Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).
Request a quote for a bulk order for ER alpha Antibody (sample size). Please fill out the form here below. Your local sales account manager will get in touch with you shortly and send you a quotation based on your requirements.
Alternative names: ESR, ESR1, ESRA, NR3A1
Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).
| Lot | 001 |
|---|---|
| Concentration | 2.0 µg/µl |
| Species reactivity | Human |
| Type | Monoclonal |
| Purity | Ammonium sulphate purified |
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP * | 5 μg/ChIP | Fig 1 |
| Western Blotting | 7 μg/ml | Fig 2 |
| Immunochemistry | 15 μg/ml | Fig 3 |
Figure 1. ChIP using the Diagenode monoclonal antibody against hERα
from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.
Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα
Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.
Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα
COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.
Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
| IC Immunochemistry Read more |
| Datasheet hERalpha MAb-009-050 DATASHEET Datasheet description | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: ER alpha Antibody (sample size) (Diagenode Cat# C15200009-10 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'product' => array( 'Product' => array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '103', 'name' => 'ER alpha monoclonal antibody', 'description' => 'Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '2.0 µg/µl', 'reactivity' => 'Human', 'type' => 'Monoclonal', 'purity' => 'Ammonium sulphate purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP <sup>*</sup> </td> <td>5 μg/ChIP</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>7 μg/ml</td> <td>Fig 2</td> </tr> <tr> <td>Immunochemistry</td> <td>15 μg/ml</td> <td>Fig 3</td> </tr> </tbody> </table> <small><sup>*</sup> Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-14 10:03:25', 'created' => '0000-00-00 00:00:00', 'select_label' => '103 - ER alpha monoclonal antibody (001 - 2.0 µg/µl - Human - Ammonium sulphate purified - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/er-alpha-monoclonal-antibody-classic-50-ug-25-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.' $meta_title = 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode' $product = array( 'Product' => array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '103', 'name' => 'ER alpha monoclonal antibody', 'description' => 'Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '2.0 µg/µl', 'reactivity' => 'Human', 'type' => 'Monoclonal', 'purity' => 'Ammonium sulphate purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP <sup>*</sup> </td> <td>5 μg/ChIP</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>7 μg/ml</td> <td>Fig 2</td> </tr> <tr> <td>Immunochemistry</td> <td>15 μg/ml</td> <td>Fig 3</td> </tr> </tbody> </table> <small><sup>*</sup> Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-14 10:03:25', 'created' => '0000-00-00 00:00:00', 'select_label' => '103 - ER alpha monoclonal antibody (001 - 2.0 µg/µl - Human - Ammonium sulphate purified - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '20', 'name' => 'C15200009', 'product_id' => '1968', 'modified' => '2016-02-18 18:02:12', 'created' => '2016-02-18 18:02:12' ), 'Master' => array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:23', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '505', 'name' => 'Datasheet hERalpha MAb-009-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_hERalpha_MAb-009-050.pdf', 'slug' => 'datasheet-heralpha-mab-009-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1779', 'name' => 'product/antibodies/ab-chip-icon.png', 'alt' => 'Antibody ChIP icon', 'modified' => '2020-08-12 11:52:55', 'created' => '2018-03-15 15:52:35', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3363', 'name' => 'Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer', 'authors' => 'Adurthi S. et al.', 'description' => '<p>Oestrogen controls Foxp3 expression in regulatory T cells (T<sub>reg</sub> cells) via a mechanism thought to involve oestrogen receptor alpha (ERα), but the molecular basis and functional impact of ERα signalling in T<sub>reg</sub> cells remain unclear. We report that ERα ligand oestradiol (E2) is significantly increased in human cervical cancer (CxCa) tissues and tumour-infiltrating T<sub>reg</sub> cells (CD4<sup>+</sup>CD25<sup>hi</sup>CD127<sup>low</sup>), whereas blocking ERα with the antagonist ICI 182,780 abolishes FOXP3 expression and impairs the function of CxCa infiltrating T<sub>reg</sub> cells. Using a novel approach of co-immunoprecipitation with antibodies to E2 for capture, we identified binding of E2:ERα complexes to FOXP3 protein in CxCa-derived T<sub>reg</sub> cells. Chromatin immunoprecipitation analyses of male blood T<sub>reg</sub> cells revealed ERα occupancy at the FOXP3 promoter and conserved non-coding DNA elements 2 and 3. Accordingly, computational analyses of the enriched regions uncovered eight putative oestrogen response elements predicted to form a loop that can activate the FOXP3 promoter. Together, these data suggest that E2-mediated ERα signalling is critical for the sustenance of FOXP3 expression and T<sub>reg</sub> cell function in human CxCa via direct interaction of ERα with FOXP3 promoter. Overall, our work gives a molecular insight into ERα signalling and highlights a fundamental role of E2 in controlling human T<sub>reg</sub> cell physiology.</p>', 'date' => '2017-12-11', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/29229929', 'doi' => '', 'modified' => '2018-04-24 10:07:53', 'created' => '2018-04-24 10:07:53', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '576', 'name' => 'ER alpha antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2020-07-01 14:59:24', 'created' => '2020-07-01 14:59:24', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '574', 'name' => 'ER alpha antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2020-07-01 14:58:20', 'created' => '2020-07-01 14:58:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '569', 'name' => 'ER alpha antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:55:44', 'created' => '2020-07-01 14:55:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '573', 'name' => 'ER alpha antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2020-07-01 14:57:51', 'created' => '2020-07-01 14:57:51', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '572', 'name' => 'ER alpha antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2020-07-01 14:57:14', 'created' => '2020-07-01 14:57:14', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '571', 'name' => 'ER alpha antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2020-07-01 14:56:44', 'created' => '2020-07-01 14:56:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '575', 'name' => 'ER alpha antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-JP-ja-GHS_2_0.pdf', 'countries' => 'JP', 'modified' => '2020-07-01 14:58:55', 'created' => '2020-07-01 14:58:55', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '570', 'name' => 'ER alpha antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:56:20', 'created' => '2020-07-01 14:56:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/er-alpha-monoclonal-antibody-classic-50-ug-25-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:23', 'created' => '2015-06-29 14:08:20' ) ) $pro = array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '27', 'product_id' => '1969', 'group_id' => '20' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(MAb-009-050)</span>' $country_code = 'US' $other_format = array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:23', 'created' => '2015-06-29 14:08:20' ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'ProductsApplication' => array( 'id' => '5057', 'product_id' => '1969', 'application_id' => '31' ) ) $slugs = array( (int) 0 => 'immunochemistry' ) $applications = array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'locale' => 'eng' ) $description = '<p>Immunochemistry</p>' $name = 'IC' $document = array( 'id' => '505', 'name' => 'Datasheet hERalpha MAb-009-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_hERalpha_MAb-009-050.pdf', 'slug' => 'datasheet-heralpha-mab-009-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '283', 'product_id' => '1969', 'document_id' => '505' ) ) $sds = array( 'id' => '570', 'name' => 'ER alpha antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:56:20', 'created' => '2020-07-01 14:56:20', 'ProductsSafetySheet' => array( 'id' => '1099', 'product_id' => '1969', 'safety_sheet_id' => '570' ) ) $publication = array( 'id' => '3363', 'name' => 'Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer', 'authors' => 'Adurthi S. et al.', 'description' => '<p>Oestrogen controls Foxp3 expression in regulatory T cells (T<sub>reg</sub> cells) via a mechanism thought to involve oestrogen receptor alpha (ERα), but the molecular basis and functional impact of ERα signalling in T<sub>reg</sub> cells remain unclear. We report that ERα ligand oestradiol (E2) is significantly increased in human cervical cancer (CxCa) tissues and tumour-infiltrating T<sub>reg</sub> cells (CD4<sup>+</sup>CD25<sup>hi</sup>CD127<sup>low</sup>), whereas blocking ERα with the antagonist ICI 182,780 abolishes FOXP3 expression and impairs the function of CxCa infiltrating T<sub>reg</sub> cells. Using a novel approach of co-immunoprecipitation with antibodies to E2 for capture, we identified binding of E2:ERα complexes to FOXP3 protein in CxCa-derived T<sub>reg</sub> cells. Chromatin immunoprecipitation analyses of male blood T<sub>reg</sub> cells revealed ERα occupancy at the FOXP3 promoter and conserved non-coding DNA elements 2 and 3. Accordingly, computational analyses of the enriched regions uncovered eight putative oestrogen response elements predicted to form a loop that can activate the FOXP3 promoter. Together, these data suggest that E2-mediated ERα signalling is critical for the sustenance of FOXP3 expression and T<sub>reg</sub> cell function in human CxCa via direct interaction of ERα with FOXP3 promoter. Overall, our work gives a molecular insight into ERα signalling and highlights a fundamental role of E2 in controlling human T<sub>reg</sub> cell physiology.</p>', 'date' => '2017-12-11', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/29229929', 'doi' => '', 'modified' => '2018-04-24 10:07:53', 'created' => '2018-04-24 10:07:53', 'ProductsPublication' => array( 'id' => '2735', 'product_id' => '1969', 'publication_id' => '3363' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/29229929" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'product' => array( 'Product' => array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '103', 'name' => 'ER alpha monoclonal antibody', 'description' => 'Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '2.0 µg/µl', 'reactivity' => 'Human', 'type' => 'Monoclonal', 'purity' => 'Ammonium sulphate purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP <sup>*</sup> </td> <td>5 μg/ChIP</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>7 μg/ml</td> <td>Fig 2</td> </tr> <tr> <td>Immunochemistry</td> <td>15 μg/ml</td> <td>Fig 3</td> </tr> </tbody> </table> <small><sup>*</sup> Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-14 10:03:25', 'created' => '0000-00-00 00:00:00', 'select_label' => '103 - ER alpha monoclonal antibody (001 - 2.0 µg/µl - Human - Ammonium sulphate purified - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/er-alpha-monoclonal-antibody-classic-50-ug-25-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.' $meta_title = 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode' $product = array( 'Product' => array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '103', 'name' => 'ER alpha monoclonal antibody', 'description' => 'Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '2.0 µg/µl', 'reactivity' => 'Human', 'type' => 'Monoclonal', 'purity' => 'Ammonium sulphate purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP <sup>*</sup> </td> <td>5 μg/ChIP</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>7 μg/ml</td> <td>Fig 2</td> </tr> <tr> <td>Immunochemistry</td> <td>15 μg/ml</td> <td>Fig 3</td> </tr> </tbody> </table> <small><sup>*</sup> Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-14 10:03:25', 'created' => '0000-00-00 00:00:00', 'select_label' => '103 - ER alpha monoclonal antibody (001 - 2.0 µg/µl - Human - Ammonium sulphate purified - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '20', 'name' => 'C15200009', 'product_id' => '1968', 'modified' => '2016-02-18 18:02:12', 'created' => '2016-02-18 18:02:12' ), 'Master' => array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:23', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '505', 'name' => 'Datasheet hERalpha MAb-009-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_hERalpha_MAb-009-050.pdf', 'slug' => 'datasheet-heralpha-mab-009-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1779', 'name' => 'product/antibodies/ab-chip-icon.png', 'alt' => 'Antibody ChIP icon', 'modified' => '2020-08-12 11:52:55', 'created' => '2018-03-15 15:52:35', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3363', 'name' => 'Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer', 'authors' => 'Adurthi S. et al.', 'description' => '<p>Oestrogen controls Foxp3 expression in regulatory T cells (T<sub>reg</sub> cells) via a mechanism thought to involve oestrogen receptor alpha (ERα), but the molecular basis and functional impact of ERα signalling in T<sub>reg</sub> cells remain unclear. We report that ERα ligand oestradiol (E2) is significantly increased in human cervical cancer (CxCa) tissues and tumour-infiltrating T<sub>reg</sub> cells (CD4<sup>+</sup>CD25<sup>hi</sup>CD127<sup>low</sup>), whereas blocking ERα with the antagonist ICI 182,780 abolishes FOXP3 expression and impairs the function of CxCa infiltrating T<sub>reg</sub> cells. Using a novel approach of co-immunoprecipitation with antibodies to E2 for capture, we identified binding of E2:ERα complexes to FOXP3 protein in CxCa-derived T<sub>reg</sub> cells. Chromatin immunoprecipitation analyses of male blood T<sub>reg</sub> cells revealed ERα occupancy at the FOXP3 promoter and conserved non-coding DNA elements 2 and 3. Accordingly, computational analyses of the enriched regions uncovered eight putative oestrogen response elements predicted to form a loop that can activate the FOXP3 promoter. Together, these data suggest that E2-mediated ERα signalling is critical for the sustenance of FOXP3 expression and T<sub>reg</sub> cell function in human CxCa via direct interaction of ERα with FOXP3 promoter. Overall, our work gives a molecular insight into ERα signalling and highlights a fundamental role of E2 in controlling human T<sub>reg</sub> cell physiology.</p>', 'date' => '2017-12-11', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/29229929', 'doi' => '', 'modified' => '2018-04-24 10:07:53', 'created' => '2018-04-24 10:07:53', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '576', 'name' => 'ER alpha antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2020-07-01 14:59:24', 'created' => '2020-07-01 14:59:24', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '574', 'name' => 'ER alpha antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2020-07-01 14:58:20', 'created' => '2020-07-01 14:58:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '569', 'name' => 'ER alpha antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:55:44', 'created' => '2020-07-01 14:55:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '573', 'name' => 'ER alpha antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2020-07-01 14:57:51', 'created' => '2020-07-01 14:57:51', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '572', 'name' => 'ER alpha antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2020-07-01 14:57:14', 'created' => '2020-07-01 14:57:14', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '571', 'name' => 'ER alpha antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2020-07-01 14:56:44', 'created' => '2020-07-01 14:56:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '575', 'name' => 'ER alpha antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-JP-ja-GHS_2_0.pdf', 'countries' => 'JP', 'modified' => '2020-07-01 14:58:55', 'created' => '2020-07-01 14:58:55', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '570', 'name' => 'ER alpha antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:56:20', 'created' => '2020-07-01 14:56:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/er-alpha-monoclonal-antibody-classic-50-ug-25-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:23', 'created' => '2015-06-29 14:08:20' ) ) $pro = array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '27', 'product_id' => '1969', 'group_id' => '20' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(MAb-009-050)</span>' $country_code = 'US' $other_format = array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:23', 'created' => '2015-06-29 14:08:20' ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'ProductsApplication' => array( 'id' => '5057', 'product_id' => '1969', 'application_id' => '31' ) ) $slugs = array( (int) 0 => 'immunochemistry' ) $applications = array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'locale' => 'eng' ) $description = '<p>Immunochemistry</p>' $name = 'IC' $document = array( 'id' => '505', 'name' => 'Datasheet hERalpha MAb-009-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_hERalpha_MAb-009-050.pdf', 'slug' => 'datasheet-heralpha-mab-009-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '283', 'product_id' => '1969', 'document_id' => '505' ) ) $sds = array( 'id' => '570', 'name' => 'ER alpha antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:56:20', 'created' => '2020-07-01 14:56:20', 'ProductsSafetySheet' => array( 'id' => '1099', 'product_id' => '1969', 'safety_sheet_id' => '570' ) ) $publication = array( 'id' => '3363', 'name' => 'Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer', 'authors' => 'Adurthi S. et al.', 'description' => '<p>Oestrogen controls Foxp3 expression in regulatory T cells (T<sub>reg</sub> cells) via a mechanism thought to involve oestrogen receptor alpha (ERα), but the molecular basis and functional impact of ERα signalling in T<sub>reg</sub> cells remain unclear. We report that ERα ligand oestradiol (E2) is significantly increased in human cervical cancer (CxCa) tissues and tumour-infiltrating T<sub>reg</sub> cells (CD4<sup>+</sup>CD25<sup>hi</sup>CD127<sup>low</sup>), whereas blocking ERα with the antagonist ICI 182,780 abolishes FOXP3 expression and impairs the function of CxCa infiltrating T<sub>reg</sub> cells. Using a novel approach of co-immunoprecipitation with antibodies to E2 for capture, we identified binding of E2:ERα complexes to FOXP3 protein in CxCa-derived T<sub>reg</sub> cells. Chromatin immunoprecipitation analyses of male blood T<sub>reg</sub> cells revealed ERα occupancy at the FOXP3 promoter and conserved non-coding DNA elements 2 and 3. Accordingly, computational analyses of the enriched regions uncovered eight putative oestrogen response elements predicted to form a loop that can activate the FOXP3 promoter. Together, these data suggest that E2-mediated ERα signalling is critical for the sustenance of FOXP3 expression and T<sub>reg</sub> cell function in human CxCa via direct interaction of ERα with FOXP3 promoter. Overall, our work gives a molecular insight into ERα signalling and highlights a fundamental role of E2 in controlling human T<sub>reg</sub> cell physiology.</p>', 'date' => '2017-12-11', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/29229929', 'doi' => '', 'modified' => '2018-04-24 10:07:53', 'created' => '2018-04-24 10:07:53', 'ProductsPublication' => array( 'id' => '2735', 'product_id' => '1969', 'publication_id' => '3363' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/29229929" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'product' => array( 'Product' => array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '103', 'name' => 'ER alpha monoclonal antibody', 'description' => 'Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '2.0 µg/µl', 'reactivity' => 'Human', 'type' => 'Monoclonal', 'purity' => 'Ammonium sulphate purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP <sup>*</sup> </td> <td>5 μg/ChIP</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>7 μg/ml</td> <td>Fig 2</td> </tr> <tr> <td>Immunochemistry</td> <td>15 μg/ml</td> <td>Fig 3</td> </tr> </tbody> </table> <small><sup>*</sup> Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-14 10:03:25', 'created' => '0000-00-00 00:00:00', 'select_label' => '103 - ER alpha monoclonal antibody (001 - 2.0 µg/µl - Human - Ammonium sulphate purified - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/er-alpha-monoclonal-antibody-classic-50-ug-25-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.' $meta_title = 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode' $product = array( 'Product' => array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '103', 'name' => 'ER alpha monoclonal antibody', 'description' => 'Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '2.0 µg/µl', 'reactivity' => 'Human', 'type' => 'Monoclonal', 'purity' => 'Ammonium sulphate purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP <sup>*</sup> </td> <td>5 μg/ChIP</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>7 μg/ml</td> <td>Fig 2</td> </tr> <tr> <td>Immunochemistry</td> <td>15 μg/ml</td> <td>Fig 3</td> </tr> </tbody> </table> <small><sup>*</sup> Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-14 10:03:25', 'created' => '0000-00-00 00:00:00', 'select_label' => '103 - ER alpha monoclonal antibody (001 - 2.0 µg/µl - Human - Ammonium sulphate purified - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '20', 'name' => 'C15200009', 'product_id' => '1968', 'modified' => '2016-02-18 18:02:12', 'created' => '2016-02-18 18:02:12' ), 'Master' => array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:23', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '505', 'name' => 'Datasheet hERalpha MAb-009-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_hERalpha_MAb-009-050.pdf', 'slug' => 'datasheet-heralpha-mab-009-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1779', 'name' => 'product/antibodies/ab-chip-icon.png', 'alt' => 'Antibody ChIP icon', 'modified' => '2020-08-12 11:52:55', 'created' => '2018-03-15 15:52:35', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3363', 'name' => 'Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer', 'authors' => 'Adurthi S. et al.', 'description' => '<p>Oestrogen controls Foxp3 expression in regulatory T cells (T<sub>reg</sub> cells) via a mechanism thought to involve oestrogen receptor alpha (ERα), but the molecular basis and functional impact of ERα signalling in T<sub>reg</sub> cells remain unclear. We report that ERα ligand oestradiol (E2) is significantly increased in human cervical cancer (CxCa) tissues and tumour-infiltrating T<sub>reg</sub> cells (CD4<sup>+</sup>CD25<sup>hi</sup>CD127<sup>low</sup>), whereas blocking ERα with the antagonist ICI 182,780 abolishes FOXP3 expression and impairs the function of CxCa infiltrating T<sub>reg</sub> cells. Using a novel approach of co-immunoprecipitation with antibodies to E2 for capture, we identified binding of E2:ERα complexes to FOXP3 protein in CxCa-derived T<sub>reg</sub> cells. Chromatin immunoprecipitation analyses of male blood T<sub>reg</sub> cells revealed ERα occupancy at the FOXP3 promoter and conserved non-coding DNA elements 2 and 3. Accordingly, computational analyses of the enriched regions uncovered eight putative oestrogen response elements predicted to form a loop that can activate the FOXP3 promoter. Together, these data suggest that E2-mediated ERα signalling is critical for the sustenance of FOXP3 expression and T<sub>reg</sub> cell function in human CxCa via direct interaction of ERα with FOXP3 promoter. Overall, our work gives a molecular insight into ERα signalling and highlights a fundamental role of E2 in controlling human T<sub>reg</sub> cell physiology.</p>', 'date' => '2017-12-11', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/29229929', 'doi' => '', 'modified' => '2018-04-24 10:07:53', 'created' => '2018-04-24 10:07:53', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '576', 'name' => 'ER alpha antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2020-07-01 14:59:24', 'created' => '2020-07-01 14:59:24', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '574', 'name' => 'ER alpha antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2020-07-01 14:58:20', 'created' => '2020-07-01 14:58:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '569', 'name' => 'ER alpha antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:55:44', 'created' => '2020-07-01 14:55:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '573', 'name' => 'ER alpha antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2020-07-01 14:57:51', 'created' => '2020-07-01 14:57:51', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '572', 'name' => 'ER alpha antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2020-07-01 14:57:14', 'created' => '2020-07-01 14:57:14', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '571', 'name' => 'ER alpha antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2020-07-01 14:56:44', 'created' => '2020-07-01 14:56:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '575', 'name' => 'ER alpha antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-JP-ja-GHS_2_0.pdf', 'countries' => 'JP', 'modified' => '2020-07-01 14:58:55', 'created' => '2020-07-01 14:58:55', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '570', 'name' => 'ER alpha antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:56:20', 'created' => '2020-07-01 14:56:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/er-alpha-monoclonal-antibody-classic-50-ug-25-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:23', 'created' => '2015-06-29 14:08:20' ) ) $pro = array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '27', 'product_id' => '1969', 'group_id' => '20' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(MAb-009-050)</span>' $country_code = 'US' $other_format = array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:23', 'created' => '2015-06-29 14:08:20' ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'ProductsApplication' => array( 'id' => '5057', 'product_id' => '1969', 'application_id' => '31' ) ) $slugs = array( (int) 0 => 'immunochemistry' ) $applications = array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'locale' => 'eng' ) $description = '<p>Immunochemistry</p>' $name = 'IC' $document = array( 'id' => '505', 'name' => 'Datasheet hERalpha MAb-009-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_hERalpha_MAb-009-050.pdf', 'slug' => 'datasheet-heralpha-mab-009-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '283', 'product_id' => '1969', 'document_id' => '505' ) ) $sds = array( 'id' => '570', 'name' => 'ER alpha antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:56:20', 'created' => '2020-07-01 14:56:20', 'ProductsSafetySheet' => array( 'id' => '1099', 'product_id' => '1969', 'safety_sheet_id' => '570' ) ) $publication = array( 'id' => '3363', 'name' => 'Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer', 'authors' => 'Adurthi S. et al.', 'description' => '<p>Oestrogen controls Foxp3 expression in regulatory T cells (T<sub>reg</sub> cells) via a mechanism thought to involve oestrogen receptor alpha (ERα), but the molecular basis and functional impact of ERα signalling in T<sub>reg</sub> cells remain unclear. We report that ERα ligand oestradiol (E2) is significantly increased in human cervical cancer (CxCa) tissues and tumour-infiltrating T<sub>reg</sub> cells (CD4<sup>+</sup>CD25<sup>hi</sup>CD127<sup>low</sup>), whereas blocking ERα with the antagonist ICI 182,780 abolishes FOXP3 expression and impairs the function of CxCa infiltrating T<sub>reg</sub> cells. Using a novel approach of co-immunoprecipitation with antibodies to E2 for capture, we identified binding of E2:ERα complexes to FOXP3 protein in CxCa-derived T<sub>reg</sub> cells. Chromatin immunoprecipitation analyses of male blood T<sub>reg</sub> cells revealed ERα occupancy at the FOXP3 promoter and conserved non-coding DNA elements 2 and 3. Accordingly, computational analyses of the enriched regions uncovered eight putative oestrogen response elements predicted to form a loop that can activate the FOXP3 promoter. Together, these data suggest that E2-mediated ERα signalling is critical for the sustenance of FOXP3 expression and T<sub>reg</sub> cell function in human CxCa via direct interaction of ERα with FOXP3 promoter. Overall, our work gives a molecular insight into ERα signalling and highlights a fundamental role of E2 in controlling human T<sub>reg</sub> cell physiology.</p>', 'date' => '2017-12-11', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/29229929', 'doi' => '', 'modified' => '2018-04-24 10:07:53', 'created' => '2018-04-24 10:07:53', 'ProductsPublication' => array( 'id' => '2735', 'product_id' => '1969', 'publication_id' => '3363' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/29229929" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'product' => array( 'Product' => array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '103', 'name' => 'ER alpha monoclonal antibody', 'description' => 'Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '2.0 µg/µl', 'reactivity' => 'Human', 'type' => 'Monoclonal', 'purity' => 'Ammonium sulphate purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP <sup>*</sup> </td> <td>5 μg/ChIP</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>7 μg/ml</td> <td>Fig 2</td> </tr> <tr> <td>Immunochemistry</td> <td>15 μg/ml</td> <td>Fig 3</td> </tr> </tbody> </table> <small><sup>*</sup> Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-14 10:03:25', 'created' => '0000-00-00 00:00:00', 'select_label' => '103 - ER alpha monoclonal antibody (001 - 2.0 µg/µl - Human - Ammonium sulphate purified - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/er-alpha-monoclonal-antibody-classic-50-ug-25-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.' $meta_title = 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode' $product = array( 'Product' => array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '103', 'name' => 'ER alpha monoclonal antibody', 'description' => 'Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '2.0 µg/µl', 'reactivity' => 'Human', 'type' => 'Monoclonal', 'purity' => 'Ammonium sulphate purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP <sup>*</sup> </td> <td>5 μg/ChIP</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>7 μg/ml</td> <td>Fig 2</td> </tr> <tr> <td>Immunochemistry</td> <td>15 μg/ml</td> <td>Fig 3</td> </tr> </tbody> </table> <small><sup>*</sup> Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-14 10:03:25', 'created' => '0000-00-00 00:00:00', 'select_label' => '103 - ER alpha monoclonal antibody (001 - 2.0 µg/µl - Human - Ammonium sulphate purified - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '20', 'name' => 'C15200009', 'product_id' => '1968', 'modified' => '2016-02-18 18:02:12', 'created' => '2016-02-18 18:02:12' ), 'Master' => array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:23', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '505', 'name' => 'Datasheet hERalpha MAb-009-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_hERalpha_MAb-009-050.pdf', 'slug' => 'datasheet-heralpha-mab-009-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1779', 'name' => 'product/antibodies/ab-chip-icon.png', 'alt' => 'Antibody ChIP icon', 'modified' => '2020-08-12 11:52:55', 'created' => '2018-03-15 15:52:35', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3363', 'name' => 'Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer', 'authors' => 'Adurthi S. et al.', 'description' => '<p>Oestrogen controls Foxp3 expression in regulatory T cells (T<sub>reg</sub> cells) via a mechanism thought to involve oestrogen receptor alpha (ERα), but the molecular basis and functional impact of ERα signalling in T<sub>reg</sub> cells remain unclear. We report that ERα ligand oestradiol (E2) is significantly increased in human cervical cancer (CxCa) tissues and tumour-infiltrating T<sub>reg</sub> cells (CD4<sup>+</sup>CD25<sup>hi</sup>CD127<sup>low</sup>), whereas blocking ERα with the antagonist ICI 182,780 abolishes FOXP3 expression and impairs the function of CxCa infiltrating T<sub>reg</sub> cells. Using a novel approach of co-immunoprecipitation with antibodies to E2 for capture, we identified binding of E2:ERα complexes to FOXP3 protein in CxCa-derived T<sub>reg</sub> cells. Chromatin immunoprecipitation analyses of male blood T<sub>reg</sub> cells revealed ERα occupancy at the FOXP3 promoter and conserved non-coding DNA elements 2 and 3. Accordingly, computational analyses of the enriched regions uncovered eight putative oestrogen response elements predicted to form a loop that can activate the FOXP3 promoter. Together, these data suggest that E2-mediated ERα signalling is critical for the sustenance of FOXP3 expression and T<sub>reg</sub> cell function in human CxCa via direct interaction of ERα with FOXP3 promoter. Overall, our work gives a molecular insight into ERα signalling and highlights a fundamental role of E2 in controlling human T<sub>reg</sub> cell physiology.</p>', 'date' => '2017-12-11', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/29229929', 'doi' => '', 'modified' => '2018-04-24 10:07:53', 'created' => '2018-04-24 10:07:53', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '576', 'name' => 'ER alpha antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2020-07-01 14:59:24', 'created' => '2020-07-01 14:59:24', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '574', 'name' => 'ER alpha antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2020-07-01 14:58:20', 'created' => '2020-07-01 14:58:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '569', 'name' => 'ER alpha antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:55:44', 'created' => '2020-07-01 14:55:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '573', 'name' => 'ER alpha antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2020-07-01 14:57:51', 'created' => '2020-07-01 14:57:51', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '572', 'name' => 'ER alpha antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2020-07-01 14:57:14', 'created' => '2020-07-01 14:57:14', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '571', 'name' => 'ER alpha antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2020-07-01 14:56:44', 'created' => '2020-07-01 14:56:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '575', 'name' => 'ER alpha antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-JP-ja-GHS_2_0.pdf', 'countries' => 'JP', 'modified' => '2020-07-01 14:58:55', 'created' => '2020-07-01 14:58:55', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '570', 'name' => 'ER alpha antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:56:20', 'created' => '2020-07-01 14:56:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/er-alpha-monoclonal-antibody-classic-50-ug-25-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:23', 'created' => '2015-06-29 14:08:20' ) ) $pro = array( 'id' => '1969', 'antibody_id' => '103', 'name' => 'ER alpha Antibody (sample size)', 'description' => '<p>Alternative names: <span><strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_chip.png" alt="chip" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200009-10', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-sample-size-10-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, WB and ICC. Batch-specific data available on the website. Alternative names: ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:37', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '27', 'product_id' => '1969', 'group_id' => '20' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(MAb-009-050)</span>' $country_code = 'US' $other_format = array( 'id' => '1968', 'antibody_id' => '103', 'name' => 'ER alpha Antibody', 'description' => '<p>Alternative names:<strong>ESR</strong>, <strong>ESR1</strong>, <strong>ESRA</strong>, <strong>NR3A1</strong></p> <p><span>Monoclonal antibody raised in mouse against the NH2 terminus of the human <strong>ERα</strong> (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig1.png" alt="ER alpha Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/25 µl', 'catalog_number' => 'C15200009', 'old_catalog_number' => 'MAb-009-050', 'sf_code' => 'C15200009-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'er-alpha-monoclonal-antibody-classic-50-ug-25-ul', 'meta_title' => 'ER alpha Antibody - ChIP Grade (C15200009) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'ER alpha (Estrogen receptor alpha) Monoclonal Antibody validated in ChIP-qPCR, IC and WB. Batch-specific data available on the website. Alternative names:ESR, ESR1, ESRA, NR3A1. Sample size available.', 'modified' => '2021-12-23 12:07:23', 'created' => '2015-06-29 14:08:20' ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'ProductsApplication' => array( 'id' => '5057', 'product_id' => '1969', 'application_id' => '31' ) ) $slugs = array( (int) 0 => 'immunochemistry' ) $applications = array( 'id' => '31', 'position' => '10', 'parent_id' => '40', 'name' => 'IC', 'description' => '<p>Immunochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunochemistry', 'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications', 'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:31', 'created' => '2015-07-08 13:48:10', 'locale' => 'eng' ) $description = '<p>Immunochemistry</p>' $name = 'IC' $document = array( 'id' => '505', 'name' => 'Datasheet hERalpha MAb-009-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_hERalpha_MAb-009-050.pdf', 'slug' => 'datasheet-heralpha-mab-009-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '283', 'product_id' => '1969', 'document_id' => '505' ) ) $sds = array( 'id' => '570', 'name' => 'ER alpha antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 14:56:20', 'created' => '2020-07-01 14:56:20', 'ProductsSafetySheet' => array( 'id' => '1099', 'product_id' => '1969', 'safety_sheet_id' => '570' ) ) $publication = array( 'id' => '3363', 'name' => 'Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer', 'authors' => 'Adurthi S. et al.', 'description' => '<p>Oestrogen controls Foxp3 expression in regulatory T cells (T<sub>reg</sub> cells) via a mechanism thought to involve oestrogen receptor alpha (ERα), but the molecular basis and functional impact of ERα signalling in T<sub>reg</sub> cells remain unclear. We report that ERα ligand oestradiol (E2) is significantly increased in human cervical cancer (CxCa) tissues and tumour-infiltrating T<sub>reg</sub> cells (CD4<sup>+</sup>CD25<sup>hi</sup>CD127<sup>low</sup>), whereas blocking ERα with the antagonist ICI 182,780 abolishes FOXP3 expression and impairs the function of CxCa infiltrating T<sub>reg</sub> cells. Using a novel approach of co-immunoprecipitation with antibodies to E2 for capture, we identified binding of E2:ERα complexes to FOXP3 protein in CxCa-derived T<sub>reg</sub> cells. Chromatin immunoprecipitation analyses of male blood T<sub>reg</sub> cells revealed ERα occupancy at the FOXP3 promoter and conserved non-coding DNA elements 2 and 3. Accordingly, computational analyses of the enriched regions uncovered eight putative oestrogen response elements predicted to form a loop that can activate the FOXP3 promoter. Together, these data suggest that E2-mediated ERα signalling is critical for the sustenance of FOXP3 expression and T<sub>reg</sub> cell function in human CxCa via direct interaction of ERα with FOXP3 promoter. Overall, our work gives a molecular insight into ERα signalling and highlights a fundamental role of E2 in controlling human T<sub>reg</sub> cell physiology.</p>', 'date' => '2017-12-11', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/29229929', 'doi' => '', 'modified' => '2018-04-24 10:07:53', 'created' => '2018-04-24 10:07:53', 'ProductsPublication' => array( 'id' => '2735', 'product_id' => '1969', 'publication_id' => '3363' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/29229929" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
To ensure you see the information most relevant to you, please select your country. Please note that your browser will need to be configured to accept cookies.
Diagenode will process your personal data in strict accordance with its privacy policy. This will include sending you updates about us, our products, and resources we think would be of interest to you.