FLAG Antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

Antibody portal

Antibodies

Catalog Number

Format

Price

C15200209

100 µg

$440.00
Bulk order

Monoclonal antibody raised in mouse against Flag™ (peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)), using a KLH-conjugated synthetic peptide.

Lot	002
Concentration	1 μg/μl
Species reactivity	Carboxy and amino terminal linked FLAG™ tagged recombinant proteins
Type	Monoclonal
Purity	Protein A purified
Host	Mouse
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:20,000 - 1:100,000
Western Blotting	1:2,000 - 1:20,000	Fig 1, 2

Validation Data

Figure 1. FLAG antibody western blot results
Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 μL. Samples were diluted 10-fold, transferred to nitrocellulose membrane and WB was performed with the FLAG monoclonal antibody.

Figure 2. FLAG antibody western blot results
Monoclonal Antibody to detect FLAGTM conjugated proteins detects both C terminal linked and N terminal linked FLAGTM tagged recombinant proteins by western blot.

Documents
Datasheet FLAG C15200209 DATASHEET
Datasheet description Download

Publications

How to properly cite this product in your work

Diagenode strongly recommends using this: FLAG Antibody (Diagenode Cat# C15200209 Lot# 002). Click here to copy to clipboard.

Using our products in your publication? Let us know!

ThPOK is a critical multifaceted regulator of myeloid lineagedevelopment.
Basu J. et al.
The transcription factor ThPOK (encoded by Zbtb7b) is well known for its role as a master regulator of CD4 lineage commitment in the thymus. Here, we report an unexpected and critical role of ThPOK as a multifaceted regulator of myeloid lineage commitment, differentiation and maturation. Using reporter and knockout ...

Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => '',
	'meta_description' => 'FLAG Monoclonal Antibody  validated  in  WB and ELISA.  ',
	'meta_title' => 'FLAG Monoclonal Antibody | Diagenode',
	'product' => array(
		'Product' => array(
			'id' => '2019',
			'antibody_id' => '346',
			'name' => 'FLAG Antibody ',
			'description' => '<p>Monoclonal antibody raised in mouse against Flag&trade; (peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)), using a KLH-conjugated synthetic peptide.</p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200209-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="385" height="176" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 1. FLAG antibody western blot results </strong><br />Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 &mu;L. Samples were diluted 10-fold, transferred to nitrocellulose membrane and WB was performed with the FLAG monoclonal antibody.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200209-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="84" height="164" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 2. FLAG antibody western blot results </strong><br />Monoclonal Antibody to detect FLAGTM conjugated proteins detects both <g class="gr_ gr_14 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="14" data-gr-id="14">C terminal</g> linked and <g class="gr_ gr_15 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="15" data-gr-id="15">N terminal</g> linked FLAGTM tagged recombinant proteins by western blot.</small></p>
</div>
</div>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '100 µg',
			'catalog_number' => 'C15200209',
			'old_catalog_number' => '',
			'sf_code' => 'C15200209-D001-000526',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '435',
			'price_USD' => '440',
			'price_GBP' => '395',
			'price_JPY' => '68145',
			'price_CNY' => '',
			'price_AUD' => '1100',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'flag-monoclonal-antibody-classic-100-ug',
			'meta_title' => 'FLAG Monoclonal Antibody | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'FLAG Monoclonal Antibody  validated  in  WB and ELISA.  ',
			'modified' => '2022-01-05 15:36:13',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '346',
			'name' => 'FLAG monoclonal antibody - Classic',
			'description' => 'Antibody for the detection of FLAGTM recognizes FLAGTM and is optimally suited for monitoring the expression of FLAGTM tagged fusion proteins. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.',
			'clonality' => '',
			'isotype' => '',
			'lot' => '002',
			'concentration' => '1 μg/μl ',
			'reactivity' => 'Carboxy and amino terminal linked FLAG™ tagged recombinant proteins ',
			'type' => 'Monoclonal',
			'purity' => 'Protein A purified',
			'classification' => '',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:20,000 - 1:100,000</td>
<td></td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:2,000 - 1:20,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2023-05-04 10:48:01',
			'created' => '2015-07-16 16:57:09',
			'select_label' => '346 - FLAG monoclonal antibody - Classic (002 - 1 μg/μl  - Carboxy and amino terminal linked FLAG™ tagged recombinant proteins  - Protein A purified - Mouse)'
		),
		'Slave' => array(),
		'Group' => array(),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array()
	)
)
$language = 'en'
$meta_keywords = ''
$meta_description = 'FLAG Monoclonal Antibody  validated  in  WB and ELISA.  '
$meta_title = 'FLAG Monoclonal Antibody | Diagenode'
$product = array(
	'Product' => array(
		'id' => '2019',
		'antibody_id' => '346',
		'name' => 'FLAG Antibody ',
		'description' => '<p>Monoclonal antibody raised in mouse against Flag&trade; (peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)), using a KLH-conjugated synthetic peptide.</p>',
		'label1' => 'Validation Data',
		'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200209-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="385" height="176" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 1. FLAG antibody western blot results </strong><br />Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 &mu;L. Samples were diluted 10-fold, transferred to nitrocellulose membrane and WB was performed with the FLAG monoclonal antibody.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200209-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="84" height="164" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 2. FLAG antibody western blot results </strong><br />Monoclonal Antibody to detect FLAGTM conjugated proteins detects both <g class="gr_ gr_14 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="14" data-gr-id="14">C terminal</g> linked and <g class="gr_ gr_15 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="15" data-gr-id="15">N terminal</g> linked FLAGTM tagged recombinant proteins by western blot.</small></p>
</div>
</div>',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '100 µg',
		'catalog_number' => 'C15200209',
		'old_catalog_number' => '',
		'sf_code' => 'C15200209-D001-000526',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '435',
		'price_USD' => '440',
		'price_GBP' => '395',
		'price_JPY' => '68145',
		'price_CNY' => '',
		'price_AUD' => '1100',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'flag-monoclonal-antibody-classic-100-ug',
		'meta_title' => 'FLAG Monoclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'FLAG Monoclonal Antibody  validated  in  WB and ELISA.  ',
		'modified' => '2022-01-05 15:36:13',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'eng'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => '346',
		'name' => 'FLAG monoclonal antibody - Classic',
		'description' => 'Antibody for the detection of FLAGTM recognizes FLAGTM and is optimally suited for monitoring the expression of FLAGTM tagged fusion proteins. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.',
		'clonality' => '',
		'isotype' => '',
		'lot' => '002',
		'concentration' => '1 μg/μl ',
		'reactivity' => 'Carboxy and amino terminal linked FLAG™ tagged recombinant proteins ',
		'type' => 'Monoclonal',
		'purity' => 'Protein A purified',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:20,000 - 1:100,000</td>
<td></td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:2,000 - 1:20,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
		'storage_conditions' => '',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => '',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2023-05-04 10:48:01',
		'created' => '2015-07-16 16:57:09',
		'select_label' => '346 - FLAG monoclonal antibody - Classic (002 - 1 μg/μl  - Carboxy and amino terminal linked FLAG™ tagged recombinant proteins  - Protein A purified - Mouse)'
	),
	'Slave' => array(),
	'Group' => array(),
	'Related' => array(),
	'Application' => array(
		(int) 0 => array(
			'id' => '20',
			'position' => '10',
			'parent_id' => '40',
			'name' => 'ELISA',
			'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
</div>',
			'in_footer' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'slug' => 'elisa-antibodies',
			'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody',
			'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications',
			'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
			'modified' => '2016-01-13 12:21:41',
			'created' => '2014-07-08 08:13:28',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '19',
			'position' => '10',
			'parent_id' => '40',
			'name' => 'WB',
			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
			'in_footer' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'slug' => 'western-blot-antibodies',
			'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
			'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
			'modified' => '2016-04-26 12:44:51',
			'created' => '2015-01-07 09:20:00',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '32',
			'position' => '50',
			'parent_id' => '4',
			'name' => 'Epitope tags',
			'description' => '',
			'no_promo' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'protein-tag',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Protein tag,Polyclonal antibody,Monoclonal Protein tag antibodies',
			'meta_description' => 'Diagenode offers Polyclonal and Monoclonal Protein tag Antibodies',
			'meta_title' => 'Polyclonal and Monoclonal Protein tag Antibodies | Diagenode',
			'modified' => '2019-07-04 16:02:49',
			'created' => '2015-05-09 11:35:10',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		),
		(int) 1 => array(
			'id' => '103',
			'position' => '0',
			'parent_id' => '4',
			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'all-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
			'modified' => '2019-07-03 10:55:44',
			'created' => '2015-11-02 14:49:22',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '568',
			'name' => 'Datasheet FLAG C15200209',
			'description' => 'Datasheet description',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/antibodies/Datasheet_FLAG_C15200209.pdf',
			'slug' => 'datasheet-flag-c15200209',
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => '2015-07-07 11:47:44',
			'created' => '2015-07-07 11:47:44',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '250',
			'name' => 'product/antibodies/antibody.png',
			'alt' => 'Mouse IgG',
			'modified' => '2020-11-27 07:00:09',
			'created' => '2015-07-17 10:12:18',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '4828',
			'name' => 'ThPOK is a critical multifaceted regulator of myeloid lineagedevelopment.',
			'authors' => 'Basu  J. et al.',
			'description' => '<p>The transcription factor ThPOK (encoded by Zbtb7b) is well known for its role as a master regulator of CD4 lineage commitment in the thymus. Here, we report an unexpected and critical role of ThPOK as a multifaceted regulator of myeloid lineage commitment, differentiation and maturation. Using reporter and knockout mouse models combined with single-cell RNA-sequencing, progenitor transfer and colony assays, we show that ThPOK controls monocyte-dendritic cell versus granulocyte lineage production during homeostatic differentiation, and serves as a brake for neutrophil maturation in granulocyte lineage-specified cells through transcriptional regulation of lineage-specific transcription factors and RNA via altered messenger RNA splicing to reprogram intron retention.</p>',
			'date' => '2023-08-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37474652',
			'doi' => '10.1038/s41590-023-01549-3',
			'modified' => '2023-08-01 13:37:22',
			'created' => '2023-08-01 15:59:38',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array()
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'ProductsApplication' => array(
		'id' => '2176',
		'product_id' => '2019',
		'application_id' => '19'
	)
)
$slugs = array(
	(int) 0 => 'western-blot-antibodies'
)
$applications = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'locale' => 'eng'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
	'id' => '568',
	'name' => 'Datasheet FLAG C15200209',
	'description' => 'Datasheet description',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/antibodies/Datasheet_FLAG_C15200209.pdf',
	'slug' => 'datasheet-flag-c15200209',
	'meta_keywords' => null,
	'meta_description' => null,
	'modified' => '2015-07-07 11:47:44',
	'created' => '2015-07-07 11:47:44',
	'ProductsDocument' => array(
		'id' => '294',
		'product_id' => '2019',
		'document_id' => '568'
	)
)
$publication = array(
	'id' => '4828',
	'name' => 'ThPOK is a critical multifaceted regulator of myeloid lineagedevelopment.',
	'authors' => 'Basu  J. et al.',
	'description' => '<p>The transcription factor ThPOK (encoded by Zbtb7b) is well known for its role as a master regulator of CD4 lineage commitment in the thymus. Here, we report an unexpected and critical role of ThPOK as a multifaceted regulator of myeloid lineage commitment, differentiation and maturation. Using reporter and knockout mouse models combined with single-cell RNA-sequencing, progenitor transfer and colony assays, we show that ThPOK controls monocyte-dendritic cell versus granulocyte lineage production during homeostatic differentiation, and serves as a brake for neutrophil maturation in granulocyte lineage-specified cells through transcriptional regulation of lineage-specific transcription factors and RNA via altered messenger RNA splicing to reprogram intron retention.</p>',
	'date' => '2023-08-01',
	'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37474652',
	'doi' => '10.1038/s41590-023-01549-3',
	'modified' => '2023-08-01 13:37:22',
	'created' => '2023-08-01 15:59:38',
	'ProductsPublication' => array(
		'id' => '7029',
		'product_id' => '2019',
		'publication_id' => '4828'
	)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/37474652" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => '',
	'meta_description' => 'FLAG Monoclonal Antibody  validated  in  WB and ELISA.  ',
	'meta_title' => 'FLAG Monoclonal Antibody | Diagenode',
	'product' => array(
		'Product' => array(
			'id' => '2019',
			'antibody_id' => '346',
			'name' => 'FLAG Antibody ',
			'description' => '<p>Monoclonal antibody raised in mouse against Flag&trade; (peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)), using a KLH-conjugated synthetic peptide.</p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200209-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="385" height="176" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 1. FLAG antibody western blot results </strong><br />Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 &mu;L. Samples were diluted 10-fold, transferred to nitrocellulose membrane and WB was performed with the FLAG monoclonal antibody.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200209-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="84" height="164" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 2. FLAG antibody western blot results </strong><br />Monoclonal Antibody to detect FLAGTM conjugated proteins detects both <g class="gr_ gr_14 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="14" data-gr-id="14">C terminal</g> linked and <g class="gr_ gr_15 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="15" data-gr-id="15">N terminal</g> linked FLAGTM tagged recombinant proteins by western blot.</small></p>
</div>
</div>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '100 µg',
			'catalog_number' => 'C15200209',
			'old_catalog_number' => '',
			'sf_code' => 'C15200209-D001-000526',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '435',
			'price_USD' => '440',
			'price_GBP' => '395',
			'price_JPY' => '68145',
			'price_CNY' => '',
			'price_AUD' => '1100',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'flag-monoclonal-antibody-classic-100-ug',
			'meta_title' => 'FLAG Monoclonal Antibody | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'FLAG Monoclonal Antibody  validated  in  WB and ELISA.  ',
			'modified' => '2022-01-05 15:36:13',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '346',
			'name' => 'FLAG monoclonal antibody - Classic',
			'description' => 'Antibody for the detection of FLAGTM recognizes FLAGTM and is optimally suited for monitoring the expression of FLAGTM tagged fusion proteins. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.',
			'clonality' => '',
			'isotype' => '',
			'lot' => '002',
			'concentration' => '1 μg/μl ',
			'reactivity' => 'Carboxy and amino terminal linked FLAG™ tagged recombinant proteins ',
			'type' => 'Monoclonal',
			'purity' => 'Protein A purified',
			'classification' => '',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:20,000 - 1:100,000</td>
<td></td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:2,000 - 1:20,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2023-05-04 10:48:01',
			'created' => '2015-07-16 16:57:09',
			'select_label' => '346 - FLAG monoclonal antibody - Classic (002 - 1 μg/μl  - Carboxy and amino terminal linked FLAG™ tagged recombinant proteins  - Protein A purified - Mouse)'
		),
		'Slave' => array(),
		'Group' => array(),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array()
	)
)
$language = 'en'
$meta_keywords = ''
$meta_description = 'FLAG Monoclonal Antibody  validated  in  WB and ELISA.  '
$meta_title = 'FLAG Monoclonal Antibody | Diagenode'
$product = array(
	'Product' => array(
		'id' => '2019',
		'antibody_id' => '346',
		'name' => 'FLAG Antibody ',
		'description' => '<p>Monoclonal antibody raised in mouse against Flag&trade; (peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)), using a KLH-conjugated synthetic peptide.</p>',
		'label1' => 'Validation Data',
		'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200209-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="385" height="176" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 1. FLAG antibody western blot results </strong><br />Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 &mu;L. Samples were diluted 10-fold, transferred to nitrocellulose membrane and WB was performed with the FLAG monoclonal antibody.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200209-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="84" height="164" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 2. FLAG antibody western blot results </strong><br />Monoclonal Antibody to detect FLAGTM conjugated proteins detects both <g class="gr_ gr_14 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="14" data-gr-id="14">C terminal</g> linked and <g class="gr_ gr_15 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="15" data-gr-id="15">N terminal</g> linked FLAGTM tagged recombinant proteins by western blot.</small></p>
</div>
</div>',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '100 µg',
		'catalog_number' => 'C15200209',
		'old_catalog_number' => '',
		'sf_code' => 'C15200209-D001-000526',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '435',
		'price_USD' => '440',
		'price_GBP' => '395',
		'price_JPY' => '68145',
		'price_CNY' => '',
		'price_AUD' => '1100',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'flag-monoclonal-antibody-classic-100-ug',
		'meta_title' => 'FLAG Monoclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'FLAG Monoclonal Antibody  validated  in  WB and ELISA.  ',
		'modified' => '2022-01-05 15:36:13',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'eng'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => '346',
		'name' => 'FLAG monoclonal antibody - Classic',
		'description' => 'Antibody for the detection of FLAGTM recognizes FLAGTM and is optimally suited for monitoring the expression of FLAGTM tagged fusion proteins. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.',
		'clonality' => '',
		'isotype' => '',
		'lot' => '002',
		'concentration' => '1 μg/μl ',
		'reactivity' => 'Carboxy and amino terminal linked FLAG™ tagged recombinant proteins ',
		'type' => 'Monoclonal',
		'purity' => 'Protein A purified',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:20,000 - 1:100,000</td>
<td></td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:2,000 - 1:20,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
		'storage_conditions' => '',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => '',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2023-05-04 10:48:01',
		'created' => '2015-07-16 16:57:09',
		'select_label' => '346 - FLAG monoclonal antibody - Classic (002 - 1 μg/μl  - Carboxy and amino terminal linked FLAG™ tagged recombinant proteins  - Protein A purified - Mouse)'
	),
	'Slave' => array(),
	'Group' => array(),
	'Related' => array(),
	'Application' => array(
		(int) 0 => array(
			'id' => '20',
			'position' => '10',
			'parent_id' => '40',
			'name' => 'ELISA',
			'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
</div>',
			'in_footer' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'slug' => 'elisa-antibodies',
			'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody',
			'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications',
			'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
			'modified' => '2016-01-13 12:21:41',
			'created' => '2014-07-08 08:13:28',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '19',
			'position' => '10',
			'parent_id' => '40',
			'name' => 'WB',
			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
			'in_footer' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'slug' => 'western-blot-antibodies',
			'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
			'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
			'modified' => '2016-04-26 12:44:51',
			'created' => '2015-01-07 09:20:00',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '32',
			'position' => '50',
			'parent_id' => '4',
			'name' => 'Epitope tags',
			'description' => '',
			'no_promo' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'protein-tag',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Protein tag,Polyclonal antibody,Monoclonal Protein tag antibodies',
			'meta_description' => 'Diagenode offers Polyclonal and Monoclonal Protein tag Antibodies',
			'meta_title' => 'Polyclonal and Monoclonal Protein tag Antibodies | Diagenode',
			'modified' => '2019-07-04 16:02:49',
			'created' => '2015-05-09 11:35:10',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		),
		(int) 1 => array(
			'id' => '103',
			'position' => '0',
			'parent_id' => '4',
			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'all-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
			'modified' => '2019-07-03 10:55:44',
			'created' => '2015-11-02 14:49:22',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '568',
			'name' => 'Datasheet FLAG C15200209',
			'description' => 'Datasheet description',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/antibodies/Datasheet_FLAG_C15200209.pdf',
			'slug' => 'datasheet-flag-c15200209',
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => '2015-07-07 11:47:44',
			'created' => '2015-07-07 11:47:44',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '250',
			'name' => 'product/antibodies/antibody.png',
			'alt' => 'Mouse IgG',
			'modified' => '2020-11-27 07:00:09',
			'created' => '2015-07-17 10:12:18',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '4828',
			'name' => 'ThPOK is a critical multifaceted regulator of myeloid lineagedevelopment.',
			'authors' => 'Basu  J. et al.',
			'description' => '<p>The transcription factor ThPOK (encoded by Zbtb7b) is well known for its role as a master regulator of CD4 lineage commitment in the thymus. Here, we report an unexpected and critical role of ThPOK as a multifaceted regulator of myeloid lineage commitment, differentiation and maturation. Using reporter and knockout mouse models combined with single-cell RNA-sequencing, progenitor transfer and colony assays, we show that ThPOK controls monocyte-dendritic cell versus granulocyte lineage production during homeostatic differentiation, and serves as a brake for neutrophil maturation in granulocyte lineage-specified cells through transcriptional regulation of lineage-specific transcription factors and RNA via altered messenger RNA splicing to reprogram intron retention.</p>',
			'date' => '2023-08-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37474652',
			'doi' => '10.1038/s41590-023-01549-3',
			'modified' => '2023-08-01 13:37:22',
			'created' => '2023-08-01 15:59:38',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array()
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'ProductsApplication' => array(
		'id' => '2176',
		'product_id' => '2019',
		'application_id' => '19'
	)
)
$slugs = array(
	(int) 0 => 'western-blot-antibodies'
)
$applications = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'locale' => 'eng'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
	'id' => '568',
	'name' => 'Datasheet FLAG C15200209',
	'description' => 'Datasheet description',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/antibodies/Datasheet_FLAG_C15200209.pdf',
	'slug' => 'datasheet-flag-c15200209',
	'meta_keywords' => null,
	'meta_description' => null,
	'modified' => '2015-07-07 11:47:44',
	'created' => '2015-07-07 11:47:44',
	'ProductsDocument' => array(
		'id' => '294',
		'product_id' => '2019',
		'document_id' => '568'
	)
)
$publication = array(
	'id' => '4828',
	'name' => 'ThPOK is a critical multifaceted regulator of myeloid lineagedevelopment.',
	'authors' => 'Basu  J. et al.',
	'description' => '<p>The transcription factor ThPOK (encoded by Zbtb7b) is well known for its role as a master regulator of CD4 lineage commitment in the thymus. Here, we report an unexpected and critical role of ThPOK as a multifaceted regulator of myeloid lineage commitment, differentiation and maturation. Using reporter and knockout mouse models combined with single-cell RNA-sequencing, progenitor transfer and colony assays, we show that ThPOK controls monocyte-dendritic cell versus granulocyte lineage production during homeostatic differentiation, and serves as a brake for neutrophil maturation in granulocyte lineage-specified cells through transcriptional regulation of lineage-specific transcription factors and RNA via altered messenger RNA splicing to reprogram intron retention.</p>',
	'date' => '2023-08-01',
	'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37474652',
	'doi' => '10.1038/s41590-023-01549-3',
	'modified' => '2023-08-01 13:37:22',
	'created' => '2023-08-01 15:59:38',
	'ProductsPublication' => array(
		'id' => '7029',
		'product_id' => '2019',
		'publication_id' => '4828'
	)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/37474652" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => '',
	'meta_description' => 'FLAG Monoclonal Antibody  validated  in  WB and ELISA.  ',
	'meta_title' => 'FLAG Monoclonal Antibody | Diagenode',
	'product' => array(
		'Product' => array(
			'id' => '2019',
			'antibody_id' => '346',
			'name' => 'FLAG Antibody ',
			'description' => '<p>Monoclonal antibody raised in mouse against Flag&trade; (peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)), using a KLH-conjugated synthetic peptide.</p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200209-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="385" height="176" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 1. FLAG antibody western blot results </strong><br />Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 &mu;L. Samples were diluted 10-fold, transferred to nitrocellulose membrane and WB was performed with the FLAG monoclonal antibody.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200209-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="84" height="164" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 2. FLAG antibody western blot results </strong><br />Monoclonal Antibody to detect FLAGTM conjugated proteins detects both <g class="gr_ gr_14 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="14" data-gr-id="14">C terminal</g> linked and <g class="gr_ gr_15 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="15" data-gr-id="15">N terminal</g> linked FLAGTM tagged recombinant proteins by western blot.</small></p>
</div>
</div>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '100 µg',
			'catalog_number' => 'C15200209',
			'old_catalog_number' => '',
			'sf_code' => 'C15200209-D001-000526',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '435',
			'price_USD' => '440',
			'price_GBP' => '395',
			'price_JPY' => '68145',
			'price_CNY' => '',
			'price_AUD' => '1100',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'flag-monoclonal-antibody-classic-100-ug',
			'meta_title' => 'FLAG Monoclonal Antibody | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'FLAG Monoclonal Antibody  validated  in  WB and ELISA.  ',
			'modified' => '2022-01-05 15:36:13',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '346',
			'name' => 'FLAG monoclonal antibody - Classic',
			'description' => 'Antibody for the detection of FLAGTM recognizes FLAGTM and is optimally suited for monitoring the expression of FLAGTM tagged fusion proteins. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.',
			'clonality' => '',
			'isotype' => '',
			'lot' => '002',
			'concentration' => '1 μg/μl ',
			'reactivity' => 'Carboxy and amino terminal linked FLAG™ tagged recombinant proteins ',
			'type' => 'Monoclonal',
			'purity' => 'Protein A purified',
			'classification' => '',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:20,000 - 1:100,000</td>
<td></td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:2,000 - 1:20,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2023-05-04 10:48:01',
			'created' => '2015-07-16 16:57:09',
			'select_label' => '346 - FLAG monoclonal antibody - Classic (002 - 1 μg/μl  - Carboxy and amino terminal linked FLAG™ tagged recombinant proteins  - Protein A purified - Mouse)'
		),
		'Slave' => array(),
		'Group' => array(),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array()
	)
)
$language = 'en'
$meta_keywords = ''
$meta_description = 'FLAG Monoclonal Antibody  validated  in  WB and ELISA.  '
$meta_title = 'FLAG Monoclonal Antibody | Diagenode'
$product = array(
	'Product' => array(
		'id' => '2019',
		'antibody_id' => '346',
		'name' => 'FLAG Antibody ',
		'description' => '<p>Monoclonal antibody raised in mouse against Flag&trade; (peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)), using a KLH-conjugated synthetic peptide.</p>',
		'label1' => 'Validation Data',
		'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200209-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="385" height="176" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 1. FLAG antibody western blot results </strong><br />Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 &mu;L. Samples were diluted 10-fold, transferred to nitrocellulose membrane and WB was performed with the FLAG monoclonal antibody.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200209-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="84" height="164" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 2. FLAG antibody western blot results </strong><br />Monoclonal Antibody to detect FLAGTM conjugated proteins detects both <g class="gr_ gr_14 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="14" data-gr-id="14">C terminal</g> linked and <g class="gr_ gr_15 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="15" data-gr-id="15">N terminal</g> linked FLAGTM tagged recombinant proteins by western blot.</small></p>
</div>
</div>',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '100 µg',
		'catalog_number' => 'C15200209',
		'old_catalog_number' => '',
		'sf_code' => 'C15200209-D001-000526',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '435',
		'price_USD' => '440',
		'price_GBP' => '395',
		'price_JPY' => '68145',
		'price_CNY' => '',
		'price_AUD' => '1100',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'flag-monoclonal-antibody-classic-100-ug',
		'meta_title' => 'FLAG Monoclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'FLAG Monoclonal Antibody  validated  in  WB and ELISA.  ',
		'modified' => '2022-01-05 15:36:13',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'eng'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => '346',
		'name' => 'FLAG monoclonal antibody - Classic',
		'description' => 'Antibody for the detection of FLAGTM recognizes FLAGTM and is optimally suited for monitoring the expression of FLAGTM tagged fusion proteins. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.',
		'clonality' => '',
		'isotype' => '',
		'lot' => '002',
		'concentration' => '1 μg/μl ',
		'reactivity' => 'Carboxy and amino terminal linked FLAG™ tagged recombinant proteins ',
		'type' => 'Monoclonal',
		'purity' => 'Protein A purified',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:20,000 - 1:100,000</td>
<td></td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:2,000 - 1:20,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
		'storage_conditions' => '',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => '',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2023-05-04 10:48:01',
		'created' => '2015-07-16 16:57:09',
		'select_label' => '346 - FLAG monoclonal antibody - Classic (002 - 1 μg/μl  - Carboxy and amino terminal linked FLAG™ tagged recombinant proteins  - Protein A purified - Mouse)'
	),
	'Slave' => array(),
	'Group' => array(),
	'Related' => array(),
	'Application' => array(
		(int) 0 => array(
			'id' => '20',
			'position' => '10',
			'parent_id' => '40',
			'name' => 'ELISA',
			'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
</div>',
			'in_footer' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'slug' => 'elisa-antibodies',
			'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody',
			'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications',
			'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
			'modified' => '2016-01-13 12:21:41',
			'created' => '2014-07-08 08:13:28',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '19',
			'position' => '10',
			'parent_id' => '40',
			'name' => 'WB',
			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
			'in_footer' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'slug' => 'western-blot-antibodies',
			'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
			'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
			'modified' => '2016-04-26 12:44:51',
			'created' => '2015-01-07 09:20:00',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '32',
			'position' => '50',
			'parent_id' => '4',
			'name' => 'Epitope tags',
			'description' => '',
			'no_promo' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'protein-tag',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Protein tag,Polyclonal antibody,Monoclonal Protein tag antibodies',
			'meta_description' => 'Diagenode offers Polyclonal and Monoclonal Protein tag Antibodies',
			'meta_title' => 'Polyclonal and Monoclonal Protein tag Antibodies | Diagenode',
			'modified' => '2019-07-04 16:02:49',
			'created' => '2015-05-09 11:35:10',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		),
		(int) 1 => array(
			'id' => '103',
			'position' => '0',
			'parent_id' => '4',
			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'all-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
			'modified' => '2019-07-03 10:55:44',
			'created' => '2015-11-02 14:49:22',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '568',
			'name' => 'Datasheet FLAG C15200209',
			'description' => 'Datasheet description',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/antibodies/Datasheet_FLAG_C15200209.pdf',
			'slug' => 'datasheet-flag-c15200209',
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => '2015-07-07 11:47:44',
			'created' => '2015-07-07 11:47:44',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '250',
			'name' => 'product/antibodies/antibody.png',
			'alt' => 'Mouse IgG',
			'modified' => '2020-11-27 07:00:09',
			'created' => '2015-07-17 10:12:18',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '4828',
			'name' => 'ThPOK is a critical multifaceted regulator of myeloid lineagedevelopment.',
			'authors' => 'Basu  J. et al.',
			'description' => '<p>The transcription factor ThPOK (encoded by Zbtb7b) is well known for its role as a master regulator of CD4 lineage commitment in the thymus. Here, we report an unexpected and critical role of ThPOK as a multifaceted regulator of myeloid lineage commitment, differentiation and maturation. Using reporter and knockout mouse models combined with single-cell RNA-sequencing, progenitor transfer and colony assays, we show that ThPOK controls monocyte-dendritic cell versus granulocyte lineage production during homeostatic differentiation, and serves as a brake for neutrophil maturation in granulocyte lineage-specified cells through transcriptional regulation of lineage-specific transcription factors and RNA via altered messenger RNA splicing to reprogram intron retention.</p>',
			'date' => '2023-08-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37474652',
			'doi' => '10.1038/s41590-023-01549-3',
			'modified' => '2023-08-01 13:37:22',
			'created' => '2023-08-01 15:59:38',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array()
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'ProductsApplication' => array(
		'id' => '2176',
		'product_id' => '2019',
		'application_id' => '19'
	)
)
$slugs = array(
	(int) 0 => 'western-blot-antibodies'
)
$applications = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'locale' => 'eng'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
	'id' => '568',
	'name' => 'Datasheet FLAG C15200209',
	'description' => 'Datasheet description',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/antibodies/Datasheet_FLAG_C15200209.pdf',
	'slug' => 'datasheet-flag-c15200209',
	'meta_keywords' => null,
	'meta_description' => null,
	'modified' => '2015-07-07 11:47:44',
	'created' => '2015-07-07 11:47:44',
	'ProductsDocument' => array(
		'id' => '294',
		'product_id' => '2019',
		'document_id' => '568'
	)
)
$publication = array(
	'id' => '4828',
	'name' => 'ThPOK is a critical multifaceted regulator of myeloid lineagedevelopment.',
	'authors' => 'Basu  J. et al.',
	'description' => '<p>The transcription factor ThPOK (encoded by Zbtb7b) is well known for its role as a master regulator of CD4 lineage commitment in the thymus. Here, we report an unexpected and critical role of ThPOK as a multifaceted regulator of myeloid lineage commitment, differentiation and maturation. Using reporter and knockout mouse models combined with single-cell RNA-sequencing, progenitor transfer and colony assays, we show that ThPOK controls monocyte-dendritic cell versus granulocyte lineage production during homeostatic differentiation, and serves as a brake for neutrophil maturation in granulocyte lineage-specified cells through transcriptional regulation of lineage-specific transcription factors and RNA via altered messenger RNA splicing to reprogram intron retention.</p>',
	'date' => '2023-08-01',
	'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37474652',
	'doi' => '10.1038/s41590-023-01549-3',
	'modified' => '2023-08-01 13:37:22',
	'created' => '2023-08-01 15:59:38',
	'ProductsPublication' => array(
		'id' => '7029',
		'product_id' => '2019',
		'publication_id' => '4828'
	)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/37474652" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => '',
	'meta_description' => 'FLAG Monoclonal Antibody  validated  in  WB and ELISA.  ',
	'meta_title' => 'FLAG Monoclonal Antibody | Diagenode',
	'product' => array(
		'Product' => array(
			'id' => '2019',
			'antibody_id' => '346',
			'name' => 'FLAG Antibody ',
			'description' => '<p>Monoclonal antibody raised in mouse against Flag&trade; (peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)), using a KLH-conjugated synthetic peptide.</p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200209-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="385" height="176" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 1. FLAG antibody western blot results </strong><br />Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 &mu;L. Samples were diluted 10-fold, transferred to nitrocellulose membrane and WB was performed with the FLAG monoclonal antibody.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200209-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="84" height="164" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 2. FLAG antibody western blot results </strong><br />Monoclonal Antibody to detect FLAGTM conjugated proteins detects both <g class="gr_ gr_14 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="14" data-gr-id="14">C terminal</g> linked and <g class="gr_ gr_15 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="15" data-gr-id="15">N terminal</g> linked FLAGTM tagged recombinant proteins by western blot.</small></p>
</div>
</div>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '100 µg',
			'catalog_number' => 'C15200209',
			'old_catalog_number' => '',
			'sf_code' => 'C15200209-D001-000526',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '435',
			'price_USD' => '440',
			'price_GBP' => '395',
			'price_JPY' => '68145',
			'price_CNY' => '',
			'price_AUD' => '1100',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'flag-monoclonal-antibody-classic-100-ug',
			'meta_title' => 'FLAG Monoclonal Antibody | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'FLAG Monoclonal Antibody  validated  in  WB and ELISA.  ',
			'modified' => '2022-01-05 15:36:13',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '346',
			'name' => 'FLAG monoclonal antibody - Classic',
			'description' => 'Antibody for the detection of FLAGTM recognizes FLAGTM and is optimally suited for monitoring the expression of FLAGTM tagged fusion proteins. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.',
			'clonality' => '',
			'isotype' => '',
			'lot' => '002',
			'concentration' => '1 μg/μl ',
			'reactivity' => 'Carboxy and amino terminal linked FLAG™ tagged recombinant proteins ',
			'type' => 'Monoclonal',
			'purity' => 'Protein A purified',
			'classification' => '',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:20,000 - 1:100,000</td>
<td></td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:2,000 - 1:20,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2023-05-04 10:48:01',
			'created' => '2015-07-16 16:57:09',
			'select_label' => '346 - FLAG monoclonal antibody - Classic (002 - 1 μg/μl  - Carboxy and amino terminal linked FLAG™ tagged recombinant proteins  - Protein A purified - Mouse)'
		),
		'Slave' => array(),
		'Group' => array(),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array()
	)
)
$language = 'en'
$meta_keywords = ''
$meta_description = 'FLAG Monoclonal Antibody  validated  in  WB and ELISA.  '
$meta_title = 'FLAG Monoclonal Antibody | Diagenode'
$product = array(
	'Product' => array(
		'id' => '2019',
		'antibody_id' => '346',
		'name' => 'FLAG Antibody ',
		'description' => '<p>Monoclonal antibody raised in mouse against Flag&trade; (peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)), using a KLH-conjugated synthetic peptide.</p>',
		'label1' => 'Validation Data',
		'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200209-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="385" height="176" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 1. FLAG antibody western blot results </strong><br />Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 &mu;L. Samples were diluted 10-fold, transferred to nitrocellulose membrane and WB was performed with the FLAG monoclonal antibody.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200209-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="84" height="164" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 2. FLAG antibody western blot results </strong><br />Monoclonal Antibody to detect FLAGTM conjugated proteins detects both <g class="gr_ gr_14 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="14" data-gr-id="14">C terminal</g> linked and <g class="gr_ gr_15 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="15" data-gr-id="15">N terminal</g> linked FLAGTM tagged recombinant proteins by western blot.</small></p>
</div>
</div>',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '100 µg',
		'catalog_number' => 'C15200209',
		'old_catalog_number' => '',
		'sf_code' => 'C15200209-D001-000526',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '435',
		'price_USD' => '440',
		'price_GBP' => '395',
		'price_JPY' => '68145',
		'price_CNY' => '',
		'price_AUD' => '1100',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'flag-monoclonal-antibody-classic-100-ug',
		'meta_title' => 'FLAG Monoclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'FLAG Monoclonal Antibody  validated  in  WB and ELISA.  ',
		'modified' => '2022-01-05 15:36:13',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'eng'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => '346',
		'name' => 'FLAG monoclonal antibody - Classic',
		'description' => 'Antibody for the detection of FLAGTM recognizes FLAGTM and is optimally suited for monitoring the expression of FLAGTM tagged fusion proteins. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.',
		'clonality' => '',
		'isotype' => '',
		'lot' => '002',
		'concentration' => '1 μg/μl ',
		'reactivity' => 'Carboxy and amino terminal linked FLAG™ tagged recombinant proteins ',
		'type' => 'Monoclonal',
		'purity' => 'Protein A purified',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:20,000 - 1:100,000</td>
<td></td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:2,000 - 1:20,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
		'storage_conditions' => '',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => '',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2023-05-04 10:48:01',
		'created' => '2015-07-16 16:57:09',
		'select_label' => '346 - FLAG monoclonal antibody - Classic (002 - 1 μg/μl  - Carboxy and amino terminal linked FLAG™ tagged recombinant proteins  - Protein A purified - Mouse)'
	),
	'Slave' => array(),
	'Group' => array(),
	'Related' => array(),
	'Application' => array(
		(int) 0 => array(
			'id' => '20',
			'position' => '10',
			'parent_id' => '40',
			'name' => 'ELISA',
			'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
</div>',
			'in_footer' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'slug' => 'elisa-antibodies',
			'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody',
			'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications',
			'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
			'modified' => '2016-01-13 12:21:41',
			'created' => '2014-07-08 08:13:28',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '19',
			'position' => '10',
			'parent_id' => '40',
			'name' => 'WB',
			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
			'in_footer' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'slug' => 'western-blot-antibodies',
			'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
			'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
			'modified' => '2016-04-26 12:44:51',
			'created' => '2015-01-07 09:20:00',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '32',
			'position' => '50',
			'parent_id' => '4',
			'name' => 'Epitope tags',
			'description' => '',
			'no_promo' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'protein-tag',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Protein tag,Polyclonal antibody,Monoclonal Protein tag antibodies',
			'meta_description' => 'Diagenode offers Polyclonal and Monoclonal Protein tag Antibodies',
			'meta_title' => 'Polyclonal and Monoclonal Protein tag Antibodies | Diagenode',
			'modified' => '2019-07-04 16:02:49',
			'created' => '2015-05-09 11:35:10',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		),
		(int) 1 => array(
			'id' => '103',
			'position' => '0',
			'parent_id' => '4',
			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'all-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
			'modified' => '2019-07-03 10:55:44',
			'created' => '2015-11-02 14:49:22',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '568',
			'name' => 'Datasheet FLAG C15200209',
			'description' => 'Datasheet description',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/antibodies/Datasheet_FLAG_C15200209.pdf',
			'slug' => 'datasheet-flag-c15200209',
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => '2015-07-07 11:47:44',
			'created' => '2015-07-07 11:47:44',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '250',
			'name' => 'product/antibodies/antibody.png',
			'alt' => 'Mouse IgG',
			'modified' => '2020-11-27 07:00:09',
			'created' => '2015-07-17 10:12:18',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '4828',
			'name' => 'ThPOK is a critical multifaceted regulator of myeloid lineagedevelopment.',
			'authors' => 'Basu  J. et al.',
			'description' => '<p>The transcription factor ThPOK (encoded by Zbtb7b) is well known for its role as a master regulator of CD4 lineage commitment in the thymus. Here, we report an unexpected and critical role of ThPOK as a multifaceted regulator of myeloid lineage commitment, differentiation and maturation. Using reporter and knockout mouse models combined with single-cell RNA-sequencing, progenitor transfer and colony assays, we show that ThPOK controls monocyte-dendritic cell versus granulocyte lineage production during homeostatic differentiation, and serves as a brake for neutrophil maturation in granulocyte lineage-specified cells through transcriptional regulation of lineage-specific transcription factors and RNA via altered messenger RNA splicing to reprogram intron retention.</p>',
			'date' => '2023-08-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37474652',
			'doi' => '10.1038/s41590-023-01549-3',
			'modified' => '2023-08-01 13:37:22',
			'created' => '2023-08-01 15:59:38',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array()
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'ProductsApplication' => array(
		'id' => '2176',
		'product_id' => '2019',
		'application_id' => '19'
	)
)
$slugs = array(
	(int) 0 => 'western-blot-antibodies'
)
$applications = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'locale' => 'eng'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
	'id' => '568',
	'name' => 'Datasheet FLAG C15200209',
	'description' => 'Datasheet description',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/antibodies/Datasheet_FLAG_C15200209.pdf',
	'slug' => 'datasheet-flag-c15200209',
	'meta_keywords' => null,
	'meta_description' => null,
	'modified' => '2015-07-07 11:47:44',
	'created' => '2015-07-07 11:47:44',
	'ProductsDocument' => array(
		'id' => '294',
		'product_id' => '2019',
		'document_id' => '568'
	)
)
$publication = array(
	'id' => '4828',
	'name' => 'ThPOK is a critical multifaceted regulator of myeloid lineagedevelopment.',
	'authors' => 'Basu  J. et al.',
	'description' => '<p>The transcription factor ThPOK (encoded by Zbtb7b) is well known for its role as a master regulator of CD4 lineage commitment in the thymus. Here, we report an unexpected and critical role of ThPOK as a multifaceted regulator of myeloid lineage commitment, differentiation and maturation. Using reporter and knockout mouse models combined with single-cell RNA-sequencing, progenitor transfer and colony assays, we show that ThPOK controls monocyte-dendritic cell versus granulocyte lineage production during homeostatic differentiation, and serves as a brake for neutrophil maturation in granulocyte lineage-specified cells through transcriptional regulation of lineage-specific transcription factors and RNA via altered messenger RNA splicing to reprogram intron retention.</p>',
	'date' => '2023-08-01',
	'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37474652',
	'doi' => '10.1038/s41590-023-01549-3',
	'modified' => '2023-08-01 13:37:22',
	'created' => '2023-08-01 15:59:38',
	'ProductsPublication' => array(
		'id' => '7029',
		'product_id' => '2019',
		'publication_id' => '4828'
	)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/37474652" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Contact Information

First name

Last name

Company

Phone number

City

Country

State

Email verification^*

Comment

Events

See all events

Site map | Contact us | Conditions of sales | Conditions of purchase | Privacy policy