 'parent_id' => null, 'name' => '次世代シーケンシング', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns"> <h2 style="font-size: 22px;">DNA断片化、ライブラリー調製、自動化：NGSのワンストップショップ</h2> <table class="small-12 medium-12 large-12 columns"> <tbody> <tr> <th class="small-12 medium-12 large-12 columns"> <h4>1. 断片化装置を選択してください：150 bp〜75 kbの範囲でDNAを断片化します。</h4> </th> </tr> <tr style="background-color: #ffffff;"> <td class="small-12 medium-12 large-12 columns"></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-pico-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/bioruptor_pico.jpg" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/megaruptor2-1-unit"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010001_megaruptor2.jpg" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-one-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-profil.png" /></a></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns">5μlまで断片化：150 bp〜2 kb<br />NGS DNAライブラリー調製およびFFPE核酸抽出に最適で、</td> <td class="small-4 medium-4 large-4 columns">2 kb〜75 kbの範囲をできます。<br />メイトペアライブラリー調製および長いフラグメントDNAシーケンシングに最適で、この軽量デスクトップデバイスで</td> <td class="small-4 medium-4 large-4 columns">20または50μlの断片化が可能です。</td> </tr> </tbody> </table> <table class="small-12 medium-12 large-12 columns"> <tbody> <tr> <th class="small-8 medium-8 large-8 columns"> <h4>2. 最適化されたライブラリー調整キットを選択してください。</h4> </th> <th class="small-4 medium-4 large-4 columns"> <h4>3. ライブラリー前処理自動化を選択して、比類のないデータ再現性を実感</h4> </th> </tr> <tr style="background-color: #ffffff;"> <td class="small-12 medium-12 large-12 columns"></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns"><a href="../p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><img src="https://www.diagenode.com/img/product/kits/microPlex_library_preparation.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/ideal-library-preparation-kit-x24-incl-index-primer-set-1-24-rxns"><img src="https://www.diagenode.com/img/product/kits/box_kit.jpg" style="display: block; margin-left: auto; margin-right: auto;" height="173" width="250" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/sx-8g-ip-star-compact-automated-system-1-unit"><img src="https://www.diagenode.com/img/product/automation/B03000002%20_ipstar_compact.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">50pgの低入力：MicroPlex Library Preparation Kit</td> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">5ng以上：iDeal Library Preparation Kit</td> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">Achieve great NGS data easily</td> </tr> </tbody> </table> </div> </div> <blockquote> <div class="row"> <div class="small-12 medium-12 large-12 columns"><span class="label" style="margin-bottom: 16px; margin-left: -22px; font-size: 15px;">DiagenodeがNGS研究にぴったりなプロバイダーである理由</span> <p>Diagenodeは15年以上もエピジェネティクス研究に専念、専門としています。 ChIP研究クロマチン用のユニークな断片化システムの開発から始まり、 専門知識を活かし、5μlのせん断体積まで可能で、NGS DNAライブラリーの調製に最適な最先端DNA断片化装置の開発にたどり着きました。 我々は以来、ChIP-seq、Methyl-seq、NGSライブラリー調製用キットを研究開発し、業界をリードする免疫沈降研究と同様に、ライブラリー調製を自動化および完結させる独自の自動化システムを開発にも成功しました。</p> <ul> <li>信頼されるせん断装置</li> <li>様々なインプットからのライブラリ作成キット</li> <li>独自の自動化デバイス</li> </ul> </div> </div> </blockquote> <div class="row"> <div class="small-12 columns"> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#panel1a">次世代シーケンシングへの理解とその専門知識</a> <div id="panel1a" class="content"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong>次世代シーケンシング (NGS)</strong> ）は、著しいスケールとハイスループットでシーケンシングを行い、1日に数十億もの塩基生成を可能にします。 NGSのハイスループットは迅速でありながら正確で、再現性のあるデータセットを実現し、さらにシーケンシング費用を削減します。 NGSは、ゲノムシーケンシング、ゲノム再シーケンシング、デノボシーケンシング、トランスクリプトームシーケンシング、その他にDNA-タンパク質相互作用の検出やエピゲノムなどを示します。 指数関数的に増加するシーケンシングデータの需要は、計算分析の障害や解釈、データストレージなどの課題を解決します。</p> <p>アプリケーションおよび出発物質に応じて、数百万から数十億の鋳型DNA分子を大規模に並行してシーケンシングすることが可能です。その為に、異なる化学物質を使用するいくつかの市販のNGSプラットフォームを利用することができます。 NGSプラットフォームの種類によっては、事前準備とライブラリー作成が必要です。</p> <p>NGSにとっても、特にデータ処理と分析に関した大きな課題はあります。第3世代技術はゲノミクス研究にさらに革命を起こすであろうと大きく期待されています。</p> </div> </div> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <p><strong>NGS アプリケーション</strong></p> <ul> <li>全ゲノム配列決定</li> <li>デノボシーケンシング</li> <li>標的配列</li> <li>Exomeシーケンシング</li> <li>トランスクリプトーム配列決定</li> <li>ゲノム配列決定</li> <li>ミトコンドリア配列決定</li> <li>DNA-タンパク質相互作用（ChIP-seq</li> <li>バリアント検出</li> <li>ゲノム仕上げ</li> </ul> </div> <div class="small-6 medium-6 large-6 columns"> <p><strong>研究分野におけるNGS:</strong></p> <ul> <li>腫瘍学</li> <li>リプロダクティブ・ヘルス</li> <li>法医学ゲノミクス</li> <li>アグリゲノミックス</li> <li>複雑な病気</li> <li>微生物ゲノミクス</li> <li>食品・環境ゲノミクス</li> <li>創薬ゲノミクス - パーソナライズド・メディカル</li> </ul> </div> <div class="small-12 medium-12 large-12 columns"> <p><strong>NGSの用語</strong></p> <dl> <dt>リード（読み取り）</dt> <dd>この装置から得られた連続した単一のストレッチ</dd> <dt>断片リード</dt> <dd>フラグメントライブラリからの読み込み。 シーケンシングプラットフォームに応じて、読み取りは通常約100〜300bp。</dd> <dt>断片ペアエンドリード</dt> <dd>断片ライブラリーからDNA断片の各末端2つの読み取り。</dd> <dt>メイトペアリード</dt> <dd>大きなDNA断片（通常は予め定義されたサイズ範囲）の各末端から2つの読み取り。</dd> <dt>カバレッジ（例）</dt> <dd>30×適用範囲とは、参照ゲノム中の各塩基対が平均30回の読み取りを示す。</dd> </dl> </div> </div> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h2>NGSプラットフォーム</h2> <h3><a href="http://www.illumina.com" target="_blank">イルミナ</a></h3> <p>イルミナは、クローン的に増幅された鋳型DNA（クラスター）上に位置する、蛍光標識された可逆的鎖ターミネーターヌクレオチドを用いた配列別合成技術を使用。 DNAクラスターは、ガラスフローセルの表面上に固定化され、 ワークフローは、4つのヌクレオチド（それぞれ異なる蛍光色素で標識された）の組み込み、4色イメージング、色素や末端基の切断、取り込み、イメージングなどを繰り返します。フローセルは大規模な並列配列決定を受ける。 この方法により、単一蛍光標識されたヌクレオチドの制御添加によるモノヌクレオチドのエラーを回避する可能性があります。 読み取りの長さは、通常約100〜150 bpです。</p> <h3><a href="http://www.lifetechnologies.com" target="_blank">イオン トレント</a></h3> <p>イオントレントは、半導体技術チップを用いて、合成中にヌクレオチドを取り込む際に放出されたプロトンを検出します。 これは、イオン球粒子と呼ばれるビーズの表面にエマルションPCR（emPCR）を使用し、リンクされた特定のアダプターを用いてDNA断片を増幅します。 各ビーズは1種類のDNA断片で覆われていて、異なるDNA断片を有するビーズは次いで、チップの陽子感知ウェル内に配置されます。 チップには一度に4つのヌクレオチドのうちの1つが浸水し、このプロセスは異なるヌクレオチドで15秒ごとに繰り返されます。 配列決定の間に4つの塩基の各々が1つずつ導入されます、組み込みの場合はプロトンが放出され、電圧信号が取り込みに比例して検出されます。.</p> <h3><a href="http://www.pacificbiosciences.com" target="_blank">パシフィック バイオサイエンス</a></h3> <p>パシフィックバイオサイエンスでは、20kbを超える塩基対の読み取りも、単一分子リアルタイム（SMRT）シーケンシングによる構造および細胞タイプの変化を観察することができます。 このプラットフォームでは、超長鎖二本鎖DNA（dsDNA）断片が、Megaruptor（登録商標）のようなDiagenode装置を用いたランダムシアリングまたは目的の標的領域の増幅によって生成されます。 SMRTbellライブラリーは、ユニバーサルヘアピンアダプターをDNA断片の各末端に連結することによって生成します。 サイズ選択条件による洗浄ステップの後、配列決定プライマーをSMRTbellテンプレートにアニーリングし、鋳型DNAに結合したDNAポリメラーゼを含む配列決定を、蛍光標識ヌクレオチドの存在下で開始。 各塩基が取り込まれると、異なる蛍光のパルスをリアルタイムで検出します。</p> <h3><a href="https://nanoporetech.com" target="_blank">オックスフォード ナノポア</a></h3> <p>Oxford Nanoporeは、単一のDNA分子配列決定に基づく技術を開発します。その技術により生物学的分子、すなわちDNAが一群の電気抵抗性高分子膜として位置するナノスケールの孔（ナノ細孔）またはその近くを通過し、イオン電流が変化します。 この変化に関する情報は、例えば4つのヌクレオチド（AまたはG r CまたはT）ならびに修飾されたヌクレオチドすべてを区別することによって分子情報に訳されます。 シーケンシングミニオンデバイスのフローセルは、数百個のナノポアチャネルのセンサアレイを含みます。 DNAサンプルは、Diagenode社のMegaruptor（登録商標）を用いてランダムシアリングによって生成され得る超長鎖DNAフラグメントが必要です。</p> <h3><a href="http://www.lifetechnologies.com/be/en/home/life-science/sequencing/next-generation-sequencing/solid-next-generation-sequencing.html" target="_blank">SOLiD</a></h3> <p>SOLiDは、ユニークな化学作用により、何千という個々のDNA分子の同時配列決定を可能にします。 それは、アダプター対ライブラリーのフラグメントが適切で、せん断されたゲノムDNAへのアダプターのライゲーションによるライブラリー作製から始まります。 次のステップでは、エマルジョンPCR（emPCR）を実施して、ビーズの表面上の個々の鋳型DNA分子をクローン的に増幅。 emPCRでは、個々の鋳型DNAをPCR試薬と混合し、水中油型エマルジョン内の疎水性シェルで囲まれた水性液滴内のプライマーコートビーズを、配列決定のためにロードするスライドガラスの表面にランダムに付着。 この技術は、シークエンシングプライマーへのライゲーションで競合する4つの蛍光標識されたジ塩基プローブのセットを使用します。</p> <h3><a href="http://454.com/products/technology.asp" target="_blank">454</a></h3> <p>454は、大規模並列パイロシーケンシングを利用しています。 始めに全ゲノムDNAまたは標的遺伝子断片の300〜800bp断片のライブラリー調製します。 次に、DNAフラグメントへのアダプターの付着および単一のDNA鎖の分離。 その後アダプターに連結されたDNAフラグメントをエマルジョンベースのクローン増幅（emPCR）で処理し、DNAライブラリーフラグメントをミクロンサイズのビーズ上に配置します。 各DNA結合ビーズを光ファイバーチップ上のウェルに入れ、器具に挿入します。 4つのDNAヌクレオチドは、配列決定操作中に固定された順序で連続して加えられ、並行して配列決定されます。</p> </div> </div> </div> </li> </ul> </div> </div>', 'in_footer' => true, 'in_menu' => true, 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The present kit incorporates the unique D-Plex technology to generate directional RNA libraries for Illumina sequencing directly from total RNAs, messenger RNAs (mRNAs) that have already been enriched by poly(A) capture, or RNAs that have already been depleted of ribosomal RNAs (rRNAs).</p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/dplex-total-rnaseq.pdf', 'slug' => 'dplex-total-rnaseq', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-04-20 14:09:33', 'created' => '2021-04-20 14:09:33', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1777', 'name' => 'product/kits/library-prep-kit-icon.png', 'alt' => 'Library prep kit icon', 'modified' => '2018-03-15 15:51:51', 'created' => '2018-03-15 15:51:26', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3401', 'name' => 'Directional high-throughput sequencing of RNAs without gene-specific primers.', 'authors' => 'Mäki A, Tiirola M', 'description' => '<p>Ribosomal RNA analysis is a useful tool for characterization of microbial communities. However, the lack of broad-range primers has hampered the simultaneous analysis of eukaryotic and prokaryotic members by amplicon sequencing. We present a complete workflow for directional, primer-independent sequencing of size-selected small subunit ribosomal RNA fragments. The library preparation protocol includes gel extraction of the target RNA, ligation of an RNA oligo to the 5'-end of the target, and cDNA synthesis with a tailed random-hexamer primer and further barcoding. The sequencing results of a phytoplankton mock community showed a highly similar profile to the biomass indicators. This method has universal potential for microbiome studies, and is compatible for the 5'-end sequencing of other RNA types with minimum library preparation costs.</p>', 'date' => '2018-10-05', 'pmid' => 'http://www.pubmed.gov/30284935', 'doi' => '10.2144/btn-2018-0082', 'modified' => '2018-12-31 11:24:16', 'created' => '2018-11-08 12:59:45', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/CATS-RNA-seq-Kit' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $pro = array( 'id' => '2881', 'antibody_id' => null, 'name' => 'CATS RNA-seq Kit v2 x96', 'description' => '<p style="text-align: justify;">Diagenode’s CATS RNA-seq Kit utilizes the innovative “<strong>C</strong>apture and <strong>A</strong>mplification by <strong>T</strong>ailing and <strong>S</strong>witching” (CATS), a <strong>ligation-free method</strong> to produce DNA libraries for next generation sequencing from low input amounts of RNA.</p> <ul> <li>Diverse transcripts detection</li> <li>Excellent reproducibility with ultra low inputs down to 100 pg</li> <li>Great performance on challenging samples</li> </ul> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#more" style="color: #13b29c; background-color: transparent; display: inline; padding: 0;">Read more</a> <div id="more" class="content"> <p style="text-align: justify;">CATS relies on polynucleotide tailing for capturing the 3’-ends of nucleic acids and the template switching ability of MMLV-RT to capture the 5’ ends for cDNA synthesis. This kit allows for generating Illumina compatible ready-to-sequence libraries from RNA inputs as low as <strong>100 picograms</strong> (<strong>rRNA depleted or polyA selected</strong>) in just a few hours.</p> <p style="text-align: justify;">Libraries retain <strong>strand specificity</strong> of origin. Unlike competing solutions, our CATS RNA-seq kit exhibits <strong>higher library efficiency</strong> versus ligation-based methods providing <strong>higher complexity</strong> from better template capture and minimal bias due to <strong>lower amplification</strong> requirements.</p> </div> </li> </ul> <div class="carrousel" style="background-position: center;"> <div class="container"> <div class="row" style="background: rgba(255,255,255,0.1); height: 241px;"> <div class="large-12 columns slick"> <div> <h3>Broad overview of the transcriptome</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/polyA-mRNA-seq-1ng-10ng .png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Biotypes* represented in the libraries <strong>CATS v2 mRNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2. <br />Input: <strong>1 ng, 10 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000). * < 0.04% of total detected transcripts at this threshold is rRNA</p> </div> </div> <div> <h3>More transcripts detected</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/rRNA-total-RNA-seq-1ng-10ng.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Biotypes* represented in the libraries <strong>CATS v2 total RNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2. <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000). * < 2% of total detected transcripts at this threshold is rRNA</p> </div> </div> <div> <h3>Uniquely detected transcripts</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_1_M1_all_CATS_NEB_nolimit_NEW.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>All detected transcripts in <strong>CATS v2 mRNA-seq Kit</strong> vs NEBNext Ultra I directional library preparation Kit. No threshold has been set for the analysis. <br />Input: <strong>1 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000). 36,245 transcripts found in both libraries.</p> </div> </div> <div> <h3>Consistency and wide representation of the transcriptome</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_2_M1_coding_CATS_SM.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Comparison on the number of coding transcripts detected with <strong>CATS v2 mRNA-seq Kit</strong> vs SMARTer stranded RNA-seq library preparation kit. TPM ≥2. <br />Input: <strong>1 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000). 21,333 transcripts found in both libraries.</p> </div> </div> <div> <h3>High-complexity at low inputs</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/high-complexity-low-inputs.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Mapped reads distribution for <strong>CATS v2 mRNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. <br />Input: <strong>1 ng, 10 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>Very reproducible library preparation method</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_4_R1_scatter_replicates_CATS.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Transcripts detected in two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>High correlation in transcripts detection across ultra low inputs</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_6_v1_M_scatter_inputs_CATS.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Transcripts detected in 1 ng vs 0.1 ng samples of <strong>CATS v2 mRNA-seq libraries. </strong>TPM≥2. <br />Input: <strong>1 ng, 0.1 ng</strong> poly(A) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>High correlation in coding transcripts detection across different inputs</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_7_R_scatter_inputs_CATS.png" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Transcripts detected in 10 ng vs 1 ng samples of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>Few PCR cycles, less amplification bias</h3> <div class="large-12 small-12 medium-12 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-Fig_1ng-rRNAdplt-RNA-CATSV2.png" /></div> <div class="large-12 small-12 medium-12 columns"> <p>BioAnalyzer® electropherogram of two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p> </div> </div> <div> <h3>Excellent performance on challenging samples</h3> <div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/Exosomes-page_heatt-1.jpg" /></div> <div class="large-5 small-12 medium-5 columns"> <p>Heatmap clustering of the top 50 differentially expressed miRNAs after library preparation:</p> <ul> <li><strong>CATS Small RNA-seq Kit</strong> on 1 ng exosomal RNA (Exosome sample 1, 2)</li> <li><strong>CATS RNA-seq Kit</strong> on 1 ng total RNA from parent (HEK293T) cells (Cell sample 1, 2).</li> </ul> <p>Prior library preparation, exosomes were isolated with Diagenode exosome capture solutions <a href="../categories/exosomes">Exosomes</a></p> </div> </div> <div> <h3>CATS saves time</h3> <img src="https://www.diagenode.com/img/categories/library-prep/cats-save-time.png" /></div> </div> </div> </div> </div>', 'label1' => 'Characteristics', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '96 rxns', 'catalog_number' => 'C05010049', 'old_catalog_number' => '', 'sf_code' => 'C05010049-', 'type' => 'RFR', 'search_order' => '', 'price_EUR' => '4774', 'price_USD' => '4774', 'price_GBP' => '4020', 'price_JPY' => '831000', 'price_CNY' => '', 'price_AUD' => '11935', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => false, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'cats-rna-seq-kit-v2-96', 'meta_title' => 'CATS RNA-seq Kit v2 x96 | Diagenode', 'meta_keywords' => 'Capture and Amplification by Tailing and Switching(CATS), RNA-seq, non-coding RNA, long non-coding RNA, whole transcriptome, RNA-seq library preparation,higher RNA diversity, low input, easy, user-friendly', 'meta_description' => 'Capture and Amplification by Tailing and Switching RNA-seq Kit is a ligation-free assay providng increased efficiency and minimum bias, with low inputs down to 100 picograms. User-friendly with reduced hands-on time and fewer steps than competing solution', 'modified' => '2021-05-05 10:47:41', 'created' => '2017-03-07 23:18:46', 'ProductsGroup' => array( 'id' => '232', 'product_id' => '2881', 'group_id' => '207' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/D-Plex-Total-RNA-seq-Library-Prep-x24"><img src="/img/product/kits/dplex/total-dplex-1.jpg" alt="D-Plex Total RNA-seq Kit for Illumina" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C05030031</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3189" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/3189" id="CartAdd/3189Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3189" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> D-Plex Total RNA-seq Kit for Illumina</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('D-Plex Total RNA-seq Kit for Illumina', 'C05030031', '1475', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('D-Plex Total RNA-seq Kit for Illumina', 'C05030031', '1475', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="D-Plex-Total-RNA-seq-Library-Prep-x24" data-reveal-id="cartModal-3189" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">D-Plex Total RNA-seq Kit for Illumina</h6> </div> </div> </li> ' $related = array( 'id' => '3189', 'antibody_id' => null, 'name' => 'D-Plex Total RNA-seq Kit for Illumina', 'description' => '<div class="small-12 medium-12 large-12 columns" style="border: 3px solid #B02736; padding: 10px; margin: 10px;"> <h2>Product Features</h2> <ul style="list-style-type: disc;"> <li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li> <li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li> <li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li> <li><span>Get high sensitivity data even from difficult samples, such as degraded, FFPE samples</span></li> <li><span style="font-weight: 400;">Enjoy a fast, easy, single tube protocol</span></li> </ul> </div> <p></p> <p></p> <h4></h4> <center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center> <p></p> <p>D-Plex Total RNA-seq Library Preparation Kit is a tool designed for the study of the whole coding and non-coding transcriptome. <span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span> </span>D-Plex technology</a><span><span> </span>to generate directional libraries for Illumina sequencing directly from total RNAs, mRNAs that has already been enriched by poly(A) selection, or RNAs that has already been depleted of rRNAs.</span></p> <p><span>The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p> <p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. <span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.</span></span></p> <p><span><span>D-Plex Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p> <ul> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C"><span>C05030023</span> - D-Plex Unique Dual Indexes for Illumina - Set C </a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span> - D-Plex Unique Dual Indexes for Illumina - Set D </a></li> </ul> <div class="extra-spaced" align="center"></div> <div class="row"> <div class="carrousel" style="background-position: center;"> <div class="slick"> <div> <h3>Greater sensitivity to detect novel transcripts</h3> <div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div> <div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"> <p></p> <p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p> </div> </div> <div> <h3>Highest diversity to facilitate biomarker discovery</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p> </div> </div> <div> <h3>Consistent expression profiling across a wide range of RNA inputs</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p> </div> </div> <div> <h3>Superior library complexity at low RNA inputs</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"> <p></p> <p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p> </div> </div> </div> </div> </div>', 'label1' => '', 'info1' => '', 'label2' => 'Indexes', 'info2' => '<p><span>Specific D-Plex indexes </span><span>were designed and validated to fit the D-Plex technology for Illumina sequencing and </span><span>are not included in the kit. They can be bought separately according to your needs. Please choose the format that suits you best among the compatible references to:</span></p> <ul> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C" title="D-Plex UDI Module - Set C" target="_blank"><span>C05030023</span> - D-Plex Unique Dual Indexes for Illumina - Set C</a></li> <li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span> - D-Plex Unique Dual Indexes for Illumina - Set D </a></li> </ul> <p></p> <p>The use of UDI is highly recommended to mitigate errors introduced by read misassignment, including index hopping frequently observed with patterned flow cells such as Illumina’s NovaSeq system.</p>', 'label3' => 'Data analysis', 'info3' => '<p><span>A specific bioinformatics pipeline has been developed to process the special sequences present in the D-Plex construct, namely the UMI, the A-tail, and the template switch motif. 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The present kit incorporates the unique D-Plex technology to generate directional RNA libraries for Illumina sequencing directly from total RNAs, messenger RNAs (mRNAs) that have already been enriched by poly(A) capture, or RNAs that have already been depleted of ribosomal RNAs (rRNAs).</p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/dplex-total-rnaseq.pdf', 'slug' => 'dplex-total-rnaseq', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-04-20 14:09:33', 'created' => '2021-04-20 14:09:33', 'ProductsDocument' => array( 'id' => '3122', 'product_id' => '2844', 'document_id' => '1134' ) ) $publication = array( 'id' => '3401', 'name' => 'Directional high-throughput sequencing of RNAs without gene-specific primers.', 'authors' => 'Mäki A, Tiirola M', 'description' => '<p>Ribosomal RNA analysis is a useful tool for characterization of microbial communities. However, the lack of broad-range primers has hampered the simultaneous analysis of eukaryotic and prokaryotic members by amplicon sequencing. We present a complete workflow for directional, primer-independent sequencing of size-selected small subunit ribosomal RNA fragments. The library preparation protocol includes gel extraction of the target RNA, ligation of an RNA oligo to the 5'-end of the target, and cDNA synthesis with a tailed random-hexamer primer and further barcoding. The sequencing results of a phytoplankton mock community showed a highly similar profile to the biomass indicators. This method has universal potential for microbiome studies, and is compatible for the 5'-end sequencing of other RNA types with minimum library preparation costs.</p>', 'date' => '2018-10-05', 'pmid' => 'http://www.pubmed.gov/30284935', 'doi' => '10.2144/btn-2018-0082', 'modified' => '2018-12-31 11:24:16', 'created' => '2018-11-08 12:59:45', 'ProductsPublication' => array( 'id' => '2866', 'product_id' => '2844', 'publication_id' => '3401' ) ) $externalLink = ' <a href="http://www.pubmed.gov/30284935" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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