| ELISA Enzyme-linked immunosorbent assay. Read more |
| RIP RIP Read more |
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Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.
| Lot | 001 |
|---|---|
| Concentration | 0.7 µg/µl |
| Species reactivity | Human, other (wide range): positive. |
| Type | Monoclonal |
| Purity | Protein G purified monoclonal antibody |
| Host | Mouse |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% sodium azide |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| RIP* | 2 µg per IP | Fig 1 |
| ELISA | 1:1,000 | Fig 2 |
*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.
Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C
RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C
To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
| ac4C antibody DATASHEET | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: ac4C Antibody (sample size) (Diagenode Cat# C15200252-10 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 14:22:41', 'created' => '2021-12-23 14:19:14', 'select_label' => '648 - ac4C monoclonal antibody (001 - 0.7 µg/µl - Human, other (wide range): positive. - Protein G purified monoclonal antibody - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/en/p/ac4c-monoclonal-antibody' ) $language = 'en' $meta_keywords = '' $meta_description = '' $meta_title = '' $product = array( 'Product' => array( 'id' => '3209', 'antibody_id' => '648', 'name' => 'ac4C Antibody (sample size)', 'description' => '<p><span>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig1-rip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig2-elisa.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="row"> <div class="small-6 columns"> <p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.</p> </div> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200252-10', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000582', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody-10ul', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:27:10', 'created' => '2022-01-05 10:27:10', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '648', 'name' => 'ac4C monoclonal antibody', 'description' => 'N4-acetylcytidine (ac4C) is the only acetylation that has been described in eukaryotic RNA. 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RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig2-elisa.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="row"> <div class="small-6 columns"> <p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. 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These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'rna-modifications-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody', 'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies', 'meta_title' => 'RNA modifications antibodies | Diagenode.com', 'modified' => '2020-09-01 11:43:28', 'created' => '2016-02-15 10:58:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? 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RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig2-elisa.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="row"> <div class="small-6 columns"> <p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.</p> </div> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200252', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:22:01', 'created' => '2021-12-23 14:21:52' ) ) $pro = array( 'id' => '3209', 'antibody_id' => '648', 'name' => 'ac4C Antibody (sample size)', 'description' => '<p><span>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200252-10', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000582', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody-10ul', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:27:10', 'created' => '2022-01-05 10:27:10', 'ProductsGroup' => array( 'id' => '401', 'product_id' => '3209', 'group_id' => '351' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '3208', 'antibody_id' => '648', 'name' => 'ac4C Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig1-rip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig2-elisa.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="row"> <div class="small-6 columns"> <p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.</p> </div> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200252', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:22:01', 'created' => '2021-12-23 14:21:52' ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'ProductsApplication' => array( 'id' => '5498', 'product_id' => '3209', 'application_id' => '36' ) ) $slugs = array( (int) 0 => 'rip' ) $applications = array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'locale' => 'eng' ) $description = '<p>RIP</p>' $name = 'RIP' $document = array( 'id' => '1147', 'name' => 'ac4C antibody', 'description' => '', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_ac4C_C15200252.pdf', 'slug' => 'datasheet-ac4c-c15200252', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 14:43:31', 'created' => '2021-12-23 14:43:14', 'ProductsDocument' => array( 'id' => '3180', 'product_id' => '3209', 'document_id' => '1147' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => '', 'product' => array( 'Product' => array( 'id' => '3209', 'antibody_id' => '648', 'name' => 'ac4C Antibody (sample size)', 'description' => '<p><span>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200252-10', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000582', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody-10ul', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:27:10', 'created' => '2022-01-05 10:27:10', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '648', 'name' => 'ac4C monoclonal antibody', 'description' => 'N4-acetylcytidine (ac4C) is the only acetylation that has been described in eukaryotic RNA. It’s a highly conserved RNA modification which is present in mRNA, tRNA and rRNA. ac4C has been shown to have important regulatory functions and is involved in the regulation of mRNA stability and in RNA processing. Moreover, ac4C promotes translation efficiency. ', 'clonality' => '', 'isotype' => ' IgG1', 'lot' => '001', 'concentration' => '0.7 µg/µl', 'reactivity' => 'Human, other (wide range): positive.', 'type' => 'Monoclonal', 'purity' => 'Protein G purified monoclonal antibody', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP*</td> <td>2 µg per IP</td> <td>Fig 1</td> </tr> <tr> <td>ELISA</td> <td>1:1,000</td> <td>Fig 2</td> </tr> </tbody> </table> <p><small><sup>*</sup>Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% sodium azide', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 14:22:41', 'created' => '2021-12-23 14:19:14', 'select_label' => '648 - ac4C monoclonal antibody (001 - 0.7 µg/µl - Human, other (wide range): positive. - Protein G purified monoclonal antibody - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/en/p/ac4c-monoclonal-antibody' ) $language = 'en' $meta_keywords = '' $meta_description = '' $meta_title = '' $product = array( 'Product' => array( 'id' => '3209', 'antibody_id' => '648', 'name' => 'ac4C Antibody (sample size)', 'description' => '<p><span>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig1-rip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig2-elisa.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="row"> <div class="small-6 columns"> <p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.</p> </div> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200252-10', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000582', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody-10ul', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:27:10', 'created' => '2022-01-05 10:27:10', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '648', 'name' => 'ac4C monoclonal antibody', 'description' => 'N4-acetylcytidine (ac4C) is the only acetylation that has been described in eukaryotic RNA. It’s a highly conserved RNA modification which is present in mRNA, tRNA and rRNA. ac4C has been shown to have important regulatory functions and is involved in the regulation of mRNA stability and in RNA processing. Moreover, ac4C promotes translation efficiency. ', 'clonality' => '', 'isotype' => ' IgG1', 'lot' => '001', 'concentration' => '0.7 µg/µl', 'reactivity' => 'Human, other (wide range): positive.', 'type' => 'Monoclonal', 'purity' => 'Protein G purified monoclonal antibody', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP*</td> <td>2 µg per IP</td> <td>Fig 1</td> </tr> <tr> <td>ELISA</td> <td>1:1,000</td> <td>Fig 2</td> </tr> </tbody> </table> <p><small><sup>*</sup>Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% sodium azide', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 14:22:41', 'created' => '2021-12-23 14:19:14', 'select_label' => '648 - ac4C monoclonal antibody (001 - 0.7 µg/µl - Human, other (wide range): positive. - Protein G purified monoclonal antibody - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '351', 'name' => 'C15200252', 'product_id' => '3208', 'modified' => '2022-01-05 10:27:41', 'created' => '2022-01-05 10:27:41' ), 'Master' => array( 'id' => '3208', 'antibody_id' => '648', 'name' => 'ac4C Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig1-rip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig2-elisa.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="row"> <div class="small-6 columns"> <p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.</p> </div> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200252', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:22:01', 'created' => '2021-12-23 14:21:52' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '109', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA modifications', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'rna-modifications-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody', 'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies', 'meta_title' => 'RNA modifications antibodies | Diagenode.com', 'modified' => '2020-09-01 11:43:28', 'created' => '2016-02-15 10:58:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. 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All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '1147', 'name' => 'ac4C antibody', 'description' => '', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_ac4C_C15200252.pdf', 'slug' => 'datasheet-ac4c-c15200252', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 14:43:31', 'created' => '2021-12-23 14:43:14', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/ac4c-monoclonal-antibody' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '3208', 'antibody_id' => '648', 'name' => 'ac4C Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig1-rip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig2-elisa.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="row"> <div class="small-6 columns"> <p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.</p> </div> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200252', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:22:01', 'created' => '2021-12-23 14:21:52' ) ) $pro = array( 'id' => '3209', 'antibody_id' => '648', 'name' => 'ac4C Antibody (sample size)', 'description' => '<p><span>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200252-10', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000582', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody-10ul', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:27:10', 'created' => '2022-01-05 10:27:10', 'ProductsGroup' => array( 'id' => '401', 'product_id' => '3209', 'group_id' => '351' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '3208', 'antibody_id' => '648', 'name' => 'ac4C Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig1-rip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig2-elisa.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="row"> <div class="small-6 columns"> <p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.</p> </div> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200252', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:22:01', 'created' => '2021-12-23 14:21:52' ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'ProductsApplication' => array( 'id' => '5498', 'product_id' => '3209', 'application_id' => '36' ) ) $slugs = array( (int) 0 => 'rip' ) $applications = array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'locale' => 'eng' ) $description = '<p>RIP</p>' $name = 'RIP' $document = array( 'id' => '1147', 'name' => 'ac4C antibody', 'description' => '', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_ac4C_C15200252.pdf', 'slug' => 'datasheet-ac4c-c15200252', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 14:43:31', 'created' => '2021-12-23 14:43:14', 'ProductsDocument' => array( 'id' => '3180', 'product_id' => '3209', 'document_id' => '1147' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => '', 'product' => array( 'Product' => array( 'id' => '3209', 'antibody_id' => '648', 'name' => 'ac4C Antibody (sample size)', 'description' => '<p><span>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200252-10', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000582', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody-10ul', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:27:10', 'created' => '2022-01-05 10:27:10', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '648', 'name' => 'ac4C monoclonal antibody', 'description' => 'N4-acetylcytidine (ac4C) is the only acetylation that has been described in eukaryotic RNA. It’s a highly conserved RNA modification which is present in mRNA, tRNA and rRNA. ac4C has been shown to have important regulatory functions and is involved in the regulation of mRNA stability and in RNA processing. Moreover, ac4C promotes translation efficiency. ', 'clonality' => '', 'isotype' => ' IgG1', 'lot' => '001', 'concentration' => '0.7 µg/µl', 'reactivity' => 'Human, other (wide range): positive.', 'type' => 'Monoclonal', 'purity' => 'Protein G purified monoclonal antibody', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP*</td> <td>2 µg per IP</td> <td>Fig 1</td> </tr> <tr> <td>ELISA</td> <td>1:1,000</td> <td>Fig 2</td> </tr> </tbody> </table> <p><small><sup>*</sup>Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% sodium azide', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 14:22:41', 'created' => '2021-12-23 14:19:14', 'select_label' => '648 - ac4C monoclonal antibody (001 - 0.7 µg/µl - Human, other (wide range): positive. - Protein G purified monoclonal antibody - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/en/p/ac4c-monoclonal-antibody' ) $language = 'en' $meta_keywords = '' $meta_description = '' $meta_title = '' $product = array( 'Product' => array( 'id' => '3209', 'antibody_id' => '648', 'name' => 'ac4C Antibody (sample size)', 'description' => '<p><span>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig1-rip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig2-elisa.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="row"> <div class="small-6 columns"> <p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.</p> </div> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200252-10', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000582', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody-10ul', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:27:10', 'created' => '2022-01-05 10:27:10', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '648', 'name' => 'ac4C monoclonal antibody', 'description' => 'N4-acetylcytidine (ac4C) is the only acetylation that has been described in eukaryotic RNA. 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We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% sodium azide', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 14:22:41', 'created' => '2021-12-23 14:19:14', 'select_label' => '648 - ac4C monoclonal antibody (001 - 0.7 µg/µl - Human, other (wide range): positive. - Protein G purified monoclonal antibody - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '351', 'name' => 'C15200252', 'product_id' => '3208', 'modified' => '2022-01-05 10:27:41', 'created' => '2022-01-05 10:27:41' ), 'Master' => array( 'id' => '3208', 'antibody_id' => '648', 'name' => 'ac4C Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig1-rip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig2-elisa.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="row"> <div class="small-6 columns"> <p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.</p> </div> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200252', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:22:01', 'created' => '2021-12-23 14:21:52' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '109', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA modifications', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'rna-modifications-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody', 'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies', 'meta_title' => 'RNA modifications antibodies | Diagenode.com', 'modified' => '2020-09-01 11:43:28', 'created' => '2016-02-15 10:58:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? 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ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. 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All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '1147', 'name' => 'ac4C antibody', 'description' => '', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_ac4C_C15200252.pdf', 'slug' => 'datasheet-ac4c-c15200252', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 14:43:31', 'created' => '2021-12-23 14:43:14', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/ac4c-monoclonal-antibody' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '3208', 'antibody_id' => '648', 'name' => 'ac4C Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig1-rip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig2-elisa.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="row"> <div class="small-6 columns"> <p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.</p> </div> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200252', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:22:01', 'created' => '2021-12-23 14:21:52' ) ) $pro = array( 'id' => '3209', 'antibody_id' => '648', 'name' => 'ac4C Antibody (sample size)', 'description' => '<p><span>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200252-10', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000582', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody-10ul', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:27:10', 'created' => '2022-01-05 10:27:10', 'ProductsGroup' => array( 'id' => '401', 'product_id' => '3209', 'group_id' => '351' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '3208', 'antibody_id' => '648', 'name' => 'ac4C Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig1-rip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig2-elisa.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="row"> <div class="small-6 columns"> <p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.</p> </div> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200252', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:22:01', 'created' => '2021-12-23 14:21:52' ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'ProductsApplication' => array( 'id' => '5498', 'product_id' => '3209', 'application_id' => '36' ) ) $slugs = array( (int) 0 => 'rip' ) $applications = array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'locale' => 'eng' ) $description = '<p>RIP</p>' $name = 'RIP' $document = array( 'id' => '1147', 'name' => 'ac4C antibody', 'description' => '', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_ac4C_C15200252.pdf', 'slug' => 'datasheet-ac4c-c15200252', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 14:43:31', 'created' => '2021-12-23 14:43:14', 'ProductsDocument' => array( 'id' => '3180', 'product_id' => '3209', 'document_id' => '1147' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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It’s a highly conserved RNA modification which is present in mRNA, tRNA and rRNA. ac4C has been shown to have important regulatory functions and is involved in the regulation of mRNA stability and in RNA processing. Moreover, ac4C promotes translation efficiency. ', 'clonality' => '', 'isotype' => ' IgG1', 'lot' => '001', 'concentration' => '0.7 µg/µl', 'reactivity' => 'Human, other (wide range): positive.', 'type' => 'Monoclonal', 'purity' => 'Protein G purified monoclonal antibody', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP*</td> <td>2 µg per IP</td> <td>Fig 1</td> </tr> <tr> <td>ELISA</td> <td>1:1,000</td> <td>Fig 2</td> </tr> </tbody> </table> <p><small><sup>*</sup>Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% sodium azide', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 14:22:41', 'created' => '2021-12-23 14:19:14', 'select_label' => '648 - ac4C monoclonal antibody (001 - 0.7 µg/µl - Human, other (wide range): positive. - Protein G purified monoclonal antibody - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/en/p/ac4c-monoclonal-antibody' ) $language = 'en' $meta_keywords = '' $meta_description = '' $meta_title = '' $product = array( 'Product' => array( 'id' => '3209', 'antibody_id' => '648', 'name' => 'ac4C Antibody (sample size)', 'description' => '<p><span>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig1-rip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig2-elisa.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="row"> <div class="small-6 columns"> <p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.</p> </div> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200252-10', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000582', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody-10ul', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:27:10', 'created' => '2022-01-05 10:27:10', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '648', 'name' => 'ac4C monoclonal antibody', 'description' => 'N4-acetylcytidine (ac4C) is the only acetylation that has been described in eukaryotic RNA. It’s a highly conserved RNA modification which is present in mRNA, tRNA and rRNA. ac4C has been shown to have important regulatory functions and is involved in the regulation of mRNA stability and in RNA processing. Moreover, ac4C promotes translation efficiency. ', 'clonality' => '', 'isotype' => ' IgG1', 'lot' => '001', 'concentration' => '0.7 µg/µl', 'reactivity' => 'Human, other (wide range): positive.', 'type' => 'Monoclonal', 'purity' => 'Protein G purified monoclonal antibody', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP*</td> <td>2 µg per IP</td> <td>Fig 1</td> </tr> <tr> <td>ELISA</td> <td>1:1,000</td> <td>Fig 2</td> </tr> </tbody> </table> <p><small><sup>*</sup>Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% sodium azide', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 14:22:41', 'created' => '2021-12-23 14:19:14', 'select_label' => '648 - ac4C monoclonal antibody (001 - 0.7 µg/µl - Human, other (wide range): positive. - Protein G purified monoclonal antibody - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '351', 'name' => 'C15200252', 'product_id' => '3208', 'modified' => '2022-01-05 10:27:41', 'created' => '2022-01-05 10:27:41' ), 'Master' => array( 'id' => '3208', 'antibody_id' => '648', 'name' => 'ac4C Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig1-rip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig2-elisa.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="row"> <div class="small-6 columns"> <p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.</p> </div> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200252', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:22:01', 'created' => '2021-12-23 14:21:52' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '109', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA modifications', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'rna-modifications-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody', 'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies', 'meta_title' => 'RNA modifications antibodies | Diagenode.com', 'modified' => '2020-09-01 11:43:28', 'created' => '2016-02-15 10:58:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '1147', 'name' => 'ac4C antibody', 'description' => '', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_ac4C_C15200252.pdf', 'slug' => 'datasheet-ac4c-c15200252', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 14:43:31', 'created' => '2021-12-23 14:43:14', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/ac4c-monoclonal-antibody' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '3208', 'antibody_id' => '648', 'name' => 'ac4C Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig1-rip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig2-elisa.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="row"> <div class="small-6 columns"> <p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.</p> </div> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200252', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:22:01', 'created' => '2021-12-23 14:21:52' ) ) $pro = array( 'id' => '3209', 'antibody_id' => '648', 'name' => 'ac4C Antibody (sample size)', 'description' => '<p><span>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200252-10', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000582', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody-10ul', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:27:10', 'created' => '2022-01-05 10:27:10', 'ProductsGroup' => array( 'id' => '401', 'product_id' => '3209', 'group_id' => '351' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '3208', 'antibody_id' => '648', 'name' => 'ac4C Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig1-rip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200252-fig2-elisa.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="row"> <div class="small-6 columns"> <p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.</p> </div> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200252', 'old_catalog_number' => '', 'sf_code' => 'C15200252-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ac4c-monoclonal-antibody', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 10:22:01', 'created' => '2021-12-23 14:21:52' ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'ProductsApplication' => array( 'id' => '5498', 'product_id' => '3209', 'application_id' => '36' ) ) $slugs = array( (int) 0 => 'rip' ) $applications = array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'locale' => 'eng' ) $description = '<p>RIP</p>' $name = 'RIP' $document = array( 'id' => '1147', 'name' => 'ac4C antibody', 'description' => '', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_ac4C_C15200252.pdf', 'slug' => 'datasheet-ac4c-c15200252', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 14:43:31', 'created' => '2021-12-23 14:43:14', 'ProductsDocument' => array( 'id' => '3180', 'product_id' => '3209', 'document_id' => '1147' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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