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<p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.</p>
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<p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p><strong>Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C</strong><br />To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.</p>
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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