Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).
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Alternative names:ESR, ESR1, ESRA, NR3A1
Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).
| Lot | 001 |
|---|---|
| Concentration | 2.0 µg/µl |
| Species reactivity | Human |
| Type | Monoclonal |
| Purity | Ammonium sulphate purified |
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP * | 5 μg/ChIP | Fig 1 |
| Western Blotting | 7 μg/ml | Fig 2 |
| Immunochemistry | 15 μg/ml | Fig 3 |
Figure 1. ChIP using the Diagenode monoclonal antibody
ChIP using the Diagenode monoclonal antibody against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.
Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα
Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.
Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα
COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.
Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
| IC Immunochemistry Read more |
| Datasheet hERalpha MAb-009-050 DATASHEET Datasheet description | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: ER alpha Antibody (Diagenode Cat# C15200009 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer |
An oestrogen-receptor-alpha-bound human chromatin interactome. |
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ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. 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ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. 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ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '505', 'name' => 'Datasheet hERalpha MAb-009-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_hERalpha_MAb-009-050.pdf', 'slug' => 'datasheet-heralpha-mab-009-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1779', 'name' => 'product/antibodies/ab-chip-icon.png', 'alt' => 'Antibody ChIP icon', 'modified' => '2020-08-12 11:52:55', 'created' => '2018-03-15 15:52:35', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3363', 'name' => 'Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer', 'authors' => 'Adurthi S. et al.', 'description' => '<p>Oestrogen controls Foxp3 expression in regulatory T cells (T<sub>reg</sub> cells) via a mechanism thought to involve oestrogen receptor alpha (ERα), but the molecular basis and functional impact of ERα signalling in T<sub>reg</sub> cells remain unclear. We report that ERα ligand oestradiol (E2) is significantly increased in human cervical cancer (CxCa) tissues and tumour-infiltrating T<sub>reg</sub> cells (CD4<sup>+</sup>CD25<sup>hi</sup>CD127<sup>low</sup>), whereas blocking ERα with the antagonist ICI 182,780 abolishes FOXP3 expression and impairs the function of CxCa infiltrating T<sub>reg</sub> cells. Using a novel approach of co-immunoprecipitation with antibodies to E2 for capture, we identified binding of E2:ERα complexes to FOXP3 protein in CxCa-derived T<sub>reg</sub> cells. Chromatin immunoprecipitation analyses of male blood T<sub>reg</sub> cells revealed ERα occupancy at the FOXP3 promoter and conserved non-coding DNA elements 2 and 3. Accordingly, computational analyses of the enriched regions uncovered eight putative oestrogen response elements predicted to form a loop that can activate the FOXP3 promoter. Together, these data suggest that E2-mediated ERα signalling is critical for the sustenance of FOXP3 expression and T<sub>reg</sub> cell function in human CxCa via direct interaction of ERα with FOXP3 promoter. Overall, our work gives a molecular insight into ERα signalling and highlights a fundamental role of E2 in controlling human T<sub>reg</sub> cell physiology.</p>', 'date' => '2017-12-11', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/29229929', 'doi' => '', 'modified' => '2018-04-24 10:07:53', 'created' => '2018-04-24 10:07:53', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '66', 'name' => 'An oestrogen-receptor-alpha-bound human chromatin interactome.', 'authors' => 'Fullwood MJ, Liu MH, Pan YF, Liu J, Xu H, Mohamed YB, Orlov YL, Velkov S, Ho A, Mei PH, Chew EG, Huang PY, Welboren WJ, Han Y, Ooi HS, Ariyaratne PN, Vega VB, Luo Y, Tan PY, Choy PY, Wansa KD, Zhao B, Lim KS, Leow SC, Yow JS, Joseph R, Li H, Desai KV, Tho', 'description' => 'Genomes are organized into high-level three-dimensional structures, and DNA elements separated by long genomic distances can in principle interact functionally. Many transcription factors bind to regulatory DNA elements distant from gene promoters. Although distal binding sites have been shown to regulate transcription by long-range chromatin interactions at a few loci, chromatin interactions and their impact on transcription regulation have not been investigated in a genome-wide manner. Here we describe the development of a new strategy, chromatin interaction analysis by paired-end tag sequencing (ChIA-PET) for the de novo detection of global chromatin interactions, with which we have comprehensively mapped the chromatin interaction network bound by oestrogen receptor alpha (ER-alpha) in the human genome. We found that most high-confidence remote ER-alpha-binding sites are anchored at gene promoters through long-range chromatin interactions, suggesting that ER-alpha functions by extensive chromatin looping to bring genes together for coordinated transcriptional regulation. We propose that chromatin interactions constitute a primary mechanism for regulating transcription in mammalian genomes.', 'date' => '2009-11-05', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/19890323', 'doi' => '', 'modified' => '2015-07-24 15:38:56', 'created' => '2015-07-24 15:38:56', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '574', 'name' => 'ER alpha antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2020-07-01 14:58:20', 'created' => '2020-07-01 14:58:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '576', 'name' => 'ER alpha antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2020-07-01 14:59:24', 'created' => '2020-07-01 14:59:24', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '571', 'name' => 'ER alpha antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2020-07-01 14:56:44', 'created' => '2020-07-01 14:56:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '575', 'name' => 'ER alpha antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-JP-ja-GHS_2_0.pdf', 'countries' => 'JP', 'modified' => '2020-07-01 14:58:55', 'created' => '2020-07-01 14:58:55', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '570', 'name' => 'ER alpha antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:56:20', 'created' => '2020-07-01 14:56:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '569', 'name' => 'ER alpha antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:55:44', 'created' => '2020-07-01 14:55:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '573', 'name' => 'ER alpha antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2020-07-01 14:57:51', 'created' => '2020-07-01 14:57:51', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '572', 'name' => 'ER alpha antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2020-07-01 14:57:14', 'created' => '2020-07-01 14:57:14', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/er-alpha-monoclonal-antibody-classic-50-ug-25-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '27', 'product_id' => '1969', 'group_id' => '20' ) ) ) $pro = array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '27', 'product_id' => '1969', 'group_id' => '20' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(MAb-009-050)</span>' $country_code = 'US' $other_format = array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. 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ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( 'id' => '2023', 'product_id' => '1968', 'document_id' => '11' ) ) $sds = array( 'id' => '572', 'name' => 'ER alpha antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2020-07-01 14:57:14', 'created' => '2020-07-01 14:57:14', 'ProductsSafetySheet' => array( 'id' => '1102', 'product_id' => '1968', 'safety_sheet_id' => '572' ) ) $publication = array( 'id' => '66', 'name' => 'An oestrogen-receptor-alpha-bound human chromatin interactome.', 'authors' => 'Fullwood MJ, Liu MH, Pan YF, Liu J, Xu H, Mohamed YB, Orlov YL, Velkov S, Ho A, Mei PH, Chew EG, Huang PY, Welboren WJ, Han Y, Ooi HS, Ariyaratne PN, Vega VB, Luo Y, Tan PY, Choy PY, Wansa KD, Zhao B, Lim KS, Leow SC, Yow JS, Joseph R, Li H, Desai KV, Tho', 'description' => 'Genomes are organized into high-level three-dimensional structures, and DNA elements separated by long genomic distances can in principle interact functionally. Many transcription factors bind to regulatory DNA elements distant from gene promoters. Although distal binding sites have been shown to regulate transcription by long-range chromatin interactions at a few loci, chromatin interactions and their impact on transcription regulation have not been investigated in a genome-wide manner. Here we describe the development of a new strategy, chromatin interaction analysis by paired-end tag sequencing (ChIA-PET) for the de novo detection of global chromatin interactions, with which we have comprehensively mapped the chromatin interaction network bound by oestrogen receptor alpha (ER-alpha) in the human genome. We found that most high-confidence remote ER-alpha-binding sites are anchored at gene promoters through long-range chromatin interactions, suggesting that ER-alpha functions by extensive chromatin looping to bring genes together for coordinated transcriptional regulation. We propose that chromatin interactions constitute a primary mechanism for regulating transcription in mammalian genomes.', 'date' => '2009-11-05', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/19890323', 'doi' => '', 'modified' => '2015-07-24 15:38:56', 'created' => '2015-07-24 15:38:56', 'ProductsPublication' => array( 'id' => '771', 'product_id' => '1968', 'publication_id' => '66' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/19890323" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'product' => array( 'Product' => array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. 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ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). 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Sample size available.', 'modified' => '2021-12-23 12:07:23', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '103', 'name' => 'ER alpha monoclonal antibody', 'description' => 'Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '2.0 µg/µl', 'reactivity' => 'Human', 'type' => 'Monoclonal', 'purity' => 'Ammonium sulphate purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP <sup>*</sup> </td> <td>5 μg/ChIP</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>7 μg/ml</td> <td>Fig 2</td> </tr> <tr> <td>Immunochemistry</td> <td>15 μg/ml</td> <td>Fig 3</td> </tr> </tbody> </table> <small><sup>*</sup> Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-14 10:03:25', 'created' => '0000-00-00 00:00:00', 'select_label' => '103 - ER alpha monoclonal antibody (001 - 2.0 µg/µl - Human - Ammonium sulphate purified - Mouse)' ), 'Slave' => array( (int) 0 => array( 'id' => '20', 'name' => 'C15200009', 'product_id' => '1968', 'modified' => '2016-02-18 18:02:12', 'created' => '2016-02-18 18:02:12' ) ), 'Group' => array( 'Group' => array( 'id' => '20', 'name' => 'C15200009', 'product_id' => '1968', 'modified' => '2016-02-18 18:02:12', 'created' => '2016-02-18 18:02:12' ), 'Master' => array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:23', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '505', 'name' => 'Datasheet hERalpha MAb-009-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_hERalpha_MAb-009-050.pdf', 'slug' => 'datasheet-heralpha-mab-009-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1779', 'name' => 'product/antibodies/ab-chip-icon.png', 'alt' => 'Antibody ChIP icon', 'modified' => '2020-08-12 11:52:55', 'created' => '2018-03-15 15:52:35', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3363', 'name' => 'Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer', 'authors' => 'Adurthi S. et al.', 'description' => '<p>Oestrogen controls Foxp3 expression in regulatory T cells (T<sub>reg</sub> cells) via a mechanism thought to involve oestrogen receptor alpha (ERα), but the molecular basis and functional impact of ERα signalling in T<sub>reg</sub> cells remain unclear. We report that ERα ligand oestradiol (E2) is significantly increased in human cervical cancer (CxCa) tissues and tumour-infiltrating T<sub>reg</sub> cells (CD4<sup>+</sup>CD25<sup>hi</sup>CD127<sup>low</sup>), whereas blocking ERα with the antagonist ICI 182,780 abolishes FOXP3 expression and impairs the function of CxCa infiltrating T<sub>reg</sub> cells. Using a novel approach of co-immunoprecipitation with antibodies to E2 for capture, we identified binding of E2:ERα complexes to FOXP3 protein in CxCa-derived T<sub>reg</sub> cells. Chromatin immunoprecipitation analyses of male blood T<sub>reg</sub> cells revealed ERα occupancy at the FOXP3 promoter and conserved non-coding DNA elements 2 and 3. Accordingly, computational analyses of the enriched regions uncovered eight putative oestrogen response elements predicted to form a loop that can activate the FOXP3 promoter. Together, these data suggest that E2-mediated ERα signalling is critical for the sustenance of FOXP3 expression and T<sub>reg</sub> cell function in human CxCa via direct interaction of ERα with FOXP3 promoter. Overall, our work gives a molecular insight into ERα signalling and highlights a fundamental role of E2 in controlling human T<sub>reg</sub> cell physiology.</p>', 'date' => '2017-12-11', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/29229929', 'doi' => '', 'modified' => '2018-04-24 10:07:53', 'created' => '2018-04-24 10:07:53', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '66', 'name' => 'An oestrogen-receptor-alpha-bound human chromatin interactome.', 'authors' => 'Fullwood MJ, Liu MH, Pan YF, Liu J, Xu H, Mohamed YB, Orlov YL, Velkov S, Ho A, Mei PH, Chew EG, Huang PY, Welboren WJ, Han Y, Ooi HS, Ariyaratne PN, Vega VB, Luo Y, Tan PY, Choy PY, Wansa KD, Zhao B, Lim KS, Leow SC, Yow JS, Joseph R, Li H, Desai KV, Tho', 'description' => 'Genomes are organized into high-level three-dimensional structures, and DNA elements separated by long genomic distances can in principle interact functionally. Many transcription factors bind to regulatory DNA elements distant from gene promoters. Although distal binding sites have been shown to regulate transcription by long-range chromatin interactions at a few loci, chromatin interactions and their impact on transcription regulation have not been investigated in a genome-wide manner. Here we describe the development of a new strategy, chromatin interaction analysis by paired-end tag sequencing (ChIA-PET) for the de novo detection of global chromatin interactions, with which we have comprehensively mapped the chromatin interaction network bound by oestrogen receptor alpha (ER-alpha) in the human genome. We found that most high-confidence remote ER-alpha-binding sites are anchored at gene promoters through long-range chromatin interactions, suggesting that ER-alpha functions by extensive chromatin looping to bring genes together for coordinated transcriptional regulation. We propose that chromatin interactions constitute a primary mechanism for regulating transcription in mammalian genomes.', 'date' => '2009-11-05', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/19890323', 'doi' => '', 'modified' => '2015-07-24 15:38:56', 'created' => '2015-07-24 15:38:56', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '574', 'name' => 'ER alpha antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2020-07-01 14:58:20', 'created' => '2020-07-01 14:58:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '576', 'name' => 'ER alpha antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2020-07-01 14:59:24', 'created' => '2020-07-01 14:59:24', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '571', 'name' => 'ER alpha antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2020-07-01 14:56:44', 'created' => '2020-07-01 14:56:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '575', 'name' => 'ER alpha antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-JP-ja-GHS_2_0.pdf', 'countries' => 'JP', 'modified' => '2020-07-01 14:58:55', 'created' => '2020-07-01 14:58:55', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '570', 'name' => 'ER alpha antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:56:20', 'created' => '2020-07-01 14:56:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '569', 'name' => 'ER alpha antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:55:44', 'created' => '2020-07-01 14:55:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '573', 'name' => 'ER alpha antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2020-07-01 14:57:51', 'created' => '2020-07-01 14:57:51', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '572', 'name' => 'ER alpha antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2020-07-01 14:57:14', 'created' => '2020-07-01 14:57:14', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/er-alpha-monoclonal-antibody-classic-50-ug-25-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '27', 'product_id' => '1969', 'group_id' => '20' ) ) ) $pro = array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '27', 'product_id' => '1969', 'group_id' => '20' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(MAb-009-050)</span>' $country_code = 'US' $other_format = array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '27', 'product_id' => '1969', 'group_id' => '20' ) ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'ProductsApplication' => array( 'id' => '5058', 'product_id' => '1968', 'application_id' => '31' ) ) $slugs = array( (int) 0 => 'immunochemistry' ) $applications = array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'locale' => 'eng' ) $description = '<p>Immunochemistry</p>' $name = 'IC' $document = array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( 'id' => '2023', 'product_id' => '1968', 'document_id' => '11' ) ) $sds = array( 'id' => '572', 'name' => 'ER alpha antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2020-07-01 14:57:14', 'created' => '2020-07-01 14:57:14', 'ProductsSafetySheet' => array( 'id' => '1102', 'product_id' => '1968', 'safety_sheet_id' => '572' ) ) $publication = array( 'id' => '66', 'name' => 'An oestrogen-receptor-alpha-bound human chromatin interactome.', 'authors' => 'Fullwood MJ, Liu MH, Pan YF, Liu J, Xu H, Mohamed YB, Orlov YL, Velkov S, Ho A, Mei PH, Chew EG, Huang PY, Welboren WJ, Han Y, Ooi HS, Ariyaratne PN, Vega VB, Luo Y, Tan PY, Choy PY, Wansa KD, Zhao B, Lim KS, Leow SC, Yow JS, Joseph R, Li H, Desai KV, Tho', 'description' => 'Genomes are organized into high-level three-dimensional structures, and DNA elements separated by long genomic distances can in principle interact functionally. Many transcription factors bind to regulatory DNA elements distant from gene promoters. Although distal binding sites have been shown to regulate transcription by long-range chromatin interactions at a few loci, chromatin interactions and their impact on transcription regulation have not been investigated in a genome-wide manner. Here we describe the development of a new strategy, chromatin interaction analysis by paired-end tag sequencing (ChIA-PET) for the de novo detection of global chromatin interactions, with which we have comprehensively mapped the chromatin interaction network bound by oestrogen receptor alpha (ER-alpha) in the human genome. We found that most high-confidence remote ER-alpha-binding sites are anchored at gene promoters through long-range chromatin interactions, suggesting that ER-alpha functions by extensive chromatin looping to bring genes together for coordinated transcriptional regulation. We propose that chromatin interactions constitute a primary mechanism for regulating transcription in mammalian genomes.', 'date' => '2009-11-05', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/19890323', 'doi' => '', 'modified' => '2015-07-24 15:38:56', 'created' => '2015-07-24 15:38:56', 'ProductsPublication' => array( 'id' => '771', 'product_id' => '1968', 'publication_id' => '66' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/19890323" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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Sample size available.', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'product' => array( 'Product' => array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. 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We recommend testing 1-5 μg per IP.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-14 10:03:25', 'created' => '0000-00-00 00:00:00', 'select_label' => '103 - ER alpha monoclonal antibody (001 - 2.0 µg/µl - Human - Ammonium sulphate purified - Mouse)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/er-alpha-monoclonal-antibody-classic-50-ug-25-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.' $meta_title = 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode' $product = array( 'Product' => array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:23', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '103', 'name' => 'ER alpha monoclonal antibody', 'description' => 'Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '2.0 µg/µl', 'reactivity' => 'Human', 'type' => 'Monoclonal', 'purity' => 'Ammonium sulphate purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP <sup>*</sup> </td> <td>5 μg/ChIP</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>7 μg/ml</td> <td>Fig 2</td> </tr> <tr> <td>Immunochemistry</td> <td>15 μg/ml</td> <td>Fig 3</td> </tr> </tbody> </table> <small><sup>*</sup> Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-14 10:03:25', 'created' => '0000-00-00 00:00:00', 'select_label' => '103 - ER alpha monoclonal antibody (001 - 2.0 µg/µl - Human - Ammonium sulphate purified - Mouse)' ), 'Slave' => array( (int) 0 => array( 'id' => '20', 'name' => 'C15200009', 'product_id' => '1968', 'modified' => '2016-02-18 18:02:12', 'created' => '2016-02-18 18:02:12' ) ), 'Group' => array( 'Group' => array( 'id' => '20', 'name' => 'C15200009', 'product_id' => '1968', 'modified' => '2016-02-18 18:02:12', 'created' => '2016-02-18 18:02:12' ), 'Master' => array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:23', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '505', 'name' => 'Datasheet hERalpha MAb-009-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_hERalpha_MAb-009-050.pdf', 'slug' => 'datasheet-heralpha-mab-009-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1779', 'name' => 'product/antibodies/ab-chip-icon.png', 'alt' => 'Antibody ChIP icon', 'modified' => '2020-08-12 11:52:55', 'created' => '2018-03-15 15:52:35', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3363', 'name' => 'Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer', 'authors' => 'Adurthi S. et al.', 'description' => '<p>Oestrogen controls Foxp3 expression in regulatory T cells (T<sub>reg</sub> cells) via a mechanism thought to involve oestrogen receptor alpha (ERα), but the molecular basis and functional impact of ERα signalling in T<sub>reg</sub> cells remain unclear. We report that ERα ligand oestradiol (E2) is significantly increased in human cervical cancer (CxCa) tissues and tumour-infiltrating T<sub>reg</sub> cells (CD4<sup>+</sup>CD25<sup>hi</sup>CD127<sup>low</sup>), whereas blocking ERα with the antagonist ICI 182,780 abolishes FOXP3 expression and impairs the function of CxCa infiltrating T<sub>reg</sub> cells. Using a novel approach of co-immunoprecipitation with antibodies to E2 for capture, we identified binding of E2:ERα complexes to FOXP3 protein in CxCa-derived T<sub>reg</sub> cells. Chromatin immunoprecipitation analyses of male blood T<sub>reg</sub> cells revealed ERα occupancy at the FOXP3 promoter and conserved non-coding DNA elements 2 and 3. Accordingly, computational analyses of the enriched regions uncovered eight putative oestrogen response elements predicted to form a loop that can activate the FOXP3 promoter. Together, these data suggest that E2-mediated ERα signalling is critical for the sustenance of FOXP3 expression and T<sub>reg</sub> cell function in human CxCa via direct interaction of ERα with FOXP3 promoter. Overall, our work gives a molecular insight into ERα signalling and highlights a fundamental role of E2 in controlling human T<sub>reg</sub> cell physiology.</p>', 'date' => '2017-12-11', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/29229929', 'doi' => '', 'modified' => '2018-04-24 10:07:53', 'created' => '2018-04-24 10:07:53', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '66', 'name' => 'An oestrogen-receptor-alpha-bound human chromatin interactome.', 'authors' => 'Fullwood MJ, Liu MH, Pan YF, Liu J, Xu H, Mohamed YB, Orlov YL, Velkov S, Ho A, Mei PH, Chew EG, Huang PY, Welboren WJ, Han Y, Ooi HS, Ariyaratne PN, Vega VB, Luo Y, Tan PY, Choy PY, Wansa KD, Zhao B, Lim KS, Leow SC, Yow JS, Joseph R, Li H, Desai KV, Tho', 'description' => 'Genomes are organized into high-level three-dimensional structures, and DNA elements separated by long genomic distances can in principle interact functionally. Many transcription factors bind to regulatory DNA elements distant from gene promoters. Although distal binding sites have been shown to regulate transcription by long-range chromatin interactions at a few loci, chromatin interactions and their impact on transcription regulation have not been investigated in a genome-wide manner. Here we describe the development of a new strategy, chromatin interaction analysis by paired-end tag sequencing (ChIA-PET) for the de novo detection of global chromatin interactions, with which we have comprehensively mapped the chromatin interaction network bound by oestrogen receptor alpha (ER-alpha) in the human genome. We found that most high-confidence remote ER-alpha-binding sites are anchored at gene promoters through long-range chromatin interactions, suggesting that ER-alpha functions by extensive chromatin looping to bring genes together for coordinated transcriptional regulation. 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ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. 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ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). 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Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. 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ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). 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Many transcription factors bind to regulatory DNA elements distant from gene promoters. Although distal binding sites have been shown to regulate transcription by long-range chromatin interactions at a few loci, chromatin interactions and their impact on transcription regulation have not been investigated in a genome-wide manner. Here we describe the development of a new strategy, chromatin interaction analysis by paired-end tag sequencing (ChIA-PET) for the de novo detection of global chromatin interactions, with which we have comprehensively mapped the chromatin interaction network bound by oestrogen receptor alpha (ER-alpha) in the human genome. We found that most high-confidence remote ER-alpha-binding sites are anchored at gene promoters through long-range chromatin interactions, suggesting that ER-alpha functions by extensive chromatin looping to bring genes together for coordinated transcriptional regulation. 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'product' => array( 'Product' => array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. 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Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.' $meta_title = 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode' $product = array( 'Product' => array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. 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We recommend testing 1-5 μg per IP.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-14 10:03:25', 'created' => '0000-00-00 00:00:00', 'select_label' => '103 - ER alpha monoclonal antibody (001 - 2.0 µg/µl - Human - Ammonium sulphate purified - Mouse)' ), 'Slave' => array( (int) 0 => array( 'id' => '20', 'name' => 'C15200009', 'product_id' => '1968', 'modified' => '2016-02-18 18:02:12', 'created' => '2016-02-18 18:02:12' ) ), 'Group' => array( 'Group' => array( 'id' => '20', 'name' => 'C15200009', 'product_id' => '1968', 'modified' => '2016-02-18 18:02:12', 'created' => '2016-02-18 18:02:12' ), 'Master' => array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:23', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '505', 'name' => 'Datasheet hERalpha MAb-009-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_hERalpha_MAb-009-050.pdf', 'slug' => 'datasheet-heralpha-mab-009-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1779', 'name' => 'product/antibodies/ab-chip-icon.png', 'alt' => 'Antibody ChIP icon', 'modified' => '2020-08-12 11:52:55', 'created' => '2018-03-15 15:52:35', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3363', 'name' => 'Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer', 'authors' => 'Adurthi S. et al.', 'description' => '<p>Oestrogen controls Foxp3 expression in regulatory T cells (T<sub>reg</sub> cells) via a mechanism thought to involve oestrogen receptor alpha (ERα), but the molecular basis and functional impact of ERα signalling in T<sub>reg</sub> cells remain unclear. We report that ERα ligand oestradiol (E2) is significantly increased in human cervical cancer (CxCa) tissues and tumour-infiltrating T<sub>reg</sub> cells (CD4<sup>+</sup>CD25<sup>hi</sup>CD127<sup>low</sup>), whereas blocking ERα with the antagonist ICI 182,780 abolishes FOXP3 expression and impairs the function of CxCa infiltrating T<sub>reg</sub> cells. Using a novel approach of co-immunoprecipitation with antibodies to E2 for capture, we identified binding of E2:ERα complexes to FOXP3 protein in CxCa-derived T<sub>reg</sub> cells. Chromatin immunoprecipitation analyses of male blood T<sub>reg</sub> cells revealed ERα occupancy at the FOXP3 promoter and conserved non-coding DNA elements 2 and 3. Accordingly, computational analyses of the enriched regions uncovered eight putative oestrogen response elements predicted to form a loop that can activate the FOXP3 promoter. Together, these data suggest that E2-mediated ERα signalling is critical for the sustenance of FOXP3 expression and T<sub>reg</sub> cell function in human CxCa via direct interaction of ERα with FOXP3 promoter. Overall, our work gives a molecular insight into ERα signalling and highlights a fundamental role of E2 in controlling human T<sub>reg</sub> cell physiology.</p>', 'date' => '2017-12-11', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/29229929', 'doi' => '', 'modified' => '2018-04-24 10:07:53', 'created' => '2018-04-24 10:07:53', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '66', 'name' => 'An oestrogen-receptor-alpha-bound human chromatin interactome.', 'authors' => 'Fullwood MJ, Liu MH, Pan YF, Liu J, Xu H, Mohamed YB, Orlov YL, Velkov S, Ho A, Mei PH, Chew EG, Huang PY, Welboren WJ, Han Y, Ooi HS, Ariyaratne PN, Vega VB, Luo Y, Tan PY, Choy PY, Wansa KD, Zhao B, Lim KS, Leow SC, Yow JS, Joseph R, Li H, Desai KV, Tho', 'description' => 'Genomes are organized into high-level three-dimensional structures, and DNA elements separated by long genomic distances can in principle interact functionally. Many transcription factors bind to regulatory DNA elements distant from gene promoters. Although distal binding sites have been shown to regulate transcription by long-range chromatin interactions at a few loci, chromatin interactions and their impact on transcription regulation have not been investigated in a genome-wide manner. Here we describe the development of a new strategy, chromatin interaction analysis by paired-end tag sequencing (ChIA-PET) for the de novo detection of global chromatin interactions, with which we have comprehensively mapped the chromatin interaction network bound by oestrogen receptor alpha (ER-alpha) in the human genome. We found that most high-confidence remote ER-alpha-binding sites are anchored at gene promoters through long-range chromatin interactions, suggesting that ER-alpha functions by extensive chromatin looping to bring genes together for coordinated transcriptional regulation. We propose that chromatin interactions constitute a primary mechanism for regulating transcription in mammalian genomes.', 'date' => '2009-11-05', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/19890323', 'doi' => '', 'modified' => '2015-07-24 15:38:56', 'created' => '2015-07-24 15:38:56', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '574', 'name' => 'ER alpha antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2020-07-01 14:58:20', 'created' => '2020-07-01 14:58:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '576', 'name' => 'ER alpha antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2020-07-01 14:59:24', 'created' => '2020-07-01 14:59:24', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '571', 'name' => 'ER alpha antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2020-07-01 14:56:44', 'created' => '2020-07-01 14:56:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '575', 'name' => 'ER alpha antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-JP-ja-GHS_2_0.pdf', 'countries' => 'JP', 'modified' => '2020-07-01 14:58:55', 'created' => '2020-07-01 14:58:55', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '570', 'name' => 'ER alpha antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:56:20', 'created' => '2020-07-01 14:56:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '569', 'name' => 'ER alpha antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:55:44', 'created' => '2020-07-01 14:55:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '573', 'name' => 'ER alpha antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2020-07-01 14:57:51', 'created' => '2020-07-01 14:57:51', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '572', 'name' => 'ER alpha antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2020-07-01 14:57:14', 'created' => '2020-07-01 14:57:14', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/er-alpha-monoclonal-antibody-classic-50-ug-25-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '27', 'product_id' => '1969', 'group_id' => '20' ) ) ) $pro = array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '27', 'product_id' => '1969', 'group_id' => '20' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(MAb-009-050)</span>' $country_code = 'US' $other_format = array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '27', 'product_id' => '1969', 'group_id' => '20' ) ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'ProductsApplication' => array( 'id' => '5058', 'product_id' => '1968', 'application_id' => '31' ) ) $slugs = array( (int) 0 => 'immunochemistry' ) $applications = array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'locale' => 'eng' ) $description = '<p>Immunochemistry</p>' $name = 'IC' $document = array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( 'id' => '2023', 'product_id' => '1968', 'document_id' => '11' ) ) $sds = array( 'id' => '572', 'name' => 'ER alpha antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2020-07-01 14:57:14', 'created' => '2020-07-01 14:57:14', 'ProductsSafetySheet' => array( 'id' => '1102', 'product_id' => '1968', 'safety_sheet_id' => '572' ) ) $publication = array( 'id' => '66', 'name' => 'An oestrogen-receptor-alpha-bound human chromatin interactome.', 'authors' => 'Fullwood MJ, Liu MH, Pan YF, Liu J, Xu H, Mohamed YB, Orlov YL, Velkov S, Ho A, Mei PH, Chew EG, Huang PY, Welboren WJ, Han Y, Ooi HS, Ariyaratne PN, Vega VB, Luo Y, Tan PY, Choy PY, Wansa KD, Zhao B, Lim KS, Leow SC, Yow JS, Joseph R, Li H, Desai KV, Tho', 'description' => 'Genomes are organized into high-level three-dimensional structures, and DNA elements separated by long genomic distances can in principle interact functionally. Many transcription factors bind to regulatory DNA elements distant from gene promoters. Although distal binding sites have been shown to regulate transcription by long-range chromatin interactions at a few loci, chromatin interactions and their impact on transcription regulation have not been investigated in a genome-wide manner. Here we describe the development of a new strategy, chromatin interaction analysis by paired-end tag sequencing (ChIA-PET) for the de novo detection of global chromatin interactions, with which we have comprehensively mapped the chromatin interaction network bound by oestrogen receptor alpha (ER-alpha) in the human genome. We found that most high-confidence remote ER-alpha-binding sites are anchored at gene promoters through long-range chromatin interactions, suggesting that ER-alpha functions by extensive chromatin looping to bring genes together for coordinated transcriptional regulation. We propose that chromatin interactions constitute a primary mechanism for regulating transcription in mammalian genomes.', 'date' => '2009-11-05', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/19890323', 'doi' => '', 'modified' => '2015-07-24 15:38:56', 'created' => '2015-07-24 15:38:56', 'ProductsPublication' => array( 'id' => '771', 'product_id' => '1968', 'publication_id' => '66' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/19890323" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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