D-Plex mRNA Capture Module

Catalog Number

Format

Price

C05030032

24 rxns

$80.00

Diagenode’s D-Plex mRNA Capture Module is based on oligo d(T) magnetic beads to select only poly(A)-tailed RNAs such as mRNAs and some lncRNAs. This method allows manual processing of several samples at a time and can be also adapted for an automated platform.

The module is using 1 μm paramagnetic beads coated with oligo d(T)25 -mers for magnetic isolation through a gentle pull-down procedure of intact, full-length mRNAs. It is recommended to use good quality RNA (RIN≥7) as template for mRNA capture. The resulting captured-mRNA sample is recommended to be used in combination with the D-Plex Total RNA-seq Kit.

Product features
- D-Plex compatibility: Validated as an enrichment method for D-Plex RNA-seq library preparation protocols
- Save time. Designed to isolate in 1 hour intact poly(A)-tailed RNAs from cells and tissue
- Achieve highly effective purification: Generate mRNA sample that is intact and free of DNAs and other RNAs.

Related products
- C05030031
  
  D-Plex Total RNA-seq Kit for Illumina
- C05030033
  
  D-Plex mRNA-seq Kit for Illumina

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<li><b>Achieve highly effective purification:</b><span><span>&nbsp;</span>Generate mRNA sample that is intact and free of DNAs and other RNAs.</span></li>
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			'description' => '<div class="small-12 medium-12 large-12 columns" style="border: 3px solid #B02736; padding: 10px; margin: 10px;">
<h2>Product Features</h2>
<ul style="list-style-type: disc;">
<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
<li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li>
<li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li>
<li><span>Get high sensitivity data even from difficult samples, such as degraded, FFPE samples</span></li>
<li><span style="font-weight: 400;">Enjoy a fast, easy, single tube protocol</span></li>
</ul>
</div>
<p></p>
<p></p>
<h4></h4>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Total RNA-seq Library Preparation Kit is a tool&nbsp;designed for the study of the whole coding and non-coding transcriptome.&nbsp;<span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span>&nbsp;</span>D-Plex technology</a><span><span>&nbsp;</span>to generate&nbsp;directional&nbsp;libraries for Illumina sequencing directly from total RNAs, mRNAs&nbsp;that has already been enriched by poly(A) selection, or RNAs&nbsp;that has already been depleted of rRNAs.</span></p>
<p><span>The D-Plex technology utilizes&nbsp;two innovative&nbsp;ligation-free mechanisms - poly(A) tailing and template switching&nbsp;- to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs.&nbsp;Combined with unique molecular identifiers (UMI), this complete solution&nbsp;delivers&nbsp;a high-sensitivity detection&nbsp;method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p>
<p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time.&nbsp;<span>The library preparation takes place in a&nbsp;</span><span>single tube</span><span>, increasing the efficiency tremendously.&nbsp;This new solution has been extensively validated for&nbsp;both intact and highly degraded RNA&nbsp;samples, including that derived from FFPE preparations.</span></span></p>
<p><span><span>D-Plex&nbsp;Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C"><span>C05030023</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set C&nbsp;&nbsp;</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set D&nbsp;</a></li>
</ul>
<div class="extra-spaced" align="center"></div>
<div class="row">
<div class="carrousel" style="background-position: center;">
<div class="slick">
<div>
<h3>Greater sensitivity to detect novel transcripts</h3>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p>
</div>
</div>
<div>
<h3>Highest diversity to facilitate biomarker discovery</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p>
</div>
</div>
<div>
<h3>Consistent expression profiling across a wide range of RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p>
</div>
</div>
<div>
<h3>Superior library complexity at low RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p>
</div>
</div>
</div>
</div>
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<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C" title="D-Plex UDI Module - Set C" target="_blank"><span>C05030023</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set C</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set D&nbsp;</a></li>
</ul>
<p></p>
<p>The use of UDI is highly recommended&nbsp;to mitigate errors introduced by read misassignment, including index&nbsp;hopping frequently observed with patterned flow cells such as Illumina&rsquo;s&nbsp;NovaSeq system.</p>',
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<p><img src="https://www.diagenode.com/img/product/kits/dplex/bioinfoPipe.png" alt="Small RNA seq Bioinformatics pipeline" width="925" height="196" /></p>',
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			'description' => '<div class="small-12 medium-12 large-12 columns" style="border: 3px solid #B02736; padding: 10px; margin: 10px;">
<h2>Product Features</h2>
<ul style="list-style-type: disc;">
<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
<li><span>Ultra-sensitive methodology with low input capability down to 10 ng</span></li>
<li><span>High efficiency for greater gene detection and biomarker identification</span></li>
<li><span>Consistent gene expression profiling, optimal for gene signature definition</span></li>
<li><span>Precise measurement of strand orientation for anti-sense transcription detection</span></li>
</ul>
</div>
<h4></h4>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-mRNA-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>The D-Plex mRNA-seq Library Preparation Kit is a tool&nbsp;designed for gene expression analysis.&nbsp;<span>The kit utilizes the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span>&nbsp;</span>D-Plex technology</a><span><span>&nbsp;</span>to generate&nbsp;directional&nbsp;libraries for Illumina sequencing directly from total RNAs.</span></p>
<p><span>The D-Plex technology utilizes&nbsp;two innovative&nbsp;ligation-free mechanisms - poly(A) tailing and template switching&nbsp;- to produce&nbsp;sequencing libraries from low input samples, down to 10 ng of total RNAs.&nbsp;Combined with unique molecular identifiers (UMI), this complete solution&nbsp;delivers&nbsp;a high-sensitivity detection&nbsp;method for gene expression analysis and discovery applications in the coding transcriptome.</span></p>
<p><span></span><span>The D-Plex mRNA-seq Kit offers a time-saving protocol that can be completed within 6 hours and requires minimal hands-on time.&nbsp;<span>The library preparation takes place in a&nbsp;</span><span>single tube</span><span>, increasing the efficiency tremendously.&nbsp;This new solution has been developped to capture &nbsp;an increased number of genes for great discovery efficiency.</span></span></p>
<p><span><span>The D-Plex mRNA-seq Kit includes all buffers and enzymes necessary for mRNA capture and library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
</ul>
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<h3>Greater&nbsp;gene discovery efficiency at low inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/UHR_mRNA.png" alt="total RNA kit" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq kit enables the detection of higher numbers of genes even for low RNA inputs. Increased number of genes detected at 10x coverage is an indicator of greater sensitivity.</p>
</div>
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<div>
<h3>High mappability to the coding transcriptome</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_mRNA_biotypes.png" alt="total RNA diversity" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq libraries capture efficiently coding exons and introns present in the sample of interest.</p>
</div>
</div>
<div>
<h3>Consistent, sensitive gene expression profiling</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_mRNA_violin_tpm_10.png" alt="total RNA" height="200px" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>Gene expression profiling obtained with D-Plex mRNA-seq kit is conserved across a broad dynamic range of inputs, especially for the lowest RNA inputs.</p>
</div>
</div>
<div>
<h3>Exceptional data concordance at low inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_mrna.png" alt="total RNA" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq kit produces quality data with high data concordance between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for precious samples with limited starting material.</p>
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			'name' => '次世代シーケンシング',
			'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h2 style="font-size: 22px;">DNA断片化、ライブラリー調製、自動化：NGSのワンストップショップ</h2>
<table class="small-12 medium-12 large-12 columns">
<tbody>
<tr>
<th class="small-12 medium-12 large-12 columns">
<h4>1. 断片化装置を選択してください：150 bp〜75 kbの範囲でDNAを断片化します。</h4>
</th>
</tr>
<tr style="background-color: #ffffff;">
<td class="small-12 medium-12 large-12 columns"></td>
</tr>
<tr>
<td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-pico-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/bioruptor_pico.jpg" /></a></td>
<td class="small-4 medium-4 large-4 columns"><a href="../p/megaruptor2-1-unit"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010001_megaruptor2.jpg" /></a></td>
<td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-one-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-profil.png" /></a></td>
</tr>
<tr>
<td class="small-4 medium-4 large-4 columns">5&mu;lまで断片化：150 bp〜2 kb<br />NGS DNAライブラリー調製およびFFPE核酸抽出に最適で、</td>
<td class="small-4 medium-4 large-4 columns">2 kb〜75 kbの範囲をできます。<br />メイトペアライブラリー調製および長いフラグメントDNAシーケンシングに最適で、この軽量デスクトップデバイスで</td>
<td class="small-4 medium-4 large-4 columns">20または50&mu;lの断片化が可能です。</td>
</tr>
</tbody>
</table>
<table class="small-12 medium-12 large-12 columns">
<tbody>
<tr>
<th class="small-8 medium-8 large-8 columns">
<h4>2. 最適化されたライブラリー調整キットを選択してください。</h4>
</th>
<th class="small-4 medium-4 large-4 columns">
<h4>3. ライブラリー前処理自動化を選択して、比類のないデータ再現性を実感</h4>
</th>
</tr>
<tr style="background-color: #ffffff;">
<td class="small-12 medium-12 large-12 columns"></td>
</tr>
<tr>
<td class="small-4 medium-4 large-4 columns"><a href="../p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><img src="https://www.diagenode.com/img/product/kits/microPlex_library_preparation.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td>
<td class="small-4 medium-4 large-4 columns"><a href="../p/ideal-library-preparation-kit-x24-incl-index-primer-set-1-24-rxns"><img src="https://www.diagenode.com/img/product/kits/box_kit.jpg" style="display: block; margin-left: auto; margin-right: auto;" height="173" width="250" /></a></td>
<td class="small-4 medium-4 large-4 columns"><a href="../p/sx-8g-ip-star-compact-automated-system-1-unit"><img src="https://www.diagenode.com/img/product/automation/B03000002%20_ipstar_compact.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td>
</tr>
<tr>
<td class="small-4 medium-4 large-4 columns" style="text-align: center;">50pgの低入力：MicroPlex Library Preparation Kit</td>
<td class="small-4 medium-4 large-4 columns" style="text-align: center;">5ng以上：iDeal&nbsp;Library Preparation Kit</td>
<td class="small-4 medium-4 large-4 columns" style="text-align: center;">Achieve great NGS data easily</td>
</tr>
</tbody>
</table>
</div>
</div>
<blockquote>
<div class="row">
<div class="small-12 medium-12 large-12 columns"><span class="label" style="margin-bottom: 16px; margin-left: -22px; font-size: 15px;">DiagenodeがNGS研究にぴったりなプロバイダーである理由</span>
<p>Diagenodeは15年以上もエピジェネティクス研究に専念、専門としています。 ChIP研究クロマチン用のユニークな断片化システムの開発から始まり、 専門知識を活かし、5&mu;lのせん断体積まで可能で、NGS DNAライブラリーの調製に最適な最先端DNA断片化装置の開発にたどり着きました。 我々は以来、ChIP-seq、Methyl-seq、NGSライブラリー調製用キットを研究開発し、業界をリードする免疫沈降研究と同様に、ライブラリー調製を自動化および完結させる独自の自動化システムを開発にも成功しました。</p>
<ul>
<li>信頼されるせん断装置</li>
<li>様々なインプットからのライブラリ作成キット</li>
<li>独自の自動化デバイス</li>
</ul>
</div>
</div>
</blockquote>
<div class="row">
<div class="small-12 columns">
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#panel1a">次世代シーケンシングへの理解とその専門知識</a>
<div id="panel1a" class="content">
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>次世代シーケンシング (NGS)</strong> ）は、著しいスケールとハイスループットでシーケンシングを行い、1日に数十億もの塩基生成を可能にします。 NGSのハイスループットは迅速でありながら正確で、再現性のあるデータセットを実現し、さらにシーケンシング費用を削減します。 NGSは、ゲノムシーケンシング、ゲノム再シーケンシング、デノボシーケンシング、トランスクリプトームシーケンシング、その他にDNA-タンパク質相互作用の検出やエピゲノムなどを示します。 指数関数的に増加するシーケンシングデータの需要は、計算分析の障害や解釈、データストレージなどの課題を解決します。</p>
<p>アプリケーションおよび出発物質に応じて、数百万から数十億の鋳型DNA分子を大規模に並行してシーケンシングすることが可能です。その為に、異なる化学物質を使用するいくつかの市販のNGSプラットフォームを利用することができます。 NGSプラットフォームの種類によっては、事前準備とライブラリー作成が必要です。</p>
<p>NGSにとっても、特にデータ処理と分析に関した大きな課題はあります。第3世代技術はゲノミクス研究にさらに革命を起こすであろうと大きく期待されています。</p>
</div>
</div>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<p><strong>NGS アプリケーション</strong></p>
<ul>
<li>全ゲノム配列決定</li>
<li>デノボシーケンシング</li>
<li>標的配列</li>
<li>Exomeシーケンシング</li>
<li>トランスクリプトーム配列決定</li>
<li>ゲノム配列決定</li>
<li>ミトコンドリア配列決定</li>
<li>DNA-タンパク質相互作用（ChIP-seq</li>
<li>バリアント検出</li>
<li>ゲノム仕上げ</li>
</ul>
</div>
<div class="small-6 medium-6 large-6 columns">
<p><strong>研究分野におけるNGS:</strong></p>
<ul>
<li>腫瘍学</li>
<li>リプロダクティブ・ヘルス</li>
<li>法医学ゲノミクス</li>
<li>アグリゲノミックス</li>
<li>複雑な病気</li>
<li>微生物ゲノミクス</li>
<li>食品・環境ゲノミクス</li>
<li>創薬ゲノミクス - パーソナライズド・メディカル</li>
</ul>
</div>
<div class="small-12 medium-12 large-12 columns">
<p><strong>NGSの用語</strong></p>
<dl>
<dt>リード（読み取り）</dt>
<dd>この装置から得られた連続した単一のストレッチ</dd>
<dt>断片リード</dt>
<dd>フラグメントライブラリからの読み込み。 シーケンシングプラットフォームに応じて、読み取りは通常約100〜300bp。</dd>
<dt>断片ペアエンドリード</dt>
<dd>断片ライブラリーからDNA断片の各末端2つの読み取り。</dd>
<dt>メイトペアリード</dt>
<dd>大きなDNA断片（通常は予め定義されたサイズ範囲）の各末端から2つの読み取り。</dd>
<dt>カバレッジ（例）</dt>
<dd>30&times;適用範囲とは、参照ゲノム中の各塩基対が平均30回の読み取りを示す。</dd>
</dl>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h2>NGSプラットフォーム</h2>
<h3><a href="http://www.illumina.com" target="_blank">イルミナ</a></h3>
<p>イルミナは、クローン的に増幅された鋳型DNA（クラスター）上に位置する、蛍光標識された可逆的鎖ターミネーターヌクレオチドを用いた配列別合成技術を使用。 DNAクラスターは、ガラスフローセルの表面上に固定化され、 ワークフローは、4つのヌクレオチド（それぞれ異なる蛍光色素で標識された）の組み込み、4色イメージング、色素や末端基の切断、取り込み、イメージングなどを繰り返します。フローセルは大規模な並列配列決定を受ける。 この方法により、単一蛍光標識されたヌクレオチドの制御添加によるモノヌクレオチドのエラーを回避する可能性があります。 読み取りの長さは、通常約100〜150 bpです。</p>
<h3><a href="http://www.lifetechnologies.com" target="_blank">イオン トレント</a></h3>
<p>イオントレントは、半導体技術チップを用いて、合成中にヌクレオチドを取り込む際に放出されたプロトンを検出します。 これは、イオン球粒子と呼ばれるビーズの表面にエマルションPCR（emPCR）を使用し、リンクされた特定のアダプターを用いてDNA断片を増幅します。 各ビーズは1種類のDNA断片で覆われていて、異なるDNA断片を有するビーズは次いで、チップの陽子感知ウェル内に配置されます。 チップには一度に4つのヌクレオチドのうちの1つが浸水し、このプロセスは異なるヌクレオチドで15秒ごとに繰り返されます。 配列決定の間に4つの塩基の各々が1つずつ導入されます、組み込みの場合はプロトンが放出され、電圧信号が取り込みに比例して検出されます。.</p>
<h3><a href="http://www.pacificbiosciences.com" target="_blank">パシフィック バイオサイエンス</a></h3>
<p>パシフィックバイオサイエンスでは、20kbを超える塩基対の読み取りも、単一分子リアルタイム（SMRT）シーケンシングによる構造および細胞タイプの変化を観察することができます。 このプラットフォームでは、超長鎖二本鎖DNA（dsDNA）断片が、Megaruptor（登録商標）のようなDiagenode装置を用いたランダムシアリングまたは目的の標的領域の増幅によって生成されます。 SMRTbellライブラリーは、ユニバーサルヘアピンアダプターをDNA断片の各末端に連結することによって生成します。 サイズ選択条件による洗浄ステップの後、配列決定プライマーをSMRTbellテンプレートにアニーリングし、鋳型DNAに結合したDNAポリメラーゼを含む配列決定を、蛍光標識ヌクレオチドの存在下で開始。 各塩基が取り込まれると、異なる蛍光のパルスをリアルタイムで検出します。</p>
<h3><a href="https://nanoporetech.com" target="_blank">オックスフォード ナノポア</a></h3>
<p>Oxford Nanoporeは、単一のDNA分子配列決定に基づく技術を開発します。その技術により生物学的分子、すなわちDNAが一群の電気抵抗性高分子膜として位置するナノスケールの孔（ナノ細孔）またはその近くを通過し、イオン電流が変化します。 この変化に関する情報は、例えば4つのヌクレオチド（AまたはG r CまたはT）ならびに修飾されたヌクレオチドすべてを区別することによって分子情報に訳されます。 シーケンシングミニオンデバイスのフローセルは、数百個のナノポアチャネルのセンサアレイを含みます。 DNAサンプルは、Diagenode社のMegaruptor（登録商標）を用いてランダムシアリングによって生成され得る超長鎖DNAフラグメントが必要です。</p>
<h3><a href="http://www.lifetechnologies.com/be/en/home/life-science/sequencing/next-generation-sequencing/solid-next-generation-sequencing.html" target="_blank">SOLiD</a></h3>
<p>SOLiDは、ユニークな化学作用により、何千という個々のDNA分子の同時配列決定を可能にします。 それは、アダプター対ライブラリーのフラグメントが適切で、せん断されたゲノムDNAへのアダプターのライゲーションによるライブラリー作製から始まります。 次のステップでは、エマルジョンPCR（emPCR）を実施して、ビーズの表面上の個々の鋳型DNA分子をクローン的に増幅。 emPCRでは、個々の鋳型DNAをPCR試薬と混合し、水中油型エマルジョン内の疎水性シェルで囲まれた水性液滴内のプライマーコートビーズを、配列決定のためにロードするスライドガラスの表面にランダムに付着。 この技術は、シークエンシングプライマーへのライゲーションで競合する4つの蛍光標識されたジ塩基プローブのセットを使用します。</p>
<h3><a href="http://454.com/products/technology.asp" target="_blank">454</a></h3>
<p>454は、大規模並列パイロシーケンシングを利用しています。 始めに全ゲノムDNAまたは標的遺伝子断片の300〜800bp断片のライブラリー調製します。 次に、DNAフラグメントへのアダプターの付着および単一のDNA鎖の分離。 その後アダプターに連結されたDNAフラグメントをエマルジョンベースのクローン増幅（emPCR）で処理し、DNAライブラリーフラグメントをミクロンサイズのビーズ上に配置します。 各DNA結合ビーズを光ファイバーチップ上のウェルに入れ、器具に挿入します。 4つのDNAヌクレオチドは、配列決定操作中に固定された順序で連続して加えられ、並行して配列決定されます。</p>
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<p><span style="font-weight: 400;">Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to </span><a href="../applications/dna-rna-shearing"><span style="font-weight: 400;">fragmented DNA or RNA</span></a><span style="font-weight: 400;"> prior to sequencing</span></p>
<p><span style="font-weight: 400;">After input DNA has been fragmented, it is end-repaired and blunt-ended</span><span style="font-weight: 400;">. The next step is a A-tailing in which dAMP is added to the 3&acute; end of the blunt phosphorylated DNA fragments to prevent concatemerization and to allow the ligation of adaptors with complementary dT overhangs. In addition, barcoded adapters can be incorporated to facilitate multiplexing prior to or during amplification.</span></p>
<center><img src="https://www.diagenode.com/img/categories/library-prep/flux.png" /></center>
<p><span style="font-weight: 400;">Diagenode offers a comprehensive product portfolio for library preparation:<br /></span></p>
<strong><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex&nbsp;RNA-seq Library Preparation Kits</a></strong><br />
<p><span style="font-weight: 400;">Diagenode&rsquo;s new RNA-sequencing solutions utilize the innovative c</span><span style="font-weight: 400;">apture and a</span><span style="font-weight: 400;">mplification by t</span><span style="font-weight: 400;">ailing and s</span><span style="font-weight: 400;">witching&rdquo;</span><span style="font-weight: 400;">, a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of RNA. </span><span style="font-weight: 400;"></span><a href="../categories/Library-preparation-for-RNA-seq">Learn more</a></p>
<strong><a href="../categories/library-preparation-for-ChIP-seq">ChIP-seq and DNA sequencing library preparation solutions</a></strong><br />
<p><span style="font-weight: 400;">Our kits have been optimized for DNA library preparation used for next generation sequencing for a wide range of inputs. Using a simple three-step protocols, our</span><a href="http://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><span style="font-weight: 400;"> </span></a><span style="font-weight: 400;">kits are an optimal choice for library preparation from DNA inputs down to 50 pg.&nbsp;</span><a href="../categories/library-preparation-for-ChIP-seq">Learn more</a></p>
<a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span><strong>Bioruptor Pico - short fragments</strong></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br />
<p><span style="font-weight: 400;"></span><span style="font-weight: 400;">Our well-cited Bioruptor Pico is the shearing device of choice for chromatin and DNA fragmentation. Obtain uniform and tight fragment distributions &nbsp;between 150bp -2kb. </span><a href="../p/bioruptor-pico-sonication-device">Learn more</a></p>
<strong><a href="../p/megaruptor2-1-unit"><span href="../p/bioruptor-pico-sonication-device">Megaruptor</span>&reg; - long fragments</a></strong><a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br />
<p><span style="font-weight: 400;"></span><span style="font-weight: 400;">The Megaruptor is designed to shear DNA from 3kb-75kb for long-read sequencing.&nbsp; <a href="../p/megaruptor2-1-unit">Learn more</a></span></p>
<span href="../p/bioruptor-pico-sonication-device"></span><span style="font-weight: 400;"></span></div>
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<h2>Product Features</h2>
<ul style="list-style-type: disc;">
<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
<li><span>Ultra-sensitive methodology with low input capability down to 10 ng</span></li>
<li><span>High efficiency for greater gene detection and biomarker identification</span></li>
<li><span>Consistent gene expression profiling, optimal for gene signature definition</span></li>
<li><span>Precise measurement of strand orientation for anti-sense transcription detection</span></li>
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<h4></h4>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-mRNA-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>The D-Plex mRNA-seq Library Preparation Kit is a tool&nbsp;designed for gene expression analysis.&nbsp;<span>The kit utilizes the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span>&nbsp;</span>D-Plex technology</a><span><span>&nbsp;</span>to generate&nbsp;directional&nbsp;libraries for Illumina sequencing directly from total RNAs.</span></p>
<p><span>The D-Plex technology utilizes&nbsp;two innovative&nbsp;ligation-free mechanisms - poly(A) tailing and template switching&nbsp;- to produce&nbsp;sequencing libraries from low input samples, down to 10 ng of total RNAs.&nbsp;Combined with unique molecular identifiers (UMI), this complete solution&nbsp;delivers&nbsp;a high-sensitivity detection&nbsp;method for gene expression analysis and discovery applications in the coding transcriptome.</span></p>
<p><span></span><span>The D-Plex mRNA-seq Kit offers a time-saving protocol that can be completed within 6 hours and requires minimal hands-on time.&nbsp;<span>The library preparation takes place in a&nbsp;</span><span>single tube</span><span>, increasing the efficiency tremendously.&nbsp;This new solution has been developped to capture &nbsp;an increased number of genes for great discovery efficiency.</span></span></p>
<p><span><span>The D-Plex mRNA-seq Kit includes all buffers and enzymes necessary for mRNA capture and library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
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<h3>Greater&nbsp;gene discovery efficiency at low inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/UHR_mRNA.png" alt="total RNA kit" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq kit enables the detection of higher numbers of genes even for low RNA inputs. Increased number of genes detected at 10x coverage is an indicator of greater sensitivity.</p>
</div>
</div>
<div>
<h3>High mappability to the coding transcriptome</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_mRNA_biotypes.png" alt="total RNA diversity" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq libraries capture efficiently coding exons and introns present in the sample of interest.</p>
</div>
</div>
<div>
<h3>Consistent, sensitive gene expression profiling</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_mRNA_violin_tpm_10.png" alt="total RNA" height="200px" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>Gene expression profiling obtained with D-Plex mRNA-seq kit is conserved across a broad dynamic range of inputs, especially for the lowest RNA inputs.</p>
</div>
</div>
<div>
<h3>Exceptional data concordance at low inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_mrna.png" alt="total RNA" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq kit produces quality data with high data concordance between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for precious samples with limited starting material.</p>
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<p><span>The use of UDI is highly recommended&nbsp;to mitigate errors introduced by read misassignment, including index&nbsp;hopping frequently observed with patterned flow cells such as Illumina&rsquo;s&nbsp;NovaSeq system.</span></p>
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			'description' => '<div class="small-12 medium-12 large-12 columns" style="border: 3px solid #B02736; padding: 10px; margin: 10px;">
<h2>Product Features</h2>
<ul style="list-style-type: disc;">
<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
<li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li>
<li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li>
<li><span>Get high sensitivity data even from difficult samples, such as degraded, FFPE samples</span></li>
<li><span style="font-weight: 400;">Enjoy a fast, easy, single tube protocol</span></li>
</ul>
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<p></p>
<p></p>
<h4></h4>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Total RNA-seq Library Preparation Kit is a tool&nbsp;designed for the study of the whole coding and non-coding transcriptome.&nbsp;<span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span>&nbsp;</span>D-Plex technology</a><span><span>&nbsp;</span>to generate&nbsp;directional&nbsp;libraries for Illumina sequencing directly from total RNAs, mRNAs&nbsp;that has already been enriched by poly(A) selection, or RNAs&nbsp;that has already been depleted of rRNAs.</span></p>
<p><span>The D-Plex technology utilizes&nbsp;two innovative&nbsp;ligation-free mechanisms - poly(A) tailing and template switching&nbsp;- to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs.&nbsp;Combined with unique molecular identifiers (UMI), this complete solution&nbsp;delivers&nbsp;a high-sensitivity detection&nbsp;method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p>
<p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time.&nbsp;<span>The library preparation takes place in a&nbsp;</span><span>single tube</span><span>, increasing the efficiency tremendously.&nbsp;This new solution has been extensively validated for&nbsp;both intact and highly degraded RNA&nbsp;samples, including that derived from FFPE preparations.</span></span></p>
<p><span><span>D-Plex&nbsp;Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C"><span>C05030023</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set C&nbsp;&nbsp;</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set D&nbsp;</a></li>
</ul>
<div class="extra-spaced" align="center"></div>
<div class="row">
<div class="carrousel" style="background-position: center;">
<div class="slick">
<div>
<h3>Greater sensitivity to detect novel transcripts</h3>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p>
</div>
</div>
<div>
<h3>Highest diversity to facilitate biomarker discovery</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p>
</div>
</div>
<div>
<h3>Consistent expression profiling across a wide range of RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p>
</div>
</div>
<div>
<h3>Superior library complexity at low RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p>
</div>
</div>
</div>
</div>
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<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C" title="D-Plex UDI Module - Set C" target="_blank"><span>C05030023</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set C</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set D&nbsp;</a></li>
</ul>
<p></p>
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			'meta_title' => 'D-Plex Total RNA-seq Library Prep Kit for Illumina | Diagenode ',
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			'description' => '<div class="small-12 medium-12 large-12 columns" style="border: 3px solid #B02736; padding: 10px; margin: 10px;">
<h2>Product Features</h2>
<ul style="list-style-type: disc;">
<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
<li><span>Ultra-sensitive methodology with low input capability down to 10 ng</span></li>
<li><span>High efficiency for greater gene detection and biomarker identification</span></li>
<li><span>Consistent gene expression profiling, optimal for gene signature definition</span></li>
<li><span>Precise measurement of strand orientation for anti-sense transcription detection</span></li>
</ul>
</div>
<h4></h4>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-mRNA-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>The D-Plex mRNA-seq Library Preparation Kit is a tool&nbsp;designed for gene expression analysis.&nbsp;<span>The kit utilizes the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span>&nbsp;</span>D-Plex technology</a><span><span>&nbsp;</span>to generate&nbsp;directional&nbsp;libraries for Illumina sequencing directly from total RNAs.</span></p>
<p><span>The D-Plex technology utilizes&nbsp;two innovative&nbsp;ligation-free mechanisms - poly(A) tailing and template switching&nbsp;- to produce&nbsp;sequencing libraries from low input samples, down to 10 ng of total RNAs.&nbsp;Combined with unique molecular identifiers (UMI), this complete solution&nbsp;delivers&nbsp;a high-sensitivity detection&nbsp;method for gene expression analysis and discovery applications in the coding transcriptome.</span></p>
<p><span></span><span>The D-Plex mRNA-seq Kit offers a time-saving protocol that can be completed within 6 hours and requires minimal hands-on time.&nbsp;<span>The library preparation takes place in a&nbsp;</span><span>single tube</span><span>, increasing the efficiency tremendously.&nbsp;This new solution has been developped to capture &nbsp;an increased number of genes for great discovery efficiency.</span></span></p>
<p><span><span>The D-Plex mRNA-seq Kit includes all buffers and enzymes necessary for mRNA capture and library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
</ul>
<div class="extra-spaced" align="center"></div>
<div class="row">
<div class="carrousel" style="background-position: center;">
<div class="slick">
<div>
<h3>Greater&nbsp;gene discovery efficiency at low inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/UHR_mRNA.png" alt="total RNA kit" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq kit enables the detection of higher numbers of genes even for low RNA inputs. Increased number of genes detected at 10x coverage is an indicator of greater sensitivity.</p>
</div>
</div>
<div>
<h3>High mappability to the coding transcriptome</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_mRNA_biotypes.png" alt="total RNA diversity" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq libraries capture efficiently coding exons and introns present in the sample of interest.</p>
</div>
</div>
<div>
<h3>Consistent, sensitive gene expression profiling</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_mRNA_violin_tpm_10.png" alt="total RNA" height="200px" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>Gene expression profiling obtained with D-Plex mRNA-seq kit is conserved across a broad dynamic range of inputs, especially for the lowest RNA inputs.</p>
</div>
</div>
<div>
<h3>Exceptional data concordance at low inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_mrna.png" alt="total RNA" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq kit produces quality data with high data concordance between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for precious samples with limited starting material.</p>
</div>
</div>
</div>
</div>
</div>
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			'info2' => '<p><span>Specific D-Plex indexes&nbsp;</span><span>were designed and validated to fit the D-Plex technology for Illumina sequencing&nbsp;and&nbsp;</span><span>are not included in the kit. They can be bought separately according to your needs. Please choose&nbsp;the format that suits you best among the compatible references to:</span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
</ul>
<p><span></span></p>
<p><span>The use of UDI is highly recommended&nbsp;to mitigate errors introduced by read misassignment, including index&nbsp;hopping frequently observed with patterned flow cells such as Illumina&rsquo;s&nbsp;NovaSeq system.</span></p>
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<p><img src="https://www.diagenode.com/img/product/kits/dplex/bioinfoPipe.png" alt="small RNA sequencing bioinformatics pipeline" caption="false" width="925" height="196" /></p>
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			'name' => '次世代シーケンシング',
			'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h2 style="font-size: 22px;">DNA断片化、ライブラリー調製、自動化：NGSのワンストップショップ</h2>
<table class="small-12 medium-12 large-12 columns">
<tbody>
<tr>
<th class="small-12 medium-12 large-12 columns">
<h4>1. 断片化装置を選択してください：150 bp〜75 kbの範囲でDNAを断片化します。</h4>
</th>
</tr>
<tr style="background-color: #ffffff;">
<td class="small-12 medium-12 large-12 columns"></td>
</tr>
<tr>
<td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-pico-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/bioruptor_pico.jpg" /></a></td>
<td class="small-4 medium-4 large-4 columns"><a href="../p/megaruptor2-1-unit"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010001_megaruptor2.jpg" /></a></td>
<td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-one-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-profil.png" /></a></td>
</tr>
<tr>
<td class="small-4 medium-4 large-4 columns">5&mu;lまで断片化：150 bp〜2 kb<br />NGS DNAライブラリー調製およびFFPE核酸抽出に最適で、</td>
<td class="small-4 medium-4 large-4 columns">2 kb〜75 kbの範囲をできます。<br />メイトペアライブラリー調製および長いフラグメントDNAシーケンシングに最適で、この軽量デスクトップデバイスで</td>
<td class="small-4 medium-4 large-4 columns">20または50&mu;lの断片化が可能です。</td>
</tr>
</tbody>
</table>
<table class="small-12 medium-12 large-12 columns">
<tbody>
<tr>
<th class="small-8 medium-8 large-8 columns">
<h4>2. 最適化されたライブラリー調整キットを選択してください。</h4>
</th>
<th class="small-4 medium-4 large-4 columns">
<h4>3. ライブラリー前処理自動化を選択して、比類のないデータ再現性を実感</h4>
</th>
</tr>
<tr style="background-color: #ffffff;">
<td class="small-12 medium-12 large-12 columns"></td>
</tr>
<tr>
<td class="small-4 medium-4 large-4 columns"><a href="../p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><img src="https://www.diagenode.com/img/product/kits/microPlex_library_preparation.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td>
<td class="small-4 medium-4 large-4 columns"><a href="../p/ideal-library-preparation-kit-x24-incl-index-primer-set-1-24-rxns"><img src="https://www.diagenode.com/img/product/kits/box_kit.jpg" style="display: block; margin-left: auto; margin-right: auto;" height="173" width="250" /></a></td>
<td class="small-4 medium-4 large-4 columns"><a href="../p/sx-8g-ip-star-compact-automated-system-1-unit"><img src="https://www.diagenode.com/img/product/automation/B03000002%20_ipstar_compact.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td>
</tr>
<tr>
<td class="small-4 medium-4 large-4 columns" style="text-align: center;">50pgの低入力：MicroPlex Library Preparation Kit</td>
<td class="small-4 medium-4 large-4 columns" style="text-align: center;">5ng以上：iDeal&nbsp;Library Preparation Kit</td>
<td class="small-4 medium-4 large-4 columns" style="text-align: center;">Achieve great NGS data easily</td>
</tr>
</tbody>
</table>
</div>
</div>
<blockquote>
<div class="row">
<div class="small-12 medium-12 large-12 columns"><span class="label" style="margin-bottom: 16px; margin-left: -22px; font-size: 15px;">DiagenodeがNGS研究にぴったりなプロバイダーである理由</span>
<p>Diagenodeは15年以上もエピジェネティクス研究に専念、専門としています。 ChIP研究クロマチン用のユニークな断片化システムの開発から始まり、 専門知識を活かし、5&mu;lのせん断体積まで可能で、NGS DNAライブラリーの調製に最適な最先端DNA断片化装置の開発にたどり着きました。 我々は以来、ChIP-seq、Methyl-seq、NGSライブラリー調製用キットを研究開発し、業界をリードする免疫沈降研究と同様に、ライブラリー調製を自動化および完結させる独自の自動化システムを開発にも成功しました。</p>
<ul>
<li>信頼されるせん断装置</li>
<li>様々なインプットからのライブラリ作成キット</li>
<li>独自の自動化デバイス</li>
</ul>
</div>
</div>
</blockquote>
<div class="row">
<div class="small-12 columns">
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#panel1a">次世代シーケンシングへの理解とその専門知識</a>
<div id="panel1a" class="content">
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>次世代シーケンシング (NGS)</strong> ）は、著しいスケールとハイスループットでシーケンシングを行い、1日に数十億もの塩基生成を可能にします。 NGSのハイスループットは迅速でありながら正確で、再現性のあるデータセットを実現し、さらにシーケンシング費用を削減します。 NGSは、ゲノムシーケンシング、ゲノム再シーケンシング、デノボシーケンシング、トランスクリプトームシーケンシング、その他にDNA-タンパク質相互作用の検出やエピゲノムなどを示します。 指数関数的に増加するシーケンシングデータの需要は、計算分析の障害や解釈、データストレージなどの課題を解決します。</p>
<p>アプリケーションおよび出発物質に応じて、数百万から数十億の鋳型DNA分子を大規模に並行してシーケンシングすることが可能です。その為に、異なる化学物質を使用するいくつかの市販のNGSプラットフォームを利用することができます。 NGSプラットフォームの種類によっては、事前準備とライブラリー作成が必要です。</p>
<p>NGSにとっても、特にデータ処理と分析に関した大きな課題はあります。第3世代技術はゲノミクス研究にさらに革命を起こすであろうと大きく期待されています。</p>
</div>
</div>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<p><strong>NGS アプリケーション</strong></p>
<ul>
<li>全ゲノム配列決定</li>
<li>デノボシーケンシング</li>
<li>標的配列</li>
<li>Exomeシーケンシング</li>
<li>トランスクリプトーム配列決定</li>
<li>ゲノム配列決定</li>
<li>ミトコンドリア配列決定</li>
<li>DNA-タンパク質相互作用（ChIP-seq</li>
<li>バリアント検出</li>
<li>ゲノム仕上げ</li>
</ul>
</div>
<div class="small-6 medium-6 large-6 columns">
<p><strong>研究分野におけるNGS:</strong></p>
<ul>
<li>腫瘍学</li>
<li>リプロダクティブ・ヘルス</li>
<li>法医学ゲノミクス</li>
<li>アグリゲノミックス</li>
<li>複雑な病気</li>
<li>微生物ゲノミクス</li>
<li>食品・環境ゲノミクス</li>
<li>創薬ゲノミクス - パーソナライズド・メディカル</li>
</ul>
</div>
<div class="small-12 medium-12 large-12 columns">
<p><strong>NGSの用語</strong></p>
<dl>
<dt>リード（読み取り）</dt>
<dd>この装置から得られた連続した単一のストレッチ</dd>
<dt>断片リード</dt>
<dd>フラグメントライブラリからの読み込み。 シーケンシングプラットフォームに応じて、読み取りは通常約100〜300bp。</dd>
<dt>断片ペアエンドリード</dt>
<dd>断片ライブラリーからDNA断片の各末端2つの読み取り。</dd>
<dt>メイトペアリード</dt>
<dd>大きなDNA断片（通常は予め定義されたサイズ範囲）の各末端から2つの読み取り。</dd>
<dt>カバレッジ（例）</dt>
<dd>30&times;適用範囲とは、参照ゲノム中の各塩基対が平均30回の読み取りを示す。</dd>
</dl>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h2>NGSプラットフォーム</h2>
<h3><a href="http://www.illumina.com" target="_blank">イルミナ</a></h3>
<p>イルミナは、クローン的に増幅された鋳型DNA（クラスター）上に位置する、蛍光標識された可逆的鎖ターミネーターヌクレオチドを用いた配列別合成技術を使用。 DNAクラスターは、ガラスフローセルの表面上に固定化され、 ワークフローは、4つのヌクレオチド（それぞれ異なる蛍光色素で標識された）の組み込み、4色イメージング、色素や末端基の切断、取り込み、イメージングなどを繰り返します。フローセルは大規模な並列配列決定を受ける。 この方法により、単一蛍光標識されたヌクレオチドの制御添加によるモノヌクレオチドのエラーを回避する可能性があります。 読み取りの長さは、通常約100〜150 bpです。</p>
<h3><a href="http://www.lifetechnologies.com" target="_blank">イオン トレント</a></h3>
<p>イオントレントは、半導体技術チップを用いて、合成中にヌクレオチドを取り込む際に放出されたプロトンを検出します。 これは、イオン球粒子と呼ばれるビーズの表面にエマルションPCR（emPCR）を使用し、リンクされた特定のアダプターを用いてDNA断片を増幅します。 各ビーズは1種類のDNA断片で覆われていて、異なるDNA断片を有するビーズは次いで、チップの陽子感知ウェル内に配置されます。 チップには一度に4つのヌクレオチドのうちの1つが浸水し、このプロセスは異なるヌクレオチドで15秒ごとに繰り返されます。 配列決定の間に4つの塩基の各々が1つずつ導入されます、組み込みの場合はプロトンが放出され、電圧信号が取り込みに比例して検出されます。.</p>
<h3><a href="http://www.pacificbiosciences.com" target="_blank">パシフィック バイオサイエンス</a></h3>
<p>パシフィックバイオサイエンスでは、20kbを超える塩基対の読み取りも、単一分子リアルタイム（SMRT）シーケンシングによる構造および細胞タイプの変化を観察することができます。 このプラットフォームでは、超長鎖二本鎖DNA（dsDNA）断片が、Megaruptor（登録商標）のようなDiagenode装置を用いたランダムシアリングまたは目的の標的領域の増幅によって生成されます。 SMRTbellライブラリーは、ユニバーサルヘアピンアダプターをDNA断片の各末端に連結することによって生成します。 サイズ選択条件による洗浄ステップの後、配列決定プライマーをSMRTbellテンプレートにアニーリングし、鋳型DNAに結合したDNAポリメラーゼを含む配列決定を、蛍光標識ヌクレオチドの存在下で開始。 各塩基が取り込まれると、異なる蛍光のパルスをリアルタイムで検出します。</p>
<h3><a href="https://nanoporetech.com" target="_blank">オックスフォード ナノポア</a></h3>
<p>Oxford Nanoporeは、単一のDNA分子配列決定に基づく技術を開発します。その技術により生物学的分子、すなわちDNAが一群の電気抵抗性高分子膜として位置するナノスケールの孔（ナノ細孔）またはその近くを通過し、イオン電流が変化します。 この変化に関する情報は、例えば4つのヌクレオチド（AまたはG r CまたはT）ならびに修飾されたヌクレオチドすべてを区別することによって分子情報に訳されます。 シーケンシングミニオンデバイスのフローセルは、数百個のナノポアチャネルのセンサアレイを含みます。 DNAサンプルは、Diagenode社のMegaruptor（登録商標）を用いてランダムシアリングによって生成され得る超長鎖DNAフラグメントが必要です。</p>
<h3><a href="http://www.lifetechnologies.com/be/en/home/life-science/sequencing/next-generation-sequencing/solid-next-generation-sequencing.html" target="_blank">SOLiD</a></h3>
<p>SOLiDは、ユニークな化学作用により、何千という個々のDNA分子の同時配列決定を可能にします。 それは、アダプター対ライブラリーのフラグメントが適切で、せん断されたゲノムDNAへのアダプターのライゲーションによるライブラリー作製から始まります。 次のステップでは、エマルジョンPCR（emPCR）を実施して、ビーズの表面上の個々の鋳型DNA分子をクローン的に増幅。 emPCRでは、個々の鋳型DNAをPCR試薬と混合し、水中油型エマルジョン内の疎水性シェルで囲まれた水性液滴内のプライマーコートビーズを、配列決定のためにロードするスライドガラスの表面にランダムに付着。 この技術は、シークエンシングプライマーへのライゲーションで競合する4つの蛍光標識されたジ塩基プローブのセットを使用します。</p>
<h3><a href="http://454.com/products/technology.asp" target="_blank">454</a></h3>
<p>454は、大規模並列パイロシーケンシングを利用しています。 始めに全ゲノムDNAまたは標的遺伝子断片の300〜800bp断片のライブラリー調製します。 次に、DNAフラグメントへのアダプターの付着および単一のDNA鎖の分離。 その後アダプターに連結されたDNAフラグメントをエマルジョンベースのクローン増幅（emPCR）で処理し、DNAライブラリーフラグメントをミクロンサイズのビーズ上に配置します。 各DNA結合ビーズを光ファイバーチップ上のウェルに入れ、器具に挿入します。 4つのDNAヌクレオチドは、配列決定操作中に固定された順序で連続して加えられ、並行して配列決定されます。</p>
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<p><span style="font-weight: 400;">Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to </span><a href="../applications/dna-rna-shearing"><span style="font-weight: 400;">fragmented DNA or RNA</span></a><span style="font-weight: 400;"> prior to sequencing</span></p>
<p><span style="font-weight: 400;">After input DNA has been fragmented, it is end-repaired and blunt-ended</span><span style="font-weight: 400;">. The next step is a A-tailing in which dAMP is added to the 3&acute; end of the blunt phosphorylated DNA fragments to prevent concatemerization and to allow the ligation of adaptors with complementary dT overhangs. In addition, barcoded adapters can be incorporated to facilitate multiplexing prior to or during amplification.</span></p>
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<p><span style="font-weight: 400;">Diagenode offers a comprehensive product portfolio for library preparation:<br /></span></p>
<strong><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex&nbsp;RNA-seq Library Preparation Kits</a></strong><br />
<p><span style="font-weight: 400;">Diagenode&rsquo;s new RNA-sequencing solutions utilize the innovative c</span><span style="font-weight: 400;">apture and a</span><span style="font-weight: 400;">mplification by t</span><span style="font-weight: 400;">ailing and s</span><span style="font-weight: 400;">witching&rdquo;</span><span style="font-weight: 400;">, a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of RNA. </span><span style="font-weight: 400;"></span><a href="../categories/Library-preparation-for-RNA-seq">Learn more</a></p>
<strong><a href="../categories/library-preparation-for-ChIP-seq">ChIP-seq and DNA sequencing library preparation solutions</a></strong><br />
<p><span style="font-weight: 400;">Our kits have been optimized for DNA library preparation used for next generation sequencing for a wide range of inputs. Using a simple three-step protocols, our</span><a href="http://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><span style="font-weight: 400;"> </span></a><span style="font-weight: 400;">kits are an optimal choice for library preparation from DNA inputs down to 50 pg.&nbsp;</span><a href="../categories/library-preparation-for-ChIP-seq">Learn more</a></p>
<a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span><strong>Bioruptor Pico - short fragments</strong></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br />
<p><span style="font-weight: 400;"></span><span style="font-weight: 400;">Our well-cited Bioruptor Pico is the shearing device of choice for chromatin and DNA fragmentation. Obtain uniform and tight fragment distributions &nbsp;between 150bp -2kb. </span><a href="../p/bioruptor-pico-sonication-device">Learn more</a></p>
<strong><a href="../p/megaruptor2-1-unit"><span href="../p/bioruptor-pico-sonication-device">Megaruptor</span>&reg; - long fragments</a></strong><a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br />
<p><span style="font-weight: 400;"></span><span style="font-weight: 400;">The Megaruptor is designed to shear DNA from 3kb-75kb for long-read sequencing.&nbsp; <a href="../p/megaruptor2-1-unit">Learn more</a></span></p>
<span href="../p/bioruptor-pico-sonication-device"></span><span style="font-weight: 400;"></span></div>
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        <div class="small-12 columns" >
            <h6 style="height:60px">D-Plex mRNA-seq Kit for Illumina</h6>
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<h2>Product Features</h2>
<ul style="list-style-type: disc;">
<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
<li><span>Ultra-sensitive methodology with low input capability down to 10 ng</span></li>
<li><span>High efficiency for greater gene detection and biomarker identification</span></li>
<li><span>Consistent gene expression profiling, optimal for gene signature definition</span></li>
<li><span>Precise measurement of strand orientation for anti-sense transcription detection</span></li>
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<h4></h4>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-mRNA-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>The D-Plex mRNA-seq Library Preparation Kit is a tool&nbsp;designed for gene expression analysis.&nbsp;<span>The kit utilizes the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span>&nbsp;</span>D-Plex technology</a><span><span>&nbsp;</span>to generate&nbsp;directional&nbsp;libraries for Illumina sequencing directly from total RNAs.</span></p>
<p><span>The D-Plex technology utilizes&nbsp;two innovative&nbsp;ligation-free mechanisms - poly(A) tailing and template switching&nbsp;- to produce&nbsp;sequencing libraries from low input samples, down to 10 ng of total RNAs.&nbsp;Combined with unique molecular identifiers (UMI), this complete solution&nbsp;delivers&nbsp;a high-sensitivity detection&nbsp;method for gene expression analysis and discovery applications in the coding transcriptome.</span></p>
<p><span></span><span>The D-Plex mRNA-seq Kit offers a time-saving protocol that can be completed within 6 hours and requires minimal hands-on time.&nbsp;<span>The library preparation takes place in a&nbsp;</span><span>single tube</span><span>, increasing the efficiency tremendously.&nbsp;This new solution has been developped to capture &nbsp;an increased number of genes for great discovery efficiency.</span></span></p>
<p><span><span>The D-Plex mRNA-seq Kit includes all buffers and enzymes necessary for mRNA capture and library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
</ul>
<div class="extra-spaced" align="center"></div>
<div class="row">
<div class="carrousel" style="background-position: center;">
<div class="slick">
<div>
<h3>Greater&nbsp;gene discovery efficiency at low inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/UHR_mRNA.png" alt="total RNA kit" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq kit enables the detection of higher numbers of genes even for low RNA inputs. Increased number of genes detected at 10x coverage is an indicator of greater sensitivity.</p>
</div>
</div>
<div>
<h3>High mappability to the coding transcriptome</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_mRNA_biotypes.png" alt="total RNA diversity" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq libraries capture efficiently coding exons and introns present in the sample of interest.</p>
</div>
</div>
<div>
<h3>Consistent, sensitive gene expression profiling</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_mRNA_violin_tpm_10.png" alt="total RNA" height="200px" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>Gene expression profiling obtained with D-Plex mRNA-seq kit is conserved across a broad dynamic range of inputs, especially for the lowest RNA inputs.</p>
</div>
</div>
<div>
<h3>Exceptional data concordance at low inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_mrna.png" alt="total RNA" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq kit produces quality data with high data concordance between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for precious samples with limited starting material.</p>
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<h2>Product Features</h2>
<ul style="list-style-type: disc;">
<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
<li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li>
<li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li>
<li><span>Get high sensitivity data even from difficult samples, such as degraded, FFPE samples</span></li>
<li><span style="font-weight: 400;">Enjoy a fast, easy, single tube protocol</span></li>
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<p></p>
<p></p>
<h4></h4>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Total RNA-seq Library Preparation Kit is a tool&nbsp;designed for the study of the whole coding and non-coding transcriptome.&nbsp;<span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span>&nbsp;</span>D-Plex technology</a><span><span>&nbsp;</span>to generate&nbsp;directional&nbsp;libraries for Illumina sequencing directly from total RNAs, mRNAs&nbsp;that has already been enriched by poly(A) selection, or RNAs&nbsp;that has already been depleted of rRNAs.</span></p>
<p><span>The D-Plex technology utilizes&nbsp;two innovative&nbsp;ligation-free mechanisms - poly(A) tailing and template switching&nbsp;- to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs.&nbsp;Combined with unique molecular identifiers (UMI), this complete solution&nbsp;delivers&nbsp;a high-sensitivity detection&nbsp;method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p>
<p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time.&nbsp;<span>The library preparation takes place in a&nbsp;</span><span>single tube</span><span>, increasing the efficiency tremendously.&nbsp;This new solution has been extensively validated for&nbsp;both intact and highly degraded RNA&nbsp;samples, including that derived from FFPE preparations.</span></span></p>
<p><span><span>D-Plex&nbsp;Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C"><span>C05030023</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set C&nbsp;&nbsp;</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set D&nbsp;</a></li>
</ul>
<div class="extra-spaced" align="center"></div>
<div class="row">
<div class="carrousel" style="background-position: center;">
<div class="slick">
<div>
<h3>Greater sensitivity to detect novel transcripts</h3>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p>
</div>
</div>
<div>
<h3>Highest diversity to facilitate biomarker discovery</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p>
</div>
</div>
<div>
<h3>Consistent expression profiling across a wide range of RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p>
</div>
</div>
<div>
<h3>Superior library complexity at low RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p>
</div>
</div>
</div>
</div>
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<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C" title="D-Plex UDI Module - Set C" target="_blank"><span>C05030023</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set C</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set D&nbsp;</a></li>
</ul>
<p></p>
<p>The use of UDI is highly recommended&nbsp;to mitigate errors introduced by read misassignment, including index&nbsp;hopping frequently observed with patterned flow cells such as Illumina&rsquo;s&nbsp;NovaSeq system.</p>',
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<p><img src="https://www.diagenode.com/img/product/kits/dplex/bioinfoPipe.png" alt="Small RNA seq Bioinformatics pipeline" width="925" height="196" /></p>',
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			'meta_description' => 'RNA-seq library preparation for Illumina sequencing - Unique D-Plex technology - Optimized for ultra-low input (50 pg total RNA) - UMI reduce PCR biases - UDI detect index hopping - Compatibility with FFPE samples - User-friendly and fast protocol',
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			'name' => 'D-Plex mRNA-seq Kit for Illumina',
			'description' => '<div class="small-12 medium-12 large-12 columns" style="border: 3px solid #B02736; padding: 10px; margin: 10px;">
<h2>Product Features</h2>
<ul style="list-style-type: disc;">
<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
<li><span>Ultra-sensitive methodology with low input capability down to 10 ng</span></li>
<li><span>High efficiency for greater gene detection and biomarker identification</span></li>
<li><span>Consistent gene expression profiling, optimal for gene signature definition</span></li>
<li><span>Precise measurement of strand orientation for anti-sense transcription detection</span></li>
</ul>
</div>
<h4></h4>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-mRNA-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>The D-Plex mRNA-seq Library Preparation Kit is a tool&nbsp;designed for gene expression analysis.&nbsp;<span>The kit utilizes the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span>&nbsp;</span>D-Plex technology</a><span><span>&nbsp;</span>to generate&nbsp;directional&nbsp;libraries for Illumina sequencing directly from total RNAs.</span></p>
<p><span>The D-Plex technology utilizes&nbsp;two innovative&nbsp;ligation-free mechanisms - poly(A) tailing and template switching&nbsp;- to produce&nbsp;sequencing libraries from low input samples, down to 10 ng of total RNAs.&nbsp;Combined with unique molecular identifiers (UMI), this complete solution&nbsp;delivers&nbsp;a high-sensitivity detection&nbsp;method for gene expression analysis and discovery applications in the coding transcriptome.</span></p>
<p><span></span><span>The D-Plex mRNA-seq Kit offers a time-saving protocol that can be completed within 6 hours and requires minimal hands-on time.&nbsp;<span>The library preparation takes place in a&nbsp;</span><span>single tube</span><span>, increasing the efficiency tremendously.&nbsp;This new solution has been developped to capture &nbsp;an increased number of genes for great discovery efficiency.</span></span></p>
<p><span><span>The D-Plex mRNA-seq Kit includes all buffers and enzymes necessary for mRNA capture and library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
</ul>
<div class="extra-spaced" align="center"></div>
<div class="row">
<div class="carrousel" style="background-position: center;">
<div class="slick">
<div>
<h3>Greater&nbsp;gene discovery efficiency at low inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/UHR_mRNA.png" alt="total RNA kit" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq kit enables the detection of higher numbers of genes even for low RNA inputs. Increased number of genes detected at 10x coverage is an indicator of greater sensitivity.</p>
</div>
</div>
<div>
<h3>High mappability to the coding transcriptome</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_mRNA_biotypes.png" alt="total RNA diversity" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq libraries capture efficiently coding exons and introns present in the sample of interest.</p>
</div>
</div>
<div>
<h3>Consistent, sensitive gene expression profiling</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_mRNA_violin_tpm_10.png" alt="total RNA" height="200px" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>Gene expression profiling obtained with D-Plex mRNA-seq kit is conserved across a broad dynamic range of inputs, especially for the lowest RNA inputs.</p>
</div>
</div>
<div>
<h3>Exceptional data concordance at low inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_mrna.png" alt="total RNA" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq kit produces quality data with high data concordance between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for precious samples with limited starting material.</p>
</div>
</div>
</div>
</div>
</div>
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<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => 'Indexes',
			'info2' => '<p><span>Specific D-Plex indexes&nbsp;</span><span>were designed and validated to fit the D-Plex technology for Illumina sequencing&nbsp;and&nbsp;</span><span>are not included in the kit. They can be bought separately according to your needs. Please choose&nbsp;the format that suits you best among the compatible references to:</span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
</ul>
<p><span></span></p>
<p><span>The use of UDI is highly recommended&nbsp;to mitigate errors introduced by read misassignment, including index&nbsp;hopping frequently observed with patterned flow cells such as Illumina&rsquo;s&nbsp;NovaSeq system.</span></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
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<p><img src="https://www.diagenode.com/img/product/kits/dplex/bioinfoPipe.png" alt="small RNA sequencing bioinformatics pipeline" caption="false" width="925" height="196" /></p>
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			'modified' => '2021-06-18 07:27:36',
			'created' => '2021-04-13 14:20:32',
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	'Application' => array(
		(int) 0 => array(
			'id' => '46',
			'position' => '0',
			'parent_id' => null,
			'name' => 'RNA-seq',
			'description' => '',
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		(int) 1 => array(
			'id' => '3',
			'position' => '10',
			'parent_id' => null,
			'name' => '次世代シーケンシング',
			'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h2 style="font-size: 22px;">DNA断片化、ライブラリー調製、自動化：NGSのワンストップショップ</h2>
<table class="small-12 medium-12 large-12 columns">
<tbody>
<tr>
<th class="small-12 medium-12 large-12 columns">
<h4>1. 断片化装置を選択してください：150 bp〜75 kbの範囲でDNAを断片化します。</h4>
</th>
</tr>
<tr style="background-color: #ffffff;">
<td class="small-12 medium-12 large-12 columns"></td>
</tr>
<tr>
<td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-pico-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/bioruptor_pico.jpg" /></a></td>
<td class="small-4 medium-4 large-4 columns"><a href="../p/megaruptor2-1-unit"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010001_megaruptor2.jpg" /></a></td>
<td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-one-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-profil.png" /></a></td>
</tr>
<tr>
<td class="small-4 medium-4 large-4 columns">5&mu;lまで断片化：150 bp〜2 kb<br />NGS DNAライブラリー調製およびFFPE核酸抽出に最適で、</td>
<td class="small-4 medium-4 large-4 columns">2 kb〜75 kbの範囲をできます。<br />メイトペアライブラリー調製および長いフラグメントDNAシーケンシングに最適で、この軽量デスクトップデバイスで</td>
<td class="small-4 medium-4 large-4 columns">20または50&mu;lの断片化が可能です。</td>
</tr>
</tbody>
</table>
<table class="small-12 medium-12 large-12 columns">
<tbody>
<tr>
<th class="small-8 medium-8 large-8 columns">
<h4>2. 最適化されたライブラリー調整キットを選択してください。</h4>
</th>
<th class="small-4 medium-4 large-4 columns">
<h4>3. ライブラリー前処理自動化を選択して、比類のないデータ再現性を実感</h4>
</th>
</tr>
<tr style="background-color: #ffffff;">
<td class="small-12 medium-12 large-12 columns"></td>
</tr>
<tr>
<td class="small-4 medium-4 large-4 columns"><a href="../p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><img src="https://www.diagenode.com/img/product/kits/microPlex_library_preparation.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td>
<td class="small-4 medium-4 large-4 columns"><a href="../p/ideal-library-preparation-kit-x24-incl-index-primer-set-1-24-rxns"><img src="https://www.diagenode.com/img/product/kits/box_kit.jpg" style="display: block; margin-left: auto; margin-right: auto;" height="173" width="250" /></a></td>
<td class="small-4 medium-4 large-4 columns"><a href="../p/sx-8g-ip-star-compact-automated-system-1-unit"><img src="https://www.diagenode.com/img/product/automation/B03000002%20_ipstar_compact.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td>
</tr>
<tr>
<td class="small-4 medium-4 large-4 columns" style="text-align: center;">50pgの低入力：MicroPlex Library Preparation Kit</td>
<td class="small-4 medium-4 large-4 columns" style="text-align: center;">5ng以上：iDeal&nbsp;Library Preparation Kit</td>
<td class="small-4 medium-4 large-4 columns" style="text-align: center;">Achieve great NGS data easily</td>
</tr>
</tbody>
</table>
</div>
</div>
<blockquote>
<div class="row">
<div class="small-12 medium-12 large-12 columns"><span class="label" style="margin-bottom: 16px; margin-left: -22px; font-size: 15px;">DiagenodeがNGS研究にぴったりなプロバイダーである理由</span>
<p>Diagenodeは15年以上もエピジェネティクス研究に専念、専門としています。 ChIP研究クロマチン用のユニークな断片化システムの開発から始まり、 専門知識を活かし、5&mu;lのせん断体積まで可能で、NGS DNAライブラリーの調製に最適な最先端DNA断片化装置の開発にたどり着きました。 我々は以来、ChIP-seq、Methyl-seq、NGSライブラリー調製用キットを研究開発し、業界をリードする免疫沈降研究と同様に、ライブラリー調製を自動化および完結させる独自の自動化システムを開発にも成功しました。</p>
<ul>
<li>信頼されるせん断装置</li>
<li>様々なインプットからのライブラリ作成キット</li>
<li>独自の自動化デバイス</li>
</ul>
</div>
</div>
</blockquote>
<div class="row">
<div class="small-12 columns">
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#panel1a">次世代シーケンシングへの理解とその専門知識</a>
<div id="panel1a" class="content">
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>次世代シーケンシング (NGS)</strong> ）は、著しいスケールとハイスループットでシーケンシングを行い、1日に数十億もの塩基生成を可能にします。 NGSのハイスループットは迅速でありながら正確で、再現性のあるデータセットを実現し、さらにシーケンシング費用を削減します。 NGSは、ゲノムシーケンシング、ゲノム再シーケンシング、デノボシーケンシング、トランスクリプトームシーケンシング、その他にDNA-タンパク質相互作用の検出やエピゲノムなどを示します。 指数関数的に増加するシーケンシングデータの需要は、計算分析の障害や解釈、データストレージなどの課題を解決します。</p>
<p>アプリケーションおよび出発物質に応じて、数百万から数十億の鋳型DNA分子を大規模に並行してシーケンシングすることが可能です。その為に、異なる化学物質を使用するいくつかの市販のNGSプラットフォームを利用することができます。 NGSプラットフォームの種類によっては、事前準備とライブラリー作成が必要です。</p>
<p>NGSにとっても、特にデータ処理と分析に関した大きな課題はあります。第3世代技術はゲノミクス研究にさらに革命を起こすであろうと大きく期待されています。</p>
</div>
</div>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<p><strong>NGS アプリケーション</strong></p>
<ul>
<li>全ゲノム配列決定</li>
<li>デノボシーケンシング</li>
<li>標的配列</li>
<li>Exomeシーケンシング</li>
<li>トランスクリプトーム配列決定</li>
<li>ゲノム配列決定</li>
<li>ミトコンドリア配列決定</li>
<li>DNA-タンパク質相互作用（ChIP-seq</li>
<li>バリアント検出</li>
<li>ゲノム仕上げ</li>
</ul>
</div>
<div class="small-6 medium-6 large-6 columns">
<p><strong>研究分野におけるNGS:</strong></p>
<ul>
<li>腫瘍学</li>
<li>リプロダクティブ・ヘルス</li>
<li>法医学ゲノミクス</li>
<li>アグリゲノミックス</li>
<li>複雑な病気</li>
<li>微生物ゲノミクス</li>
<li>食品・環境ゲノミクス</li>
<li>創薬ゲノミクス - パーソナライズド・メディカル</li>
</ul>
</div>
<div class="small-12 medium-12 large-12 columns">
<p><strong>NGSの用語</strong></p>
<dl>
<dt>リード（読み取り）</dt>
<dd>この装置から得られた連続した単一のストレッチ</dd>
<dt>断片リード</dt>
<dd>フラグメントライブラリからの読み込み。 シーケンシングプラットフォームに応じて、読み取りは通常約100〜300bp。</dd>
<dt>断片ペアエンドリード</dt>
<dd>断片ライブラリーからDNA断片の各末端2つの読み取り。</dd>
<dt>メイトペアリード</dt>
<dd>大きなDNA断片（通常は予め定義されたサイズ範囲）の各末端から2つの読み取り。</dd>
<dt>カバレッジ（例）</dt>
<dd>30&times;適用範囲とは、参照ゲノム中の各塩基対が平均30回の読み取りを示す。</dd>
</dl>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h2>NGSプラットフォーム</h2>
<h3><a href="http://www.illumina.com" target="_blank">イルミナ</a></h3>
<p>イルミナは、クローン的に増幅された鋳型DNA（クラスター）上に位置する、蛍光標識された可逆的鎖ターミネーターヌクレオチドを用いた配列別合成技術を使用。 DNAクラスターは、ガラスフローセルの表面上に固定化され、 ワークフローは、4つのヌクレオチド（それぞれ異なる蛍光色素で標識された）の組み込み、4色イメージング、色素や末端基の切断、取り込み、イメージングなどを繰り返します。フローセルは大規模な並列配列決定を受ける。 この方法により、単一蛍光標識されたヌクレオチドの制御添加によるモノヌクレオチドのエラーを回避する可能性があります。 読み取りの長さは、通常約100〜150 bpです。</p>
<h3><a href="http://www.lifetechnologies.com" target="_blank">イオン トレント</a></h3>
<p>イオントレントは、半導体技術チップを用いて、合成中にヌクレオチドを取り込む際に放出されたプロトンを検出します。 これは、イオン球粒子と呼ばれるビーズの表面にエマルションPCR（emPCR）を使用し、リンクされた特定のアダプターを用いてDNA断片を増幅します。 各ビーズは1種類のDNA断片で覆われていて、異なるDNA断片を有するビーズは次いで、チップの陽子感知ウェル内に配置されます。 チップには一度に4つのヌクレオチドのうちの1つが浸水し、このプロセスは異なるヌクレオチドで15秒ごとに繰り返されます。 配列決定の間に4つの塩基の各々が1つずつ導入されます、組み込みの場合はプロトンが放出され、電圧信号が取り込みに比例して検出されます。.</p>
<h3><a href="http://www.pacificbiosciences.com" target="_blank">パシフィック バイオサイエンス</a></h3>
<p>パシフィックバイオサイエンスでは、20kbを超える塩基対の読み取りも、単一分子リアルタイム（SMRT）シーケンシングによる構造および細胞タイプの変化を観察することができます。 このプラットフォームでは、超長鎖二本鎖DNA（dsDNA）断片が、Megaruptor（登録商標）のようなDiagenode装置を用いたランダムシアリングまたは目的の標的領域の増幅によって生成されます。 SMRTbellライブラリーは、ユニバーサルヘアピンアダプターをDNA断片の各末端に連結することによって生成します。 サイズ選択条件による洗浄ステップの後、配列決定プライマーをSMRTbellテンプレートにアニーリングし、鋳型DNAに結合したDNAポリメラーゼを含む配列決定を、蛍光標識ヌクレオチドの存在下で開始。 各塩基が取り込まれると、異なる蛍光のパルスをリアルタイムで検出します。</p>
<h3><a href="https://nanoporetech.com" target="_blank">オックスフォード ナノポア</a></h3>
<p>Oxford Nanoporeは、単一のDNA分子配列決定に基づく技術を開発します。その技術により生物学的分子、すなわちDNAが一群の電気抵抗性高分子膜として位置するナノスケールの孔（ナノ細孔）またはその近くを通過し、イオン電流が変化します。 この変化に関する情報は、例えば4つのヌクレオチド（AまたはG r CまたはT）ならびに修飾されたヌクレオチドすべてを区別することによって分子情報に訳されます。 シーケンシングミニオンデバイスのフローセルは、数百個のナノポアチャネルのセンサアレイを含みます。 DNAサンプルは、Diagenode社のMegaruptor（登録商標）を用いてランダムシアリングによって生成され得る超長鎖DNAフラグメントが必要です。</p>
<h3><a href="http://www.lifetechnologies.com/be/en/home/life-science/sequencing/next-generation-sequencing/solid-next-generation-sequencing.html" target="_blank">SOLiD</a></h3>
<p>SOLiDは、ユニークな化学作用により、何千という個々のDNA分子の同時配列決定を可能にします。 それは、アダプター対ライブラリーのフラグメントが適切で、せん断されたゲノムDNAへのアダプターのライゲーションによるライブラリー作製から始まります。 次のステップでは、エマルジョンPCR（emPCR）を実施して、ビーズの表面上の個々の鋳型DNA分子をクローン的に増幅。 emPCRでは、個々の鋳型DNAをPCR試薬と混合し、水中油型エマルジョン内の疎水性シェルで囲まれた水性液滴内のプライマーコートビーズを、配列決定のためにロードするスライドガラスの表面にランダムに付着。 この技術は、シークエンシングプライマーへのライゲーションで競合する4つの蛍光標識されたジ塩基プローブのセットを使用します。</p>
<h3><a href="http://454.com/products/technology.asp" target="_blank">454</a></h3>
<p>454は、大規模並列パイロシーケンシングを利用しています。 始めに全ゲノムDNAまたは標的遺伝子断片の300〜800bp断片のライブラリー調製します。 次に、DNAフラグメントへのアダプターの付着および単一のDNA鎖の分離。 その後アダプターに連結されたDNAフラグメントをエマルジョンベースのクローン増幅（emPCR）で処理し、DNAライブラリーフラグメントをミクロンサイズのビーズ上に配置します。 各DNA結合ビーズを光ファイバーチップ上のウェルに入れ、器具に挿入します。 4つのDNAヌクレオチドは、配列決定操作中に固定された順序で連続して加えられ、並行して配列決定されます。</p>
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<p><span style="font-weight: 400;">Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to </span><a href="../applications/dna-rna-shearing"><span style="font-weight: 400;">fragmented DNA or RNA</span></a><span style="font-weight: 400;"> prior to sequencing</span></p>
<p><span style="font-weight: 400;">After input DNA has been fragmented, it is end-repaired and blunt-ended</span><span style="font-weight: 400;">. The next step is a A-tailing in which dAMP is added to the 3&acute; end of the blunt phosphorylated DNA fragments to prevent concatemerization and to allow the ligation of adaptors with complementary dT overhangs. In addition, barcoded adapters can be incorporated to facilitate multiplexing prior to or during amplification.</span></p>
<center><img src="https://www.diagenode.com/img/categories/library-prep/flux.png" /></center>
<p><span style="font-weight: 400;">Diagenode offers a comprehensive product portfolio for library preparation:<br /></span></p>
<strong><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex&nbsp;RNA-seq Library Preparation Kits</a></strong><br />
<p><span style="font-weight: 400;">Diagenode&rsquo;s new RNA-sequencing solutions utilize the innovative c</span><span style="font-weight: 400;">apture and a</span><span style="font-weight: 400;">mplification by t</span><span style="font-weight: 400;">ailing and s</span><span style="font-weight: 400;">witching&rdquo;</span><span style="font-weight: 400;">, a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of RNA. </span><span style="font-weight: 400;"></span><a href="../categories/Library-preparation-for-RNA-seq">Learn more</a></p>
<strong><a href="../categories/library-preparation-for-ChIP-seq">ChIP-seq and DNA sequencing library preparation solutions</a></strong><br />
<p><span style="font-weight: 400;">Our kits have been optimized for DNA library preparation used for next generation sequencing for a wide range of inputs. Using a simple three-step protocols, our</span><a href="http://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><span style="font-weight: 400;"> </span></a><span style="font-weight: 400;">kits are an optimal choice for library preparation from DNA inputs down to 50 pg.&nbsp;</span><a href="../categories/library-preparation-for-ChIP-seq">Learn more</a></p>
<a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span><strong>Bioruptor Pico - short fragments</strong></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br />
<p><span style="font-weight: 400;"></span><span style="font-weight: 400;">Our well-cited Bioruptor Pico is the shearing device of choice for chromatin and DNA fragmentation. Obtain uniform and tight fragment distributions &nbsp;between 150bp -2kb. </span><a href="../p/bioruptor-pico-sonication-device">Learn more</a></p>
<strong><a href="../p/megaruptor2-1-unit"><span href="../p/bioruptor-pico-sonication-device">Megaruptor</span>&reg; - long fragments</a></strong><a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br />
<p><span style="font-weight: 400;"></span><span style="font-weight: 400;">The Megaruptor is designed to shear DNA from 3kb-75kb for long-read sequencing.&nbsp; <a href="../p/megaruptor2-1-unit">Learn more</a></span></p>
<span href="../p/bioruptor-pico-sonication-device"></span><span style="font-weight: 400;"></span></div>
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<h2>Product Features</h2>
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<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
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<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-mRNA-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
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<p>The D-Plex mRNA-seq Library Preparation Kit is a tool&nbsp;designed for gene expression analysis.&nbsp;<span>The kit utilizes the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span>&nbsp;</span>D-Plex technology</a><span><span>&nbsp;</span>to generate&nbsp;directional&nbsp;libraries for Illumina sequencing directly from total RNAs.</span></p>
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<p><span></span><span>The D-Plex mRNA-seq Kit offers a time-saving protocol that can be completed within 6 hours and requires minimal hands-on time.&nbsp;<span>The library preparation takes place in a&nbsp;</span><span>single tube</span><span>, increasing the efficiency tremendously.&nbsp;This new solution has been developped to capture &nbsp;an increased number of genes for great discovery efficiency.</span></span></p>
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<ul>
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<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
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<p>Gene expression profiling obtained with D-Plex mRNA-seq kit is conserved across a broad dynamic range of inputs, especially for the lowest RNA inputs.</p>
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<h3>Exceptional data concordance at low inputs</h3>
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<p>D-Plex mRNA-seq kit produces quality data with high data concordance between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for precious samples with limited starting material.</p>
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<li><b>Achieve highly effective purification:</b><span><span>&nbsp;</span>Generate mRNA sample that is intact and free of DNAs and other RNAs.</span></li>
</ul>
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			'description' => '<div class="small-12 medium-12 large-12 columns" style="border: 3px solid #B02736; padding: 10px; margin: 10px;">
<h2>Product Features</h2>
<ul style="list-style-type: disc;">
<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
<li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li>
<li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li>
<li><span>Get high sensitivity data even from difficult samples, such as degraded, FFPE samples</span></li>
<li><span style="font-weight: 400;">Enjoy a fast, easy, single tube protocol</span></li>
</ul>
</div>
<p></p>
<p></p>
<h4></h4>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Total RNA-seq Library Preparation Kit is a tool&nbsp;designed for the study of the whole coding and non-coding transcriptome.&nbsp;<span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span>&nbsp;</span>D-Plex technology</a><span><span>&nbsp;</span>to generate&nbsp;directional&nbsp;libraries for Illumina sequencing directly from total RNAs, mRNAs&nbsp;that has already been enriched by poly(A) selection, or RNAs&nbsp;that has already been depleted of rRNAs.</span></p>
<p><span>The D-Plex technology utilizes&nbsp;two innovative&nbsp;ligation-free mechanisms - poly(A) tailing and template switching&nbsp;- to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs.&nbsp;Combined with unique molecular identifiers (UMI), this complete solution&nbsp;delivers&nbsp;a high-sensitivity detection&nbsp;method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p>
<p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time.&nbsp;<span>The library preparation takes place in a&nbsp;</span><span>single tube</span><span>, increasing the efficiency tremendously.&nbsp;This new solution has been extensively validated for&nbsp;both intact and highly degraded RNA&nbsp;samples, including that derived from FFPE preparations.</span></span></p>
<p><span><span>D-Plex&nbsp;Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C"><span>C05030023</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set C&nbsp;&nbsp;</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set D&nbsp;</a></li>
</ul>
<div class="extra-spaced" align="center"></div>
<div class="row">
<div class="carrousel" style="background-position: center;">
<div class="slick">
<div>
<h3>Greater sensitivity to detect novel transcripts</h3>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p>
</div>
</div>
<div>
<h3>Highest diversity to facilitate biomarker discovery</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p>
</div>
</div>
<div>
<h3>Consistent expression profiling across a wide range of RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p>
</div>
</div>
<div>
<h3>Superior library complexity at low RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p>
</div>
</div>
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<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-C" title="D-Plex UDI Module - Set C" target="_blank"><span>C05030023</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set C</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-D" title="D-Plex UDI Module - Set D" target="_blank"><span>C05030024</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set D&nbsp;</a></li>
</ul>
<p></p>
<p>The use of UDI is highly recommended&nbsp;to mitigate errors introduced by read misassignment, including index&nbsp;hopping frequently observed with patterned flow cells such as Illumina&rsquo;s&nbsp;NovaSeq system.</p>',
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			'description' => '<div class="small-12 medium-12 large-12 columns" style="border: 3px solid #B02736; padding: 10px; margin: 10px;">
<h2>Product Features</h2>
<ul style="list-style-type: disc;">
<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
<li><span>Ultra-sensitive methodology with low input capability down to 10 ng</span></li>
<li><span>High efficiency for greater gene detection and biomarker identification</span></li>
<li><span>Consistent gene expression profiling, optimal for gene signature definition</span></li>
<li><span>Precise measurement of strand orientation for anti-sense transcription detection</span></li>
</ul>
</div>
<h4></h4>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-mRNA-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>The D-Plex mRNA-seq Library Preparation Kit is a tool&nbsp;designed for gene expression analysis.&nbsp;<span>The kit utilizes the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span>&nbsp;</span>D-Plex technology</a><span><span>&nbsp;</span>to generate&nbsp;directional&nbsp;libraries for Illumina sequencing directly from total RNAs.</span></p>
<p><span>The D-Plex technology utilizes&nbsp;two innovative&nbsp;ligation-free mechanisms - poly(A) tailing and template switching&nbsp;- to produce&nbsp;sequencing libraries from low input samples, down to 10 ng of total RNAs.&nbsp;Combined with unique molecular identifiers (UMI), this complete solution&nbsp;delivers&nbsp;a high-sensitivity detection&nbsp;method for gene expression analysis and discovery applications in the coding transcriptome.</span></p>
<p><span></span><span>The D-Plex mRNA-seq Kit offers a time-saving protocol that can be completed within 6 hours and requires minimal hands-on time.&nbsp;<span>The library preparation takes place in a&nbsp;</span><span>single tube</span><span>, increasing the efficiency tremendously.&nbsp;This new solution has been developped to capture &nbsp;an increased number of genes for great discovery efficiency.</span></span></p>
<p><span><span>The D-Plex mRNA-seq Kit includes all buffers and enzymes necessary for mRNA capture and library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
</ul>
<div class="extra-spaced" align="center"></div>
<div class="row">
<div class="carrousel" style="background-position: center;">
<div class="slick">
<div>
<h3>Greater&nbsp;gene discovery efficiency at low inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/UHR_mRNA.png" alt="total RNA kit" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq kit enables the detection of higher numbers of genes even for low RNA inputs. Increased number of genes detected at 10x coverage is an indicator of greater sensitivity.</p>
</div>
</div>
<div>
<h3>High mappability to the coding transcriptome</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_mRNA_biotypes.png" alt="total RNA diversity" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq libraries capture efficiently coding exons and introns present in the sample of interest.</p>
</div>
</div>
<div>
<h3>Consistent, sensitive gene expression profiling</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_mRNA_violin_tpm_10.png" alt="total RNA" height="200px" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>Gene expression profiling obtained with D-Plex mRNA-seq kit is conserved across a broad dynamic range of inputs, especially for the lowest RNA inputs.</p>
</div>
</div>
<div>
<h3>Exceptional data concordance at low inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_mrna.png" alt="total RNA" /></center></div>
<div class="large-6 small-12 medium-6 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex mRNA-seq kit produces quality data with high data concordance between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for precious samples with limited starting material.</p>
</div>
</div>
</div>
</div>
</div>
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<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => 'Indexes',
			'info2' => '<p><span>Specific D-Plex indexes&nbsp;</span><span>were designed and validated to fit the D-Plex technology for Illumina sequencing&nbsp;and&nbsp;</span><span>are not included in the kit. They can be bought separately according to your needs. Please choose&nbsp;the format that suits you best among the compatible references to:</span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
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<p><span></span></p>
<p><span>The use of UDI is highly recommended&nbsp;to mitigate errors introduced by read misassignment, including index&nbsp;hopping frequently observed with patterned flow cells such as Illumina&rsquo;s&nbsp;NovaSeq system.</span></p>
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<p><img src="https://www.diagenode.com/img/product/kits/dplex/bioinfoPipe.png" alt="small RNA sequencing bioinformatics pipeline" caption="false" width="925" height="196" /></p>
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<div class="small-12 medium-12 large-12 columns">
<h2 style="font-size: 22px;">DNA断片化、ライブラリー調製、自動化：NGSのワンストップショップ</h2>
<table class="small-12 medium-12 large-12 columns">
<tbody>
<tr>
<th class="small-12 medium-12 large-12 columns">
<h4>1. 断片化装置を選択してください：150 bp〜75 kbの範囲でDNAを断片化します。</h4>
</th>
</tr>
<tr style="background-color: #ffffff;">
<td class="small-12 medium-12 large-12 columns"></td>
</tr>
<tr>
<td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-pico-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/bioruptor_pico.jpg" /></a></td>
<td class="small-4 medium-4 large-4 columns"><a href="../p/megaruptor2-1-unit"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010001_megaruptor2.jpg" /></a></td>
<td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-one-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-profil.png" /></a></td>
</tr>
<tr>
<td class="small-4 medium-4 large-4 columns">5&mu;lまで断片化：150 bp〜2 kb<br />NGS DNAライブラリー調製およびFFPE核酸抽出に最適で、</td>
<td class="small-4 medium-4 large-4 columns">2 kb〜75 kbの範囲をできます。<br />メイトペアライブラリー調製および長いフラグメントDNAシーケンシングに最適で、この軽量デスクトップデバイスで</td>
<td class="small-4 medium-4 large-4 columns">20または50&mu;lの断片化が可能です。</td>
</tr>
</tbody>
</table>
<table class="small-12 medium-12 large-12 columns">
<tbody>
<tr>
<th class="small-8 medium-8 large-8 columns">
<h4>2. 最適化されたライブラリー調整キットを選択してください。</h4>
</th>
<th class="small-4 medium-4 large-4 columns">
<h4>3. ライブラリー前処理自動化を選択して、比類のないデータ再現性を実感</h4>
</th>
</tr>
<tr style="background-color: #ffffff;">
<td class="small-12 medium-12 large-12 columns"></td>
</tr>
<tr>
<td class="small-4 medium-4 large-4 columns"><a href="../p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><img src="https://www.diagenode.com/img/product/kits/microPlex_library_preparation.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td>
<td class="small-4 medium-4 large-4 columns"><a href="../p/ideal-library-preparation-kit-x24-incl-index-primer-set-1-24-rxns"><img src="https://www.diagenode.com/img/product/kits/box_kit.jpg" style="display: block; margin-left: auto; margin-right: auto;" height="173" width="250" /></a></td>
<td class="small-4 medium-4 large-4 columns"><a href="../p/sx-8g-ip-star-compact-automated-system-1-unit"><img src="https://www.diagenode.com/img/product/automation/B03000002%20_ipstar_compact.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td>
</tr>
<tr>
<td class="small-4 medium-4 large-4 columns" style="text-align: center;">50pgの低入力：MicroPlex Library Preparation Kit</td>
<td class="small-4 medium-4 large-4 columns" style="text-align: center;">5ng以上：iDeal&nbsp;Library Preparation Kit</td>
<td class="small-4 medium-4 large-4 columns" style="text-align: center;">Achieve great NGS data easily</td>
</tr>
</tbody>
</table>
</div>
</div>
<blockquote>
<div class="row">
<div class="small-12 medium-12 large-12 columns"><span class="label" style="margin-bottom: 16px; margin-left: -22px; font-size: 15px;">DiagenodeがNGS研究にぴったりなプロバイダーである理由</span>
<p>Diagenodeは15年以上もエピジェネティクス研究に専念、専門としています。 ChIP研究クロマチン用のユニークな断片化システムの開発から始まり、 専門知識を活かし、5&mu;lのせん断体積まで可能で、NGS DNAライブラリーの調製に最適な最先端DNA断片化装置の開発にたどり着きました。 我々は以来、ChIP-seq、Methyl-seq、NGSライブラリー調製用キットを研究開発し、業界をリードする免疫沈降研究と同様に、ライブラリー調製を自動化および完結させる独自の自動化システムを開発にも成功しました。</p>
<ul>
<li>信頼されるせん断装置</li>
<li>様々なインプットからのライブラリ作成キット</li>
<li>独自の自動化デバイス</li>
</ul>
</div>
</div>
</blockquote>
<div class="row">
<div class="small-12 columns">
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#panel1a">次世代シーケンシングへの理解とその専門知識</a>
<div id="panel1a" class="content">
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>次世代シーケンシング (NGS)</strong> ）は、著しいスケールとハイスループットでシーケンシングを行い、1日に数十億もの塩基生成を可能にします。 NGSのハイスループットは迅速でありながら正確で、再現性のあるデータセットを実現し、さらにシーケンシング費用を削減します。 NGSは、ゲノムシーケンシング、ゲノム再シーケンシング、デノボシーケンシング、トランスクリプトームシーケンシング、その他にDNA-タンパク質相互作用の検出やエピゲノムなどを示します。 指数関数的に増加するシーケンシングデータの需要は、計算分析の障害や解釈、データストレージなどの課題を解決します。</p>
<p>アプリケーションおよび出発物質に応じて、数百万から数十億の鋳型DNA分子を大規模に並行してシーケンシングすることが可能です。その為に、異なる化学物質を使用するいくつかの市販のNGSプラットフォームを利用することができます。 NGSプラットフォームの種類によっては、事前準備とライブラリー作成が必要です。</p>
<p>NGSにとっても、特にデータ処理と分析に関した大きな課題はあります。第3世代技術はゲノミクス研究にさらに革命を起こすであろうと大きく期待されています。</p>
</div>
</div>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<p><strong>NGS アプリケーション</strong></p>
<ul>
<li>全ゲノム配列決定</li>
<li>デノボシーケンシング</li>
<li>標的配列</li>
<li>Exomeシーケンシング</li>
<li>トランスクリプトーム配列決定</li>
<li>ゲノム配列決定</li>
<li>ミトコンドリア配列決定</li>
<li>DNA-タンパク質相互作用（ChIP-seq</li>
<li>バリアント検出</li>
<li>ゲノム仕上げ</li>
</ul>
</div>
<div class="small-6 medium-6 large-6 columns">
<p><strong>研究分野におけるNGS:</strong></p>
<ul>
<li>腫瘍学</li>
<li>リプロダクティブ・ヘルス</li>
<li>法医学ゲノミクス</li>
<li>アグリゲノミックス</li>
<li>複雑な病気</li>
<li>微生物ゲノミクス</li>
<li>食品・環境ゲノミクス</li>
<li>創薬ゲノミクス - パーソナライズド・メディカル</li>
</ul>
</div>
<div class="small-12 medium-12 large-12 columns">
<p><strong>NGSの用語</strong></p>
<dl>
<dt>リード（読み取り）</dt>
<dd>この装置から得られた連続した単一のストレッチ</dd>
<dt>断片リード</dt>
<dd>フラグメントライブラリからの読み込み。 シーケンシングプラットフォームに応じて、読み取りは通常約100〜300bp。</dd>
<dt>断片ペアエンドリード</dt>
<dd>断片ライブラリーからDNA断片の各末端2つの読み取り。</dd>
<dt>メイトペアリード</dt>
<dd>大きなDNA断片（通常は予め定義されたサイズ範囲）の各末端から2つの読み取り。</dd>
<dt>カバレッジ（例）</dt>
<dd>30&times;適用範囲とは、参照ゲノム中の各塩基対が平均30回の読み取りを示す。</dd>
</dl>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h2>NGSプラットフォーム</h2>
<h3><a href="http://www.illumina.com" target="_blank">イルミナ</a></h3>
<p>イルミナは、クローン的に増幅された鋳型DNA（クラスター）上に位置する、蛍光標識された可逆的鎖ターミネーターヌクレオチドを用いた配列別合成技術を使用。 DNAクラスターは、ガラスフローセルの表面上に固定化され、 ワークフローは、4つのヌクレオチド（それぞれ異なる蛍光色素で標識された）の組み込み、4色イメージング、色素や末端基の切断、取り込み、イメージングなどを繰り返します。フローセルは大規模な並列配列決定を受ける。 この方法により、単一蛍光標識されたヌクレオチドの制御添加によるモノヌクレオチドのエラーを回避する可能性があります。 読み取りの長さは、通常約100〜150 bpです。</p>
<h3><a href="http://www.lifetechnologies.com" target="_blank">イオン トレント</a></h3>
<p>イオントレントは、半導体技術チップを用いて、合成中にヌクレオチドを取り込む際に放出されたプロトンを検出します。 これは、イオン球粒子と呼ばれるビーズの表面にエマルションPCR（emPCR）を使用し、リンクされた特定のアダプターを用いてDNA断片を増幅します。 各ビーズは1種類のDNA断片で覆われていて、異なるDNA断片を有するビーズは次いで、チップの陽子感知ウェル内に配置されます。 チップには一度に4つのヌクレオチドのうちの1つが浸水し、このプロセスは異なるヌクレオチドで15秒ごとに繰り返されます。 配列決定の間に4つの塩基の各々が1つずつ導入されます、組み込みの場合はプロトンが放出され、電圧信号が取り込みに比例して検出されます。.</p>
<h3><a href="http://www.pacificbiosciences.com" target="_blank">パシフィック バイオサイエンス</a></h3>
<p>パシフィックバイオサイエンスでは、20kbを超える塩基対の読み取りも、単一分子リアルタイム（SMRT）シーケンシングによる構造および細胞タイプの変化を観察することができます。 このプラットフォームでは、超長鎖二本鎖DNA（dsDNA）断片が、Megaruptor（登録商標）のようなDiagenode装置を用いたランダムシアリングまたは目的の標的領域の増幅によって生成されます。 SMRTbellライブラリーは、ユニバーサルヘアピンアダプターをDNA断片の各末端に連結することによって生成します。 サイズ選択条件による洗浄ステップの後、配列決定プライマーをSMRTbellテンプレートにアニーリングし、鋳型DNAに結合したDNAポリメラーゼを含む配列決定を、蛍光標識ヌクレオチドの存在下で開始。 各塩基が取り込まれると、異なる蛍光のパルスをリアルタイムで検出します。</p>
<h3><a href="https://nanoporetech.com" target="_blank">オックスフォード ナノポア</a></h3>
<p>Oxford Nanoporeは、単一のDNA分子配列決定に基づく技術を開発します。その技術により生物学的分子、すなわちDNAが一群の電気抵抗性高分子膜として位置するナノスケールの孔（ナノ細孔）またはその近くを通過し、イオン電流が変化します。 この変化に関する情報は、例えば4つのヌクレオチド（AまたはG r CまたはT）ならびに修飾されたヌクレオチドすべてを区別することによって分子情報に訳されます。 シーケンシングミニオンデバイスのフローセルは、数百個のナノポアチャネルのセンサアレイを含みます。 DNAサンプルは、Diagenode社のMegaruptor（登録商標）を用いてランダムシアリングによって生成され得る超長鎖DNAフラグメントが必要です。</p>
<h3><a href="http://www.lifetechnologies.com/be/en/home/life-science/sequencing/next-generation-sequencing/solid-next-generation-sequencing.html" target="_blank">SOLiD</a></h3>
<p>SOLiDは、ユニークな化学作用により、何千という個々のDNA分子の同時配列決定を可能にします。 それは、アダプター対ライブラリーのフラグメントが適切で、せん断されたゲノムDNAへのアダプターのライゲーションによるライブラリー作製から始まります。 次のステップでは、エマルジョンPCR（emPCR）を実施して、ビーズの表面上の個々の鋳型DNA分子をクローン的に増幅。 emPCRでは、個々の鋳型DNAをPCR試薬と混合し、水中油型エマルジョン内の疎水性シェルで囲まれた水性液滴内のプライマーコートビーズを、配列決定のためにロードするスライドガラスの表面にランダムに付着。 この技術は、シークエンシングプライマーへのライゲーションで競合する4つの蛍光標識されたジ塩基プローブのセットを使用します。</p>
<h3><a href="http://454.com/products/technology.asp" target="_blank">454</a></h3>
<p>454は、大規模並列パイロシーケンシングを利用しています。 始めに全ゲノムDNAまたは標的遺伝子断片の300〜800bp断片のライブラリー調製します。 次に、DNAフラグメントへのアダプターの付着および単一のDNA鎖の分離。 その後アダプターに連結されたDNAフラグメントをエマルジョンベースのクローン増幅（emPCR）で処理し、DNAライブラリーフラグメントをミクロンサイズのビーズ上に配置します。 各DNA結合ビーズを光ファイバーチップ上のウェルに入れ、器具に挿入します。 4つのDNAヌクレオチドは、配列決定操作中に固定された順序で連続して加えられ、並行して配列決定されます。</p>
</div>
</div>
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<p><span style="font-weight: 400;">Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to </span><a href="../applications/dna-rna-shearing"><span style="font-weight: 400;">fragmented DNA or RNA</span></a><span style="font-weight: 400;"> prior to sequencing</span></p>
<p><span style="font-weight: 400;">After input DNA has been fragmented, it is end-repaired and blunt-ended</span><span style="font-weight: 400;">. The next step is a A-tailing in which dAMP is added to the 3&acute; end of the blunt phosphorylated DNA fragments to prevent concatemerization and to allow the ligation of adaptors with complementary dT overhangs. In addition, barcoded adapters can be incorporated to facilitate multiplexing prior to or during amplification.</span></p>
<center><img src="https://www.diagenode.com/img/categories/library-prep/flux.png" /></center>
<p><span style="font-weight: 400;">Diagenode offers a comprehensive product portfolio for library preparation:<br /></span></p>
<strong><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex&nbsp;RNA-seq Library Preparation Kits</a></strong><br />
<p><span style="font-weight: 400;">Diagenode&rsquo;s new RNA-sequencing solutions utilize the innovative c</span><span style="font-weight: 400;">apture and a</span><span style="font-weight: 400;">mplification by t</span><span style="font-weight: 400;">ailing and s</span><span style="font-weight: 400;">witching&rdquo;</span><span style="font-weight: 400;">, a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of RNA. </span><span style="font-weight: 400;"></span><a href="../categories/Library-preparation-for-RNA-seq">Learn more</a></p>
<strong><a href="../categories/library-preparation-for-ChIP-seq">ChIP-seq and DNA sequencing library preparation solutions</a></strong><br />
<p><span style="font-weight: 400;">Our kits have been optimized for DNA library preparation used for next generation sequencing for a wide range of inputs. Using a simple three-step protocols, our</span><a href="http://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><span style="font-weight: 400;"> </span></a><span style="font-weight: 400;">kits are an optimal choice for library preparation from DNA inputs down to 50 pg.&nbsp;</span><a href="../categories/library-preparation-for-ChIP-seq">Learn more</a></p>
<a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span><strong>Bioruptor Pico - short fragments</strong></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br />
<p><span style="font-weight: 400;"></span><span style="font-weight: 400;">Our well-cited Bioruptor Pico is the shearing device of choice for chromatin and DNA fragmentation. Obtain uniform and tight fragment distributions &nbsp;between 150bp -2kb. </span><a href="../p/bioruptor-pico-sonication-device">Learn more</a></p>
<strong><a href="../p/megaruptor2-1-unit"><span href="../p/bioruptor-pico-sonication-device">Megaruptor</span>&reg; - long fragments</a></strong><a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br />
<p><span style="font-weight: 400;"></span><span style="font-weight: 400;">The Megaruptor is designed to shear DNA from 3kb-75kb for long-read sequencing.&nbsp; <a href="../p/megaruptor2-1-unit">Learn more</a></span></p>
<span href="../p/bioruptor-pico-sonication-device"></span><span style="font-weight: 400;"></span></div>
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<h2>Product Features</h2>
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<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
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<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-mRNA-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
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<p>The D-Plex mRNA-seq Library Preparation Kit is a tool&nbsp;designed for gene expression analysis.&nbsp;<span>The kit utilizes the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span>&nbsp;</span>D-Plex technology</a><span><span>&nbsp;</span>to generate&nbsp;directional&nbsp;libraries for Illumina sequencing directly from total RNAs.</span></p>
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<p><span></span><span>The D-Plex mRNA-seq Kit offers a time-saving protocol that can be completed within 6 hours and requires minimal hands-on time.&nbsp;<span>The library preparation takes place in a&nbsp;</span><span>single tube</span><span>, increasing the efficiency tremendously.&nbsp;This new solution has been developped to capture &nbsp;an increased number of genes for great discovery efficiency.</span></span></p>
<p><span><span>The D-Plex mRNA-seq Kit includes all buffers and enzymes necessary for mRNA capture and library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
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<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span>&nbsp;- D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
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<p>Gene expression profiling obtained with D-Plex mRNA-seq kit is conserved across a broad dynamic range of inputs, especially for the lowest RNA inputs.</p>
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<h3>Exceptional data concordance at low inputs</h3>
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<p>D-Plex mRNA-seq kit produces quality data with high data concordance between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for precious samples with limited starting material.</p>
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