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CRISPR/Cas9 C-terminal Antibody (sample size)

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

Antibody portal

Antibodies

Catalog Number

Format

Price

C15200223-14

14 µg

$130.00
Bulk order

Other format

C15200223-100

100 μg

C15200223-50

50 µg

Monoclonal antibody raised in mouse against the C-terminus of Cas9 nuclease (CRISPR-associated protein 9)

Lot	001
Concentration	2.2 µg/µl
Species reactivity	Streptococcus pyogenes
Type	Monoclonal
Purity	Protein G purified monoclonal antibody.
Host	Mouse
Storage Conditions	Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles
Storage Buffer	PBS containing 0.05 % Na-azide.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
Western Blotting	1:1,000 - 1:5,000	Fig 1,2
IP	13 µg/IP	Fig 3
IF	1:200	Fig 4

Validation data

Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9
Western blot was performed on protein extracts from HeLa cells transfected with Cas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15200223). The antibody was used at different dilutions. The marker is shown on the left, position of the Cas9 protein is indicated on the right.

Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9
Western blot was performed on 20 μg protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9 (cat. No. C15200223). The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right.

Figure 3. IP using the Diagenode monoclonal antibody directed against Cas9
IP was performed on whole cell extracts (250 μg) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 13 μg of the Diagenode antibody against Cas9 (cat. No. C15200223). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (15 μg) is shown in lane 1 and 2.

Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (cat. No. C15200223) diluted 1:200, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488. Nuclei were counter-stained with Hoechst 33342. Figure 4 shows the result in the presence (left) or absence (right) of doxycycline.
Background
A breakthrough in genome engineering! The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate up- or downregulation of specific endogenous genes or to alter histone modifications or DNA methylation.

Applications

WB
Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more

IF
Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more

IP
Immunoprecipitation Read more

Documents
Datasheet C15200223 CRISPR Cas9 Csn1 monoclonal antibody DATASHEET
Datasheet description Download

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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15200223). The antibody was used at different dilutions. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<p><small><strong> Figure 3. IP using the Diagenode monoclonal antibody directed against Cas9 </strong><br />IP was performed on whole cell extracts (250 &mu;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 13 &mu;g of the Diagenode antibody against Cas9 (cat. No. C15200223). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (15 &mu;g) is shown in lane 1 and 2. </small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on 20 &micro;g protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9 (cat. No. C15200223). The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IP.png" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 3. IP using the Diagenode monoclonal antibody directed against Cas9 </strong><br />IP was performed on whole cell extracts (250 &amp; micro;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 13 &micro;g of the Diagenode antibody against Cas9 (cat. No. C15200223). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (15 &micro;g) is shown in lane 1 and 2. </small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IF.png" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (cat. No. C15200223) diluted 1:200, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488. Nuclei were counter-stained with Hoechst 33342. Figure 4 shows the result in the presence (left) or absence (right) of doxycycline. </small></p>
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			'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody   validated  in  IP, IF and WB. Batch-specific data available on the website. Alternative name: Csn1. Sample size available.',
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			'name' => 'CRISPR/Cas9 Antibody',
			'description' => '<p>Polyclonal antibody raised in rabbit against the <strong>Cas9</strong> nuclease (<strong>CRISPR</strong>-associated protein 9) using a recombinant protein.&nbsp;</p>',
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-fig1.png" /></center></div>
<div class="small-6 columns">
<p><strong>Figure 1. ChIP using the Diagenode antibody directed against Cas9</strong><br />ChIP was performed on sheared chromatin from 4 million HEK293T cells stably expressing nuclease dead Cas9 and sgRNA targeting a sequence in intron 8 of the GAPDH gene, using the iDeal ChIP-seq kit for transcription factors. A titration consisting of 1, 2, 5 and 10 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258) was tested. IgG (2 &micro;g/IP) was used as negative IP control. qPCR was performed with primers specific for the targeted sequence in the GAPDH gene, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="row">
<div class="small-12 columns"><center>A.<img src="https://www.diagenode.com/img/product/antibodies/C15310258-chipseq-a.jpg" width="700" /></center><center>B.<img src="https://www.diagenode.com/img/product/antibodies/C15310258-chipseq-b.jpg" width="700" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against Cas9</strong><br />ChIP was performed on sheared chromatin from 4 million HEK293T cells stably expressing dCas9 and a GAPDH sgRNA cells using 2 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the ChIP-seq profile in a region of chromosome 12 surrounding the GAPDH gene (fig 2B) and in a region of chromosome 2 surrounding an off-target peak in the YIPF4 gene.</p>
</div>
</div>
<div class="row">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-WB.png" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with dCas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310258). The antibody was diluted 1:5,000. The marker is shown on the left, the position of the Cas9 protein is indicated on the right.</p>
</div>
</div>
<div class="row">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-IP.png" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. IP using the Diagenode antibody directed against Cas9</strong><br />IP was performed on whole cell extracts (500 &micro;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (25 &micro;g) is shown in lane 1 and 2.</p>
</div>
</div>
<div class="row">
<div class="small-12 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-IF.png" width="500" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against Cas9</strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 antibody (cat. No. C15310258) diluted 1:1000, followed by incubation with a goat anti-rabbit secondary antibody coupled to AF594. Nuclei were counter-stained with Hoechst 33342. Figure 5 shows the result in the presence (left) or absence (right) of doxycycline.</p>
</div>
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			'info2' => '<p style="text-align: justify;">CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the <strong>CRISPR/Cas9</strong> (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
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			'meta_description' => 'S. pyogenes CRISPR/Cas9 Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, IP, IF and WB. Batch-specific data available on the website. Sample size available.',
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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			'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
			'meta_title' => 'Sample-size Antibodies | Diagenode',
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			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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			'name' => '<em>S. pyogenes</EM> CRISPR/Cas9 antibodies',
			'description' => '<p>Diagenode offers the broad range of antibodies raised against the N- or C-terminus of the Cas9 nuclease from <em>Streptococcus <g class="gr_ gr_5 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="5" data-gr-id="5">pyogenes</g></em>. These highly specific polyclonal and monoclonal antibodies are validated in Western blot, immunoprecipitation, immunofluorescence and in chromatin immunoprecipitation.</p>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h2><em><a name="pyogenes"></a>S. pyogenes</em> CRISPR/Cas9 antibodies<a></a></h2>
<div class="panel">
<h2>Discover our first monoclonal CRISPR/Cas9 antibody validated in ChIP<br /><br /></h2>
<div class="row">
<div class="small-5 medium-5 large-5 columns"><img src="/img/landing-pages/crispr-cas9-chip-on-hih3t3.jpg" alt="" /></div>
<div class="small-7 medium-7 large-7 columns">
<ul>
<li>Validated in chromatin immunoprecipitation</li>
<li>Performs better than FLAG antibody</li>
<li>Excellent for WB, IF and IP</li>
</ul>
<p><small><strong>ChIP</strong> was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 &micro;g chromatin was incubated overnight at 4&deg;C with 5 or 10 &micro;g of either an anti-FLAG antibody or the Diagenode antibody against Cas9 (Cat. No. C15200229). Mouse IgG was used as a negative IP control. qPCR was performed with primers specific for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns text-right"><a href="/p/crispr-cas9-monoclonal-antibody-50-ug-25-&mu;l" class="tiny details button radius">Learn more</a></div>
</div>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>First ChIP-grade CRISPR/Cas9 polyclonal antibody</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/landing-pages/c_a_s9-chip-grade-antibody.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Excellent polyclonal antibody for chromatin immunoprecipitation</li>
<li>Optimized for highest ChIP specificity and yields</li>
<li>Validated for all applications including immunoblotting, immunofluorescence and western blot</li>
</ul>
<p><small><strong>ChIP</strong> was performed on NIH3T3 cells stably expressing GFP- H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 &mu;g chromatin was incubated with either 5 &mu;g of an anti-FLAG antibody or 2 &mu;l of the Diagenode antibody against Cas9. The pre-immune serum (PPI) was used as negative IP control. Then qPCR was performed with primers specific for the GFP gene, and for two non-targeted regions: Ppap2c and Prkcd, used as negative controls. This figure shows the recovery, expressed as a % of input.</small></p>
<p class="text-right"><a href="../p/crispr-cas9-polyclonal-antibody" class="details tiny button">Learn more</a></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>CRISPR/Cas9 monoclonal antibody 4G10</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/landing-pages/cas9_4g10_fig1.png" width="170" height="302" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Antibody raised against N-terminus of Cas9 nuclease</li>
<li>Validated for western blot, IP and immunofluorescence</li>
</ul>
<p><small><strong>Immunofluorescence</strong>: Hela cells were transiently transfected with a Cas9 expression vector. The cells were fixed in 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA. The cells were stained with the Cas9 antibody at 4&deg;C o/n, followed by incubation with an anti mouse secondary antibody coupled to AF488 for 1 h at RT. Nuclei were counter-stained with Hoechst 33342 (right).</small></p>
<p class="text-right"><a href="../p/crispr-cas9-monoclonal-antibody-4g10-50-ug" class="details tiny button">Learn more</a></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>CRISPR/Cas9 C-terminal monoclonal antibody</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IP.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Antibody raised against C-terminus of Cas9 nuclease</li>
<li>Validated for western blot, IP and immunofluorescence</li>
</ul>
<p><small><strong> Western blot</strong> was performed on 20 &mu;g protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9. The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
<p class="text-right"><a href="../p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug" class="details tiny button">Learn more</a></p>
</div>
<div class="small-12 medium-12 large-12 columns">
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<a name="table"></a>
<table>
<thead>
<tr>
<th>Antibody</th>
<th>WB</th>
<th>IF</th>
<th>IP</th>
<th>ChIP</th>
<th>Antibody raised against</th>
</tr>
</thead>
<tbody>
<tr>
<td><a href="../p/crispr-cas9-monoclonal-antibody-50-ug-25-&mu;l"><strong class="diacol">CRISPR/Cas9 monoclonal antibody</strong></a></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><span class="diacol">N-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-polyclonal-antibody">CRISPR/Cas9 polyclonal antibody</a></td>
<td>++</td>
<td>++</td>
<td>++</td>
<td><strong class="diacol">+++</strong></td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-monoclonal-antibody-4g10-50-ug">CRISPR/Cas9 monoclonal antibody 4G10</a></td>
<td>+++</td>
<td>+++</td>
<td>++</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="../p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul"><strong class="diacol">CRISPR/Cas9 C-terminal monoclonal antibody</strong></a> <span class="label alert" style="font-size: 0.9rem;">NEW!</span></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">no</strong></td>
<td><strong class="diacol">+</strong></td>
<td><span class="diacol">C-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug">CRISPR/Cas9 C-terminal monoclonal antibody</a></td>
<td>++</td>
<td>++</td>
<td>+</td>
<td>no</td>
<td><span class="diacol">C-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-monoclonal-antibody-7A9-50-mg">CRISPR/Cas9 monoclonal antibody 7A9</a></td>
<td>++</td>
<td>++</td>
<td>++</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-hrp-monoclonal-antibody-50-ul">CRISPR/Cas9 - HRP monoclonal antibody 7A9</a></td>
<td>+++</td>
<td>no</td>
<td>no</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15200223). The antibody was used at different dilutions. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on 20 &micro;g protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9 (cat. No. C15200223). The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<p><small><strong> Figure 3. IP using the Diagenode monoclonal antibody directed against Cas9 </strong><br />IP was performed on whole cell extracts (250 &amp; micro;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 13 &micro;g of the Diagenode antibody against Cas9 (cat. No. C15200223). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (15 &micro;g) is shown in lane 1 and 2. </small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (cat. No. C15200223) diluted 1:200, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488. Nuclei were counter-stained with Hoechst 33342. Figure 4 shows the result in the presence (left) or absence (right) of doxycycline. </small></p>
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            <h6 style="height:60px">CRISPR/Cas9 Antibody</h6>
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	'description' => '<p>Polyclonal antibody raised in rabbit against the <strong>Cas9</strong> nuclease (<strong>CRISPR</strong>-associated protein 9) using a recombinant protein.&nbsp;</p>',
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-fig1.png" /></center></div>
<div class="small-6 columns">
<p><strong>Figure 1. ChIP using the Diagenode antibody directed against Cas9</strong><br />ChIP was performed on sheared chromatin from 4 million HEK293T cells stably expressing nuclease dead Cas9 and sgRNA targeting a sequence in intron 8 of the GAPDH gene, using the iDeal ChIP-seq kit for transcription factors. A titration consisting of 1, 2, 5 and 10 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258) was tested. IgG (2 &micro;g/IP) was used as negative IP control. qPCR was performed with primers specific for the targeted sequence in the GAPDH gene, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="row">
<div class="small-12 columns"><center>A.<img src="https://www.diagenode.com/img/product/antibodies/C15310258-chipseq-a.jpg" width="700" /></center><center>B.<img src="https://www.diagenode.com/img/product/antibodies/C15310258-chipseq-b.jpg" width="700" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against Cas9</strong><br />ChIP was performed on sheared chromatin from 4 million HEK293T cells stably expressing dCas9 and a GAPDH sgRNA cells using 2 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the ChIP-seq profile in a region of chromosome 12 surrounding the GAPDH gene (fig 2B) and in a region of chromosome 2 surrounding an off-target peak in the YIPF4 gene.</p>
</div>
</div>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-WB.png" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with dCas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310258). The antibody was diluted 1:5,000. The marker is shown on the left, the position of the Cas9 protein is indicated on the right.</p>
</div>
</div>
<div class="row">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-IP.png" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. IP using the Diagenode antibody directed against Cas9</strong><br />IP was performed on whole cell extracts (500 &micro;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (25 &micro;g) is shown in lane 1 and 2.</p>
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<div class="small-12 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-IF.png" width="500" /></center></div>
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<p><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against Cas9</strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 antibody (cat. No. C15310258) diluted 1:1000, followed by incubation with a goat anti-rabbit secondary antibody coupled to AF594. Nuclei were counter-stained with Hoechst 33342. Figure 5 shows the result in the presence (left) or absence (right) of doxycycline.</p>
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	'price_GBP' => '500',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
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	'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody  validated  in  WB, IP and IF. Batch-specific data available on the website. Sample size available',
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			'name' => 'CRISPR/Cas9 C-terminal Antibody (sample size)',
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			'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15200223). The antibody was used at different dilutions. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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</div>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-WB2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on 20 &mu;g protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9 (cat. No. C15200223). The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IP.png" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. IP using the Diagenode monoclonal antibody directed against Cas9 </strong><br />IP was performed on whole cell extracts (250 &mu;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 13 &mu;g of the Diagenode antibody against Cas9 (cat. No. C15200223). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (15 &mu;g) is shown in lane 1 and 2. </small></p>
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<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IF.png" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small><strong> Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (cat. No. C15200223) diluted 1:200, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488. Nuclei were counter-stained with Hoechst 33342. Figure 4 shows the result in the presence (left) or absence (right) of doxycycline. </small></p>
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			'info2' => '<p>A breakthrough in genome engineering! The CRISPR/Cas9 (<strong>C</strong>RISPR-<strong>as</strong>sociated protein <strong>9</strong> nuclease) system uses a RNA-guided endonuclease technology which allows&nbsp;for inducing indel mutations, specific sequence replacements or insertions and large&nbsp;deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate up- or downregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
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<th>References</th>
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<td>Fig 1,2</td>
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<tr>
<td>IP</td>
<td>13 &micro;g/IP</td>
<td>Fig 3</td>
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<tr>
<td>IF</td>
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<td>Fig 4</td>
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<p></p>',
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<div class="small-4 columns">
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<div class="small-8 columns">
<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15200223). The antibody was used at different dilutions. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<div class="row">
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<div class="small-8 columns">
<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on 20 &mu;g protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9 (cat. No. C15200223). The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IP.png" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. IP using the Diagenode monoclonal antibody directed against Cas9 </strong><br />IP was performed on whole cell extracts (250 &mu;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 13 &mu;g of the Diagenode antibody against Cas9 (cat. No. C15200223). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (15 &mu;g) is shown in lane 1 and 2. </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IF.png" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (cat. No. C15200223) diluted 1:200, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488. Nuclei were counter-stained with Hoechst 33342. Figure 4 shows the result in the presence (left) or absence (right) of doxycycline. </small></p>
</div>
</div>',
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		'info2' => '<p>A breakthrough in genome engineering! The CRISPR/Cas9 (<strong>C</strong>RISPR-<strong>as</strong>sociated protein <strong>9</strong> nuclease) system uses a RNA-guided endonuclease technology which allows&nbsp;for inducing indel mutations, specific sequence replacements or insertions and large&nbsp;deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate up- or downregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
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		'price_EUR' => '125',
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		'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-14-ug-sample-size',
		'meta_title' => 'CRISPR/Cas9 C-terminal Monoclonal Antibody | Diagenode',
		'meta_keywords' => 'CRISPR/Cas9 C-terminal monoclonal antibody, sample size',
		'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody  validated  in  WB, IP and IF. Batch-specific data available on the website. Sample size available',
		'modified' => '2022-01-05 15:11:04',
		'created' => '2015-10-23 15:36:11',
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	'Antibody' => array(
		'host' => '*****',
		'id' => '445',
		'name' => 'CRISPR/Cas9 C-terminal monoclonal antibody',
		'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.',
		'clonality' => '',
		'isotype' => 'IgG1',
		'lot' => '001',
		'concentration' => '2.2 µg/µl',
		'reactivity' => 'Streptococcus pyogenes',
		'type' => 'Monoclonal',
		'purity' => 'Protein G purified monoclonal antibody.',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:1,000 - 1:5,000</td>
<td>Fig 1,2</td>
</tr>
<tr>
<td>IP</td>
<td>13 &micro;g/IP</td>
<td>Fig 3</td>
</tr>
<tr>
<td>IF</td>
<td>1:200</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p></p>',
		'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles',
		'storage_buffer' => 'PBS containing 0.05 % Na-azide.',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => 'Q99ZW2',
		'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-50-ug',
		'meta_keywords' => 'CRISPR/Cas9 C-terminal monoclonal antibody',
		'meta_description' => 'CRISPR/Cas9 C-terminal monoclonal antibody',
		'modified' => '2018-08-30 11:15:07',
		'created' => '2015-08-26 16:19:09',
		'select_label' => '445 - CRISPR/Cas9 C-terminal monoclonal antibody (001 - 2.2 µg/µl - Streptococcus pyogenes - Protein G purified monoclonal antibody. - Mouse)'
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			'created' => '2016-02-18 18:00:45'
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			'name' => 'CRISPR/Cas9 C-terminal Antibody',
			'description' => '<p>Alternative name: <strong>Csn1</strong></p>
<p>Monoclonal antibody raised in mouse against the C-terminus of Cas9 nuclease (CRISPR-associated protein 9)</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15200223). The antibody was used at different dilutions. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-WB2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on 20 &micro;g protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9 (cat. No. C15200223). The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IP.png" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 3. IP using the Diagenode monoclonal antibody directed against Cas9 </strong><br />IP was performed on whole cell extracts (250 &amp; micro;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 13 &micro;g of the Diagenode antibody against Cas9 (cat. No. C15200223). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (15 &micro;g) is shown in lane 1 and 2. </small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IF.png" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (cat. No. C15200223) diluted 1:200, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488. Nuclei were counter-stained with Hoechst 33342. Figure 4 shows the result in the presence (left) or absence (right) of doxycycline. </small></p>
</div>
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			'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-100-ug',
			'meta_title' => 'CRISPR/Cas9 C-terminal Monoclonal Antibody | Diagenode',
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			'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody   validated  in  IP, IF and WB. Batch-specific data available on the website. Alternative name: Csn1. Sample size available.',
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			'id' => '2687',
			'antibody_id' => '446',
			'name' => 'CRISPR/Cas9 Antibody',
			'description' => '<p>Polyclonal antibody raised in rabbit against the <strong>Cas9</strong> nuclease (<strong>CRISPR</strong>-associated protein 9) using a recombinant protein.&nbsp;</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-fig1.png" /></center></div>
<div class="small-6 columns">
<p><strong>Figure 1. ChIP using the Diagenode antibody directed against Cas9</strong><br />ChIP was performed on sheared chromatin from 4 million HEK293T cells stably expressing nuclease dead Cas9 and sgRNA targeting a sequence in intron 8 of the GAPDH gene, using the iDeal ChIP-seq kit for transcription factors. A titration consisting of 1, 2, 5 and 10 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258) was tested. IgG (2 &micro;g/IP) was used as negative IP control. qPCR was performed with primers specific for the targeted sequence in the GAPDH gene, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
</div>
</div>
<div class="row">
<div class="small-12 columns"><center>A.<img src="https://www.diagenode.com/img/product/antibodies/C15310258-chipseq-a.jpg" width="700" /></center><center>B.<img src="https://www.diagenode.com/img/product/antibodies/C15310258-chipseq-b.jpg" width="700" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against Cas9</strong><br />ChIP was performed on sheared chromatin from 4 million HEK293T cells stably expressing dCas9 and a GAPDH sgRNA cells using 2 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the ChIP-seq profile in a region of chromosome 12 surrounding the GAPDH gene (fig 2B) and in a region of chromosome 2 surrounding an off-target peak in the YIPF4 gene.</p>
</div>
</div>
<div class="row">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-WB.png" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with dCas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310258). The antibody was diluted 1:5,000. The marker is shown on the left, the position of the Cas9 protein is indicated on the right.</p>
</div>
</div>
<div class="row">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-IP.png" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. IP using the Diagenode antibody directed against Cas9</strong><br />IP was performed on whole cell extracts (500 &micro;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (25 &micro;g) is shown in lane 1 and 2.</p>
</div>
</div>
<div class="row">
<div class="small-12 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-IF.png" width="500" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against Cas9</strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 antibody (cat. No. C15310258) diluted 1:1000, followed by incubation with a goat anti-rabbit secondary antibody coupled to AF594. Nuclei were counter-stained with Hoechst 33342. Figure 5 shows the result in the presence (left) or absence (right) of doxycycline.</p>
</div>
</div>',
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			'info2' => '<p style="text-align: justify;">CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the <strong>CRISPR/Cas9</strong> (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
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			'meta_title' => 'CRISPR/Cas9 Antibody - ChIP-seq Grade (C15310258) | Diagenode',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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			'name' => '<em>S. pyogenes</EM> CRISPR/Cas9 antibodies',
			'description' => '<p>Diagenode offers the broad range of antibodies raised against the N- or C-terminus of the Cas9 nuclease from <em>Streptococcus <g class="gr_ gr_5 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="5" data-gr-id="5">pyogenes</g></em>. These highly specific polyclonal and monoclonal antibodies are validated in Western blot, immunoprecipitation, immunofluorescence and in chromatin immunoprecipitation.</p>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h2><em><a name="pyogenes"></a>S. pyogenes</em> CRISPR/Cas9 antibodies<a></a></h2>
<div class="panel">
<h2>Discover our first monoclonal CRISPR/Cas9 antibody validated in ChIP<br /><br /></h2>
<div class="row">
<div class="small-5 medium-5 large-5 columns"><img src="/img/landing-pages/crispr-cas9-chip-on-hih3t3.jpg" alt="" /></div>
<div class="small-7 medium-7 large-7 columns">
<ul>
<li>Validated in chromatin immunoprecipitation</li>
<li>Performs better than FLAG antibody</li>
<li>Excellent for WB, IF and IP</li>
</ul>
<p><small><strong>ChIP</strong> was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 &micro;g chromatin was incubated overnight at 4&deg;C with 5 or 10 &micro;g of either an anti-FLAG antibody or the Diagenode antibody against Cas9 (Cat. No. C15200229). Mouse IgG was used as a negative IP control. qPCR was performed with primers specific for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns text-right"><a href="/p/crispr-cas9-monoclonal-antibody-50-ug-25-&mu;l" class="tiny details button radius">Learn more</a></div>
</div>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>First ChIP-grade CRISPR/Cas9 polyclonal antibody</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/landing-pages/c_a_s9-chip-grade-antibody.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Excellent polyclonal antibody for chromatin immunoprecipitation</li>
<li>Optimized for highest ChIP specificity and yields</li>
<li>Validated for all applications including immunoblotting, immunofluorescence and western blot</li>
</ul>
<p><small><strong>ChIP</strong> was performed on NIH3T3 cells stably expressing GFP- H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 &mu;g chromatin was incubated with either 5 &mu;g of an anti-FLAG antibody or 2 &mu;l of the Diagenode antibody against Cas9. The pre-immune serum (PPI) was used as negative IP control. Then qPCR was performed with primers specific for the GFP gene, and for two non-targeted regions: Ppap2c and Prkcd, used as negative controls. This figure shows the recovery, expressed as a % of input.</small></p>
<p class="text-right"><a href="../p/crispr-cas9-polyclonal-antibody" class="details tiny button">Learn more</a></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>CRISPR/Cas9 monoclonal antibody 4G10</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/landing-pages/cas9_4g10_fig1.png" width="170" height="302" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Antibody raised against N-terminus of Cas9 nuclease</li>
<li>Validated for western blot, IP and immunofluorescence</li>
</ul>
<p><small><strong>Immunofluorescence</strong>: Hela cells were transiently transfected with a Cas9 expression vector. The cells were fixed in 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA. The cells were stained with the Cas9 antibody at 4&deg;C o/n, followed by incubation with an anti mouse secondary antibody coupled to AF488 for 1 h at RT. Nuclei were counter-stained with Hoechst 33342 (right).</small></p>
<p class="text-right"><a href="../p/crispr-cas9-monoclonal-antibody-4g10-50-ug" class="details tiny button">Learn more</a></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>CRISPR/Cas9 C-terminal monoclonal antibody</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IP.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Antibody raised against C-terminus of Cas9 nuclease</li>
<li>Validated for western blot, IP and immunofluorescence</li>
</ul>
<p><small><strong> Western blot</strong> was performed on 20 &mu;g protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9. The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
<p class="text-right"><a href="../p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug" class="details tiny button">Learn more</a></p>
</div>
<div class="small-12 medium-12 large-12 columns">
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<a name="table"></a>
<table>
<thead>
<tr>
<th>Antibody</th>
<th>WB</th>
<th>IF</th>
<th>IP</th>
<th>ChIP</th>
<th>Antibody raised against</th>
</tr>
</thead>
<tbody>
<tr>
<td><a href="../p/crispr-cas9-monoclonal-antibody-50-ug-25-&mu;l"><strong class="diacol">CRISPR/Cas9 monoclonal antibody</strong></a></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><span class="diacol">N-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-polyclonal-antibody">CRISPR/Cas9 polyclonal antibody</a></td>
<td>++</td>
<td>++</td>
<td>++</td>
<td><strong class="diacol">+++</strong></td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-monoclonal-antibody-4g10-50-ug">CRISPR/Cas9 monoclonal antibody 4G10</a></td>
<td>+++</td>
<td>+++</td>
<td>++</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="../p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul"><strong class="diacol">CRISPR/Cas9 C-terminal monoclonal antibody</strong></a> <span class="label alert" style="font-size: 0.9rem;">NEW!</span></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">no</strong></td>
<td><strong class="diacol">+</strong></td>
<td><span class="diacol">C-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug">CRISPR/Cas9 C-terminal monoclonal antibody</a></td>
<td>++</td>
<td>++</td>
<td>+</td>
<td>no</td>
<td><span class="diacol">C-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-monoclonal-antibody-7A9-50-mg">CRISPR/Cas9 monoclonal antibody 7A9</a></td>
<td>++</td>
<td>++</td>
<td>++</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-hrp-monoclonal-antibody-50-ul">CRISPR/Cas9 - HRP monoclonal antibody 7A9</a></td>
<td>+++</td>
<td>no</td>
<td>no</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
</tbody>
</table>
</div>
</div>
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15200223). The antibody was used at different dilutions. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on 20 &micro;g protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9 (cat. No. C15200223). The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<div class="small-7 columns">
<p><small><strong> Figure 3. IP using the Diagenode monoclonal antibody directed against Cas9 </strong><br />IP was performed on whole cell extracts (250 &amp; micro;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 13 &micro;g of the Diagenode antibody against Cas9 (cat. No. C15200223). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (15 &micro;g) is shown in lane 1 and 2. </small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (cat. No. C15200223) diluted 1:200, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488. Nuclei were counter-stained with Hoechst 33342. Figure 4 shows the result in the presence (left) or absence (right) of doxycycline. </small></p>
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	'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated  in WB, IP and IF. Batch-specific data available on the website. Alternative name: Csn1. Sample size available.',
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    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/crispr-cas9-polyclonal-antibody"><img src="/img/product/antibodies/cas9-icon.png" alt="CRISPR/Cas9 Antibody " class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C15310258-100</span>
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                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2687" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> CRISPR/Cas9 Antibody</strong>  to my shopping cart.</p>

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                <button class="alert small button expand" onclick="$(this).addToCart('CRISPR/Cas9 Antibody',
                    'C15310258-100',
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                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
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</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="crispr-cas9-polyclonal-antibody" data-reveal-id="cartModal-2687" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">CRISPR/Cas9 Antibody</h6>
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'
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	'id' => '2687',
	'antibody_id' => '446',
	'name' => 'CRISPR/Cas9 Antibody',
	'description' => '<p>Polyclonal antibody raised in rabbit against the <strong>Cas9</strong> nuclease (<strong>CRISPR</strong>-associated protein 9) using a recombinant protein.&nbsp;</p>',
	'label1' => 'Validation data',
	'info1' => '<div class="row">
<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-fig1.png" /></center></div>
<div class="small-6 columns">
<p><strong>Figure 1. ChIP using the Diagenode antibody directed against Cas9</strong><br />ChIP was performed on sheared chromatin from 4 million HEK293T cells stably expressing nuclease dead Cas9 and sgRNA targeting a sequence in intron 8 of the GAPDH gene, using the iDeal ChIP-seq kit for transcription factors. A titration consisting of 1, 2, 5 and 10 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258) was tested. IgG (2 &micro;g/IP) was used as negative IP control. qPCR was performed with primers specific for the targeted sequence in the GAPDH gene, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
</div>
</div>
<div class="row">
<div class="small-12 columns"><center>A.<img src="https://www.diagenode.com/img/product/antibodies/C15310258-chipseq-a.jpg" width="700" /></center><center>B.<img src="https://www.diagenode.com/img/product/antibodies/C15310258-chipseq-b.jpg" width="700" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against Cas9</strong><br />ChIP was performed on sheared chromatin from 4 million HEK293T cells stably expressing dCas9 and a GAPDH sgRNA cells using 2 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the ChIP-seq profile in a region of chromosome 12 surrounding the GAPDH gene (fig 2B) and in a region of chromosome 2 surrounding an off-target peak in the YIPF4 gene.</p>
</div>
</div>
<div class="row">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-WB.png" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with dCas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310258). The antibody was diluted 1:5,000. The marker is shown on the left, the position of the Cas9 protein is indicated on the right.</p>
</div>
</div>
<div class="row">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-IP.png" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. IP using the Diagenode antibody directed against Cas9</strong><br />IP was performed on whole cell extracts (500 &micro;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (25 &micro;g) is shown in lane 1 and 2.</p>
</div>
</div>
<div class="row">
<div class="small-12 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-IF.png" width="500" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against Cas9</strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 antibody (cat. No. C15310258) diluted 1:1000, followed by incubation with a goat anti-rabbit secondary antibody coupled to AF594. Nuclei were counter-stained with Hoechst 33342. Figure 5 shows the result in the presence (left) or absence (right) of doxycycline.</p>
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	'info2' => '<p style="text-align: justify;">CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the <strong>CRISPR/Cas9</strong> (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
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	'meta_description' => 'S. pyogenes CRISPR/Cas9 Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, IP, IF and WB. Batch-specific data available on the website. Sample size available.',
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	'created' => '2015-09-16 14:58:46',
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	'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
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	'created' => '2015-07-08 13:46:50',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'en',
	'meta_keywords' => 'CRISPR/Cas9 C-terminal monoclonal antibody, sample size',
	'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody  validated  in  WB, IP and IF. Batch-specific data available on the website. Sample size available',
	'meta_title' => 'CRISPR/Cas9 C-terminal Monoclonal Antibody | Diagenode',
	'product' => array(
		'Product' => array(
			'id' => '2764',
			'antibody_id' => '445',
			'name' => 'CRISPR/Cas9 C-terminal Antibody (sample size)',
			'description' => '<p>Monoclonal antibody raised in mouse against the C-terminus of Cas9 nuclease (CRISPR-associated protein 9)</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15200223). The antibody was used at different dilutions. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-WB2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on 20 &mu;g protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9 (cat. No. C15200223). The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IP.png" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. IP using the Diagenode monoclonal antibody directed against Cas9 </strong><br />IP was performed on whole cell extracts (250 &mu;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 13 &mu;g of the Diagenode antibody against Cas9 (cat. No. C15200223). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (15 &mu;g) is shown in lane 1 and 2. </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IF.png" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (cat. No. C15200223) diluted 1:200, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488. Nuclei were counter-stained with Hoechst 33342. Figure 4 shows the result in the presence (left) or absence (right) of doxycycline. </small></p>
</div>
</div>',
			'label2' => 'Background',
			'info2' => '<p>A breakthrough in genome engineering! The CRISPR/Cas9 (<strong>C</strong>RISPR-<strong>as</strong>sociated protein <strong>9</strong> nuclease) system uses a RNA-guided endonuclease technology which allows&nbsp;for inducing indel mutations, specific sequence replacements or insertions and large&nbsp;deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate up- or downregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '14 µg',
			'catalog_number' => 'C15200223-14',
			'old_catalog_number' => '',
			'sf_code' => 'C15200223-58210',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '125',
			'price_USD' => '130',
			'price_GBP' => '120',
			'price_JPY' => '19580',
			'price_CNY' => '0',
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			'except_countries' => 'None',
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			'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-14-ug-sample-size',
			'meta_title' => 'CRISPR/Cas9 C-terminal Monoclonal Antibody | Diagenode',
			'meta_keywords' => 'CRISPR/Cas9 C-terminal monoclonal antibody, sample size',
			'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody  validated  in  WB, IP and IF. Batch-specific data available on the website. Sample size available',
			'modified' => '2022-01-05 15:11:04',
			'created' => '2015-10-23 15:36:11',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '445',
			'name' => 'CRISPR/Cas9 C-terminal monoclonal antibody',
			'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.',
			'clonality' => '',
			'isotype' => 'IgG1',
			'lot' => '001',
			'concentration' => '2.2 µg/µl',
			'reactivity' => 'Streptococcus pyogenes',
			'type' => 'Monoclonal',
			'purity' => 'Protein G purified monoclonal antibody.',
			'classification' => '',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:1,000 - 1:5,000</td>
<td>Fig 1,2</td>
</tr>
<tr>
<td>IP</td>
<td>13 &micro;g/IP</td>
<td>Fig 3</td>
</tr>
<tr>
<td>IF</td>
<td>1:200</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p></p>',
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15200223). The antibody was used at different dilutions. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on 20 &mu;g protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9 (cat. No. C15200223). The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<p><small><strong> Figure 3. IP using the Diagenode monoclonal antibody directed against Cas9 </strong><br />IP was performed on whole cell extracts (250 &mu;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 13 &mu;g of the Diagenode antibody against Cas9 (cat. No. C15200223). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (15 &mu;g) is shown in lane 1 and 2. </small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (cat. No. C15200223) diluted 1:200, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488. Nuclei were counter-stained with Hoechst 33342. Figure 4 shows the result in the presence (left) or absence (right) of doxycycline. </small></p>
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15200223). The antibody was used at different dilutions. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-WB2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on 20 &micro;g protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9 (cat. No. C15200223). The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IP.png" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 3. IP using the Diagenode monoclonal antibody directed against Cas9 </strong><br />IP was performed on whole cell extracts (250 &amp; micro;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 13 &micro;g of the Diagenode antibody against Cas9 (cat. No. C15200223). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (15 &micro;g) is shown in lane 1 and 2. </small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (cat. No. C15200223) diluted 1:200, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488. Nuclei were counter-stained with Hoechst 33342. Figure 4 shows the result in the presence (left) or absence (right) of doxycycline. </small></p>
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			'name' => 'CRISPR/Cas9 Antibody',
			'description' => '<p>Polyclonal antibody raised in rabbit against the <strong>Cas9</strong> nuclease (<strong>CRISPR</strong>-associated protein 9) using a recombinant protein.&nbsp;</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-fig1.png" /></center></div>
<div class="small-6 columns">
<p><strong>Figure 1. ChIP using the Diagenode antibody directed against Cas9</strong><br />ChIP was performed on sheared chromatin from 4 million HEK293T cells stably expressing nuclease dead Cas9 and sgRNA targeting a sequence in intron 8 of the GAPDH gene, using the iDeal ChIP-seq kit for transcription factors. A titration consisting of 1, 2, 5 and 10 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258) was tested. IgG (2 &micro;g/IP) was used as negative IP control. qPCR was performed with primers specific for the targeted sequence in the GAPDH gene, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="row">
<div class="small-12 columns"><center>A.<img src="https://www.diagenode.com/img/product/antibodies/C15310258-chipseq-a.jpg" width="700" /></center><center>B.<img src="https://www.diagenode.com/img/product/antibodies/C15310258-chipseq-b.jpg" width="700" /></center></div>
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<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against Cas9</strong><br />ChIP was performed on sheared chromatin from 4 million HEK293T cells stably expressing dCas9 and a GAPDH sgRNA cells using 2 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the ChIP-seq profile in a region of chromosome 12 surrounding the GAPDH gene (fig 2B) and in a region of chromosome 2 surrounding an off-target peak in the YIPF4 gene.</p>
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<div class="row">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-WB.png" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with dCas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310258). The antibody was diluted 1:5,000. The marker is shown on the left, the position of the Cas9 protein is indicated on the right.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-IP.png" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. IP using the Diagenode antibody directed against Cas9</strong><br />IP was performed on whole cell extracts (500 &micro;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (25 &micro;g) is shown in lane 1 and 2.</p>
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<div class="row">
<div class="small-12 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-IF.png" width="500" /></center></div>
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<div class="row">
<div class="small-12 columns">
<p><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against Cas9</strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 antibody (cat. No. C15310258) diluted 1:1000, followed by incubation with a goat anti-rabbit secondary antibody coupled to AF594. Nuclei were counter-stained with Hoechst 33342. Figure 5 shows the result in the presence (left) or absence (right) of doxycycline.</p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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			'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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<li>Batch-specific data is available on the website</li>
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			'description' => '<p>Diagenode offers the broad range of antibodies raised against the N- or C-terminus of the Cas9 nuclease from <em>Streptococcus <g class="gr_ gr_5 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="5" data-gr-id="5">pyogenes</g></em>. These highly specific polyclonal and monoclonal antibodies are validated in Western blot, immunoprecipitation, immunofluorescence and in chromatin immunoprecipitation.</p>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h2><em><a name="pyogenes"></a>S. pyogenes</em> CRISPR/Cas9 antibodies<a></a></h2>
<div class="panel">
<h2>Discover our first monoclonal CRISPR/Cas9 antibody validated in ChIP<br /><br /></h2>
<div class="row">
<div class="small-5 medium-5 large-5 columns"><img src="/img/landing-pages/crispr-cas9-chip-on-hih3t3.jpg" alt="" /></div>
<div class="small-7 medium-7 large-7 columns">
<ul>
<li>Validated in chromatin immunoprecipitation</li>
<li>Performs better than FLAG antibody</li>
<li>Excellent for WB, IF and IP</li>
</ul>
<p><small><strong>ChIP</strong> was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 &micro;g chromatin was incubated overnight at 4&deg;C with 5 or 10 &micro;g of either an anti-FLAG antibody or the Diagenode antibody against Cas9 (Cat. No. C15200229). Mouse IgG was used as a negative IP control. qPCR was performed with primers specific for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns text-right"><a href="/p/crispr-cas9-monoclonal-antibody-50-ug-25-&mu;l" class="tiny details button radius">Learn more</a></div>
</div>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>First ChIP-grade CRISPR/Cas9 polyclonal antibody</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/landing-pages/c_a_s9-chip-grade-antibody.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Excellent polyclonal antibody for chromatin immunoprecipitation</li>
<li>Optimized for highest ChIP specificity and yields</li>
<li>Validated for all applications including immunoblotting, immunofluorescence and western blot</li>
</ul>
<p><small><strong>ChIP</strong> was performed on NIH3T3 cells stably expressing GFP- H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 &mu;g chromatin was incubated with either 5 &mu;g of an anti-FLAG antibody or 2 &mu;l of the Diagenode antibody against Cas9. The pre-immune serum (PPI) was used as negative IP control. Then qPCR was performed with primers specific for the GFP gene, and for two non-targeted regions: Ppap2c and Prkcd, used as negative controls. This figure shows the recovery, expressed as a % of input.</small></p>
<p class="text-right"><a href="../p/crispr-cas9-polyclonal-antibody" class="details tiny button">Learn more</a></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>CRISPR/Cas9 monoclonal antibody 4G10</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/landing-pages/cas9_4g10_fig1.png" width="170" height="302" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Antibody raised against N-terminus of Cas9 nuclease</li>
<li>Validated for western blot, IP and immunofluorescence</li>
</ul>
<p><small><strong>Immunofluorescence</strong>: Hela cells were transiently transfected with a Cas9 expression vector. The cells were fixed in 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA. The cells were stained with the Cas9 antibody at 4&deg;C o/n, followed by incubation with an anti mouse secondary antibody coupled to AF488 for 1 h at RT. Nuclei were counter-stained with Hoechst 33342 (right).</small></p>
<p class="text-right"><a href="../p/crispr-cas9-monoclonal-antibody-4g10-50-ug" class="details tiny button">Learn more</a></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>CRISPR/Cas9 C-terminal monoclonal antibody</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IP.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Antibody raised against C-terminus of Cas9 nuclease</li>
<li>Validated for western blot, IP and immunofluorescence</li>
</ul>
<p><small><strong> Western blot</strong> was performed on 20 &mu;g protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9. The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
<p class="text-right"><a href="../p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug" class="details tiny button">Learn more</a></p>
</div>
<div class="small-12 medium-12 large-12 columns">
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<a name="table"></a>
<table>
<thead>
<tr>
<th>Antibody</th>
<th>WB</th>
<th>IF</th>
<th>IP</th>
<th>ChIP</th>
<th>Antibody raised against</th>
</tr>
</thead>
<tbody>
<tr>
<td><a href="../p/crispr-cas9-monoclonal-antibody-50-ug-25-&mu;l"><strong class="diacol">CRISPR/Cas9 monoclonal antibody</strong></a></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><span class="diacol">N-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-polyclonal-antibody">CRISPR/Cas9 polyclonal antibody</a></td>
<td>++</td>
<td>++</td>
<td>++</td>
<td><strong class="diacol">+++</strong></td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-monoclonal-antibody-4g10-50-ug">CRISPR/Cas9 monoclonal antibody 4G10</a></td>
<td>+++</td>
<td>+++</td>
<td>++</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="../p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul"><strong class="diacol">CRISPR/Cas9 C-terminal monoclonal antibody</strong></a> <span class="label alert" style="font-size: 0.9rem;">NEW!</span></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">no</strong></td>
<td><strong class="diacol">+</strong></td>
<td><span class="diacol">C-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug">CRISPR/Cas9 C-terminal monoclonal antibody</a></td>
<td>++</td>
<td>++</td>
<td>+</td>
<td>no</td>
<td><span class="diacol">C-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-monoclonal-antibody-7A9-50-mg">CRISPR/Cas9 monoclonal antibody 7A9</a></td>
<td>++</td>
<td>++</td>
<td>++</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-hrp-monoclonal-antibody-50-ul">CRISPR/Cas9 - HRP monoclonal antibody 7A9</a></td>
<td>+++</td>
<td>no</td>
<td>no</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
</tbody>
</table>
</div>
</div>
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		'description' => '<p>Alternative name: <strong>Csn1</strong></p>
<p>Monoclonal antibody raised in mouse against the C-terminus of Cas9 nuclease (CRISPR-associated protein 9)</p>',
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15200223). The antibody was used at different dilutions. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-WB2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on 20 &micro;g protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9 (cat. No. C15200223). The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<div class="row">
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IP.png" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small><strong> Figure 3. IP using the Diagenode monoclonal antibody directed against Cas9 </strong><br />IP was performed on whole cell extracts (250 &amp; micro;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 13 &micro;g of the Diagenode antibody against Cas9 (cat. No. C15200223). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (15 &micro;g) is shown in lane 1 and 2. </small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (cat. No. C15200223) diluted 1:200, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488. Nuclei were counter-stained with Hoechst 33342. Figure 4 shows the result in the presence (left) or absence (right) of doxycycline. </small></p>
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                         <a href="/en/p/crispr-cas9-polyclonal-antibody"><img src="/img/product/antibodies/cas9-icon.png" alt="CRISPR/Cas9 Antibody " class="th"/></a>        </div>



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            <h6 style="height:60px">CRISPR/Cas9 Antibody</h6>
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	'description' => '<p>Polyclonal antibody raised in rabbit against the <strong>Cas9</strong> nuclease (<strong>CRISPR</strong>-associated protein 9) using a recombinant protein.&nbsp;</p>',
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-fig1.png" /></center></div>
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<p><strong>Figure 1. ChIP using the Diagenode antibody directed against Cas9</strong><br />ChIP was performed on sheared chromatin from 4 million HEK293T cells stably expressing nuclease dead Cas9 and sgRNA targeting a sequence in intron 8 of the GAPDH gene, using the iDeal ChIP-seq kit for transcription factors. A titration consisting of 1, 2, 5 and 10 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258) was tested. IgG (2 &micro;g/IP) was used as negative IP control. qPCR was performed with primers specific for the targeted sequence in the GAPDH gene, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns"><center>A.<img src="https://www.diagenode.com/img/product/antibodies/C15310258-chipseq-a.jpg" width="700" /></center><center>B.<img src="https://www.diagenode.com/img/product/antibodies/C15310258-chipseq-b.jpg" width="700" /></center></div>
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<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against Cas9</strong><br />ChIP was performed on sheared chromatin from 4 million HEK293T cells stably expressing dCas9 and a GAPDH sgRNA cells using 2 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the ChIP-seq profile in a region of chromosome 12 surrounding the GAPDH gene (fig 2B) and in a region of chromosome 2 surrounding an off-target peak in the YIPF4 gene.</p>
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<p><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with dCas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310258). The antibody was diluted 1:5,000. The marker is shown on the left, the position of the Cas9 protein is indicated on the right.</p>
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<div class="small-8 columns">
<p><strong>Figure 4. IP using the Diagenode antibody directed against Cas9</strong><br />IP was performed on whole cell extracts (500 &micro;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (25 &micro;g) is shown in lane 1 and 2.</p>
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<div class="small-12 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-IF.png" width="500" /></center></div>
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<p><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against Cas9</strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 antibody (cat. No. C15310258) diluted 1:1000, followed by incubation with a goat anti-rabbit secondary antibody coupled to AF594. Nuclei were counter-stained with Hoechst 33342. Figure 5 shows the result in the presence (left) or absence (right) of doxycycline.</p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong> Figure 3. IP using the Diagenode monoclonal antibody directed against Cas9 </strong><br />IP was performed on whole cell extracts (250 &mu;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 13 &mu;g of the Diagenode antibody against Cas9 (cat. No. C15200223). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (15 &mu;g) is shown in lane 1 and 2. </small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (cat. No. C15200223) diluted 1:200, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488. Nuclei were counter-stained with Hoechst 33342. Figure 4 shows the result in the presence (left) or absence (right) of doxycycline. </small></p>
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15200223). The antibody was used at different dilutions. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<p><small><strong> Figure 3. IP using the Diagenode monoclonal antibody directed against Cas9 </strong><br />IP was performed on whole cell extracts (250 &mu;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 13 &mu;g of the Diagenode antibody against Cas9 (cat. No. C15200223). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (15 &mu;g) is shown in lane 1 and 2. </small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (cat. No. C15200223) diluted 1:200, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488. Nuclei were counter-stained with Hoechst 33342. Figure 4 shows the result in the presence (left) or absence (right) of doxycycline. </small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on 20 &micro;g protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9 (cat. No. C15200223). The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<p><small><strong> Figure 3. IP using the Diagenode monoclonal antibody directed against Cas9 </strong><br />IP was performed on whole cell extracts (250 &amp; micro;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 13 &micro;g of the Diagenode antibody against Cas9 (cat. No. C15200223). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (15 &micro;g) is shown in lane 1 and 2. </small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (cat. No. C15200223) diluted 1:200, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488. Nuclei were counter-stained with Hoechst 33342. Figure 4 shows the result in the presence (left) or absence (right) of doxycycline. </small></p>
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<p><strong>Figure 1. ChIP using the Diagenode antibody directed against Cas9</strong><br />ChIP was performed on sheared chromatin from 4 million HEK293T cells stably expressing nuclease dead Cas9 and sgRNA targeting a sequence in intron 8 of the GAPDH gene, using the iDeal ChIP-seq kit for transcription factors. A titration consisting of 1, 2, 5 and 10 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258) was tested. IgG (2 &micro;g/IP) was used as negative IP control. qPCR was performed with primers specific for the targeted sequence in the GAPDH gene, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns"><center>A.<img src="https://www.diagenode.com/img/product/antibodies/C15310258-chipseq-a.jpg" width="700" /></center><center>B.<img src="https://www.diagenode.com/img/product/antibodies/C15310258-chipseq-b.jpg" width="700" /></center></div>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against Cas9</strong><br />ChIP was performed on sheared chromatin from 4 million HEK293T cells stably expressing dCas9 and a GAPDH sgRNA cells using 2 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the ChIP-seq profile in a region of chromosome 12 surrounding the GAPDH gene (fig 2B) and in a region of chromosome 2 surrounding an off-target peak in the YIPF4 gene.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-WB.png" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with dCas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310258). The antibody was diluted 1:5,000. The marker is shown on the left, the position of the Cas9 protein is indicated on the right.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-IP.png" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. IP using the Diagenode antibody directed against Cas9</strong><br />IP was performed on whole cell extracts (500 &micro;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (25 &micro;g) is shown in lane 1 and 2.</p>
</div>
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<div class="small-12 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15310258-CRISPR-IF.png" width="500" /></center></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against Cas9</strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 antibody (cat. No. C15310258) diluted 1:1000, followed by incubation with a goat anti-rabbit secondary antibody coupled to AF594. Nuclei were counter-stained with Hoechst 33342. Figure 5 shows the result in the presence (left) or absence (right) of doxycycline.</p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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			'description' => '<p>Diagenode offers the broad range of antibodies raised against the N- or C-terminus of the Cas9 nuclease from <em>Streptococcus <g class="gr_ gr_5 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="5" data-gr-id="5">pyogenes</g></em>. These highly specific polyclonal and monoclonal antibodies are validated in Western blot, immunoprecipitation, immunofluorescence and in chromatin immunoprecipitation.</p>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h2><em><a name="pyogenes"></a>S. pyogenes</em> CRISPR/Cas9 antibodies<a></a></h2>
<div class="panel">
<h2>Discover our first monoclonal CRISPR/Cas9 antibody validated in ChIP<br /><br /></h2>
<div class="row">
<div class="small-5 medium-5 large-5 columns"><img src="/img/landing-pages/crispr-cas9-chip-on-hih3t3.jpg" alt="" /></div>
<div class="small-7 medium-7 large-7 columns">
<ul>
<li>Validated in chromatin immunoprecipitation</li>
<li>Performs better than FLAG antibody</li>
<li>Excellent for WB, IF and IP</li>
</ul>
<p><small><strong>ChIP</strong> was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 &micro;g chromatin was incubated overnight at 4&deg;C with 5 or 10 &micro;g of either an anti-FLAG antibody or the Diagenode antibody against Cas9 (Cat. No. C15200229). Mouse IgG was used as a negative IP control. qPCR was performed with primers specific for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns text-right"><a href="/p/crispr-cas9-monoclonal-antibody-50-ug-25-&mu;l" class="tiny details button radius">Learn more</a></div>
</div>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>First ChIP-grade CRISPR/Cas9 polyclonal antibody</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/landing-pages/c_a_s9-chip-grade-antibody.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Excellent polyclonal antibody for chromatin immunoprecipitation</li>
<li>Optimized for highest ChIP specificity and yields</li>
<li>Validated for all applications including immunoblotting, immunofluorescence and western blot</li>
</ul>
<p><small><strong>ChIP</strong> was performed on NIH3T3 cells stably expressing GFP- H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 &mu;g chromatin was incubated with either 5 &mu;g of an anti-FLAG antibody or 2 &mu;l of the Diagenode antibody against Cas9. The pre-immune serum (PPI) was used as negative IP control. Then qPCR was performed with primers specific for the GFP gene, and for two non-targeted regions: Ppap2c and Prkcd, used as negative controls. This figure shows the recovery, expressed as a % of input.</small></p>
<p class="text-right"><a href="../p/crispr-cas9-polyclonal-antibody" class="details tiny button">Learn more</a></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>CRISPR/Cas9 monoclonal antibody 4G10</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/landing-pages/cas9_4g10_fig1.png" width="170" height="302" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Antibody raised against N-terminus of Cas9 nuclease</li>
<li>Validated for western blot, IP and immunofluorescence</li>
</ul>
<p><small><strong>Immunofluorescence</strong>: Hela cells were transiently transfected with a Cas9 expression vector. The cells were fixed in 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA. The cells were stained with the Cas9 antibody at 4&deg;C o/n, followed by incubation with an anti mouse secondary antibody coupled to AF488 for 1 h at RT. Nuclei were counter-stained with Hoechst 33342 (right).</small></p>
<p class="text-right"><a href="../p/crispr-cas9-monoclonal-antibody-4g10-50-ug" class="details tiny button">Learn more</a></p>
</div>
</div>
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<div class="small-12 medium-12 large-12 columns">
<h3>CRISPR/Cas9 C-terminal monoclonal antibody</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IP.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Antibody raised against C-terminus of Cas9 nuclease</li>
<li>Validated for western blot, IP and immunofluorescence</li>
</ul>
<p><small><strong> Western blot</strong> was performed on 20 &mu;g protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9. The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
<p class="text-right"><a href="../p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug" class="details tiny button">Learn more</a></p>
</div>
<div class="small-12 medium-12 large-12 columns">
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<a name="table"></a>
<table>
<thead>
<tr>
<th>Antibody</th>
<th>WB</th>
<th>IF</th>
<th>IP</th>
<th>ChIP</th>
<th>Antibody raised against</th>
</tr>
</thead>
<tbody>
<tr>
<td><a href="../p/crispr-cas9-monoclonal-antibody-50-ug-25-&mu;l"><strong class="diacol">CRISPR/Cas9 monoclonal antibody</strong></a></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><span class="diacol">N-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-polyclonal-antibody">CRISPR/Cas9 polyclonal antibody</a></td>
<td>++</td>
<td>++</td>
<td>++</td>
<td><strong class="diacol">+++</strong></td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-monoclonal-antibody-4g10-50-ug">CRISPR/Cas9 monoclonal antibody 4G10</a></td>
<td>+++</td>
<td>+++</td>
<td>++</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="../p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul"><strong class="diacol">CRISPR/Cas9 C-terminal monoclonal antibody</strong></a> <span class="label alert" style="font-size: 0.9rem;">NEW!</span></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">no</strong></td>
<td><strong class="diacol">+</strong></td>
<td><span class="diacol">C-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug">CRISPR/Cas9 C-terminal monoclonal antibody</a></td>
<td>++</td>
<td>++</td>
<td>+</td>
<td>no</td>
<td><span class="diacol">C-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-monoclonal-antibody-7A9-50-mg">CRISPR/Cas9 monoclonal antibody 7A9</a></td>
<td>++</td>
<td>++</td>
<td>++</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-hrp-monoclonal-antibody-50-ul">CRISPR/Cas9 - HRP monoclonal antibody 7A9</a></td>
<td>+++</td>
<td>no</td>
<td>no</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
</tbody>
</table>
</div>
</div>
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15200223). The antibody was used at different dilutions. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />Western blot was performed on 20 &micro;g protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9 (cat. No. C15200223). The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
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<p><small><strong> Figure 3. IP using the Diagenode monoclonal antibody directed against Cas9 </strong><br />IP was performed on whole cell extracts (250 &amp; micro;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 13 &micro;g of the Diagenode antibody against Cas9 (cat. No. C15200223). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (15 &micro;g) is shown in lane 1 and 2. </small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 </strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (cat. No. C15200223) diluted 1:200, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488. Nuclei were counter-stained with Hoechst 33342. Figure 4 shows the result in the presence (left) or absence (right) of doxycycline. </small></p>
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            <h6 style="height:60px">CRISPR/Cas9 Antibody</h6>
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<p><strong>Figure 1. ChIP using the Diagenode antibody directed against Cas9</strong><br />ChIP was performed on sheared chromatin from 4 million HEK293T cells stably expressing nuclease dead Cas9 and sgRNA targeting a sequence in intron 8 of the GAPDH gene, using the iDeal ChIP-seq kit for transcription factors. A titration consisting of 1, 2, 5 and 10 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258) was tested. IgG (2 &micro;g/IP) was used as negative IP control. qPCR was performed with primers specific for the targeted sequence in the GAPDH gene, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns"><center>A.<img src="https://www.diagenode.com/img/product/antibodies/C15310258-chipseq-a.jpg" width="700" /></center><center>B.<img src="https://www.diagenode.com/img/product/antibodies/C15310258-chipseq-b.jpg" width="700" /></center></div>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against Cas9</strong><br />ChIP was performed on sheared chromatin from 4 million HEK293T cells stably expressing dCas9 and a GAPDH sgRNA cells using 2 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the ChIP-seq profile in a region of chromosome 12 surrounding the GAPDH gene (fig 2B) and in a region of chromosome 2 surrounding an off-target peak in the YIPF4 gene.</p>
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<p><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with dCas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310258). The antibody was diluted 1:5,000. The marker is shown on the left, the position of the Cas9 protein is indicated on the right.</p>
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<p><strong>Figure 4. IP using the Diagenode antibody directed against Cas9</strong><br />IP was performed on whole cell extracts (500 &micro;g) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 &micro;l of the Diagenode antibody against Cas9 (cat. No. C15310258). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (25 &micro;g) is shown in lane 1 and 2.</p>
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<p><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against Cas9</strong><br />HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 antibody (cat. No. C15310258) diluted 1:1000, followed by incubation with a goat anti-rabbit secondary antibody coupled to AF594. Nuclei were counter-stained with Hoechst 33342. Figure 5 shows the result in the presence (left) or absence (right) of doxycycline.</p>
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	'meta_keywords' => 'CRISPR/Cas9 C-terminal monoclonal antibody,Western blot,diagenode',
	'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated  in WB, IP and IF. Batch-specific data available on the website. Alternative name: Csn1. Sample size available.',
	'modified' => '2022-01-05 15:11:19',
	'created' => '2015-08-26 16:29:59',
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)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '30',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'IP',
	'description' => '<p>Immunoprecipitation</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'immunoprecipitation',
	'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
	'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
	'modified' => '2016-01-13 12:23:07',
	'created' => '2015-07-08 13:46:50',
	'ProductsApplication' => array(
		'id' => '3999',
		'product_id' => '2764',
		'application_id' => '30'
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)
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	(int) 0 => 'immunoprecipitation'
)
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	'name' => 'IP',
	'description' => '<p>Immunoprecipitation</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'immunoprecipitation',
	'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
	'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
	'modified' => '2016-01-13 12:23:07',
	'created' => '2015-07-08 13:46:50',
	'locale' => 'eng'
)
$description = '<p>Immunoprecipitation</p>'
$name = 'IP'
$document = array(
	'id' => '799',
	'name' => 'Datasheet C15200223 CRISPR Cas9 Csn1 monoclonal antibody',
	'description' => 'Datasheet description',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/antibodies/Datasheet_C15200223_CRISPR_Cas9_Csn1_monoclonal_antibody.pdf',
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		'product_id' => '2764',
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$sds = array(
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	'language' => 'nl',
	'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200223-CRISPR_Cas9_C-terminal_Antibody-BE-nl-GHS_2_0.pdf',
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	'created' => '2021-01-26 12:40:32',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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